Proteins lipid peroxidation. Medical search. Frequent questions

Furthermore, LA can scavenge lipid peroxidation products such as hydroxynonenal and acrolein. (weeksmd.com)

The chemical products of this oxidation are known as lipid peroxides or lipid oxidation products (LOPs). (wikipedia.org)
Recently it has been found that in cereals, cold stress protein CSP 310 exists, and that this causes uncoupling of oxidation and phosphorylation in mitochondria. (biomedcentral.com)
mtROS also causes oxidation of lipids and proteins identified in bleomycininduced mouse models of pulmonary fibrosis and in sufferers with IPF (80, 81). (namptinhibitor.com)
Antioxidant enzyme expression, lipid peroxidation, and protein oxidation in human myometrium with parturition Reproductive Sciences. (nottingham.ac.uk)

Immunoblotting of protein extracts from luteal tissue revealed three major reactive bands. (johnshopkins.edu)
The end products of lipid peroxidation are reactive aldehydes, such as malondialdehyde (MDA) and 4-hydroxynonenal (HNE), the second one being known also as "second messenger of free radicals" and major bioactive marker of lipid peroxidation, due to its numerous biological activities resembling activities of reactive oxygen h species. (wikipedia.org)
Cross-linking between cysteine and lysine, tryptophan or tyrosine in peptides and proteins treated with hypochlorous acid and other reactive halogens. (otago.ac.nz)
Our results revealed that H2O2 increased the rate of apoptosis by stimulating various pro-apoptotic processes, such as the generation of intracellular reactive oxygen species (ROS), lipid peroxidation, DNA fragmentation and caspase-3 activation. (spandidos-publications.com)
Scientists are therefore keenly interested in finding ways to reduce lipid peroxidation by lowering LDL levels directly and by reducing the rate at which these molecules become damaged by reactive oxygen species. (lifeextension.com)
The basis of hapten- molecular mechanisms of the sensitization pro- protein binding work is the hypothesis that upon cess will result in novel opportunities for the skin absorption, only protein-reactive chemicals development of alternative methods for assessing (or those that can be metabolically or chemically skin sensitization hazard and relative potency of converted to protein-reactive species) are able to chemicals. (cdc.gov)
In lung tissue, lipid peroxidation, reactive oxygen species, superoxide dismutase activity and reduced glutathione were all increased by WPS exposure. (who.int)

Antioxidants such as vitamin C and vitamin E may inhibit lipid peroxidation. (wikipedia.org)
Additionally, a significant depletion of antioxidants, accumulation of oxidatively modified proteins and an increase in lipid peroxidation products were found in the cells exposed to CNC. (cdc.gov)
Comparative study on the influence of fluoride on lipid peroxidation and antioxidants levels in the different brain regions of well-fed and protein undernourished rats. (fluoridealert.org)

It was shown to inhibit lipid peroxidation and UV-induced crosslinking of collagen. (sigmaaldrich.com)

The present study had evaluated the protective effect of hydroalcoholic (50-50: v/v) and aqueous extracts of L. kerstingii and M. oleifera against lipid peroxidation induced in vivo and in vitro by either cadmium or ethanol. (scialert.net)
Fusion proteins also possess GST -enzymatic activity and can undergo dimerization similar to in vivo. (prospecbio.com)

Burbach, J. A. / Localization of lipid peroxidation-derived protein epitopes in the porcine corpus luteum . (johnshopkins.edu)

The large proteoglycan molecules made up of protein cores, and GAG branches are secreted by cells and constitute a significant fraction of the extracellular matrix of connective tissue. (medscape.com)
Advanced glycation is a major pathway for the posttranslational modification of plasma and tissue proteins. (iospress.com)
Mn, serum Mn, urine Mn, Centromere-positive micronuclei in periphery blood lymphocytes, DNA protein-crosslinks in peripheral white blood cells, oxidative stress as urinary 8OHdG, and long-term oxidative damage measured as erythrocytic superoxide dismutase (SOD) activity (measure of systemic oxidative stress) and serum malondialdehyde (MDA) (product of lipid peroxidation and reflect tissue injuries). (who.int)

01). We speculate that perivascular lipid/protein modification of the diaphragm in sepsis results from the action of neutrophil-derived free radicals, while myocyte-derived radicals cause NT/4HN formation along the muscle perimeter. (uky.edu)
It is the process in which free radicals "steal" electrons from the lipids in cell membranes, resulting in cell damage. (wikipedia.org)
Free radicals are responsible for lipid peroxidation and have received much attention recently in connection with a variety of pathologies such as cancer (Zhu et al . (scialert.net)
Comprising both lipid and proteins, LDL particles are particularly vulnerable to the effects of oxygen free radicals. (lifeextension.com)
Lipid peroxidation of cell membranes induced by free radicals may contribute to preeclampsia. (msdmanuals.com)

Copper ions bind to biomolecules (e.g., peptides and proteins) playing an essential role in many biological and physiological pathways in the human body. (mdpi.com)

S. Slathia, A. Sharma and S. Choudhary, "Influence of Exogenously Applied Epibrassinolide and Putrescine on Protein Content, Antioxidant Enzymes and Lipid Peroxidation in Lycopersicon esculentum under Salinity Stress," American Journal of Plant Sciences , Vol. 3 No. 6, 2012, pp. 714-720. (scirp.org)

His current projects involve using mass spectrometry to characterise oxidative modifications to the neutrophil protein calprotectin that may be useful as biomarkers of inflammation, and to identify proteins that are being oxidatively-modified during the early stages of necroptosis. (otago.ac.nz)

We assessed motor alterations, nitrosative stress, lipid peroxidation, and the antioxidant system comprised of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx). (hindawi.com)

Lipid peroxidation is the chain of reactions of oxidative degradation of lipids. (wikipedia.org)
For apoptotic inducing activity, human osteogenic sarcoma (HOS) cell proliferation, chromosomal DNA degradation and changes in apoptosis-related protein levels were measured. (nutrimedical.com)
The fact that cycloheximide delays tepal senescence demonstrates the synthesis of particular proteins probably enzymes, responsible for degradation of cellular constituents, executes the cell death programme in flower tepals. (scialert.net)

This review summarizes evidence for the role of proteolytic enzymes that degrade and inactivate insulin-like growth factor binding proteins (IGFBP) during follicular development in mammals. (bioone.org)

Cadmium a very toxic and ubiquitous metal and ethanol (95%) are known to induce lipid peroxidation (Traore et al . (scialert.net)

The expression of transthyretin, a protein shown to sequester Aβ and prevent amyloid fibril formation in vitro , and several genes in the insulin-signaling pathway, e.g., insulin-like growth factor-2, were increased selectively in the hippocampus of APP Sw mice. (jneurosci.org)
Thus, the principle of protein or peptide haptenation could be used in in vitro assays to predict the sensitization potential of a new chemical entity. (cdc.gov)

At least two of these bands were closely associated with extracted oxidized human low-density lipoprotein (LDL) and with 4-HNE-conjugated human apoprotein B. These results demonstrate the presence of lipid peroxidation-derived protein epitopes within the porcine CL and show that one or more of these may be an oxidized. (johnshopkins.edu)
Using data from the second phase (1991-1994) of the Third National Health and Nutrition Examination Survey (NHANES III), we examined associations between 22 polymorphisms in 13 candidate genes and four serum lipids: high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), total cholesterol (TC), and triglycerides (TG). (cdc.gov)
Results from our study contribute to a growing body of literature identifying key determinants of plasma lipoprotein concentrations and could provide insight into the biological mechanisms underlying serum lipid and cholesterol concentrations. (cdc.gov)
The genetic basis for elevation in lipid levels is not well understood, but substantial heritability has been demonstrated in twin [ 9 ] and family-based [ 10 - 12 ] studies, which have estimated that approximately 43% to 83% of the variance in blood lipid and lipoprotein levels is attributable to genetic factors. (cdc.gov)

In recent years, development of immunochemical detection of HNE-histidine adducts opened more advanced methodological possibilities for qualitative and quantitative detection of lipid peroxidation in various human and animal tissues[full citation needed] as well as in body fluids, including human serum and plasma samples. (wikipedia.org)
LDL transports lipids (fats) from the digestive system to tissues. (lifeextension.com)

Unsaturated lipids are susceptible to peroxidation by ROS, leading to the formation of toxic aldehydes such as malondialdehyde (MDA) and 4-hydroxy-nonenal (4-HNE) that can attack cellular and extracellular proteins. (johnshopkins.edu)
Previous results demonstrated that ALDH3A1 confers resistance against UV- and 4-hydroxynonenal-induced a poptosis, mainly by metabolising toxic lipid peroxidation aldehydes. (europa.eu)

The toxicity of lipid hydroperoxides to animals is best illustrated by the lethal phenotype of glutathione peroxidase 4 (GPX4) knockout mice. (wikipedia.org)
These animals do not survive past embryonic day 8, indicating that the removal of lipid hydroperoxides is essential for mammalian life. (wikipedia.org)
GST reduces lipid hydroperoxides through its Se-independent glutathione peroxidase activity. (prospecbio.com)

Alternatively, indirect methods for assessing oxidative stress have been applied and focus mainly on the oxidative damage ROS cause in biomolecules, such are lipids, proteins, and DNA. (molvis.org)

This represents the first direct in situ evidence that protein modification by lipid peroxidation products occurs during the natural luteal life span. (johnshopkins.edu)
In addition, end-products of lipid peroxidation may be mutagenic and carcinogenic. (wikipedia.org)
Certain diagnostic tests are available for the quantification of the end-products of lipid peroxidation, to be specific, malondialdehyde (MDA). (wikipedia.org)
Damage of DNA and proteins by major lipid peroxidation products in genome stability. (nih.gov)
2004. Vitamin C conjugates of genotoxic lipid peroxidation products: structural characterization and detection in human plasma. . (oregonstate.edu)
The enzyme also detoxifies lipid peroxidation end products such as 4-hydroxynonenal (4-HNE). (prospecbio.com)

Concomitant activation of the insulin-like growth factor-1 receptor, Akt, and extracellular signal-regulated protein kinase 1 and 2 as well as increased phosphorylation of Bad also were unique to the hippocampus of APP Sw mice. (jneurosci.org)
In addition, we confirmed that H2O2 activated mitogen-activated protein kinases (MAPKs), such as extracellular signal-regulated kinase (ERK), c-Jun NH2-terminal kinase (JNK) and p38 MAPK. (spandidos-publications.com)

Moreover, the findings of the present study establish the use of protein carbonyls (together with a methodology for their more accurate quantification, which overcomes serious unreliability problems of past methods) as an age accumulative marker of OS damage, for future studies that investigate long-term OS involvement in pseudoexfoliative ocular disorders. (molvis.org)

Cell viability was examined by MTT assay and lipid peroxidation was assayed by thiobarbituric acid (TBA) reaction. (spandidos-publications.com)

However, there are other sources of malondialdehyde, so this test is not completely specific for lipid peroxidation. (wikipedia.org)
Peroxide and malondialdehyde levels are often increased by freezing and thawing stress suggesting peroxidation of lipids [ 2 ] that result in structural and functional membrane and membrane protein changes [ 3 ]. (biomedcentral.com)

At the same time, it is known that plant uncoupling mitochondrial protein (PUMP) and other UCP-like proteins are not the only uncoupling system in plant mitochondria. (biomedcentral.com)
The levels of mitochondrial protein acetylation were increased in the hippocampus of HFD group. (hindawi.com)

We studied the effects of CSP 310-like native cytoplasmic proteins from a number of cereal species (winter rye, winter wheat, Elymus and maize) on the energetic activity of winter wheat mitochondria. (biomedcentral.com)
A strong activation of cells resulted in the high number of cytoplasmic vacuoles, finger-like protrusions, lipid droplets and multi-nucleation. (cdc.gov)

Previous mouse studies suggest that electronic cigarette exposure plus a Western diet triggers oxidative stress leading to increased lipid peroxidation and hepatic steatosis, according to Dr. Theodore Friedman of Charles R. Drew University of Medicine & Science in Los Angeles. (medscape.com)
In liver samples, they observed that e-cigarette exposure led to hepatic lipid accumulation and oxidative stress, as well as a significant increase in hepatic expression of p53, a known inducer of insulin resistance and NAFLD. (medscape.com)

The initiating reaction is the nonenzymatic addition of sugars such as glucose to the primary amino groups of proteins, i.e., mainly to lysine residues. (iospress.com)

Decades of research have demonstrated that serum concentrations of blood lipids are associated with increased risk for cardiovascular disease and mortality [ 1 - 4 ]. (cdc.gov)

However, more reliable are those oxidative modifications that are not repairable and thus, accumulated, with protein carbonylation the most representative. (molvis.org)

Thus, they are able to inactivate sensitive proteins through electrophilic stress. (wikipedia.org)

The soluble GST is a 26 kDa protein which occurs as a dimer in all aerobic organisms. (prospecbio.com)

Tg2576 mice overexpress a mutant form of human amyloid precursor protein with the Swedish mutation (APP Sw ), resulting in high β-amyloid (Aβ) levels in the brain. (jneurosci.org)
For instance, familial AD is linked to mutations in the amyloid precursor protein (APP), presenilin 1, and presenilin 2, all of which lead to increased levels of Aβ. (jneurosci.org)
All recognized mutations for AD are associated with increased deposition of amyloid-beta (Abeta), a peptide fragment comprising 39-43 amino acids that derive from the catabolism of the amyloid precursor protein (APP) molecule. (medscape.com)

Microchromatographic analysis of lipids, protein, and occurrence of lipid peroxidation in various brain areas of vanadium exposed rats: a possible mechanism of vanadium neurotoxicity. (thieme-connect.com)

One of the most prominent stimuli of asthma, environmental things, can bring about damage to certain chain-complex proteins, sustaining ROS generation, and may further bring about airway hyperresponsiveness (AHR) (85, 86). (namptinhibitor.com)
The present study shows for the first time an age-increased OS-induced protein damage (protein carbonyl formation) in the AH and LECs/capsule of CAT patients with PEX, POAG or PXG. (molvis.org)
Diagnosis is by measuring blood pressure and urine protein and by tests to evaluate for end-organ damage (eg, pulmonary edema, impaired liver or kidney function). (msdmanuals.com)

Lysosomal function is critical for organismal homeostasis-mutations in genes encoding lysosomal proteins cause severe human disorders known as lysosomal storage diseases, and lysosome dysfunction is implicated in age-associated diseases including cancer, neurodegeneration and metabolic syndrome. (stanford.edu)
Many genes involved in metabolic pathways have been found to contribute to lipid level variability [ 14 , 26 , 27 ]. (cdc.gov)

On the other hand, it's unclear whether dietary lipid peroxides are bioavailable and play a role in disease, as a healthy human body has protective mechanisms in place against such hazards. (wikipedia.org)
Differential repair of etheno-DNA adducts by bacterial and human AlkB proteins. (nih.gov)
Briefly, they contain the human APP 695 with the double mutation K670N and M671L (Swedish mutation) and are driven by the prion protein promoter. (jneurosci.org)
This protease activity has recently been ascribed to serine metalloprotease(s), including pregnancy-associated plasma protein-A (PAPP-A), which was first detected in human follicular fluid nearly 20 yr ago. (bioone.org)
In human plasma, LA exists in an equilibrium of free and plasma protein bound form. (weeksmd.com)
In a study published in 2002, human volunteers consumed a lipid-rich meal, either with or without 300 mg of a proanthocyanidin-rich grape seed extract. (lifeextension.com)

No staining was observed in sections incubated with nonimmune mouse serum or when anti-4-HNE-lysine was preadsorbed with 4-HNE- protein preparations. (johnshopkins.edu)
The identification of genetic variants related to blood lipid levels within a large, population-based and nationally representative study might lead to a better understanding of the genetic contribution to serum lipid levels in the major race/ethnic groups in the U.S. population. (cdc.gov)

Chronic ER stress promotes apoptosis, at least in part through the UPR-induced transcription factor C/EBP homologous protein (CHOP). (jci.org)
These findings suggest that CHOP is a fundamental factor that links protein misfolding in the ER to oxidative stress and apoptosis in β cells under conditions of increased insulin demand. (jci.org)
Immunofluorescence staining, flow cytometry and western blot analysis were also used to determine apoptosis and the expression of proteins associated with apoptosis. (spandidos-publications.com)

Such D-PUFAs, for example, 11,11-D2-ethyl linoleate, suppress lipid peroxidation even at relatively low levels of incorporation into membranes. (wikipedia.org)
Pretreatment of murine myoblast (C2C12) cells with octyl-D-carnosine or carnosine enhanced HIF-1α protein expression, VEGF mRNA levels and VEGF release under hypoxic conditions. (frontiersin.org)
Recent candidate gene studies [ 13 - 16 ], as well as genome-wide association studies [ 17 - 25 ], have identified polymorphisms that account for a portion of the variation in blood lipid levels. (cdc.gov)

Oxidative stress ErbB4/HER4 Source causes lipid peroxidation, resulting in protein carbonylation, typically known as "carbonyl strain," that's predominantly associated with chronic ailments (76). (namptinhibitor.com)
In this cycle, carbonyl pressure can harm mitochondrial proteins and drive further endogenous production of ROS (69).Enhanced mtROS has been demonstrated in a quantity of fibrotic issues, such as pulmonary fibrosis. (namptinhibitor.com)
Average protein carbonyl concentrations were measured in patients grouped in 5-year age intervals (ranging from 56-60 to 86-90). (molvis.org)

Lipid peroxidation was determined using anti-4-hydroxynonenal (4HN) antibodies, protein nitrosylation was detected using anti-nitrotyrosine (NT) antibodies, and neutrophils were localized with a Wright's stain. (uky.edu)
Abcam: antibodies, proteins, kits. (abcam.com)

The lack of equilibrium within the brain structure and disorientation at the molecular/cellular level, neural interactions, and protein-protein interaction networks lead to cognitive dysfunction. (news-medical.net)

An alternative, pharmaceutical method employs the isotope effect on lipid peroxidation of deuterated polyunsaturated fatty acids (PUFAs) at the methylene bridges (bis-allylic sites) between double bonds, which leads to the inhibition of the chain reaction. (wikipedia.org)

Functional analysis showed that cholesterol biosynthesis pathways and the cytochrome 450 family of proteins (specifically Cyp4A10 that is associated with oxidative stress and lipid peroxidation leading to hepatocyte injury) were increased in mice exposed to e-cigarette aerosol. (medscape.com)

Aldehyde conjugation of proteins can have diverse effects on cell function, including cross-linking of cell surface receptors, alteration of enzyme activities, and modification of lipoproteins. (johnshopkins.edu)

2016. Xanthohumol improves dysfunctional glucose and lipid metabolism in diet-induced obese C57BL/6J mice. . (oregonstate.edu)

RESULTS: Ellagic acid showed high DPPH radical scavenging and lipid peroxidation inhibition activities. (nutrimedical.com)
Similar results were found in the kidney even though lipid peroxidation is slightly increased by ethanol in this organ. (scialert.net)

The process, known as lipid peroxidation, is a powerful stimulant of inflammation in blood vessels, eventually leading to plaque formation. (lifeextension.com)

A small rise in protein content was recorded under salinity stress in comparison with untreated control. (scirp.org)
The study of an influence of infiltration by different plant uncoupling system activators (pyruvate, which activates AOX, and linoleic acid which is a substrate and activator for PUMP and CSP 310) showed that all of these decreased lipid peroxidation during cold stress. (biomedcentral.com)

Variants within APOE (rs7412, rs429358), PON1 (rs854560), ITGB3 (rs5918), and NOS3 (rs2070744) were found to be associated with one or more blood lipids in at least one race/ethnic group in crude and adjusted analyses. (cdc.gov)
We report the significant associations of blood lipids with variants and haplotypes in APOE , ITGB3, NOS3 , and PON1 in the three main race/ethnic groups in the U.S. population using a large, nationally representative and population-based sample survey. (cdc.gov)

This showed that only CSP 310 (cold shock protein with molecular weight 310 kD) caused a significant increase of non-phosphorylative respiration. (biomedcentral.com)
The technique has been widely used to generate different kinds of proteins for crystallization, molecular immunology studies, the production of vaccines and studies involving protein-protein and protein-DNA interactions. (prospecbio.com)
Recombinant Schistosoma japonicum GST full length protein contains a total of 244 amino acids (1-218 a.a.) expressed in E.coli, having a molecular mass of 28.3kDa. (prospecbio.com)
One of the key molecular events in skin sensitization is protein haptenation, i.e. the chemical modification of self-skin protein(s) thus forming macromolecular immunogens. (cdc.gov)

In this review, we consider some of the theoretical aspects of protein haptenation, how mechanisms of protein haptenation can be investigated experimentally and how we can use such knowledge in the development of novel, alternative approaches for predicting skin sensitization potential in the future. (cdc.gov)

An increase in total sugar, phenolic and soluble protein content was observed with a concomitant decrease in α-amino acid content. (scialert.net)

Anatomy18

Organisms9

Diseases10

Chemicals and Drugs142

Analytical, Diagnostic and Therapeutic Techniques and Equipment18

Phenomena and Processes20

Technology, Industry, Agriculture6

Health Care2

Scavenge lipid peroxidation products1

Oxidation4

Reactive7

Antioxidants3

Inhibit1

Vivo2

Localization1

Tissue3

Free radicals5

Peptides and proteins1

Antioxidant Enzymes1

Oxidatively modified1

Superoxide dismutase1

Degradation3

Enzymes1

Induce1

Vitro2

Lipoprotein4

Tissues2

Aldehydes2

Hydroperoxides3

Biomolecules1

Products6

Extracellular2

Marker1

Assay1

Malondialdehyde2

Mitochondrial protein2

Cytoplasmic2

Hepatic2

Amino groups1

Cardiovascular1

Oxidative modifications1

Inactivate1

Occurs1

Amyloid3

Rats1

Damage3

Genes2

Human6

Serum2

Apoptosis3

Levels3

Carbonyl3

Antibodies2

Cellular1

Reaction1

Cytochrome1

Cell1

Glucose1

Results2

Inflammation1

Stress2

Blood2

Molecular4

Mechanisms1

Soluble1