AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsInformation Science
Cyclin D1Proliferating Cell Nuclear AntigenCell CycleCyclin ACyclin ECyclin BCyclin B1Cyclin D2Cyclin D3Cyclin A1Cyclin A2Cyclin DCyclin G1Cyclin GCyclinsCyclin CCyclin-Dependent KinasesCyclin B2Cyclin TCyclin-Dependent Kinase 2Cyclin G2Cyclin HCyclin-Dependent Kinase 4CDC2-CDC28 KinasesCDC2 Protein KinaseG1 PhaseCell Cycle ProteinsCyclin-Dependent Kinase Inhibitor p27S PhaseRetinoblastoma ProteinCyclin IMitosisOncogene ProteinsGenes, bcl-1Protein-Serine-Threonine KinasesCyclin-Dependent Kinase 6PhosphorylationCyclin-Dependent Kinase Inhibitor p21Cell DivisionG2 PhaseCell ProliferationTumor Suppressor ProteinsCell Line, TumorRNA, MessengerE2F Transcription Factorscdc25 PhosphatasesMolecular Sequence DataProto-Oncogene ProteinsCell NucleusTumor Cells, CulturedMicrotubule-Associated ProteinsGene Expression Regulation, NeoplasticF-Box ProteinsProtamine KinaseG0 PhaseCell LineProtein BindingBlotting, WesternMaturation-Promoting FactorDown-RegulationImmunohistochemistryTranscription FactorsTransfectionPromoter Regions, GeneticCyclin-Dependent Kinase Inhibitor p16Nuclear ProteinsGene Expression RegulationTranscription Factor DP1ApoptosisCyclin-Dependent Kinase 9HeLa CellsStarfishSignal Transduction3T3 CellsRetinoblastoma-Binding Protein 1Tumor Suppressor Protein p53Transcription, GeneticE2F1 Transcription FactorDNA-Binding ProteinsRetinoblastoma-Like Protein p107Amino Acid SequenceBase Sequence
KinasesCDKsLevels of cyclinPhosphorylationGeneSubstratesInhibitorsSpecificityDependentProgressionDifferent cyclinsAntibodiesRetinoblastomaInhibitionMitoticComplexesEukaryotesRegulatoryCcnb1ip1GenesCdk1CytoplasmPosttranslational modificationsReplicationComplementSubtypeMutationExpressionMammaryBindCellular proteinsNuclearPhosphorylatesCDK2EnzymesTumor suppressorInteractionNeurotransmissionApoptoticMutationsAntibodyHuman
Kinases18
  • In the cyclin-dependent kinase (CDK) family or CDK, Cyclin, and CKIs, serine/threonine kinases play an integral role in regulating the eukaryotic cell cycle. (wikipedia.org)
  • Cyclin-dependent kinases (Cdks) are principal drivers of cell division and are an important therapeutic target to inhibit aberrant proliferation. (escholarship.org)
  • Cell-cycle events are controlled by cyclin-dependent kinases (CDKs), whose periodic activation is driven by cyclins. (nature.com)
  • Brown, N. R., Noble, M. E., Endicott, J. A. & Johnson, L. N. The structural basis for specificity of substrate and recruitment peptides for cyclin-dependent kinases. (nature.com)
  • This gene encodes a protein involved in regulation of the cell cycle through interactions with several cyclin-dependent kinases. (nih.gov)
  • Cyclins function as regulators of CDK kinases. (novusbio.com)
  • Protein kinases are one of the largest and most influential of gene families: constituting some 2% of the proteome, they regulate almost all biochemical pathways and may phosphorylate up to 30% of the proteome. (wormbook.org)
  • Protein kinases constitute one of the largest and most important of protein families, accounting for ~2% of genes in a variety of eukaryotic genomes. (wormbook.org)
  • By phosphorylating substrate proteins, kinases modify the activity, location and affinities of up to 30% of all cellular proteins, and direct most cellular processes, particularly in signal transduction and co-ordination of complex pathways. (wormbook.org)
  • Most protein kinases share a common ePK (eukaryotic protein kinase) catalytic domain, and can be identified by sequence similarity with Blast or profile hidden Markov models (HMMs). (wormbook.org)
  • The remaining atypical protein kinases (aPK) belong to several families, some of which have structural, but not sequence similarity to ePKs. (wormbook.org)
  • We identified 438 protein kinase genes, including 20 atypical kinases, and an additional 25 kinase fragments or pseudogenes. (wormbook.org)
  • Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. (bdbiosciences.com)
  • Cyclins and cyclin dependent kinases, or CDK. (jove.com)
  • Positive regulators include two protein groups that allow cells to pass through regulatory checkpoints: cyclins and cyclin-dependent kinases (CDKs). (jove.com)
  • Among the major cyclin-dependent kinases (Cdks), Cdk1 is most abundantly expressed in IDG-SW3 cells, and its expression is down-regulated during differentiation into osteocytes. (lu.se)
  • Protein kinases that control cell cycle progression in all eukaryotes and require physical association with CYCLINS to achieve full enzymatic activity. (bvsalud.org)
  • Cyclin-dependent kinases are regulated by phosphorylation and dephosphorylation events. (bvsalud.org)
CDKs7
  • Cyclin-dependent kinase inhibitor proteins work by inactivating the CDKs by degradation. (wikipedia.org)
  • Human cells contain many different cyclins binding to different CDKs. (wikipedia.org)
  • CDKs and cyclins appear and activate at specific cell cycle phases. (wikipedia.org)
  • Further research has demonstrates that Cdks, cyclins and CKIs play essential roles in processes such as transcription, epigenetic regulation, metabolism, stem cell self-renewal, neuronal functions and spermatogenesis. (wikipedia.org)
  • Cyclins (regulatory subunits) bind to cdks (catalytic subunits) to form complexes that regulate the progression of the cell cycle. (bdbiosciences.com)
  • The main cyclin-cdks complexes formed in vertebrate cells are cyclin D-cdk4 (G0/G1), cyclin E-cdk2 (G1/S), cyclin A-cdk2 (S) and cyclin B1-cdk1 (G2/M). These complexes are regulated by activating and inhibitory phosphorylation events, as well as by interactions with small regulatory proteins including p21 and p27Kip1. (bdbiosciences.com)
  • Thus, levels of the four different cyclins vary in predictable patterns and combine with consistent CDKs at specific points to achieve forward momentum. (jove.com)
Levels of cyclin3
  • The only exception, in the latter group, was patients with tumours expressing high levels of cyclin D1, who did as well as the high p27 group. (ox.ac.uk)
  • The more aggressive basal-like subtype of breast cancers, however, exhibited lower levels of cyclin D1 and Dicer, which would in turn globally reduce the level of mature miRNA. (sciencedaily.com)
  • Exposure of cells to cadmium , as well as the specific silencing of eIF4E gene, also resulted in decreased cellular levels of cyclin D1, a critical cell cycle and growth regulating gene, suggesting that the observed inhibition of cyclin D1 gene expression in the cadmium -treated cells is most likely due to decreased cellular level of eIF4E. (cdc.gov)
Phosphorylation6
  • another is mutation of T286 in cyclin D1 (CCND1) causing the loss of phosphorylation of T286 is involved in nuclear accumulation of cyclin D1 in esophageal cancer [ 14 ]. (hindawi.com)
  • Furthermore, p100 suppressed the expression of PHLPP2 (PH domain and leucine-rich repeat protein phosphatases 2), thus promoting CREB phosphorylation at Ser133 and subsequently leading to miR-302d transcription. (oncotarget.com)
  • Here we compare the specificity of two budding yeast cyclins, the S-phase cyclin Clb5 and the M-phase cyclin Clb2, in the phosphorylation of 150 Cdk1 (Cdc28) substrates. (nature.com)
  • Here DARPP-32 phosphorylation by protein kinase A (PKA), DARPP-32 into a powerful protein phosphatase 1 (PP1) inhibitor. (pp1a.com)
  • DARPP-32 can itself inhibit the following PKA DARPP-32 phosphorylation by cyclin-dependent kinase 5 (Cdk5). (pp1a.com)
  • Magnesium is involved in nearly every aspect of biochemical metabolism (eg, DNA and protein synthesis, glycolysis, oxidative phosphorylation). (medscape.com)
Gene13
  • methylation at certain residues of histones can regulate gene expression [ 4 ], and glycosylation is responsible for targeting substrates and changing protein half-life [ 2 ]. (hindawi.com)
  • Either at genomic or at proteomic level, mutations have significant impact on normal gene or protein function, and human diseases could be associated with mutations like nonsynonymous single-nucleotide variations (nsSNVs) on amino acids. (hindawi.com)
  • Yet how gene mutations affect protein activities through posttranslational modification sites have not been widely studied. (hindawi.com)
  • There was a statistically significant association between the expression of p27 and both cyclin D1 and the retinoblastoma gene product (pRb), corresponding to their close interactions in regulating the G1/S transition in the cell cycle. (ox.ac.uk)
  • This study found an increase in excess GR transcriptional activity of protein phosphatase 1 alpha (PP1α) in HEK-293 cells and a decrease in the expression levels of GR-responsive gene knockdown following PP1α model A549 cells endogenously . (pp1a.com)
  • This gene is located in a cluster of closely related salivary proline-rich proteins on chromosome 12. (cancerindex.org)
  • What does this gene/protein do? (cancerindex.org)
  • One study (PMID: 16177568) reported aberrant splicing of transcripts from this gene which results in removal of the cyclin binding domain only in human cancer cells, and reduction in gene expression was shown in colorectal cancers (PMID: 17982127).Multiple transcript variants encoding different isoforms have been found for this gene. (nih.gov)
  • The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. (novusbio.com)
  • Dr. Pestell and colleagues developed transgenic mice that could induce cyclin D1 expression in the breast and examined cells with cyclin D1 gene deleted. (sciencedaily.com)
  • Because the cyclin D1 gene has been implicated in a variety of other human cancers these findings may have broad implications for processing of non coding RNA in human tumorigenesis. (sciencedaily.com)
  • Protein and gene expression of apoptotic signaling pathway-related proteins such as caspase-3, cytochrome c, and PARP were determined to provide evidence for the mechanisms of action of Tan IIA in the treatment of psoriasis. (hindawi.com)
  • The molecular consequence of translocation is overexpression of the protein cyclin D1 (coded by the PRAD1 gene located close to the breakpoint). (medscape.com)
Substrates3
  • Spy1 lacks the cyclin-binding site that mediates p27 and substrate affinity, explaining why Cdk-Spy1 is poorly inhibited by p27 and lacks specificity for substrates with cyclin-docking sites. (escholarship.org)
  • Identification of a cyclin-cdk2 recognition motif present in substrates and p21-like cyclin-dependent kinase inhibitors. (nature.com)
  • Specific substrates for cdk-cyclin complexes include nuclear lamins, histones, oncogenes (e.g., c-abl and SV40 large T-Ag), tumor suppressor genes (e.g., retinoblastoma protein, Rb), nucleolin and others. (bdbiosciences.com)
Inhibitors4
  • Role in cancer: Cyclin-dependent kinase inhibitors (CKIs) mutants are frequent in human cancers. (wikipedia.org)
  • Cdk enzymatic activity is tightly controlled through cyclin interactions, posttranslational modifications, and binding of inhibitors such as the p27 tumor suppressor protein. (escholarship.org)
  • Synthesis and evaluation of pyrazolo[1,5-b]pyridazines as selective cyclin dependent kinase inhibitors. (expasy.org)
  • In addition, exposure of cells to cadmium resulted in enhanced ubiquitination of eIF4E protein while inhibitors of proteasome activity reversed the cadmium -induced decrease of eIF4E protein. (cdc.gov)
Specificity3
Dependent22
  • A cyclin-dependent kinase inhibitor protein (also known as CKIs, CDIs, or CDKIs) is a protein which inhibits the enzyme cyclin-dependent kinase (CDK) and Cyclin activity by stopping the cell cycle if there are unfavorable conditions, therefore, acting as tumor suppressors. (wikipedia.org)
  • Cell cycle progression is stopped by Cyclin-dependent kinase inhibitor protein at the G1 phase. (wikipedia.org)
  • Cyclin-dependent kinase inhibitor proteins are essential in the regulation of the cell cycle. (wikipedia.org)
  • Cyclin-dependent kinase inhibitor proteins use ATP as a phosphate contributor to phosphorylate serine and threonine residues. (wikipedia.org)
  • Seven cyclin-dependent kinase inhibitor proteins have been identified. (wikipedia.org)
  • These cyclin-dependent kinase inhibitor protein emerges only in their specific cell cycle phase. (wikipedia.org)
  • The discovery of Cyclin-dependent kinase inhibitor proteins in 1990 opened the door in how we think about cell cycle control. (wikipedia.org)
  • In mammals, p27, a cyclin-dependent kinase inhibitor protein, helps control CDK activity in G1. (wikipedia.org)
  • It moves into the cytoplasm and eventually activates a specific cyclin-dependent kinase (CDK). (wikipedia.org)
  • Cyclin-dependent kinase 5 and its activator p35 disrupt Munc18a-syntaxin 1 binding, thereby promoting synaptic vesicle fusion during exocytosis. (eur.nl)
  • We investigated protein levels of the signaling pathway: p35, cyclin-dependent kinase 5, Munc18a, syntaxin 1A and 1B, Munc18-interacting protein 1 and Munc18-interacting protein 2 in Alzheimer's disease cortex and found that this pathway was up-regulated in the Alzheimer's disease parietal and occipital cortex. (eur.nl)
  • Inhibitor-1, the first identified endogenous inhibitor of protein phosphatase 1 (PP-1), was previously reported to be a substrate for cyclin-dependent kinase 5 (Cdk5) at Ser 67 . (elsevierpure.com)
  • Dephosphorylation of inhibitor-1 at Thr 35 is equivalent to inactivation of the protein, as inhibitor-1 only serves as an inhibitor of PP-1 when phosphorylated by cAMP-dependent kinase (PKA) at Thr 35 . (elsevierpure.com)
  • Targets of the cyclin-dependent kinase Cdk1. (nature.com)
  • Schulman, B. A., Lindstrom, D. L. & Harlow, E. Substrate recruitment to cyclin-dependent kinase 2 by a multipurpose docking site on cyclin A. (nature.com)
  • Previously, we found that eukaryotic initiation factor (eIF) 4A interacts with cyclin dependent kinase A (CDKA), the plant ortholog of mammalian CDK1. (aber.ac.uk)
  • Cyclin-dependent kinase inhibitor p27Kip1 expression and interaction with other cell cycle-associated proteins in mammary carcinoma. (ox.ac.uk)
  • Blebbing, apoptotic body formation and protein release during early apoptosis are dependent on ROCK and myosin ATPase activity to drive actomyosin contraction. (nature.com)
  • We identified 231 proteins released from actomyosin contraction-dependent blebs and apoptotic bodies by adapted SILAC (stable isotope labeling with amino acids in cell culture) combined with mass spectrometry analysis. (nature.com)
  • Dysregulation of CDK8 (Cyclin-Dependent Kinase 8) and its regulatory partner CycC (Cyclin C) , two subunits of the conserved Mediator (MED) complex, have been linked to diverse human diseases such as cancer. (sdbonline.org)
  • Tan IIA inhibited proliferation of mouse keratinocytes in a dose- and time-dependent manner and induced apoptosis, resulting in S phase arrest accompanied by down-regulation of pCdk2 and cyclin A protein expression. (hindawi.com)
  • Cdk5 is named after its structural similarity to members of the serine/threonine cyclin-dependent kinase family. (frontiersin.org)
Progression3
  • We show that the effects on cell cycle progression stemmed from reduced ribosome content and translational capacity, which suppressed the accumulation of cyclins at the translational level. (nih.gov)
  • Knockdown of p100 in cultured human bladder cancer cells promoted anchorage-independent growth accompanied with elevating abundance of cell-cycle-related proteins and accelerated cell-cycle progression. (oncotarget.com)
  • The work supports the idea that cancer-causing proteins like cyclin D1 may drive cancer progression in part via miRNA biogenesis. (sciencedaily.com)
Different cyclins1
  • Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. (novusbio.com)
Antibodies2
  • Double staining of primate cells with anti-cyclin B and anti-MAP4 antibodies demonstrated these two antigens were colocalized on microtubules and copartitioned following two treatments that altered MAP4 distribution. (rupress.org)
  • Abcam: antibodies, proteins, kits. (abcam.com)
Retinoblastoma3
Inhibition2
  • Humans have evolved elaborate mechanisms to activate p53 in response to insults that lead to cancer, including the binding and inhibition of Hdm2 by the 60S ribosomal proteins (RPs) RPL5 and RPL11. (nih.gov)
  • The effects of POP inhibition and knockdown on the proliferation of cultured human estrogen receptor-positive (ER+) MCF7 and T47D, and ER-negative (ER-) MDA-MB-231 breast cancer cell lines and the MCF12A non-tumorigenic epithelial cell line were tested by analyzing their influence on cell proliferation (WST-1 assay), cell viability (trypan blue exclusion assay), and cell cycle arrest (cell cycle analysis, cell cycle regulator proteins expression). (cancerindex.org)
Mitotic3
Complexes2
  • Here we show that this interaction occurs only in proliferating cells where the two proteins co-associate with 5'-cap-binding protein complexes, eIF4F or the plant specific eIFiso4F. (aber.ac.uk)
  • This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. (novusbio.com)
Eukaryotes1
  • These proteins are present in eukaryotes, ranging from yeast to humans. (jove.com)
Regulatory3
  • Spy1/RINGO (Spy1) proteins bind and activate Cdk but are resistant to canonical regulatory mechanisms that establish cell-cycle checkpoints. (escholarship.org)
  • PTMs are involved in many protein activities and cellular processes, such as protein folding, stability, conformation, and some significant regulatory mechanisms [ 1 ]. (hindawi.com)
  • Further, we reveal that cell routine protein and apoptotic markers, aswell as essential NF-B regulatory substances, are modulated by treatment with LOE, thus shedding light on the mechanism of actions behind the anticancer ramifications of LOE. (unambitiousus.com)
Ccnb1ip11
  • Full length human recombinant protein of human CCNB1IP1 (NP_878269) produced in E.coli. (origene.com)
Genes2
  • It promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. It also acts as a transcription repressor of E2F1 target genes. (nih.gov)
  • Taken together, our results demonstrate that the exposure of cells to cadmium chloride resulted in cytotoxicity and cell death due to enhanced ubiquitination and consequent proteolysis of eIF4E protein, which in turn diminished cellular levels of critical genes such as cyclin D1. (cdc.gov)
Cdk12
  • About 24% of these proteins were phosphorylated more efficiently by Clb5-Cdk1 than Clb2-Cdk1. (nature.com)
  • Cyclin A is involved in both S-phase and G2/M transitions of the cell cycle through its association with cdk2 and cdk1, respectively. (bdbiosciences.com)
Cytoplasm1
  • A cells cycle is positively regulated promoting progress through the stages via the interaction of two classes of proteins found in the cytoplasm. (jove.com)
Posttranslational modifications2
  • Protein posttranslational modifications (PTMs) play key roles in a variety of protein activities and cellular processes. (hindawi.com)
  • More than 200 different types of protein posttranslational modifications (PTMs) have been detected. (hindawi.com)
Replication3
  • MCM2 (Mini chromosome maintenance protein 2) is involved in regulating DNA replication. (thermofisher.com)
  • MCM2 (also called CDCL1, mitotin and BM28), is a human nuclear protein that is crucial in the cell cycle, being involved in the onset of DNA replication and cell division. (thermofisher.com)
  • For instance, during G1, when one type of cyclin, named D, is synthesized and binds to a CDK, the cell transitions into S phase, as another cyclin, E, peaks and forms a complex with CDK to promote DNA replication. (jove.com)
Complement1
  • In vivo, a phospho-null (APGR) variant of the Arabidopsis eIF4A1 protein retains the ability to functionally complement a mutant (eif4a1) plant line lacking eIF4A1, whereas a phosphomimetic (EPGR) variant fails to complement. (aber.ac.uk)
Subtype1
  • They found that patients with the luminal A subtype of breast cancer had increased levels of expression of both cyclin D1 and Dicer. (sciencedaily.com)
Mutation1
  • In the cortex of transgenic Tg2576 mice over-expressing human β-amyloid precursor protein with the Swedish mutation known to lead to familial Alzheimer's disease, which have substantial levels of β-amyloid peptide but lack neurofibrillary tangles and neuron loss, no alterations of protein levels were detected. (eur.nl)
Expression8
  • Here, we are, for the first time, to report that p100 protein expression was dramatically decreased in bladder cancers of N-butyl-N-(4-hydroxybutyl)-nitrosamine (BBN)-treated mice and human patients. (oncotarget.com)
  • When the combined expression of p27 and cyclin D1 was related to survival, patients with high levels of p27, regardless of their cyclin D1 status, did well, whilst those with low p27 had a poor outcome. (ox.ac.uk)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • Western Blot: Cyclin H Antibody (1B8) [H00000902-M01] - CCNH monoclonal antibody (M01), clone 1B8 Analysis of CCNH expression in HeLa. (novusbio.com)
  • In prior work, they showed that cyclin D1 regulates the non coding genome, and that the non-coding genome, in turn, regulates expression of cyclin D1. (sciencedaily.com)
  • Epstein-Barr virus latent membrane protein expression and cyclin DI cell cyase protein expression in malignant and normal oesophageal tissues to see whether any variation in their expression in these tissues could be of diagnostic or prognostic value. (bvsalud.org)
  • EBV-LMPI protein expression and cyclin DI expression were studied immunohisto chemically in these tissue sections. (bvsalud.org)
  • 2 cases of EBV-LMPI protein expression did not express p53 protein and 7 cases did not express both p53 and EBV-LMPI proteins. (bvsalud.org)
Mammary1
  • Using antisense RNA, Dr. Pestell's group was the first to show that cyclin D1 drives mammary tumor growth in vivo. (sciencedaily.com)
Bind2
  • Mechanistically, p100 inhibited Cyclin D1 protein translation by activating the transcription of LARP7 and its hosted miR-302d, which could directly bind to 3′-UTR of cyclin d1 mRNA and inhibited its protein translation. (oncotarget.com)
  • They must bind to a specific cyclin to be activated. (jove.com)
Cellular proteins1
  • The HPV E7 peptide contains the LxCxE motif, shared by other Rb-binding viral and cellular proteins. (nih.gov)
Nuclear1
  • Dying cells have been defined as apoptotic by distinguishing features, including cell contraction, nuclear fragmentation, blebbing, apoptotic body formation and maintenance of intact cellular membranes to prevent massive protein release and consequent inflammation. (nature.com)
Phosphorylates1
  • The active cyclin/CDK complex then phosphorylates proteins, activates them, and sends the cell into the next phase of the cell cycle. (wikipedia.org)
CDK21
  • The structure of CDK2-CyclinA and p27 is determined by crystallography, demonstrating that the inhibitor of p27 stretches at the top of the Cyclin-CDK complex. (wikipedia.org)
Enzymes1
  • E is then degraded by cytoplasmic enzymes and cyclin A concentrations increase throughout the S phase and remain high into G2 to promote entry into the M phase when in an active complex. (jove.com)
Tumor suppressor2
  • Although the precursor protein of NFκB2 (p100) is thought to act as a tumor suppressor in mammalian cells, the molecular mechanism of its anti-tumor activity is far from clear. (oncotarget.com)
  • Although the diverse tumor-promoting roles of NFκB in cancer cell proliferation, anti-apoptosis, angiogenesis, invasion and metastasis, are well established [ 3 - 9 ], much less is known about how p100, a precursor protein of NFκB2, acts as a tumor suppressor in many mammalian cells [ 10 ]. (oncotarget.com)
Interaction2
  • Cyclin B interaction with microtubule-associated protein 4 (MAP4) targets p34cdc2 kinase to microtubules and is a potential regulator of M-phase microtubule dynamics. (rupress.org)
  • Faha B, Ewen ME, Tsai LH, Livingston DM, Harlow E. Interaction between human cyclin A and adenovirus E1A-associated p107 protein. (bdbiosciences.com)
Neurotransmission1
  • Dopamine and cyclic-AMP activated Mr32kDa phosphoprotein (DARPP-32) is a central signaling proteins in neurotransmission. (pp1a.com)
Apoptotic3
  • We now show that during early apoptosis limited membrane permeabilization occurs in blebs and apoptotic bodies, which allows release of proteins that may affect the proximal microenvironment before the catastrophic loss of membrane integrity during secondary necrosis. (nature.com)
  • These results indicate that limited membrane permeabilization occurs in blebs and apoptotic bodies before secondary necrosis, leading to acute and localized release of immunomodulatory proteins during the early phase of active apoptotic membrane blebbing. (nature.com)
  • 2 , 3 A key feature of apoptosis has been defined as the maintenance of an intact cellular membrane (detectable as exclusion of impermeable dyes such as propidium iodide, PI) throughout the apoptotic program to prevent intracellular protein release and subsequent immunological activation. (nature.com)
Mutations1
Antibody7
  • Immunocytochemistry/ Immunofluorescence: Cyclin H Antibody (1B8) [H00000902-M01] - Analysis of monoclonal antibody to CCNH on HeLa cell. (novusbio.com)
  • Immunohistochemistry-Paraffin: Cyclin H Antibody (1B8) [H00000902-M01] - Analysis of monoclonal antibody to CCNH on formalin-fixed paraffin-embedded human testis. (novusbio.com)
  • Sandwich ELISA: Cyclin H Antibody (1B8) [H00000902-M01] - Detection limit for recombinant GST tagged CCNH is approximately 1ng/ml as a capture antibody. (novusbio.com)
  • Quality control test: Antibody Reactive Against Recombinant Protein. (novusbio.com)
  • Antibody reactivity against cell lysate and recombinant protein for WB. (novusbio.com)
  • ATCC CCL-243) were probed with the mouse anti-human cyclin A antibody at concentration of 2.0 µg/mL (lane 1), 1.0 µg/mL (lane 2), and 0.5 µg/mL (lane 3). (bdbiosciences.com)
  • Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. (bdbiosciences.com)
Human3
  • The research suggests that a protein strongly implicated in human cancer also governs the non-protein-coding genome. (sciencedaily.com)
  • Human cyclin A has been reported to migrate between 54-60 kDa by SDS-PAGE and clone BF683 reportedly does not cross-react with mouse, rat or mink cyclin A. (bdbiosciences.com)
  • Exposure of human cell lines, HCT15, PLC/PR/5, HeLa, and Chang, to cadmium chloride resulted in cytotoxicity and cell death, and this was associated with a significant decrease in eIF4E protein levels. (cdc.gov)