Proteins binding competitive. Medical search. Frequent questions

The Access Total T3 assay is a competitive binding immunoenzymatic assay. (cdc.gov)
C. rodentium ΔafuA was significantly impaired in an in vivo murine competitive assay as well as its ability to transmit infection from an afflicted to a naïve murine host. (rcsb.org)
In a subsequent angiotensin-converting enzyme 2 (ACE2)-spike surrogate neutralization assay, P5C3, and P2G3 exhibited broad and highly potent inhibition of binding (of VOC spike proteins) to ACE2. (news-medical.net)
97 Pages Report] The protein binding assay market is projected to reach USD 425.7 million in 2023 from USD 231.3 million in 2017, at CAGR of 10.7% during the forecast period. (marketsandmarkets.com)
Top-down and bottom-up approaches were used to validate the size of the global protein binding assay market and estimate the size of various other dependent submarkets. (marketsandmarkets.com)
The key players operating in the protein binding assay market are Thermo Fisher Scientific (US), GE Healthcare (US), Danaher (US), and Merck (Germany). (marketsandmarkets.com)
Fluorescence microscopy, fluorescent retinoid binding assay, fluorescence spectroscopy. (sigmaaldrich.com)
LightOx23 can be utilised to study retinoid behaviour using fluorescence microscopy, employed in a competitive fluorometric ligand-binding assay for direct determination of the strength of binding of non-fluorescent retinoids to a protein target, and as a fluorophore in fluorescence spectroscopy. (sigmaaldrich.com)
To provide further insight into these glycan attachments and their potential clinical relevance, the classic hemagglutination (HA) assay was applied using spike protein from the Wuhan, Alpha, Delta and Omicron B.1.1.529 lineages of SARS-CoV-2 mixed with human RBCs. (biorxiv.org)
A pull-down assay using delphinidin-Sepharose beads revealed that delphinidin binds directly with MAPKK4 or PI-3K in a manner that was competitive with adenosine triphosphate. (oregonstate.edu)
Silver enhancement of the bound nanoparticles may be used to increase the sensitivity of the assay. (hindawi.com)
b) Schematic drawing of a bioanalytical REA assay before (left) and after (right) binding of AuNP-analyte complexes to the capture protein layer. (hindawi.com)
25-OHD (25-hydroxyvitamin D), the major circulating metabolite of vitamin D, was measured in serum by competitive protein-binding (CPB) assay and radioimmunoassay kit (Nichols, San Juan Capistrano, California). (who.int)
In the early 1970s, Belsey et al and Haddad et al developed a competitive binding assay to measure serum vitamin D levels. (medscape.com)
This assay used vitamin D-binding protein (DBP) obtained from rat serum as the binding agent and exploited the capacity of vitamin D in test samples to displace bound [ 3 H]25(OH)D 3 from DBP. (medscape.com)

These mechanisms include, competitive inhibition or negative feedback. (wikipedia.org)
After the secretion, 5-HT increases the effects of prothrombotic agents by its binding with 5-HT2 receptors Thromboregulation is also in charge of regulating the process of clot elimination, called primary hemostasis inhibition. (wikipedia.org)
PGE1 inhibits the secretion of factors that stimulate platelet aggregation by competitive inhibition. (wikipedia.org)
Cilastatin prevents renal metabolism of imipenem by competitive inhibition of dehydropeptidase along the brush border of the renal tubules. (medscape.com)
Selective activation or inhibition of a particular protein function can help elucidate crucial molecular mechanisms and enables important advances in cell biology. (ku.edu)
Small-molecule controlled molecular systems also possess tremendous value in bioengineering and biomedical applications: activation of protein function allows the construction of protein switches and biosensor proteins, whereas inhibition of protein function contributes to the development of novel therapeutic agents. (ku.edu)
To this end I explored two parallel paths, one to study activation and one to study inhibition of protein functions. (ku.edu)
In parallel to these efforts I carried out studies aimed at inhibition of protein function, as exemplified by my project that uses small molecules to disrupt a protein-RNA interaction. (ku.edu)
Inhibition of spike protein-induced HA was tested using the macrocyclic lactone ivermectin (IVM), which is indicated to bind strongly to SARS-CoV-2 spike protein glycan sites. (biorxiv.org)

Kistrin is a protein inhibitor of platelet aggregation. (wikipedia.org)
The most effective inhibitor was harmaline (Ki = 94 +/- 21 micromol/l), a known competitive inhibitor of Na+ binding to certain transport proteins. (erowid.org)
Conventional methods of inhibitor design mostly target RNA-processing enzymes and cannot be generalized to the majority of RNA-binding proteins (RBPs). (ku.edu)
[ 135 ] Total hormone assays necessitate the inclusion of an inhibitor (displacing or blocking agent) such as 8-anilino-l-napthalene-sulphonic acid (ANS), or salicylate to release the hormone from binding proteins. (medscape.com)
This can result in the protein binding inhibitor reagent (e.g. (medscape.com)
In addition, this tripeptide was shown to be a competitive inhibitor of apigenin-stimulated GSH transport ( K i value of 14 μM), confirming that it either interacts with the same site on MRP1 as GSH or that the binding of the two tripeptides is mutually exclusive. (aspetjournals.org)

In this study, the binding affinity and mechanism of human odor-binding protein OBP2a to 14 typical odorants in water were first assessed using fluorescent competitive binding assays and molecular docking techniques. (springeropen.com)
A solid background in cell biology, protein activity assays and NGS analyses would be advantageous, but not mandatory. (eurosciencejobs.com)
However, the determination of RBP binding sites on a large scale is a challenging task due to high cost of biochemical assays. (biomedcentral.com)
[ 136 ] The displacement of hormone binding from serum proteins by such agents, together with the large sample dilution employed in modern assays, facilitates the binding of hormone to the antibody reagent. (medscape.com)
The compound is a weak ligand for the retinoic acid receptor proteins (RARs) and related cellular retinoic acid binding proteins (CRABPI/II) and can, therefore, be used as a replacement for ATRA in cellular assays involving retinoid signaling processes. (sigmaaldrich.com)
Fluorescent retinoic acid analogues for use in cellular imaging, protein-ligand binding assays, and flow cytometry to bypass traditional problems associated with fluorescent probes. (sigmaaldrich.com)
[ 10 ] In general, these assays depend on an antibody that recognizes 25(OH)D2 and 25(OH)D3 in equimolar proportions and the competitive displacement of tracer 25(OH)D (25(OH)D that has been tagged with a radioactive isotope such as 125-I or a chemiluminescent ligand) by acetonitrile extracted samples. (medscape.com)

Of the resulting antigen/antibody complexes, the T3 analogue/antibody complexes are bound to the streptavidin coated solid phase. (cdc.gov)
Separation in a magnetic field and washing removes the sample T3/antibody complexes and other materials not bound to the solid phase. (cdc.gov)
The crystal structures of the two apo-proteins and their complexes with the Strep-tag II peptide were refined at resolutions below 2 A. Both in the presence and absence of the peptide, the lid-like loop next to the ligand pocket--comprising residues 45 through 52--adopts an 'open' conformation in all four subunits within the asymmetric unit. (rcsb.org)
In one study of a large set of protein-ligand complexes, we tried to improve the free energies of binding by using MD simulations with QM-derived charges, which sometimes led to improved results, but not always. (lu.se)

I contributed to the development of a general strategy of designing competitive inhibitors targeting RBPs. (ku.edu)
Therefore, our key goals are to define how and why these novel secreted RBPs bind RNA in human milk and the gastrointestinal tract. (eurosciencejobs.com)
RNA binding proteins (RBPs) play a vital role in post-transcriptional processes in all eukaryotes, such as splicing regulation, mRNA transport, and modulation of mRNA translation and decay. (biomedcentral.com)

TX2 effects are mediated by G protein-coupled receptors, subtypes TPα and TPβ. (wikipedia.org)
PGI2, prostacyclin, binds to IP receptors that catalyze cAMP formation. (wikipedia.org)
PGE1 binds to IP receptors. (wikipedia.org)
IP receptors bind with ionophores that induce ADP and serotonin secretion. (wikipedia.org)
Because the neurobiological substrate of cariprazine has remained elusive, we took advantage of PharmacoSTORM to provide in vivo evidence that cariprazine predominantly binds to D 3 dopamine receptors on Islands of Calleja granule cell axons but avoids dopaminergic terminals. (nature.com)
LTs exert their biological effects by activating specific receptors belonging to the superfamily of G protein-coupled receptors (GPCRs) [ 22 - 24 ]. (hindawi.com)
I also apply a tool, recently developed by myself and discussed in Chapter 3, to distinguish capsids that bind to different receptors. (caltech.edu)
In drug discovery, it is of utmost importance to accurately calculate the free energies of binding ligands to various protein targets, such as enzymes and receptors. (lu.se)

The binding affinity of OBP2a to fatty aldehydes including hexanal, heptanal, 2-octenal, decanal, and β-cyclocitral increased with the increase of hydrophobicity of aldehydes. (springeropen.com)
Affinity chromatography resins, including protein A based resin are considered to be the most expensive as compared to others. (prnewswire.co.uk)
The Strep-tag II is a nine-amino acid peptide that was developed as an affinity tool for the purification of corresponding fusion proteins on streptavidin columns. (rcsb.org)
BLT2 is a pharmacologically distinct receptor, which is ubiquitously expressed and displays low affinity for LTB4 and also binds other eicosanoids [ 26 , 27 ]. (hindawi.com)
P2G3 showed the highest binding affinity for the spike protein from ancestral SARS-CoV-2 and a panel of Alpha, Beta, Gamma, and Delta spike proteins . (news-medical.net)

Cell-free genomics reveal intrinsic, cooperative and competitive interactions of chromatin binding proteins. (uni-muenchen.de)
By focusing on the stabilizing protein tropomyosin Cdc8, bundling protein fimbrin Fim1, and severing protein coffin Adf1, we examined how their pairwise and collective interactions with actin filaments regulate their activity and segregation to functionally diverse F-actin networks. (elifesciences.org)
Our findings reveal that competitive and cooperative interactions between actin binding proteins help define their associations with different F-actin networks. (elifesciences.org)
As is shown in Table 1 , these methods mostly employ RNA sequence and structural information to predict protein-RNA interactions. (biomedcentral.com)
Oli [ 16 ] uses k-mer frequency as input feature into an SVM classifier to predict RNA-protein interactions. (biomedcentral.com)
We mostly used molecular mechanics (MM) in order to model the protein-ligand interactions, which is more approximate than quantum-mechanical (QM) methods, but necessary to reduce the computational cost when doing calculations on protein-ligand systems, which often contain tens of thousand of atoms. (lu.se)

Serum or plasma vitamin B12 has been measured using commercially available competitive protein binding immunoassays (CPBA). (kcl.ac.uk)
Currently, serum TT4 and TT3 concentrations are measured by competitive immunoassay methods that are now mostly non-isotopic and use enzymes, fluorescence or chemiluminescence molecules as signals. (medscape.com)
[ 140 ] Secondly, the diluents used to reconstitute L-T4 and L-T3 preparations for use as calibrators are either modified protein matrices or human serum pools that have been stripped of hormone by various means. (medscape.com)
Ensuring that during the test process, the amount of thyroid hormone released from serum binding proteins is the same as that released in the presence of the calibrator diluent. (medscape.com)

Mattiasson B. Continuous measurements of a binding reaction using a capacitive biosensor. (lu.se)
Labib, M., Hedström, M. , Amin, M., Mattiasson, B. A novel competitive capacitive glucose biosensor based on concanavalin A-labeled nano-gold colloids assembled on a polytyramine-modified gold electrode. (lu.se)

The peptide recognizes the same pocket of streptavidin where the natural ligand is normally bound so that biotin or its chemical derivatives can be used for competitive elution. (rcsb.org)
Both streptavidin mutants carry mutations at positions 44, 45, and 47, that is, in a flexible loop region close to the binding site. (rcsb.org)
Upon addition of a sample containing the analyte of interest, a NP-protein-analyte complex is formed in the test device that is captured on the REA chip, for example, via streptavidin-biotin interaction. (hindawi.com)
Herein, we demonstrate that adsorptive immobilization via a cationic polymeric interlayer is a competitive and fast technique for the binding of the capture protein streptavidin onto planar SiO 2 surfaces such as REA biochips. (hindawi.com)

If cyanate is produced in vivo from urea in patients with uremia, plasma protein carbamylation may play a role in the decreased plasma protein binding of some acidic drugs. (nih.gov)

Clarithromycin inhibits bacterial growth by binding to the 50S ribosomal subunit and inhibiting protein synthesis. (medscape.com)
Tobramycin inhibits bacterial growth by binding to the 30S subunit of bacterial ribosomes and inhibiting protein synthesis. (medscape.com)
Imipenem/cilastatin combination inhibits bacterial cell wall synthesis by binding to one or more of the penicillin-binding proteins (PBPs). (medscape.com)
Linezolid inhibits bacterial protein synthesis by binding to bacterial 23S ribosomal RNA of the 50S subunit. (medscape.com)
Tigecycline inhibits bacterial protein by binding to the 30S ribosomal subunit. (medscape.com)
Doxycycline inhibits protein synthesis by binding with the 30S subunit. (medscape.com)
Ciprofloxacin inhibits bacterial DNA synthesis by binding to gyrase. (medscape.com)
Mechanistically, FSH, via binding to hepatic FSHRs, activates the Gi2α/β-arrestin-2/Akt pathway and subsequently inhibits the binding of FoxO1 with the SREBP-2 promoter, thus preventing FoxO1 from repressing SREBP-2 gene transcription. (nature.com)

By utilizing structural information obtained through X-ray crystallography or NMR spectroscopy, these tools allow efficient and affordable examination of large small-molecule databases and provide quantitative evaluation of the likelihood that a given protein-ligand interaction occurs. (ku.edu)
This method involves identifying "hotspot pharmacophores" from the protein-RNA interaction and using it as a template in ligand-based virtual screening. (ku.edu)
A dry ligand-binding cavity in a solvated protein. (lu.se)
Furthermore, it is important and challenging to accurately model the large entropic contribution to ligand-binding free energies. (lu.se)
This entropy largely stems from the fluctuation of the protein and ligand. (lu.se)
Additionally, we compared how MD and grand-canonical Monte Carlo (GCMC) can be used to assess dynamics and thermodynamics of protein-ligand binding for both buried and solvent-exposed binding sites. (lu.se)

The latest VOC, Omicron, carries 37 mutations in its spike protein, most of which lie within the receptor-binding domain (RBD), the target for neutralizing antibodies (nAbs). (news-medical.net)

In this paper, we implemented a novel deep neural network model, DeepRKE, which combines primary RNA sequence and secondary structure information to effectively predict RBP binding sites. (biomedcentral.com)
For instance, RNAcontext [ 12 ] uses position weight matrix (PWM) of RNA sequence and secondary structure profile to predict the binding preference of RBP. (biomedcentral.com)
GraphProt [ 14 ] adopts the form of graph coding to integrate RNA sequence and structure into the graph kernel to generate a feature vector of more than 3,000 dimensions, which is subsequently used as the input of an SVM model to predict RBP binding preference. (biomedcentral.com)
IONMF [ 15 ] proposes a feature representation method of orthogonal matrix eigendecomposition, which integrates the k-mer sequence, secondary structure, gene ontology (GO) information and region type as input into a machine learning model to predict binding sites. (biomedcentral.com)

Styrene oxide, a reactive metabolite of styrene formed via cytochrome P450 enzymes, has been reported to form covalent bonds with proteins, such as albumin and hemoglobin. (cdc.gov)

Olfactory sensors based on odor-binding proteins (OBPs) have been utilized to analyze pollutants in food and air samples, while their application for the detection of typical odor-causing compounds in drinking water is rarely reported, partly due to the lack of knowledge about the binding properties of odorants. (springeropen.com)

In this review, we assess the potential role of putative gut microbiota products in the etiopathogeny of Parkinson's disease, with a special emphasis on functional bacterial amyloid proteins, bacterial biosurfactants, endotoxins and short-chain fatty acids. (techscience.com)

I show that APPRAISE is capable of distinguishing between receptor-dependent and receptor-independent adeno-associated viral vectors and ranking various engineered proteins, such as miniproteins binding to the SARS-CoV-2 spike and nanobodies binding to a G-protein-coupled receptor. (caltech.edu)
These results validate and extend prior findings on the role of glycan bindings of viral spike protein in COVID-19. (biorxiv.org)

By conducting a chemical array screening, we found that MLPs bound compounds with indole- and quinazoline-like structures. (nih.gov)
Biophysical characterization of these compounds suggests that the majority of them selectively bind to the engineered ChxR variant. (ku.edu)

Human TSH is one of several glycoprotein hormones consisting of two non-covalently bound peptide chains: an a-chain, which is nearly identical in all, and a ß-chain, which is responsible for immunological and biological specificity. (cdc.gov)

Surprisingly, chinmo-/- GSCs rely on a new mechanism of competition in which they secrete the extracellular matrix protein Perlecan to selectively evict non-mutant GSCs and then upregulate Perlecan-binding proteins to remain in the altered niche. (lu.se)

They furthermore suggest therapeutic options using competitive glycan-binding agents such as IVM and may help elucidate rare serious adverse effects (AEs) associated with COVID-19 mRNA vaccines which use spike protein as the generated antigen. (biorxiv.org)

I chose ChxR, a homodimeric response regulator in Chlamydia, as the model protein to examine the feasibility of this strategy. (ku.edu)
The enzyme is composed of the heterotetrameric MoFe protein that is transiently associated with the homodimeric Fe protein . (bionity.com)
Nitrogenase is supplied reducing power when it associates with the reduced, nucleotide-bound homodimeric Fe protein. (bionity.com)

By examining the structure of such inhibitors, we can learn more about the properties of the substrate binding site on the carrier protein. (erowid.org)
Nitrogenase is able to bind acetylene and carbon monoxide, which are noncompetitive substrates and inhibitors , respectively. (bionity.com)
Farnesyl- and geranyl-pyrophosphates are competitive inhibitors. (lu.se)

A melatonin indicator was then created by integrating the repurposed receptor with a fluorescent protein. (caltech.edu)

The identification of RBP binding sites is a crucial step in understanding the biological mechanism of post-transcriptional gene regulation. (biomedcentral.com)
Therefore, the identification of RBP binding sites is crucial to understanding the mechanism of biological processes. (biomedcentral.com)
The exact mechanism of catalysis is unknown due to the difficulty in obtaining crystals of nitrogen bound to nitrogenase. (bionity.com)

Chromatography is a code of techniques used to purify as well as separate bimolecular substances such as proteins. (prnewswire.co.uk)

the subsequent binding of exogenous indole then reverts this conformational change and restores the native enzyme structure. (ku.edu)

Strong experience with protein biochemistry, molecular RNA analyses and cell culture is highly desirable. (eurosciencejobs.com)
The electrostatic potential of the central region of spike protein from these four lineages was studied through molecular modeling simulations. (biorxiv.org)
Glaxo Wellcome, Bureau d'Affaires du Québec, The Institut de Recherche en Santé unknown for most low-molecular-weight agents. (cdc.gov)

The PBI tests of the 1950s that estimated the TT4 concentration as "protein-bound iodide" were replaced in the 1960s first by competitive protein binding methods and later in the 1970s by radioimmunoassay (RIA) methods. (medscape.com)

To visualize new target proteins, a major drawback is usually the lack of sensitive and specific antibodies. (nature.com)
Antibodies can label immature or inactive proteins hence their usefulness to quantify functionally relevant pools of signaling molecules is also limited. (nature.com)
Development of polyclonal antibodies for the detection of styrene oxide modified proteins. (cdc.gov)
The purpose of this study is to develop polyclonal antibodies for the detection of styrene oxide cysteinyl protein adducts. (cdc.gov)
Competitive ELISA demonstrated that the resulting antibodies specifically recognized the styrene oxide-derived N-acetylcysteine adduct. (cdc.gov)
Competitive Western blots further indicated that these antibodies specifically recognized styrene oxide cysteinyl-protein adducts. (cdc.gov)
In conclusion, we successfully raised polyclonal antibodies to detect styrene oxide-derived protein/cysteine adducts. (cdc.gov)

The first project, focused on activation of protein function, is built on an approach developed by our laboratory that designs a de novo allosteric binding site directly into the catalytic domain of an enzyme. (ku.edu)

Dinitrogen, however, is a competitive substrate for acetylene. (bionity.com)

Circular RNAs (circRNAs) are closed, long, non-coding RNAs that are formed via direct reverse splicing of precursor mRNA that function by regulating RNA transcription and protein production, as well as sponging miRNAs ( 4 ). (spandidos-publications.com)
The transcription of COX-1 yields a 2.7-kilobase (kb) messenger ribonucleic acid (mRNA) that encodes a 576-residue, 65-kd protein. (medscape.com)
Conversely, the transcription of COX-2 yields a 4.5-kb mRNA that encodes a 70-kd protein with roughly 70-75% homology to the COX-1 protein. (medscape.com)

To achieve these goals, the successful candidate will employ cutting-edge protein and RNA biochemistry techniques, imaging and high-throughput sequencing to assess the impact of RNA binding on protein/RNA stability and function. (eurosciencejobs.com)
Resonance enhanced absorption (REA) nanocolor microfluidic devices are new promising bioassay platforms, which employ nanoparticle- (NP-) protein conjugates for the immunodetection of medically relevant markers in biologic samples such as blood, urine, and saliva. (hindawi.com)

My Ph.D. projects centered on using computational structural biology tools to develop protein engineering methods for targeted therapeutic delivery, emphasizing delivering molecules to the brain. (caltech.edu)
The report provides a snapshot of the global therapeutic landscape for E3 Ubiquitin Protein Ligase Mdm2 (Double Minute 2 Protein or Hdm2 or Oncoprotein Mdm2 or p53 Binding Protein Mdm2 or MDM2 or EC 6.3.2. (researchandmarkets.com)

Analysis of protein dynamics simulations by a stochastic point process approach. (lu.se)

This work addresses contaminant detection both in clinical situations and during protein purification of pharmaceutical products. (lu.se)

Second, I describe the development of Automated Pairwise Peptide-Receptor AnalysIs for Screening Engineered proteins (APPRAISE). (caltech.edu)

SCOPe: Structural Classification of Proteins - extended. (berkeley.edu)

Experimental findings for SARS-CoV-2 related to the glycan biochemistry of coronaviruses indicate that attachments from spike protein to glycoconjugates on the surfaces of red blood cells (RBCs), other blood cells and endothelial cells are key to the infectivity and morbidity of COVID-19. (biorxiv.org)

Activator protein-1 and nuclear factor-kappaB, crucial transcription factors involved in COX-2 expression, were activated by UVB and delphinidin abolished this activation. (oregonstate.edu)
Using the Drosophila testis model, we show that germline stem cells (GSCs) lacking the transcription factor Chinmo gain a competitive advantage for niche access. (lu.se)

E3 ubiquitin-protein ligase Mdm2 is a protein encoded by the MDM2 gene. (researchandmarkets.com)

Carbamylation of plasma did not influence quinidine protein binding, but resulted in decreased salicylate binding. (nih.gov)

Commercially available pesticides amisulbrom and pyrifluquinazon, which possess such structures, successfully inhibited the binding of MLPs to pyrene and dieldrin in vitro. (nih.gov)

In contrast, when Omicron spike protein was used, P2G3 was three-fold more potent than P5C3. (news-medical.net)
The results of these experiments were, first, that spike protein from these four lineages of SARS-CoV-2 induced HA. (biorxiv.org)
Omicron induced HA at a significantly lower threshold concentration of spike protein than for the three prior lineages and was much more electropositive on its central spike protein region. (biorxiv.org)
IVM blocked HA when added to RBCs prior to spike protein and reversed HA when added afterwards. (biorxiv.org)

Major latex-like proteins (MLPs), found in the Cucurbitaceae family, play an important role in the contamination by binding to these hydrophobic pollutants. (nih.gov)
Herein, we competitively inhibited the binding of MLPs to hydrophobic pollutants, pyrene and dieldrin, in roots by using MLP binding pesticides. (nih.gov)

This is because binding of dinitrogen prevents acetylene binding, and acetylene requires only one electron to be reduced, and it does not inhibit. (bionity.com)

A sample is added to a reaction vessel with goat anti-hTSH-alkaline phosphatase conjugate, buffered protein solution, and paramagnetic particles coated with immobilized mouse monoclonal anti-hTSH antibody. (cdc.gov)
After incubation in a reaction vessel, materials bound to the solid phase are held in a magnetic field while unbound materials are washed away. (cdc.gov)
Sample is added to a reaction vessel with a stripping agent to dissociate T3 from the binding proteins. (cdc.gov)
A sample is added to a reaction vessel with anti-thyroxine antibody, thyroxine-alkaline phosphatase conjugate, and paramagnetic particles coated with goat anti-mouse capture antibody and a stripping agent to dissociate all T4 from binding proteins. (cdc.gov)
No cross reaction was observed from the native protein. (cdc.gov)

Especially, deep learning is increasingly used in the bioinformatics field by virtue of its ability to learn generalized representations from DNA and protein sequences. (biomedcentral.com)

With the increasing volume of verified RBP binding sites, quite a few studies focused on developing computational prediction models based on the known RBP binding sites. (biomedcentral.com)
This computational pipeline predicts the receptor binding propensity of engineered proteins based on competitive modeling and physics-grounded analysis. (caltech.edu)

Small molecules readily penetrate tissue preparations and bind their targets with known stoichiometry. (nature.com)

On the protein level, out of 5,150 proteins identified, 393 were significantly upregulated and 227 were significantly downregulated. (frontiersin.org)
The most significantly upregulated process was the response to heat stress (mainly heat shock proteins, HSPs). (frontiersin.org)

The first layer, called the Stern layer, corresponds to the primary electric surface potential or Stern potential (caused by protonation/deprotonation reactions on the surface) and ions from the bulk electrolyte strongly bound to its surface. (materialstoday.com)

Time scales of water dynamics at biological interfaces: peptides, proteins and cells. (lu.se)

The ability to modulate protein function using exogenous small molecules is a longstanding goal in chemical biology. (ku.edu)

In addition to parameters such as size and shape, chemical functionalities on the surface seem to play a critical role in binding to cell membrane and subsequent cellular uptake [2] and [3] . (materialstoday.com)

The valuable information to the binding of OBP2a to typical odorants in this study would provide a theoretical foundation for the development of OBP-based odor detection biosensors to achieve quick detection in drinking water, further helping the improvement of water treatment processes in the water industry. (springeropen.com)

and demonstrated broad PharmacoSTORM applicability for three protein classes and for cariprazine, a clinically approved antipsychotic and antidepressant drug. (nature.com)
Taken together, my work aims to not only apply rational design strategies to specific proteins, but also demonstrate their general applicability. (ku.edu)

Our results demonstrate that the pesticides binding to MLPs competitively inhibited the binding of MLPs to pyrene and dieldrin in roots, resulting in the reduction of overall contamination. (nih.gov)

Also, urinary loss of both vitamin D and vitamin D binding protein (VDBP) has been reported in these individuals. (karger.com)

One crucial property that is not yet competitive for Fe carbene photosensitisers is how long they stay in the excited state, i.e. their lifetime. (lu.se)

Distribution is generally uneven because of differences in blood perfusion, tissue binding (eg, because. (msdmanuals.com)

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