Proteins expression patterns expressed sequence tags. Medical search. Frequent questions

According to NCBI GEO microarray expression patterns, as well as UniGene's Expressed Sequence Tag (EST) profiles, NHLRC2 is expressed at low levels throughout most human tissues. (wikipedia.org)
These include previously unknown sequence data, a collection of expressed genes, molecular markers, and a DNA microarray that can be used to study aphid gene expression. (nih.gov)
Gene expression patterns in human liver cells exposed to tetrachloroethylene and it's metabolite using microarray analysis. (cdc.gov)
The molecular mechanisms underlying important physiological processes of the species were examined through differential gene expression analysis for copepodite stage 5 (CV) and female C. finmarchicus obtained from surface and deep waters in Gulf of Maine, by using an Expressed Sequence Tag (EST) microarray. (uconn.edu)
Methods and Results: We have performed a bioinformatics screen using microarray and expressed-sequence tag databases to identify potential new CTAs. (longdom.org)
Using an Affymetrix microarray-based approach we show that the differences in gene expression between male elderly and young human wounds are almost exclusively estrogen regulated. (biomedcentral.com)
Since the major variable contributing to chronic wounds in humans is being an aged male [ 4 , 5 ], our initial approach was to compare acute wound gene expression between young and old male human subjects via Affymetrix microarray. (biomedcentral.com)
The expression patterns of the cold-responsive transcripts identified by SAGE were confirmed by microarray analysis. (scite.ai)
The recent DNA microarray technique allows large-scale quantitative gene expression analysis. (scite.ai)
This database will facilitate the design of microarray and other experiments to study screwworm gene expression on a larger scale than previously possible. (usda.gov)

Sequencing of 16 M. persicae cDNA libraries generated 26,669 expressed sequence tags (ESTs). (nih.gov)
Expressed sequenced tags (ESTs) of 1 019 randomly selected clones were analyzed and then assembled 588 unigens. (korea.ac.kr)
The more recent duplication event in particular would be expected to result in many paralogous pairs of genes differing by relatively few sequence differences, thus complicating gene identification using ESTs. (biomedcentral.com)
Among the matched sequences, ESTs were identified from tissues other than berries or from berries at different developmental stages. (biomedcentral.com)
Additional MPSS signatures not matching to known grape ESTs can extend our knowledge of the V. vinifera transcriptome, particularly when these data are used to assist in annotation of whole genome sequences from Vitis vinifera . (biomedcentral.com)

The NHL repeat is found in many eukaryotic and prokaryotic proteins, and is associated with growth factor regulators. (wikipedia.org)
Triacylglycerol (TAG) is the principal energy storage form in eukaryotic organisms and represents a promising source of biodiesel production [1]. (researchsquare.com)
This makes them suitable for the purification of proteins from eukaryotic samples, thereby extending the use of polyhistidine proteins from bacterial to more complex expression systems. (genengnews.com)
Many eukaryotic cell surface proteins are attached to the plasma membrane by glycosylphosphatidylinositol (GPI) anchors. (gla.ac.uk)

Following recognition of TLR ligands, TLRs elicit innate immunity by activating multiple intracellular signaling cascades including variable adaptor proteins and transcriptional factors. (frontiersin.org)
These tag-based technologies, which do not require a priori knowledge of genomic sequence, are well-suited for transcriptional profiling. (biomedcentral.com)
This permits the assumption that the ratio of co-transcriptional protein amounts is constant, a useful assumption when performing parameter inference (See Appendix A ). (github.io)

The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. (betalifesci.com)

Hence, it means that human testis could also play a crucial role in human speciation if these two tissues exhibit similar gene expression patterns. (karger.com)
Determining the large scale anatomy of gene expression patterns of various human tissues could draw a more convincing conclusion, and better our understanding of the correlation/inter-correlation among different tissues. (karger.com)
Among the 17 tissues, the highest similarity in gene expression patterns was between human brain and testis, based on DDD and clustering analysis. (karger.com)
Bortoluzzi S, Alessi FD, Romualdi C, Danieli GA: Differential expression of genes coding for ribosomal proteins in different human tissues. (karger.com)
Promoters that confer expression in fruit tissues are important tools for genetic engineering of fruit quality traits, yet few fruit-specific promoters have been identified, particularly for citrus fruit development. (biomedcentral.com)
Genes specifically expressed in fruit tissues were selected and their isolated promoter regions were fused with the GUSPlus reporter gene for evaluation in transgenic plants. (biomedcentral.com)
Among the five candidate citrus promoters characterized in this study, the CitSEP promoter showed a fruit-specific expression pattern, while the CitWAX and CitJuSac promoters exhibited high fruit-preferential expression with strong activity in the fruit, weak activity in floral tissues and low or undetectable activity in other tissues. (biomedcentral.com)
The CitVO1, CitUNK and PamMybA promoters, while exhibiting strong fruit-preferential expression, also showed consistent weak but detectable activity in leaves and other vegetative tissues. (biomedcentral.com)
Using specific intron-spanning primer sets, polymerase chain reaction experiments were conducted to detect gene expression initially in a range of normal tissues. (longdom.org)
Genes that showed very low expression patterns in normal tissues were then tested against a range of cancer types. (longdom.org)
Because brain tissue storage and degradation after death can affect DNA methylation and postmortem brain samples are limited , other researchers have examined methylation patterns in more readily available tissues, such as the placenta and cord blood. (spectrumnews.org)
Different tissues have different functions and thus differences in which genes are expressed. (spectrumnews.org)

Genes contributing to the similarity include ribosomal protein (RP) genes as well as genes involved in transcription, translation and cell division. (karger.com)
This antiserum gave a similar immunofluorescence pattern in T. brucei to those of an epitope-tagged ribosomal protein, QM. (gla.ac.uk)

Hom (Helicobacter outer membrane) family of OMPs in H. pylori consists of four members (HomA, B, C and D). In the H. pylori genome jhp 0870 open-reading frame (ORF) that codes for HomB outer membrane protein is associated with many stomach diseases and is a novel co-marker for peptic ulcer disease (PUD) 9 , 10 . (nature.com)
These protein subunits can assemble into virus particles without viral RNA genome in mammalian cells to study virus-cell entry 2 and to develop vaccines 3 . (nature.com)
The sequence of the human nuclear genome has been completed in draft form by an international public consortium consisting of 16 sequencing centers and associated computational facilities [ 1 ]. (biomedcentral.com)
As of late 2000, the public human sequence was primarily based on approximately 24,000 accessioned bacterial artificial chromosome (BAC) clones covering 97% of the euchromatic portion of the genome [ 6 ]. (biomedcentral.com)
Thirty percent of the genome is in finished form, including the entire sequence of chromosomes 21 and 22 [ 7 ]. (biomedcentral.com)
Recently, the consortium has distributed a working draft assembly of the entire genome that removes redundancies, orients sequence fragments and clearly indicates gaps arising from sequencing and assembly. (biomedcentral.com)
The current coverage is, however, sufficient for the Human Genome Project to enter a new phase, in which the entire sequence can be analyzed to identify genes, regulatory regions and other genomic elements and structures. (biomedcentral.com)
The assembly provides simplified descriptions of the genome, as disparate data sources such as GenBank and numerous expressed sequence tag (EST) and protein databases are unified. (biomedcentral.com)
And that pattern of methyl marks across the genome can influence which genes are active or inactive at any given time. (spectrumnews.org)
Studies of postmortem brains suggest that autistic people have different methylation patterns - not only in specific genes but across the entire genome - than non-autistic people. (spectrumnews.org)
Soybean has a large (1115 Mbp) genome that has yet to be fully sequenced. (biomedcentral.com)
The identification of potential gene sequences (pHaps) from soybean allows us to begin to get a picture of the genomic history of the organism as well as begin to observe the evolutionary fates of gene copies in this highly duplicated genome. (biomedcentral.com)
When available, the information provided by whole-genome sequencing projects provides an entry into an understanding of genome structure and evolution and gene discovery, and function. (biomedcentral.com)
We used an expressed sequence tag and 454 pyrosequencing approach to initiate a study of the genome of the New World Screwworm, Cochliomyia hominivorax (Coquerel). (usda.gov)

In silico comparison of cDNA libraries identified aphid genes with tissue-specific expression patterns, and gene expression that is induced by feeding on Nicotiana benthamiana. (nih.gov)

Functional annotation of gene sequences indicate functional classifications are not randomly distributed among gene families containing few or many genes. (biomedcentral.com)

To investigate the APP in vivo interactome in an unbiased manner, we generated mice that harbor a mouse prion protein promoter-driven cDNA encoding human APP-695 fused to a C-terminal affinity tag. (jneurosci.org)
The fact that CWD prion incubation times in transgenic mice expressing elk prion protein are consistently more rapid raises the possibility that residue 226, the sole primary structural difference between deer and elk prion protein, may be a major determinant of CWD pathogenesis. (cdc.gov)
Bioassays in Tg mice expressing deer prion protein (PrP) ( 4 ) and newly created Tg mice expressing elk PrP, demonstrated low levels of CWD prions in antler velvet. (cdc.gov)

Comparison of cDNA data from three aphid lineages identified single nucleotide polymorphisms that can be used as genetic markers and, in some cases, may represent functional differences in the protein products. (nih.gov)
In this study, a suppression subtractive hybridization cDNA library approach was used for the identification of pathogen and plant genes that were differentially expressed during infection of the susceptible cultivar BRS Pérola of Phaseolus vulgaris L. A total of 979 unigenes (430 contigs and 549 singletons) were obtained and classified according to their functional categories. (frontiersin.org)
You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. (betalifesci.com)
We will now focus on establishing an immune (i.e. serological) response within cancer patients and attempt to characterise the functional significance of the proteins within individual tumours. (longdom.org)
The PEG membrane provides high biological particles separation and prevents sample for clogging due to the strong hydration of functional polymers layer and their resistance to protein adsorption. (llnl.gov)
In addition, initial sequence analysis reveals highly ordered chromosomal landscapes associated with paralogous gene clusters and distinct functional compartments. (biomedcentral.com)
Functional complementation demonstrated that HpDGAT2A, HpDGAT2B, HpDGAT2D, and HpDGAT2E had the capacity to restore TAG synthesis in a TAG-deficient yeast strain (H1246) showing a large difference in enzymatic activity. (researchsquare.com)
Our study represents systematic work on the characterization of HpDGAT2s by integrating expression patterns, AST/TAG accumulation, functional complementation, and heterologous expression in yeast, plants, and algae. (researchsquare.com)
Functional classification of the expressed genes reveals that those involved in cellular biogenesis such as polygalacturonase, pectate lyase, and pectin methylesterase make up more than 40% of the total transcripts. (scite.ai)
Our results strongly suggest that poor accumulation of proteins that play a role in stress tolerance may be why Arabidopsis pollen is cold sensitive.The functional and biochemical features of specific cell types are determined by their particular gene expression profiles. (scite.ai)

Generally, TAG biosynthesis takes place in the endoplasmic reticulum, and TAG assembly can be divided into acyl-CoA-dependent and acyl-CoA independent pathways [10]. (researchsquare.com)
There is some overlap in fluorescence patterns between tagged QM and the endoplasmic reticulum marker protein BiP. (gla.ac.uk)

IL-17C has a very restricted expression pattern and was detected as a rare expressed sequence tag (EST) in an adult prostate and fetal kidney libraries (2). (rndsystems.com)
1997). The transcriptome can be modulated by both external and internal factors, and thereby provide not only information useful for the understanding of the basic cellular biology but also a global view of biological responses over environmental stimuli.Gene expression profiles can be obtained and compared by various methods, such as RNA-DNA hybridization measurements, subtractive hybridization, subtraction libraries, and differential display. (scite.ai)
In many organisms, such as soybean, the best source of sequence information is the existence of expressed sequence tag (EST) libraries. (biomedcentral.com)
Many EST libraries were constructed from RNA extracted from various genetic backgrounds, thus gene identification from these sources is complicated by the existence of both gene and allele sequence differences. (biomedcentral.com)
We have initiated study of the genes of the New World Screwworm, Cochliomyia hominivorax, by synthesizing and sequencing DNA libraries of expressed genes from embryos, 2nd instar larvae, adult males and adult females. (usda.gov)

It has been found in the cytosol, mitochondria, and/or peroxisomes of cells in which it is expressed. (wikipedia.org)
In our laboratory, studies have been conducted to determine changes in gene expression patterns in cultured human cells exposed to TCE and TCA. (cdc.gov)
BT-474 cells, obtained expression of the receptor has important implications for their biology from American Type Culture Collection, were maintained in RPMI 1640 and therapy (1). (lu.se)
Cells which lack ER expression at diagnosis arise from an ER compart- were harvested at 60 - 80% confluency and used as a reference in all hybrid- ment within the mammary epithelium or represent evolution from an izations. (lu.se)
This gene product stimulates the motility of tumor cells, has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. (betalifesci.com)
HomB protein contributes to the colonization and persistence of H. pylori , and the presence of homB genes affects the number of bacteria adhering to the host cells. (nature.com)
VLPs are highly immunogenic and can induce elevated titers of neutralizing antibodies, even without adjuvants, thanks to the maintenance of the native conformation of viral proteins presenting repetitive epitopes to the host immune system cells. (nature.com)
The synthetic protein is designed to be secreted outside E. coli cells, and therefore can be purified using a His-tag from the cell supernatant (thereby reducing the need to lyse the cells for purification). (llnl.gov)
Here, we show that APP can coimmunoprecipitate NEEP21 from nontransgenic mouse brain and from mammalian cells stably coexpressing both proteins. (jneurosci.org)
Fatty acid (FA) profile assays revealed that HpDGAT2A, HpDGAT2D, and HpDGAT2E, but not HpDGAT2B, preferred monounsaturated fatty acyl-CoAs (MUFAs) for TAG synthesis in yeast cells, and showed a preference for polyunsaturated fatty acyl-CoAs (PUFAs) based on their feeding strategy. (researchsquare.com)
Purification of Nucleoli From Lymphoma Cells and Solubilization of Nucleolar Proteins for 2-DE Separations - Regis Dieckmann, Yohanne Coute, Denis Hochstrasser, Jean-Jacques Diaz and Jean-Charles Sanchez 11. (nhbs.com)
It is also possible to capture the entire population of proteins in cells that bind to metals such as copper or iron, the so-called metalloproteome," adds Dr. Boronowsky. (genengnews.com)
Such global gene expression patterns can be represented by a "transcriptome", which reveals the identity and the level of expression of each expressed gene in a defined population of cells (Velculescu et al. (scite.ai)
The pattern and timing of gene expression can be determined by creating recombinant DNA with a reporter gene under the control of a cis -regulatory sequence of interest and introducing it into cells or an organism. (jove.com)
Since the gene of interest and the reporter gene have the same cis -regulatory sequence, they are expressed in the same cells and at the same time. (jove.com)
The cells expressing GFP glow green when excited by wavelengths of blue to the ultraviolet range, while those expressing RFP glow red when excited by wavelengths of 488 nm or 532 nm. (jove.com)
The cells expressing the Luc gene produce luciferase enzymes that catalyze a reaction with luciferin to produce light. (jove.com)
In the first phase, sender cells grow and accumulate synthase protein. (github.io)
This propagation ends at the third phase, when cell growth and protein production slow down such that the amplifier cells cannot produce enough synthase protein to sustain signal transmission. (github.io)
Cells in a consortium will transition between these phases at different times, compounding the spatially heterogeneity of the consortium's demographic patterning to the gene expression capacity. (github.io)
Protein quantity is also diminished through dilution as cells grow and divide. (github.io)
Sofia Elmroth and her research team primarily study microRNAs, which are molecules that influence protein levels in the cells of the body indirectly by binding to messenger RNA. (lu.se)
Sequencing of DNA gives a direct picture of our genetic make-up, while sequencing of the RNA that is naturally present in cells gives a picture of which parts of our non-coding DNA are used and in what way. (lu.se)
These proteins either arise from proteins expressed by cells at the deposition site (localized), or they precipitate systemically after production at a local site (systemic). (medscape.com)
The large proteoglycan molecules made up of protein cores, and GAG branches are secreted by cells and constitute a significant fraction of the extracellular matrix of connective tissue. (medscape.com)
check the tag ADOLESCENCE HN - 2008 BX - Nutrition in Adolescence FX - Adolescent Nutrition Physiology MH - Peritoneal Stomata UI - D054048 MN - A01.047.025.600.700 MN - A10.810 MS - Natural openings in the subdiaphragmatic lymphatic plexus in the PERITONEUM, delimited by adjacent mesothelial cells. (bvsalud.org)

Mouse IL-17C cDNA encodes a 194 amino acid (aa) residues protein with a putative 14 aa signal peptide (5). (rndsystems.com)
The sequence of the four nucleotide bases encodes DNA's information. (genome.gov)
One of the well-known reporter genes is one that encodes for a protein called green fluorescent protein - or GFP. (jove.com)
SUSA2 encodes an F-box protein Actin-Related Protein 8 (ARP8) and SUSA3 encodes the chaperone protein HSP90.3. (ubc.ca)

Of the 233 randomly selected clones, 25 were identified as differentially expressed genes between 5MT resistant and 5MT sensitive wild type plants. (korea.ac.kr)
describe a novel method to identify potential transcription factors from a list of differentially expressed genes using textual similarity between the genes and transcription factors reported in the literature [ 30 ]. (biomedcentral.com)

LLNL researchers have designed a synthetic, concatemeric bacterial expression vector that expresses a protein sequence that can be digested into a single peptide. (llnl.gov)
propose a one-dimensional diffusion-reaction model, in the form of a master equation, to analyze the non-equilibrium protein sliding kinetics along a segment of bacterial DNA [ 29 ]. (biomedcentral.com)

The MPSS dataset also revealed evidence of antisense expression not previously reported in grapes but comparable to that reported in other plant species. (biomedcentral.com)
Identical LVA-ssrA degradation tags were appended to the coding sequences of the synthase, repressor, and reporter proteins to promote equal degradation rates, represented in the model as \(\rho\) with subscripts indicating the associated model species. (github.io)
The combined production and degradation terms result in the governing equation of the model's protein species. (github.io)

AD is pathologically characterized by the deposition of pathogenic Aβ peptides that are derived from larger integral membrane proteins, termed β-amyloid precursor proteins (APPs). (jneurosci.org)
Polymorphisms that slightly vary native peptides or inflammatory processes set the stage for abnormal protein folding and amyloid fibril deposition. (medscape.com)

The protein produces a green fluorescence under ultraviolet light, which enables researchers to track its location within a cell. (jove.com)
As GFP fluorescence was shown in the touch receptor neurons, this is likely a site where β-tubulin is expressed. (jove.com)
Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. (jove.com)

Purification of APP-AT protein complexes and identification of the constituents by mass spectrometry revealed several previously reported APP-interacting proteins as well as proteins involved in synaptic maintenance. (jneurosci.org)
Cube Biotech offers PureCube resins for the purification of His-tagged proteins. (genengnews.com)

Further, biophysical experiments suggested that HomA and HomB are dimeric and most likely the cysteine residues present on surface-exposed loops participate in protein-protein interactions. (nature.com)
In polyhistidine tagging, 6 to 10 histidine residues are added recombinantly to either the protein's C- or N-terminus. (genengnews.com)
However, according to Dr. Boronowsky, when membrane proteins are being investigated, 10 or even 14 residues might be employed to overcome the masking effects of any detergent that might have been added to improve protein solubilization. (genengnews.com)
Sequence comparison of L. mexicana, yeast and human GPI8 proteins identified two potential active site cysteine residues. (gla.ac.uk)

IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2). (rndsystems.com)

Large scale gene analysis of most organisms is hampered by incomplete genomic sequences. (biomedcentral.com)

The nucleotide sequence of a fragment of the T. brucei GPI8 gene has also been obtained. (gla.ac.uk)

H. pylori attaches and colonizes to the human epithelium using some of their outer membrane proteins (OMPs). (nature.com)
Outer membrane proteins (OMPs) of H. pylori play a crucial role in the host-pathogen interaction, virulence and pathogenesis. (nature.com)
Outer membrane proteins (OMPs) are distinctive features of Gram-negative bacteria. (nature.com)
Here, we demonstrate that non-infectious SARS-CoV-2 VLPs can be successfully assembled by co-expressing three important viral proteins membrane (M), envelop (E) and nucleocapsid (N) in plants. (nature.com)
SARS-CoV-2 is composed of four essential structural proteins: the spike (S) glycoprotein, membrane (M) protein, envelope (E) protein, and nucleocapsid (N) protein, as well as multiple accessory and non-structural proteins 1 . (nature.com)
Using this tag, we prepared mild detergent lysates from transgenic mouse brain cortical membrane preparations and isolated a number of previously identified APP-interacting proteins. (jneurosci.org)
Aβ is liberated from type I integral membrane proteins, termed β-amyloid precursor proteins (APPs), by the concerted action of β-secretase (BACE1) and γ-secretase (for review, see Selkoe, 2002 ). (jneurosci.org)
In higher plants and microalgae, there are four major groups of DGATs: (1) the membrane bound form of DGAT1, (2) the membrane bound form of DGAT2 sharing low sequence similarity with DGAT1, (3) the soluble type of DGAT3, which is localized in the cytosol, and (4) the dual function of WS/DGAT, which possesses both wax ester and TAG biosynthesis activities [12-18]. (researchsquare.com)
Analysis of Membrane Proteins by Two-Dimensional Gels - Michael Fountoulakis, 15. (nhbs.com)

DAVID was used to perform the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis for the different expression genes (DEGs). (medscimonit.com)
Such EST data form a valuable foundation for the understanding of the gene composition and genomic biology of yet-to-be fully sequenced genomes [ 3 ]. (biomedcentral.com)

Yigiterhan, Ebru Unal, "Population Genetic Structure and Gene Expressions Patterns of the Copepod Calanus finmarchicus in the North Atlantic Ocean" (2011). (uconn.edu)
Use of these fruit specific/preferential promoters for genetic engineering can help with precise expression of beneficial genes and help with accurate prediction of the activity of new genes in host fruit plants. (biomedcentral.com)
Using genetic sequence data in combination with physical and behavioural measurements of promiscuity in birds, Dr Alison Wright and Professor Judith Mank report strong evidence for the role of neutral forces in sex chromosome evolution. (ucl.ac.uk)
These results (1) update the gene models of HpDGAT2s , (2) prove the TAG biosynthesis capacity of HpDGAT2s, (3) show the strong preference for MUFAs and PUFAs, and (4) offer target genes to modulate TAG biosynthesis by using genetic engineering methods. (researchsquare.com)
Further understanding of the pathways and regulatory mechanisms involved in TAG accumulation will facilitate the genetic engineering of microalgae [7-9]. (researchsquare.com)
Studies from the past decade implicate an additional layer of 'epigenetic' complexity: chemical tags called methyl groups laid on top of a person's genetic code. (spectrumnews.org)
A codon is a DNA or RNA sequence of three nucleotides (a trinucleotide) that forms a unit of genetic information encoding a particular amino acid. (genome.gov)
Autosomal dominant is a pattern of inheritance characteristic of some genetic disorders. (genome.gov)
Autosomal recessive is a pattern of inheritance characteristic of some genetic disorders. (genome.gov)
In chapter 2, by using CRISPR/Cas9 gene editing, genetic analysis and biochemical assays, I identified the differential pairings of typical NLR receptor SOC3 with atypical NLR proteins CHS1 or TN2 to guard the homeostasis of the E3 ligase SAUL1. (ubc.ca)
Co-transcribed proteins are expressed proportionally to one another, the proportionality being dependent on their genetic context and RBS sequence. (github.io)

Public attention surrounding completion of the draft human sequence has fostered the impression that we are entering a 'post-genomic' era, and that description of genes and their functions is straightforward. (biomedcentral.com)
An allele is one of two or more versions of DNA sequence (a single base or a segment of bases) at a given genomic location. (genome.gov)
Genomic sequencing of selected loci indicate that intron-like intervening sequences are numerous and are approximately 220 bp in length. (biomedcentral.com)

Native or wild-type quaternary protein structure is usually born from a single translated protein sequence with one ordered conformation with downstream protein interactions. (medscape.com)

Researchers at LLNL have developed a nanotube sensor (single-walled or multi-walled carbon nanotubes) enclosed within a highly selective lipid bilayer that can detect variations in ion transport using signal amplification generated from the disruption of protein pores across the lipid layer. (llnl.gov)
Numerous cellular proteins detect DNA damage and induce senescence , a permanent change of state characterized by morphological and gene expression changes. (massgenomics.org)
These antibodies detected recombinant protein but failed to detect protein in L. mexicana cell lysates. (gla.ac.uk)
By studying gene expression patterns of an organism, one can learn how that organism responds to its environment and thereby detect vulnerabilities in its lifecycle which can be exploited to develop control technologies. (usda.gov)
Plant intracellular nucleotide binding and leucine-rich repeat proteins (NB-LRR, NLRs) function as immune receptors to detect microbial pathogens directly or indirectly. (ubc.ca)

Up-regulation of genes involved in cell division, protein synthesis and mating were remarkable in deep females and CVs. (uconn.edu)
Each time an amino acid is added to a growing polypeptide during protein synthesis, a tRNA anticodon pairs with its complementary codon on the mRNA molecule, ensuring that the appropriate amino acid is inserted into the polypeptide. (genome.gov)
In a cell, antisense DNA serves as the template for producing messenger RNA (mRNA), which directs the synthesis of a protein. (genome.gov)
However, genes involved in energy and protein synthesis, which are prevalent in leaves, were expressed at a relatively low level. (scite.ai)

Analysis of these sequences revealed single base substitutions and single base indels are the most frequently observed form of sequence variation between genes within families in the dataset. (biomedcentral.com)

Thus, this study demonstrates that using proteomic methods on our transgenic model can uncover important in vivo APP-interacting proteins that will provide insights into the biology of APP. (jneurosci.org)
Thus, this study demonstrates that our transgenic model can uncover important in vivo APP-interacting proteins that will contribute to our understanding of APP processing in in vivo settings. (jneurosci.org)
To explore the means by which prions are transmitted with high efficiency among cervids, we examined prion infectivity in the apical skin layer covering the growing antler (antler velvet) by using CWD-susceptible transgenic mice and protein misfolding cyclic amplification. (cdc.gov)

failed verification] The translated NHLRC2 protein is 726 amino acids long in humans and has a molecular weight of 79,442.59 g/mol. (wikipedia.org)
A protein consists of one or more chains of amino acids (called polypeptides) whose sequence is encoded in a gene. (genome.gov)

NCBI GEO Profiles detailed several conditions under which NHLRC2 expression is increase in comparison to base expression levels. (wikipedia.org)
A total of 389 unigenes had significant homologies with known protein sequences at the NCBI database and the remaining 199 unigenes were designated unidentified genes. (korea.ac.kr)
Participants also had the opportunity to attend hands-on workshops on NCBI tools, presented by Peter Cooper from NCBI/NIH, and on protein evolution, presented by Hugh Nicholas and Troy Wymore, from the Pittsburgh Supercomputing Center. (biomedcentral.com)

Extraction and Solubilization of Proteins for Proteomic Studies - Richard M. Leimgruber 2. (nhbs.com)

The differential gene expression data analysis was performed using GeneChip 4.0 software. (cdc.gov)
This study provides a differential gene expression profile between TMJ fibrous and bony ankylosis. (medscimonit.com)

The sequence of these clones is approximately 93% complete to at least 4-fold coverage [ 7 ]. (biomedcentral.com)
Approximately 5,400 clones from each library were sequenced from both the 5' and 3' directions. (usda.gov)

Microbial pathogen recognition relies on several classes of germline-encoded pattern recognition receptors (PRRs), including C-type lectin receptors, nucleotide oligomerization domain-like receptors (NLR), RIG-I-like receptors (RLRs), and toll-like receptors (TLRs) ( 6 ). (frontiersin.org)
Most NLRs guard host proteins that are the direct targets of pathogen effectors. (ubc.ca)

Many, but not all, conditional cases involved an increase of NHLRC2 expression in various cancer types, including-but not limited to-the following: leukemia, lymphomas, breast & mammary gland cancer, colorectal cancer, Wilms' tumor, and lung cancer. (wikipedia.org)
Significantly higher expression was observed in tumor necrosis factor receptor in the TCA treatment group compared to the parent compound - TCE. (cdc.gov)
LPA receptors and ATX are upregulated in numerous cancer cell types and show expression patterns that correlate with tumor cell invasiveness. (betalifesci.com)
CEACAM5 overproduction enriched for an epithelial gene expression pattern and facilitated tumor outgrowth at metastatic sites. (kactusbio.com)

Two key mechanisms in this context are methylation and acetylation, both of which involve modifications to the DNA molecule or to the histone proteins around which DNA is wound. (prohealth.com)
Methylation generally serves to suppress gene transcription, meaning that genes with methyl tags are less likely to be expressed. (prohealth.com)
DNA methylation alters glucocorticoid receptor expression through modifications of chromatin structure. (deepdyve.com)
Most methyl tags are deposited onto the DNA nucleotide called cytosine (C) whenever it occurs next to the nucleotide guanine (G). This CpG methylation begins during gestation and can change across the lifespan. (spectrumnews.org)
Do autistic people have different patterns of methylation? (spectrumnews.org)
Other autism-related patterns include higher levels of methylation marks in genes involved in regulating the immune system, neurons and synaptic signaling. (spectrumnews.org)

The biological and pathological functions of ADAMTS proteases are determined broadly by their respective substrates and their interactions with proteins in the pericellular and extracellular matrix. (encyclopedia.pub)
Multiple processes have been implicated in age-related delayed healing, including altered gene expression, intrinsic cellular changes, and changes in extracellular milieu (including hormones). (biomedcentral.com)
Multiple processes have been implicated in cutaneous aging, including gene expression, intrinsic cellular change and an altered extracellular milieu. (biomedcentral.com)

Biotin labeled cRNA transcripts were synthesized, fragmented and hybridized to HuGeneFL GeneChip probe arrays representing more than 6800 human genes and expressed sequence tags. (cdc.gov)
The expression level of the great majority of transcripts was unaffected by cold treatment at 0°C for 72 h, whereas pollen tube growth and seed production were substantially reduced. (scite.ai)
1995). The expression patterns of any population of transcripts can be evaluated qualitatively and quantitatively by identifying. (scite.ai)
The sequence depth of MPSS allowed us to capture and quantify almost all the transcripts at a specific stage in the development of the grape berry. (biomedcentral.com)
The number and relative abundance of transcripts from stage II grape berries was defined using Massively Parallel Signature Sequencing (MPSS). (biomedcentral.com)

Epi-", a Greek term meaning "above" or "on top of," in the term epigenetics , refers to modifications that do not change the DNA sequence itself but can turn genes on or off, affecting how transcription factors read genes. (prohealth.com)
It does this by physically impeding the binding of transcription factors and other machinery necessary for gene expression. (prohealth.com)
Alternatively, it can also attract proteins that block gene transcription. (prohealth.com)
This results in a more open or "relaxed" chromatin structure, enabling greater access for the transcription machinery, thereby promoting gene expression. (prohealth.com)
In the vertebrate CNS, HB9/MNR2 transcription factors are specifically expressed in all somatic motoneurons and are necessary to distinguish motoneurons from interneurons, in part by repressing interneuron-specific gene expression. (jneurosci.org)
Together, vertebrate HB9/MNR2 transcription factors are expressed in all somatic motoneurons and excluded from interneurons. (jneurosci.org)
Transcription analysis showed that the expression levels of the HpDGAT2A , HpDGAT2D , and HpDGAT2E genes markedly increased under high light and nitrogen deficient conditions with distinct patterns, which led to significant TAG and EAST accumulation. (researchsquare.com)
Importantly, their approach has the potential to identify transcription factors that might be indirectly regulating the genes and, thus, would not be recognized by sequence-based analysis of the expressed gene promoters. (biomedcentral.com)
Research has long focused on transcription from our genes that uses RNA messengers to code for the formation of proteins, the building blocks of the body. (lu.se)

However, the four ADAMTS proteases show differences in the number and localization of predicted sites for posttranslational modifications, such as N-glycosylation ( http://www.cbs.dtu.dk/services/NetNGlyc/ ), O-fucosylation, and furin processing which could specify individual substrates, define protein-protein interactions, or govern autocatalytic properties that collectively distinguish these four ADAMTS proteases from each other. (encyclopedia.pub)
There are also readily reproducible methods for protein expression profiling, identifying protein-protein interactions, and protein chip technology, as well as a range of newly developed methodologies for determining the structure and function of a protein. (nhbs.com)
Interestingly, functionally redundant SNIPER1 and SNIPER2 can control the protein levels of diverse sNLRs and the interactions between SNIPER1 and sNLRs appear to be through the common nucleotide-binding (NB) domains of sNLRs. (ubc.ca)
[ 3 ] It is also important to understand that the same polypeptide sequence can produce many different patterns of interresidue or intraresidue interactions. (medscape.com)

Epigenetics, the study of changes in gene expression not caused by alterations in the DNA sequence, has revolutionized our understanding of genetics and its impact on health and longevity. (prohealth.com)
In a recent study we characterized estrogen-regulated changes in gene expression using a model of delayed wound healing in young mice that have been rendered hypogonadal by ovariectomization (hence removing any effects of 'intrinsic aging') [ 9 ]. (biomedcentral.com)

Eleven exons are transcribe in the protein-coding mRNA. (wikipedia.org)
An anticodon is a trinucleotide sequence located at one end of a transfer RNA (tRNA) molecule, which is complementary to a corresponding codon in a messenger RNA (mRNA) sequence. (genome.gov)
Here we performed mRNA profiling using Massively Parallel Signature Sequencing (MPSS) and combined it with available Expressed Sequence Tag (EST) data. (biomedcentral.com)

We discovered high levels of Pentatomicin expression in eggs, which is atypical of other AMPs and suggestive of its biological functioning in eggs. (bvsalud.org)

Our study provides essential structural information of unexplored proteins of the Hom family that can help in a better understanding of H. pylori pathogenesis. (nature.com)

The Autotaxin protein is important for generating the lipid signaling molecule lysophosphatidic acid (LPA), which is a potent mitogen, which facilitates cell proliferation and migration, neurite retraction, platelet aggregation, smooth muscle contraction, actin stress formation and cytokine and chemokine secretion. (betalifesci.com)
An amino acid is the fundamental molecule that serves as the building block for proteins. (genome.gov)

To assess the native APP interactome in brain, we generated mice expressing human APP-695 fused to a C-terminal affinity tag (APP-AT). (jneurosci.org)
Differentially regulated genes were validated at the level of gene and protein expression, with genes identified as estrogen-regulated in human confirmed as estrogen-dependent in young estrogen depleted mice in vivo . (biomedcentral.com)
These data show that GPI-anchored proteins of L. mexicana are not essential for growth of promastigotes, invasion of macrophages by promastigotes, or infection of mice. (gla.ac.uk)
Tg(CerPrP)1536 +/- mice expressing deer PrP have been described previously ( 4 ). (cdc.gov)
To generate Tg(CerPrP-E226) mice expressing the elk PrP coding sequence, codon 226 of the deer PrP gene ( PRNP ) (GenBank accession no. (cdc.gov)

D-aminoluciferin is as active as luciferin and provides a free -NH2 group for functionalization to attach peptide sequences corresponding to the cleavage site of a protease. (llnl.gov)
Other choices include calmodulin-binding peptide, Strep-tag (oxtapeptide binding to streptavidini), chitin-binding domain, and FLAG-tag (another octapeptide that binds to anti-FLAG mAbs). (genengnews.com)

We have characterized the global gene expression patterns of Arabidopsis pollen using Serial Analysis of Gene Expression (SAGE). (scite.ai)
Another technology, Serial Analysis of Gene Expression (SAGE), in part overcomes this limitation. (scite.ai)
SAGE allows simultaneous, comparative, and quantitative analysis of genespecific, 9-to 10-bp sequence tags (Velculescu et al. (scite.ai)
The output of the method is conceptually similar to a possibly more familiar method called Serial Analysis of Gene Expression (SAGE) [ 8 ]. (biomedcentral.com)

In a paper published in Molecular Ecology this month, Dr Alison Wright explains that the differences between chromosomes arise because of differences in the pattern of inheritance, which ultimately influences the number of chromosomes that are passed on to the next generation, called the effective population size. (ucl.ac.uk)
The majority of the autism brains displayed a 'convergent' subtype, displaying differences in gene expression and the patterns of chemical tags. (spectrumnews.org)
using a redundancy criterion to identify reproducible sequence differences between related genes within gene families. (biomedcentral.com)

However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. (betalifesci.com)
Only 10% of amyloidosis deposits consist of components such as glycosaminoglycans (GAGs), apolipoprotein-E (apoE), and serum amyloid P-component (SAP), while nearly 90% of the deposits consist of amyloid fibrils that are formed by the aggregation of misfolded proteins. (medscape.com)

Previous data has reported similarity between human brain and testis gene expression patterns. (karger.com)
Present results provide evidence to support the proposal that human testis and brain share the highest similarity of gene expression patterns. (karger.com)

The technology consists of a device that contains a FGM that is patterned to have a gradient in material properties (e.g. chemical affinity, surface chemistry, chirality, pore size, etc.) normal to the direction of flow of the mobile phase. (llnl.gov)
The addition of an affinity tag allowed us to avoid the use of antibodies targeted toward the intracellular C-terminal tail of APP, a region to which a number of reported interacting proteins bind ( King and Turner, 2004 ). (jneurosci.org)
Affinity tagging, a popular method for capturing or immobilizing proteins, has several incarnations, none more popular than polyhistidine tagging. (genengnews.com)
After expression, the protein is captured by means of immobilized metal affinity chromatography (IMAC). (genengnews.com)
Histidine tagging is by no means the only affinity tagging option. (genengnews.com)

Fluorophores for STORM and SMLM include synthetic dyes, fluorescent proteins (FPs), and even Quantum Dots (QDots). (microscopyu.com)
A reporter gene codes for a protein that can be tracked, such as a protein with a known enzymatic activity or one that is fluorescent. (jove.com)
Commonly used reporter genes are - GFP (green fluorescent protein gene), lacZ (β- galactosidase gene), RFP (red fluorescent protein gene), and Luc (luciferase gene). (jove.com)

Audic S, Claverie JM: The significance of digital gene expression profiles. (karger.com)

In the present study, we explored the structural aspect of HomA and HomB proteins using various computational, biophysical and small-angle X-ray scattering (SAXS) techniques. (nature.com)
The index is based on the integration of public transcript, protein, and mapping information, supplemented with computational prediction. (biomedcentral.com)
while John Weinstein, Chair of the Department of Bioinformatics and Computational Biology at the University of Texas M.D. Anderson Cancer Center, talked on "Personalizing Cancer Medicine in the Era of Next-Generation Sequencing: Omics and Informatics. (biomedcentral.com)

Researchers at LLNL have developed a new method to utilize highly selective molecular recognition events to attach proteins to any solid support through the C-terminus. (llnl.gov)
Researchers use reporter genes to determine when and where a gene of interest is expressed. (jove.com)
Researchers are finding new RNA all the time because sequencing is now so quick and cheap", says Sofi Elmroth, professor at the Division of Biochemistry and Structural Biology at Lund University. (lu.se)

Fractionation of Complex Proteomes by Microscale Solution Isoelectrofocusing Using ZOOM IEF Fractionators to Improve Protein Profiling - Xun Zuo, Ki-Beom Lee and David W. Speicher 13. (nhbs.com)

investigated pathogenesis and immunological response caused by HomB protein in clinical patients and 190 H. pylori strain isolated from patients with peptic ulcer disease (PUD) or gastritis were evaluated for the clinical importance of homB . (nature.com)
In the ODE, the protein concentration diminishes proportionally to the variable rate term \[ r_c (1 - \frac{c}{C_m}), \] where \(c\) is the cell density, \(r_c\) is the maximal cell growth rate, and \(C_m\) is the carrying capacity of the amplifier strain. (github.io)

YVAD" shows up three times alone in the human NHLRC2 protein. (wikipedia.org)
Mouse and human IL-17C share 83% aa sequence identity. (rndsystems.com)
The purity of Human ACE2 (18-615) Recombinant Protein (hFc-Tag) was determined by densitometry after SDS-PAGE of 1 μg of protein, followed by staining with Coomassie Blue. (cellsignal.com)
Binding response kinetics between Human ACE2 (18-615) Recombinant Protein (hFc-Tag) (immobilized) and SARS-CoV-2 Spike (trimeric) (16-1208) Recombinant Protein (8xHis-Tag) #65444 (in solution, at indicated concentrations). (cellsignal.com)
Recombinant Human CEACAM-5/CD66e (145-322) Protein is expressed from HEK293 with His tag at the C-terminus.It contains Pro145-Pro322. (kactusbio.com)
The predicted protein shares 31% identity with yeast and human homologues. (gla.ac.uk)
The "self"-protein albumin in mouse airway fluid was uniquely modified by GSH-MDI at position 414K, a preferred site of MDI reactivity on human albumin. (cdc.gov)
AN - check the tag INFANT HN - 2008 FX - Child Nutrition FX - Infant Nutrition Physiology FX - Milk FX - Milk, Human DH - Adolescent Nutrition DI - 052508 MN - SP6.021.067 MS - Nutrition of persons 10 through 19 years of age. (bvsalud.org)

Previous studies have identified four putative DGAT2-encoding genes in H. lacustris , and only HpDGAT2D allowed the recovery of TAG biosynthesis, but the engineering potential of HpDGAT2s in TAG biosynthesis remains ambiguous. (researchsquare.com)
Each sequence from this database was used to search public protein databases to assign putative function to as many database entries as possible. (usda.gov)

Similarly, expression of heat shock protein 70 increased 8.8 fold in TCA treatment group compared to TCE treatment which had 2.7 fold increase. (cdc.gov)
Similarly, formerly independent maps, based on cytogenetic banding patterns, meiotic crossovers and radiation hybrids, may be placed within the single consensus sequence. (biomedcentral.com)
This recombinant DNA was introduced into the worm using a microinjection, and the gene was expressed, similarly to β-tubulin. (jove.com)

Anatomy32

Organisms31

Diseases3

Chemicals and Drugs48

Analytical, Diagnostic and Therapeutic Techniques and Equipment46

Phenomena and Processes91

Disciplines and Occupations5

Technology, Industry, Agriculture4

Information Science12

Health Care2

Microarray10

ESTs5

Eukaryotic4

Transcriptional3

Recombinant Proteins1

Tissues12

Ribosomal protein2

Genome14

CDNA libraries1

Annotation1

Prion protein3

Functional10

Endoplasmic reticulum2

Libraries5

Cells26

Encodes4

Differentially expressed genes2

Bacterial2

Species3

Peptides2

Fluorescence3

Purification2

Residues4

Cytokines1

Organisms1

Nucleotide sequence1

Membrane9

Genomes2

Genetic11

Genomic3

Conformation1

Detect5

Synthesis4

Dataset1

Transgenic3

Amino acids2

NCBI3

Solubilization1

Differential gene expression2

Clones2

Pathogen2

Tumor4

Methylation6

Extracellular3

Transcripts5

Transcription9

Interactions4

Changes in gene2

MRNA3

Atypical1

Essential structural1

Molecule2

Mice5

Peptide2

Analysis4

Differences3

Aggregation2

Similarity2

Affinity5

Fluorescent3

Gene expression profiles1

Computational3

Researchers3

Profiling1

Strain2

Human8

Putative2

Similarly3