Proteins 1966 vesicular stomatitis virus vesicular stomatitis indiana virus. Medical search. Frequent questions

Budding of the SARS-CoV occurs in the Golgi apparatus [ 4 ] and results in the incorporation of the envelope spike glycoprotein into the virion. (biomedcentral.com)
The assay uses a recombinant vesicular stomatitis virus expressing NiV glycoprotein and a fluorescent protein. (cdc.gov)

There are four structural viral proteins, of which VP1 has been shown to elicit protective immunity in cattle ( Bachrach 1977 ). (wikisource.org)

217(12): 1957-1966, 2018 05 25. (bvsalud.org)

CCHFV has a wide geographic distribution, and cases have been reported in Africa, Asia, the Middle East, and Europe. (cdc.gov)

The first report of VES was in 1932 in California ( Traum 1934 ), although it was initially thought to be FMD. (wikisource.org)
Chloroquine, a 9-aminoquinoline that was identified in 1934, is a weak base that increases the pH of acidic vesicles. (biomedcentral.com)

Brown is an Academic Editor of Advances in Virology. (wikipedia.org)

Viral RNA polymerase, a nonstructural protein, has been termed "virus infection associated antigen" (VIAA) for diagnostic purposes but is more accurately termed "FMDV nonstructural protein 3D. (wikisource.org)

No dif- quitoes has been estimated by using artificial saliva collec- ferences in survival were detected, regardless of the dose or infection route. (cdc.gov)
Designing pathogenesis studies for arboviruses that lection and real-time reverse transcription (RT)-PCR esti- accurately simulate natural infection requires quanti- mate that Culex pipiens pipiens saliva contains an average fying the amount of virus transmitted. (cdc.gov)
Vector saliva enhances infection with many pathogens virus delivered in mosquito saliva. (cdc.gov)
They can be caused by infection with the viruses of foot-andmouth disease (FMD), vesicular stomatitis (VS), vesicular exanthema of swine (VES), and swine vesicular disease (SVD). (wikisource.org)
VS was recognized in horses and cattle in the United States during World War I ( Cotton 1927 ), but infection in swine was not reported until 1943 ( Schoening 1943 ). (wikisource.org)
This may negatively influence the virus-receptor binding and abrogate the infection, with further ramifications by the elevation of vesicular pH, resulting in the inhibition of infection and spread of SARS CoV at clinically admissible concentrations. (biomedcentral.com)
Favorable inhibition of virus spread was observed when the cells were either treated with chloroquine prior to or after SARS CoV infection. (biomedcentral.com)
Due to the severity of SARS-CoV infection, the potential for rapid spread of the disease, and the absence of proven effective and safe in vivo inhibitors of the virus, it is important to identify drugs that can effectively be used to treat or prevent potential SARS-CoV infections. (biomedcentral.com)
In this report, we describe the identification of chloroquine as an effective pre- and post-infection antiviral agent for SARS-CoV. (biomedcentral.com)
Chloroquine can affect virus infection in many ways, and the antiviral effect depends in part on the extent to which the virus utilizes endosomes for entry. (biomedcentral.com)
Together with data presented here, showing virus inhibition in cell culture by chloroquine doses compatible with patient treatment, these features suggest that further evaluation of chloroquine in animal models of SARS-CoV infection would be warranted as we progress toward finding effective antivirals for prevention or treatment of the disease. (biomedcentral.com)
This report describes generation of a recombinant Sudan virus and demonstrates its utility by quantifying antibody cross-reactivity between Ebola and Sudan virus glycoproteins after human infection or vaccination with a licensed Ebola virus vaccine. (cdc.gov)

Brown has worked widely on vesicular stomatitis virus, herpes simplex virus, and human papilloma virus. (wikipedia.org)

In 2022, Sudan virus re-emerged causing more than 160 confirmed and probable cases. (cdc.gov)

Hands on experience in techniques involving recombinant DNA technology such as preparation of plasmid DNA, transformation of bacteria by plasmid DNA, characterization of plasmids, cloning of DNA fragments into vectors, vaccinia virus, hybridization and electrophoretic procedures. (sprayprotectgo.com)
The recombinant virus propagates as a replication-competent virus in a cell line constitutively expressing NiV fusion protein, but it is restricted to a single round of replication in wild-type cells. (cdc.gov)

tural proteins to form the virion. (what-when-how.com)

Brown is most known for his work in the field of molecular biology, computational biology, genomics, structural biology, and microbiology with a particular focus on the structure and assembly of herpes simplex virus, human papilloma virus and Human gene expression. (wikipedia.org)
Brown's current research revolves around the control of human gene expression and Organization of transcription factor binding sites in the promoters of human genes. (wikipedia.org)
More recently, Brown has augmented his research expertise in analyzing gene expression. (wikipedia.org)
He compared the effect of promoters in genes strongly expressed in all tissues with genes that were expressed in a restricted set of tissues and identified the involvement of Polycomb Repressive Complex 2 genes of tissue-targeted transcription factor genes in the repression of transcription factor gene expression. (wikipedia.org)
Whilst working on gene expression, he proposed that for the protection of promoter's ability to regulate gene expression from the mutagenic damage the transcription binding sites of promoters are arrayed in multiple forms and ascertained supportive evidence in brain and liver-specific gene expression. (wikipedia.org)
R. Opavský, S. Pastoreková, V. Zelník, A. Gibadulinová, E. J. Stanbridge, J. Závada, R. Kettmann, J. Pastorek: Human MN/Ca9 gene, a novel member of the carbonic anhydrase family: Structure and exon to protein domain relationships. (learned.cz)
J. Závada, S. Pastoreková, J. Pastorek: MN Gene and Protein. (learned.cz)
Here, we report on the clinical impact of a novel NiV-derived nonspreading replicon particle lacking the fusion (F) protein gene (NiVF) as a vaccine in three small animal models of disease. (cdc.gov)

A child with smallpox in Bangladesh in 1973. (alquds.edu)

J. Závada: Pseudotypes of vesicular stomatitis virus with the coat of murine leukemia and of avian myeloblastosis viruses. (learned.cz)

Based on these criteria, he isolated two molecular weight classes of glycoproteins as the constituents of the plasma membrane of mouse L-929 cells and suggested that band 1 polypeptide might be efficiently involved in regulating L cell growth. (wikipedia.org)

He is a Professor Emeritus in the Department of microbiology, immunology, and cancer biology at the University of Virginia School of Medicine. (wikipedia.org)
After completing his post-doc in 1971, Brown joined the Department of Microbiology in the School of Medicine at the University of Virginia as Assistant Professor and ascended through the ranks to Professor in 1987. (wikipedia.org)

Aphtha is the Greek word for "vesicles in the mouth. (wikisource.org)
When added extracellularly, the non-protonated portion of chloroquine enters the cell, where it becomes protonated and concentrated in acidic, low-pH organelles, such as endosomes, Golgi vesicles, and lysosomes. (biomedcentral.com)

Vesicular diseases in swine are identical clinically. (wikisource.org)

Direct methods include quantifying virus salivated into op enhanced viremia and seroconversion compared with drops of blood, virus detected in vertebrate tissues imme- unbitten mice or to those co-injected with virus and mos- quito saliva ( 20 ). (cdc.gov)
decreases were seen in tissues and mucosal secretions, critically decreasing potential for virus transmission. (cdc.gov)

In addition, the indirect immunofluorescence assay described herein represents a simple and rapid method for screening SARS-CoV antiviral compounds. (biomedcentral.com)
In this report, we developed a novel neutralization assay for use in biosafety level 2 laboratories. (cdc.gov)

In his research, he has elucidated significant differences in the amounts of 4 glycopeptide classes extracted from vertebrate cells and also detected a glycopeptide which was only present during the cell-division period and named it glycol-peptide 4. (wikipedia.org)

Brown enrolled at Johns Hopkins University, in 1964, and graduated with a BSc in biology. (wikipedia.org)

He held appointments as a vice-chair in 1998 and a chair in 1999, under the cancer study section at the American Cancer Society. (wikipedia.org)

[25] [26] In 1967, the WHO intensified efforts to eliminate the disease. (alquds.edu)

Less virus was transmitted by mosquitoes in Since most saliva is expectorated during probing, saliva- vivo (geometric mean 11 PFU) than was found for compa- tion into hanging drops or capillary tubes may be inaccu- rable times of salivation in vitro (mean saliva titer 74 PFU). (cdc.gov)
We have recently shown that the processing of the spike protein was effected by furin-like convertases and that inhibition of this cleavage by a specific inhibitor abrogated cytopathicity and significantly reduced the virus titer of SARS-CoV [ 6 ]. (biomedcentral.com)

DHEA has been used safely for up to 2 years, usually in doses of 50 mg daily. (medlineplus.gov)
DHEA is possibly unsafe when used in high doses or long-term. (medlineplus.gov)
Do not use DHEA in doses higher than 50-100 mg daily or for a long period of time. (medlineplus.gov)

The amount of several arboviruses transmitted by mos- sents the low end of the in vivo transmission range. (cdc.gov)

Thereafter, he and his team began to work on the structural and functional properties of herpes simplex virus. (wikipedia.org)

These outbreaks are most often caused by Ebola virus, but at least three related viruses can also cause the disease. (cdc.gov)
Availability of a safe and efficacious vaccine is critical for restricting outbreaks and preventing disease in endemic countries. (cdc.gov)
[16] [17] The disease historically occurred in outbreaks . (alquds.edu)

1 fer in level or duration of viremia, clinical manifestations, in Florida ( 29 ) were reared at 27°C and a relative humidi- pathology, or antibody response ( 23 ). (cdc.gov)
J. Závada, Z. Závadová, S. Pastoreková, F. Čiampor, J. Pastorek, V. Zelník: Expression of MaTu-MN protein in human tumor cultures and in clinical specimens. (learned.cz)

Cytokine expression in the ney cells electroporated with transcribed RNA was used skin of mice infected with Sindbis virus differs after injec- for all experiments. (cdc.gov)

We previously developed a virus-like replicon particle (VRP) vaccine that provides complete protection against homologous and heterologous lethal CCHFV challenge in mice after a single dose. (cdc.gov)
This virus replicon particle system provides a vital tool to the field and demonstrates utility as a highly efficacious and safe vaccine candidate that can be administered parenterally or mucosally to protect against lethal Nipah disease. (cdc.gov)

J. Závada, L. Černý, Z. Závadová, J. Božoňová, A.D. Altstein: A rapid neutralization test for antibodies to bovine leukemia virus, with the use of rhabdovirus pseudotypes. (learned.cz)
NP antibodies are not associated with protection through neutralizing activity, but VRP vaccination results in NP antibodies possessing Fc-mediated antibody effector functions, such as complement activation (ADCD) and antibody-mediated cellular phagocytosis (ADCP). (cdc.gov)

Mice have higher seroconversion rates to *University of Texas Medical Branch, Galveston, Texas, USA vesicular stomatitis virus when infected by Ae . (cdc.gov)
Here we comprehensively characterized the kinetics of cell-mediated and humoral immune responses in VRP-vaccinated mice, and demonstrate that they predominantly target the nucleoprotein (NP). (cdc.gov)

We compared the amount of Venezuelan arbovirus transmission and concluded that allowing mos- equine encephalitis virus collected from the saliva of Aedes quitoes to feed on serum (similar to the capillary method) taeniorhynchus to the amount injected into a mouse during is less efficient in detecting virus than other methods. (cdc.gov)

In Italy in 1546, Fracastorius made what is probably the first report of a vesicular disease ( Bulloch 1927 ). (wikisource.org)
Severe acute respiratory syndrome (SARS) is an emerging disease that was first reported in Guangdong Province, China, in late 2002. (biomedcentral.com)
family Nairoviridae) is a tick-borne pathogen that frequently causes lethal disease in humans. (cdc.gov)
Nipah virus (NiV) causes a highly lethal disease in humans who present with acute respiratory or neurological signs. (cdc.gov)
Smallpox was an infectious disease caused by variola virus (often called smallpox virus) which belongs to the genus Orthopoxvirus . (alquds.edu)
The earliest evidence of the disease dates to around 1500 BC in Egyptian mummies . (alquds.edu)
[10] In 18th-century Europe, it is estimated that 400,000 people died from the disease per year, and that one-third of all cases of blindness were due to smallpox. (alquds.edu)
[10] Smallpox is one of two infectious diseases to have been eradicated, the other being rinderpest (a disease of even-toed ungulates ) in 2011. (alquds.edu)

Following the completion of his Ph.D., he received a post-doctoral NATO fellowship in molecular biology and joined the MRC Laboratory of Molecular Biology at Cambridge, where he worked under the supervision of Francis Crick. (wikipedia.org)
During my sabbatical leave I worked in the laboratory of Dr. Paul J. Davis, Chairman, Department of Medicine, Albany Medical College, Albany, New York. (sprayprotectgo.com)

He then undertook research in the area of Biochemistry and Molecular Biology at Harvard University, and earned his Ph.D. in 1969, under the mentorship of Paul M. Doty. (wikipedia.org)
Brown began his work in molecular biology with a substantial interest in the genetic code. (wikipedia.org)
During my stay I acquired certain skills in techniques dealing with molecular biology, such as, cDNA technology including Polymerase Chain Reaction (PCR) related to the research projects. (sprayprotectgo.com)

[22] [23] Europe adopted this practice from Asia in the first half of the 18th century. (alquds.edu)

However, demic Venezuelan equine encephalitis virus (VEEV) vec- amount of virus collected in vitro may not accurately tor, Aedes ( Ochlerotatus ) taeniorhynchus, salivates reflect mosquito transmission. (cdc.gov)

J. Závada, Z. Závadová, J. Pastorek, Z. Biesová, J. Ježek, J. Velek: Human tumor-associated cell adhesion protein MN/CA IX: Identification of M75 epitope and of the region mediating cell adhesion. (learned.cz)

A .gov website belongs to an official government organization in the United States. (medlineplus.gov)

[32] Subclinical ( asymptomatic ) infections with variola virus were noted but were not common. (alquds.edu)

DHEA levels seem to be lower in people with depression and after menopause. (medlineplus.gov)
The bumps filled with thick fluid and a depression or dimple in the center are characteristic. (alquds.edu)

Quantifying the dose of an arbovirus transmitted by diately after mosquito feeding, virus salivated into blood mosquitoes is essential for designing pathogenesis studies agar, and virus salivated into fluids such as oil. (cdc.gov)

when VEEV is passaged in cell culture ( 28 ). (cdc.gov)
Chloroquine is effective in preventing the spread of SARS CoV in cell culture. (biomedcentral.com)

These inhibitory effects are observed when the cells are treated with the drug either before or after exposure to the virus, suggesting both prophylactic and therapeutic advantage. (biomedcentral.com)

Taking DHEA by mouth or applying it to the skin might improve skin appearance after menopause and in people over the age of 60 years. (medlineplus.gov)
Taking DHEA by mouth before in-vitro fertilization (IVF) might improve the chances of pregnancy and having a baby. (medlineplus.gov)
Taking DHEA by mouth daily for up to 2 years doesn't seem to improve body shape, bone strength, muscle strength, or quality of life in people older than 60 who have low DHEA levels. (medlineplus.gov)
Taking DHEA by mouth doesn't improve muscle strength in adults. (medlineplus.gov)
Taking DHEA by mouth doesn't improve physical performance in older adults. (medlineplus.gov)
Taking DHEA by mouth does not seem to improve mental function or decrease mental decline in healthy older people, people with HIV, or in healthy young adults. (medlineplus.gov)
[5] This was followed by formation of ulcers in the mouth and a skin rash . (alquds.edu)

Nipah virus (NiV) is a highly pathogenic paramyxovirus with a high case fatality rate. (cdc.gov)

I was involved in several research projects, some were continuation of our collaboration which began almost a decade ago. (sprayprotectgo.com)
I also interacted in a positive manner with several faculty members, graduate and medical students in the Department of Medicine as well as researchers from the Wadsworth Center For Laboratories and Research, Albany, New York. (sprayprotectgo.com)

These viruses are small (22-30 nm), nonenveloped, and labile below pH 7.0, and they have single-stranded positivesense RNA with approximately 8450 nucleotides that serve as messenger RNA. (wikisource.org)

The importance of vesicular diseases in today's world-trade environment remains high. (wikisource.org)

The history and distribution of these viruses are given in Table 25.1. (wikisource.org)

DHEA can affect how insulin works in the body. (medlineplus.gov)
DHEA is a hormone that can affect how estrogen works in the body. (medlineplus.gov)

Anatomy15

Organisms51

Diseases10

Chemicals and Drugs43

Analytical, Diagnostic and Therapeutic Techniques and Equipment15

Phenomena and Processes33

Information Science4

Health Care1

Geographicals2

Glycoprotein2

19771

20181

Africa1

19342

Virology1

Viral1

Infection12

Widely1

20221

Recombinant2

Virion1

Gene8

19731

Coat1

Polypeptide1

Department of Microbiology2

Vesicles2

Clinically1

Tissues2

Assay2

Vertebrate1

19641

19991

19671

Titer2

Doses3

Vivo1

Structural1

Outbreaks3

Clinical2

Differs1

Particle2

Antibodies2

Mice2

Methods1

Disease8

Laboratory2

Molecular biology3

Europe1

Accurately1

Human1

Belongs1

Infections1

Depression2

Dose1

Cell culture2

Cells1

Mouth7

Case1

Research2

Small1

High1

Distribution1

Affect2