Proteins protein 2 caco 2 cells. Medical search. Frequent questions

We conclude that LKB1 can induce complete polarity in intestinal epithelial cells. (nih.gov)
Adenine has been suggested to induce cell cycle arrest in cancer cells, leading to cell death [ 9 , 10 ]. (hindawi.com)
The two strains mediate adhesion by different mechanisms and, when combined, synergically induce the expression of Caco-2 tight junction proteins. (frontiersin.org)
Mice lacking Ern2 had a dysbiotic microbial community that failed to induce goblet cell development and increased susceptibility to colitis when transferred into germ-free wild type mice. (jci.org)
The inflammatory and immune response severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection can induce can prove deadly, and researchers from Harvard Medical School have been investigating how much of this response is specific to SARS-CoV-2. (news-medical.net)

The data showed that adenine activated AMP-activated protein kinase (AMPK) signaling contributing to autophagic cell death through mTOR in both colon cancer cell lines. (hindawi.com)
Involvement of AMP-activated protein kinase (AMPK) in anticancer property of adenine was also investigated. (hindawi.com)
RNA-Seq analysis and the data from experimental models revealed that neutrophilic NCF1-dependent ROS promoted alcoholic hepatitis (AH) by inhibiting AMP-activated protein kinase (a key regulator of lipid metabolism) and microRNA-223 (a key antiinflammatory and antifibrotic microRNA). (jci.org)
Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. (thermofisher.com)

ERN2 acted by splicing Xbp1 mRNA to expand ER function and prevent ER stress in goblet cells. (jci.org)
CXCR1 is expressed in the human IEC line Caco-2 BBE [ 3 ], which is in agreement with our own microarray data using Caco-2 BBE cell mRNA (see below). (biomedcentral.com)

The intestinal barrier is made up of a layer of epithelial cells, preventing harmful pathogens and toxins from entering the system (i.e., leaky gut), and is critical for fluid and electrolyte secretion. (kemin.com)
Clearance of small intestinal crypts involves goblet cell mucus secretion by intracellular granule rupture and enterocyte ion transport. (gu.se)
In the Caco-2 BBE model of polarized villus-like epithelium, apical stimulation with TLR2 and TLR5 ligands resulted in the apical secretion of IL-8. (biomedcentral.com)
Intestinal epithelial cells (IECs) play a key role in the inflammatory response to colonizing or invading microorganisms via the secretion of interleukin-8 (IL-8). (biomedcentral.com)
However, it is not known if CXCR1 is expressed on the apical or basolateral poles in polarized Caco-2 BBE cells or human intestinal tissue or whether the direction of TLR signalling influences the direction of IL-8 secretion. (biomedcentral.com)
Here we investigated IL-8 secretion in response to TLR signalling using intestinal Caco-2 BBE cells grown in the Transwell system. (biomedcentral.com)
Stimulation of polarized Caco-2 BBE cells with Pam2CSK4 and Pam3CSK4, agonists for TLR2/6 and TLR2/1 from the apical or basolateral sides induced secretion of IL-8 predominantly into the apical compartment regardless of the location of the stimulus, although basolateral stimulation induced smaller amounts of IL-8 compared to apical stimulation. (biomedcentral.com)
Peptide hydrolysate significantly decreased secretion of pro-inflammatory cytokine IL-8 in lipopolysaccharide (LPS)-stimulated human colon epithelial HT-29 cells. (calbinotox.fr)

Caprin-1 is a protein that in humans is encoded by the CAPRIN1 gene. (wikipedia.org)
New research shows that humans may have difficulty in taking up certain proteins from plant-based products. (cosmosmagazine.com)
While humans lack the enzyme to digest cellulose from plants, how good are we at taking up proteins from plant-based meat analogues versus animal-derived meat? (cosmosmagazine.com)
Glycosphingolipids (GSLs) are a subclass of glycolipids that are found in cell membranes of various organisms ranging from bacteria to humans. (springer.com)
Only two of these genotypes, A and B (Nash Groups 1/2 and 3 ( 150 , 151 ) are found in humans. (antimicrobe.org)

It is known that GSLs interact with both intracellular as well as exogenous proteins and are critical for membrane organisation, signalling, and recognition events. (springer.com)
It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. (thermofisher.com)

In the present work, the effects on cell inflammation of proteins and their peptides obtained by simulated gastro-intestinal digestion have been studied using cultivated intestinal Caco-2 cells, whose immune response was triggered by IL-1. (cancer-ecosystem.com)

Disruption of plant plasma membrane by Nep1-like proteins in pathogen/plant interactions. (ki.si)
It is a region where the plasma membrane of adjacent cells forms a series of contacts that appears to completely occlude the extracellular space as observed by transmission EM. (rupress.org)

intestinal epithelial cells (IECs) present in the metabolic state of low oxygen tension is called "physiological hypoxia. (microarraystation.com)

We have now constructed intestinal epithelial cell lines in which inducible STRAD activates LKB1. (nih.gov)
To study the role of occludin in the formation of the tight junction seal, synthetic peptides (OCC1 and OCC2) corresponding to the two putative extracellular domains of occludin were assayed for their ability to alter tight junctions in Xenopus kidney epithelial cell line A6. (rupress.org)
Despite the increased paracellular permeability, there were no changes in gross epithelial cell morphology as determined by scanning EM. (rupress.org)

The anti-inflammatory activity of peptide hydrolysate was investigated under inflammatory conditions in two cell models. (calbinotox.fr)

Researchers decided to measure the levels of peptides (protein chains) before and after digestion and absorption of meat analogues (MA) and chicken breast (CB). (cosmosmagazine.com)
Upon further digestion, the CB had a higher degree of hydrolysis compared to the MA - 210mg/g of water-soluble proteins were broken down from the CB, compared to 180mg/g from the MA. (cosmosmagazine.com)
This study aimed to characterize bio-compounds from peanut skin (Arachis hypogaea) and their bioactivity (antioxidant activity, inhibition of lipase, and carbohydrase enzymes) and to evaluate their anti-proliferative properties in colorectal cancer cells (HCT116) upon in vitro digestion. (bvsalud.org)
Purification of Pseudocereal Protein Fractions and Their In Vitro Digestion The isolation procedure we adopted allowed us, as a first step, to obtain a water-soluble fraction, namely albumin, and a salt-soluble fraction, corresponding to globulins. (cancer-ecosystem.com)
We hypothesized that peptides released from the surface proteins of this bacterium during digestion could be implied in this activity. (calbinotox.fr)

Therefore, the main objective of this study was to elucidate how iron-depleted, iron-saturated, and manganese-saturated forms of lactoferrin regulate intestinal barrier function via interactions with epithelial cells and macrophages. (springer.com)
The researchers established a coculture model using ex vivo blood immunocytes that were in direct contact with virus infected epithelial cells in order to assess immunocyte interactions with SARS-CoV-2 infected cells. (news-medical.net)
Being typically a minor component of the cell membrane, they are of utmost importance for biological functions that rely on lipid-lipid and lipid-protein interactions (Schnaar and Kinoshita 2015 ). (springer.com)
Oligo-pyrrolinone-pyrrolidines (generic structure 1) have the potential to interfere with protein-protein interactions (PPIs), but to reduce this to practice it is necessary to be able to synthesize these structures with a variety of different side chains corresponding to genetically encoded proteins. (tamu.edu)

Immunohistochemistry analyses of explanted livers identified two SAH phenotypes despite a similar clinical presentation, one with high intrahepatic neutrophils (Neuhi), but low levels of CD8+ T cells, and vice versa. (jci.org)
We developed a simple aqueous extraction method for ground seeds and analysed their composition by mineral, protein and monosaccharide analyses. (peerj.com)
Compositional analyses revealed the presence of polysaccharides, proteins and essential minerals in the aqueous extracts and in vitro assays showed sunflower had the highest antioxidant activity. (peerj.com)
Transcriptome analyses revealed that Caco-2 BBE cells respond to stimulation with IL-8 supporting the hypothesis that IL-8 induces G protein-coupled receptor signalling. (biomedcentral.com)

It is of interest to explore the inhibitory effect of adenine on the growth of colorectal cancer cells and to determine the underlying mechanism. (hindawi.com)
We explored the possible mechanisms underlying the anticancer activity of adenine in colorectal cancer cells. (hindawi.com)

In the present study, we investigated the anticancer effects of adenine and the underlying mechanism in colon cancer cells. (hindawi.com)
Adenine significantly inhibited the viability of colon cancer cells, HT29 and Caco-2 cells, in a dose-dependent manner. (hindawi.com)
Our findings suggest that adenine inhibits the growth of colon cancer cells. (hindawi.com)
Anticancer activity of adenine in colon cancer cells is attributable to the activation of apoptotic signaling and in turn the AMPK/mTOR pathway. (hindawi.com)
In this study, we investigated the effects of adenine against the proliferation of colon cancer cells. (hindawi.com)
Using conventionally raised and germ-free Ern2-/- mice, we found that ERN2 was required for microbiota-induced goblet cell maturation and mucus barrier assembly in the colon. (jci.org)
These digests were then put through an absorption process that simulates what happens in our intestines, using a permeation layer of Caco-2 cells that are found in the colon of the large intestine. (cosmosmagazine.com)
The CXCR1 receptor for IL-8 was expressed only on the apical membrane of Caco-2 BBE cells and differentiated epithelial cells in the human small intestine and colon. (biomedcentral.com)
Interestingly, IL-8 has been measured in the colon milieu of healthy volunteers using a dialysis bag inserted in the rectum [ 9 ] suggesting that it might be apically secreted by colonic epithelial cells. (biomedcentral.com)

In vitro characterization revealed that the strains tolerate gastric and bile challenges and display a great adhesion capacity to human intestinal cells. (frontiersin.org)
Human colorectal adenocarcinoma cell line Caco-2, known to be susceptible to SARS-CoV-2 infection, was used in place of primary lung epithelial cells, as these are known to be difficult to expand and manipulate. (news-medical.net)
This study from the Ohio State University (US), published in the Journal of Agricultural and Food Chemistry , shows how proteins from plant-based meat substitutes may not be as accessible to human cells as proteins from animal meat. (cosmosmagazine.com)
Human are recommended to consume between 0.8 grams of protein per kilogram of body weight to be able to meet basic nutritional requirements. (cosmosmagazine.com)
Lactobacillus rhamnosus is an anaerobic facultative heterofermentative rod-shaped bacterium that can live in different parts of the human body, including the gastrointestinal tract (GIT) 2 . (nature.com)
Cumulative clinical evidence shows alterations in the ubiquitylation of cell-cycle regulators in the aetiology of many human malignancies. (scienceopen.com)
These results show that IL-8 induces autocrine signalling via an apical CXCR1 in Caco-2 BBE intestinal epithelial cells and that this receptor is also expressed on the apical surface of differentiated human intestinal epithelial cells in vivo , suggesting an autocrine function for IL-8 secreted in the lumen. (biomedcentral.com)
Additionally, we determined the localization of the CXCR1 receptor in Caco-2 BBE cells and human intestinal tissue samples and investigated the role of IL-8 autocrine signalling using transcriptomics. (biomedcentral.com)

The protein encoded by this gene is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. (thermofisher.com)

None of the tested forms induced a pro-inflammatory response in Caco-2 cells or in macrophages either. (springer.com)
The peptide hydrolysates of both strains exhibited anti-inflammatory action through the modulation of pro-inflammatory mediators in LPS-stimulated THP-1 macrophages (COX-2, Pro-IL-1β, IL-1β, and IL-8) and LPS-stimulated HT-29 cells (IL-8). (calbinotox.fr)
and COX-2 in LPS-stimulated THP-1 macrophages. (calbinotox.fr)

Extraction procedures are optimized to precipitate and remove proteins and nucleic acids while maximizing solubilization of GSLs along with other lipids. (springer.com)
Plasma biomarkers - lipids, glucometabolic markers, high-sensitivity C-reactive protein (hs-CRP), interleukin-6 (IL-6), interleukin-10 (IL-10), monocyte chemoattractant protein-1 (MCP-1), and tumor necrosis factor-α (TNF-α) - and RBC palmitic and stearic fatty acids were analyzed. (bvsalud.org)
When free radicals are overproduced or the cellular defenses are impaired, biomolecules such as lipids, proteins, and DNA may be damaged by oxidative stress [20], ultimately leading to pathological conditions. (cancer-ecosystem.com)

Here we investigated whether polarized monolayers of intestinal epithelial cells might regulate inflammatory responses by secreting IL-8 in a vectorial fashion (i.e. apical versus basolateral) depending on the location of the TLR stimulus. (biomedcentral.com)

Overall, seed proteins can express these bioactivities mainly after hydrolysis. (cancer-ecosystem.com)
Our previous study displayed that this activity could be partially due to peptide(s) generated by trypsin hydrolysis of the surface proteins of S. thermophilus LMD-9. (calbinotox.fr)
Consequently, we prepared a peptide hydrolysate by shaving and hydrolysis of surface proteins using trypsin, and the origin of peptides was checked by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. (calbinotox.fr)

IL-8 can signal through two receptors, CXCR1 and CXCR2 both of which are members of the G-protein-coupled receptor (GPCR) family [ 2 ]. (biomedcentral.com)

Modelling the role of membrane mechanics in cell adhesion on titanium oxide nanotubes. (ki.si)
Occludin, the putative tight junction integral membrane protein, is an attractive candidate for a protein that forms the actual sealing element of the tight junction. (rupress.org)
Whereas the trisaccharidic head group of Gb 3 defines the specificity of Shiga toxin binding, the lipophilic part composed of sphingosine and different fatty acids is suggested to determine its localization within membranes impacting membrane organisation and protein binding eventually leading to protein internalisation. (springer.com)
Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). (thermofisher.com)

By supporting tight junctions, or multi-protein cells that tightly bind the epithelial cells within the stomach lining, the immunity response of animals can be improved. (kemin.com)

Following this the researchers compared monocyte responses to epithelial cells infected with SARS-CoV-2 compared to other viruses, including the respiratory pathogen IAV and EBOV, a hemorrhagic disease associated with strong inflammation. (news-medical.net)

Cell viability was measured using the MTT assay. (hindawi.com)
Inhibition of cell viability was assessed with focus on apoptosis transcriptionally and translationally. (hindawi.com)
Cell viability assays were performed on Caco-2 and IEC-6 intestinal epithelial cells using increasing doses of the prepared extracts. (peerj.com)
However, differences in extract composition and antioxidant properties could not be directly related to the observed increase in cell viability suggesting that other components in the extracts may be responsible. (peerj.com)
For cell viability in HCT116 cells, lower concentrations of P1 were found for IC50 compared to P2. (bvsalud.org)

OBJECTIVE: To evaluate the association of red blood cell (RBC) SFA (palmitic and stearic acids) with circulating inflammatory and endothelial dysfunction biomarkers. (bvsalud.org)
Aberrant functioning of serine proteases may contribute to the development of disorders Ang derived from inflammatory cell activation that lead to immunological problems and excessive activation of inflammation [34]. (cancer-ecosystem.com)

In Caco-2 cell experiments, the compound displayed modest permeability suggesting that it may show limited bioavailability via oral routes. (scienceopen.com)

A driving force of the cell cycle is the activation of cyclin-dependent kinases (CDKs), the activities of which are controlled by the ubiquitin-mediated proteolysis of key regulators such as cyclins and CDK inhibitors. (scienceopen.com)

Adenine forms several biological compounds involved in a variety of cellular physiological processes such as adenosine triphosphate (ATP) in cellular respiration and deoxyribonucleic acid (DNA) in protein synthesis. (hindawi.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. (thermofisher.com)
In neutrophils, IL-8 binding to CXCR1 triggers G protein-coupled signalling and formation of second messengers that mediate cellular migration, exocytosis of effectors and a respiratory burst to facilitate oxygen-dependent killing of phagocytosed microorganisms [ 2 ]. (biomedcentral.com)

Levels of phosphorylation and protein expression were determined using western blotting. (hindawi.com)
By conducting an in vitro CRC cell line study through the knockdown of EGFR expression, we analyzed cell proliferation, colony formation and migration. (oncotarget.com)
Here, we aim to identify the PP1, PP8, and pp22 and protein expression patterns placenta and trophoblast them to reveal the involvement of the potential complications of pregnancy. (microarraystation.com)
The encoded nuclear protein is involved in the early development of the pancreas and plays a major role in glucose-dependent regulation of insulin gene expression. (thermofisher.com)
and the protein expression levels of Pro-IL-1? (calbinotox.fr)

These have an intricated and complex set of mechanics related to immune response, cell mobility and signaling pathways, and the scientists suggest that the changes cells undergo when exposed to infected cells are profound and far-reaching. (news-medical.net)

Finally, an exploratory, observational, pilot study involving 36 infants diagnosed with at least one FGID (infant colic and/or functional constipation) showed the probiotic formula was well tolerated and FGID severity was significantly reduced after 14 days of treatment with the 2 strains. (frontiersin.org)

Upon LKB1 activation, single cells rapidly remodel their actin cytoskeleton to form an apical brush border. (nih.gov)
The tight junction, also known as zonula occludens , is the apical-most component of the junctional complex of epithelial and endothelial cells. (rupress.org)

The results of recently conducted clinical trials suggest that lactoferrin, an iron-binding protein that is abundant in mammalian colostrum and milk, may be an effective agent in preventing sepsis in newborns. (springer.com)
Albumins and globulins are the most abundant seed proteins of pseudocereals. (cancer-ecosystem.com)

A final amino acid concentration was measured, showing ~300 μmol/mL in the MA versus ∼430 μmol/mL for the CB, indicating a great CB peptide uptake from the intestinal cells into the bloodstream. (cosmosmagazine.com)

However, the mechanism by which adenine inhibits the proliferation of cancer cells is sketchy. (hindawi.com)

We have previously reported the identification and characterization of an LKB1-specific adaptor protein, STRAD, which activates LKB1 and translocates it from nucleus to cytoplasm. (nih.gov)

Whole cell extracts (30 µg lysate) of HeLa (Lane 1), SH-SY5Y (Lane 2), A549 (Lane 3), A-431 (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). (thermofisher.com)
Peanut skin was digested in two sequential phases, and the final content, named phase-1 (P1) and phase-2 (P2) extracts, was evaluated. (bvsalud.org)

Towards in silico CLIP-seq : predicting protein-RNA interaction via sequence-to-signal learning. (ki.si)

Using flow cytometry and immunofluorescence they found that nucleocapsid protein from the virus was expressed in approximately 50% of Caco2 cells. (news-medical.net)

The scope of the special issue is to summarize and enlarge the knowledge in signalling processes and networks in diverse cells and tissues. (mdpi.com)
For the analysis of GSLs, they are extracted from tissues and cells using organic solvents. (springer.com)

J Cell Biol (1997) 136 (2): 399-409. (rupress.org)

Our results show that CNCM I-3690 can (i) physically maintain modulated goblet cells and the mucus layer and (ii) counteract changes in local and systemic lymphocytes. (nature.com)
It presents a functional unit of a physical barrier consisting of a mucus layer and a monolayer of epithelial cells and of a mucosal lymphoid system that together efficiently discriminate between pathogenic and commensal microorganisms 16 . (nature.com)

In this study, we investigated the metabolic stability, metabolite production, metabolic pathways and plasma protein binding (PPB) of FHND6081 along with its absorption, tissue distribution, excretion (ADME) and pharmacokinetics (PK) in animals. (scienceopen.com)

Sites involved by LCV included the colorectum (2 cases), colorectum and terminal ileum, terminal ileum only, duodenum, and jejunum (1 case each). (stanfordchildrens.org)

The junctional proteins ZO-1 and p120 redistribute in a dotted circle peripheral to the brush border, in the absence of cell-cell contacts. (nih.gov)
In contrast to current thinking on polarization of simple epithelia, these cells can fully polarize in the absence of junctional cell-cell contacts. (nih.gov)

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. (thermofisher.com)

Anatomy12

Organisms7

Diseases5

Chemicals and Drugs20

Analytical, Diagnostic and Therapeutic Techniques and Equipment8

Phenomena and Processes29

Information Science3

Health Care3

Geographicals2

Induce5

Kinase4

MRNA2

Secretion8

Humans5

Intracellular2

Peptides obtained1

Plasma membrane2

IECs1

Epithelial cell3

Anti-inflammatory activity1

Digestion5

Interactions4

Analyses4

Colorectal cancer cells2

Colon9

Human8

Genes1

Macrophages3

Lipids3

Basolateral1

Hydrolysis3

Receptor1

Membrane4

Tight junctions1

Pathogen1

Viability5

Inflammatory2

Permeability1

Kinases1

Cellular4

Expression5

Immune response1

Strains1

Apical2

Abundant2

Intestinal cells1

Proliferation1

Nucleus1

Extracts2

Interaction1

Flow cytometry1

Tissues2

Biol1

Mucus2

Tissue1

Duodenum1

Junctional2

Antibody1