• yeast
  • Recent emergence of oseltamivir and zanamivir resistant human influenza A(H1N1) H274Y has emphasized the need for suitable expression systems to obtain large quantities of highly pure and stable, recombinant neuraminidase through two separate artificial tetramerization domains that facilitate the formation of catalytically active neuraminidase homotetramers from yeast and Staphylothermus marinus, which allow for secretion of FLAG-tagged proteins and further purification. (wikipedia.org)
  • nucleus
  • Most importins, like the importin a1, tow upwards of 500-600 different proteins into the nucleus. (jefferson.edu)
  • Where it is expressed, the protein is predicted to be localized in the nucleus to carry out a function. (wikipedia.org)
  • Several possible phosphorylation sites were identified along with the associated kinases: UPF0575 protein C19orf67 is expected to be targeted in the nucleus, specifically the nucleolus. (wikipedia.org)
  • vitro
  • It should then be expected that T-cell recognition of protein antigens in vitro will be independent of protein conformation. (biochemj.org)
  • Three T-cell lines were made by passage in vitro with native lysozyme of T cells from two mouse strains (B10.BR and DBA/1) that had been primed with the same protein. (biochemj.org)
  • scrapie
  • The protein can exist in multiple isoforms, the normal PrPC, and as Protease resistant PrPRes like the disease-causing PrPSc(scrapie) and an isoform located in mitochondria. (wikipedia.org)
  • molecules
  • Tanaka, M., Machida, Y., and Nukina, N. A novel therapeutic strategy for polyglutamine diseases by stabilizing aggregation-prone proteins with small molecules. (riken.jp)
  • Gow and Lazzarini, 1996 ), but precisely how these molecules may interact and how they may influence the conformation of one another is at present unknown. (jneurosci.org)
  • Levitt was a PhD student in Computational biology at Peterhouse, Cambridge, and was based at the Laboratory of Molecular Biology from 1968 to 1972, where he developed a computer program for studying the conformations of molecules that underpinned much of his later work. (wikipedia.org)
  • abundant
  • The proline content by percentage was found to be higher in UPF0575 than 95% of comparable human proteins.However, the amount of asparagine the protein is less abundant when compared to the human proteome. (wikipedia.org)
  • The protein product is less abundant than most of the human proteome in many tissues. (wikipedia.org)
  • Alpha-synuclein is a protein that is abundant in the human brain. (wikipedia.org)
  • interaction
  • Fluorinated C3-benzamido substituents induced a shift in the side-chain conformation of R144 to allow for an entropically favored electrostatic interaction between its guanidine group and the 2- O -sulfate of the ligand. (rsc.org)
  • Together, RCC1's shape and importin a3's ability to change its conformation drive this selective interaction. (jefferson.edu)
  • sequence
  • 44 prolines were found along the protein, making up 12.3% of the total amino acid sequence. (wikipedia.org)
  • A cross-program consensus predicted that UPF0575 protein C19orf67 forms four alpha helices and two beta sheets in the following locations in the amino acid sequence: Acetylation is likely to occur at the N-terminus while the C-terminus is unlikely to be modified. (wikipedia.org)
  • Mitchison, Graeme (1998), Biological Sequence Analysis: Probabilistic Models of Proteins and Nucleic Acids (1st ed. (wikipedia.org)
  • multitude
  • In this study, Dr. Cingolani's "cargo" was the RCC1 protein, which has a multitude of functions in the cell, and without which, the cell dies. (jefferson.edu)
  • Disease
  • Neame, P. J. and Gallagher, J. T. (2005) Extracellular Matrix Heparan Sulfate Proteoglycans, in Amyloid Proteins: The Beta Sheet Conformation and Disease (ed J. D. Sipe), Wiley-VCH Verlag GmbH, Weinheim, Germany. (wiley.com)
  • An alpha-synuclein fragment, known as the non-Abeta component (NAC) of Alzheimer's disease amyloid, originally found in an amyloid-enriched fraction, was shown to be a fragment of its precursor protein, NACP. (wikipedia.org)
  • Atoms
  • Experimental techniques are able to estimate distances between pairs of atoms of a given molecule, and the problem becomes the one of identifying the three-dimensional conformation of the molecule, i.e. the positions of all its atoms. (wikipedia.org)
  • function
  • The highly conserved and slowly evolving DUFF3314 region is predicted to form numerous alpha helices and may be vital to the function of the protein. (wikipedia.org)
  • Although the function is not yet well understood, conservation among numerous taxa indicate that it may be important to the function of the protein. (wikipedia.org)
  • Alpha-synuclein is a synuclein protein of unknown function primarily found in neural tissue, making up as much as 1% of all proteins in the cytosol of brain cells. (wikipedia.org)
  • In this field, the main interest is on proteins, because discovering their three-dimensional conformation allows us to get clues about the function they are able to perform. (wikipedia.org)
  • gene
  • The spliceosome: NMR spFRET dice up protein complex spectroscopyNOW.com Cells convert DNA to RNA to template protein production but before that final step, gene expression, the non-coding regions of the genome have to be removed by the so-called spliceosome. (bionmr.com)
  • UPF0575 protein C19orf67 is a protein which in humans (Homo sapiens) is encoded by the C19orf67 gene. (wikipedia.org)
  • The DUF3314 region of the gene appears to have diverged at a slower rate relative to the rest of the gene, indicating that that region may have been undergoing purifying selection because that region played an important role in the protein. (wikipedia.org)
  • 1993
  • From 1993 to 1995, Clemmer was a Postdoctoral Research Associate at Northwestern University, where he worked with Martin F. Jarrold, studying protein folding and protein conformation in the gas phase, using techniques such as Ion-mobility spectrometry. (wikipedia.org)
  • Cells
  • Presentation of a protein antigen to T cells is believed to follow its intracellular breakdown by the antigen-presenting cell, with the fragments constituting the trigger of immune recognition. (biochemj.org)
  • To perform key tasks, cells use molecular carriers to help shuttle a diverse array of specialized proteins - like a car or truck pulling a cargo trailer - into their nuclei. (jefferson.edu)
  • It is predominantly a neuronal protein, but can also be found in the neuroglial cells. (wikipedia.org)
  • citation needed] In melanocytic cells, SNCA protein expression may be regulated by MITF. (wikipedia.org)
  • highly
  • Thus, the study of proteins in the gas-phase on this time-scale is highly desirable. (pubmedcentralcanada.ca)
  • In humans, UPF0575 protein C19orf67 is highly expressed in the testis and breast tissue, although it is also expressed at low levels in the stomach, cerebral cortex, thyroid gland, and salivary gland. (wikipedia.org)
  • However
  • However both protein unfolding and superactivity is reversed back to a native-like condition with UV illumination. (usc.edu)
  • helix
  • Other helices, such as the 310 helix and π helix, are calculated to have energetically favorable hydrogen-bonding patterns but are rarely observed in natural proteins except at the ends of α helices due to unfavorable backbone packing in the center of the helix. (wikipedia.org)