• inhibitor
  • Furthermore, PKC phosphorylation of SHP2 was completely blocked by the PKC inhibitor bisindolylmaleimide and was not detectable when SHP2 was co- overexpressed with kinase negative mutants of PKCbeta1 and - beta2. (mpg.de)
  • The protein kinase A inhibitor Rp-cyclic adenosine monophosphate (100-150 microM) did not block the adenosine-dependent reduction of the mEPSC frequency, showing that adenosine is not depressing synaptic transmission via a protein kinase A (PKA)-dependent mechanism. (mysciencework.com)
  • Administration of the NAD(P)H oxidase inhibitor diphenyleneiodonium, apocynin, the protein kinase C (PKC) inhibitor chelerythrine or staurosporin or the removal of extracellular Ca 2+ during high P i treatment prevented the increases in O 2 ·− production, whereas administration of losartan or captopril had no effect. (ahajournals.org)
  • isoforms
  • To study whether protein kinase C (PKC) isoforms can interact with protein-tyrosine-phosphatases (PTPs) which are connected to the insulin signaling pathway, we co-overexpressed PKC isoforms together with insulin receptor, docking proteins, and the PTPs SHP1 and SHP2 in human embryonic kidney (HEK) 293 cells. (mpg.de)
  • In order to investigate any effect of truncated mutant huntingtin (tNhtt) aggregation on protein kinase C (PKC) signaling in Huntington's disease (HD), we studied a possible association of PKC isoforms with the aggregates using cellular and transgenic models of HD. (mysciencework.com)
  • vitro
  • Transcription and translation of this cDNA in vitro generates a 100 kDa protein similar to the human gene product ERK3. (biochemj.org)
  • 15. A method of inhibiting the expression of human protein kinase C-α in vitro comprising contacting human cells with a therapeutically effective amount of an antisense oligonucleotide 5 to 50 nucleotides in length, said antisense oligonucleotide comprising a nucleotide sequence complementary to a portion of the sequence set forth in SEQ ID NO: 105. (google.com.au)
  • role
  • Also, we aimed to elucidate the contribution of possible vascular sources of O 2 ·− , including NAD(P)H oxidases, 4,5 and the role of mechanosensitive pathways, such as Ca 2+ and protein kinase C (PKC)-dependent signaling mechanisms. (ahajournals.org)