Proteins mutants gene deletion. Medical search. Frequent questions

in 2016, a tenth type was defined based on mutations in the AP3D1 gene. (medscape.com)
Ironically, as both papers point out, it might be the case that the DNA repair defects caused by mutation of BRCA2 increase the likelihood of mutations that lead to the deletion of the 6174delT frameshift and drug resistance. (nature.com)
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. (thebiogrid.org)
Missense mutations in the splicing factor gene PRPF31 cause a dominant form of retinitis pigmentosa (RP11) with reduced penetrance. (molvis.org)
Missense mutations in PRPF31 have previously been shown to cause reduced protein solubility, suggesting insufficiency of functional protein as the disease mechanism. (molvis.org)
The RP11 missense mutations exert their pathology mainly via a mechanism based on protein insufficiency due to protein insolubility, but there is also a minor direct negative effect on function. (molvis.org)
Studies conducted on PRPF31 mRNA levels in lymphoblast cell lines isolated from family members with either deletion or splice site mutations indicate that penetrance requires the co-inheritance of a low expressing wild-type (WT) allele alongside the mutant allele [ 13 , 14 ], whereas the presence of a high expressing WT allele is protective. (molvis.org)
This paper describes the construction of 10 mutant HSV-1 viruses with deletion and insertion mutations in Vmw110. (microbiologyresearch.org)
At low multiplicity, mutations in Vmw110 reduced viral gene expression in the least permissive cell type. (microbiologyresearch.org)
Mutations involving the SRY-related gene SOX8 are associated with a spectrum of human reproductive anomalies. (dundee.ac.uk)
Dive into the research topics of 'Mutations involving the SRY-related gene SOX8 are associated with a spectrum of human reproductive anomalies. (dundee.ac.uk)
Mutations in this gene have been associated with Peutz-Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. (cancerindex.org)
Scope includes mutations and abnormal protein expression. (cancerindex.org)
We have identified mutations in four of those 17 deafness genes in Japanese families. (nih.gov)
Myelodysplastic syndromes with 20q deletion: incidence, prognostic value and impact on response to azacitidine of ASXL1 chromosomal deletion and genetic mutations. (nih.gov)
Spliceosomal gene aberrations are rare, coexist with oncogenic mutations, and are unlikely to exert a driver effect in childhood MDS and JMML. (nih.gov)
Clinically Significant CUX1 Mutations Are Frequently Subclonal and Common in Myeloid Disorders With a High Number of Co-mutated Genes and Dysplastic Features. (nih.gov)
Scientists in recent decades have linked thousands of gene mutations to human diseases, yet many of the genes in question code for proteins of unknown function. (news-medical.net)
Our data suggest that, although the talpid2 and talpid3 mutations affect a common ciliogenesis pathway, they are caused by mutations in different ciliary proteins that result in differences in craniofacial phenotype. (usda.gov)
At least 23 mutations in the AMELX gene have been identified in people with an X-linked form of a disorder of tooth development called amelogenesis imperfecta. (medlineplus.gov)
X-linked disorders are caused by mutations in genes on the X chromosome. (medlineplus.gov)
Some AMELX gene mutations lead to the production of an abnormal version of the amelogenin protein that can interfere with the formation and organization of enamel crystals. (medlineplus.gov)
Other AMELX gene mutations prevent one copy of the gene from producing any amelogenin protein at all. (medlineplus.gov)
Seven novel mutations in the adenosine deaminase (ADA) gene in patients with severe and delayed onset combined immunodeficiency: G74C, V129M, G140E, R149W, Q199P, 462delG, and E337del. (lu.se)
Better understanding of new mutations and the wide range of possible phenotypes led to the development of a new nomenclature proposal, based on the gene and inheritance pattern. (medscape.com)
[ 6 ] However, the gene mutations responsible for the different forms of CMT1 are clearly myelin genes. (medscape.com)

It has been found that children with Rolandic epilepsy have a mutation of gene coding for the Elongator Protein Complex 4, which is involved in transcription and tRNA modification. (wikipedia.org)
HPS-2 is caused by a mutation in the gene encoding the beta-3A subunit of the heterotetrameric AP3 complex ( AP3BA ), which assists in the vesicle formation from the trans-Golgi network or late endosome. (medscape.com)
The TP53 gene mutation is the most common gene alteration in many tumors, including esophageal cancer. (spandidos-publications.com)
Although the biological significance of TP53 gene mutation is well characterized, its clinical significance in esophageal cancer remains controversial, especially as a prognostic biomarker. (spandidos-publications.com)
Therefore, the overexpression of p53 does not always indicate a mutation in the TP53 gene. (spandidos-publications.com)
Similarly, TP53 mutation does not always lead to the accumulation of p53 protein in cells. (spandidos-publications.com)
This cell line expresses a mutant BRCA2 with a frameshift mutation (6174delT) that results in a truncated form of the protein that lacks the carboxyl terminus and cannot bind RAD51. (nature.com)
Further examination indicated that some had increased copies of the BRCA2 gene and intragenic deletions of varying size that deleted the frameshift mutation and restored expression of the C-terminal portion of the protein. (nature.com)
Each deletion mutation alone has only a moderate effect on survival after exposure to UV radiation. (wikigenes.org)
HPS-2 is caused by a mutation in the gene encoding the beta-3A subunit of the heterotetrameric AP3 complex (ADTB3A), which resides on chromosome 5. (medscape.com)
Scientists at The Scripps Research Institute (TSRI) have discovered that a gene mutation linked to hereditary spastic paraplegia, a disabling neurological disorder, interferes with the normal breakdown of triglyceride fat molecules in the brain. (news-medical.net)
We often need to understand the protein function that is disrupted by a gene mutation, if we're going to understand the mechanistic basis for the disease and move towards developing a therapy, and that is what we've tried to do here,' said Benjamin F. Cravatt, professor and chair of TSRI's Department of Chemical Physiology. (news-medical.net)
In this submission, using a genomics and molecular biology, the causative gene and likely mutation for talpid 2, characterized by polydactyly, was identified. (usda.gov)
Kida M, Sakiyama Y, Matsuda A, Takabayashi S, Ochi H, Sekiguchi H, Minamitake S, Ariga T. A novel missense mutation (p.P52R) in amelogenin gene causing X-linked amelogenesis imperfecta. (medlineplus.gov)
A novel mutation in the ADA gene causing severe combined immunodeficiency in an Arab patient: a case report. (lu.se)
Carrier frequency of a nonsense mutation in the adenosine deaminase (ADA) gene implies a high incidence of ADA-deficient severe combined immunodeficiency (SCID) in Somalia and a single, common haplotype indicates common ancestry. (lu.se)
This disease, previously referred to as MCKD type 1, is due to a mutation in the variable-number tandem repeat region of the MUC1 (Mucin 1) gene. (medscape.com)
The frameshift mutation results in the formation of a truncated protein which cannot fold properly, promoting aggregation, and subsequent deposition in tubular cells. (medscape.com)
The reason for the observed defective assembly in E. coli is probably not some unidentified mutation in the plasmid DNA because the same plasmid preparation complemented a B. subtilis sdhCAB deletion strain [7]. (lu.se)

The psbH deletion mutants gave a PSII minus phenotype and were unable to grow photoautotrophically. (ucl.ac.uk)
Carbon source does not drastically affect the growth phenotype of mutants tested. (uwm.edu)
Conversely, both the deletion of rpsF and the deletion of rimK, which encode S6 and S6 modification enzymes, respectively, showed an HU-resistant phenotype. (singerinstruments.com)
We set out to determine the etiology of the craniofacial phenotype of this mutant. (usda.gov)
Through additional fine mapping and whole-genome sequencing, we determined that the talpid2 phenotype was linked to a 1.4 Mb region on GGA1q that contained the gene encoding the ciliary protein C2CD3. (usda.gov)
Finally, we identified a 19 bp deletion in talpid2 C2CD3 that produces a premature stop codon, and thus a truncated protein, as the likely causal allele for the phenotype. (usda.gov)

Here, we use a reporter gene-based screen in Saccharomyces cerevisiae for the discovery of antifungal inhibitors of GPI-anchoring of proteins, and identify the oligocyclopropyl-containing natural product jawsamycin (FR-900848) as a potent hit. (nature.com)
We generated a global genetic interaction network for Saccharomyces cerevisiae, constructing more than 23 million double mutants, identifying about 550,000 negative and about 350,000 positive genetic interactions. (thebiogrid.org)
Here we apply the system to the determination of gene function using deletion mutants of yeast (Saccharomyces cerevisiae) and auxotrophic growth experiments. (aber.ac.uk)

This comprehensive network maps genetic interactions for essential gene pairs, highlighting essential genes as densely connected hubs. (thebiogrid.org)
TIPI-gTOW is also useful for analyzing genetic interactions of essential genes whose deletion mutants cannot be obtained. (biomedcentral.com)

The human gene can partially complement sensitivity phenotypes of yeast ELP4 deletion mutants. (wikipedia.org)
To identify near complete sets of genes required for the cell cycle and cell shape, we have visually screened a genome-wide gene deletion library of 4843 fission yeast deletion mutants (95.7% of total protein encoding genes) for their effects on these processes. (uzh.ch)
Zap1p activates the transcription of its target genes in zinc-limited but not in zinc-replete yeast cells. (biomedcentral.com)
In a complementary study, Foury and Talibi [ 4 ] identified genes upregulated in a yfh1 mutant strain of yeast. (biomedcentral.com)
KlCdc42, another protein of the same small GTPase subfamily, participates in yeast bud site selection and was found to largely complement the morphological defects of Klrho5 deletions, indicating overlapping functions. (uni-osnabrueck.de)

Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only one has been determined. (wikipedia.org)
The impaired function of specific organelles indicates that the causative genes encode protein complexes that regulate vesicle trafficking in the endolysosomal system including AP-3, BLOC-1, BLOC-2, and BLOC-3. (medscape.com)
The impaired function of specific organelles indicates that the causative genes encode proteins operative in the formation of lysosomes and vesicles. (medscape.com)
Deletion of genes that encode these proteins results in an aerobic growth deficiency. (uwm.edu)

In this way, bacteria gain novel genetic information, for example metabolic traits, pathogenicity determinants and resistance genes as a driving force for bacterial adaptation and evolution. (elifesciences.org)
The polyphagous mutants, Bt and spli were analyzed to understand the genetic basis of mulberry feeding in Bombyx. (nii.ac.jp)
Genetic mapping anchored the Lig 1 gene onto 2H within an '300-kb region. (usda.gov)
In this study, we used the TEV protease-mediated induction of protein instability (TIPI) in combination with the genetic Tug-of-War (gTOW) to develop a method to measure the lower limit of protein level. (biomedcentral.com)
TIPI-gTOW is useful for estimating changes in the lower limit of a protein under different conditions, such as different genetic backgrounds and environments. (biomedcentral.com)
It is notable that both zrt3 and zrc1 mutants grow normally under zinc-replete or zinc-limiting conditions and, thus, would have been very difficult to identify using classical genetic approaches. (biomedcentral.com)
Developing predictive models of multi-protein genetic systems to understand and optimize their behavior remains a combinatorial challenge, particularly when measurement throughput is limited. (omictools.com)
We report 8 patients from 7 Jordanian families, 6 of whom underwent genetic testing and were found to have a 12 bp (155-166 del) deletion within the tubulin-specific chaperone E (TBCE gene) in exon 3 at 1q42-43. (who.int)
Now a large and ever increasing number of genetic subtypes has been described, and major advances in molecular and cellular biology have clarified the understanding of the role of different proteins in the physiology of peripheral nerve conduction in health and in disease. (medscape.com)

The Type VI Secretion System (T6SS) is a multiprotein device that has emerged as an important fitness and virulence factor for many Gram-negative bacteria through the injection of effector proteins into prokaryotic or eukaryotic cells via a contractile mechanism. (frontiersin.org)
Of note, the genes encoding these effectors and immunity proteins are widely distributed in Salmonella genomes, suggesting a relevant role in interbacterial competition and virulence. (frontiersin.org)
Deletion of the SOWgp gene by using a targeted gene replacement strategy resulted in partial loss of the ability of intact spherules to bind to ECM proteins and a significant reduction in virulence of the mutant strain. (kenyon.edu)
The parasitic cell surface glycoprotein encoded by the SOWgp gene appears to function as an adhesion and contributes to the virulence of C. immitis. (kenyon.edu)
These genes code for proteins that may be involved in sensing and correcting the misfolding of envelope proteins critical for virulence. (ubc.ca)
Genome-wide transcript analysis showed that B. anthracis undergoes considerable changes in gene expression during growth in iron-depleted media, including the regulation of known and candidate virulence factors. (omictools.com)

Tumor protein 53 ( TP53 ) is a recognized tumor suppressor gene located on chromosome 17q13.1. (spandidos-publications.com)
The AMELX gene, which is located on the X chromosome, makes almost all of the body's amelogenin. (medlineplus.gov)
The copy of the amelogenin gene on the Y chromosome, AMELY , makes very little amelogenin and is not needed for enamel formation. (medlineplus.gov)
The normal AMELY gene on the Y chromosome does not provide enough amelogenin to compensate. (medlineplus.gov)
Females who inherit one altered copy of the AMELX gene are less severely affected than males because they have a normal copy of the gene on the other X chromosome to produce amelogenin. (medlineplus.gov)
It is linked to the TBCE gene on chromosome 1q42-43 which encodes for the tubulin-specific chaperone E protein [2-4]. (who.int)
The locus at chromosome 1q21 was identified by linkage mapping in 1998, but the gene has only recently been discovered due to difficulty with sequencing this highly repetitive region and was previously missed using next-generation sequencing. (medscape.com)

This thesis will discuss mutants created in the subunits PSII-H and PSII-N, the isolation and sequencing of the psbW gene in C.reinhardtii and the characterisation of the conserved gene ycf9. (ucl.ac.uk)
Mutant subunits as described previously (16). (mingsheng88.org)
The reconstitution of core enzyme was carried out using the purified and subunits, and either wild-type or mutant subunits according to the standard procedure (16). (mingsheng88.org)
First, in silico analyses including sequence alignments were employed to identify the homologues of RHO5 and the genes encoding the subunits of its dimeric GEF, DCK1 and LMO1, in the K. lactis genome. (uni-osnabrueck.de)
If the membrane-anchor is missing or defective, Fp and Ip subunits accumulate as soluble proteins in the cytoplasm. (lu.se)

This gene encodes a component of the six subunit elongator complex, a histone acetyltransferase complex that associates directly with RNA polymerase II during transcriptional elongation. (wikipedia.org)
Photosystem two is a multi subunit protein complex which carries out a photochemical reaction producing reduced plastoquinone and molecular oxygen as the products. (ucl.ac.uk)
The final chapter discusses the nuclear encoded subunit of the PSII core, PSII-W. We report the isolation and partial sequencing of the gene for this protein in C. reinhardtii. (ucl.ac.uk)
In this work, we made use of the inducible SDHD-ESR mouse, a conditional mutant in the SdhD gene, which encodes the small subunit of MCII, and that acts as a tumor suppressor gene in humans. (ed.ac.uk)
Their technique involved the disruption of selected genes with counter-effects on targeted protein production and simultaneous integration of glucoamylase landing sites into the disrupted gene locus such as protease regulator (prtT) in an ATP-dependent DNA helicase II subunit 1 (kusA or ku70)-deletion strain. (bvsalud.org)

For example, viral infection, stress and the regulation of other proteins can also change the aggregation of p53 protein ( 20 ). (spandidos-publications.com)
Intrinsically disordered protein mesophases and their regulation are at the center of cellular nano organisation that guide development and cellular dynamics. (infn.it)
The regulation of transcription of viral and cellular genes by herpesvirus immediate-early gene products. (microbiologyresearch.org)
Since inhibitors of protein synthesis are known to activate cellular signal transduction pathways, our findings demonstrate new mechanisms for the regulation of HSV-1 IE gene expression which may be important during latency and reactivation. (microbiologyresearch.org)
A novel function for KlRho5 was discovered in the regulation of cytokinesis, as deletion mutants displayed protruding bud scars, aberrant primary septa and a thickened cell wall. (uni-osnabrueck.de)

Importantly, barcodes allow us to screen mutants for specific phenotypes and we are excited to think about all the new possibilities this creates. (sanger.ac.uk)
Upon cloning and heterologous expression, KlRHO5 complemented the phenotypes of a Scrho5 deletion. (uni-osnabrueck.de)

[ 6 ] Cells deficient in the HPS5 protein maintain early-stage melanosome formation and Pmel-17 trafficking. (medscape.com)
We have analysed the effect of cycloheximide on IE gene expression with HSV-1 mutants deficient in the production of functional levels of the three major transactivators, the virion protein (VP16) and two IE proteins (ICP0 and ICP4). (microbiologyresearch.org)
Loss-of-function mutant of Lig 1 obtained by fast neutron-mutagenesis is deficient in the formation of the ligule and auricle, resulting in smaller leaf angles. (usda.gov)
This work analyzes aerobic growth of mutants deficient in terminal oxidase complexes and regulatory proteins when exposed to different sole carbon sources in basal medium. (uwm.edu)
Mutants deficient in CRP and ArcA demonstrated slight aerobic growth deficiencies. (uwm.edu)

While some effector proteins specifically target bacterial or eukaryotic cells, others can target both types of cells (trans-kingdom effectors). (frontiersin.org)
In addition, there is limited information regarding the repertoire of effector proteins encoded within T6SS SPI-6 and T6SS SPI-19 gene clusters in S . Dublin. (frontiersin.org)
Although dozens of proteins act downstream of these GTPases, a comparison of effector proteins from evolutionarily diverse organisms suggests that six groups of proteins serve as the core machinery for signaling from Cdc42 and Rac. (biomedcentral.com)

She determined that a subset of the genes in the regulon were differentially expressed in the absence of Cpx, and by systematically deleting each of these genes and observing the level of infection in mice, discovered that the deletion of two genes, degP and dsbA, resulted in significantly attenuated infection by C. rodentium . (ubc.ca)
Cravatt's team compared the tissues of the no-DDHD2 mice to the tissues of mice with normal versions of the gene, and also found that the mutant mice had much higher levels of a type of fat molecule, principally in the brain. (news-medical.net)

DNA sequencing indicated that a genomic deletion of 20-kb in the mutant occurred to the gene model HORVU.MOREX.r3.2HG0202650, which encodes a putative SQUAMOSA promoter-binding protein-like (SPL) transcription factor. (usda.gov)
Two genes encoding putative internalin proteins were chosen for further study. (omictools.com)

BRCA2, along with the recombinase RAD51 , is involved in the repair of DNA double-strand breaks through homologous recombination (HR). Loss of this pathway makes BRCA2-mutant tumours sensitive to DNA cross-linking agents, such as the platinum-based drugs, and also to inhibition of poly (ADP-ribose) polymerase 1 (PARP1), an enzyme involved in DNA repair. (nature.com)
Furthermore, sequence homology between mammalian and fungal genes in the GPI pathway show modest conservation raising the chances of finding fungal-selective molecules with good therapeutic index 8 . (nature.com)
Based on their structures, the degradation pathway of 17ß-estradiol mediated by A. niger RG13B1 involved hydroxylation, oxidation, methylation, acetylation, and dehydrogenation, especially infrequent lactylation, and the key degradation enzymes were found in the gene cluster of A. niger. (bvsalud.org)
We built and tested a detailed logical model (involving genes, proteins and metabolites) of the aromatic amino acid synthesis pathway. (aber.ac.uk)

in an operon with an upstream PurR/LacI-type transcriptional regulator gene, named amlR ( ACSP50_2475 ), and a gene downstream ( ACSP50_2473 ) encoding a GGDEF-EAL-domain-containing protein putatively involved in c-di-GMP signaling. (frontiersin.org)
Transcription of the aml operon is significantly repressed in the wild type when growing on glucose and repression is absent in an Δ amlR deletion mutant. (frontiersin.org)
Although AmlR apparently is a local transcriptional regulator of the aml operon, the Δ amlR strain shows severe growth inhibitions on glucose and - concomitantly - differential transcription of several genes of various functional classes. (frontiersin.org)
The first chapter discusses the creation of a series of mutants in genes of the psbB operon. (ucl.ac.uk)
However, the E. coli cells did not show increased succinate dehydrogenase activity nor did the operon complement a sdhCDAB defective E. coli mutant [7]. (lu.se)

Once polyubiquitinated, soluble proteins are readily targeted to the proteasome. (rupress.org)

Thus both oxidative stress and glucose starvation caused a rapid translocation of the proteins to the mitochondria. (uni-osnabrueck.de)

Elongation protein 4 homolog (S. cerevisiae), also known as ELP4, is a protein which in humans is encoded by the ELP4 gene. (wikipedia.org)
For example, our group has examined the genes regulated by the Zap1p transcription factor in S. cerevisiae using DNA microarrays and promoter motif analysis [ 1 ] (Figure 1 ). (biomedcentral.com)

In gTOW, we clone a target gene with its native promoter into a 2 μ-based plasmid harboring leu2d . (biomedcentral.com)
If the target protein expression reaches the upper limit, i.e., essential cellular functions come to a halt when the level of protein exceeds a certain limit, the gene/plasmid copy number must decrease to below the upper limit. (biomedcentral.com)
The other five point mutants, P293A, N294A, E302A, K304A and GSK343 cost L312A, were prepared in this study by the single-strand template mutagenesis method (15) using pGEMA as a parent plasmid. (mingsheng88.org)
These mutants increased the copy number of the p15A-based plasmid and exhibited reduced basal levels of SOS response. (singerinstruments.com)

Here, we provide the first eukaryotic resource of gene deletions, which describes a near genome-wide set of genes required for the cell cycle and cell shape. (uzh.ch)
Control of eukaryotic messenger RNA synthesis by sequence-specific DNA-binding proteins. (wikidata.org)
Eukaryotic cells use the ubiquitin proteasome system to eliminate misfolded proteins from diverse subcellular compartments to maintain protein homeostasis. (rupress.org)

Having spent much of the past years designing and running a DNA engineering pipeline, we are pleased that the Plasmo GEM project has produced vectors for the deletion of almost half of all Plasmodium berghei genes. (sanger.ac.uk)
Histidine-rich protein 2 (pfhrp2) and pfhrp3 gene deletions in Plasmodium falciparum isolates from select sites in Brazil and Bolivia. (cdc.gov)

This attenuation was amplified in a double mutant strain. (omictools.com)

The recombinant polypeptide (rSOWp) was shown to bind to mammalian extracellular matrix proteins in an in vitro assay, suggesting that the parasitic cell surface antigen may function as an adhesion. (kenyon.edu)
In mammalian cells, as many as twenty types of protein have been reported to bind to activated Cdc42 [ 2 ]. (biomedcentral.com)

Specifically, the talpid 2 allele has a deletion that would result in a truncated and probably nonfunctional protein. (usda.gov)

Promoter sequence and cell type can dramatically affect the efficiency of transcriptional activation induced by herpes simplex virus type 1 and its immediate-early gene products Vmw175 and Vmw110. (microbiologyresearch.org)
Expression from the human cytomegalovirus major IE promoter, when cloned into the genome of HSV-1 mutants, was also increased by inhibition of protein synthesis. (microbiologyresearch.org)
Activation of the ICP0 promoter was observed when protein synthesis was blocked by alternative agents. (microbiologyresearch.org)
A regulatory upstream promoter element in the Drosophila hsp 70 heat-shock gene. (wikidata.org)
We also analyzed promoter expression of the two major aerobic terminal oxidase complexes in these regulatory protein deletion mutants. (uwm.edu)
To assess promoter expression over time, a fluorescent timer system was developed using a mutant mCherry that fluoresces blue after translation and matures to red. (uwm.edu)
Promoter expression did not drastically change during the growth cycle of S. oneidensis, confirming that growth deficiencies of regulatory system mutants are not due to lack of expression of terminal oxidase complexes. (uwm.edu)
UMOD risk variants identified in the above-mentioned GWAS are located in the promoter region of the gene, leading to a theory that they altered UMOD expression. (medscape.com)

By regulating filamentous actin, Cdc42 and Rac exert a profound effect on cell shape, polarity, migration, cell:cell and cell:matrix adhesion, protein traffic, and cytokinesis. (biomedcentral.com)

As a broad host range pathogen, M. phaseolina possesses a large number of pathogen-host interaction genes including those for adhesion, signal transduction, cell wall breakdown, purine biosynthesis, and potent mycotoxin patulin. (biomedcentral.com)

Further analysis of all individual genes showed that the 4 US BdCoV strains showed similar identities to both HK-BdCoVs and BWCoV in open reading frame (ORF) 1a, ORF1b, nonstructural (NS) 7, NS8, NS9, and NS10. (cdc.gov)
However, US BdCoV strains showed higher identities to HK-BdCoVs than to BWCoV only in spike (S), envelope (E), membrane (M), and NS5a instead of all remaining genes ( Appendix Table, Figure 1). (cdc.gov)
The 4 US BdCoV strains showed relatively higher identities to BWCoV than to HK-BdCoVs in NS5b (95.9% vs. 93.8%-94.0%), NS5c (98.4% vs. 97.7%-97.9%), NS6 (94.9% vs. 88.6%-88.9%), and nucleocapsid protein (97.9% vs. 96.1%-96.5%) genes. (cdc.gov)
Furthermore, AoproS8 was continuously knocked in the glaA, amyA, and aamy gene loci in A. niger to construct multi-copy expression strains. (bvsalud.org)

In many cases, the disease gene encodes a component of phototransduction or allied processes with expression confined to the retina, for example the rod visual pigment (reviewed in [ 1 ]), or peripherin-RDS [ 2 ], but not all RP genes show such a restricted pattern of expression. (molvis.org)

This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. (cancerindex.org)

Transcription from the early and late classes of the herpes simplex virus type 1 (HSV-1) promoters requires prior immediate early (IE) gene expression. (microbiologyresearch.org)
Expression from the HSV-1 IE promoters that control synthesis of ICP0 and ICP27 was, unexpectedly, stimulated by inhibition of protein synthesis. (microbiologyresearch.org)
Transcription factors interacting with herpes simplex virus a gene promoters in sensory neurons. (microbiologyresearch.org)
RESULTS: Firstly, evaluation of different promoters for sgRNA expression revealed tRNAGly15 as the most efficient, achieving a remarkable 100% gene editing efficiency. (bvsalud.org)
This difference Rabbit Polyclonal to NCoR1 in the sequences between different promoters relates to differential gene appearance and efficient legislation. (mingsheng88.org)

Lakshminarayanan R, Bromley KM, Lei YP, Snead ML, Moradian-Oldak J. Perturbed amelogenin secondary structure leads to uncontrolled aggregation in amelogenesis imperfecta mutant proteins. (medlineplus.gov)

Bioinformatic and comparative genomic analyses allowed us to identify genes encoding three candidate antibacterial effectors located within SPI-6 and two candidate effectors located within SPI-19. (frontiersin.org)

We found that a transcription factor gene (Bmacj6) was defective in the mutants. (nii.ac.jp)
My phylogenetic and transcriptomic analyses suggested that F. graminearum uses transporter proteins to acquire micronutrients from the wheat host, and that their expression is regulated by an uncharacterised transcription factor (TF). (bspp.org.uk)
Cloning of Lig 1 demonstrated that the SPL transcription factor is conserved in monocots, providing a target for gene manipulation to maximize light interception capacity in high planting density. (usda.gov)
In addition, they play important roles in gene transcription (via activation of mitogen activated protein (MAP) kinase pathways and, in higher eukaryotes, the transcription factor NFκB), generation of reactive oxygen species, apoptosis, and cell-cycle progression. (biomedcentral.com)

The purified PatE was further used for structural and functional studies, and the technique laid the foundation for elevating the overall production of various proteins or chemicals in those industrially important fungi. (bvsalud.org)

Identification of immediate early genes from herpes simplex virus that transactivate the virus thymidine kinase gene. (microbiologyresearch.org)
Herpes simplex virus type 1 (HSV-1) transcription can be arrested at the immediate early (IE) stage by continuous treatment of cells with inhibitors of protein synthesis, usually cycloheximide, from the time of infection. (microbiologyresearch.org)
Construction and characterization of a herpes simplex virus type 1 mutant unable to transinduce immediate-early gene expression. (microbiologyresearch.org)
The RR1 gene of herpes simplex virus type 1 is uniquely trans activated by ICP0 during infection. (microbiologyresearch.org)
Differential dependence of herpes simplex virus immediate-early gene expression on de novo-infected cell protein synthesis. (microbiologyresearch.org)

Fluorescence in situ hybridization (FISH) is a molecular pathological method with high sensitivity and specificity, which uses specific DNA probes to detect chromosomal aberrations, as well as gene deletion and amplification ( 21 , 22 ). (spandidos-publications.com)
Some genes responsible for sensorineural hearing impairment have been cloned during the last several years, and the underlying mechanisms causing hearing impairment have begun to be clarified with the advent of recent developments in molecular genetics. (nih.gov)
A molecular abnormality referring to the loss of at least one copy of the SRSF2 gene. (nih.gov)
It is now easy to make large numbers of mutants, each identified through its own molecular barcode. (sanger.ac.uk)

The Elongator Protein Complex (ELP) is what regulates the growth of cortical projection neurons. (wikipedia.org)
Aft1p regulates genes involved in iron metabolism, and Mac1p regulates genes involved in copper homeostasis. (biomedcentral.com)

The second is the discovery of cyanophages (viruses infecting cyanobacteria) that carry in their genomes photosynthetic genes coding for both photosystem-I and II proteins. (infn.it)

With genome or EST sequences in hand, microarrays or macroarrays can be constructed to analyze gene-expression profiles at the genome-wide level over a range of conditions. (biomedcentral.com)

A lymphocyte-specific cellular enhancer is located downstream of the joining region in immunoglobulin heavy chain genes. (wikidata.org)
Immunoglobulin gene transcription is activated by downstream sequence elements. (wikidata.org)

talpid2 is an avian autosomal recessive mutant with a myriad of congenital malformations, including polydactyly and facial clefting. (usda.gov)

first measured the half-life of endogenously tagged MOM proteins to identify substrates suitable for mechanistic characterization of this process. (rupress.org)

The fact that the two gene deletion mutants attenuated infection was not surprising, but what was surprising is that in vivo , these genes are not under the sole control of Cpx. (ubc.ca)

The aim of the present study was to investigate the association between tumor protein 53 (TP53) gene deletion and protein expression and clinical features in esophageal squamous cell carcinoma (ESCC), and to evaluate the predictive value of these two characteristics in the prognosis of ESCC. (spandidos-publications.com)
The TP53 gene plays an important role in regulating the cell cycle, apoptosis and DNA damage repair ( 8 , 9 ). (spandidos-publications.com)
Wild-type TP53 can inhibit the cell cycle and activate apoptosis-related genes that induce apoptosis and regulate cell proliferation ( 10 ). (spandidos-publications.com)
Biosynthesis of glycosylphosphatidylinositol (GPI) is required for anchoring proteins to the plasma membrane, and is essential for the integrity of the fungal cell wall. (nature.com)
GPI biosynthesis is a conserved process, required for anchoring proteins to the plasma membrane of fungi and thus essential for the integrity of the fungal cell wall in yeasts and molds 4 . (nature.com)
An intriguing aspect to the association of these genes with retinal disease is that splicing occurs in every cell of the body so the genes must have a general housekeeping function, yet the disease pathology is restricted to the rod photoreceptors of the retina. (molvis.org)
A total of 513 genes have been identified as being required for cell cycle progression, 276 of which have not been previously described as cell cycle genes. (uzh.ch)
Deletions of a further 333 genes lead to specific alterations in cell shape and another 524 genes result in generally misshapen cells. (uzh.ch)
The mutant viruses were then studied in single-step growth curve experiments, by assaying for plaques in a variety of cell types and by analysis of viral polypeptide synthesis during productive infection at high and low multiplicities. (microbiologyresearch.org)
The patterns and amounts of viral polypeptide synthesis during high multiplicity infections with mutant and wild-type viruses were similar in all cell types. (microbiologyresearch.org)
Establishment of a rat cell line inducible for the expression of human cytomegalovirus immediate early gene products by protein synthesis inhibition. (microbiologyresearch.org)
DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract. (wikidata.org)
This gene encodes the cyclin-dependent kinase inhibitor p21(WAF1/Cip1), a factor implicated in cell cycle, senescence, and cancer. (ed.ac.uk)
The two SDHD-ESR cell lines also showed accumulation of this protein. (ed.ac.uk)
Respiration-defective mutants were significantly more resistant to HU, suggesting that the generation of reactive oxygen species(ROS) contributes to cell death. (singerinstruments.com)
The addition of OH radical scavenger thiourea rescued the HU sensitivity of these mutants, suggesting that ROS generation is the direct cause of cell death. (singerinstruments.com)
Deletion mutants in either of the three genes altered their sensitivities to oxidative and cell wall stress conditions. (uni-osnabrueck.de)
Furthermore, we analyzed differential gene expression profiles related to each vaccine delivery group and found that tissue stress, T cell receptor signaling, and NFB signaling pathways were activated in all groups. (cdc.gov)

What pathways are this gene/protein implicaed in? (cancerindex.org)

Future studies will build on these findings and further delineate the role of each gene in the Cpx regulon and their collective impacts on the pathogenesis of C. rodentium . (ubc.ca)

This is not surprising given the strong evidence for interaction between myelin and axon gene expression in development and after experimental nerve lesions. (medscape.com)

Each antibacterial effector gene is located upstream of a gene encoding a hypothetic immunity protein, thus conforming an effector/immunity (E/I) module. (frontiersin.org)

Each of these proteins must have a minimal requirement level (i.e., the lower limit) to support cellular growth under each of these conditions. (biomedcentral.com)

One copy of the amelogenin gene is located on each of the sex chromosomes (the X and Y chromosomes). (medlineplus.gov)

The biosynthesis genes are encoded by the acb gene cluster, which was identified in 1999 by Stratmann et al. (frontiersin.org)

Pull-down assays between PRPF31 and PRPF6 proteins showed a stronger interaction for the mutant than wild type, suggesting a mechanism for the negative effect. (molvis.org)
This study was undertaken in order to characterize both DNA and protein elements involved in this interaction. (mingsheng88.org)

succinate dehydrogenase, Sdh) genes cause familiar pheochromocytoma/paraganglioma tumors. (ed.ac.uk)
This new and unprecedented evidence for a link between SdhD dysfunction and p21(WAF1/Cip1) will open new avenues for the study of the mechanisms that cause tumors in Sdh mutants. (ed.ac.uk)

To infect their host, human gut pathogens such as enteropathogenic E. coli (EPEC) and enterohemorrhagic E.coli (EHEC), rely on elaborate membrane-spanning systems to adhere to the epithelial lining of the gastrointestinal tract, and inject proteins into the host cells which lead to infection. (ubc.ca)
We didn't know what genes were affected by the Cpx response, and which of these genes might be important for infection," Dr. Vogt explained. (ubc.ca)
Deletion of either gene (GBAA0552 or GBAA1340) resulted in attenuation in a murine model of infection. (omictools.com)

Mutant huntingtin expression in the hypothalamus promotes ventral striatal neuropathology. (lu.se)

Phylogenetic analysis of A) complete genome, B) spike C) envelope, D) matrix, and E) nonstructural protein 5a genes of gammacoronaviruses, including 4 US BdCoVs, 37112-1 to −4 (GenBank accession nos. (cdc.gov)

Although both GLI2 and GLI3 processing were disrupted in talpid2 mutants, only GLI3 activator levels were significantly altered in the nucleus. (usda.gov)

Anatomy17

Organisms21

Diseases11

Chemicals and Drugs58

Analytical, Diagnostic and Therapeutic Techniques and Equipment40

Psychiatry and Psychology1

Phenomena and Processes118

Disciplines and Occupations3

Technology, Industry, Agriculture1

Information Science4

Health Care1

Mutations26

Mutation19

Phenotype6

Saccharomyces3

Essential genes2

Yeast5

Encode4

Genetic9

Virulence6

Chromosome7

Subunits5

Subunit5

Regulation5

Phenotypes2

Deficient5

Effector proteins3

Mice2

Putative2

Pathway4

Operon5

Soluble proteins1

Mitochondria1

Cerevisiae2

Plasmid4

Eukaryotic3

Plasmodium2

Strain1

Mammalian2

Allele1

Promoter8

Cytokinesis1

Adhesion1

Strains4

Encodes a component1

Tumor suppressor1

Promoters5

Aggregation1

Effectors1

Transcription factor4

Functional1

Herpes simpl5

Molecular4

Regulates2

Genomes1

Genome-wide1

Downstream2

Autosomal recessive1

Characterization1

Vivo1

Cell18

Pathways1

Pathogenesis1

Lesions1

Immunity1

Cellular1

Chromosomes1

Biosynthesis1

Interaction2

Tumors2

Infection3

Hypothalamus1

Phylogenetic1

Activator1