• secretion
  • Because of its highly nonpolar character and propensity to self-aggregate, it has been suggested that mechanisms limiting self-aggregation must also be present during the transit of tropoelastin through the cell prior to secretion. (sigmaaldrich.com)
  • The resulting fibroblast secretion of this condition causes accumulation of abnormal tropoelastin clumps rather than orderly arrays, in the dermis. (news-medical.net)
  • Although HS chains are not required for correct folding and secretion of the protein, lack of HS or decreased sulfation can decrease perlecan's ability to interact with matrix proteins. (wikipedia.org)
  • proteoglycans
  • E xtracellular matrix of animal tissues is a molecular network mainly composed of proteins, proteoglycans, glycosaminoglycans, and glycoproteins, that comine together to form a diverse set of extracellular matrices. (uvigo.es)
  • The association with these matrix structures is mediated by its ability to interact with numerous extracellular matrix constituents including fibronectin, proteoglycans, laminins and tropoelastin. (genenames.org)
  • The core protein mRNA levels of proteoglycans were not affected, thus the decrease in GAG chains was as a result of some other factor, which in this case turned out to be a decrease in expression of sulfate transferase enzymes, which play a key role in GAG biosynthesis. (wikipedia.org)
  • exon
  • The protein consists of alternating hydrophobic and hydrophilic domains, which are encoded by separate exons, so that the domain structure of tropoelastin reflects the exon organization of the gene. (wikipedia.org)
  • These transcripts vary only in the first exon and code for the same protein which is initiated in the third exon The seventh intron of the egfl7 gene contains a miRNA site for miR-126 and miR-126. (wikipedia.org)
  • mRNA
  • The expression of tropoelastin mRNA is highly regulated under at least eight different transcription start sites. (wikipedia.org)
  • The alternative 2.0-kb mRNA encodes a beta-galactosidase-related protein (S-Gal) that is only 546 amino acids long and that has no enzymatic activity. (wikipedia.org)
  • recombinant
  • Elastagen Pty Limited , a clinical stage company developing medical device products based on recombinant tropoelastin, today announced that it has entered into a definitive agreement under which Allergan , a leading global biopharmaceutical company, has agreed to acquire Elastagen for an upfront payment of US$95M plus contingent, commercial payments. (biospace.com)
  • Systems for the recombinant production of spider silk proteins in E . coli have been developed earlier. (patent-de.com)
  • By expressing a recombinant form of the N-terminal domain I of the protein and demonstrating that digestion of the peptide with either heparanase or chondroitinase did not lead to complete loss of the peptide's activity, it was shown that chondroitin sulfate chains can be added to human perlecan. (wikipedia.org)
  • This was in agreement with previous data showing chondroitin sulfate GAG chains attached to bovine perlecan produced by chondrocytes and that recombinant human domain I protein was glycosylated with both heparan and chondroitin sulfate chains when expressed in Chinese Hamster Ovary cells. (wikipedia.org)
  • polymers
  • Spider silks are protein polymers that display extraordinary physical properties. (patent-de.com)
  • The protein purification aspect of ELPs is supported by the ability of these polymers to undergo morphological changes at certain temperatures, enabling the ELPs bound to specific proteins to be separated out from the rest of the solution via experimental techniques such as centrifugation. (wikipedia.org)
  • aggregates
  • Both proteins also modify the kinetics of self-assembly of tropoelastin in an in vitro system, consistent with a role in attenuating the premature intracellular self-aggregation of tropoelastin through a mechanism that limits the growth and maturation of aggregates. (sigmaaldrich.com)
  • Play media Although ELPs generally form reversible spherical aggregates due to their proline and glycine content, there is a possibility that, under certain conditions such as exceedingly high temperatures, ELPs will form amyloids, or irreversible aggregates of insoluble protein. (wikipedia.org)
  • modulate
  • Thus, perlecan core protein and HS chains could modulate matrix assembly, cell proliferation, lipoprotein binding and cell adhesion. (wikipedia.org)
  • neuraminidase
  • Plasma levels of neuraminidase and the amount of TGF-β in mesenteric arteries were elevated in mice fed a WD, while ex vivo , cultured vascular smooth muscle cells exposed to neuraminidase secreted greater amounts of tropoelastin and TGF-β than those exposed to vehicle. (frontiersin.org)
  • The elastin receptor complex includes S-Gal, neuraminidase and Cathepsin A. When elastin-derived peptides bind to the S-Gal protein then the associated neuraminidase enzyme activity is activated and responding cells can have altered signal transduction involving extracellular signal-regulated kinases and regulated matrix metallopeptidase production. (wikipedia.org)
  • found
  • The presence of lipids in the residue was found to be a consequence of damage to the plasma membrane during the rehydration process and the increased cell stress was also apparent by the amount of Hsp12 in the protein residue. (royalsocietypublishing.org)
  • C ollagens are a family of proteins found in animals, so abundant that they can be up to 25 to 35 % of the total protein content of the body. (uvigo.es)
  • Although engineered and modified in a laboratory setting, ELPs share structural characteristics with intrinsically disordered proteins (IDPs) naturally found in the body, such as tropoelastin, from which ELPs were given their name. (wikipedia.org)
  • Elastin is initially present as newly synthesized tropoelastin which can be found in association with S-Gal. (wikipedia.org)
  • The Egfl7 protein (29 kDa) is composed of several putative domains: a putative cleavable signal peptide at the N-terminal end, an EMI domain, found on extracellular matrix proteins, two EGF-like domains and a leucine and valine rich C-terminal region. (wikipedia.org)
  • A large number of extracellular matrix proteins have been found to contain variations of the epidermal growth factor (EGF) domain and have been implicated in functions as diverse as blood coagulation, activation of complement and determination of cell fate during development. (wikipedia.org)
  • conformation
  • Effect of low molecular weight additives on immobilization strength, activity, and conformation of protein immobilized on PVC and UHMWPE. (jove.com)
  • Each tropoelastin consists of a string of 36 small domains, each weighing about 2 kDa in a random coil conformation. (wikipedia.org)
  • mechanism
  • However, details about the nature of the interactions between these proteins as well as about the mechanism by which they may act to limit self-aggregation are lacking. (sigmaaldrich.com)
  • highly
  • One of the major reasons that elastin can withstand high levels of stress in the body without experiencing any physical deformation is that the underlying tropoelastin contains domains that are highly hydrophobic. (wikipedia.org)
  • molecular
  • Perlecan consists of a core protein of molecular weight 470 kDa to which three long chains (each approximately 70-100 kDa) of glycosaminoglycans (often heparan sulfate, HS but can be chondroitin sulfate, CS) are attached. (wikipedia.org)