• enzymes
  • Such chemical dynamics and biochemical reactions of DNA are much more complex than the molecular dynamics of DNA physical interactions with water, ions and proteins/enzymes in living cells. (wikipedia.org)
  • Youvan and his students have also worked in the field of combinatorial mutagenesis which can be used for directed evolution of proteins, such as enzymes. (wikipedia.org)
  • RecA
  • The specific systems studied by him include RecA, RuvA, uracil DNA glycosylase, single stranded DNA binding protein, ribosome recycling factor, peptidyl tRNA hydrolase, pantothenate kinase and DNA binding protein in stationary phase cells. (wikipedia.org)
  • Crystallography
  • To achieve this goal, the Crystallography and Protein Engineering Unit provides services at different levels in order to meet the demands of research groups at the CNIO and outside our institute. (cnio.es)
  • At the structural determination level, the Unit offers state-of-theart, high-throughput protein crystallisation screening facilities that include sophisticated equipment for the identification of protein crystals, as well as a full-service offering of X-ray crystallography and small-angle x-ray (SAXS) analyses. (cnio.es)
  • More constrained versions of loop modeling are also used in the data fitting stages of solving a protein structure by X-ray crystallography, because loops can correspond to regions of low electron density and are therefore difficult to resolve. (wikipedia.org)
  • receptor
  • These antagonists are engineered for high affinity to the IL-2 receptor a subunit and low affinity to either the [beta] or [gamma] subunit, resulting in a signaling-deficient IL-2 analogue that sequesters the IL-2 receptor a subunit from wild type IL-2. (mit.edu)
  • In the first agent, the double stranded RNA binding domain (dsRBD) of human protein kinase R is used as an siRNA carrier, in fusion proteins that target epidermal growth factor receptor (EGFR). (mit.edu)
  • The nociceptive activity results from cathepsin S functioning as a signaling molecule via activation of protease-activated receptors 2 and 4 members of the G-protein coupled receptor family. (wikipedia.org)
  • Activation loop Autophosphorylation Ca2+/calmodulin-dependent protein kinase Cell signaling Cyclin-dependent kinase G protein-coupled receptor Nucleoside-diphosphate kinase Phosphatase Phosphatidylinositol phosphate kinases Phospholipid Phosphoprotein Phosphorylation Phosphotransferase Signal transduction Thymidine kinase Thymidine kinase in clinical chemistry Thymidylate kinase Wall-Associated Kinase Walker JE, Saraste M, Runswick MJ, Gay NJ (1982). (wikipedia.org)
  • It is a type of α-neurotoxin, a neurotoxic protein that is known to bind competitively and in a relatively irreversible manner to the nicotinic acetylcholine receptor found at the neuromuscular junction, causing paralysis, respiratory failure, and death in the victim. (wikipedia.org)
  • modulate
  • To modulate these protein properties, we utilize the UltraVector ® platform's modular design method to separate proteins into functional elements. (dna.com)
  • According to a 2012 article, Complix has had a degree of success in doing so: "These results show that Alphabodies can be designed to efficiently enter human cells and bind to targets of interest, allowing them to modulate intracellular protein-to-protein interactions and induce apoptosis in cancer cells. (wikipedia.org)
  • reveal
  • Millions of measurements from 23 people who consumed extra calories every day for a month reveal changes in proteins, metabolites, and gut microbiota that accompany shifts in body mass. (the-scientist.com)
  • sequences
  • These techniques involve alignment of target protein sequences with other related protein sequences. (wikipedia.org)
  • These examples establish SCOPE as a standardized method for the construction of synthetic gene libraries from close or distantly related parental sequences to identify functional novelty among the encoded proteins. (wikipedia.org)
  • interact
  • The domain has been verified to interact with other proteins to form complexes or interact with other strcuutres of the cell such as the cytoskeleton or plasma membrane. (wikipedia.org)
  • Youvan's work on proteins that interact with light is referenced in two Nobel Prize Lectures: by Roger Y. Tsien on GFP and by Diesenhofer and Michel for photosynthetic reaction centers. (wikipedia.org)
  • Gene
  • Targeted dsRBD proteins deliver large amounts of siRNA to endosomal compartments in an EGFR expressing cell line, but efficient gene silencing is limited by endosomal escape. (mit.edu)
  • Cathepsin S is a protein that in humans is encoded by the CTSS gene. (wikipedia.org)
  • DEP Domain Containing Protein 1B also known as XTP1, XTP8,HBV XAg-Transactivated Protein 8, [formerly referred to as BRCCis a human protein encoded by a gene of similar name located on chromosome 5. (wikipedia.org)
  • Hephaestin, also known as HEPH, is a protein which in humans is encoded by the HEPH gene. (wikipedia.org)
  • sla mice harbor a partial deletion mutation of the HEPH gene, resulting in the expression of a hephaestin protein that is truncated by 194 amino acids. (wikipedia.org)
  • target
  • As well, experimental evidence suggests that the DEP domain determines the subcellular target of some GTPase Activating proteins. (wikipedia.org)
  • As mentioned above homology-based methods use a database to align the target protein gap with a known template protein. (wikipedia.org)
  • function
  • This alignment can show which amino acids are conserved between species and are important for the function of the protein. (wikipedia.org)
  • His group's research centres on the chemical understanding and exploitation of biomolecular function (Synthetic Biology, Chemical Biology and Chemical Medicine), with an emphasis on carbohydrates and proteins. (wikipedia.org)
  • In the DEPDC1B protein electronic inference has verified the GTPase activator activity function. (wikipedia.org)
  • Research concerning the domain in other proteins indicates an approximately similar function among the domain in various proteins. (wikipedia.org)
  • Certain thermophilic life-forms exist which can withstand temperatures above this, and have corresponding adaptations to preserve protein function at these temperatures. (wikipedia.org)
  • design
  • It is a young discipline, with much research taking place into the understanding of protein folding and recognition for protein design principles. (wikipedia.org)
  • At the protein production level, we have at our disposal a wide array of instrumentation and technical support for the design and purification of soluble recombinant proteins required in large amounts up to milligram quantities, for structural, biophysical or biochemical characterisation, and also for antibody production. (cnio.es)
  • This property is absent in other materials used in nanotechnology, including proteins, for which protein design is very difficult, and nanoparticles, which lack the capability for specific assembly on their own. (wikipedia.org)
  • mice
  • In mice and flies, the Arc protein forms capsids and carries genetic information. (the-scientist.com)
  • The protein was discovered and identified through the study of mice with sex-linked anemia, or sla mice, in which there is normal mucosal uptake of dietary iron but impaired transport of iron from the intestinal enterocytes into the circulation. (wikipedia.org)
  • homology
  • Hephaestin presents homology with ceruloplasmin, a serum dehydrogenase protein involved in copper detoxification and storage. (wikipedia.org)
  • bind
  • Alphabodies, also known as Cell-Penetrating Alphabodies or CPAB for short, are small 10 kDa proteins engineered to bind to a variety of antigens. (wikipedia.org)
  • Alphabodies are different from many other antibody mimetics in their ability to reach and bind to intracellular protein targets. (wikipedia.org)
  • Residues in the groove between helices A and C can be modified to bind convex targets, while residues on the outside of helix C can be modified to bind concave protein targets. (wikipedia.org)
  • known
  • Walker A motif, also known as the Walker loop or P-loop (phosphate-binding loop) is a motif in proteins that is associated with phosphate binding. (wikipedia.org)
  • The Walker A motif is best known for its presence in ATP- and GTP-binding proteins, and is also found in a variety of proteins with phosphorylated substrates. (wikipedia.org)
  • changes
  • This implies knowing how proteins move, detecting their interacting partners, and comprehending the changes that they undergo. (cnio.es)
  • These can include altered bulk properties of the cell to stabilize all proteins, and specific changes to individual proteins. (wikipedia.org)
  • activity
  • Studies suggest that this truncated hephaestin protein still retains a minimal, yet detectable and quantifiable level of ferroxidase activity. (wikipedia.org)
  • Cell
  • Also, DEPDC1B expression decreases in environments of beta-catenin depletion in multiple myeloma cell lines No interactions of DEPDC1B within any other protein product characterized by experimentation have been verified. (wikipedia.org)
  • Biotechnology
  • Research in the Davis laboratory has been funded by the Engineering and Physical Sciences Research Council (EPSRC), the Biotechnology and Biological Sciences Research Council (BBSRC), the Medical Research Council, UCB-Celltech, AstraZeneca, the European Union, GlaxoSmithKline, Cancer Research UK, the Wellcome Trust and the Royal Society. (wikipedia.org)
  • research
  • It was formed around the research of two prominent scientists who invented protein engineering, Sir Alan Fersht and Sir Greg Winter. (wikipedia.org)
  • After his PhD, Davis spent two years as a postdoctoral research fellow in the laboratory of J. Bryan Jones at the University of Toronto, exploring protein chemistry and biocatalysis.In 1998 he returned to the United Kingdom to take up a lectureship at Durham University. (wikipedia.org)
  • His research in the area has been concerned with mycobacterial, particularly tuberculosis, related proteins. (wikipedia.org)