KinaseKinasesBacterialHumanized histidine-richPlasmodiumSignal transductionResiduesIntrinsically disorderedRecombinantStructural ClassifiPurificationSubstancesAcidsDecarboxylasePeptidesIsolateMacromoleculesEnzymeMoleculePeptideKnob-associatedFoldsCytoplasmicGeneMetabolismAmino acidCarbohydratesAssaysFoldLigandsPrecursorGenesMolecularConcomitantWhey proteinMutationMorphogenesisResiduePhenotypeSolubilityPolyhistidineTypicallyReceptorsSequencesMRNAServingEscherichiaAntibodyBindZincAffinityEukaryoticConcentrationsGrams of proteinLactoseSolubleHigh-proteinCopperYieldPowder
Kinase10
- Look up protein kinase in Wiktionary, the free dictionary. (wikipedia.org)
- Protein histidine kinase may refer to: Histidine kinase, an enzyme Protein-histidine tele-kinase, an enzyme Protein-histidine pros-kinase, an enzyme This disambiguation page lists articles associated with the title Protein histidine kinase. (wikipedia.org)
- The objectives of the proposed Transnational Network are to bring together scientists from academia and industry working in relevant multidisciplinary fields to develop integrated strategies to overcome the permeability barrier of Gram-negative cell envelopes and further develop newly discovered histidine kinase inhibitors as anti-virulence therapies. (wur.nl)
- Heme-based sensors are a recently discovered functional class of heme proteins that serve to detect physiological fluctuations in oxygen (O 2 ), carbon monoxide (CO), or nitric oxide (NO). Many of these modular sensors detect heme ligands by coupling a histidine-protein kinase to a heme-binding domain. (elsevierpure.com)
- We identified an atypical phase-variable signal transduction system consisting of a histidine kinase and two response regulators, named herein colony morphology regulators RST (CmrRST), which mediates the switch in colony morphology and motility behaviors. (nih.gov)
- In most bacteria, a histidine kinase (Hk2) detects a signal, and passes that signal to a response regulator protein (Rrp2) which then activates gene expression by sigma54. (usda.gov)
- We found that a B. burgdoferi strain lacking the histidine kinase, Hk2, still expresses sigma54 dependent genes. (usda.gov)
- Further experiments showed that rpoN is required for stationary phase expression of rpoS in B. burgdorferi, but that the histidine kinase Hk2 is not absolutely essential. (usda.gov)
- This suggested that another histidine kinase or small molecular weight phosphate donors could activate Rrp2, providing multiple signaling pathways for modulating expression of the sigma54/sigmaS regulon in B. burgdorferi. (usda.gov)
- In the poster section, Joanna Shisler (University of Illinois at Urbana-Champaign [UIUC], Urbana) reported that the modified virus, Ankara, activates nuclear factor κB through the mitogen-activated protein kinase, extracellular signal–regulated kinase (MEK)/extracellular signal–regulated kinase (ERK) pathway, possibly facilitating the host immune response. (cdc.gov)
Kinases5
- HPts function in histidine-aspartate phosphorelays in which they mediate the signal from sensory kinases (usually membrane proteins) to RRs in the nucleus. (rcsb.org)
- We have previously identified a panel of inhibitors targeting bacterial histidine kinases in bacteria that inhibit targets involved in the regulation of virulence and stress response pathways in bacterial pathogens (Velikova et al. (wur.nl)
- The activities of this Transnational Network are aimed at aligning international research activities and combining expertise to devise the most efficient strategy to further develop new anti-infective drugs targeted to histidine kinases. (wur.nl)
- Histidine-protein kinases function in partnership with cognate response-regulator substrate(s): usually transcription factors that they activate by phosphorylation. (elsevierpure.com)
- We used loss-of-function mutants to study three Arabidopsis thaliana sensor histidine kinases, AHK2, AHK3, and CRE1/AHK4, known to be cytokinin receptors. (nih.gov)
Bacterial3
- In addition to its use for small-scale protein purification, the columns are also ideal for screening large numbers of small-scale bacterial lysates and for optimising protein purification conditions. (outsourcing-pharma.com)
- Despite its importance in central metabolism and bacterial cell signaling, protein histidine phosphorylation has remained elusive with respect to its extent and functional roles in biological systems because of the lack of adequate research tools. (princeton.edu)
- This makes them suitable for the purification of proteins from eukaryotic samples, thereby extending the use of polyhistidine proteins from bacterial to more complex expression systems. (genengnews.com)
Humanized histidine-rich1
- In this study, we have explored several humanized histidine-rich peptides in tumor-targeted modular proteins, which can specifically bind and be internalized by the target cells through the tumoral marker CXCR4. (upc.edu)
Plasmodium3
- Location of study sites at Ghindae and Massawa Hospitals, Eritrea, for analysis of a major threat to malaria control programs by Plasmodium falciparum lacking histidine-rich protein 2. (cdc.gov)
- Comparative analysis of the Plasmodium falciparum histidine-rich proteins HRP-I, HRP-II and HRP-III in malaria parasites of diverse origin. (ox.ac.uk)
- Plasmodium falciparum-infected erythrocytes (IRBC) synthesize 3 histidine-rich proteins: HRP-I or the knob-associated HRP, HRP-II and HRP-III or SHARP. (ox.ac.uk)
Signal transduction1
- Histidine-containing phosphotransfer proteins (HPts) take part in hormone signal transduction in higher plants. (rcsb.org)
Residues7
- Histidine residues have been suggested as triggers due to their approximate textbook pK(a) value of 6.1 for their side chains. (uni-koeln.de)
- We present here an extensive theoretical and experimental study of the 14 histidine residues present in canine HSP47, where we have mutated all histidine residues in the collagen binding interface and additionally all of those that were predicted to undergo a significant change in protonation state between pH 7 and 6. (uni-koeln.de)
- In polyhistidine tagging, 6 to 10 histidine residues are added recombinantly to either the protein's C- or N-terminus. (genengnews.com)
- However, according to Dr. Boronowsky, when membrane proteins are being investigated, 10 or even 14 residues might be employed to overcome the masking effects of any detergent that might have been added to improve protein solubilization. (genengnews.com)
- Analyses of the albumin treated with acrolein for amino acids revealed a disappearance of lysine and histidine residues with concomitant appearance of four new peaks. (cdc.gov)
- The results indicated that although lysine and histidine residues were modified, such modification did not alter the functions or biological properties of albumin, such as binding to palmitic-acid or bromcresol-green. (cdc.gov)
- Aromatic residues contribute a significant part (roughly 25% of the volume on average) of the hydrophobic core of proteins, and are prevalent in protein binding interfaces. (lu.se)
Intrinsically disordered1
- Histatin 5 is a histidine-rich, intrinsically disordered, multifunctional saliva protein known to act as a first line of defense against oral candidiasis caused by Candida albicans. (lu.se)
Recombinant7
- Therefore, obtaining a high amount of the desired his- tag recombinant proteins always requires a balance between expression rate and accumulation. (magiran.com)
- Optimization of the protocols in Ni-NTA affinity chromatography of recombinant proteins with denaturants for MiRGD, GNH and HNH brought about with increase of purified protein concentrations from 0.21, 0.4, and 0.16 mg/ml in the initial attempt to 2.9, 3.4 and 2.6 mg/ml in the optimized experiment respectively. (magiran.com)
- Synthesis and secretion of recombinant tick-borne encephalitis virus protein E in soluble and particulate form. (microbiologyresearch.org)
- Recombinant expression of proteins of interest in Escherichia coli is an important tool in the determination of protein structure. (iucr.org)
- There is extensive literature on the variables leading to insoluble recombinant expression of proteins. (iucr.org)
- Quality control test: Antibody Reactive Against Recombinant Protein. (novusbio.com)
- NME2 (NP_002503, 51 a.a. ~ 152 a.a) partial recombinant protein with GST tag. (novusbio.com)
Structural Classifi1
- SCOPe: Structural Classification of Proteins - extended. (berkeley.edu)
Purification9
- To prevent the binding of host cell proteins with exposed histidines, it is essential to include imidazole at a low concentration in the sample and binding buffer ( Optimizing purification of histidine-tagged proteins ). (sigmaaldrich.com)
- GE Healthcare has announced the availability of its single-use spin column designed for purification of histidine-tagged proteins using a standard microcentrifuge. (outsourcing-pharma.com)
- It saves researchers time as it achieves direct protein purification in 10 minutes per run. (outsourcing-pharma.com)
- By reducing the total purification time, the spin column procedure minimises degradation and oxidation of sensitive protein samples and preserves the activity of the target protein. (outsourcing-pharma.com)
- Poly-histidine peptides such as H6 (HHHHHH) are used in protein biotechnologies as purification tags, pro- tein-assembling agents and endosomal-escape entities. (upc.edu)
- We were particularly interested in exploring how protein purification, self-assembling and endosomal escape perform in proteins containing the variant histidine-rich tags. (upc.edu)
- The standard purification process can also be optimized based on the Physicochemical properties, such that, in addition to increasing yield in the expression step, the activity and concentration of the desired protein is maintained in the purification step. (magiran.com)
- In this study, we obtained the results of extraction of three different peptides MiRGD, GNH and HNH, and two proteins firefly mutant red-emitter luciferase, and human DT-diaphorase proteins by altering these variables on expression as well as purification conditions. (magiran.com)
- Cube Biotech offers PureCube resins for the purification of His-tagged proteins. (genengnews.com)
Substances2
- Each lot of Essential Protein is rigorously screened for over 250 banned substances in sport-including stimulants, narcotics, and diuretics. (ritual.com)
- The body uses these basic units to build substances it needs for growth, maintenance, and activity (including other carbohydrates, proteins, and fats). (msdmanuals.com)
Acids14
- Amino acids are important biological units that are sometimes called the 'building blocks' of proteins because many amino acids together form proteins. (ivyroses.com)
- Histidine is one of those amino acids that are necessary and cannot be produced within the body. (ivyroses.com)
- Pea protein is easy for the body to use and convert into essential amino acids. (vitanetonline.com)
- For athletes doing a purely strength workout (e.g. a weight training session in the gym), consuming 1 scoop of Whey Protein immediately after the workout is a great to provide the lean muscle tissue with the amino acids it needs to repair and rebuild. (hammernutrition.com)
- Consuming 1 scoop of Whey Protein prior to bedtime is also a highly beneficial practice-the body receives an excellent dose of amino acids, which it will use during a time (sleep) when it does many of its reparation/recuperative processes, and the glutamine will elevate serum human growth hormone (hGH) levels, which has anabolic (muscle building) effects. (hammernutrition.com)
- The protein also supplies the amino acids needed to help repair and rebuild the muscle, and the glutamine supports healthy immunity. (hammernutrition.com)
- Our select line of products has everything you need to help support your fitness journey, from creatine to amino acids to protein powders. (puritan.com)
- Whey protein contains a high concentration of Branched Chain Amino Acids (BCAA's), which are important for efficient muscle metabolism. (well.ca)
- The aim of the present study was to characterize the changes occurring in amino acids profile of camel milk protein during the 1st month of lactation and seek to justify such dynamics of change in relation to the specific needs of growing neonates. (scialert.net)
- 2005). Proteins of camel milk contain all essential amino acids and in its fats there are unsaturated aliphatic acids. (scialert.net)
- The objective of this study was to characterize the changes occurring in amino acids of Camel milk protein and determine the dynamic relation ships among IGF-I, T 3 and T 4 during the 1st month of lactation and seek to justify such dynamics of change in relation to the specific needs of the growing neonates. (scialert.net)
- In summary, the person is trying to get their head around the basic structure, formulas and meanings of carbohydrates, proteins, amino acids and lipids. (physicsforums.com)
- I have been trying to get my head around the basics for these four (Carbohydrates, Proteins, Amino Acids and Lipids) for about a week and I just don't understand the basic structure, formulas and such. (physicsforums.com)
- You say amino acids and proteins. (physicsforums.com)
Decarboxylase1
- Precursor of histamine by action of histidine decarboxylase. (sigmaaldrich.com)
Peptides3
- The pleiotropic properties of such peptides make them appealing to design protein-based smart materials or nanoparticles for imaging or drug delivery to be produced in form of re- combinant proteins. (upc.edu)
- In order to distinguish these proteins immunochemically we prepared monoclonal antibodies which react with HRP-I, HRP-II and HRP-III, and rabbit antisera against synthetic peptides derived from the HRP-II and HRP-III sequences. (ox.ac.uk)
- Role of histidine for charge regulation of unstructured peptides at interfaces and in bulk. (lu.se)
Isolate10
- ALLWHEY GOLD delivers and ultra-high quality whey isolate-concentrate protein blend to help rebuild your muscles after intense training. (vitacost.com)
- Yellow Pea Protein Isolate. (vitanetonline.com)
- Doing so is easy with Hammer Nutrition's Whey Protein, which contains 100% whey protein isolate, the most bioavailable protein on the planet. (hammernutrition.com)
- Every serving (1 scoop) of Whey Protein contains 15 grams of 100% whey protein isolate and 6 grams of glutamine. (hammernutrition.com)
- Every serving (2 scoops) of Recoverite contains 10 grams of the same exact whey protein isolate that's in Whey Protein, as well as 3 grams of glutamine. (hammernutrition.com)
- Each scoop of Whey Protein contains 15 grams of whey protein isolate, which, compared to other protein sources, is the superior protein source for a number of reasons (see the articles Hammer Whey Protein and Stay Strong and Stay Healthy for more). (hammernutrition.com)
- Other protein powders may have more grams of protein in a serving, but oftentimes they're mostly a blend of a bit of whey protein isolate, whey protein concentrate (a lower-quality form of whey protein), and casein protein, the latter being one that we do not consider to be a quality protein source. (hammernutrition.com)
- a 100% vegetable protein isolate that’s big on benefits and offers an alternative to dairy, soy and whey-based products. (mrsupplement.com.au)
- NOW Sports Whey Protein Isolate Powder is a high quality protein supplement that is both bioavailable and easily digested. (well.ca)
- NOW Whey Protein Isolate is ideal for active individuals. (well.ca)
Macromolecules2
- It also has a role in binding of heme groups, macromolecules and phosphate groups in the binding sites of proteins. (sigmaaldrich.com)
- To cause sensitization, a chemical must bind to macromolecules (proteins) in the skin. (cdc.gov)
Enzyme4
- Histidinemia is caused by the shortage (deficiency) of the enzyme that breaks down histidine. (medlineplus.gov)
- HAL gene mutations lead to the production of a histidase enzyme that cannot break down histidine, which results in elevated levels of histidine in the blood and urine. (medlineplus.gov)
- And any enzyme or other protein you come across, you can look it up on Internet. (physicsforums.com)
- Histidine and tyrosine also play prominent roles in enzyme catalysis. (lu.se)
Molecule1
- Histidase breaks down histidine to a molecule called urocanic acid. (medlineplus.gov)
Peptide9
- Among the tested candidates, the peptide H5E (HEHEHEHEH) is promising as a good promoter of endosomal escape of the associated full- length protein upon endosomal internalization. (upc.edu)
- Endocrine factors such as insulin like growth factor and thyroid hormones (Triiodothyronone (T 3 ) and Thyroxine (T 4 ) can also influence the time point of gut closure for protein and peptide absorption (Hadron et al . (scialert.net)
- Then get on to how they typically interact with each other and how the peptide bond folds and how proteins fold and how this makes them work and some examples of them working. (physicsforums.com)
- Here we are investigating the role of the histidines in more detail by defining a library of variants of the peptide, where. (lu.se)
- Here we are investigating the role of the histidines in more detail by defining a library of variants of the peptide, where the former have been replaced by the pH-insensitive amino acid glutamine. (lu.se)
- By using experimental techniques such as circular dichroism, small angle X-ray scattering, quartz crystal microbalance with dissipation monitoring, and neutron reflectometry, it was determined that changing the number of histidines in the peptide sequence did not affect the structure of the peptide dissolved in solution. (lu.se)
- However, it was shown to affect the penetration depth of the peptide into the bilayer, where all variants except the one with zero histidines were found below the bilayer. (lu.se)
- A decrease in the number of histidine from the original seven to zero decreases the ability of the peptide to penetrate the bilayer, and the peptide is then also found residing within the bilayer. (lu.se)
- We hypothesize that this is due to the ability of the histidines to charge titrate, which charges up the peptide, and enables it to penetrate and translocate through the lipid bilayer. (lu.se)
Knob-associated1
- mAb 18.2, which recognises the Knob Associated Histidine Rich protein (KAHRP) is now available. (malariaresearch.eu)
Folds1
- In eukaryotes, genomic DNA, which is a very long double helix containing all the genetic information, wraps around a globular protein called a histone and folds it many times before being contained within the nucleus. (phys.org)
Cytoplasmic2
- The numer- ical modelling of cell penetration and endosomal escape of the tested proteins has revealed a negative relationship between the amount of protein internalized into target cells and the efficiency of cytoplasmic release. (upc.edu)
- All copper-transporting ATPases have a histidine residue in the large cytoplasmic loop adjacent to the ATP-binding domain. (medscape.com)
Gene4
- Short-lived proteins control gene expression in cells to carry out a number of vital tasks, from helping the brain form connections to helping the body mount an immune defense. (phys.org)
- Co-transcription of this gene and the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) which encode a fusion protein comprised of sequence sharing identity with each individual gene product. (novusbio.com)
- The coordinated regulation of gene expression is required for the transmission and survival of Borrelia burgdorferi in different hosts, and the sigma factor RpoS (sigmaS), as regulated by RpoN (sigma54), has been shown to regulate key proteins in these processes. (usda.gov)
- The etiopathogenesis of the zinc deficiency occurs as a result of loss-of-function mutations of the zinc-ligand binding protein ZIP4 encoded by the SLC39A4 ( solute carrier 39A) gene located on band 8q24.3. (medscape.com)
Metabolism1
- This perspective focuses on two areas that have yielded new useful information during the last 20 years: (i) structure-activity relationship (SAR) studies of contact allergy based on the concept of hapten-protein binding and (ii) mechanistic investigations regarding activation of nonsensitizing compounds to contact allergens by air oxidation or skin metabolism. (cdc.gov)
Amino acid3
- Histidine is an essential amino acid . (ivyroses.com)
- L-Histidine is an essential amino acid. (sigmaaldrich.com)
- Whey protein is considered to have the highest Biological Value of any protein source - superior in essential amino acid content to beef, milk, casein or soy. (well.ca)
Carbohydrates3
- Whey Protein contains only 0-1 grams of naturally occurring carbohydrates (no added carbohydrates). (hammernutrition.com)
- Carbohydrates, proteins, and fats are the main types of macronutrients in food (nutrients that are required daily in large quantities). (msdmanuals.com)
- Thus, they tend to provide energy to the body more slowly than simple carbohydrates but still more quickly than protein or fat. (msdmanuals.com)
Assays1
- This antibody was successfully used in ELISA, western blotting, dot blot assays and immunoprecipitation and in detection and identification of histidine-phosphorylated proteins from native cell lysates when coupled with MS analysis. (princeton.edu)
Fold2
- The protein fold consists of six α helices, four of which form a C-terminal helix bundle. (rcsb.org)
- They may feature a PAS fold, as in the Bradyrhizobium japonicum and Sinorhizobium melitoti FixL proteins, or a GAF fold, as in the Mycobacterium tuberculosis DevS and DosT proteins. (elsevierpure.com)
Ligands1
- Chemotaxis is triggered by the binding of ligands (chemoeffectors) to chemoreceptors called methyl-accepting chemotaxis proteins (MCPs) and transducer-like proteins (Tlps). (nature.com)
Precursor1
- The mature flavivirus particle comprises a nucleocapsid core surrounded by a lipid bilayer containing the membrane (M) (derived from the precursor prM) and envelope (E) proteins. (microbiologyresearch.org)
Genes2
- Expression of cloned envelope protein genes from the flavivirus tick-borne encephalitis virus in mammalian cells and random mutagenesis by PCR. (microbiologyresearch.org)
- The number of children with single nucleotide polymorphisms of P. falciparum histidine-rich protein 2 and 3 genes. (who.int)
Molecular2
- This fact demonstrates that the His-mediated, proton sponge-based endosomal escape saturates at moderate amounts of internalized protein, a fact that might be critical for the design of protein materials for cytosolic molecular delivery. (upc.edu)
- The research themes rank around molecular mechanisms matrix proteins and fibers exert in disorders affecting blood vessels, bone, skin and adipose tissue. (mcgill.ca)
Concomitant1
- Our hypothesis is that this effect is of electrostatic origin and that the observed behavior is due to proton charge fluctuations of the histidines, promoting attractive electrostatic interactions between the positively charged proteins and the anionic surfaces, with concomitant counterion release. (lu.se)
Whey protein8
- ALLWHEY delivers a clean, complete and whole protein that has been sourced from 100% whey protein with zero aminos added. (vitacost.com)
- ALLWHEY has non -denatured intact whey protein fractions that further support protein absorption and utilization. (vitacost.com)
- Hammer's Whey Protein is super pure-free of lactose, fat, and added sugar, and is derived from US-raised, grass-fed, hormone-, and antibiotic-free cows. (hammernutrition.com)
- What is the difference between Whey Protein and Recoverite and what is the best time to use either or both of these products? (hammernutrition.com)
- Lastly, a serving of Whey Protein taken any time during the day is an excellent way to bolster your daily protein intake, helping you reach your optimal amount. (hammernutrition.com)
- Recoverite (but not Whey Protein) also contains the trace mineral chromium polynicotinate (ChromeMate®), which maximizes insulin functioning (very important in the glycogen restoration process), and l-carnosine, which is a powerful antioxidant. (hammernutrition.com)
- In reviewing the Whey Protein product, the protein per serving is 15 grams, which seems low in comparison to other protein products. (hammernutrition.com)
- While it is true that whey protein is popular with bodybuilders and athletes, these are not the only people who can benefit from supplementing with whey. (puritan.com)
Mutation1
- The histidine residue is the most common mutation site in Wilson disease, and this histidine residue is essential for the function of the Menkes ATPase, ATP7A. (medscape.com)
Morphogenesis1
- The results of this study pinpoint a critical residue in the M protein that potentially plays a role in viral morphogenesis, secretion and entry. (microbiologyresearch.org)
Residue1
- In this study, the role of the His residue at position 39 in the M protein (M39) of dengue virus type 2 (DENV-2) in the virus life cycle was investigated. (microbiologyresearch.org)
Phenotype1
- HRP-II (Mr 60,000-105,000) was identified in all P. falciparum parasites regardless of knob-phenotype, and was recovered from culture supernatants as a secreted water-soluble protein. (ox.ac.uk)
Solubility1
- Both of these tags improve fusion protein solubility and expression levels but exhibit immunogenicity for purposes of raising antiprotein antibodies. (genengnews.com)
Polyhistidine3
- Affinity tagging, a popular method for capturing or immobilizing proteins, has several incarnations, none more popular than polyhistidine tagging. (genengnews.com)
- NTA and IDA fix transition metal ions to the matrix, where they complex polyhistidine sequences on proteins of interest. (genengnews.com)
- Cleavage of polyhistidine from the protein is normally not attempted unless the protein is used as a drug in humans or animals. (genengnews.com)
Typically2
- Histidinemia typically causes no health problems, and most people with elevated histidine levels are unaware that they have this condition. (medlineplus.gov)
- Six to eight histidines are typically used to increase specificity over intrinsic histidine in contaminating proteins. (genengnews.com)
Receptors1
- Fig. 1: Photochemistry of light-oxygen-voltage receptors and sequences of proteins under study. (nature.com)
Sequences1
- Assignment of Homology to Genome Sequences using a Library of Hidden Markov Models that Represent all Proteins of Known Structure. (cam.ac.uk)
MRNA2
- Pretreatment of murine myoblast (C2C12) cells with octyl-D-carnosine or carnosine enhanced HIF-1α protein expression, VEGF mRNA levels and VEGF release under hypoxic conditions. (frontiersin.org)
- Hardman et al found that insulin and estrogen increased the level of MNK mRNA and protein levels in the placenta. (medscape.com)
Serving2
- Protein per serving seems low in comparison to other protein products. (hammernutrition.com)
- Each serving provides 250mg of essential Choline and 20g of plant-based protein to help support the increased nutrient demands during pregnancy and lactation. (ritual.com)
Escherichia2
- We also observed that the amount of protein pHis in Escherichia coli lysates depends on carbon source and nitrogen availability in the growth medium. (princeton.edu)
- An impediment to the successful production of large quantities of natively folded proteins in Escherichia coli is the tendency of many proteins to become insoluble when overexpressed. (iucr.org)
Antibody2
- We expect this antibody to open opportunities for investigating other pHis proteins and their functions. (princeton.edu)
- Antibody reactivity against recominant protein and cell lysate for WB. (novusbio.com)
Bind1
- It is also possible to capture the entire population of proteins in cells that bind to metals such as copper or iron, the so-called metalloproteome," adds Dr. Boronowsky. (genengnews.com)
Zinc2
- Zinc-binding proteins such as zinc finger proteins can be captured by zinc-bound resins. (genengnews.com)
- ZIP4 is a member of the Zrt-/Irt-like protein (ZIP) family, and it is a histidine-rich transmembrane protein that is specifically expressed on the apical side of enterocytes in the small intestine, as well as in the kidney, and is responsible for zinc absorption. (medscape.com)
Affinity3
- After expression, the protein is captured by means of immobilized metal affinity chromatography (IMAC). (genengnews.com)
- Histidine tagging is by no means the only affinity tagging option. (genengnews.com)
- The results from our computational analysis demonstrated that the drug-likeness, ADMET, and protein-ligand binding affinity of dasatinib are verified successfully. (bvsalud.org)
Eukaryotic1
- These data indicate that this eukaryotic cell-free expression system has a high success rate and that the addition of specific reagents can increase the yield of soluble protein. (iucr.org)
Concentrations2
- In summary, if the higher concentrations of the protein are toxic or tend to be aggregated inside cells, higher ODs, lower IPTG concentrations, and shorter expression times can be effective in formation of inclusion bodies. (magiran.com)
- High (millimolar) concentrations of the histidine containing dipeptide - carnosine (β-alanine-L-histidine) are present in the skeletal muscle. (frontiersin.org)
Grams of protein1
- While a meal with 20 grams of protein may maximally stimulate muscle building when we are in our 30s, this same amount may stimulate muscle building to a much smaller degree in a more mature adult. (puritan.com)
Lactose1
- ideal for individuals sensitive to lactose in other protein drinks. (vitacost.com)
Soluble2
- In contrast, the expression of soluble proteins needs to be optimized to reduce the amount of accumulated proteins. (magiran.com)
- Inducer concentration, time, and temperature of induction are the main variable in the level of soluble protein or inclusion bodies expression. (magiran.com)
High-protein3
- The partial replacement of semolina with high-protein raw materials leads to the improvement of the biological value of pasta proteins. (mdpi.com)
- Blend, Mix or Shake 1 rounded scoop (30g) with 7 oz (~200 ml) of cold water or low-fat milk, depending on desired taste and consistency, anytime you want a premium and satisfying high-protein beverage. (vitacost.com)
- Whey is popular with bodybuilders, people on high protein diets, and anyone looking for a quick, easy-to-use nutritional drink. (puritan.com)
Copper4
- Copper shows low specificity and high yield, whereas cobalt is on the other end of the spectrum, generating extremely pure proteins but lower yield. (genengnews.com)
- [ 2 ] The MNK protein also became localized toward the basolateral membrane and increased copper transport. (medscape.com)
- His conclusion was that the MNK protein delivers copper to the fetus and Wilson disease ATPase returns excess copper to the maternal circulation. (medscape.com)
- Menkes disease can be caused by both copper transport dysfunction and abnormal protein trafficking. (medscape.com)
Yield2
- The optimal concentration of imidazole needed in the sample and buffer to obtain the best purity and yield differs from protein to protein. (sigmaaldrich.com)
- NTA is a tetravalent ligand providing greater specificity but lower yield, while IDA is trivalent-less selective but generally providing more protein. (genengnews.com)
Powder4
- Devoid of any additional ingredients, this all-natural protein powder harnesses the nutritional goodness of peas, an excellent source of bio-available protein. (vitanetonline.com)
- The history of protein powder traces back to the mid-20th century when athletes started supplementing their diet with protein for enhanced performance. (vitanetonline.com)
- Today, protein powder has made its way to kitchen cabinets of not just bodybuilders, but health-conscious individuals as well. (vitanetonline.com)
- NOW Pea Protein in plain Unflavored is more than just a protein powder-it's your gateway to optimal health and wellbeing. (vitanetonline.com)