• basement membrane
  • As an integral member of the filtration barrier in the kidney glomerulus, the podocyte is in a unique geographical position: It is exposed to chemical signals from the urinary space (Bowman's capsule), it receives and transmits chemical and mechanical signals to/from the glomerular basement membrane upon which it elaborates, and it receives chemical and mechanical signals from the vascular space with which it also communicates. (nih.gov)
  • Podocyte loss leads to a denuded basement membrane, adhesions, misfiltration of primary urine, and in turn to inflammation and scarring. (physiology.org)
  • urine
  • Especially the detection of podocytes in the urine could be a valuable noninvasive method to get information about the disease activity or disease type and could be used as a follow-up after a biopsy in an outpatient setting. (physiology.org)
  • This review summarizes the available information on marker proteins that have been successfully used in the diagnostic of "podocytes" in the urine. (physiology.org)
  • Because podocyte loss seems to be an important component of disease progression in glomerular diseases, it seems reasonable to look for podocytes in the urine as a possible marker of disease activity or adverse prognosis. (physiology.org)
  • Podocalyxin (PDX) is probably the most frequently used marker protein for podocyte diagnostic in the urine. (physiology.org)
  • Patients in the remission group received prednisone (2 mg/kg/d) for 4 w, with negative urine protein at the end of therapy, suggesting complete remission. (alliedacademies.org)
  • When the amount of proteins excreted in the urine exceeds the liver's ability to compensate, fewer proteins are detected in the blood - in particular albumin, which makes up the majority of circulating proteins. (wikipedia.org)
  • molecular
  • In conclusion, the study indicated that loss of WWC1 may contribute to podocyte apoptosis by inducing nuclear relocation of dendrin protein, which provided novel insight into the molecular events in podocyte apoptosis. (spandidos-publications.com)
  • Previous genetic studies have highlighted the molecular makeup of SD and podocyte FP ( 5 - 6 ). (spandidos-publications.com)
  • SD is comprised by molecular proteins functioning as 'protein gatekeepers' in the junctional domain of the podocyte. (spandidos-publications.com)
  • Despite of these results, the precise molecular mechanism by which dendrin regulates function of podocyte remains to be fully understood. (spandidos-publications.com)
  • RESEARCH DESIGN AND METHODS We used publicly available "omics" data to develop a molecular process model of CKD in diabetes and identified a representative parsimonious set of nine molecular biomarkers: chitinase 3-like protein 1, growth hormone 1, hepatocyte growth factor, matrix metalloproteinase (MMP) 2, MMP7, MMP8, MMP13, tyrosine kinase, and tumor necrosis factor receptor-1. (diabetesjournals.org)
  • The encoded protein has 926 amino acids with a predicted molecular weight of 105,123 Daltons. (wikipedia.org)
  • novel
  • Due to the fact that CKD is a worldwide chronic disease and annually millions die of cardiovascular or renal complications directly due to CKD, exploring factors intervening in the pathogenesis of podocyte injury is necessary in the development of novel strategies for CKD clinical therapy. (spandidos-publications.com)
  • In yeast two hybrid screens with the human isoform of dendrin, Kremerskothen et al ( 20 ) isolated a cDNA coding for a novel protein, WWC1 (also termed KIBRA) ( 20 ). (spandidos-publications.com)
  • Conclusions Schip1 is a novel glomerular protein predominantly expressed in podocytes, necessary for the zebrafish pronephros development and function. (diva-portal.org)
  • Kinase
  • The biochemical lesion appears to be in the Kinase, Endopeptidase and Other Proteins of small Size (KEOPS)/Endopeptidase-like and Kinase associated to transcribed Chromatin (EKC) (KEOPS/EKC) complex. (wikipedia.org)
  • Dendrin is a protein kinase substrate that is composed of multiple consensus sites for phosphorylation by protein kinase C, casein kinase 11, CAMP-dependent and proline-dependent kinases, and tyrosine kinase. (wikipedia.org)
  • This gene is also known by several other names: CMG 2 (CAMGUK protein 2), calcium/calmodulin-dependent serine protein kinase 3 and membrane-associated guanylate kinase 2. (wikipedia.org)
  • Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 is an enzyme that in humans is encoded by the MAGI1 gene. (wikipedia.org)
  • The protein encoded by this gene is a member of the membrane-associated guanylate kinase homologue (MAGUK) family. (wikipedia.org)
  • Membrane Proteins
  • Podocyte plasma membrane proteins and a canonical pattern of injury. (nih.gov)
  • Shown is an (incomplete) list of membrane proteins that have been implicated in the regulation of podocyte function in health and disease. (nih.gov)
  • The protein directly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin binding sites, SH3 domains, and a proline-rich region containing binding sites for SH3 domains. (wikipedia.org)
  • binds
  • It interacts with the transcription factor TBR1 and binds to several cell-surface proteins including amyloid precursor protein, neurexins and syndecans. (wikipedia.org)
  • zinc fingers and homeoboxes
  • In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family. (wikipedia.org)
  • The encoded Protein contains two C2H2-type zinc fingers and five Homeodomains and forms a Dimer with itself or with Zinc fingers and homeoboxes family member 1. (wikipedia.org)
  • mice
  • Here, we employed podocyte-specific Cre-lox technology and found that mice with deletion of Rac1 display normal podocyte morphology without glomerular dysfunction well into adulthood.Cdc42 is necessary for the maintenance of podocyte structure and function, but Rac1 is entirely dispensable in physiological steady state.However, Rac1 has either beneficial or deleterious effects depending on the context of podocyte impairment. (nih.gov)
  • Here, we employed podocyte-specific Cre-lox technology and found that mice with deletion of Rac1 display normal podocyte morphology without glomerular dysfunction well into adulthood. (nih.gov)
  • Adriamycin nephrotoxicity and subtotal nephrectomy (SNx) studies indicated that deletion of the histone methylating enzyme EZH2 from podocytes decreased H3K27me3 levels and sensitized mice to glomerular disease. (jci.org)
  • Decreasing H3K27me3 levels in podocytes renders mice susceptible to glomerular injury. (jci.org)
  • C ) Immunoblotting for EZH2 in primary cultured podocytes from EZH2 Ctrl ( n = 3) and EZH2 podKO ( n = 3) mice. (jci.org)
  • interacts
  • Dendrin, another newly identified SD complex, interacts with CD2AP, and loss of CD2AP could drive dendrin protein from the SD to the nucleus ( 16 ). (spandidos-publications.com)
  • In the Nucleus, the dimerized protein interacts with the A subunit of the ubiquitous transcription factor nuclear factor-Y and may function as a transcriptional repressor. (wikipedia.org)
  • intracellular
  • As with every cell, the ability of the podocyte to receive signals from the surrounding environment and to translate them to the intracellular milieu is dependent largely on molecules residing on the cell membrane. (nih.gov)
  • This gene encodes a receptor-type protein tyrosine phosphatase containing a single intracellular catalytic domain with a characteristic signature motif. (wikipedia.org)
  • clinical
  • CONCLUSIONS A small set of serum protein biomarkers identified using a systems biology approach, combined with clinical variables, enhances the prediction of renal function loss over a wide range of baseline eGFR values in patients with type 2 diabetes and CKD. (diabetesjournals.org)
  • A wide spectrum of clinical features have been associated with ANKRD1/CARP protein. (wikipedia.org)
  • adriamycin
  • Conversely, inhibition of the Jumonji C domain-containing demethylases Jmjd3 and UTX increased the H3K27me3 content of podocytes and attenuated glomerular disease in adriamycin nephrotoxicity, SNx, and diabetes. (jci.org)
  • CARP, also known as Cardiac adriamycin-responsive protein or Cardiac ankyrin repeat protein is a protein that in humans is encoded by the ANKRD1 gene. (wikipedia.org)
  • signaling molecules
  • In this review, we take a membrane biologist's view of the podocyte, examining the many membrane receptors, channels, and other signaling molecules that have been implicated in podocyte biology. (nih.gov)
  • membranes
  • Certain Strongylocentrotus purpuratus sperm mitochondrial proteins co-purify with low density detergent-insoluble membranes and are PKA or PKC-substrates possibly involved in sperm motility regulation. (semanticscholar.org)