Proteins expressed sequence tags. Medical search. Frequent questions

We have catalogued the protein kinase complement of the human genome (the "kinome") using public and proprietary genomic, complementary DNA, and expressed sequence tag (EST) sequences. (nordiag.com)
The genomic organization of the human genes that encode protein prenyltransferases is shown in Figure 1 . (biomedcentral.com)
We identified zebrafish and Fugu TNFSF 13b homologues by genomic DNA database searches and determined that the predicted proteins share 68% and 76% sequence identity with trout TNFSF 13b. (usda.gov)
Three introns were identified in the rainbow trout, Atlantic salmon and zebrafish genomic sequences, and the intron locations correspond closely to those in the mammalian TNFSF 13b genes. (usda.gov)
Using information from most sequenced eukaryotic lineages, our phylogenetic analysis is based on full-length cDNA-, EST-derived-and genomic- PDZ and LIM domain sequences of over 25 species, ranging from yeast to humans. (princeton.edu)
Stanke M, Tzvetkova A, Morgenstern B: AUGUSTUS at EGASP: using EST, protein and genomic alignments for improved gene prediction in the human genome. (crossref.org)
Therefore comparative genomic analysis of T . equi was undertaken to: 1) identify genes contributing to immune evasion and persistence in equid hosts, 2) identify genes involved in PBMC infection biology and 3) define the phylogenetic position of T . equi relative to sequenced apicomplexan parasites. (biomedcentral.com)
Comparative genomic analysis of T . equi revealed the phylogenetic positioning relative to seven apicomplexan parasites using deduced amino acid sequences from 150 genes placed it as a sister taxon to Theileria spp . (biomedcentral.com)
Advances in genomic sequencing technology are transforming medical discovery, enabling researchers to conduct comprehensive explorations across whole genomes for individuals and populations. (cdc.gov)
Array comparative genomic hybridization (aCGH) is a type of microarray now routinely used to identify deleted or duplicated regions of DNA sequence in specific chromosomes on a genome-wide basis. (msdmanuals.com)
He also noted concern regarding delays in referring specimens to the laboratory for testing, and emphasized the need for improvement in timeliness of reporting and tracking of the viruses referred for genomic sequencing. (who.int)
He thanked the regional and global specialized laboratories for their efforts to provide timely results of the genomic sequencing of wild polioviruses, and for their help in planning and monitoring polio eradication activities in the Region. (who.int)

We have identified four members of this subfamily in Arabidopsis thaliana, one of them with associated expressed sequence tags (ESTs). (usu.edu)
As a first step in pursuing this research, we report the generation and analysis of expressed sequence tags (ESTs) from the male accessory gland of H. erato and H. melpomene, species representative of the two mating systems present in the genus Heliconius. (ku.edu)
Results: We successfully sequenced 933 ESTs clustering into 371 unigenes from H. erato and 1033 ESTs clustering into 340 unigenes from H. melpomene. (ku.edu)
Conclusion: This collection of ESTs will serve as the foundation for the future identification and evolutionary analysis of male reproductive proteins in Heliconius butterflies. (ku.edu)
Automatic comparisons of data from expressed sequence tags (ESTs) with genes (for example using the program Acembly, for which the results are available from the NCBI AceView server [ 2 ]) shows that all the human protein prenyltransferase genes have multiple alternative splice variants. (biomedcentral.com)
Sequencing of 16 M. persicae cDNA libraries generated 26,669 expressed sequence tags (ESTs). (nih.gov)
It has been estimated that ~100,000 expressed sequence tags ( ESTs ) can be identified in humans. (wikidoc.org)
Nearly 39% of the ESTs were predicted to encode proteins of fungal origin and among these, 162 ESTs were predicted to contain N-terminal signal peptides (SPs) in their deduced open reading frames (ORFs). (biomedcentral.com)

Here, we used a genomics/proteomics approach (including immunoblot experiments from pigs infected with T. suis ) to prioritize putative immunogenic excretory/secretory (E/S) proteins conserved across and specific to several gastrointestinal (GI) parasitic nematode species. (frontiersin.org)
Biochimica et Biophysica Acta - Proteins and Proteomics. (illinois.edu)
New insights into the genome have led to the emergence of proteomics , the study of the structure and function of an individual's entire set of expressed proteins. (cdc.gov)
Proteomics is highly linked to genomics, since the blueprint for each protein is inscribed on an organism's genes. (cdc.gov)
Some challenges for translating proteomics include technical issues in measuring proteomic markers, identifying clinically useful markers in patient samples, the need for large sample sizes to be able to distinguish protein variability among samples, and challenges in implementing strict protocols for sample handling. (cdc.gov)

High-density oligonucleotide arrays were used to identify genes differentially expressed between high and low ERG expressers. (nih.gov)
One-third of the 471 genes predicted to encode transcription factors are differentially expressed during sexual development of S. commune . (nature.com)
Quantitative PCR and high-throughput methods, such as expressed sequence tag analysis, have been used for measuring gene expression and identifying subsets of elm genes that are differentially expressed in the presence of O. novo-ulmi . (sisef.it)
These analyses have also helped identify genes that were differentially expressed in DED fungi grown under defined experimental conditions. (sisef.it)

An antibody-protein array of putative immunogenic proteins was developed from a combined bioinformatic, experimental, and literature-based prioritization of homologous parasite proteins. (frontiersin.org)
The GPCR97 clone was used to probe a human thalamus library, which resulted in the isolation of a full-length clone encoding a putative G protein-coupled receptor. (aspetjournals.org)
Eleven candidate effector genes were identified based on features common to characterized fungal effectors, i.e. they encode small, soluble (lack of transmembrane domain), cysteine-rich proteins with a putative SP. (biomedcentral.com)
Each sequence from this database was used to search public protein databases to assign putative function to as many database entries as possible. (usda.gov)

Over the last two decades, several laboratories have used a wide range of techniques encompassing expressed sequence tag (EST) analysis, serial analysis of gene expression (SAGE), microarray and next-generation sequencing (NGS) technologies for analysing the chickpea transcriptomes. (intechopen.com)
These include previously unknown sequence data, a collection of expressed genes, molecular markers, and a DNA microarray that can be used to study aphid gene expression. (nih.gov)

Fascin-2 is a protein that in humans is encoded by the FSCN2 gene. (wikipedia.org)
This gene encodes a member of the fascin protein family. (wikipedia.org)
Xpro is a relational database that contains all the eukaryotic protein-encoding DNA sequences contained in GenBank with associated data required for the analysis of eukaryotic gene architecture. (edu.au)
Hansell Stedman and collaborators at the University of Pennsylvania had collected sequence data for a gene called MYH16 . (johnhawks.net)
Other primates express this gene in their jaw muscles, where it strengthens the bite force. (johnhawks.net)
They looked at more than 1100 base pairs of sequence from a chimpanzee, orangutan, macaque, and a dog, and they found that the coding sequence in humans has a small number of unexpected amino-acid-coding changes-changes that wouldn't be likely if the gene product was still being maintained by selection. (johnhawks.net)
Gene structures and chromosomal locations of human protein prenyltransferase subunit genes. (biomedcentral.com)
Among the known genes uncovered in the screen were those encoding proteins that function in cell division, cell signalling and gene/protein expression. (elsevierpure.com)
Northern blot analysis documented that two transcription factor genes chosen for further study, c-myc promoter-binding protein (MBP-1) and X-box binding protein 1 (XBP-1), were up-regulated in U266 cells about 3-fold relative to the cell cycle-dependent β-actin gene 12 h after IL-6 treatment. (elsevierpure.com)
Until recently, however, functional analysis of the DED fungi was hampered by the lack of protocols for efficient gene knockout and by the unavailability of a full genome sequence. (sisef.it)
Gelfand M, Mironov A, Pevzner P: Gene recognition via spliced sequence alignment. (crossref.org)
Single-gene knockout-coupled omics analysis identifies C9orf85 and CXorf38 as two uncharacterized human proteins associated with ZIP8 malfunction. (nih.gov)
In order to clarify the biological and pharmacological mechanisms of O. sinensis , we carried out the genome sequencing of H. sinensis, anamorph of O. sinensis , for the first time, described the annotation and gene expression, and analyzed the complete sequencing data (Li et al. (springeropen.com)
Protein A, B and C are isoforms encoded from the same gene through alternative splicing . (wikidoc.org)
Different forms of a protein may be produced from different but related genes , or may arise from the same gene by alternative splicing . (wikidoc.org)
the ability to create catalytically different proteins from the same gene increases the diversity of the proteome. (wikidoc.org)
A protein isoform, or "protein variant", [1] is a member of a set of highly similar proteins that originate from a single gene or gene family and are the result of genetic differences. (wikidoc.org)
a "different sequence of a gene (locus)" [6] is called a variant . (wikidoc.org)
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. (cancerindex.org)
What does this gene/protein do? (cancerindex.org)
Sequence homology is an indication of the genetic relatedness of different organisms and gene function. (lookformedical.com)
Nucleotide sequences, usually upstream, which are recognized by specific regulatory transcription factors, thereby causing gene response to various regulatory agents. (lookformedical.com)
The life cycle of a protein starts with a blueprint transcribed from a gene, followed by folding, which translates the blueprint to a three-dimensional structure that allows it to carry out its biological function, and ends with degradation. (cdc.gov)
Overview of Genetics A gene, the basic unit of heredity, is a segment of DNA containing all the information necessary to synthesize a polypeptide (protein) or a functional RNA molecule. (msdmanuals.com)

Among these tick salivary proteins are lipocalins, antiparallel beta-barrel proteins that sequester small molecules. (biomedcentral.com)

In both species only a third of accessory gland unigenes were also found among genes expressed in wing tissue. (ku.edu)
About 25% of unigenes from both species encoded secreted proteins. (ku.edu)
In addition, post-translational modifications can also contribute to the increased complexity and diversity of protein species [ 3 ]. (biomedcentral.com)

Monoclonal mouse Anti·His Antibodies (5xHis antibodies and 4xHis antibodies), like the RGS·His Antibody, BSA-free, Tetra·His Antibody, BSA-free and Penta·His Antibody, BSA-free are used to detect recombinant protein carrying His tags. (qiagen.com)
Recombinant protein rAra h 2 is expressed in Escherichia coli. (exbio.cz)
A calculated molecular mass of recombinant protein is 19,5 kDa. (exbio.cz)
Recombinant protein rAra h 2 - 1 vial (0.1 mg), lyophilized. (exbio.cz)

McAuliffe J, Pachter L, Jordan M: Multiple-sequence functional annotation and the generalized hidden Markov phylogeny. (crossref.org)

Three different protein prenyltransferases (farnesyltransferase and geranylgeranyltransferases I and II) catalyze the attachment of prenyl lipid anchors 15 or 20 carbons long to the carboxyl termini of a variety of eukaryotic proteins. (biomedcentral.com)
The chromosomal locations and number of exons from protein prenyltransferase genes in the major eukaryotic model organisms are shown in Table 2 . (biomedcentral.com)

Genes contributing to the similarity include ribosomal protein (RP) genes as well as genes involved in transcription, translation and cell division. (karger.com)
Of these, 29 were found to correspond to known genes, 22 matched expressed sequence tags in public databases and 21 showed no sequence similarity to any existing entries. (elsevierpure.com)

This thematic series of companion papers constitutes the initial exploration of the genome biology of the waterflea (Daphnia) building on the first complete crustacean genome sequence. (biomedcentral.com)
10,200 protein-encoding genes were predicted based on the genome sequence. (springeropen.com)
The genes and proteins were predicted and annotated based on the genome sequence. (springeropen.com)

Recent studies suggest that most of the genome are transcribed, among the transcripts only a small portion encode for proteins, whereas a large portion of the transcripts do not encode any proteins, which are generally termed non-protein coding RNAs (npcRNA). (biomedcentral.com)

For detection, QIA express mouse monoclonal antibodies are produced under conditions that guarantee the highest purity and activity. (qiagen.com)

In addition to the information found in the GenBank records, which includes properties such as sequence, position, length and description about introns, exons and protein-coding regions, Xpro provides annotations on the splice sites and intron phases. (edu.au)
Sequences of DNA in the genes that are located between the EXONS. (lookformedical.com)

Sensitivity may vary with different proteins. (qiagen.com)

As with other mitochondrial carrier family proteins, the ornithine carrier is composed of 300 amino acids that constitute 3 repeated motifs of approximately 100 amino acids each. (medscape.com)
The transporter was identified by probing a mammalian-expressed sequence tag database with 2 fungal mitochondrial ornithine carrier protein sequences. (medscape.com)
Mitochondrial and chloroplast proteins are therefore synthesized in the cytosol as preproteins with an NH 2 -terminal targeting sequence, which is both necessary and sufficient for directing the preproteins to the correct organelle. (silverchair.com)

The precursor forms of prominent chloroplast proteins like the small subunit of stromal Rubisco (SSU), the thylakoid a/b binding protein (LHCP), or the thylakoid lumen localized subunits of the oxygen evolving complex of 23 kD and 33 kD (OE23, OE33) contain a phosphorylation site within the chloroplast targeting signal ( Waegemann and Soll 1996 ). (silverchair.com)
In the Fp subunit of SQR, and the closely related membrane-associated fumarate reductase of anaerobic organisms, FAD is covalently attached via an 8α-N(3)-histidyl linkage to a histidine (His) in a conserved sequence in the N-terminal part of the polypeptide. (lu.se)

By comparing the sequences of humans and other primates, Stedman's team estimated that MYH16 had lost its function in human ancestors around 2.4 million years ago. (johnhawks.net)
There are three different protein prenyltransferases in humans: farnesyltransferase (FT) and geranylgeranyltransferase 1 (GGT1) share the same motif (the CaaX box) around the cysteine in their substrates, and are thus called CaaX prenyltransferases, whereas geranylgeranyltransferase 2 (GGT2, also called Rab geranylgeranyltransferase) recognizes a different motif and is thus called a non-CaaX prenyltransferase [ 1 ]. (biomedcentral.com)
The Alu sequence family (named for the restriction endonuclease cleavage enzyme Alu I) is the most highly repeated interspersed repeat element in humans (over a million copies). (lookformedical.com)

Transcriptome analysis has facilitated the development of molecular markers such as simple sequence repeats (SSRs), single-nucleotide polymorphisms (SNPs) and potential intron polymorphisms (PIPs) that are being used to expedite the chickpea breeding programmes. (intechopen.com)
As we recently reported, analysis of 77 FHWT by whole genome sequencing (WGS, 58 patients) or whole exomic sequencing (WES,19 patients) identified 825 high-quality somatic, non-synonymous variants, with an average of 11 candidate mutations/case (range 2-42) (ref. 6 ). (nature.com)
Sequence analysis of the Daphnia pulex genome holds some surprises that could not have been anticipated from what was learned so far from other arthropod genomes. (biomedcentral.com)
To define the molecular basis of the IL-6-induced mitogenic response in myeloma cells, we applied STAR (subtractive transcriptional amplification of mRNA), a new differential expression analysis technology, to isolate mRNAs preferentially expressed in IL-6-treated versus untreated cultures of the factor-responsive myeloma cell line U266. (elsevierpure.com)
Durbin R, Eddy S, Krogh A, Mitchison G: Biological sequence analysis: Probabilistic models of proteins and nucleic acids. (crossref.org)
DNA sequence and analysis of human chromosome 9. (nih.gov)

Concomitant activation of the insulin-like growth factor-1 receptor, Akt, and extracellular signal-regulated protein kinase 1 and 2 as well as increased phosphorylation of Bad also were unique to the hippocampus of APP Sw mice. (jneurosci.org)
The protein kinase complement of the human genome. (nordiag.com)
The protein kinase is found in the cytosol of pea mesophyll cells or in wheat germ extract. (silverchair.com)

Sequences were assembled into a database of assembled contigs and singletons and used to search public protein databases and annotate the sequences. (usda.gov)

Furthermore, Xpro validates intron positions using alignment information between the record's sequence and EST sequences found in dbEST. (edu.au)

Using potato virus X (PVX) based transient expression assays, we showed that one of the candidate effectors, i. e. contig 8 that encodes a cerato-platanin (CP) domain containing protein, unlike CP proteins from other fungal pathogens was unable to elicit a hypersensitive response (HR). (biomedcentral.com)

The genes encoding CaaX protein prenyltransferases are considerably longer than those encoding non-CaaX subunits, as a result of longer introns. (biomedcentral.com)

High ERG expression was associated with upregulation of 112 expressed-sequenced tags and named genes, many of which are involved in cell proliferation, differentiation, and apoptosis. (nih.gov)

S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. (cancerindex.org)
If the membrane-anchor is missing or defective, Fp and Ip subunits accumulate as soluble proteins in the cytoplasm. (lu.se)

Comparison of cDNA data from three aphid lineages identified single nucleotide polymorphisms that can be used as genetic markers and, in some cases, may represent functional differences in the protein products. (nih.gov)
any "of several different forms of the same protein, arising from either single nucleotide polymorphisms, [4] differential splicing of mRNA, or post-translational modifications (e.g. sulfation, glycosylation, etc.)" [5] is called an isoform , or protein isoform . (wikidoc.org)

The 162 sequences could be assembled into 122 tentative unigenes comprising 32 contigs and 90 singletons. (biomedcentral.com)
Sequence analyses of unigenes revealed four potential groups: hydrolases, cell envelope associated proteins (CEAPs), candidate effectors and other proteins. (biomedcentral.com)

Tg2576 mice overexpress a mutant form of human amyloid precursor protein with the Swedish mutation (APP Sw ), resulting in high β-amyloid (Aβ) levels in the brain. (jneurosci.org)
Briefly, they contain the human APP 695 with the double mutation K670N and M671L (Swedish mutation) and are driven by the prion protein promoter. (jneurosci.org)
Although a recent study ( 22 ) reported enhanced avidity of H5N6 viruses for human-type receptors, recombinant clade 2.3.4.4 highly pathogenic influenza A virus H5 proteins from virus isolates in North America show a strict avian receptor-binding preference ( 23 ). (cdc.gov)
The human coding sequence of MYH16 has a frameshift mutation that prevents translation of the functional product. (johnhawks.net)
Recent advances in sequencing technology make it possible to comprehensively catalog genetic variation in population samples, creating a foundation for understanding human disease, ancestry and evolution. (nordiag.com)
Bortoluzzi S, Alessi FD, Romualdi C, Danieli GA: Differential expression of genes coding for ribosomal proteins in different human tissues. (karger.com)
The trout TNFSF 13b predicted protein shares 54% amino acid identity with human and chicken TNFSF 13b, and 51% identity with mouse TNFSF 13b. (usda.gov)
Biotin labeled cRNA transcripts were synthesized, fragmented and hybridized to HuGeneFL GeneChip probe arrays representing more than 6800 human genes and expressed sequence tags. (cdc.gov)
Aided by state-of-the-art, high-throughput computational methods, the current study simulated and analyzed the dynamics of competitive histamine binding at the tick-host interface using the available crystal structures of both the tick salivary lipocalin histamine-binding protein from Rhipicephalus appendiculatus and the human histamine receptor 1. (biomedcentral.com)
Similarly, the number of protein-coding genes in human and nematode is extremely close. (biomedcentral.com)
DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom. (lookformedical.com)
Sera from residual specimens from NHANES 2001-2002 participants were tested for levels of IgG antibodies against cancer antigen (CA) 125, CA15.3 and human epididymal secretory protein 4 (HE4). (cdc.gov)
Studies of the human proteome have enabled scientists to track protein synthesis, modification, and degradation over time and across different cell types. (cdc.gov)
In 2014, scientists developed a draft map of the human proteome , which catalogued proteins encoded by over 17,000 human genes, or about 84% of all protein-coding genes in the human genome. (cdc.gov)
The "self"-protein albumin in mouse airway fluid was uniquely modified by GSH-MDI at position 414K, a preferred site of MDI reactivity on human albumin. (cdc.gov)

Anti·His Antibodies enable identification of positive expression clones by colony blotting (see figure Identification of positive expression clones by colony blotting. . "> "Identification of positive expression clones by colony blotting" ) and detection of His-tagged thioredoxin (see figure His-tagged thioredoxin detected by Penta·His Antibody in yeast cells. . "> "His-tagged thioredoxin detected by Penta·His Antibody in yeast cells" ). (qiagen.com)
Streamlining Regulon Identification in Bacteria Regulons are a group of genes that can be turned on or off by the same regulatory protein. (doe.gov)
Identification of intrahepatic cholangiocarcinoma related genes by comparison with normal liver tissues using expressed sequence tags. (nih.gov)

Screening the array with sera and ileal fluid samples from immunized pigs suggested cross-reactivity among homologous proteins and a general activation of immunity. (frontiersin.org)

To gain deeper insight into the biological and pharmacological mechanisms, we sequenced the genome of H. sinensis . (springeropen.com)
Exhibiting high consistence in sequence and structure, S100 family members are interchangeable in function and they show a wide spectrum of biological processes, including proliferation, apoptosis, migration, inflammation and differentiation and the like. (cancerindex.org)

2014). Where annotations were not present or were different, the online Softberry server (http://linux1.softberry.com/berry.phtml) was used to predict mRNA and protein sequences using the FGNESH+ prediction algorithm (Solovyev et al. (molquest.com)

We describe a new subfamily of WD repeat proteins characterised by the presence of a C3H zinc finger at the N-terminal part of the protein associated with seven WD40 repeats. (usu.edu)

Trout TNFSF 13b RNA is expressed in immune tissues including the spleen, anterior kidney, and peripheral blood leukocytes but not in the liver. (usda.gov)

The α subunits of protein prenyltransferases consist of tetratricopeptide repeats and are part of the tetratricopeptide repeat superfamily [ 5 ], which also includes functionally diverse proteins involved in transcription, co-chaperoning, protein transport, cell-cycle control and phosphorylation. (biomedcentral.com)
The region of DNA which borders the 3' end of a transcription unit and where a variety of regulatory sequences are located. (lookformedical.com)

As conventional alignment and phylogenetic procedures of full-length sequences fell short of elucidating the evolutionary history of these genes, we started to analyze the PDZ and LIM domain sequences themselves. (princeton.edu)

From the resulting collection of STAR clones, sequence information was obtained for a total of 72 distinct transcripts. (elsevierpure.com)
Approximately 5,400 clones from each library were sequenced from both the 5' and 3' directions. (usda.gov)

We have initiated study of the genes of the New World Screwworm, Cochliomyia hominivorax, by synthesizing and sequencing DNA libraries of expressed genes from embryos, 2nd instar larvae, adult males and adult females. (usda.gov)

Our data suggest an important role for these proteins. (usu.edu)
A framework for variation discovery and genotyping using next-generation DNA sequencing data. (nordiag.com)
A Better Way to Find RNA Virus Needles in the Proverbial Database Haystacks Researchers combed through more than 5,000 data sets of RNA sequences generated from diverse environmental samples around the world, resulting in a five-fold increase of RNA virus diversity. (doe.gov)
Three explanatory types of data can be obtained from the current study: (i) the dynamics of multiple binding sites, (ii) competition between two proteins for a ligand, and (iii) the intrinsic molecular components involved in the competition. (biomedcentral.com)

These motifs contain 2 hydrophobic alpha-helical segments connected by an extensive hydrophilic sequence, resulting in 6 transmembrane portions of the protein. (medscape.com)

Extensive analyses of transcriptome have been carried out in chickpea, which is the third most important legume valued as a source of dietary protein and micronutrients. (intechopen.com)

HA proteins bind to sialoside receptors on the host cell surface. (cdc.gov)

The PDZ and LIM domain-containing protein family is encoded by a diverse group of genes whose phylogeny has currently not been analyzed. (princeton.edu)

epithelial cells of the small intestine and kidney also express these enzymes to a lesser extent, but their contribution to urea production is not significant. (medscape.com)
Tag ® sequence and expressed in NSO cells. (merckmillipore.com)
By cataloguing the molecular processes by which cells maintain and modify protein levels, proteomic studies offer another dimension of information that may help advance our understanding of health and disease. (cdc.gov)

The tagged DNA binds to its complementary DNA segment and can be detected by measuring the amount and type of fluorescence. (msdmanuals.com)

This technology involves breaking the entire genome into small segments, sequencing the segments, and then reassembling the sequences using intensive computational techniques to provide the base-by-base sequence of the entire genome or more limited regions, such as the expressed portion of the genome known as the exome. (msdmanuals.com)

However, the sheer volume of information generated by sequencing the exome or genome results in a variety of interpretive problems that complicate understanding of the results. (msdmanuals.com)

Each of the three variants detects a different His tag epitope. (qiagen.com)
Each mutation was verified by Sanger sequencing of PCR products spanning the variants ( Fig. 1 ). (nature.com)

This study aimed to evaluate the genetic variability of the teak germplasm bank, using morphological traits and inter-simple sequence repeat molecular markers. (geneticsmr.com)

A cocktail of five recombinant proteins optimized for conserved GI nematode targets was used immunize pigs and test for active antibody responses in both the serum and intestinal ileal fluid of immunized pigs. (frontiersin.org)

The QIA express System provides materials for the expression, purification, detection and assay of 6xHis-tagged proteins. (qiagen.com)
The expression of transthyretin, a protein shown to sequester Aβ and prevent amyloid fibril formation in vitro , and several genes in the insulin-signaling pathway, e.g., insulin-like growth factor-2, were increased selectively in the hippocampus of APP Sw mice. (jneurosci.org)
Similarly, expression of heat shock protein 70 increased 8.8 fold in TCA treatment group compared to TCE treatment which had 2.7 fold increase. (cdc.gov)
Scope includes mutations and abnormal protein expression. (cancerindex.org)

Simple sequence repeats (SSRs) are highly variable features of all genomes. (biomedcentral.com)

Proteins that activated immunity are potential antigens for immunization and the multi-omics phylum-spanning prioritization database that was created is a valuable resource for identifying target proteins in a wide array of different parasitic nematodes. (frontiersin.org)

Anatomy9

Organisms15

Chemicals and Drugs13

Analytical, Diagnostic and Therapeutic Techniques and Equipment18

Phenomena and Processes42

Disciplines and Occupations3

Technology, Industry, Agriculture4

Information Science12

Health Care1

Genomic12

ESTs8

Proteomics5

Differentially expressed4

Putative4

Microarray2

Gene24

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Species3

Recombinant protein4

Annotation1

Eukaryotic2

Similarity2

Genome sequence3

Encode1

Mouse monoclonal1

Exons2

Different proteins1

Mitochondrial3

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Humans3

Analysis6

Kinase3

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Intron1

Fungal1

Introns1

Cell Proliferation1

Cytoplasm2

Single-nucleotide po2

Unigenes2

Human15

Identification3

Homologous1

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Tissues1

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