Proteins protein array analysis. Medical search. Frequent questions

AbstractReverse-phase protein array (RPPA) technology uses panels of high-specificity antibodies to measure proteins and protein post-translational modifications in cells and tissues. (curie.fr)
Our findings indicate that proteomic analyses of cancer cell lines using different RPPA platforms can identify concordant profiles of response to pharmacological inhibition, including when using different antibodies to measure the same target antigens. (curie.fr)
A) Wild-type H5N1 2.3.4 (KS) and B) H5N8 (QR) H5 proteins were applied to the glycan array precomplexed with StrepMAB-classic (IBA GmbH, Göttingen, Germany) and fluorescent secondary antibodies. (cdc.gov)
Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. (abcam.com)
We sought to determine if there was antibody deposition in SAH livers and whether antibodies extracted from SAH livers were cross-reactive against both bacterial antigens and human proteins. (elifesciences.org)
Employing human proteome arrays, we profiled the antibodies extracted from explanted SAH, alcoholic cirrhosis (AC), nonalcoholic steatohepatitis (NASH), primary biliary cholangitis (PBC), autoimmune hepatitis (AIH), hepatitis B virus (HBV), hepatitis C virus (HCV) and HD livers and found that antibodies of IgG and IgA isotypes were highly accumulated in SAH and recognized a unique set of human proteins as autoantigens. (elifesciences.org)
The use of an E. coli K12 proteome array revealed the presence of unique anti- E. coli antibodies in SAH, AC or PBC livers. (elifesciences.org)
This important study tested the hypothesis that liver-derived but not serum-derived antibodies that are cross-reactive to E.coli and to host proteins can play a role in the hepatic damage found in severe alcoholic hepatitis (SAH). (elifesciences.org)

As an alternative to planar arrays, a system based on color-coded beads for the creation of antibody arrays in suspension has become available to offer a microtiter plate-based option for screening larger number of samples with variable sets of capture reagents. (nih.gov)
BioChain's Universal Protein Lysates have been specifically designed for use as a control for antibody arrays and Western Blot Analysis. (biochain.com)

Our total protein tissue lysates are useful tools in expression analysis by identifying tumor- or disease-specific proteins and genes. (biochain.com)
Matched Pair Total Protein is a useful tool for accelerating gene expression analysis by identifying tumor-specific and tumor metastatic proteins and genes. (biochain.com)
Below is a non-exhaustive list of in-house infrastructures that are categorized into three overarching themes: bio-imaging, proteins, genes & cells and other resources. (lu.se)
In addition to infrastructures for bioimaging, protein and genes & cells, we also provide other resources e.g., databases, networks and specialized labs. (lu.se)
Immune system is a complex machinery, which is based on the highly coordinated expression of a wide array of genes and proteins. (lu.se)
A detailed analysis can be read also here on the evolution of the immunome genes. (lu.se)
Here you can search for group of genes e.g. by protein domains or ontology terms. (lu.se)
Here you can search for genes and proteins by GenBank gi numbers, accession numbers or UniProt accession numbers. (lu.se)
We analysed the genomic sequences to identify serotypes and penicillin-binding protein (PBP) genes 1A, 2B and 2X, and other resistance determinants, to predict minimum inhibitory concentrations (MICs) against penicillin, erythromycin, clindamycin, co-trimoxazole and tetracycline. (cdc.gov)
Chromosome 9 likely contains 800 to 900 genes that provide instructions for making proteins. (medlineplus.gov)

An antibody-protein array of putative immunogenic proteins was developed from a combined bioinformatic, experimental, and literature-based prioritization of homologous parasite proteins. (frontiersin.org)
Integrated bioinformatic analyses of selected mRNAs and lncRNAs were performed. (cancerindex.org)

Applying directly labeled samples onto arrays instead of using sandwich assays offers an approach to facilitate a systematic, high-throughput, and flexible exploration of protein profiles in body fluids such as serum or plasma. (nih.gov)
The interaction of sGAG with α2-macroglobulin receptor-associated protein (LRPAP1), exportin-1 (XPO1), and serine protease HTRA1 (HTRA1) was confirmed in reverse assays. (degruyter.com)
Using a solid methodology that includes state-of-the-art microscopy, proteome arrays, and gene ontology assays, it provides strong evidence that liver-derived IgG and IgA with cytotoxic properties and reactivity to both gut-derived E.coli and autoantigens accumulated in hepatocytes of SAH patients but not of healthy controls. (elifesciences.org)

We studied the influence of histological subtype on protein expression with reverse phase protein array (RPPA) and assessed proteomic changes associated with ARID1A mutation/BAF250a expression in EC and CCC. (biomedcentral.com)
Proteomic assessment of CCC and EC demonstrates remarkable differences in protein expression that are dependent on histotype, thereby further characterizing these cancers. (biomedcentral.com)
We aimed at identifying novel anti-invasion mechanisms of YC-1 in NPC by a functional proteomic platform, the reverse phase protein array (RPPA). (elsevierpure.com)

Membrane proteins are critical mediators for tumor progression and present enormous therapeutic potentials. (mit.edu)
Although gene profiling can identify their cancer-specific signatures, systematic correlations between protein functions and tumor-related mechanisms are still unclear. (mit.edu)

In a second experimental approach we have used a variant of the WISE (Whole Interactome Scanning Experiment) strategy to probe a large number of naturally occurring and chemically-synthesized peptides arrayed at high density on a glass surface. (torvergata.it)
the creation of a large number of compounds (usually proteins or peptides) through technologies such as phage display . (genomicglossaries.com)

We have shown that this assay system allows detecting proteins down into lower pico-molar and higher pg/ml levels with dynamic ranges over three orders of magnitude. (nih.gov)
Presently, this workflow enables the profiling of 384 samples for up to 384 proteins per assay. (nih.gov)
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. (abcam.com)
The Committee also reviewed preliminary data on a diagnostic assay based on protein-array technology, and agreed that additional studies will be needed to evaluate its robustness, stability, sensitivity and specificity. (who.int)

Consecutive pathway and cluster analysis led to the identification of biological processes, namely processes involving binding and processing of nucleic acids, LRP1-dependent endocytosis, and exosome formation. (degruyter.com)
Nucleic acids, proteins and morphology of the sample are now stable up to 7 days at room temperature, or for longer periods at 2-8°C, or even at -20°C and -80°C. Stabilized samples can be embedded in paraffin for histological studies. (qiagen.com)
Fixatives that contain formaldehyde crosslink biomolecules and modify nucleic acids and proteins. (qiagen.com)
PAXgene Tissue reagents in prefilled containers and PAXgene Tissue Kits provide a complete preanalytical solution for collection, fixation, and stabilization of tissue, and purification of high-quality nucleic acids for molecular research analysis. (qiagen.com)
Nucleic acids and proteins can be isolated from the stabilized samples before or after embedding in paraffin. (qiagen.com)

CrMP-Sol also presents QTY-enabled water-soluble designs to facilitate native membrane protein studies despite natural hydrophobicity. (mit.edu)
The database can accelerate therapeutic developments and biotechnological applications of cancer-related membrane proteins. (mit.edu)
A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available here . (abcam.com)
The protein is membrane associated and thus probably interacts with other membrane proteins. (huji.ac.il)
However, the vast majority of known NS3 host partners are soluble proteins rather than membrane proteins, most likely due to lack of appropriate platforms for their discovery. (huji.ac.il)
Utilization of an integrated microfluidics platform enables analysis of membrane proteins in their native form. (huji.ac.il)
We screened over 2800 membrane proteins for interaction with NS3 and 90 previously unknown interactions were identified. (huji.ac.il)
Takata K, Matsuzaki T, Tajika Y. Aquaporins: water channel proteins of the cell membrane. (benthamscience.com)

Proteins that activated immunity are potential antigens for immunization and the multi-omics phylum-spanning prioritization database that was created is a valuable resource for identifying target proteins in a wide array of different parasitic nematodes. (frontiersin.org)
Alongside the increasing availability of affinity reagents, antibody microarrays have become a powerful tool to screen for target proteins in complex samples. (nih.gov)

A cocktail of five recombinant proteins optimized for conserved GI nematode targets was used immunize pigs and test for active antibody responses in both the serum and intestinal ileal fluid of immunized pigs. (frontiersin.org)
Glycan array analysis of recombinant H5 proteins of influenza A viruses. (cdc.gov)

To this scope, genome sequences of both strains were obtained and subjected to in silico analyses. (frontiersin.org)

The line also includes the Fast Quanto micro ELISA arrays with specific cytokine antibody panels, and the Serum Biomarker Chip, a rante of multiplexed immunoassays for measuring key cellular growth proteins, together wtih accessories and comsumables. (genengnews.com)
METHODS: The serum IL-6 concentration in 110 OSCC patients and 109 healthy controls were determined by chemiluminescence analysis. (bvsalud.org)

Employing multiple RPPA-based approaches operated across distinct laboratories, we characterised a range of human breast cancer cells and their protein-level responses to two clinically relevant cancer drugs. (curie.fr)
We integrated multi-platform RPPA data and used unsupervised learning to identify protein expression and phosphorylation signatures that were not dependent on RPPA platform and analysis workflow. (curie.fr)
These results highlight the robustness and the reproducibility of RPPA technology and its capacity to identify protein markers of disease or response to therapy. (curie.fr)

Machine learning was used to extract key functional descriptions for protein visualization in the 3D-space, where spatial distributions provide function-based predictive connections between proteins and cancer types. (mit.edu)
LaBaer, J & Ramachandran, N 2005, ' Protein microarrays as tools for functional proteomics ', Current Opinion in Chemical Biology , vol. 9, no. 1, pp. 14-19. (elsevierpure.com)
Also list of proteins for functional categories, e.g. (lu.se)

The protein encoded by this gene is a mitogen-inducible monokine and is one of the major HIV-suppressive factors produced by CD8+ T-cells. (cancerindex.org)
What does this gene/protein do? (cancerindex.org)
What pathways are this gene/protein implicaed in? (cancerindex.org)
For Illumina CEO Jay Flatley and Affymetrix CEO Frank Witney, the future commercial potential of the maturing technology lies in applied markets, including cytogenetics, agricultural biotechnology, and direct-to-consumer services, rather than in the large array-driven genome-wide association or gene expression studies of years ago. (genomeweb.com)
The protein produced from the BCR-ABL1 gene signals cells to continue dividing abnormally and prevents them from self-destructing, which leads to overproduction of the abnormal cells. (medlineplus.gov)

Families of conserved protein domains, specialized in mediating interactions with short linear peptide motifs, are responsible for the formation of a variety of dynamic complexes in the cell. (torvergata.it)
To evaluate the quality of the resulting interaction dataset, we retested some of the interactions on a smaller scale and performed a series of pull down experiments on native proteins. (torvergata.it)
The newly discovered protein interactions may provide insights into HCV infection mechanisms and potentially provide new therapeutic targets against HCV. (huji.ac.il)
RNA catalytic and binding interactions with proteins and small molecules are fundamental elements of cellular life processes as well as the basis for RNA therapeutics and molecular engineering. (nature.com)
Despite these challenges there has been a marked increase in the use of protein microarrays to map interactions of proteins with various other molecules, and to identify potential disease biomarkers, especially in the area of cancer biology. (elsevierpure.com)
co-immunoprecipitation Used to determine protein- protein interactions . (genomicglossaries.com)
Our facilities provide the opportunity to study protein structure, molecular probes and drug design, system biology and molecular interactions in cells and tissues. (lu.se)

The cytokine array is analyzed using the same methods as a chemiluminescent western blot. (abcam.com)
If you are interested in this cytokine array, array ab133998 may also be of interest. (abcam.com)

Screening the array with sera and ileal fluid samples from immunized pigs suggested cross-reactivity among homologous proteins and a general activation of immunity. (frontiersin.org)
Immumome contains information about immunity related proteins, their domain structure and the related ontology terms. (lu.se)

abstract = "The hepatitis C virus (HCV) non-structural protein 3 (NS3) is essential for HCV maturation. (huji.ac.il)

These included PI3K/AKT pathway proteins, those regulating the cell cycle, apoptosis, transcription, and other signaling pathways including steroid hormone signaling. (biomedcentral.com)
Assembly of collagen fibrils and other multimeric structures_Homosapiens_R-HSA-2022090 in pathway analysis and progesterone_CTD_00006624 in DSigDB analysis were the most significant and SLC2A1, hsa-miR-188-3p, hsa-miR-378a-3p and hsa-miR-150-5p were selected as survival markers. (cancerindex.org)

High-throughput sequencing-RNA affinity profiling (HiTS-RAP) 22 is conceptually similar to RNA-MaP, but uses the E. coli replication terminator protein Tus to stall the RNA polymerase after transcribing the variable section of the DNA library. (nature.com)
Dr. Cochran's translational interests span protein-based drug discovery and development for applications in oncology and regenerative medicine, and the development of new technologies for high-throughput protein analysis and engineering. (stanford.edu)

RESENSA (Receptor S-layer Electrical Nano Sensing Array)- the device detects the same molecules that natural cell receptors do. (mit.edu)
A synthetic, specially- designed oligonucleotide with the ability to recognize and bind a protein ligand molecule or molecules with high affinity and specificity. (genomicglossaries.com)
The basic format of the yeast-two hybrid system involves the creation of two hybrid molecules, one in which the " bait ' protein is fused with a transcription factor, and one in which the " prey " protein is fused with a related transcription factor . (genomicglossaries.com)

SAM (Significance Analysis of Microarrays) was performed to determine differential protein expression by histological subtype and ARID1A mutation status. (biomedcentral.com)
Protein microarrays present an innovative and versatile approach to study protein abundance and function at an unprecedented scale. (elsevierpure.com)
Given the chemical and structural complexity of the proteome, the development of protein microarrays has been challenging. (elsevierpure.com)
In this review, we discuss some of the promising advances made in the development and use of protein microarrays. (elsevierpure.com)
Recently, major breakthroughs in miniaturization of molecular analysis have produced DNA and protein microarrays. (nycu.edu.tw)
Stu Borman, Chemical and Engineering News, Dec. 16, 2002 lists as highlights of 2002 http://pubs.acs.org/cen/coverstory/8050/8050chemhighlights5.html with references to the literature on polysaccharide and glycoconjugate microarrays, monosaccharide chips, natural and synthetic oligosaccharide arrays, and synthetic oligosaccharides in microtiter plate format. (genomicglossaries.com)

We offer Universal Protein Lysates from humans and mice, as well as customized samples from a variety of species to fit your needs. (biochain.com)

Here, we used a genomics/proteomics approach (including immunoblot experiments from pigs infected with T. suis ) to prioritize putative immunogenic excretory/secretory (E/S) proteins conserved across and specific to several gastrointestinal (GI) parasitic nematode species. (frontiersin.org)
We are proud to work together with the leading biotech company in Sweden for biomarker panel analyses in epidemiology, OLINK Proteomics AB, Uppsala, and its representatives! (lu.se)

Here we used an affinity-purification mass spectrometry-based (AP-MS) approach to identify novel and particularly intracellular sGAG-interacting proteins in human bone marrow stromal cells (hBMSC). (degruyter.com)
We offer sample collection + preparation, extraction & purification, and sample analysis services. (biochain.com)
PAXgene Tissue Kits and supplementary protocols provide efficient subsequent purification of RNA, miRNA, DNA, and/or proteins from the same sample. (qiagen.com)
In addition, supplementary protocols for protein purification and other applications are available in the 'Resources' section of this page or at www.preanalytix.com . (qiagen.com)

Significant miRNA families were identified from mRNA-miRNA and lncRNA-miRNA interaction network analyses followed by stage specific mRNA-miRNA-lncRNA association network generation. (cancerindex.org)

With the help of various forms of mass spectrometry, synchrotron radiation, protein production & labelling, and bioinformatics, our facilities provide the opportunity to study protein structure and dynamics, molecular probes and drug design. (lu.se)

By identifying many sGAG-specific interacting proteins, our data provide a resource for upcoming studies aimed at molecular mechanisms and understanding of sGAG cellular effects. (degruyter.com)
at the same time developing protein and peptide-based tools that will allow us to manipulate cellular processes on a molecular level. (stanford.edu)

Native and redesigned proteins exhibited highly similar characteristics, predicted structures and binding pockets, and slightly different docking poses against known ligands, although task-specific designs are still required for proteins more susceptible to internal hydrogen bond formations. (mit.edu)

Postdiagnosis dietary factors, supplement use and breast cancer prognosis: Global Cancer Update Programme (CUP Global) systematic literature review and meta-analysis. (who.int)
Postdiagnosis recreational physical activity and breast cancer prognosis: Global Cancer Update Programme (CUP Global) systematic literature review and meta-analysis. (who.int)
Postdiagnosis body fatness, weight change and breast cancer prognosis: Global Cancer Update Program (CUP global) systematic literature review and meta-analysis. (who.int)
Physical Activity and Health-Related Quality of Life in Women With Breast Cancer: A Meta-Analysis. (who.int)
Metabolomic profiles of metformin in breast cancer survivors: a pooled analysis of plasmas from two randomized placebo-controlled trials. (who.int)

Supplementary material for the article describing the database and the analysis behind of that is available from here. (lu.se)

The study would benefit from a broader analysis of gut microbiota proteome and further characterization of B cells infiltrating the liver tissue including their numbers/field and their origin (infiltrating versus resident cells). (elifesciences.org)
Molecular characterization of the immune system: emergence of proteins, processes, and domains. (lu.se)

Marked differences in protein expression were observed that are driven by histotype. (biomedcentral.com)
Scope includes mutations and abnormal protein expression. (cancerindex.org)
Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. (cancerindex.org)
Though Affy and Illumina's overall businesses are different, with Illumina a major player in the next-generation sequencing market and Affy, following the recent acquisition of eBioscience, among the larger providers of flow cytometry reagents, both Witney and Flatley portrayed the dynamic facing their array businesses in a similar way, with growth in applied markets offsetting declines in mature research markets, such as GWAS and expression array studies. (genomeweb.com)
While that may signal a continued decrease in whole-genome genotyping and whole-genome expression array-driven studies, historically among the most lucrative array market segments for both firms, Flatley and Witney portrayed their array businesses as shifting in new and profitable directions. (genomeweb.com)
Here, we aim to identify the PP1, PP8, and pp22 and protein expression patterns placenta and trophoblast them to reveal the involvement of the potential complications of pregnancy. (microarraystation.com)
The two strains mediate adhesion by different mechanisms and, when combined, synergically induce the expression of Caco-2 tight junction proteins. (frontiersin.org)
By using pooled Protein from whole tissues you can get better protein representation, homogenous signal intensities, stable expression profiles, and high reproducibility between different lots. (biochain.com)
OSCC patients with high IL-6 expression showed a poor prognosis by Kaplan-Meier survival analysis. (bvsalud.org)
Multivariate Cox regression analysis showed that high expression of IL-6 was an independent risk factor affecting the prognosis of patients with OSCC(Pï¼ 0.05). (bvsalud.org)
Spearman correlation analysis showed a positive correlation between IL-6 and ß-catenin expression in OSCC (Pï¼ 0.001). (bvsalud.org)

An important subclass of these motifs are characterized by a high proline content and play a pivotal role in biological processes requiring the coordinated assembly of multi-protein complexes. (torvergata.it)

For biomedical applications, we are combining rational design and combinatorial methods to create designer protein therapeutics and diagnostic agents. (stanford.edu)

Our study revealed for the first time that multiple invasion-related signaling proteins (β-catenin, caveolin, Src and EGFR), as well as several growth-related proteins (AMPKα, phospho-acetyl-CoA carboxylase (p-ACC), HER-2 and mTOR), which were previously un-described signaling proteins altered by YC-1, were found to be down-modulated by YC-1 in NPC cells. (elsevierpure.com)

Specifically, Flatley said that Illumina will integrate its cytogenetic chips with BlueGnome's BlueFuse analysis software. (genomeweb.com)

The aim of our group is to develop a sensitive technique for analyzing signals on protein microarray by applying the surface plasmon resonance (SPR) method. (nycu.edu.tw)

There is information in other parts of the genome and we can't capture that all on arrays and that will drive our sequencing business in a positive way and probably hurt our array business," said Flatley of the ENCODE findings. (genomeweb.com)

Here we identified Nup2 in a pool of enriched proteins that co-purify with tagged Ndj1 from meiotic cell extracts. (biorxiv.org)
Enrichment analysis for protein localization showed that mainly intracellular and cell-associated interacting proteins were identified. (degruyter.com)

Here we make available via a publicly accessible database a synopsis of our current understanding of the interaction landscape of the human SH3 protein family. (torvergata.it)
The "self"-protein albumin in mouse airway fluid was uniquely modified by GSH-MDI at position 414K, a preferred site of MDI reactivity on human albumin. (cdc.gov)

Therefore, we performed metabolic analyses of EBV-positive OSCC cells and established a xenograft model to investigate the viral contribution to OSCC progression. (bvsalud.org)

The methods for tissue fixation currently used in traditional histology are of limited use for molecular analysis. (qiagen.com)

At BioChain, we pool extracted Total Protein from a collection of high-quality tissue sources in order to provide the broadest coverage of proteins. (biochain.com)

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