• RecA
  • The specific systems studied by him include RecA, RuvA, uracil DNA glycosylase, single stranded DNA binding protein, ribosome recycling factor, peptidyl tRNA hydrolase, pantothenate kinase and DNA binding protein in stationary phase cells. (wikipedia.org)
  • sequence
  • While the sequence-conformation space that needs to be searched is large, the most challenging requirement for computational protein design is a fast, yet accurate, energy function that can distinguish optimal sequences from similar suboptimal ones. (wikipedia.org)
  • Without structural information about a protein, sequence analysis is often useful in elucidating information about the protein. (wikipedia.org)
  • Multiple sequence alignment utilizes data bases such as PREFAB, SABMARK, OXBENCH, IRMBASE, and BALIBASE in order to cross reference target protein sequences with known sequences. (wikipedia.org)
  • Intrexon's proprietary database catalogs comprehensive information about the genetic and protein components based on structure-based sequence alignment, de novo and comparative protein modeling, molecular dynamics simulation and free energy analysis. (dna.com)
  • In a 1984 publication with John E. Hearst, and in collaboration with Barry L. Marrs, Youvan published the nucleotide and deduced protein sequence for the photosynthetic reaction center - the proteins that convert light to chemical energy in photosynthetic organisms. (wikipedia.org)
  • While hephaestin shares 50% amino acid sequence identity with its serum homologue ceruloplasmin, the hephaestin protein includes an additional 86 amino acids at the C-terminus, which code for a single transmembrane domain and a short cytoplasmic tail. (wikipedia.org)
  • Crystallography
  • To achieve this goal, the Crystallography and Protein Engineering Unit provides services at different levels in order to meet the demands of research groups at the CNIO and outside our institute. (cnio.es)
  • At the structural determination level, the Unit offers state-of-theart, high-throughput protein crystallisation screening facilities that include sophisticated equipment for the identification of protein crystals, as well as a full-service offering of X-ray crystallography and small-angle x-ray (SAXS) analyses. (cnio.es)
  • More constrained versions of loop modeling are also used in the data fitting stages of solving a protein structure by X-ray crystallography, because loops can correspond to regions of low electron density and are therefore difficult to resolve. (wikipedia.org)
  • receptor
  • These antagonists are engineered for high affinity to the IL-2 receptor a subunit and low affinity to either the [beta] or [gamma] subunit, resulting in a signaling-deficient IL-2 analogue that sequesters the IL-2 receptor a subunit from wild type IL-2. (mit.edu)
  • In the first agent, the double stranded RNA binding domain (dsRBD) of human protein kinase R is used as an siRNA carrier, in fusion proteins that target epidermal growth factor receptor (EGFR). (mit.edu)
  • The nociceptive activity results from cathepsin S functioning as a signaling molecule via activation of protease-activated receptors 2 and 4 members of the G-protein coupled receptor family. (wikipedia.org)
  • Activation loop Autophosphorylation Ca2+/calmodulin-dependent protein kinase Cell signaling Cyclin-dependent kinase G protein-coupled receptor Nucleoside-diphosphate kinase Phosphatase Phosphatidylinositol phosphate kinases Phospholipid Phosphoprotein Phosphorylation Phosphotransferase Signal transduction Thymidine kinase Thymidine kinase in clinical chemistry Thymidylate kinase Wall-Associated Kinase Walker JE, Saraste M, Runswick MJ, Gay NJ (1982). (wikipedia.org)
  • It is a type of α-neurotoxin, a neurotoxic protein that is known to bind competitively and in a relatively irreversible manner to the nicotinic acetylcholine receptor found at the neuromuscular junction, causing paralysis, respiratory failure, and death in the victim. (wikipedia.org)
  • modulate
  • To modulate these protein properties, we utilize the UltraVector ® platform's modular design method to separate proteins into functional elements. (dna.com)
  • According to a 2012 article, Complix has had a degree of success in doing so: "These results show that Alphabodies can be designed to efficiently enter human cells and bind to targets of interest, allowing them to modulate intracellular protein-to-protein interactions and induce apoptosis in cancer cells. (wikipedia.org)
  • reveal
  • Millions of measurements from 23 people who consumed extra calories every day for a month reveal changes in proteins, metabolites, and gut microbiota that accompany shifts in body mass. (the-scientist.com)
  • mutations
  • However, its major drawback is that detailed structural knowledge of a protein is often unavailable, and, even when available, it can be very difficult to predict the effects of various mutations. (wikipedia.org)
  • Nucleic
  • In this field, nucleic acids are used as non-biological engineering materials for nanotechnology rather than as the carriers of genetic information in living cells. (wikipedia.org)
  • sequences
  • These techniques involve alignment of target protein sequences with other related protein sequences. (wikipedia.org)
  • These examples establish SCOPE as a standardized method for the construction of synthetic gene libraries from close or distantly related parental sequences to identify functional novelty among the encoded proteins. (wikipedia.org)
  • interact
  • The domain has been verified to interact with other proteins to form complexes or interact with other strcuutres of the cell such as the cytoskeleton or plasma membrane. (wikipedia.org)
  • Youvan's work on proteins that interact with light is referenced in two Nobel Prize Lectures: by Roger Y. Tsien on GFP and by Diesenhofer and Michel for photosynthetic reaction centers. (wikipedia.org)
  • disease
  • A protein constructed by these researchers has yielded experimental results that are promising when it comes to stopping the disease. (innovations-report.com)
  • One of the strategies for finding an answer to how Alzheimer's disease could be prevented is based on the idea of adding a protein to the blood, to bind to the Aß peptide there. (innovations-report.com)
  • target
  • As well, experimental evidence suggests that the DEP domain determines the subcellular target of some GTPase Activating proteins. (wikipedia.org)
  • As mentioned above homology-based methods use a database to align the target protein gap with a known template protein. (wikipedia.org)
  • bind
  • Alphabodies, also known as Cell-Penetrating Alphabodies or CPAB for short, are small 10 kDa proteins engineered to bind to a variety of antigens. (wikipedia.org)
  • Residues in the groove between helices A and C can be modified to bind convex targets, while residues on the outside of helix C can be modified to bind concave protein targets. (wikipedia.org)
  • function
  • This alignment can show which amino acids are conserved between species and are important for the function of the protein. (wikipedia.org)
  • His group's research centres on the chemical understanding and exploitation of biomolecular function (Synthetic Biology, Chemical Biology and Chemical Medicine), with an emphasis on carbohydrates and proteins. (wikipedia.org)
  • In the DEPDC1B protein electronic inference has verified the GTPase activator activity function. (wikipedia.org)
  • Research concerning the domain in other proteins indicates an approximately similar function among the domain in various proteins. (wikipedia.org)
  • Certain thermophilic life-forms exist which can withstand temperatures above this, and have corresponding adaptations to preserve protein function at these temperatures. (wikipedia.org)
  • design
  • It is a young discipline, with much research taking place into the understanding of protein folding and recognition for protein design principles. (wikipedia.org)
  • This property is absent in other materials used in nanotechnology, including proteins, for which protein design is very difficult, and nanoparticles, which lack the capability for specific assembly on their own. (wikipedia.org)
  • mice
  • In mice and flies, the Arc protein forms capsids and carries genetic information. (the-scientist.com)
  • The protein was discovered and identified through the study of mice with sex-linked anemia, or sla mice, in which there is normal mucosal uptake of dietary iron but impaired transport of iron from the intestinal enterocytes into the circulation. (wikipedia.org)
  • sla mice harbor a partial deletion mutation of the HEPH gene, resulting in the expression of a hephaestin protein that is truncated by 194 amino acids. (wikipedia.org)
  • homology
  • Hephaestin presents homology with ceruloplasmin, a serum dehydrogenase protein involved in copper detoxification and storage. (wikipedia.org)
  • known
  • Walker A motif, also known as the Walker loop or P-loop (phosphate-binding loop) is a motif in proteins that is associated with phosphate binding. (wikipedia.org)
  • The Walker A motif is best known for its presence in ATP- and GTP-binding proteins, and is also found in a variety of proteins with phosphorylated substrates. (wikipedia.org)
  • Hephaestin, also known as HEPH, is a protein which in humans is encoded by the HEPH gene. (wikipedia.org)
  • changes
  • This implies knowing how proteins move, detecting their interacting partners, and comprehending the changes that they undergo. (cnio.es)
  • activity
  • Studies suggest that this truncated hephaestin protein still retains a minimal, yet detectable and quantifiable level of ferroxidase activity. (wikipedia.org)
  • expression
  • Expression and processing of proteins in different prokaryotic and eukaryotic cell systems, for innovative hemibodies with defined characteristics. (academics.de)
  • Also, DEPDC1B expression decreases in environments of beta-catenin depletion in multiple myeloma cell lines No interactions of DEPDC1B within any other protein product characterized by experimentation have been verified. (wikipedia.org)
  • Research
  • The research team has studied how an artificial so-called Affibody protein entirely encases the Aß peptide, thereby preventing the formation of toxic forms. (innovations-report.com)
  • It was formed around the research of two prominent scientists who invented protein engineering, Sir Alan Fersht and Sir Greg Winter. (wikipedia.org)
  • After his PhD, Davis spent two years as a postdoctoral research fellow in the laboratory of J. Bryan Jones at the University of Toronto, exploring protein chemistry and biocatalysis.In 1998 he returned to the United Kingdom to take up a lectureship at Durham University. (wikipedia.org)
  • Research in the Davis laboratory has been funded by the Engineering and Physical Sciences Research Council (EPSRC), the Biotechnology and Biological Sciences Research Council (BBSRC), the Medical Research Council, UCB-Celltech, AstraZeneca, the European Union, GlaxoSmithKline, Cancer Research UK, the Wellcome Trust and the Royal Society. (wikipedia.org)
  • His research in the area has been concerned with mycobacterial, particularly tuberculosis, related proteins. (wikipedia.org)