Common AntigensPTPRCT200IsoformsAntibodyRECEPTORSLymphocytesB220Erythrocytes and plateletsIsoform expressionMoleculesSignal transductionKinasesGeneImmuneBone marrowCellsPlateletsCloneGlycoproteinAnti-CD45Oncogenic transformationTransmembrane tyrosine phosphataseReceptor-mediated activationCD43IsotypeAffinityAutoimmuneMultiple sclerosis susceptibilityRegulatorCellPeptidesDifferentiationExpressionReacts
Common Antigens1
- Cobbold SP, Hale G, Waldmann H. Non-lineage, LFA-1 family, and leucocyte common antigens: new and previously defined clusters. (bdbiosciences.com)
PTPRC12
- The protein encoded by this gene was identified as a transmembrane phosphoprotein specifically associated with tyrosine phosphatase PTPRC/CD45, a key regulator of T- and B-lymphocyte activation. (wikipedia.org)
- The interaction with PTPRC may be required for the stable expression of this protein. (wikipedia.org)
- CD45, previously called LCA (leukocyte common antigen), T200, or Ly5 in mouse, is member C of the class 1 (receptor-like) protein tyrosine phosphatase family (PTPRC) (1, 2). (rndsystems.com)
- PTPRC/CD45: Protein Tyrosine Phosphatase Receptor Type C, CD45 antigen. (ox.ac.uk)
- We report here that a homozygous 6-bp deletion in the gene encoding CD45 (PTPRC, gene map locus 1q31-32), which results in a loss of glutamic acid 339 and tyrosine 340 in the first fibronectin type III module of the extracellular domain of CD45, is associated with failure of surface expression of CD45 and SCID. (aston.ac.uk)
- The CD45 antigen (Receptor-type tyrosine-protein phosphatase C, PTPRC) is a protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. (otwobio.com)
- Full length human recombinant protein of human PTPRC(NP_002829) produced in HEK293T cell. (origene.com)
- PTPRC (CD45) is a transmembrane tyrosine phosphatase that is present on all leukocytes. (ximbio.com)
- PTPRC regulates the threshold of T cell antigen receptor (TCR) signaling through dephosphorylation of protein tyrosine kinases (e.g. (ximbio.com)
- We recently identified a polymorphism in exon 6 (A138G) of the gene encoding CD45 (PTPRC) that results in altered CD45 splicing. (ox.ac.uk)
- CD45, also referred to as CD45R and PTPRC (Protein tyrosine phosphatase receptor type C), has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. (nsjbio.com)
- pathways involving PTPRC protein from the Biocarta Pathways dataset. (maayanlab.cloud)
T2002
- CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. (biolegend.com)
- CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. (biolegend.com)
Isoforms17
- Recognizes all CD45 isoforms as well as the known mouse CD45 alloantigens (CD45-1/Ly 5a and CD45-2/Ly 5b) (15). (rndsystems.com)
- CD45 has several isoforms, expressed according to cell type, developmental stage and antigenic exposure (1‑5). (rndsystems.com)
- The mouse monoclonal antibody HI30 recognizes an extracellular epitope on all isoforms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets. (exbio.cz)
- CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. (thermofisher.com)
- CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. (thermofisher.com)
- Combinations of CD45 isoforms are crucial for immune function and disease. (exbio.cz)
- The CD45 molecules are single chain integral membrane proteins comprising at least five isoforms ranging from 180 to 220 kDa. (beckman.com)
- The non-restricted CD45, Leucocyte Common Antigen (LCA) consists of an extracellular sequence, proximal to the membrane, which is common to all CD45 isoforms. (beckman.com)
- All the monoclonal antibodies that belong to the CD45 cluster react with this part of the antigen and are able to recognize all CD45 isoforms. (beckman.com)
- Several different splice isoforms of the extracellular domain of CD45 are expressed on T cells, and the isoform expressed depends on the particular subpopulation of cell, the state of cell maturation, and whether the cells have been exposed to antigen. (lsbio.com)
- With their broad cell distribution, CD45 isoforms are critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. (cytekbio.com)
- In particular, we examined expression of these phenotypic markers in relation to the expression of CD45 isoforms. (ox.ac.uk)
- In some individuals, expansions of antigen-specific CD8+ T lymphocytes bearing specific TCR Vbeta chains were restricted to cells of particular CD45 isoforms. (ox.ac.uk)
- Expression of different patterns of alternatively spliced CD45 isoforms is associated with distinct functions. (ox.ac.uk)
- In this study we show that the A138G polymorphism is the cause of altered CD45 isoform expression, promoting splicing towards low molecular weight CD45 isoforms. (ox.ac.uk)
- Multiple isoforms of CD45 are distributed throughout the immune system and arise due to alternative splicing of exons located in the N-terminus. (nsjbio.com)
- This antibody will bind to all CD45 isoforms. (nsjbio.com)
Antibody20
- ALCL was recognized in 1985, when tumor cells consistently demonstrated labeling by the monoclonal antibody Ki-1, a marker later shown to recognize the CD30 antigen. (medscape.com)
- The CD45 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. (biolegend.com)
- Left Plot) or BD Horizon BV480 Mouse Anti-Human CD45 antibody (Cat. (bdbiosciences.com)
- This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. (thermofisher.com)
- Purified antibody is conjugated with activated tandem dye of activated Peridinin-Chlorophyll Protein-Cyanine 5.5 (PerCP-Cy™5.5) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. (exbio.cz)
- 1 2 Cell surface antigens CD38 and CD138 can be used to distinguish normal cells from clonal plasma cells, but more extensive use of immunophenotyping has been limited by a lack of universally accepted markers of MM. 3-5 Once diagnosed, the current standard-of-care for MM includes immunomodulatory drugs, proteasome inhibitors, steroids, and antibody therapies. (bmj.com)
- Laboratories), and mouse anti-human monoclonal antibody against CD45 (leukocyte common antigen) (Dako) at 4C overnight (14-18 hours). (cdc.gov)
- To test the specificity of the staining, negative controls using protein block reagent in replacement of the lectin, and isotype-matched control in replacement of the primary antibody were included. (cdc.gov)
- As control we used a human leukocyte antigen DR (HLA DR) antibody with equal inhibitory capacities on cell proliferation. (biomedcentral.com)
- The antibody MEM-143 reacts with a protein determinant of CD45RB, a 180-240 kDa single chain type I membrane glycoprotein, variant of CD45 (CD45RB isoform). (lsbio.com)
- The UCHL1 antibody reacts with the human CD45 isoform known as CD45RO, a protein tyrosine phosphatase of ≥ 220 kDa. (cytekbio.com)
- IHC testing of FFPE human tonsil with anti-CD45 antibody (clone SPM570). (nsjbio.com)
- This anti-CD45 antibody is available for research use only. (nsjbio.com)
- Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms. (nsjbio.com)
- Due to variation in protocol and secondary antibody used, the anti-CD45 antibody may need to be titered for optimal performance. (nsjbio.com)
- Neoplastic cells from a T cell lymphoma were used as the immunogen for the anti-CD45 antibody. (nsjbio.com)
- Anti-CD45 antibody (with azide) can be stored at 2-8oC. (nsjbio.com)
- The purpose of this study was to determine if labeling leukocytes with a monoclonal antibody specific to leukocyte common antigen (CD45) was superior to labeling with LDS-751 and to determine the effect of sample fixation on a mouse whole blood preparation for flow cytometry. (arizona.edu)
- We conclude that labeling leukocytes with monoclonal antibody CD45 in a mouse whole blood preparation is preferable, as it provides improved measurement of leukocyte indices compared to LDS-751. (arizona.edu)
- The position of bands on the nitrocellulose strips allows this antibody reactivity to be associated with specific viral antigens. (cdc.gov)
RECEPTORS2
- Endometrium from pregnant heifers had greater mRNA abundance for the inhibitory molecules, CD274 and lymphocyte activating gene 3 (LAG3), and greater cytotoxic T lymphocyte-associated protein 4 (CTLA4), molecules that can interact with receptors on antigen-presenting cells and induce lymphocyte tolerance. (psu.edu)
- The first step of the adaptive immune response involves the interaction of T cells that express T-cell receptors (TCRs) with peptide-loaded major histocompatibility complexes expressed by antigen-presenting cells (APCs). (ox.ac.uk)
Lymphocytes11
- CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. (biolegend.com)
- CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. (exbio.cz)
- The non-restricted CD45 epitope is present on the surface of all human leucocytes, lymphocytes, eosinophils monocytes, basophils and neutrophils by order of decreasing level of expression. (beckman.com)
- CD45-deficient mice are severely immunodeficient and have very few peripheral T lymphocytes. (aston.ac.uk)
- CD45 antigen is expressed at high levels on all hematopoietic cells including T and B lymphocytes, monocytes, granulocytes, NK cells and dendritic cells, but is not expressed on non-hematopoietic cells. (woofahs.com)
- We therefore hypothesised that we will be able to induce anergy in CD45-treated lymphocytes. (biomedcentral.com)
- Moreover, these anergic lymphocytes were extremely sensitive for protein kinase C-mediated apoptosis, indicating that immunosuppressive mechanisms are inter-related. (biomedcentral.com)
- Ex vivo analysis of phenotype and TCR usage in relation to CD45 isoform expression on cytomegalovirus-specific CD8+ T lymphocytes. (ox.ac.uk)
- Immunity against CMV comprises a large population of CD8+ T lymphocytes with heterogeneous potential, a spectrum in which CD45 isoform expression may play a central role. (ox.ac.uk)
- The CD45 antigen is a haemopoietic cell specific tyrosine phosphatase essential for antigen receptor mediated signalling in lymphocytes. (ox.ac.uk)
- BD Tritest™ CD3 fluorescein isothiocyanate (FITC)/CD4 phycoerythrin (PE)/CD45 peridinin chlorophyll protein (PerCP) is a three-color direct immunofluorescence reagent for use with a suitably equipped flow cytometer to identify and determine the percentages and absolute counts of mature human T lymphocytes (CD3 + ) and helper/inducer (CD3 + CD4 + ) T-lymphocytes subsets in erythrocyte-lysed whole blood. (bdbiosciences.com)
B2201
- Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA and CD45RB. (cytekbio.com)
Erythrocytes and platelets2
- It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. (biolegend.com)
- CD45 has also been reported to react weakly with mature blood erythrocytes and platelets. (woofahs.com)
Isoform expression3
- Cosenza-Nashat MA, Kim MO, Zhao ML, Suh HS, Lee SC: CD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis. (exbio.cz)
- Although the detailed composition of these pools is not completely understood, there is known to be heterogeneity, in particular of CD45 isoform expression. (ox.ac.uk)
- We found that a spectrum of phenotypes exists stably, from CD45R0(high)/RA(low) through CD45RA(high)/R0(low), and that expression of other surface markers such as CD28 and CD62L, and also TCR usage, may vary in parallel with CD45 isoform expression. (ox.ac.uk)
Molecules6
- Interestingly, it has been shown that platelets contain a broad spectrum of RNA molecules, including, in addition to mRNAs and miRNAs, also pre-mRNAs and a role of mRNA splicing in regulation of platelet protein synthesis has been proposed 18 , 19 . (nature.com)
- We are currently investing this problem by studying the organisation of key molecules, such as CD45, TCR, CD4 and pMHC, on the surface of T cells and antigen presenting cells during the early stages of an immune response. (lu.se)
- Examples of this includes measuring the extremely weak interaction between the immune cell proteins CD4 and pMHC class II, 4 and we have also shown how auxiliary binding molecules can both increase and decrease the apparent affinity of TCR to pMHC depending on the relative protein densities. (lu.se)
- Function of T helper cells: Antigen presenting cells ( APCs ) present antigen on their Class II MHC molecules ( MHC2 ). (wikidoc.org)
- Important insights that have been obtained include (i) how protein flexibility and coverage might affect the effective heights of these molecules, (ii) the height of proteins on the membrane as a key parameter determining how they will distribute in cell-cell contacts, and (iii) how repulsive interactions between the extracellular parts of the proteins influences protein aggregation and distribution. (ox.ac.uk)
- CMV-specific CTLs were identified by flow cytometry using fluorochrome-labeled monoclonal antibodies against CD3, CD8, CD45 molecules, viability reagent and tetramers consisting of in-house produced MHC class I monomers loaded with one of two immunodominant epitopes of viral pp65 protein (NLVPMVATV (NLV) and TPRVTGGGAM (TPR) peptides that are present in HLA-A*02 and -B*07 antigens, respectively, bound to phycoerythrin-conjugated streptavidin. (cttjournal.com)
Signal transduction3
- CD45 plays a key role in TCR and BCR signal transduction. (biolegend.com)
- Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. (exbio.cz)
- CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions. (thermofisher.com)
Kinases8
- CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. (biolegend.com)
- CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. (exbio.cz)
- CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. (thermofisher.com)
- It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. (origene.com)
- Outcomes Inhibitions of PDGF receptor kinase, the docking proteins element Src-family kinases, as well as the success component PI3K all eradicated PDGF-stimulated ROS creation and corroborated using the suppressed cell development. (thetechnoant.info)
- signaling in the zoom lens epithelial cells, where concerted efforts from the upstream the different parts of PDGF receptor kinase, Src-family kinases, PI3K, Rac, and Ras protein are needed. (thetechnoant.info)
- Among the goals for ROS in vivo may be the reversible oxidation of phosphatases, which as well as proteins tyrosine kinases are in charge of maintaining a standard proteins tyrosine phosphorylation-dephosphorylation homeostasis in cell signaling in vivo [5,14]. (thetechnoant.info)
- Included in these are Src family members kinases, phosphatidylinositol-3-kinase (PI3K), phospholipase C (PLC) and little GTP-binding proteins Ras [34-36]. (thetechnoant.info)
Gene6
- Protein tyrosine phosphatase receptor type C-associated protein is an enzyme that in humans is encoded by the PTPRCAP gene. (wikipedia.org)
- And they showed that by putting Pax7 gene back into CD45:Sca1 cells taken from uninjured muscle, they were able to generate cells that readily differentiate into muscle cells. (ohri.ca)
- The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. (origene.com)
- The protein encoded by this gene represents the beta subunit and is a type I membrane protein. (cancer-genetics.org)
- What does this gene/protein do? (cancer-genetics.org)
- What pathways are this gene/protein implicaed in? (cancer-genetics.org)
Immune10
- They are generated by immunizing an animal with an antigen to elicit an immune response. (cellsignal.com)
- This is the second description of a clinically relevant CD45 mutation, provides direct evidence for the importance of CD45 in immune function in humans, and suggests that abnormalities in CD45 expression are a possible cause of SCID in humans. (aston.ac.uk)
- Furthermore, a large percentage of uterine immune cells coexpressed these proteins. (psu.edu)
- Investigation of the organisation and function of key membrane proteins in the initiation of an adaptive immune response. (lu.se)
- An important step is the triggering of T cells to start an immune response, which takes place when a T cell contacts an antigen presenting cell. (lu.se)
- However, how this is controlled by different membrane proteins and the molecular interactions involved to initiate an adaptive immune response are for many instances lacking. (lu.se)
- 3 We are also using these systems to measure two-dimensional binding kinetics of various protein-protein interactions between cells in the adaptive immune system. (lu.se)
- Disease associations and altered immune function in CD45 138G variant carriers. (ox.ac.uk)
- During an immune response, professional antigen-presenting cells (APCs) endocytose (absorb) foreign material (typically bacteria or viruses ), which undergoes processing , then travel from the infection site to the lymph nodes . (wikidoc.org)
- The combination of electrophoretic separation of complex mixtures of antigens with the highly sensitive immunoblotting technique has been useful in characterizing the antigenic profile of HIV-1 and describing the immune response to this virus in exposed or infected persons. (cdc.gov)
Bone marrow4
- CD45 antibodies react with leucocyte progenitors in bone marrow. (beckman.com)
- Diagnosis of MM is based on morphological features, imaging studies, analysis of myeloma cell-produced proteins, and testing of the blood, urine, and bone marrow (BM). (bmj.com)
- Murine studies revealed that Nell-1 haploinsufficiency results in marked reductions in the numbers of Sca-1+CD45-CD31- bone marrow MPCs associated with low bone mass. (nih.gov)
- Conversely, rhNELL-1 systemic administration in mice showed a marked anabolic effect accompanied by increased numbers of Sca-1+CD45-CD31- bone marrow MPCs. (nih.gov)
Cells27
- Anaplastic large cell lymphomas (ALCLs) are distinguished from other lymphomas by their anaplastic cytology and constant membrane expression of the CD30 antigen (an activation marker for B or T cells). (medscape.com)
- It is a variably glycosylated 180‑220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1‑3). (rndsystems.com)
- CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds (3, 6‑8). (rndsystems.com)
- Confocal immunofluorescent analysis of Raw 264.7 (left, positive) and C2C12 (right, negative) cells using CD45 (D3F8Q) Rabbit mAb (green). (cellsignal.com)
- CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. (thermofisher.com)
- Bijian K, Zhang L, Shen SH: Collagen-mediated survival signaling is modulated by CD45 in Jurkat T cells. (exbio.cz)
- A certain population of these stem cells, which are recognized by the cell surface proteins CD45 and Sca1 (stem cell antigen-1), is involved in normal muscle tissue repair, but is only triggered into the muscle cell development pathway by injury. (ohri.ca)
- The group also demonstrated that a protein called Pax7 is required to turn adult stem cells into muscle cells during regeneration. (ohri.ca)
- In earlier work, the researchers showed that that CD45:Sca1 cells taken from regenerating muscle in mice lacking Pax7 could not become muscle cells. (ohri.ca)
- Michael Rudnicki explains "This work demonstrates that Pax7 represents a key protein in the chain of events that directs stem cells to repair muscle. (ohri.ca)
- MS-based validation using primary specimens detected 30 proteins with significantly higher abundance in patient MM cells than controls. (bmj.com)
- Nine of these proteins were identified as potential immunotherapeutic targets, including five that were validated by FCM, confirming their expression on the cell surface of primary MM patient cells. (bmj.com)
- Prolonged rhNELL-1 protein release was achieved using an apatite-coated β-tricalcium phosphate carrier, resulting in a local influx of stem cell antigen-1-positive (Sca-1+) mesenchymal progenitor cells (MPCs), and complete osseous fusion across all samples (100% spinal fusion rate). (nih.gov)
- Required for T-cells activation through the antigen receptor. (lu.se)
- Compared to cyclic heifers, CD45 + uterine cells from pregnant heifers exhibited greater degranulation. (psu.edu)
- Transiently transfected cells with plasmid from little GTP-binding proteins Rac N17 or Ras N17 reduced PDGF actions in ROS era, cell proliferation and MAP kinase activation, while cells with Rac V12 improved the PDGF impact. (thetechnoant.info)
- By using SLBs it is possible to replace one of the cells in the contact with an artificial cell membrane, the SLB, which can be made to contain a well-controlled mix of the membrane proteins that are investigated. (lu.se)
- Mature T h cells are believed to always express the surface protein CD4 . (wikidoc.org)
- Antigen presentation stimulates T cells to become either "cytotoxic" CD8+ cells or "helper" CD4+ cells. (wikidoc.org)
- Following T cell development , matured, naïve (meaning they have never been exposed to the antigen to which they can respond) T cells leave the thymus and begin to spread throughout the body, including the lymph nodes . (wikidoc.org)
- Class II MHC proteins are generally only found on the surface of professional antigen-presenting cells (APCs). (wikidoc.org)
- Professional antigen presenting cells are primarily dendritic cells , macrophages and B cells , although dendritic cells are the only cell group that expresses MHC Class II constitutively (at all times). (wikidoc.org)
- Some APCs also bind native (or unprocessed) antigens to their surface, such as follicular dendritic cells , but unprocessed antigens do not interact with T cells and are not involved in their activation. (wikidoc.org)
- Once at the lymph nodes, the APC begins to present antigen peptides that are bound to Class II MHC, allowing CD4 + T cells that express specific TcR's against the peptide/MHC complex to activate. (wikidoc.org)
- CD45 is expressed in most nucleated cells of hematopoietic origin, and is an essential regulator of T and B cell antigen receptor signalling. (assaysolution.com)
- Taking advantage of the diverse γδ TCR repertoire or other ligand-receptor interactions, γδ T cells can recognize a broad spectrum of tumor-associated antigens (TAAs) in a major histocompatibility complex (MHC)-independent manner, thereby activating downstream pleiotropic effects. (explorationpub.com)
- Leukocyte cell suspension after preliminary red blood cells lysis were incubated with a protein tyrosine kinase inhibitor (Dasatinib) for 1 hour at 37°С in order to increase surface expression of both TCR and CD8. (cttjournal.com)
Platelets2
- The evidence that platelets are capable of de novo protein synthesis in response to stimuli raised the issue of how megakaryocyte-derived mRNAs are regulated in these anucleate cell fragments. (nature.com)
- Evidence that platelets are capable of de novo protein synthesis 17 also raised the issue of whether resident mRNAs are regulated in circulating platelets and, if so, why. (nature.com)
Clone1
- C57BL/6 splenocytes were stained with anti-mouse CD45 (clone I3/2.3) APC/Cyanine7 (filled histogram), or rat IgG2b APC/Cyanine7 isotype control (open histogram). (biolegend.com)
Glycoprotein1
- CD45RB is a splice isoform of CD45 (also called protein tyrosine phosphatase, receptor type, C), a glycoprotein receptor that is required for T-cell activation. (lsbio.com)
Anti-CD454
- Anti-CD45 positively stains the majority of lymphoid neoplasms, and is highly indicative of lymphoid origin. (assaysolution.com)
- The samples were then fixed with paraformaldehyde or diluted with PBS followed by incubation with 5 μg/ml PerCP-conjugated anti-CD45, 5 μg/ml FITC-conjugated anti-CD11b, or 80 μM dichlorofluorescein diacetate. (arizona.edu)
- We found that samples labeled with LDS-751 demonstrated decreased fluorescence intensity for granulocyte CD11b expression and ROS production compared to samples labeled with anti-CD45. (arizona.edu)
- In addition, sample fixation decreased mean fluorescence intensity in samples labeled with either LDS-751 or anti-CD45. (arizona.edu)
Oncogenic transformation2
- CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. (biolegend.com)
- Cluster of differentiation 45 (CD45), also known as leukocyte common antigen (LCA), is a member of the protein tyrosine phosphatase (PTPase) family which is known to regulate a variety of cellular processes including cell growth, differentiation, the mitotic cycle, and oncogenic transformation. (assaysolution.com)
Transmembrane tyrosine phosphatase1
- CD45 is an abundant transmembrane tyrosine phosphatase, expressed on all leukocytes, and is required for efficient lymphocyte signaling. (aston.ac.uk)
Receptor-mediated activation1
- Along with other members of the PTP family, it regulates a number of cellular processes including cell differentiation, growth and mitotic cycle, and is an essential regulator of B- and T-cell antigen receptor-mediated activation. (nsjbio.com)
CD432
- Sixty percent of cases express 1 or more T-cell antigens (CD3 + , CD43, or CD45RO). (medscape.com)
- If this is true, a full understanding of how close contact formation leads to signaling would require insights into the structures of, and interactions between, large membrane proteins like CD45 and other proteins forming the glycocalyx, such as CD43. (ox.ac.uk)
Isotype2
- Two-parameter flow cytometric contour plots showing the correlated expression of CD45 (or Ig Isotype control staining) versus side-light scatter (SSC-A) signals were derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. (bdbiosciences.com)
- Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is CD45 (D3F8Q) Rabbit mAb. (cellsignal.com)
Affinity1
- Purified by protein-A affinity chromatography. (lsbio.com)
Autoimmune1
- CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders (6, 7). (rndsystems.com)
Multiple sclerosis susceptibility1
- Genetic variations in CD45 are involved in multiple sclerosis susceptibility. (otwobio.com)
Regulator1
- CD45 acts as a positive regulator of T-cell coactivation upon binding to DPP4. (otwobio.com)
Cell22
- In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP-1/CD148 (11‑14). (rndsystems.com)
- CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. (biolegend.com)
- Immunoprecipitation of CD45 protein from BaF3 cell extracts. (cellsignal.com)
- CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. (thermofisher.com)
- We investigated the first SCID patient to be described with minimal cell surface expression of the leukocyte common (CD45) Ag. (aston.ac.uk)
- Defects in CD45 are a cause of severe combined immunodefiency autosomal recessive T-cell negative/B-cell-positive/NK-cell-positive (T-B+NK+ SCID). (otwobio.com)
- Glycans, either alone or complexed with glycan-binding proteins, can deliver intracellular signals or control extracellular processes that promote initiation, execution and resolution of cell death programs. (nature.com)
- Herein, we review the role of glycans and glycan-binding proteins as essential components of the cell death machinery during physiologic and pathologic settings. (nature.com)
- At that time, glycobiology, which is the study of carbohydrates and their recognition by motif-specific carbohydrate-binding proteins or lectins, lagged far behind the studies that defined the structural and cellular biology of cell death. (nature.com)
- This approach was recently used by me and collaborators to study how exclusion of the long phosphatase CD45 from SLB/T-cell contacts can initiate T-cell signalling without any antigens, 1,2 and how the model membrane itself can induce signaling. (lu.se)
- (A) A schematic illustration showing specific binding between the proteins CD2 on a T cell with fluorescently-labelled CD58 in an SLB. (lu.se)
- The T cell receptor (TcR) consists of both constant and variable regions, the latter of which determines what antigen the T cell can respond to. (wikidoc.org)
- Dimensions and Interactions of Large T-Cell Surface Proteins. (ox.ac.uk)
- Recent findings suggest that one of the key events is the exclusion of the large receptor-type tyrosine phosphatase CD45, from close contacts formed at sites of T-cell/APC interaction. (ox.ac.uk)
- Structural insights into the overall dimensions of these proteins using crystallographic methods are hard to obtain, and their conformations on the cell surface are also unknown. (ox.ac.uk)
- Several imaging-based optical microscopy techniques have however been developed for analyzing protein dimensions and orientation on model cell surfaces with nanometer precision. (ox.ac.uk)
- Leucocyte Typing IV: White Cell Differentiation Antigens. (bdbiosciences.com)
- Clevers H, Alarcón B, Wileman T, Terhorst C. The T cell receptor/CD3 complex: a dynamic protein ensemble. (bdbiosciences.com)
- Thymus-dependent membrane antigens in man: inhibition of cell-mediated lympholysis by monoclonal antibodies to TH2 antigen. (bdbiosciences.com)
- However, an absence of CD45 does not rule out lymphoid tumors, as certain types of neoplasms lack CD45, such as Hodgkin's lymphoma, some T-cell lymphomas, and some leukemias. (assaysolution.com)
- Fixing samples with formaldehydes increases cell permeability and causes surface protein cross-linking that may alter staining of both intra- and extracellular markers. (arizona.edu)
- The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)
Peptides1
- The antigens that bind to MHC proteins are always short peptides , 8-10 amino acids long for MHC Class I, and up to 25 or so for MHC Class II. (wikidoc.org)
Differentiation1
- Bernard A, Boumsell L, Hill C. Joint report of the first international workshop on human leucocyte differentiation antigens by the investigators of the participating laboratories: T2 protocol. (bdbiosciences.com)
Expression3
- This includes the expression of distinct antigens on subsets of MSCs, which was utilized recently to define and separate functionally different subsets from bulk MSCs. (hindawi.com)
- Multiparameter flow cytometric analysis of CD45 expression on human peripheral blood leucocytes. (bdbiosciences.com)
- Scope includes mutations and abnormal protein expression. (cancer-genetics.org)
Reacts1
- Mouse monoclonal to CD45.4AA9 reacts with CD45, a 180-220 kDa leukocyte common antigen (LCA). (woofahs.com)