Proteins ligands drug screening assays antitumor. Medical search. Frequent questions

A ligand binding assay (LBA) is an assay, or an analytic procedure, which relies on the binding of ligand molecules to receptors, antibodies or other macromolecules. (wikipedia.org)
The foundations for which ligand binding assay have been built are a result of Karl Landsteiner, in 1945, and his work on immunization of animals through the production of antibodies for certain proteins. (wikipedia.org)
But in at least 50% of cases, the patients produce neutralizing antibodies, rendering therapy with these proteins ineffective. (genengnews.com)
We adopted a distinct approach aimed initially at interfering with protein-protein interactions of RAS, and other so-called undruggable targets like transcription factors, using molecular biology tools called intracellular antibodies that bind to target proteins inside cells. (cancer.gov)
The former issue was often neglected prior to initiating drug discovery programmes while the latter was considered impossible because it was purported that antibodies were unsuitable to derive compounds. (cancer.gov)
These drugs are designed to break down the protein "checkpoints" that cancer cells create to protect themselves from antibodies that seek to destroy them. (timesofisrael.com)
Here we will briefly examine how click chemistry can be used to modify biomolecules used in molecular biology techniques, particularly oligonucleotides, as well as proteins and antibodies. (biosearchtech.com)

Their analysis is critical to determining the therapeutic proteins that will fail to induce immune responses while still performing their biological function. (genengnews.com)
When loaded with immune complexes (IC), consisting of tumor antigens bound to antitumor antibody, BMDC induce powerful antitumor immunity in mice. (stanford.edu)
Tumor-specific neoantigens, which are expressed on tumor cells, can induce an effective antitumor cytotoxic T-cell response and mediate tumor regression. (wjgnet.com)
In healthy individuals, cryopyrin and apoptosis-associated speck-like protein (ASC) interact via the oligomerization of ASC to induce both apoptosis and activate nuclear factor (NF)-kappa B. (medscape.com)

I demonstrated that tumor cells secreted proteins that drove the recruitment and/or expansion of pulmonary TAMs, Tregs, and granulocytic-MDSCs (G-MDSCs), resulting in increased primary lung tumor growth and pulmonary metastasis of mammary carcinoma cells, respectively. (ubc.ca)
Abstract Circulating tumor cells (CTCs) are essential biomarkers for liquid biopsies, which are important in the early screening, prognosis, and real-time monitoring of cancer. (techscience.com)

A detection method is used to determine the presence and extent of the ligand-receptor complexes formed, and this is usually determined electrochemically or through a fluorescence detection method. (wikipedia.org)
Historically, ligand binding assay techniques were used extensively to quantify hormone or hormone receptor concentrations in plasma or in tissue. (wikipedia.org)
Radioligands are used to measure the ligand binding to receptors and should ideally have high affinity, low non-specific binding, high specific activity to detect low receptor densities, and receptor specificity. (wikipedia.org)
Here, I investigate the role of one intrinsic factor (receptor-type protein tyrosine phosphatase alpha) and one extrinsic factor (ionizing radiation) in facilitating cancer cell invasion. (ubc.ca)
Receptor-type protein tyrosine phosphatase alpha (PTPα is a widely expressed transmembrane-bound protein that has been implicated in integrin signaling, focal adhesion formation, and normal cell migration. (ubc.ca)
This switching off happens when the PD1 receptor binds to the other cells' PDL1 or PDL2 ligands (also proteins). (timesofisrael.com)
The publication describes the preclinical activity of Secarna's high-affinity bimodal LNAplus™-based ASO, IM-T9P1, that simultaneously triggers toll-like receptor 9 (TLR9) signaling and downregulates programmed cell death ligand 1 (PD-L1) expression, a protein involved in the suppression of the adaptive immune system, in one molecular entity. (izb-online.de)
The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. (cancerindex.org)
The gene product is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. (cancerindex.org)
Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function. (cancerindex.org)
Explore our comprehensive collection of recombinant Fc receptor proteins! (acrobiosystems.com)
DEC-205 (CD205), a member of the macrophage mannose receptor protein family, is the prototypic endocytic receptor of dendritic cells, whose ligands include phosphorothioated cytosine-guanosine (CpG) oligonucleotides, a motif often seen in bacterial or viral DNA. (uci.edu)
Here we describe the 3.2 Å cryo-EM structure of human DEC-205, thereby illuminating the structure of the mannose receptor protein family. (uci.edu)

These findings will assist the development of anti-tumour drugs to treat lung cancer. (oncotarget.com)
This low survival may result from the metastasis of cancer cells and arising resistance to drugs. (oncotarget.com)
Terry Rabbitts is Professor of Molecular Immunology in the Centre for Cancer Drug Discovery, Institute of Cancer Research, Sutton, London, UK. (cancer.gov)
One such biological target was RAS, where decades of attempts had failed (until very recently) to yield anti-cancer drugs directly interfering with RAS function. (cancer.gov)
These drugs may deserve to be tested for their efficacy in the control of prostate cancer using in vivo models. (researchgate.net)
Biological assays to test the cytotoxicity of the compound 1b combined with electroporation were performed to determine its potential for future medical applications in cancer treatment. (hindawi.com)
The development of platinum-drug resistance in cancer patients, the general toxicity and severe side effects of platinum drugs however required a different approach in the research of anticancer metal complexes [ 2 ]. (hindawi.com)
Aiming to exploit this effect in therapy we performed a compound screen in five breast cancer cell lines with IGF neutralising antibody xentuzumab. (nature.com)
Their current application in oncology has revolutionized cancer treatment by improving the antitumor activity of several chemotherapeutic agents. (mdpi.com)
In Chapter 2, I examined how proteins secreted by cancer cells can promote a tumor-permissive microenvironment through the recruitment of suppressive innate and adaptive immune cells to the lungs. (ubc.ca)
Mechanism-based target identification and drug discovery in cancer research. (jscimedcentral.com)
Li X, Taratula O, Taratula O, Schumann C, Minko T. LHRH-Targeted Drug Delivery Systems for Cancer Therapy. (jscimedcentral.com)
Sawa M, Masai H. Drug design with Cdc7 kinase: a potential novel cancer therapy target. (jscimedcentral.com)
Besides, recent efforts have focused on developing targeted therapies for pancreatic cancer, including drugs that target specific molecular alterations like the KRAS oncogene, which is frequently mutated in pancreatic cancer ( 9 - 11 ). (frontiersin.org)
Regardless of the therapeutic approach, the heterogeneity of pancreatic cancer cells and the complexity of its tumor microenvironment contribute to the emergence of drug resistance. (frontiersin.org)
Deregulation of Jumonji-C domain-containing protein 5 (JMJD5) has been associated with multiple facets of biological processes in cancer cells. (morulaivf.com)
Cancer cells tend to have many PDL1 and PDL2 ligands, so they can evade attack from the T cells. (timesofisrael.com)
These drugs inhibit immune-suppressive signals and reinstate cancer immunosurveillance. (izb-online.de)
This method identifies peptide ligands as well as cytoxic peptides that may prove useful in developing anti-cancer agents. (peptidesguide.com)
and (4) refining "precision medicine" for metastatic breast cancer using functional assays in patient-derived breast tumor grafts. (utah.edu)
Nanoparticles (NPs) are tailored drug delivery vectors that can selectively target huge dosages of chemotherapeutic drugs or therapeutic genes into cancer cells while leaving healthy cells alone. (pdfplayer.org)
By addressing the limitations of conventional chemotherapy, such as poor biodistribution, cancer cell drug resistance, and significant systemic adverse effects, NPs hold great promise of drastically transforming the face of oncology[1-2]. (pdfplayer.org)
and many others - see Advanced Drug Delivery Systems of Curcumin for Cancer Chemoprevention for a good overview. (blogspot.com)
Epidemiological and clinical studies have suggested that cancer could be prevented or significantly reduced by treatment with anti-oxidant and anti-inflammatory drugs, therefore, curcumin, a principal component of turmeric (a curry spice) showing strong anti-oxidant and anti-inflammatory activities, might be a potential candidate for the prevention and/or treatment of cancer and other chronic diseases. (blogspot.com)
The large global market for cancer drugs is expected to increase from $85B in 2016 to $155.6B by 2025, according to the research and consulting firm Transparency Market Research. (mainlinehealth.org)
Accordingly, they illuminated new uses for IDO1 inhibitors to enhance the antitumor efficacy of hypoxia/metabolic stress-inducing drugs in cancer and other diseases. (mainlinehealth.org)
Combination drug studies using IDO inhibitors to safely enhance the efficacy of cancer cell-targeted therapeutics, including RAS/RAF pathway inhibitors, PI3K/mTOR pathway inhibitors and hypoxia-inducing agents. (mainlinehealth.org)
Cancer research has been conducted using cultured cells as part of drug discovery testing, but conventional two-dimensional culture methods are unable to reflect the complex tumor microenvironment. (medsci.org)
In the future, it may become possible to produce tumor spheroids from tissue samples of oral cancer patients, and then apply them to drug screening and to develop individualized diagnostic and treatment methods. (medsci.org)
Molecular defects are often represented by hereditary BRCA (Breast cancer gene) mutations leading to altered DNA repair, thus presuming higher efficiency of DNA-damaging agents, such as platinum drugs and poly-ADP-ribose polymerase (PARP) inhibitors. (cttjournal.com)
Therapies tailored to specific genetic lesions and diagnostic tests that assay for their respective molecular targets are now an established part of clinical practice across various tumour types, including chronic myeloid leukaemia 1 , gastrointestinal stromal tumours and epithelial tumours, such as breast and colon cancer 2 . (ersjournals.com)
In its Phase II trial targeting advanced or metastatic bladder cancer, Roche said its atezolizumab immunotherapy drug shrank tumors in 27 percent of people who expressed medium and high levels of PD-L1, a protein that appears to help cancers evade the immune system. (e-cancer.fr)
Investors will be probing kidney cancer doctors at this weekend's meeting to see which drug they prefer. (e-cancer.fr)
From the paper: 'Carriers of pathogenic germline variants (PGV) in BRCA2 could soon be offered gastric cancer screening using gastroscopy. (cdc.gov)
In the longer term, some might even take part in lung cancer screening programs. (cdc.gov)

Therefore, Src has been considered a target molecule for drug development. (oncotarget.com)
With over 50 compounds in development and 40 disease areas being studied, diverse and unique career opportunities are available in areas from Small Molecule Drug Discovery to Nonclinical, and Translational Research. (bms.com)
Wolber G, Seidel T, Bendix F, Langer T. Molecule-pharmacophore superpositioning and pattern matching in computational drug design. (jscimedcentral.com)
however, the developing of small-molecule-based fluorescent binding ligands that are highly selective in targeting these G4-structures over other types of nucleic acids is challenging. (morulaivf.com)
The combined downregulation of PD-L1 expression in a single-molecule approach resulted in exceptional antitumor activity in different tumor models. (izb-online.de)
A ligand can be referred to as an «electron donor» that is attracted to the electron acceptor at the center of a complex, for which the ligand [ion or molecule] binds to a central metal atom and forms a complex. (peptidesguide.com)

Secarna Pharmaceuticals, an IZB-based biopharmaceutical company focusing on the discovery and development of next-generation antisense oligonucleotide (ASO) therapies to address challenging or previously undruggable targets via its LNAplus™ platform, announced the publication of striking new preclinical data demonstrating that bimodal ASOs can enable efficacious long-term antitumor immunity in tumor models compared to current immune checkpoint inhibitors. (izb-online.de)
Collectively, these data show that IM-T9P1 leads to long-lasting, adaptive antitumor immunity that can be further improved when combined with T cell-targeting immune checkpoint inhibitors and can have efficacy against large, established tumors in preclinical tumor models. (izb-online.de)
Treatment with IM-T9P1 resulted in the sustained activation of a specific type of tumor infiltrating cell, dendritic cells 3 (DC3s), which can promote antitumor immunity by supporting T cells with survival signals and enabling them to perform their effector functions. (izb-online.de)
These results demonstrate the importance of DCs in overcoming ICI resistance and achieving effective and durable antitumor responses and, in many cases, antitumor immunity. (izb-online.de)
These diseases were reclassified based on the innate immunity mechanisms and the inflammasome, a protein complex containing caspases involved in the proteolytic cleavage of interleukin (IL)-1 precursors to produce active forms of IL-1 (explaining the pathogenesis of fevers in these individuals) (see Table 1). (medscape.com)

Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus. (lookformedical.com)
These groups are compatible with biological molecules, as they do not react with the functional groups found in nucleic acids, proteins and lipids. (biosearchtech.com)

Specifically, despite the human body's endogenous receptors, hormones, and other neurotransmitters, pharmacologists utilize assays in order to create drugs that are selective, or mimic, the endogenously found cellular components. (wikipedia.org)
Such advances provide researchers with the ability not only to quantify hormones and hormone receptors, but also to contribute important pharmacological information in drug development and treatment plans. (wikipedia.org)
T-cell and CD4 receptors on the surface of T cells bind to the HLA protein/T-cell epitope complex in order to generate an immune response. (genengnews.com)
Many cancers show aberrant signalling via the insulin-like growth factor (IGF) axis, activating type 1 IGF receptors (IGF-1Rs) and variant insulin receptors (INSRs) to signal via phosphatidylinositol 3-kinase-AKT-mammalian target of rapamycin (PI3K-AKT-mTOR) and mitogen-activated protein kinase kinase-extracellular signal-regulated kinases (MEK-ERK) [ 1 ]. (nature.com)
The anti-PD1 agent given in ICI therapy prevents the binding of the ligands and receptors and boosts the body's immune response. (timesofisrael.com)
But what's more important is the functionality of the binding of the ligands and receptors. (timesofisrael.com)
The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. (cancerindex.org)

In this study, lentiviral transfection of sh‑RNA was used to knock down the level of RRS1, to detect the effect of RRS1 on cell function and to explore the specific mechanism of RRS1 affecting cell invasion and metastasis by COIP and dual‑luciferase reporter gene assays. (morulaivf.com)

Our work on intracellular antibody fragments as tools for analysis of protein complexes argued that these macromolecules could also be employed for target validation in diseases and then as guides for target-specific surrogate compounds as inhibitors. (cancer.gov)
We wondered if these difficulties could be overcome by focussing on the antibody combining site (i.e. the part of antibody interacting with antigen) to guide the derivation of chemical compounds that would be surrogates of the antibody, because the combining site is the part relevant to the protein-protein interaction between antibody and antigen. (cancer.gov)
This seemed much larger than would be expected for the range of space occupied by drug-like chemical compounds, estimated at 300 to 1000 Å2 [4, 5]. (cancer.gov)
We exploited the specific binding of the antibody fragment and were able to identify RAS-binding compounds in antibody-competition assays (illustrated in Fig.1). (cancer.gov)
This library represents a selection of drug-like compounds aimed at modulating protein-protein interaction (PPI) of β-catenin with different proteins involved in significant physiological processes. (chemdiv.com)

These include EpiScreen™, a T-cell epitope mapping technology that screens peptides for their immunostimulatory properties, and EpiScreen DC:T cell assay, which screens biologics for their immunogenicity. (genengnews.com)
There are biomarkers of activation of these neurohormonal pathways, such as the natriuretic peptides, catecholamine levels and neprilysin and various newer ones, which may be employed to better understand the mechanisms of HF drugs and also aid in defining the subgroups of patients who might benefit from specific therapies, irrespective of the degree of left ventricular dysfunction. (bvsalud.org)
Developing new techniques for screening and synthesizing peptides can result in efficient and cost-effective methods which make drugs more effective, diseases easier to cure, and lives to last longer. (peptidesguide.com)
Here huge numbers of peptides are screened so as to isolate and identify critical bioactive peptides that are used for new ways to cure diseases and cancers. (peptidesguide.com)
OBOC libraries are very powerful tools that are utilized for the discovery of cysteine-rich peptides for protein tagging as well as cysteine-rich pharmaceuticals, and more. (peptidesguide.com)

The biological activity of HTS02 is assessed by a tubulin polymerization assay. (cytoskeleton.com)
Protein markers are an essential component in biological research and drug development. (acrobiosystems.com)
The active compound curcumin is believed to have a wide range of biological effects including anti-inflammatory, antioxidant, antitumor, antibacterial, and antiviral activities, which have shown a lot of potential for use in clinical medicine. (blogspot.com)

TL238) for anti-tubulin ligand drug discovery. (cytoskeleton.com)
The latter discovery was important for us because it suggests that the antibody combining sites could generally be accessible for implementing the Abd technology approach to drug discovery. (cancer.gov)
Drug repositioning and pharmacophore identification in the discovery of hookworm MIF inhibitors. (jscimedcentral.com)
Organic chemistry in drug discovery. (jscimedcentral.com)
The role of the medicinal chemist in drug discovery--then and now. (jscimedcentral.com)
EFMC would like to remind his efforts, joint with Henk Timmerman and Han van de Waterbeemd, to launch the successful series of EFMC Short Course in Medicinal Chemistry in 2009 with a very successful course on "Improving Compound Quality: Physical Chemistry and DMPK Properties in Drug Discovery. (efmc.info)

Targeting tumor-secreted proteins, or the suppressive immune cells that are expanded in response to said proteins, led to a decrease in primary and metastatic pulmonary disease. (ubc.ca)

These data identify novel therapeutic vulnerabilities and may inform future trials of IGF inhibitory drugs. (nature.com)
Drugs containing polymeric nanoparticles (PN) have better pharmacological and therapeutic properties. (pdfplayer.org)
Curcumin can be developed as a therapeutic drug through improvement in formulation properties or delivery systems, enabling its enhanced absorption and cellular uptake. (blogspot.com)

Ligand binding assays provide a measure of the interactions that occur between two molecules, such as protein-bindings, as well as the degree of affinity (weak, strong, or no connection) for which the reactants bind together. (wikipedia.org)
The only ligand recognized to bind MST1R (RON) is Macrophage Stimulating 1 (MST1), also known as Macrophage Stimulating Protein (MSP) or Hepatocyte Growth Factor-Like Protein (HGFL). (frontiersin.org)
Proteins which bind to DNA. (lookformedical.com)
The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases. (lookformedical.com)

Typically, low molecular weight drugs are not recognized by the immune system, whereas protein molecules of much higher molecular weight invariably are. (genengnews.com)
The molecular weight of proteins were 102, 88 and 80 kDa, so they were named α, β and γ-catenin. (chemdiv.com)
Libraries in and of themselves can prove even more uniform than other methods in demonstrating full-length ligands during screening since molecular weight degeneracy can be eliminated right during the sequencing process versus during the synthesis, thus making the entirety of the technique much more simplified and less costly. (peptidesguide.com)
Whether it is for protein electrophoresis or western blot, our pre-stained protein markers help you quickly determine the molecular weight of the target protein or evaluate the transfer efficiency. (acrobiosystems.com)

Specific protein-binding measures are often used as assays in diagnostic assessments. (lookformedical.com)
Explore our series of high-quality proteins covering comprehensive diagnostic indicators in order to facilitate the in vitro diagnostic research of neurological diseases. (acrobiosystems.com)

These two ruthenium(III) complexes have an octahedral geometry and contain four in-plane chlorido ligands as well as one dimethylsulfoxido and one imidazolo (NAMI-A) or two indazolo ligands (KP1019) in trans positions. (hindawi.com)
The 48 kDa subunit, RETINOBLASTOMA-BINDING PROTEIN 4, is also a component of several other protein complexes involved in chromatin remodeling. (lookformedical.com)
It is found as a subunit of protein complexes that are in involved in the enzymatic modification of histones including the Mi2 and Sin3 histone deacetylase complexes and the polycomb repressive complex 2. (lookformedical.com)
This chapter will guide users in setting up SPR experiments in order to identify protein complexes and to assess their binding affinity and/or kinetics. (bvsalud.org)

50 years of preclinical anticancer drug screening:empirical to target-driven approaches. (jscimedcentral.com)
Pre-formed fibrils are an invaluable preclinical model for exploring pathogenesis of neurological diseases through aggregation of misfolded proteins. (acrobiosystems.com)
Preclinical proof of concept for this drug class in combination with RAS/RAF, PI3K/mTOR and hypoxia-inducing targeted therapeutics. (mainlinehealth.org)

Furthermore, pyrin interacts with proline-serine-threonine phosphatase-interacting protein (PSTPIP1), also known as CD2-binding protein 1 (CD2BP1), which is a tyrosine-phosphorylated protein involved in cytoskeletal organization and thereby involved in immunologic cellular interactions. (medscape.com)
Surface plasmon resonance (SPR) is one of the most commonly used techniques to study protein-protein interactions. (bvsalud.org)
FLIM-FRET analysis of protein-protein interactions showed that PLIN5 S155 phosphorylation regulates PLIN5 interaction with adipose triglyceride lipase at the lipid droplet, but not with α-β hydrolase domain-containing 5. (uci.edu)

This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS. (lookformedical.com)

The antihemophilic protein, Factor VIII, has been used to treat Hemophilia A patients, using either protein purified from pooled plasma or recombinant Factor VIII. (genengnews.com)
of Singapore, and Artisan Pharma Inc. , of Waltham, Mass., finalized a mutually exclusive manufacturing relationship on the process development, scale-up and manufacture of ART-123, a recombinant protein for the treatment of disseminated intravascular coagulation in sepsis. (bioworld.com)

Inhibitor of checkpoint kinase CHK1 was identified as a top screen hit. (nature.com)
The study confirmed that EPA reduced concentrations of the pro-inflammatory interleukin 1-beta in rats, and also showed phosphorylation of the stress-activated protein kinase, c-jun N-terminal kinase. (bioworld.com)
Perilipin 5 (PLIN5) is a lipid-droplet-associated protein that coordinates intracellular lipolysis in highly oxidative tissues and is thought to regulate lipid metabolism in response to phosphorylation by protein kinase A (PKA). (uci.edu)

Among them, rhodomycin A was the most efficient at reducing the activity and expression of Src in a dose-dependent manner, which was also the case for Src-associated proteins, including EGFR, STAT3, and FAK. (oncotarget.com)
Cisplatin showed concentration-dependent antitumor effects due to loss of cell adhesion and spheroid disruption in each cell line, while cetuximab exhibited antitumor effects that correlated with EGFR expression in each cell line. (medsci.org)

Cryopyrin (also called NALP3, PYPAF1, or NACHT, leucine-rich repeat [LRR], and PYD domains-containing protein 3) is a member of the APAF-1/NOD1-like protein family, which regulates apoptosis and inflammation. (medscape.com)

It has been proven that combining electroporation with chemotherapy potentiates the cytotoxicity of drugs when the drugs' efficacy is limited by its uptake in the cell [ 9 - 13 ]. (hindawi.com)

β -catenin has many binding partners that mediate a diverse set of cellular functions, and the protein probably acts as a 'hub' on which many cellular signaling networks impinge. (chemdiv.com)
A family of cellular proteins that mediate the correct assembly or disassembly of polypeptides and their associated ligands. (lookformedical.com)

Fluorescence-labelled oligonucleotides have applications as probes and primers for molecular assays such as fluorescent in situ hybridisation (FISH), quantitative PCR, sequencing and live cell imaging, which are routinely used in molecular diagnostics and drug development. (biosearchtech.com)

Billingsley ML. Druggable targets and targeted drugs: enhancing the development of new therapeutics. (jscimedcentral.com)
Unfortunately, some targets with potent oncogenic activity fall into this category, and currently little is known about how to solve this problem, which largely hampered drug research on human cancers. (techscience.com)

When these mutations were incorporated into a cellular clone producing Factor VIII, the resultant protein still performed its role in the clotting cascade without raising an anti-Factor VIII immune response. (genengnews.com)
β-Catenin is a fascinating protein with many important cellular and developmental functions. (chemdiv.com)
Furthermore, ligand-modified-ß-CD, which can endow the ligand through complexation with PRX termini, improved the cellular uptake and genome-editing effects of the optimized PRX/Cas9 RNP in target cells. (bvsalud.org)
The data on the prognostic and predictive role of the tumor molecular biomarkers, as well as on clinically used and cellular therapy approaches and developing targeted drugs are presented, and the prospects for the future research are outlined. (cttjournal.com)

Treatment options are limited at best and drug resistance is common. (frontiersin.org)
drug resistance, and significant systemic adverse effects. (pdfplayer.org)
The quantification of BBR is required in clinical medicine because long-term or excessive intake can lead to drug resistance and adverse effects. (bvsalud.org)
3 Click-ligated small-cyclic DNA and mini-duplexes are being explored in drug-DNA binding analysis and as potential therapeutics because of their higher resistance to nucleases, high thermal stability and better cell penetration. (biosearchtech.com)

As a direct result of these monumental findings, researchers have continued the advancement of ligand binding assays in many facets in the fields of biology, chemistry, and the like. (wikipedia.org)

The ubiquitin-proteasome pathway precisely controls the turnover of transcription factors in the nucleus, playing an important role in maintaining appropriate quantities of these regulatory proteins. (morulaivf.com)
A retinoblastoma-binding protein that is involved in CHROMATIN REMODELING, histone deacetylation, and repression of GENETIC TRANSCRIPTION. (lookformedical.com)

You need to know how many of the ligands can suppress the T cells. (timesofisrael.com)
Special advances are achieved in HER-positive tumors using targeted therapy with drugs which suppress the tumor cell growth factors (trastuzumab, herceptin). (cttjournal.com)

Hence, blood biomarker detection has become an efficient screening tool for the early diagnosis of OSCC. (techscience.com)

Ligand binding assays are used primarily in pharmacology for various demands. (wikipedia.org)
This document consists of a description of the epidemiology of HF, pharmacology of the drug, clinical trials, use of the drug in cases with reduced ejection fraction, mildly reduced ejection fraction and preserved ejection fraction, available literature on HF guidelines, recommendations and conclusions. (bvsalud.org)

It is a reliable and versatile technology ideal for applications in bioconjugation, probe-labelling, screening assays, and the development of pharmaceuticals. (biosearchtech.com)

These tools were initially used in RAS target validation and subsequently for isolating drug leads, guided by the intracellular antibody interaction with RAS. (cancer.gov)
The corollary is that the specific interaction interface between the antibody fragment with target protein would be mimicked by the chemical compound surrogates. (cancer.gov)
Furthermore, a novel fluorescence "light-up" sensor for assaying BBR was established based on the interaction between BBR and SiO2@NH2@CNCl NPs. (bvsalud.org)

MARV assembles and buds from the host cell plasma where MARV matrix protein (mVP40) dimers associate with anionic lipids at the plasma membrane inner leaflet and undergo a dynamic and extensive self-oligomerization into the structural matrix layer. (uci.edu)

If ER stress is a significant factor that affects the disease development, it would be important to find a drug that alters these mechanisms and UPR. (helsinki.fi)
Because of their passive and ligand- based targeting mechanisms, nanoparticles (NPs) accumulate preferentially at tumor sites.The recent utilization of nanotechnology in CC diagnosis and therapy and the development of HPV vaccinations. (pdfplayer.org)

MHC-I and MHC-II proteins are key components of the antigen presentation machinery responsible for neoantigen presentation to CD8 + and CD4 + T lymphocytes, respectively. (portlandpress.com)

We describe antitumor activities of vitamin E succinate (VES), an anti-oxidant and fenbendazole (FBZ), a commonly used veterinary anthelmintic. (researchgate.net)

There are two common strategies that are adopted for this type of experiment: Increasing the amount of radioligand added while maintaining both the constant specific activity and constant concentration of radioligand, or decreasing the specific activity of the radioligand due to the addition of an unlabeled ligand. (wikipedia.org)
His achievements in understanding the role of physiochemical properties in drug activity, more precisely in the disposition of medicines ought to be particularly recognised. (efmc.info)
Using a dual luciferase assay, we assessed the effect of JMJD5 on the transcriptional activity of the c-Myc target gene. (morulaivf.com)
Neural factors are a class of protein molecules with neurotrophic activity that can promote the survival and regeneration of nerve cells. (acrobiosystems.com)
7) nuclear protein reflecting the level of proliferative activity (Ki-67) [4, 5]. (cttjournal.com)

Some newer types are called "mix-and-measure" assays because they do not require separation of bound from unbound ligand. (wikipedia.org)
The present study found that RRS1 knockdown reduced the accumulation of ribosome protein L11 (RPL11) in the nucleolus, which then migrated to the nucleoplasm and bound to c‑Myc. (morulaivf.com)

Despite being highly expressed in several tissues and malignancies, the c-MYC protein is also continuously targeted by the ubiquitin-proteasome pathway, which can either facilitate or inhibit c-MYC degradation. (morulaivf.com)

The contribution of synthetic organic chemistry to anticancer drug development. (jscimedcentral.com)

Streptavidin is a tetrameric protein providing 4 high-affinity biotin binding sites. (acrobiosystems.com)

ER stress occurs due to accumulation of unfolded proteins. (helsinki.fi)

A 100 µg sample of HTS02 protein was separated by electrophoresis on a 12% SDS-PAGE gel and stained with Coomassie Blue. (cytoskeleton.com)

Through the development of RIA technology, researchers have been able to move beyond the use of radioactivity, and instead, use liquid- and solid-phase, competitive, and immunoradiometric assays. (wikipedia.org)
Characterisation of ER stress in PD models may be important for the current and future drug development of PD. (helsinki.fi)
Additional screens for MMTV provirus entry sites in tumors revealed several other upregulated genes that are associated with other gene development pathways such as INT2, INT3, and INT4 [4,5,6]. (chemdiv.com)
To develop new CXCR4 antagonists a rational, ligand-based approach was taken, distinct from the more commonly used development strategy. (cnr.it)
Setting the corresponding isotype control antibody to detect non-specific binding can reduce the generation of false positive results and evaluate the possible influencing factors accurately in the drug development process. (acrobiosystems.com)

2013. Brine shrimp lethality assay 'an effective prescreen': Microwave-assisted synthesis, BSL toxicity and 3DQSAR studies-based designing, docking and antitumor evaluation of potent chalcones. (cytoskeleton.com)

and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell. (lookformedical.com)
Small chromosomal proteins (approx 12-20 kD) possessing an open, unfolded structure and attached to the DNA in cell nuclei by ionic linkages. (lookformedical.com)
Proteins that control the CELL DIVISION CYCLE. (lookformedical.com)
Proteins found in the nucleus of a cell. (lookformedical.com)
Relative cell viability was assessed using MTT assays. (wjgnet.com)
Western blotting was used for protein expression analysis of the collected cell masses (spheroids), and H-E staining was used for structural observation. (medsci.org)

Although initially discovered as a retinoblastoma binding protein it has an affinity for core HISTONES and is a subunit of chromatin assembly factor-1 and polycomb repressive complex 2. (lookformedical.com)
A retinoblastoma-binding protein that has an affinity for core HISTONES. (lookformedical.com)
It will include detailed protocols for (i) immobilization of proteins with the amine coupling capture method, (ii) analyte-binding analysis, (iii) affinity/kinetics measurements, and (iv) data analysis. (bvsalud.org)

Although binding assays are simple, they fail to provide information on whether or not the compound being tested affects the target's function. (wikipedia.org)

High density arrays of extraordinarily sensitive integrated microring resonators will allow many gene and protein signatures to be simultaneously quantitated from a single patient sample. (nih.gov)
Real-time PCR and Western blot analyses were used to evaluate gene and protein expression levels. (wjgnet.com)

Lipid-specific oligomerization of the Marburg virus matrix protein VP40 is regulated by two distinct interfaces for virion assembly. (uci.edu)

Deubiquitinating proteases can remove ubiquitin chains from target proteins and rescue them from proteasomal digestion. (morulaivf.com)
The cultures were exposed to cisplatin and cetuximab and the morphological changes of spheroids over time and the expression changes of target proteins were compared. (medsci.org)

Tubulin polymerization must also be responsive to polymerization enhancers (paclitaxel) and inhibitors (nocodazole) at 5 µM drug concentration. (cytoskeleton.com)
Thus, there is a need for drug adjuvants to leverage the immunomodulatory effects of targeted drugs where IDO inhibitors are well-positioned. (mainlinehealth.org)
New uses of IDO inhibitors in combination drug therapy: PCT patent application. (mainlinehealth.org)

Library has been assembled using in house structural biology insight, molecular stimulation-modeling, virtual screening of ChemDiv's novel chemistries and medicinal chemistry filtering/ranking of the resulting hits. (chemdiv.com)

Anatomy22

Organisms15

Diseases28

Chemicals and Drugs99

Analytical, Diagnostic and Therapeutic Techniques and Equipment46

Phenomena and Processes53

Disciplines and Occupations2

Technology, Industry, Agriculture2

Information Science4

Antibodies7

Induce4

Tumor Cells2

Receptor13

Cancer35

Molecule6

Immunity5

Nucleic acids2

Receptors7

Metastasis1

Compounds5

Peptides5

Biological3

Discovery6

Metastatic1

Therapeutic3

Bind4

Molecular weight4

Diagnostic2

Complexes4

Preclinical3

Interactions3

Putative1

Recombinant2

Kinase3

EGFR2

Apoptosis1

Efficacy1

Mediate2

Fluorescent1

Targets2

Cellular4

Resistance4

Facets1

Transcription2

Suppress2

Detection1

Pharmacology2

Pharmaceuticals1

Interaction3

Lipids1

Mechanisms2

Antigen1

Anti-oxidant1

Activity5

Bound2

Pathway1

Anticancer drug1

High affinity1

Accumulation1

Electrophoresis1

Development5

Toxicity1

Cell6

Affinity3

Fail1

Gene and protein2

Distinct1

Target2

Inhibitors3

Biology1