Protein protein capsid proteins. Medical search. Frequent questions

Partial sequencing of the B646L gene encoding the major capsid protein p72 has so far led to the identification of 22 ASFV genotypes. (cdc.gov)
Nucleotide sequences encoding the major capsid protein VP1 were determined for each isolate. (cdc.gov)

Using atomic force microscopy imaging and nanoindentation measurements, we investigated the effect of the minor capsid proteins pUL17 and pUL25 on the structural stability of icosahedral herpes simplex virus capsids. (vu.nl)
CroV is covered by a protein shell (capsid) comprised of major capsid proteins (MCP) and minor capsid proteins (mCP). (utep.edu)
Plug-like density features were observed at the base of the VP1 pentamers, consistent with the known location of minor capsid proteins VP2 and VP3. (rcsb.org)

The extra sequences at the N-termini of viral jelly roll capsid proteins, involved in recognizing the viral genome, likely evolved after the capture of these proteins from cells. (virology.ws)
The RSV capsid enclosing the viral genome materials is assembled from 1500 copies of the 237-residue RSV capsid protein CA. In vitro, tubular assembly can be obtained with the CA with similar underlying structural properties as the authentic RSV capsid. (dtic.mil)
This indicates that herpesviruses withstand the internal pressure that is generated during DNA genome packaging by locally reinforcing the mechanical sturdiness of the vertices, the most stressed part of the capsids. (vu.nl)
Sequences were obtained from 4 genome regions, including part of the gene B646L that encodes the p72 capsid protein, the complete E183L and CP204L genes, which encode the p54 and p30 proteins and the variable region of the B602L gene. (cdc.gov)
E proteins made by the viral genome promote the activation of host DNA replication mechanisms that can then be used by the virus during its own replication. (medscape.com)
How can this protein shell be strong enough to remain intact as it enters a host cell, and yet quickly open up to release the viral genome after replication? (elifesciences.org)
In Nature the genome of viruses are protected by protein capsids. (lu.se)
The capsid serves two purposes: to protect the genome from the outside and to trick cel s into incorporating the virus. (lu.se)
Viral capsids are protein coats found inside viruses that contain and protect the viral genome. (lu.se)

Chakroborty, Sayan, "Expression and characterization of major capsid protein (MCP) of a giant marine virus: Cafeteria roenbergensis virus (CroV)" (2013). (utep.edu)

Structure of the HIV-1 full-length capsid protein in a conformationally trapped unassembled state induced by small-molecule binding. (nature.com)
have now developed a method that can visualize individual HIV capsids - and how they disassemble - in real time using single-molecule microscopy. (elifesciences.org)
also found that a small molecule drug called PF74 causes the capsid to crack open rapidly, but the remaining shell is then stabilized against further disassembly. (elifesciences.org)
which identifies a molecule found in host cells that stabilizes the capsid of HIV. (elifesciences.org)

Retroviral structural proteins also appear to have originated from cell proteins, with clear homologies with matrix, capsid, and nucleocapsid proteins. (virology.ws)

SCOP: Structural Classification of Proteins and ASTRAL. (berkeley.edu)
We have previously discussed the idea that viruses originated from selfish genetic elements such as plasmids and transposons when these nucleic acids acquired structural proteins (see A plasmid on the road to becoming a virus ). (virology.ws)
I want to explore in more detail the idea that the structural proteins of viruses likely originated from cell proteins ( link to paper ). (virology.ws)
Missing from these hypothesis is how nucleic acids became virus particles - that is, how they acquired structural proteins. (virology.ws)
It seems likely that viral structural proteins originated from cellular genes. (virology.ws)
The core proteins of alphaviruses (think Semliki Forest virus) has structural similarity with chymotrypsin-like serine proteases. (virology.ws)
At some point these genetic elements acquired structural proteins from the cells and became bona fide virus particles. (virology.ws)
ELP-CP, a structural fusion protein of the thermally responsive elastin-like polypeptide (ELP) and a viral capsid protein (CP), was designed, and its assembly properties were investigated. (psu.edu)
This work shows the feasibility of creating a self-assembly system with new properties by combining two structural protein elements. (psu.edu)
Comparisons between the membrane-bound form of the coat protein and the previously determined structural form found in filamentous bacteriophage particles demonstrate that it undergoes a significant structural rearrangement during the membrane-mediated virus assembly process. (rcsb.org)
The N-terminal helix and the hinge that connects it to the transmembrane helix are significantly more dynamic than the rest of the protein, thus facilitating structural rearrangement during bacteriophage assembly. (rcsb.org)
however, structural information regarding the assembled capsid and the contacts between the assembly units is incomplete. (nature.com)
Cryo-EM structural data have been deposited in the EMDataBank under accession codesEMD-5582 andEMD-5639, and the MDFF atomic model of the CA HOH and models of HIV-1 capsid have been deposited in the Protein Data Bank under accession numbers 3J34 , 3J3Q , 3J3Y . (nature.com)
In this thesis project you wil work on characterizing a synthetic variant of a capsid protein from a natural virus that has been designed using computational structural modeling. (lu.se)
By explicitly modelling the shapes of the subunits in the cage and matching the shapes with proteins from structural databases, we find that we can create structures with many different sizes, shapes, and porosities - including low porosities. (lu.se)

The deduced amino acid sequences of the outer capsid protein, VP7, of serotype G1 rotavirus clinical isolates collected over a 6 year period (1990-1995) in Melbourne, Australia, were examined. (microbiologyresearch.org)
Phylogenetic analysis characterized the sequences into two discrete clusters representing two of the four global lineages of human G1 VP7 proteins. (microbiologyresearch.org)
The chip contains the sequences of 15 different capsid proteins converted into more than 5,000 overlapping peptides for high-resolution epitope data. (pepperprint.com)
Protein sequences were clustered by the pairwise sequence similarity using CLANS. (biomedcentral.com)

An analysis of the sequence an structure of major virion proteins has identified likely ancestors in cellular proteins. (virology.ws)
A protein-nucleic acid complex which forms part or all of a virion. (bvsalud.org)

Fourth-generation HIV immunoassays detect viral p24 protein in the blood (as well as patient antibodies against the virus). (wikipedia.org)
Available with linear or cyclic constrained peptides for linear or conformational epitope mapping, the PEPperCHIP ® Pan-AAV Capsid Protein Microarray can be used to analyze patient and animal sera as well as research and diagnostic anti-AAV antibodies on the epitope level. (pepperprint.com)
The immune system responds to antigens by producing cells that directly attack the pathogen, or by producing special proteins called antibodies . (khanacademy.org)

The matrix Z proteins of arenaviruses are related to cellular RING domain proteins, and the matrix proteins of some negative strand RNA viruses are related to cellular cyclophilin. (virology.ws)
There are many more examples, providing support for the hypothesis that viruses evolved on multiple instances by recruiting different cell proteins. (virology.ws)
Given this information on the origin of viral capsid proteins, we can modify the three hypotheses for the origin of viruses into one. (virology.ws)
Now, the question if of course - is it of viral origin or is it a cellular protein co-opted by viruses? (virology.ws)
WB/ELISA positive controls from Made for Virology provide stable, validated protein standards for a wide range of human viruses including respiratory tract pathogens, as well as, animal and marine pathogens. (nanoexpo.eu)
For some negative-strand RNA viruses (e.g., vesicular stomatitis virus and Ebola virus), the viral matrix (M) protein contains all of the information needed for budding, since virus-like particles (VLPs) are efficiently released from cells when the M protein is expressed from cDNA. (nih.gov)
Unlike the capsids of many other viruses, those of HIV have irregular structures that rapidly fall apart once removed from the virus. (elifesciences.org)
Some viruses have an outer envelope consisting of protein and lipid, surrounding a protein capsid complex with genomic RNA or DNA and sometimes enzymes needed for the first steps of viral replication. (msdmanuals.com)
In spherical viruses capsid proteins forms icosahedral shel s with hundreds of subunits. (lu.se)
The shel s of many viruses are made up by only a single type of subunit and can often form from a solution of purified protein subunits (they self-assemble). (lu.se)

The involvement of viral DNA-binding proteins in the regulation of virulence genes, transcription, DNA replication, and repair make them significant targets. (mdpi.com)
L genes encode viral capsid proteins. (medscape.com)
Disruption of E1 and E2 allows for dysregulated downstream genes and the expression of E6 and E7 proteins, which are selectively maintained in virally induced tumors. (medscape.com)

In this project we wil rebuild one of these amazing protein assemblies, spherical virus capsids, to function as nanoscale chemical reactors and containers for gene delivery of proteins into human cel s. (lu.se)
Protein assemblies are some of the most complex molecular machines in nature. (lu.se)
In a series of 3 papers, we analyzed the structure, developed structure prediction tools, and design tools, for different protein assemblies. (lu.se)
In another paper, we developed methods to predict large cubic symmetrical protein assemblies, such as viral capsids, from sequence. (lu.se)

pUL17 and pUL25, which form the capsid vertex-specific component (CVSC), particularly contributed to capsid resilience along the 5-fold and 2-fold but not along the 3-fold icosahedral axes. (vu.nl)

One example is syncytin , a retroviral protein used for the construction of the mammalian placenta. (virology.ws)

However, lowering charge beyond a certain point resulted in capsid aggregation and precipitation. (uky.edu)

A very common motif among viral capsid proteins is called the single jelly roll , made up of eight beta strands in two four-stranded sheets. (virology.ws)

It was found that M protein in the form of VLPs was only secreted from cells, with an efficiency comparable to authentic virus budding, when M protein was coexpressed with one of the two glycoproteins, HN or F, together with the nucleocapsid (NP) protein. (nih.gov)
To understand the intrinsic capsid disassembly pathway and how it can be modulated, we have developed a single-particle fluorescence microscopy method to follow the real-time uncoating kinetics of authentic HIV capsids in vitro immediately after permeabilizing the viral membrane. (elifesciences.org)
Detection of norovirus capsid protein in authentic standards and in stool extracts by matrix-assisted laser desorption ionization and nanospray mass spectrometry. (cdc.gov)

Many cell proteins have jelly role motifs, and some form 60-subunit virus-like particles in cells. (virology.ws)
Interestingly, this protein-based block copolymer could be self-assembled via two mechanisms into two different, well-defined nanocapsules: (1) pH-induced assembly yielded 28 nm virus-like particles, and (2) ELP-induced assembly yielded 18 nm virus-like particles. (psu.edu)
Conversely, truncation of the F protein cytoplasmic tail was not inhibitory and did not affect the ability of coexpressed wt HN protein to direct the budding of particles. (nih.gov)
Virus particles and purified capsids have found many applications in nanotechnology, biotechnology and medicine. (lu.se)

Profile antibody responses against 15 AAV capsid proteins in one single assay. (pepperprint.com)
Profile antibody responses against the most important wild type AAV capsid proteins with the PEPperCHIP ® Pan-AAV Capsid Protein Microarray. (pepperprint.com)
Antibody fingerprinting workflow using the PEPperCHIP® Pan-AAV Capsid Protein Microarray. (pepperprint.com)
Monitor antibody responses to AAV capsid proteins. (pepperprint.com)
Each WB/ELISA positive control contains purified viral protein that can serve as a binding target or an internal control for antibody validation or any antibody based assay such as western blotting or ELISA. (nanoexpo.eu)
Anti-Norovirus (VLP major capsid protein of GI and GII) monoclonal antibody, clone C2049N - 1mg - 1 vial is backordered and will ship as soon as it is back in stock. (karlan.com)

E1 protein has helicase activity for replication, and E2 encodes DNA-binding protein for regulation of transcription. (medscape.com)
In alphaviruses, this process is thought to be mediated, at least in part, by the viral protease (nsP2) which is responsible for processing the nonstructural polyproteins (P123 and P1234) into individual proteins (nsP1-nsP4), forming the viral replication complex. (bvsalud.org)
They facilitate many cellular functions, from DNA replication to molecular motion, energy production, and even the production of other proteins. (lu.se)

It consists of a CAPSID plus enclosed nucleic acid. (bvsalud.org)

X-ray structure of the Norwalk virus capsid, with the inset showing details of the structure of the subunits. (bcm.edu)
Virus capsids, filaments, flagel a, and other large protein complexes that are central to many biological processes are generated by protein self-assembly, whereby protein subunits spontaneously organize to form functional complexes. (lu.se)

In this application note, we profile capsid protein peptides from AAV5 and AAV9, digested with trypsin using a Biozen™ Peptide XB-C18 reversed phase column. (phenomenex.com)
15 different wild type AAV capsid proteins are converted into overlapping peptides and printed onto glass slides. (pepperprint.com)

The viral core protein retains protease activity, needed for cleavage from a protein precursor. (virology.ws)
Central to both Gag protein arrangements is the capsid (CA) protein, a domain of PrGag, which is cleaved from the precursor to yield a mature Gag protein composed of an N-terminal domain (NTD), a flexible linker region, and a C-terminal domain (CTD). (elsevierpure.com)

WB/ELISA positive control contain full lenght of major L1 capsid protein of Human papillomavirus 16 (HPV 16)(Cat. (nanoexpo.eu)
The three-dimensional structure of the membrane-bound form of the major coat protein of Pf1 bacteriophage was determined in phospholipid bilayers using orientation restraints derived from both solid-state and solution NMR experiments. (rcsb.org)

The detection of norovirus capsid protein was explored using three common MS-based methods: scanning of intact proteins, peptide mass fingerprinting, and peptide sequencing. (cdc.gov)

Interestingly, two types of hexameric protein arrangements were observed for each virus type. (elsevierpure.com)

Les échantillons de sérum de 140 patients susceptibles d'avoir contracté le virus de la dengue ont été analysés entre mai et octobre 2015 à l'aide du test ELISA et de l'amplification en chaîne par polymérase multiplexe. (who.int)

Mutagenesis studies confirm that hydrophobic residues in the centre of the three-helix bundle are crucial for capsid assembly and stability, and for viral infectivity. (nature.com)
This revealed that capsids differ widely in their stability. (elifesciences.org)
These observations reconcile seemingly contradictory observations made by different research groups about how this drug affects the stability of the capsid. (elifesciences.org)

CroV MCP gene was codon optimized, synthesized, and amplified by PCR followed by cloning into a bacterial expression system in order to obtain purified protein of high homogeneity. (utep.edu)
Lali K. Medina-Kauwe (2013) Development of adenovirus capsid proteins in targeting gene and drug delivery, Therapeutic Delivery , 4(2):267-77. (cedars-sinai.edu)
Rentsendorj A., J. Xie, M. MacVeigh, H. Agadjanian, S. Bass, S. Hamm-Alvarez and L.K. Medina-Kauwe (2006) Typical and Atypical Trafficking Pathways of Ad5 Penton Base Recombinant Protein: Implications for Gene Transfer, Nature - Gene Therapy 13(10):821-36. (cedars-sinai.edu)

CsCl density gradient centrifugation indicated that almost all of the NP protein in the cells had assembled into nucleocapsid-like structures. (nih.gov)
In contrast to previous structures determined solely in detergent micelles, the structure in bilayers contains information about the spatial arrangement of the protein within the membrane, and thus provides insights to the bacteriophage assembly process from membrane-inserted to bacteriophage-associated protein. (rcsb.org)
The cryo-electron-microscopy structures enable modelling by large-scale molecular dynamics simulation, resulting in all-atom models for the hexamer-of-hexamer and pentamer-of-hexamer elements as well as for the entire capsid. (nature.com)
The method can quickly elucidate the structure of many relevant proteins for humans, and for understanding structures relevant to disease, such as the structures of viral capsids. (lu.se)
In the final paper, we developed tools to design capsid-like proteins called cages - structures that can be used for drug delivery and vaccine design. (lu.se)

Role of Protein Charge Density on Hepatitis B Virus Capsid Formation" by Xinyu Sun, Dong Li et al. (uky.edu)
The role of electrostatic interactions in the viral capsid assembly process was studied by comparing the assembly process of a truncated hepatitis B virus capsid protein Cp149 with its mutant protein D2N/D4N, which has the same conformational structure but four fewer charges per dimer. (uky.edu)
and Liu, Tianbo, "Role of Protein Charge Density on Hepatitis B Virus Capsid Formation" (2018). (uky.edu)
The capsid not only protects the genetic material of the virus, but also helps in host-virus recognition, which is the critical initial step for viral infection. (utep.edu)
To investigate the requirements for budding of the paramyxovirus simian virus 5 (SV5), its M protein was expressed in mammalian cells, and it was found that SV5 M protein alone could not induce vesicle budding and was not secreted from cells. (nih.gov)
A protein shell called a capsid protects the virus during this journey. (elifesciences.org)
enables researchers to measure how molecules produced by host cells interact with the viral capsid, a structure that is fundamental for the virus to establish an infection. (elifesciences.org)
Norwalk virus is a small virus that consists of a single strand of RNA , which comprises the genetic material of the virus, surrounded by multiple copies of a single protein assembled into a protective coat that is called the capsid. (bcm.edu)
Al previous work in this area has relied on the properties of existing natural virus capsids. (lu.se)
In this project we wil use molecular engineering to custom- make virus capsids and going beyond the confines of Natures natural variety. (lu.se)
You wil study the self-assembly properties of the capsid protein and demonstrate that a fluorescent protein, GFP, can be selectively be encapsulated into synthetic virus capsids. (lu.se)
Nous avons déterminé la fréquence des différents sérotypes du virus de la dengue pour mettre en évidence son hyperendémicité à Rawalpindi (Pakistan). (who.int)

After large-scale expression in bacteria, ultra-high purity TF-MCP fusion protein was obtained by affinity chromatography and size exclusion chromatography. (utep.edu)

Once the provirus is integrated into the host cell DNA, it is transcribed using typical cellular mechanisms to produce viral proteins and genetic material. (msdmanuals.com)

There is also the phenomenon of HAP2 - a fusion protein that is used by many different eucaryotic organisms "all over" the phylogenetic tree (from Chlamydomonas to Bees) for mediating cell-cell fusion, predominantley of gametes. (virology.ws)
Turns out, that this protein look exactly like a class II viral fusion protein. (virology.ws)
The latter were a result of the emergent properties of the fusion protein. (psu.edu)
Mass spectrum (MS) and dynamic light scattering (DLS) were performed to analyze the molecular weight and hydrodynamic diameter of the fusion protein. (utep.edu)
The MS and DLS results suggested that pure TF-MCP fusion protein is in trimeric form, which indicates the proper folding of the MCP. (utep.edu)

Here we report the cryo-electron microscopy structure of a tubular HIV-1 capsid-protein assembly at 8 Å resolution and the three-dimensional structure of a native HIV-1 core by cryo-electron tomography. (nature.com)
Structure of the amino-terminal core domain of the HIV-1 capsid protein. (nature.com)
Crystal structure of dimeric HIV-1 capsid protein. (nature.com)
But the structure of the capsid presents a mystery. (elifesciences.org)
This method is based upon AlphaFold, a new AI tool that has revolutionized protein structure prediction. (lu.se)

Many of the studies were centered around viral protein capsids. (lu.se)

p24 is a component of the HIV particle capsid. (wikipedia.org)

The capsid-binding inhibitor PF74 accelerates capsid opening but stabilizes the remaining lattice. (elifesciences.org)

Uncoating of the metastable HIV-1 capsid is a tightly regulated disassembly process required for release of the viral cDNA prior to nuclear import. (elifesciences.org)

Anatomy7

Organisms68

Diseases5

Chemicals and Drugs20

Analytical, Diagnostic and Therapeutic Techniques and Equipment13

Phenomena and Processes24

Technology, Industry, Agriculture2

Information Science4

Encoding the major capsid protein2

Minor capsid proteins3

Genome9

Characterization1

Molecule4

Nucleocapsid proteins1

Structural15

Sequences4

Virion2

Antibodies3

Viruses10

Genes3

Assemblies4

Icosahedral1

Retroviral1

Aggregation1

Jelly roll1

Authentic3

Particles4

Antibody6

Replication3

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Subunits2

Peptides2

Precursor2

Major2

Peptide1

Interestingly1

ELISA1

Stability3

Gene3

Structures5

Virus12

Purity1

Genetic material1

Fusion protein5

Structure5

Viral protein1

Particle1

Lattice1

CDNA1