• Protein Phosphatase 2A (PP2A) is a widely expressed family of protein phosphatases made of a core dimer, composed of a catalytic (C) subunit and a structural (A) subunit, in association with a third variable regulatory (B) subunit. (ac.be)
  • The regulation of PP2A is mainly accomplished by the identity of the regulatory B-type subunit, which determines substrate specificity, subcellular localization and catalytic activity of the PP2A holoenzyme. (ac.be)
  • The enzymes that dephosphorylate these three amino acids are classified into four groups on the basis of specific catalytic signatures/domain sequences and substrate preference. (ac.be)
  • The structure in complex with a substrate analogue suggests a catalytic mechanism that is distinct from those of known NADases, ADP-ribosyl cyclases and transferases. (nature.com)
  • RNA catalytic and binding interactions with proteins and small molecules are fundamental elements of cellular life processes as well as the basis for RNA therapeutics and molecular engineering. (nature.com)
  • Protein engineering in the α-amylase family: Catalytic mechanism substrate specificity and stability. (scialert.net)
  • Because the catalytic center of all Dus enzymes is conserved, it is unclear how the same protein fold can be reprogrammed to ensure that nucleotides exposed at spatially distinct faces of tRNA can be accommodated in the same active site. (rcsb.org)
  • We tracked the catalytic reaction of RGC and the free GC domain independently by UV-light induced release of GTP from the photolabile NPE-GTP substrate. (elifesciences.org)
  • These superhelical structures present an extensive solvent-accessible surface that is well suited to binding large substrates such as proteins and nucleic acids. (eu.org)
  • Moreover, as the word 'coding' refers to the relationship between nucleic acids and proteins, rather than the mere transcription of DNA into RNA, it is logical to call the strand with the mRNA sequence the coding strand, as in the first example. (genomicglossaries.com)
  • Our results suggest that the combined use of Roscovitine and Kenpaullone may be useful for identifying substrates and physiological roles of cyclin-dependent protein kinases, whereas the combined use of Kenpaullone and LiCl may be useful for identifying substrates and physiological roles of glycogen synthase kinase 3. (nih.gov)
  • Although intracellular signal transduction is often portrayed as a protein kinase 'domino effect', the counterbalancing function of phosphatases, and thus the control of phosphatase activity, is equally relevant to proper regulation of cellular function. (ac.be)
  • Kinase Substrates Library, Group II, biotinylat. (anaspec.com)
  • Glutamate excitation induced a rapid alteration in the AMP:ATP ratio that was associated with the activation of the AMP-activated protein kinase (AMPK). (jneurosci.org)
  • Notably, inhibition of the CaMKK (calmodulin-dependent protein kinase kinase) had little affect on GLUT translocation, whereas the inhibition or knockdown of AMPK (compound C, siRNA) activity prevented GLUT3 translocation to the cell surface after glutamate excitation. (jneurosci.org)
  • Gene therapy displacement of endogenous MCUb with a dominant-negative MCUb transgene (MCUb W246R/V251E ) in vivo rescued T2D cardiomyocytes from metabolic inflexibility and stimulated cardiac contractile function and adrenergic responsiveness by enhancing phospholamban phosphorylation via protein kinase A. We conclude that MCUb represents one newly discovered molecular effector at the interface of metabolism and cardiac function, and its repression improves the outcome of the chronically stressed diabetic heart. (diabetesjournals.org)
  • The AGC (cAMP-dependent, cGMP-dependent and protein kinase C) protein kinase family embraces a collection of protein kinases that display a high degree of sequence similarity within their respective kinase domains. (embl.de)
  • AGC kinase proteins are characterised by three conserved phosphorylation sites that critically regulate their function. (embl.de)
  • Scholars@Duke publication: Protein kinase C beta II specifically binds to and is activated by F-actin. (duke.edu)
  • The two most closely related isoenzymes of protein kinase C (PKC), PKC betaI and betaII, are distinct but highly homologous isoenzymes derived via alternative splicing of the same gene product. (duke.edu)
  • The binding of PKC betaII to actin was not inhibited by kinase inhibitors of PKC (sphingosine and staurosporine), suggesting that prior activation and/or substrate phosphorylation are not required for the interaction of PKC betaII with actin. (duke.edu)
  • In the past, most of the attention was focused primarily on protein kinases and on their regulation, mainly because phosphatases were then viewed as simple housekeeping enzymes. (ac.be)
  • But advances in the understanding of protein phosphatases make now clear that these enzymes are precisely regulated and are as important as kinases in the regulation of cellular processes involving protein phosphorylation. (ac.be)
  • This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. (cancerindex.org)
  • The enzymes display overlapping but distinct substrate specificity. (cancerindex.org)
  • Proteins that catalyze both functions are referred to as glycogen debranching enzymes (GDEs). (wikidoc.org)
  • The actions of some drugs as substrates or inhibitors of enzymes involved in xenobiotic metabolism are a common reason for hazardous drug interactions . (bionity.com)
  • In phase I a variety of enzymes act to introduce reactive and polar groups into their substrates. (bionity.com)
  • The exquisite substrate specificity of Dus enzymes is therefore controlled by a relatively simple mechanism involving major reorientation of the whole tRNA molecule. (rcsb.org)
  • Vogel, A. Expanding the substrate scope of enzymes: Combining mutations obtained by CASTing. (mpg.de)
  • Vogel, A. Expanding the range of substrate acceptance of enzymes: Combinatorial active-site saturation test. (mpg.de)
  • The described setup and experimental design enable real-time monitoring of substrate turnover in light-activated enzymes on a molecular scale, thus opening the pathway to a deeper understanding of enzyme activity and protein-protein interactions. (elifesciences.org)
  • The mode of action of enzymes and macromolecular complexes as protein machines will be investigated. (manchester.ac.uk)
  • Class I enzymes are totally O 2 -dependent and generate a stable tyrosyl radical on the protein through activation of O 2 by a di-iron centre. (lu.se)
  • Although viewed as a constitutive housekeeping enzyme in the past, PP2A is a highly regulated phosphatase and is emerging as an important regulator of multiple cellular processes involving protein phosphorylation. (ac.be)
  • 1 Reversible protein phosphorylation is an important regulatory mechanism that controls the activities of a myriad of proteins and is thus involved in virtually every major physiological process. (ac.be)
  • 4 While proteins can be phosphorylated on nine amino acids, serine, threonine and tyrosine phosphorylation are by far the most predominant in eukaryotic cells. (ac.be)
  • Reversible protein phosphorylation is an essential regulatory mechanism in eukaryotes. (eu.org)
  • 2017). Moreover, Golgi-associated Rab29 can recruit LRRK2 to the surface of the Golgi and activate it there for both auto- and Rab substrate phosphorylation. (stanford.edu)
  • Protein phosphorylation, which plays a key role in most cellular activities, is a reversible process mediated by protein kinases and phosphoprotein phosphatases. (embl.de)
  • Phosphorylation usually results in a functional change of the target protein by changing enzyme activity, cellular location, or association with other proteins. (embl.de)
  • Interestingly, MDR1 Pgp has been suggested to exhibit an unusually broad substrate specificity. (hu-berlin.de)
  • These systems therefore solve the specificity problem by possessing such broad substrate specificities that they metabolise almost any non-polar compound. (bionity.com)
  • Marchfelder, A. The tRNase Z family of proteins: physiological functions, substrate specificity and structural properties. (mpg.de)
  • Activity is regulated on two levels: overall activity and substrate specificity (as only one RNR catalyses the reduction of all four ribonucleotides under given physiological conditions). (lu.se)
  • Heterogeneity observed in the structural architecture of the RGS domain, as well as in engagement of switch III and the all-helical domain of the Galpha substrate, suggests that unique structural determinants specific to particular RGS protein/Galpha pairings exist and could be used to achieve selective inhibition by small molecules. (rcsb.org)
  • Messenger RNA (mRNA) molecules are encoded by genes and are themselves templates for the proteins that carry the main metabolic functions in a cell. (sciencedaily.com)
  • MicroRNAs are ~22 nucleotide long RNA molecules that provide substrate specificity to a protein complex known as the RNA-induced silencing complex. (sciencedaily.com)
  • Polar compounds cannot diffuse across these cell membranes and the uptake of useful molecules is mediated through transport proteins that specifically select substrates from the extracellular mixture. (bionity.com)
  • A synthetic, specially- designed oligonucleotide with the ability to recognize and bind a protein ligand molecule or molecules with high affinity and specificity. (genomicglossaries.com)
  • Within lysosomes, the enzyme helps break down complexes called glycoproteins and glycolipids, which consist of sugar molecules attached to certain proteins and fats. (medlineplus.gov)
  • Polypeptide substrate specificity of PsLSMT. (wikipedia.org)
  • The results are interpreted in terms of the available specificity theories and are compared with results obtained in the study of polypeptide substrates. (caltech.edu)
  • They degrade polypeptides and are distinguished by their substrate specificities. (eurogentec.com)
  • The formation of a complex then enables Lon to degrade free ribosomal proteins. (go.jp)
  • Papain has wide specificity and it will degrade most protein substrates more extensively than the pancreatic proteases. (worthington-biochem.com)
  • this is because they directly bind to their target proteins and thus control substrate specificity. (europa.eu)
  • A particular challenge is the synthesis of oligosaccharyltransferases (OSTs), which catalyze the attachment of glycans to specific amino acid residues in target proteins. (biorxiv.org)
  • Furthermore, we demonstrate the ability of cell-free synthesized OSTs to glycosylate multiple target proteins with varying N -glycosylation acceptor sequons. (biorxiv.org)
  • Structural analysis of hDJ-1 by molecular docking and simulation studies, however, indicates a possible role of glutamate (Glu18) in determining its substrate specificity . (bvsalud.org)
  • The principal objective of our broad, translational research is to determine structural attributes and to develop small-molecule compounds for new, emerging, cancer-related protein-protein interactions in the ubiquitin-proteasome system (UPS) and ubiquitin-like protein (UBL) conjugation pathways. (europa.eu)
  • The role of adenovirus structural proteins in the regulation of adenovirus infection. (microbiologyresearch.org)
  • By querying the literature and a recently available database of allosteric sites, we gathered 213 allosteric proteins with structural information that we further filtered into a non-redundant set of 91 proteins. (biomedcentral.com)
  • Furthermore, we implemented an approach that achieves 65% positive predictive value in identifying allosteric sites within the set of predicted cavities of a protein (stricter parameters set, 0.22 sensitivity), by combining the current analysis on dynamics with previous results on structural conservation of allosteric sites. (biomedcentral.com)
  • The structural specificity of α-chymotrypsin for polypeptides and denatured proteins has been examined. (caltech.edu)
  • After the spectroscopic characterization of the late rhodopsin photoproducts, we analyzed truncated variants and revealed the involvement of the cytosolic N-terminus in the structural rearrangements upon photo-activation of the protein. (elifesciences.org)
  • The ability of a protein to retain its structural conformation or its activity when subjected to physical or chemical manipulations. (bvsalud.org)
  • Using functional proteomics approaches, our lab focus on characterizing how protease substrates play critical roles in such vital processes, to advances our knowledge about the role of these proteases in human diseases. (ualberta.ca)
  • The Unique Cofactor Region of Zika Virus NS2B-NS3 Protease Facilitates Cleavage of Key Host Proteins. (ualberta.ca)
  • Substrate-specificity of the DNA-protein crosslink repair protease SPRTN. (uni-muenchen.de)
  • Additionally, for complex biological matrices, antibody selection can be challenging since protein targets secreted from cells into cell culture media or serum may be cleaved or modified, altering epitope recognition-hence the benefit of using protease cocktail inhibitors. (genengnews.com)
  • Here I propose to call the polyP-Lon complex the "stringent protease," and I discuss new insights of protein degradation control in bacteria. (go.jp)
  • The structure bound to the substrate analogue along with mutagenesis of predicted critical amino acid residues led us to suggest a reaction mechanism distinct from all known NADases. (nature.com)
  • Quantitative MS-based enzymology of caspases reveals distinct protein substrate specificities, hierarchies, and cellular roles. (ualberta.ca)
  • Different PTMs show distinct impacts on protein functions, and normal protein activities are consequences of all kinds of PTMs working together. (hindawi.com)
  • The long term goal of our research is to elucidate the molecular mechanisms by which proteins are targeted to specific and distinct compartments. (stanford.edu)
  • N-acylated peptide esters as substrates. (caltech.edu)
  • A series of N-acetylated peptide esters of varying length have been evaluated as substrates of α-chymotrypsin. (caltech.edu)
  • Here, the anionic PS was of particular concern, although previously reported not to be an MDR1 Pgp substrate. (hu-berlin.de)
  • In humans there exist five isoforms of the B56 type regulatory subunit and they bind to their interacting proteins through a conserved LxxIxE motif. (eu.org)
  • Unexpectedly, crystal structures of DusC complexes with tRNA(Phe) and tRNA(Trp) show that Dus subfamilies that selectively modify U16 or U20 in tRNA adopt identical folds but bind their respective tRNA substrates in an almost reverse orientation that differs by a 160° rotation. (rcsb.org)
  • Here we define the precise Rab29 binding region of the LRRK2 Armadillo domain between residues 360-450 and show that this domain, termed 'Site #1', can also bind additional LRRK2 substrates, Rab8A and Rab10. (stanford.edu)
  • They display considerable versatility in binding modes, even between members of the same class (e.g. some bind DNA, others protein), suggesting that Znf motifs are stable scaffolds that have evolved specialised functions. (embl-heidelberg.de)
  • Similar movements were observed for the class III enzyme but the full picture has still not been obtained as substrates were not observed to bind to the active site in this system. (lu.se)
  • We use nuclear magnetic resonance (NMR) and X-ray crystallography techniques to structurally characterize protein-protein interactions (PPI) and to develop small-molecule inhibitors for these PPIs. (europa.eu)
  • Adenovirus protein- protein interactions: hexon and protein VI. (microbiologyresearch.org)
  • These studies serve to define a novel functional domain in the carboxyl-terminal region of PKC beta, which is involved in directing isoenzyme-specific protein-protein interactions, and consequently, isoenzyme-specific functions in vivo. (duke.edu)
  • Although a number of important interactions by proteins such as selectins, galectins, and sialic acid-binding immunoglobulin-like lectins are thought to mainly rely on sulfated O-glycans, our insight into the sulfotransferases that modify these glycoproteins, and in particular GalNAc-type O-glycoproteins, is limited. (lu.se)
  • A single amino acid change (Glu18 to Asp18) improved the substrate specificity of mDJ-1 toward NacLys and NacArg. (bvsalud.org)
  • Protein kinases catalyse the transfer of the gamma phosphate from nucleotide triphosphates (often ATP) to one or more amino acid residues in a protein substrate side chain, resulting in a conformational change affecting protein function. (embl.de)
  • The hOAT3 cDNA consisted of 2179 base pairs that encoded a 543-amino-acid residue protein with 12 putative transmembrane domains. (aspetjournals.org)
  • Protein dephosphorylation by the PP2A phosphatase is mainly achieved through the interaction of its regulatory subunit with the associated proteins. (eu.org)
  • Specifically, by using lipid nanodiscs as cellular membrane mimics, we obtained yields of up to 440 µg/mL for the single-subunit OST enzyme, 'Protein glycosylation B' (PglB) from Campylobacter jejuni , as well as for three additional PglB homologs from Campylobacter coli, Campylobacter lari , and Desulfovibrio gigas . (biorxiv.org)
  • Synthesis and secretion of recombinant tick-borne encephalitis virus protein E in soluble and particulate form. (microbiologyresearch.org)
  • Regulator of G protein signaling (RGS) proteins accelerate GTP hydrolysis by Galpha subunits and thus facilitate termination of signaling initiated by G protein-coupled receptors (GPCRs). (rcsb.org)
  • The effects of an imidazoline compound (BL11282) on protein expression in rat pancreatic islets were investigated with a proteomic approach. (researchgate.net)
  • Carbohydrate binding sites in a pancreatic α-amylase-substrate complex derived from x-ray structure analysis at 2.1Ao resolution. (scialert.net)
  • The complex then cleaves the mRNA into smaller pieces, thereby preventing translation of the protein it encodes, and thus inhibiting or "silencing" gene expression. (sciencedaily.com)
  • There are many AGO1-like proteins in animals and other eukaryotes as well, indicating that the RNA-induced silencing complex is of ancient evolutionary origin, and that microRNA-mediated regulation of gene expression is shared among many eukaryotes. (sciencedaily.com)
  • Gene polymorphisms influence protein concentration and substrate specificity, as do some occupational and lifestyle factors. (cdc.gov)
  • What does this gene/protein do? (cancerindex.org)
  • What pathways are this gene/protein implicaed in? (cancerindex.org)
  • In E. coli , Glucose transfer is performed by 4-alpha-glucanotransferase, a 78.5 kDa protein coded for by the gene malQ. (wikidoc.org)
  • methylation at certain residues of histones can regulate gene expression [ 4 ], and glycosylation is responsible for targeting substrates and changing protein half-life [ 2 ]. (hindawi.com)
  • Either at genomic or at proteomic level, mutations have significant impact on normal gene or protein function, and human diseases could be associated with mutations like nonsynonymous single-nucleotide variations (nsSNVs) on amino acids. (hindawi.com)
  • Yet how gene mutations affect protein activities through posttranslational modification sites have not been widely studied. (hindawi.com)
  • The hOAT3 protein was shown to be localized in the basolateral membrane of renal proximal tubules and the hOAT3 gene was determined to be located on the human chromosome 11q12-q13.3 by fluorescent in situ hybridization analysis. (aspetjournals.org)
  • We stably engineered O-glycan sulfation capacities in HEK293 cells by site-directed knockin of sulfotransferase genes in combination with knockout of genes to eliminate endogenous O-glycan branching (core2 synthase gene GCNT1) and/or sialylation capacities in order to provide simplified substrates (core1 Galβ1-3GalNAcα1-O-Ser/Thr) for the introduced sulfotransferases. (lu.se)
  • In the past years, several proteins were suggested to transport lipids between the leaflets of a membrane, and to potentially influence transverse lipid asymmetry and related cell properties. (hu-berlin.de)
  • The mature flavivirus particle comprises a nucleocapsid core surrounded by a lipid bilayer containing the membrane (M) (derived from the precursor prM) and envelope (E) proteins. (microbiologyresearch.org)
  • Differential stability of the bovine prion protein upon urea unfolding. (ualberta.ca)
  • Protein glycosylation, or the attachment of sugar moieties (glycans) to proteins, is important for protein stability, activity, and immunogenicity. (biorxiv.org)
  • PTMs are involved in many protein activities and cellular processes, such as protein folding, stability, conformation, and some significant regulatory mechanisms [ 1 ]. (hindawi.com)
  • Glycation is a non-enzymatic reaction wherein sugars or dicarbonyls such as methylglyoxal (MGO) and glyoxal (GO) react with proteins , leading to protein inactivation. (bvsalud.org)
  • The solution that has evolved to this problem is an elegant combination of physical barriers and low-specificity enzymatic systems. (bionity.com)
  • Such reprogramming of the enzymatic specificity appears to be a unique evolutionary solution for altering tRNA recognition by the same protein fold. (rcsb.org)
  • The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. (lu.se)
  • On the other hand, the interaction of PKC betaII with actin resulted in marked enhancement of autophosphorylation of PKC betaII and in an alteration in substrate specificity. (duke.edu)
  • We have previously examined the specificities of 28 commercially available compounds, reported to be relatively selective inhibitors of particular serine/threonine-specific protein kinases [Davies, Reddy, Caivano and Cohen (2000) Biochem. (nih.gov)
  • Moreover, the enzyme activity bands formed using 1-NA proves the specificity of the substrate for hemolysate cholinesterase as in the presence of specific acetylcholinesterase inhibitors the band formation disappears. (who.int)
  • This report represents the first description of combined label-free quantitative proteomics and PRM analysis of targeted proteins for discovery of different proteins before and after IVR treatment in the same patient. (frontiersin.org)
  • The 1.6 Å X-ray structure of the homodimeric A. fumigatus protein reveals unique properties including N-linked glycosylation and a Ca 2+ -binding site whose occupancy regulates activity. (nature.com)
  • Mutagenesis of the NS2B-NS3-mediated cleavage site in the flavivirus capsid protein demonstrates a requirement for coordinated processing. (microbiologyresearch.org)
  • [9] A second protein, referred to as debranching enzyme, performs α-1,6-glucose cleavage. (wikidoc.org)
  • Class III RNRs are strictly anaerobic and generate a stable glycyl radical on the protein by cleavage of S- adenosylmethionine, which can be regarded as a homologue of adenosylcobalamin. (lu.se)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • However, for most allosteric proteins identified to date the mechanistic details of allosteric modulation are not yet well understood. (biomedcentral.com)
  • In this article, we describe a simple computational approach, based on the effect allosteric ligands exert on protein flexibility upon binding, to predict the existence and position of allosteric sites on a given protein structure. (biomedcentral.com)
  • We performed normal-mode analysis and observed significant changes in protein flexibility upon allosteric-ligand binding in 70% of the cases. (biomedcentral.com)
  • These results agree with the current view that allosteric mechanisms are in many cases governed by changes in protein dynamics caused by ligand binding. (biomedcentral.com)
  • We introduce a simple computational approach to predict the presence and position of allosteric sites in a protein based on the analysis of changes in protein normal modes upon the binding of a coarse-grained ligand at predicted cavities. (biomedcentral.com)
  • Its performance has been demonstrated using a newly curated non-redundant set of 91 proteins with reported allosteric properties. (biomedcentral.com)
  • More recently we have probed the allosteric regulation of class II and class III RNRs by solving the structures of complexes with several combinations of allosteric effector and substrate [2,5]. (lu.se)
  • Epithelial cells adjacent to the area of the defect flatten, lose their hemidesmosome attachments, and migrate on transient focal contact zones that are formed between cytoplasmic actin filaments and extracellular matrix proteins. (medscape.com)
  • New insights into the specificity of thioredoxin function. (nih.gov)
  • Proteins can be regarded as the functional building blocks of life, carrying out and coordinating almost all biological processes. (biomedcentral.com)
  • Protein posttranslational modifications (PTMs) play key roles in a variety of protein activities and cellular processes. (hindawi.com)
  • Research reported in The Plant Cell shows that microRNAs control the accumulation of transcription factor proteins that regulate the expression of genes in the auxin response pathway. (sciencedaily.com)
  • The importance of a binding interaction between the carboxyl function of the substrate and the enzyme is suggested by the results obtained. (caltech.edu)
  • The RING domain is a protein interaction domain that has been implicated in a range of diverse biological processes. (embl-heidelberg.de)
  • Temperature sensitivity of processing of viral proteins. (microbiologyresearch.org)
  • With any type of laboratory test, the clinical accuracy or reliability depends on performance characteristics such as sensitivity and specificity, as well as the pretest probability that a person has SARS-CoV-2 infection and the prevalence of COVID-19 in the local community. (medscape.com)
  • Our results prove that 1-NA is an alternative substrate of hemolysate cholinesterase which specifically detects the enzyme activity on gel rapidly. (who.int)
  • Of these, indirubin-3'-monoxime, SP 600125, KT 5823 and ML-9 were found to inhibit a number of protein kinases and conclusions drawn from their use in cell-based assays are likely to be erroneous. (nih.gov)
  • Dual specificity protein kinases (e.g. (embl.de)
  • High-throughput sequencing-RNA affinity profiling (HiTS-RAP) 22 is conceptually similar to RNA-MaP, but uses the E. coli replication terminator protein Tus to stall the RNA polymerase after transcribing the variable section of the DNA library. (nature.com)
  • Bioinformatics analysis indicated the up-regulated proteins were significantly enriched in "GnRH secretion" and "Circadian rhythm" signaling pathway. (frontiersin.org)
  • We also study the NPC1 protein that is essential for cholesterol transport in humans and can lead to Niemann Pick C disease when mutated. (stanford.edu)
  • Cathepsin D is the lysosomal aspartic proteinase, active in intracellular protein breakdown. (eurogentec.com)
  • Thus, a comprehensive understanding of the activation and inactivation of these novel proteins is of utmost interest. (elifesciences.org)
  • 2 Protein phosphatase 2A (PP2A) is a very abundant - it accounts for as much as 1% of total cellular proteins - ubiquitous and remarkably conserved enzyme. (ac.be)
  • HEAT repeat domain, found in protein phosphatase 2a and initiation factor eIF4G. (eu.org)
  • Protein phosphatase 2A (PP2A) belongs to the superfamily of phosphoprotein phosphatases (PPPs) and catalyzes protein dephosphorylation by hydrolyzing Ser/Thr-linked phosphate ester bonds ( Heroes,2013 ). (eu.org)
  • Finally, some reactive esters of N-acetylated amino acids have been evaluated as substrates of α-chymotrypsin. (caltech.edu)
  • C-reactive protein is considered one of the best measures of the acute phase response to an infectious disease or other cause of tissue damage and inflammation. (cdc.gov)
  • This method quantifies C-reactive protein (CRP) by latex-enhanced nephelometry. (cdc.gov)
  • dNTP products regulate the enzyme's specificity for the NTP or NDP substrates. (lu.se)
  • Microbial rhodopsins (Rhs) are transmembrane proteins that utilize light-induced isomerization of their retinal cofactor to function as light-sensitive ion channels, pumps and sensors. (elifesciences.org)
  • A large and still-growing number of PP2A substrates have been identified, which makes PP2A an important player in the regulation of a plethora of cellular processes. (ac.be)
  • PP2A-mediated protein dephosphorylation is involved in a broad range of cellular processes including cell-cycle progression, cytoskeletal dynamics, and growth factor signalling. (eu.org)
  • We found that DppA2 shows the highest flexibility on substrate recognition and that DppA2 and DppA4 have a higher tendency to utilize tripeptides. (nih.gov)
  • Vogel, A. Exosite Modules Guide Substrate Recognition in the ZiPD/ElaC Protein Family. (mpg.de)
  • A second report focuses on the function of the protein ARGONAUTE1 (AGO1), a major component of the RNA-induced silencing complex in Arabidopsis. (sciencedaily.com)
  • The motif is conserved in essential proteins throughout the eukaryotic domain of life and also in human viruses, suggesting that the motifs are required for basic cellular function. (eu.org)
  • Control of protein function through oxidation and reduction of persulfidated states. (nih.gov)