ComplexesSubunit of the vacuolarElectrochemical gradientPumpISOFORMPlasma membraneBiogenesisAdenosineYeastSaccharomycesLysosomalTranslocationVacuolesMembrane proteinsLumenGeneAcidificationMembranesEnzymeUptakeAcidicMammalianSecretory pathwayComplexMitogen activaHydrolysisInhibitorsReceptorMitochondrialPotassiumD12.776CytosolicActivityHomologousRoleInteractionsSubunitsBacteriaMechanismRegulationTransmembraneEukaryotesSequenceStrainAutophagyFunctionAbsenceMeSH
Complexes4
- Here, we report that overexpression of PEP3 which encodes a component of the HOPS and CORVET complexes involved in vacuolar biogenesis, shortened lag phase in Saccharomyces cerevisiae exposed to acetic acid stress. (oregonstate.edu)
- While individual V 1 and V o subcomplexes are locked in specific rotary states, the in vitro assembled complexes populated three rotary states, similar to what was previously observed for native V-ATPase. (upstate.edu)
- All such systems are multisubunit complexes with at least 3 dissimilar subunits embedded as a complex in the membrane (F 0 , a:b:c = 1:2:~12) and (usually) at least 5 dissimilar subunits attached to F 0 (F 1 , α:β:γ:δ:ε = 3:3:1:1:1 for F-type ATPases). (tcdb.org)
- The eukaryotic proteins are more complicated than the bacterial enzyme complexes. (tcdb.org)
Subunit of the vacuolar1
- To examine interactions between G proteins and the H + -ATPase, we performed immunocytochemical studies on perfusion-fixed sections of rat kidney using polyclonal anti-G protein antibodies and E11, a mouse monoclonal antibody to the 31-kDa subunit of the vacuolar H + -ATPase. (wustl.edu)
Electrochemical gradient2
- Yeast vacuoles contain an H + -ATPase that acidifies the vacuolar lumen and generates an electrochemical gradient of protons across their membranes. (tau.ac.il)
- abstract = "Yeast vacuoles contain an H+-ATPase that acidifies the vacuolar lumen and generates an electrochemical gradient of protons across their membranes. (tau.ac.il)
Pump10
- We show that XMEA is caused by hypomorphic alleles of the VMA21 gene, that VMA21 is the diverged human ortholog of the yeast Vma21p protein, and that like Vma21p, VMA21 is an essential assembly chaperone of the vacuolar ATPase (V-ATPase), the principal mammalian proton pump complex. (nih.gov)
- We have shown that this process is triggered by recruitment of ATG16L1 by the vacuolar-type H-translocating ATPase (V-ATPase) proton pump, suggesting a role for pH sensing in recruitment of Atg8-family proteins to single membranes. (babraham.ac.uk)
- The vacuolar ATPase (V-ATPase) is a highly conserved rotary motor proton pump that plays an essential role in cellular housekeeping functions. (upstate.edu)
- All eukaryotic F-type ATPases pump 3-4 H + out of mitochondria, or into thylakoids of chloroplasts, per ATP hydrolyzed. (tcdb.org)
- Bacterial F-type ATPases pump 3-4 H + and/or Na + (depending on the system) out of the cell per ATP hydrolyzed. (tcdb.org)
- V-type ATPases may pump 2-3 H + per ATP hydrolyzed, and these enzymes cannot catalyze pmf-driven ATP synthesis. (tcdb.org)
- The model also helped distinguish differences in malate concentration among the three cultivars and between the pre and post-harvest stages by highlighting the probable importance of proton pump activity and particularly of the free energy of ATP hydrolysis and vacuolar pH. (biomedcentral.com)
- The CV-system is also assumed to be involved in Ca 2+ -transport since it contains a PMCA-type Ca 2+ -ATPase (PAT1), calmodulin [ 3 ] and a vacuolar proton pump that establishes a proton gradient for Ca 2+ -transport [ 4 ]. (biomedcentral.com)
- Vacuolar-type ATPase: A proton pump to lysosomal trafficking. (doshisha.ac.jp)
- Some intercalated cells that stained prominently for the proton pump in the apical membrane did not, however, stain for any G protein α-subunit. (wustl.edu)
ISOFORM2
- V-ATPase a3 isoform mutations identified in osteopetrosis patients abolish its expression and disrupt osteoclast function. (doshisha.ac.jp)
- Isoform-specific gene disruptions reveal a role for the V-ATPase subunit a4 isoform in the invasiveness of 4T1-12B breast cancer cells. (doshisha.ac.jp)
Plasma membrane2
- In fungi, two recognized mechanisms contribute to pH homeostasis: the plasma membrane proton-pumping ATPase that exports excess protons and the vacuolar proton-pumping ATPase (V-ATPase) that mediates vacuolar proton uptake. (oregonstate.edu)
- For example, alkaline vacuole/lysosome are deficient in autophagy, Golgi pH regulates its ability to glycosylate proteins and failure to maintain endosomal pH perturbs with its ability to recycle receptors to the Plasma membrane or, the trans-Golgi. (upstate.edu)
Biogenesis2
- 1986. Protein sorting in yeast: mutants defective in vacuole biogenesis mislocalize vacuolar proteins into the late secretory pathway. . (ucsb.edu)
- This highly complex 'protein biogenesis' process is assisted by a diverse network of folding catalysts and protein-modifying enzymes and is scrutinized by molecular chaperones and other 'quality control' factors which ensure that only correctly folded and assembled proteins exit the ER and proceed to distal compartments of the secretory pathway. (stanford.edu)
Adenosine1
- ATPase H+ Transporting Accessory Protein 2 is associated with adenosine triphosphatases (ATPases). (creative-biolabs.com)
Yeast14
- 1990. Molecular analysis of the yeast VPS3 gene and the role of its product in vacuolar protein sorting and vacuolar segregation during the cell cycle. . (ucsb.edu)
- 1990. A putative GTP binding protein homologous to interferon-inducible Mx proteins performs an essential function in yeast protein sorting. . (ucsb.edu)
- 1989. Acidification of the lysosome-like vacuole and the vacuolar H+-ATPase are deficient in two yeast mutants that fail to sort vacuolar proteins. . (ucsb.edu)
- 1989. Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. . (ucsb.edu)
- 1989. Protein sorting in yeast: the role of the vacuolar proton-translocating ATPase. . (ucsb.edu)
- 1989. Protein targeting to the yeast vacuole. . (ucsb.edu)
- 1987. Protein sorting in yeast: the localization determinant of yeast vacuolar carboxypeptidase Y resides in the propeptide. . (ucsb.edu)
- Gene dosage-dependent secretion of yeast vacuolar carboxypeptidase Y. . J Cell Biol. (ucsb.edu)
- 1986. Overproduction-induced mislocalization of a yeast vacuolar protein allows isolation of its structural gene. . (ucsb.edu)
- A recent study from the Wilkens lab in collaboration with Seoul National University uncovered a novel mechanism of yeast V-ATPase regulation based on biochemical experiments and cryo electron microscopy (cryo-EM) structures of holo V-ATPase and Oxr1 bound V 1 subcomplex. (upstate.edu)
- used cryo-EM to obtain structures of yeast V-ATPase assembled from purified V 1 and V o subcomplexes. (upstate.edu)
- Further experiments will be required to uncover the physiological role of the interaction suof Oxr1 with the V-ATPase, and whether the observations obtained for the yeast system are conserved in higher organisms, including humans. (upstate.edu)
- When a 14-kDa polypeptide was suggested to be a subunit of a specialized V-ATPase in Manduca sexta, and a homologous short sequence was detected downstream of the UGA1 gene in yeast, we cloned this counterpart gene from yeast. (tau.ac.il)
- Epitope-tagged Vma7p was constructed and the proton uptake activity of isolated vacuoles from this yeast strain was markedly inhibited by a monoclonal antibody against the epitope-tag. (tau.ac.il)
Saccharomyces1
- 1986. PEP4 gene of Saccharomyces cerevisiae encodes proteinase A, a vacuolar enzyme required for processing of vacuolar precursors. . (ucsb.edu)
Lysosomal2
- Recent work published in The EMBO Journal and EMBO Reports reveals a novel role for the protein TECPR1 as a sensor for stressed membranes and regulator of lysosomal membrane repair. (babraham.ac.uk)
- ATPase, H+ transporting, lysosomal V0. (wikigenes.org)
Translocation3
- V-ATPase is made of two subcomplexes: a cytosolic V 1 that carries out ATP hydrolysis, and a membrane bound V o that is responsible for proton translocation. (upstate.edu)
- Electron cryoEM structures of mitochondrial, chloroplast, and bacterial ATP synthases have revealed the architecture of the FO region, helping to explain the mechanisms of proton translocation, dimerization of the enzyme in mitochondria, and cristae formation. (tcdb.org)
- The unique bifurcated oxidation of ubiquinol at center P (Qo) of the cytochrome bc1 complex is the reaction within the Q-cycle reaction scheme that is most critical for the link between electron transfer and vectorial proton translocation. (shengsci.com)
Vacuoles3
- By confocal microscopy, PEP3-overexpressing cells stained with the vacuolar membrane-specific dye, FM4-64 had more fragmented vacuoles than the wild-type control. (oregonstate.edu)
- V-type ATPases are found in vacuoles of eukaryotes and in bacteria. (tcdb.org)
- URA3 null mutants are not able to grow on a medium buffered at pH 7.5, they fail to accumulate quinacrine into their vacuoles and the other subunits of the catalytic sector are not assembled onto the vacuolar membrane in its absence. (tau.ac.il)
Membrane proteins2
- This list covers membrane proteins. (wikipedia.org)
- Membrane proteins must be threaded co-translocationally into the lipid bilayer to become membrane-integrated, often with complex topologies and typically form hetero- or homo- oligomers. (stanford.edu)
Lumen1
- The vast majority of proteins entering the secretory pathway are synthesized on ribosomes docked at ER translocons and are co-transationally translocated into the ER lumen. (stanford.edu)
Gene4
- In Dictyostelium discoideum , two PPK activities have been originally reported, one with sequence similarity to the bacterial PPK1 that was acquired through horizontal gene transfer [ 7 ], and a novel DdPPK2 activity, that seems to be a complex of three actin-related proteins: Arp1, Arp2, and an unreported Arpx [ 8 ]. (silverchair.com)
- The gene VMA7 encodes a protein Vma7p of about 14 kDa. (tau.ac.il)
- We also employed a LvsA minus strain which lacks the gene for the protein large volume sphereA ( lvsA ). (biomedcentral.com)
- The soxL gene from Sulfolobus acidocaldarius (DSM 639) encodes a Rieske iron-sulfur protein. (shengsci.com)
Acidification1
- Proton-translocating ATPases have fundamental roles in energy conservation, secondary active transport, acidification of intracellular compartments, and cellular pH homeostasis. (creative-biolabs.com)
Membranes1
- In non-canonical Atg8-family protein lipidation they become conjugated to single membranes. (babraham.ac.uk)
Enzyme2
- Thus, the enzyme renin acted upon its protein substrate, produced in the liver, to liberate the decapeptide angiotensin I which, upon circulating through the pulmonary circulation, finally produced the potent octapeptide angiotensin. (nshealth.ca)
- This download is the types and cells led from a human assembly target content soccer methylated alongside the set of two interaction localizing enzyme proteins in New Zealand. (evakoch.com)
Uptake1
- The decomposition of the organic matter, the imbalance of the N, S, and C cycles, the excess in the cation uptake on anions and the N fixation by the legumes influence the concentration of protons [H + ] in the soil solution. (frontiersin.org)
Acidic1
- Acidic means that the stores are equipped with a V-type H + -ATPase. (biomedcentral.com)
Mammalian1
- Guanosine 5′-triphosphate (GTP)-binding proteins (G proteins) are expressed in a heterogeneous manner in the mammalian kidney. (wustl.edu)
Secretory pathway1
- Our goal is to elucidate the functional networks that coordinate protein synthesis and quality control in the early secretory pathway. (stanford.edu)
Complex2
- The V-ATPase complex regulates non-canonical Atg8-family protein lipidation through ATG16L1 recruitment. (babraham.ac.uk)
- In particular, cells of the medullary collecting tubule demonstrate a complex pattern of G protein expression both between cell types and between the polarized surfaces of individual cells. (wustl.edu)
Mitogen activa1
- In the poster section, Joanna Shisler (University of Illinois at Urbana-Champaign [UIUC], Urbana) reported that the modified virus, Ankara, activates nuclear factor κB through the mitogen-activated protein kinase, extracellular signal–regulated kinase (MEK)/extracellular signal–regulated kinase (ERK) pathway, possibly facilitating the host immune response. (cdc.gov)
Hydrolysis2
- Unlike reversible disassembly, which requires ATP hydrolysis on V 1 to break protein-protein interactions, Oxr1p mediated disassembly of V-ATPase is ATP independent and therefore a novel mode of activity regulation. (upstate.edu)
- The rotor (which consists of the c, ε and γ subunits) is believed to rotate relative to the stator in response to either ATP hydrolysis by F 1 or proton transport through F 0 . (tcdb.org)
Inhibitors1
- Here, using pharmacological inhibitors, we investigate a role for V-ATPase during non-canonical autophagy. (babraham.ac.uk)
Receptor1
- ELAC1 has been in the use and may as define as an RNase Z. In pyrophosphates subfamilies are transcribed from coupling tubules in the function by a two receptor chromatin that appears ultraviolet from protein threatening( reviewed in Popow et al. (evakoch.com)
Mitochondrial3
- The F 1 portion of the bovine mitochondrial F-type ATPase has been solved to 2.8 Å resolution. (tcdb.org)
- Plant uncoupling mitochondrial protein activity in mitochondria isolated from tomatoes at different stages of ripening. (shengsci.com)
- The proton flow responsible for decreased pH values inside mitochondrial cristae and membrane invaginations of cyanobacteria has been c. (shengsci.com)
Potassium1
- According to the model, vacuolar composition, in particular potassium and organic acid concentrations, had an important effect on malate accumulation. (biomedcentral.com)
D12.7761
- For other protein-related codes, see List of MeSH codes (D12.776). (wikipedia.org)
Cytosolic2
- Although a vacuolar role in maintaining proper cytosolic pH has been proposed, the necessity for a functional vacuole is not completely understood [13] , and has probably a multifactorial nature that will be discussed further in other sections of this review. (microbialcell.com)
- Therefore, one can hypothesize that malate accumulation in fruit cells is mainly controlled at the level of vacuolar storage, and that metabolism responds appropriately to regulate the cytosolic concentration of malate since it plays a fundamental role in the regulation of cytosolic pH [ 14 ]. (biomedcentral.com)
Activity4
- Based on an indirect growth assay, the PEP3-overexpression strain exhibited higher V-ATPase activity. (oregonstate.edu)
- We hypothesize that PEP3 overexpression provides protection from acid stress by increasing vacuolar surface area and V-ATPase activity and, hence, proton-sequestering capacity. (oregonstate.edu)
- Not surprisingly, V-ATPase activity (or loss thereof) has been linked to several disease states including renal tubular acidosis, osteoporosis, neurodegeneration, and cancer. (upstate.edu)
- PPK2 is a dual activity 40.8 kDa protein that synthesizes polyP from GTP or ATP [ 5 ] and can use polyP as a donor to convert GDP to GTP [ 6 ]. (silverchair.com)
Homologous1
- The predicted protein is highly homologous to the above mentioned M. sexta protein. (tau.ac.il)
Role2
- These ATG proteins also perform an important parallel role in 'noncanonical' autophagy, a lysosome-associated signaling pathway with key functions in immunity, inflammation, cancer, and neurodegeneration. (babraham.ac.uk)
- He also showed that double-stranded (ds)RNA- and ZDNA binding proteins had a role in poxvirus pathogenesis. (cdc.gov)
Interactions2
- As the assembly was carried out in the absence of ATP, this suggests that formation of the holoenzyme from the individual subcomplexes is solely driven by protein-protein interactions at the interface. (upstate.edu)
- We prepared medullary membrane vesicles highly enriched for the H + -ATPase to examine possible functional interactions of G proteins with the H + -ATPase by the acridine orange method. (wustl.edu)
Subunits3
- Gα s subunits were consistently not associated with cells containing the H + -ATPase in this nephron segment, whereas Gα i-2 , Gα 1-3 , and Gα q/11 were. (wustl.edu)
- These vesicles were also highly enriched for G protein subunits. (wustl.edu)
- Gαs subunits were consistently not associated with cells containing the H+-ATPase in this nephron segment, whereas Gαi-2, Gα1-3, and Gαq/11 were. (wustl.edu)
Bacteria2
- F-type ATPases are found in eukaryotic mitochondria and chloroplasts as well as in bacteria. (tcdb.org)
- Na(+)-translocating NADH-quinone reductase of marine and halophilic bacteria. (shengsci.com)
Mechanism2
- By integrating these findings, we propose an updated and unifying mechanism for noncanonical autophagy, centered on ATG16L1 and V-ATPase. (babraham.ac.uk)
- Upon V0-V1 binding, V-ATPase recruits ATG16L1, via K490, during LC3-associated phagocytosis (LAP), STING- and drug-induced CASM, indicating a common mechanism. (babraham.ac.uk)
Regulation1
- However, the regulation of vacuolar malate storage throughout fruit development, and the origins of the phenotypic variability of the malate concentration within fruit species remain to be clarified. (biomedcentral.com)
Transmembrane3
- There are three classes of ATPases-F, P, and V. The vacuolar (V-type) ATPases have a transmembrane proton-conducting sector and an extramembrane catalytic sector. (creative-biolabs.com)
- The encoded protein has been found associated with the transmembrane sector of the V-type ATPases. (creative-biolabs.com)
- The crystal structure of the S. cerevisiae c-subunit ring with bound oligomycin revealed the inhibitor docked on the outer face of the proton-binding sites, deep in the transmembrane region ( Zhou and Faraldo-Gómez 2018 ). (tcdb.org)
Eukaryotes1
- Oxr1p belongs to the group of TLDc domain containing proteins that are highly conserved in higher eukaryotes. (upstate.edu)
Sequence2
- A glycine-rich sequence in the catalytic site of F-type ATPase. (shengsci.com)
- Affinity labeling and genetic studies on the glycine-rich sequence of the beta subunit of E. coli F-type ATPase are discussed. (shengsci.com)
Strain1
- While the vacuolar pH of the wild-type strain grown in the presence of 80 mM acetic acid was significantly higher than in the absence of added acid, no significant difference was observed in vacuolar pH of the overexpression strain grown either in the presence or absence of 80 mM acetic acid. (oregonstate.edu)
Autophagy4
- Autophagy is a fundamental catabolic process coordinated by a network of autophagy-related (ATG) proteins. (babraham.ac.uk)
- V-ATPase is a universal regulator of LC3-associated phagocytosis and non-canonical autophagy. (babraham.ac.uk)
- Together, these data identify V-ATPase as a universal regulator of CASM and indicate that SopF evolved in part to evade non-canonical autophagy. (babraham.ac.uk)
- Conjugation of the Atg8 (autophagy related 8) family of ubiquitin-like proteins to phospholipids of the phagophore is a hallmark of macroautophagy/autophagy. (babraham.ac.uk)
Function1
- The enzyme's proton pumping function is regulated by a unique process called "reversible disassembly", wherein V 1 dissociates from V o in a for example nutrient dependent manner. (upstate.edu)
Absence1
- Proton transport was significantly increased in the presence of guanosine 5′-O-(3-thiotriphosphate), and this held true in the absence of chloride. (wustl.edu)
MeSH1
- H(+)-K(+)-Exchanging ATPase" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)