• Infections by protozoan parasites, such as Plasmodium falciparum or Leishmania donovani, have a significant health, social and economic impact and threaten billions of people living in tropical and sub-tropical regions of developing countries worldwide. (imperial.ac.uk)
  • In addition to identifying conserved sporozoite surface proteins identified by similar analyses of other Plasmodium species, our analysis identified several as-yet uncharacterized proteins, including a putative 6-Cys protein with no known ortholog in P. falciparum. (providence.org)
  • Identification and Characterization of Functional Human Monoclonal Antibodies to Plasmodium vivax Duffy-Binding Protein. (tropmedres.ac)
  • Genetic variability and natural selection at the ligand domain of the Duffy binding protein in Brazilian Plasmodium vivax populations. (ox.ac.uk)
  • Fernández-Robledo JA, Vasta GR (2010) Production of recombinant proteins from protozoan parasites. (springer.com)
  • In this review we summarize the latest insights into protein lipidation in protozoan parasites. (imperial.ac.uk)
  • The MB2 protein is distributed as a approximately 120-kDa moiety on the surface of sporozoites and is imported into the nucleus of blood-stage parasites as a approximately 66-kDa species. (ox.ac.uk)
  • Sample preparation (protein and drug-resistant L. tropica parasites extraction) niasis, early diagnosis and appropriate [8]. (who.int)
  • Reprinted from Cell 81, Collins, Kathleen, et al, "Purification of Tetrahymena Telomerase and Cloning of Genes Encoding the Two Protein Components of the Enzyme", pg. (jhu.edu)
  • Regulation of gene expression is primarily controlled by changes in the proteins that occupy genes' regulatory elements. (nature.com)
  • The yeast two-hybrid system is a technique used to identify genes that code for proteins that are associated with a certain protein in vivo. (cmich.edu)
  • Further, we constructed protein interaction networks of the genes identified by the feature selection method and gene ontology analysis elucidated the role of the proteins in the progression of the parasite through its life cycle. (fortunepublish.com)
  • A fragment of the Trypanosoma brucei ZC3H41 protein encompassing the ATP-dependent RNA helicase domain was successfully subcloned for expression in a bacterial system (Escherichia coli). (iucc.ac.il)
  • Izhaki-Tavor, LS & Dessau, M 2020, ' ATP-dependent RNA helicase domain of the ZC3H41 protein from Trypanosoma brucei: Expression, purification and crystallization ', Acta Crystallographica Section F:Structural Biology Communications , vol. 76, pp. 604-608. (iucc.ac.il)
  • Genome-wide subcellular protein map for the flagellate parasite Trypanosoma brucei. (ox.ac.uk)
  • Trypanosomatid parasitic protozoa such as Leishmania and Trypanosoma brucei make extensive use of this method of protein surface attachment. (gla.ac.uk)
  • The most advanced malaria vaccine for P. falciparum (RTS,S, a subunit vaccine containing of a portion of the major sporozoite surface protein) conferred limited protection in Phase III trials, falling short of WHO-established vaccine efficacy goals. (providence.org)
  • It is also thought that a multivalent vaccine comprising multiple sporozoite surface antigens will provide better protection, but a comprehensive analysis of proteins in P. vivax sporozoites is not available. (providence.org)
  • With the second week serum, 34 kDa protein (p34) was detected uniquely, and all antigens of T. gondii were detected with the sera from 3 or 4 weeks. (koreamed.org)
  • These antibodies detected recombinant protein but failed to detect protein in L. mexicana cell lysates. (gla.ac.uk)
  • A protein of 38-40 kDa, however, was detected in cell lysates of T. brucei with the antirecombinant GPI8 antibodies. (gla.ac.uk)
  • Demain AL, Vaishnav P (2009) Production of recombinant proteins by microbes and higher organisms. (springer.com)
  • Understanding their complex cell architecture and life cycle is challenging because, as with most eukaryotic microbes, ~50% of genome-encoded proteins have completely unknown functions. (ox.ac.uk)
  • In this context, it is worth noting that the production of recombinant proteins by photosynthetic organisms offer both eukaryotic (nuclear) and prokaryotic (chloroplast) alternatives, along with the advantage of an autotrophic nutrition. (springer.com)
  • Also, unicellular green algae, in particular Chlamydomonas reinhardtii , have been proposed as candidate organisms for the production of recombinant proteins. (springer.com)
  • Hacker DL, De Jesus M, Wurm FM (2009) 25 years of recombinant proteins from reactor-grown cells - where do we go from here? (springer.com)
  • The first phase involves elucidating the three-dimensional structure of parasitic protozoan proteins that are potential drug targets, allowing the identification of inhibiting compounds that block the action of pathogenic proteins. (astellas.com)
  • Here, using fluorescence microscopy and cell lines expressing endogenously tagged proteins, we mapped the subcellular localization of 89% of the T. brucei proteome, a resource we call TrypTag. (ox.ac.uk)
  • We developed genomic locus proteomics (GLoPro), in which we combine CRISPR-based genome targeting, proximity labeling, and quantitative proteomics to discover proteins associated with a specific genomic locus in native cellular contexts. (nature.com)
  • To inform sporozoite-based vaccine development, we employed mass spectrometry-based proteomics to identify nearly 2,000 proteins present in P. vivax salivary gland sporozoites. (providence.org)
  • Untargeted mass spectrometry (MS)-based proteomics reveals that some proteins also expressed in saliva were detected with greater expression differences in blood plasma when comparing mpox patients and healthy subjects, suggesting a promising alternative to be applied in screening or diagnostic platforms for mpox salivary diagnostics coupled to OMICs. (bvsalud.org)
  • ABSTRACT In order to define the protein expressional changes related to the process of meglumine antimoniate resistance in anthroponotic cutaneous leishmaniasis (CL), we performed a comparative proteomics analysis on sensitive and resistant strains of Leishmania tropica isolated from Iranian CL patients. (who.int)
  • The study shows the usefulness of proteomics in identifying proteins that may express differences between sensitive and resistant L. tropica isolates. (who.int)
  • We describe the purification of telomerase and the cloning of cDNAs encoding two protein subunits from the ciliate Tetrahymena. (jhu.edu)
  • The MPXV uses the host cell's rough endoplasmic reticulum, ribosomes, and cytoplasmic proteins to replicate its genome and synthesize virions for cellular exit. (bvsalud.org)
  • Cell proteins were analysed with 2-dimensional electrophoresis and differentially expressed proteins were identified by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry. (who.int)
  • We discuss how recent chemical proteomic approaches have delivered the first global overviews of protein lipidation in these organisms, contributing to our understanding of the role of this PTM in critical metabolic and cellular functions. (imperial.ac.uk)
  • Additionally, the sporozoite surface proteins CSP and TRAP, which were recently discovered to be glycosylated in P. falciparum salivary gland sporozoites, were also observed to be similarly modified in P. vivax sporozoites. (providence.org)
  • Many eukaryotic cell surface proteins are attached to the plasma membrane by glycosylphosphatidylinositol (GPI) anchors. (gla.ac.uk)
  • We describe extensive use of histidine kinase-based two-component systems and tyrosine kinase signaling, the presence of bacterial and plant type photoreceptors (phytochromes, cryptochrome, and phototropin) and of plant-type pentatricopeptide repeat proteins, as well as metabolic pathways, and a cell cycle control system typically found in more complex eukaryotes. (duke.edu)
  • Quantitative comparison of the P. vivax and P. falciparum salivary gland sporozoite proteomes revealed a high degree of similarity in protein expression levels, including among invasion-related proteins. (providence.org)
  • Finally, we employed chemical labeling of live sporozoites to isolate and identify 36 proteins that are putatively surface-exposed on P. vivax salivary gland sporozoites. (providence.org)
  • Engagement of the highly polymorphic domain II of the P. vivax Duffy-binding protein (DBPII) with the erythrocyte's Duffy Ag receptor for chemokines (DARC) is essential. (tropmedres.ac)
  • Invasion of host blood cells by P. vivax mainly depends on a type I membrane protein called Duffy binding protein (PvDBP). (ox.ac.uk)
  • The crystal structure of the parasite protein has been solved and shows prospects for design of inhibitors to be used as leads for development of new drugs. (port.ac.uk)
  • The perceived importance of GPI-anchored proteins makes the GPI biosynthetic pathway a good target for anti-parasite chemotherapy. (gla.ac.uk)
  • This Review summarizes the more recent biochemical, cellular and mouse model studies that underscore the importance of the ER acetylation process in maintaining protein homeostasis and autophagy within the secretory pathway, and its impact on developmental and age-associated diseases. (biologists.com)
  • Proteolytic processing is favored as the mechanism regulating the distinct subcellular localization of the MB2 protein. (ox.ac.uk)
  • This method involves designing drugs for the target protein by analyzing and comparing the structure of various compounds and complex conjugates in order to develop an overall understanding of the mechanism by which protein activity is inhibited (or activated). (astellas.com)
  • This perspective focuses on two areas that have yielded new useful information during the last 20 years: (i) structure-activity relationship (SAR) studies of contact allergy based on the concept of hapten-protein binding and (ii) mechanistic investigations regarding activation of nonsensitizing compounds to contact allergens by air oxidation or skin metabolism. (cdc.gov)
  • We have cloned and characterized the Dictyostelium discoideum repE gene, a homolog of the human xeroderma pigmentosum (XP) group E gene which encodes a UV-damaged DNA binding protein. (nih.gov)
  • We are studying copine protein function in the model organism protozoan Dictyostelium discoideum. (cmich.edu)
  • To fully understand the function of copines in Dictyostelium, it is critical to identify their target proteins. (cmich.edu)
  • The proteins DAip1, CorA, and EfaAl are associated with actin filament organization in Dictyostelium suggesting that CpnA may have a role in regulating the actin cytoskeleton. (cmich.edu)
  • Here, we show that mouse Stbd1 is a transmembrane endoplasmic reticulum (ER)-resident protein with the capacity to induce the formation of organized ER structures in HeLa cells. (imperial.ac.uk)
  • There is some overlap in fluorescence patterns between tagged QM and the endoplasmic reticulum marker protein BiP. (gla.ac.uk)
  • Proteins found in any species of protozoan. (bvsalud.org)
  • Nevertheless, orthologs with significantly different expression levels between the two species could be identified, as well as highly abundant, species-specific proteins with no known orthologs. (providence.org)
  • The erythrocyte-binding motif of PvDBP is a 170 amino-acid stretch located in its cysteine-rich region II (PvDBPII), which is the most variable segment of the protein. (ox.ac.uk)
  • Importantly, the amino acid diversity of EMAs is distributed throughout the length of the proteins. (biomedcentral.com)
  • Proteins: Structure, Function and Bioinformatics. (illinois.edu)
  • SCOPe: Structural Classification of Proteins - extended. (berkeley.edu)
  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. (springer.com)
  • The second phase consists of the structural analysis of target proteins in complex with the inhibitory compound. (astellas.com)
  • Structural data obtained through the successful application of this collaborative research will contribute to the discovery of new drugs for the treatment of parasitic protozoan diseases. (astellas.com)
  • The collected data revealed preliminary structural information regarding this newly discovered protein. (iucc.ac.il)
  • Thus, it is important to note that the identification and quantification of salivary DNA, RNA, lipid, protein, and metabolite can provide novel non-invasive biomarkers through the use of OMICs platforms aiding in the early detection and diagnosis of MPXV infection. (bvsalud.org)
  • The target of this research is infectious diseases caused by parasitic protozoans, namely leishmaniasis※3, Chagas disease※4 and Sleeping sickness※5, and will be divided into two major phases. (astellas.com)
  • Our preliminary study using 2D isolates were recovered from liq- new cases throughout the world cu- gel electrophoresis in drug sensitive/ uid nitrogen, and sub-cultured in taneous leishmaniasis (CL) remains resistant strains of L. tropica showed RPMI1640 medium (Gibco/BRL) a serious public health problem in that some proteins were differentially supplemented with 10% fetal bovine numerous countries [1,2]. (who.int)
  • This information is based on mapping of SMART genomic protein database to KEGG orthologous groups. (embl.de)
  • Post-translational modifications (PTMs) are important modulators of biology and inhibition of protein lipidation has emerged as a promising therapeutic strategy for treatment of parasitic diseases. (imperial.ac.uk)
  • Prion diseases are rare progressive, fatal, and currently untreatable degenerative disorders of the brain (and rarely of other organs) that result when a protein changes into an abnormal form called prion. (msdmanuals.com)
  • At the primary sequence level, the two telomerase proteins share only limited homologies with other polymerases and polymerase accessory factors. (jhu.edu)
  • Sequence comparison of L. mexicana, yeast and human GPI8 proteins identified two potential active site cysteine residues. (gla.ac.uk)
  • Protein post-translational modifications (PTMs) take many shapes, have many effects and are necessary for cellular homeostasis. (biologists.com)
  • In order for a cell to respond to changes in internal and external environmental factors, a broad range of protein co- and post-translational modifications have evolved to expand upon the relatively static properties encoded in protein side-chains. (biologists.com)
  • Analysis of protein post-translational modifications revealed extensive phosphorylation of glideosome proteins as well as regulators of transcription and translation. (providence.org)
  • The retroviral oncogene v-myb, and its cellular counterpart c-myb, encode nuclear DNA-binding proteins. (embl.de)
  • The single-exon, single-copy MB2 gene is predicted to encode a protein with an M(r) of 187,000. (ox.ac.uk)
  • To evaluate whether these pathogens were from different lineages, cluster analysis based on seven microsatellite genotyping markers and the merozoite surface protein 1 (pkmsp1) gene was carried out. (ox.ac.uk)
  • There are 139666 SANT domains in 88628 proteins in SMART's nrdb database. (embl.de)
  • Copine proteins consists of two C2 domains at the N terminus followed by an "A domain" similar to the von Willebrand-Integrin A domain. (cmich.edu)
  • Starch binding domain-containing protein 1 (Stbd1) is a carbohydrate-binding protein that has been proposed to be a selective autophagy receptor for glycogen. (imperial.ac.uk)
  • The SARS-CoV-2 entry into host cells is mainly mediated by the interactions between the viral spike protein (S) and the ACE-2 cell receptor, which are highly glycosylated. (bvsalud.org)
  • Compared to nuclear transformation, chloroplast transformation generally allows a higher level of accumulation of the recombinant protein of interest. (springer.com)
  • Houdebine L-M (2009) Production of pharmaceutical proteins by transgenic animals. (springer.com)
  • A comparison of their known functions has identified, besides a common role within protein folding, multiple roles for the cyclophilins within pre-mRNA splicing and cellular signalling, and within transcription and cell cycle regulation for the parvulins. (biomedcentral.com)
  • For example, penicillin targets families of penicillin-binding proteins, and fluoroquinolones such as ciprofloxacin target DNA-binding proteins. (illinois.edu)
  • These data show that GPI-anchored proteins of L. mexicana are not essential for growth of promastigotes, invasion of macrophages by promastigotes, or infection of mice. (gla.ac.uk)
  • The predicted protein encodes a leucine zipper motif. (nih.gov)
  • This is the first iso-enzyme and the second protein analysis to indicate strain differences in B. hominis. (nih.gov)
  • The peptidyl-prolyl cis / trans isomerase (PPIase) class of proteins is traditionally comprised of three distinct protein families, the cyclophilins (cyclosporin A binding proteins), FKBPs (FK506 binding proteins) and parvulins, that are linked by their shared ability to catalyse the bond preceding a proline residue between its cis and trans forms. (biomedcentral.com)
  • The fusion protein, however, was unable to restore GPI8 function in the null mutant, therefore data on its localisation cannot be inferred. (gla.ac.uk)
  • Taxonomic distribution of proteins containing SANT domain. (embl.de)
  • The complete taxonomic breakdown of all proteins with SANT domain is also avaliable . (embl.de)
  • Click on the protein counts, or double click on taxonomic names to display all proteins containing SANT domain in the selected taxonomic class. (embl.de)
  • Percentage points are related to the number of proteins with SANT domain which could be assigned to a KEGG orthologous group, and not all proteins containing SANT domain. (embl.de)
  • The MB2 protein has an amino-terminal basic domain, a central acidic domain, and a carboxyl-terminal domain with similarity to the GTP-binding domain of the prokaryotic translation initiation factor 2. (ox.ac.uk)