Proteins nucleic acid hybridization. Medical search. Frequent questions

During the early 1990s, breakthroughs in cloning and sequencing of Norwalk virus and Southampton virus ( 15--18 ) led to the development of sensitive molecular assays (e.g., reverse transcription-polymerase chain reaction [RT-PCR]), nucleotide hybridization probes, and enzyme-linked immunosorbent assays (ELISA) that used baculovirus-expressed viral antigens ( 19--33 ). (cdc.gov)
The laboratory section will provide students with hands-on experimentations in major techniques in molecular biology such as DNA and RNA isolation, protein purification, DNA and protein electrophoresis, nucleic acid hybridization and polymerase chain reaction. (aucegypt.edu)

M. genitalium, a humanurogenital pathogen, is the extreme manifestation of this genomicparsimony, having only 482 protein-coding genes and the smallest genomeat -580 kb of any known free-living organism capable of being grown inpure culture(13). (fullblog.com.ar)
The1999 report by some of the present inventors on the essential microbialgene for M. genitalium and its closest relative, Mycoplasma pneumoniae,mapped ~2200 transposon insertion sites in these two species, andidentified 130 putatively non-essential M. genitalium protein-codinggenes or M. pneumoniae orthologs of M. genitalium genes. (fullblog.com.ar)
1999) Science 286, 2165-9), those authors estimatedthat 265 to 3,50 of the protein-coding genes of M. genitalium areessential under laboratory growth conditions(4). (fullblog.com.ar)
Ribonucleic acid (RNA) has multiple roles in cellular functions, from coding genes to non-coding regulatory activities. (biomedcentral.com)
Most yeast ribosomal protein genes are duplicated and their characterization has led to hypotheses regarding the existence of specialized ribosomes with different subunit composition or specifically-tailored functions. (prolekarniky.cz)
In yeast, ribosomal protein genes are generally duplicated and evidence has emerged that paralogs might have specific roles. (prolekarniky.cz)
A growing number of human diseases have been linked to mutations in genes encoding factors involved in ribosome biogenesis and protein synthesis [5] , [6] . (prolekarniky.cz)
These nucleic acid polymers encode for the all of the materials an organism needs to live in the form of genes. (wikibooks.org)
Genes are small blocks of DNA that tell the cell which proteins it should create. (wikibooks.org)
Multispectral confocal microscopy and CODEX imaging of genes, transcripts, and proteins as well as single-cell genomic analysis, and spatial transcriptomics are routinely utilized. (uvm.edu)
all genes associated with this syndrome (ie, DKC1 , TERT, TERC, NOP10 ) encode proteins in the telomerase complex responsible for maintaining telomeres at the ends of chromosomes regarding shortening length, protection, and replication. (medscape.com)

18. What nucleic acid does in situ hybridization stain? (examyear.com)
19. When used in in situ hybridization, RNA probes are _______ to the sample's RNA. (examyear.com)
In situ hybridization histochemistry and the study of gene expression in the human brain. (ox.ac.uk)
HCR is a molecular mechanism for in vitro and in situ amplification of signals to spatially identify proteins, RNA, or DNA in a sample. (caltech.edu)
In vivo expression of mRNA for the Ca++-binding protein SPARC (osteonectin) revealed by in situ hybridization. (ox.ac.uk)
In situ hybridization is used to survey the tissue-specific and developmental expression of the cloned mouse gene Sparc, coding for a protein homologous to the bovine Ca++-binding protein, osteonectin. (ox.ac.uk)
A short introduction how cytogenetics and molecular cytogenetics were established is followed by technical aspects of fluorescence in situ hybridization (FISH). (frontiersin.org)
This review is about "molecular cytogenetics" including 1) the historical perspective of its development from cytogenetics, 2) technical aspects, 3) available probe sets, and 4) variants and applications of the basic fluorescence in situ hybridization (FISH) approach. (frontiersin.org)
Importantly, vaccinated animals were protected against intranasal challenge with IDV [3 x 10(5) 50% tissue culture infective dose(s) (TCID50)] D/OK (n = 6) or D/600 (n = 6), based on the absence of viral RNA in necropsied tissues (5 and 7 days postchallenge) using quantitative reverse transcription-PCR and in situ hybridization. (cdc.gov)

5' - & 3' - End labeling of nucleic acids, Sequencing of nucleic acids, Nucleic acid hybridization, Making of labelled hybridization probes, Gene cloning, Studies on mechanism of DNA repair and DNA replication, Transcription of genetic message to RNA, Translation of genetic information from RNA to protein. (britatom.gov.in)
Detecting gamma-irradiated SARS-CoV-2 virions in an extraction buffer, the current amplified HCR lateral flow assay achieves a limit of detection of 200 copies/µL using nucleic acid probes to target the SARS-CoV-2 RNA genome. (caltech.edu)
By swapping out nucleic acid probes to target different pathogens, amplified HCR lateral flow assays offer a platform for adaptable and sensitive at-home testing for emergent diseases. (caltech.edu)
Based on the knowledge and tools developed while identifying and characterizing new viruses and comparisons to previously-characterized viruses, new virus-specific and broad spectrum polyclonal and/or monoclonal antibody reagents, purification protocols, nucleic acid hybridization probes, PCR primers, isothermal amplification methods, and improved associated protocols will be developed. (usda.gov)

Protein Analysis: Protein gel electrophoresis allows researchers to separate proteins based on size and charge. (excedr.com)
Techniques like sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) denature proteins and provide a uniform charge-to-mass ratio, facilitating the comparison of protein sizes. (excedr.com)
Gel electrophoresis is also used to analyze post-translational modifications and protein complexes. (excedr.com)

UPF0687 protein C20orf27 is a protein that in humans is encoded by the C20orf27 gene. (wikipedia.org)
What does this gene/protein do? (cancerindex.org)
proposed a 206 protein-coding gene core of aminimal bacterial gene set based on analysis of several free-living andendosymbiotic bacterial genomes (3). (fullblog.com.ar)
The coding region of the carboxy- terminal half of polyhedrin was removed and the remainder was ligated with the IGF II gene in phase to create a fusion protein gene consisting of the coding region of the amino-terminal half of polyhedrin and the IGF II gene. (microbiologyresearch.org)
This fusion protein gene was combined in a plasmid with the promoter and 5′ and 3′ flanking regions of the polyhedrin gene. (microbiologyresearch.org)
Recent evidence suggests that a mutation in the spike protein gene of feline coronavirus (FCoV), which results in an amino acid change from methionine to leucine at position 1058, may be associated with feline infectious peritonitis (FIP). (biomedcentral.com)
Unlike yeast, most mammalian ribosomal proteins are thought to be encoded by a single gene copy, raising the possibility that heterogenous populations of ribosomes are unique to yeast. (prolekarniky.cz)
Nucleic acid labeling and hybridization on membranes have formed the basis for a range of experimental techniques involving understanding of gene expression, organization, etc. (teachntest.org)
This pattern of differential gene expression demands a reassessment of the function originally proposed for osteonectin, and predicts a much wider role for the protein in a variety of biological processes. (ox.ac.uk)
In addition, to the passage of information from gene to protein will be addressed. (aucegypt.edu)
This course introduces students to the different aspects of the biotechnology revolution including principles of recombinant DNA technology, protein engineering, directed mutagenesis, manipulation of gene expression, microbial synthesis of biologics, biomass utilization, large scale production of proteins, transgenic animals, and the human genome project. (aucegypt.edu)
In the X-linked recessive form, the gene defect lies in the DKC1 gene (located at Xq28), which encodes for the protein dyskerin. (medscape.com)
Another gene implicated in DKC, TINF2 , encodes a key component of the protein shelterin, which plays a role in telomere homeostasis. (medscape.com)

Grace Bio-Labs HybriWell ™ sealing system seals securely to a microscope slide surface in seconds to accommodate waterbath hybridizations, such as protein or nucleic acid hybridization assays. (sigmaaldrich.com)
By comparison, five commercial unamplified lateral flow assays that use proprietary antibodies to target the viral nucleocapsid protein exhibit limits of detection of 500 copies/µL, 1000 copies/µL, 2000 copies/µL, 2000 copies/µL, and 20,000 copies/µL. (caltech.edu)

Here, we enhance sensitivity by developing an amplified lateral flow assay that incorporates isothermal, enzyme-free signal amplification based on the mechanism of hybridization chain reaction (HCR). (caltech.edu)

RNA codons and protein synthesis. (crossref.org)
The most persuasive roles for HSV and HCMV in human tumourigenesis are as mutagens, as activators of cellular transcription or in switching on the synthesis of host cell proteins not normally expressed in untransformed cells. (microbiologyresearch.org)
For example, it can have inhibitory effects on glycolysis followed by a direct effect on protein synthesis [ 2 ], as well as increase the absorption and retention of calcium, magnesium and phosphate ions in the body. (biomedcentral.com)
Protein synthesis is a major energy consuming process involving intricate coordination of translation machinery in response to nutrient availability and stress sensing signals, as well as hormonal and growth factor cues in multi-cellular organisms. (prolekarniky.cz)
Ribosome synthesis is a highly controlled process, whereby three distinct RNA polymerases are synchronously coordinated to produce equimolar amounts of four rRNAs and 79 mammalian ribosomal proteins (RPs) [1] - [4] . (prolekarniky.cz)
An exemplary method involves detection of single molecules of fluorescent labels released from a nucleic acid during synthesis of an oligonucleotide. (justia.com)
Evidence exists for telomerase dysfunction, ribosome deficiency, and protein synthesis dysfunction in this disorder. (medscape.com)

The prefix "deoxy" distinguishes DNA from its close relative RNA (ribonucleic acid). (wikibooks.org)
Nucleotides are obtained on hydrolysis of deoxyribonucleic acid (DNA) and ribonucleic acids (RNA) under controlled conditions. (britatom.gov.in)

Antibodies or immunoglobulins are a class of disease-fighting proteins produced by B cells found in the lymphatic system or bone marrow. (teachntest.org)

1). Protein purification a. (ntu.edu.tw)
Benchmark's new Roto-Mini series of rotating mixers provides a wide range of mixing capabilities, suitable for an array of laboratory applications including resuspension, protein binding, nucleic acid purification, histochemistry, hybridization etc. (pathtech.com.au)

IGF II was released from the fusion protein produced by BM-N cells infected with the recombinant virus by CNBr treatment, purified by extraction with guanidine-HCl, column chromatography and HPLC and the correct amino-terminal amino acid sequence confirmed. (microbiologyresearch.org)
has shown that it is possible to distinguish between FCoVs from animals with and without FIP on the basis of one or more substitutions in the amino acid sequence that comprises the furin cleavage motif within the S protein [ 15 ]. (biomedcentral.com)

Cells infected with this recombinant virus (gG-VAC) synthesized glycosylated proteins of 48K, 57K and 61K mol. (microbiologyresearch.org)
The silkworm larvae infected with the recombinant virus produced 3·6 mg of the fusion protein per larva and the infected BM-N cells produced 0·3 mg per ml of culture. (microbiologyresearch.org)
The production of recombinant mature hormone in suitable hosts and secretion of this therapeutic protein into the extracellular space can be considered as one of the best cost-effective approaches not only to obtain the active form of the protein but also endotoxin-free preparation. (biomedcentral.com)
On the other hand, recombinant therapeutical proteins have received a great deal of attention in recent years due to their advantages including low side effects, minimized cytotoxicity, high selectivity, and very low non-specific interactions [ 7 , 8 ]. (biomedcentral.com)

Of particular value are methods for measuring small quantities of nucleic acids, peptides, saccharides, pharmaceuticals, metabolites, microorganisms and other materials of diagnostic value. (justia.com)

Morphological transformation studies have failed to demonstrate a viral oncogene, a virus-coded transforming protein or any sequence of DNA that uniquely transforms cells according to one-hit kinetics. (microbiologyresearch.org)
The S protein fusion peptide is a critical element in the fusion of viral and cellular membranes during virus entry [ 14 ] and it is reasonable to think that amino acid substitutions within this peptide may alter the tropism of the virus. (biomedcentral.com)
Plant Dicer-like proteins (DCLs) are RNase III, double-stranded RNA (dsRNA)-specific endonucleases with specialized functions in producing short (s)RNAs of 21- to 24-nucleotides (nt), including micro (mi)RNAs and short interfering (si)RNAs of endogenous or viral origin. (biomedcentral.com)
In particular, as RNA binding proteins, RPs have been found to bind cellular and viral RNAs outside of the context of the ribosome. (prolekarniky.cz)

The invention also relates to improved methods for sequencing and genotyping nucleic acid in a single molecule configuration. (justia.com)
Next generation sequencing (NGS) of nucleic acid extracts from plants infected with unknown viruses is expected to yield information about the genomes of previously uncharacterized viruses without any background information on what viruses might be infecting the plant. (usda.gov)

A Western Blot binding pattern on protein C20orf27 with its polyclonal antibody reveals that the experimental molecular weight of protein C20orf27 is about 22 kDa. (wikipedia.org)
In this study, using a combination of the bioinformatics design and molecular genetics, the protein A signal peptide from Staphylococcus aureus was modified, redesigned and then fused to the mature hGH coding region. (biomedcentral.com)
These features of nucleic acids enable us to design novel interaction pathways and functions by providing well-behaved molecular mechanisms. (caltech.edu)
This thesis will first demonstrate the use of these nucleic acid molecular mechanisms in closed-loop genetic circuits and infectious disease testing using cgRNAs and HCR, respectively, then provide updated tools for the nucleic acid design community to exploit the programmable nature of nucleic acids. (caltech.edu)
NUPACK is a growing software suite for the analysis and design of nucleic acid structures, devices, and systems serving the needs of researchers in the fields of nucleic acid nanotechnology, molecular programming, synthetic biology, and across the life sciences. (caltech.edu)

Because of the presence of an upstream ORF that is predicted to interfere with translation of this variant, the transcription variant 4 does not encode any protein. (wikipedia.org)

6). Detection of specific nucleic acid sequences by hybridization a. mini-prep of plasmid DNA b. (ntu.edu.tw)
We then used the hybridization chain reaction mechanism to develop an adaptable and sensitive test for the detection of SARS-CoV-2. (caltech.edu)

MALDI-TOF utilizes mass spectrometry and "identifies bacteria based on unique protein sequences. (contagionlive.com)

Interstitial retinoid-binding protein (IRBP) is synthesized and secreted by rod photoreceptor cells into the interphotoreceptor matrix and is known to bind retinoids and fatty acids. (utah.edu)

We show that this united ExM (uniExM) protocol can be used to preserve and visualize RNA transcripts, proteins in biologically relevant ultrastructures, and sets of RNA transcripts in patient-derived xenograft (PDX) cancer tissues and may support the visualization of other kinds of biomolecular species as well. (plos.org)
Nucleic acid nanotechnologies have provided a platform where biologically relevant molecules can be engineered to perform programmable functions. (caltech.edu)

DNA stands for deoxyribonucleic Acid . (wikibooks.org)

Colony hybridization b. (ntu.edu.tw)

Nucleotides are the basic unit of nucleic acids just as aminoacids are the basic unit of proteins and monosaccharides (carbohydrates) are of polysaccharides (sugars). (britatom.gov.in)

Recent advances in genetic engineering have made it possible for microorganisms to be used for expression of heterologous proteins. (biomedcentral.com)
In this approach, heterologous proteins are expressed either as cytoplasmic or intracellularly or secreted as extracellularly [ 11 ]. (biomedcentral.com)

Bacterial artificial chromosome-comparative genomic hybridization (BAC-CGH) has been used to look for genomic amplifications and deletions which may also contribute to expression changes. (ox.ac.uk)

Two examples of these mechanisms are the conditional guide RNA (cgRNA) and the hybridization chain reaction (HCR). (caltech.edu)

In plants, RNase III Dicer-like proteins (DCLs) act as sensors of dsRNAs and process them into short 21- to 24-nucleotide (nt) (s)RNAs. (biomedcentral.com)

The p63 protein, a homologue of p53, may be associated with tumor formation in the epithelial tissue, acting as an oncogene 11,12 . (bvsalud.org)

An alternative approach to the identification of the murine homologue was to use cross-species DNA hybridization, employing human CD2 cDNA as a probe. (ox.ac.uk)
The murine cDNA sequence encoded a predicted mature polypeptide of 322 amino acids that showed 54% identity with the predicted human sequence. (ox.ac.uk)

However, protein components of genetic circuits cannot be designed scalably, are often mined from preexisting genomes, and present difficulties in being biologically orthogonal to themselves or the host organism. (caltech.edu)

Scope includes mutations and abnormal protein expression. (cancerindex.org)

DNA is a long chain of linear polymers containing deoxyribose sugars and their covalently bonded bases known as nucleic acids. (wikibooks.org)
volume = {285}, number = {32}, pages = {24307--24312}, abstract = {Inhibitory serpins are metastable proteins that undergo a substantial conformational rearrangement to covalently trap target peptidases. (cnrs.fr)

This protein is expected to be found in cytosol and nucleus, but not in nuclei. (wikipedia.org)
Microarray-assessed tissue expression pattern suggests caudate nucleus has the highest expression of protein C20orf27. (wikipedia.org)

Transcript variant 1 encodes for the longest protein isoform, with a size of 1327 bases and 6 exons. (wikipedia.org)
Exceptional compatibility with acids, bases, and oxidizer. (aijirentech.com)
In DNA a sequence of three bases, which is called a codon, is responsible for the encoding of a single amino acid. (wikibooks.org)

Laboratory and bioinformatic investigations identified and validated 9 genetically related SARS-CoV-2 viruses with a hybrid Delta-Omicron spike protein. (cdc.gov)

There are many predicted phosphorylation sites along the sequence of protein C20orf27, including four sites for protein kinase A (PKA), two sites for protein kinase C (PKC), three sites for casein kinase 2 (CKII), one site for ribosomal S6 kinase (RSK), one site for cGMP-dependent protein kinase or Protein Kinase G (PKG), and one site for ataxia-telangiectasia mutated (ATM) serine/threonine protein kinase. (wikipedia.org)

Protein C20orf27 has no high scoring hydrophobic regions, no highly charged regions, and no transmembrane regions. (wikipedia.org)

In addition, interventions leading to reduced translation, such as dietary restriction and reduced 60S ribosomal protein expression, elicits lifespan extension in yeast, worms and files [10] - [13] . (prolekarniky.cz)
5). Protein expression system a. (ntu.edu.tw)
CONCLUSION: This indicates that epigenetic changes affect the expression of the Smad4 protein in prostate cancer and points to methylation of the promoter as a novel marker of and contributor to the disease warranting further study. (ox.ac.uk)

BmNPV genomic DNA codes polyhedrin which is a major protein of inclusion bodies and is mass- produced in infected silkworm larvae. (microbiologyresearch.org)

The Ribosomal Protein Rpl22 Controls Ribosome. (prolekarniky.cz)
Consistent with the hypothesis that either ribosomal protein can support translation, knockdown of Rpl22l1 impairs growth of cells lacking Rpl22 . (prolekarniky.cz)
We propose that ribosome specificity may exist in mammals, providing evidence that one ribosomal protein can influence composition of the ribosome by regulating its own paralog. (prolekarniky.cz)
Dyskerin is composed of 514 amino acids and has a role in ribosomal RNA processing and telomere maintenance. (medscape.com)

Cystatin A (Cys A), a cysteine protease inhibitor, is a precursor of proteins involves in keratinocyte keratinization, and is expressed during the late phase of differentiation of these cells. (bvsalud.org)

A second substitution, two amino acids downstream of M1058L (serine to alanine at position 1060, S1060A) distinguished a further 4% of FIP from non-FIP associated FCoVs. (biomedcentral.com)

Since the native signal protein peptide of S. aureus protein A was not able to deliver hGH to the extracellular space, it was modified using bioinformatics tools and fused to the n-terminal region of hGh to show that the redesigned signal peptide was functional. (biomedcentral.com)
Interphotoreceptor retinoid-binding protein (IRBP) is an extracellular protein that has been suggested to participate in the visual process as a carrier for visual retinoids. (utah.edu)

The first repetitive structure is amino acid alphabet structures with a core block length of 4. (wikipedia.org)

Anatomy1

Organisms4

Chemicals and Drugs22

Analytical, Diagnostic and Therapeutic Techniques and Equipment14

Phenomena and Processes15

Information Science4

Health Care3

Polymerase Chain Re2

Genes11

Situ9

Probes4

Electrophoresis3

Gene13

Assays2

Amplification1

Synthesis7

Ribonucleic2

Antibodies1

Purification2

Amino acid seq2

Recombinant4

Peptides1

Viral4

Sequencing2

Molecular5

Encode1

Detection2

Sequences1

Fatty acids1

Biologically relevant2

Deoxyribonucleic acid1

Colony1

Carbohydrates1

Heterologous2

Bacterial1

Mechanisms1

Plants1

Tumor1

CDNA2

Genetic1

Abnormal protein expression1

Covalently2

Nucleus2

Bases3

Laboratory1

Sequence1

Regions1

Expression3

Genomic1

Ribosomal4

Precursor1

Serine1

Extracellular2

Structures1