• Fission yeast is a single-celled organism with chromosomes very similar to those in humans, and uses the same genes to maintain those chromosomes. (usc.edu)
  • KAT11 and H3K56 acetylation appear to correlate with actively transcribed genes and associate with the elongating form of Pol II in yeast (PMID:17046836). (embl.de)
  • The phylogenetic position of Schizosaccharomyces species has also been controversial: do they belong to Taphrinomycotina (previously known as archiascomycetes) as predicted by analyses with nuclear genes, or are they instead related to Saccharomycotina (budding yeast) as in mitochondrial phylogenies? (umontreal.ca)
  • bimA is a member of a gene family defined by a repeated motif called the Tetratrico Peptide Repeat (TPR), which is found in genes from bacteria, yeast and insects. (rupress.org)
  • Among CRE1 repressed transcripts, genes encoding unknown proteins and transport proteins were overrepresented. (biomedcentral.com)
  • In the genome of the grey mould fungus Botrytis cinerea , genes encoding three hydrophobins and six hydrophobin-like proteins were identified. (biomedcentral.com)
  • This domain occurred 388 times on human genes ( 899 proteins). (umbc.edu)
  • The CST complex is a phylogenetically conserved protein complex consisting of CTC1/Cdc13, Stn1 and Ten1 that protects telomeres on linear chromosomes. (nih.gov)
  • While a postdoctoral student at MIT, Tomomi Kiyomitsu discovered how dynein has a role as a motor protein in aligning the chromosomes in the middle of the cell during the metaphase of mitosis. (wikipedia.org)
  • Fission yeast is a simple, single-celled organism with chromosomes very similar to those in humans, and has proven to be an important model for cell division. (usc.edu)
  • Although hybrids of these two species inherit three chromosomes from each parent, the majority of spores (the yeast equivalent of sperm) that these hybrids produce fail to develop into new yeast cells. (elifesciences.org)
  • Kinetochores are protein complexes on the chromosomes, whose function as linkers between spindle microtubules and chromosomes is crucial for proper cell division. (nature.com)
  • An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis. (thebiogrid.org)
  • Here we identify causes underlying hybrid infertility of two recently diverged fission yeast species Schizosaccharomyces pombe and S. kambucha , which mate to form viable hybrid diploids that efficiently complete meiosis, but generate few viable gametes. (elifesciences.org)
  • have now looked at two species of fission yeast- S. pombe and S. kambucha -that share 99.5% of their DNA sequence. (elifesciences.org)
  • Since these two yeast species are so closely related, the findings of Zanders et al. (elifesciences.org)
  • In more distant species, putative homologues were detected for Ykl155c, which shares conserved motifs with uncharacterized proteins of higher eukaryotes including humans. (pasteur.fr)
  • In an effort to understand the fundamental signaling elements, or 'core machinery', required for the function of these GTPases, we describe here the conservation and functional similarities of Cdc42 and Rac effectors in five different species: plant, yeast, fruit fly, roundworm and human. (biomedcentral.com)
  • In addition, they play important roles in gene transcription (via activation of mitogen activated protein (MAP) kinase pathways and, in higher eukaryotes, the transcription factor NFκB), generation of reactive oxygen species, apoptosis, and cell-cycle progression. (biomedcentral.com)
  • We first aligned and catalogued the families of SH3-containing proteins in these four species to determine the relationships between homologous domains. (upf.edu)
  • All SPOT readouts were hierarchically clustered and we observed that the organization of the SH3 specificity landscape in three distinct profile classes remains conserved across these four yeast species. (upf.edu)
  • Modifications to techniques commonly used in related species (e.g., budding yeast) are noted, as are useful resources for fission yeast researchers, including various databases and repositories. (cshlpress.com)
  • The genome sequence of Schizosaccharomyces pombe. (genome.jp)
  • Mitochondrial TAT transports folded proteins in those eukaryotes with TatA and TatC subunits encoded in the mitochondrial genome. (tcdb.org)
  • Database is richly annotated in the systemic information on the nomenclature, chemistry and structure of the DNA damage and drugs targeting nucleic acids and/or proteins involved in the maintenance of genome stability. (vifabio.de)
  • Accordingly, age-related DNA damage and genome instability were lower in fat body of the mutant, and overexpression of a minichromosome maintenance protein subunit extended lifespan. (bvsalud.org)
  • Of course unlike even the most grizzled 30 year coffee drinker with ideal genetics, the yeast almost certainly gets no joy from its morning Joe. (yeastgenome.org)
  • ANI-1 and ANI-2 (proteins homologous to anillin) are essential for embryonic viability in both organisms. (wikipedia.org)
  • Here we report that the non-homologous end-joining (NHEJ) protein Ku becomes critical for survival of replication stress in brc1∆ cells. (thebiogrid.org)
  • We have cloned the RAD6 homolog from Drosophila melanogaster and find that its encoded protein displays a very high degree of identity in amino acid sequence with the homologous RAD6 proteins from the two divergent yeasts, S. cerevisiae and Schizosaccharomyces pombe, and from human. (eur.nl)
  • In addition, we show that position-weighted matrices (PWM) compiled from SPOT assays can be used for binding motif screening in potential binding partners and present cases where motifs are either conserved or lost among homologous SH3 interacting proteins. (upf.edu)
  • When Wang and coworkers added the gene to S. cerevisiae , this newly engineered yeast could now better tolerate caffeine. (yeastgenome.org)
  • Unlike the two S. cerevisiae Pif1 subfamily proteins, the S. pombe Pfh1p was essential. (princeton.edu)
  • Mitochondrial ribosomal proteins were studied best in yeast, where the small subunit was shown to contain about 35 proteins. (pasteur.fr)
  • Yet, genetic and biochemical studies identified only 14 proteins, half of which were predictable by sequence homology with prokaryotic ribosomal components of the small subunit. (pasteur.fr)
  • Using a recently described affinity purification technique and tagged versions of yeast Ykl155c and Mrp1, we isolated this mitochondrial ribosomal subunit and identified a total of 20 proteins, of which 12 are new. (pasteur.fr)
  • This brings to 26 the total number of proteins described as components of the mitochondrial small ribosomal subunit. (pasteur.fr)
  • Mitochondria import nuclearly-encoded proteins, made in the cell cytoplasm, into the mitochondrial matrix where their mitochondrial targeting sequences are removed by proteolysis. (tcdb.org)
  • The matrix exposed C-terminal α-helical domain of Oxa1 can bind mitochondrial ribosomes to facilitate co-translational insertion of proteins into the mitochondrial membrane ( Jia et al . (tcdb.org)
  • Remarkably, almost half of the previously and newly identified mitochondrial ribosomal components showed no similarity to any known ribosomal protein. (pasteur.fr)
  • Another newly identified ribosomal protein, Ygl129c, was previously shown to be a member of the DAP-3 family of mitochondrial apoptosis mediators. (pasteur.fr)
  • Glutaminase liver isoform, mitochondrial is a protein in humans that is encoded by GLS2 gene. (cusabio.com)
  • Schizosaccharomyces pombe Fe-sulfur cluster assembly protein Isu1 (isu1), mRNA. (genscript.com)
  • The high degree of structural and functional conservation of RAD6 in eukaryotic evolution suggests that the various protein components involved in RAD6-dependent DNA repair and mutagenesis functions have also been conserved. (eur.nl)
  • Here we probe these fundamental questions using peroxisomal compartmentalization of the last steps of lysine and histidine biosynthesis in the fission yeast Schizosaccharomyces japonicus . (biorxiv.org)
  • The fission yeast Schizosaccharomyces japonicus provides an attractive stripped-down model for metabolic compartmentalization in peroxisomes. (biorxiv.org)
  • The human anillin cDNA, located on Chr7, encodes a 1,125-amino acid protein with a predicted molecular mass of 124 kD and a pI of 8.1. (wikipedia.org)
  • In a new study in the Journal of Agricultural and Food Chemistry , Wang and coworkers were able to toughen up budding yeast against caffeine by adding bfr1 , a gene from Schizosaccharomyces pombe that encodes the ABC transporter that shunts caffeine out of the cell. (yeastgenome.org)
  • Here we describe the isolation and characterization of pfh1 + , a Schizosaccharomyces pombe gene that encodes a Pif1-like protein. (princeton.edu)
  • Here we describe the isolation and characterization of pfh1+, a Schizosaccharomyces pombe gene that encodes a Pif1-like protein. (princeton.edu)
  • All of our recombinant proteins are manufactured in strictly controlled facilities and by using a well established technology which guarantees full batch-to-bact consistency and experiment reproducibility. (arrestins.com)
  • The C-terminal domain of the 990-amino acid Schizosaccharomyces pombe Spt5 protein, composed of repeats of a nonapeptide motif (consensus sequence TPAWNSGSK), is necessary and sufficient for binding to the capping enzymes in vivo (in a two-hybrid assay) and in vitro. (mskcc.org)
  • When they looked at the mutant, Wang and coworkers found that there were 11 amino acid substitutions scattered across the protein. (yeastgenome.org)
  • First identified in Drosophila, the conserved Polycomb group (Pc-G) protein complexes were shown to be both writers (Polycomb Repressive Complex 2, PRC2) and readers (Polycomb Repressive Complex 1, PRC1) of H3K27me3 [ 19 , 20 , 21 ]. (biomedcentral.com)
  • The chloroplast albino 3 (ALB3) protein appears to integrate the light harvesting chlorophyll-binding protein into thylakoid membranes using a pathway that is distinct from the chloroplast Sec translocation pathway. (tcdb.org)
  • Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. (thebiogrid.org)
  • In lower multicellular fungi, the C2H2 zinc finger CreA/CRE1 protein has been shown to act as the transcriptional repressor in this process. (biomedcentral.com)
  • Here, we performed tissue-specific transcriptional and proteomic profiling of long-lived Drosophila dilp2-3,5 mutants, and identified tissue-specific regulation of >3600 transcripts and >3700 proteins. (bvsalud.org)
  • These sequences represent the protein coding region of the isu1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. (genscript.com)
  • Bacteria also export (to the periplasm) N-tails of membrane proteins synthesized without leader sequences by a Sec (Type IIPS)-independent mechanism. (tcdb.org)
  • Homologues could be found, however, in predicted protein sequences from Schizosaccharomyces pombe. (pasteur.fr)
  • There are also numerous anillin-like protein homologues found outside of metazoans. (wikipedia.org)
  • Homologues of the yeast Oxa1 protein are found in chloroplasts of plants and in a wide variety of bacteria. (tcdb.org)
  • Yeast encode two distant YidC homologues, Oxa1 and Oxa2. (tcdb.org)
  • We have recently shown that MTs explore the space by pivoting around the SPB, which accelerates KC capture in mitosis in fission yeast 9 . (nature.com)
  • BIMA, a TPR-containing protein required for mitosis, localizes to the spindle pole body in Aspergillus nidulans. (rupress.org)
  • La position phylogénétique du genre Schizosaccharomyces est aussi controversée: appartient-il aux Taphrinomycotina (précédemment connus comme archiascomycetes) comme prédit par l'analyse de gènes nucléaires, ou est-il plutôt relié aux Saccharomycotina (levures bourgeonnantes) tel que le suggère la phylogénie mitochondriale? (umontreal.ca)
  • Dans le Chapitre 3, nous montrons à l'aide d'un jeu de données nucléaires important (plus de cent protéines) et avec supports statistiques concluants, que le genre Schizosaccharomyces appartient aux Taphrinomycotina. (umontreal.ca)
  • Cdc42 and/or Rac homologs are found in fruit flies, round-worms and budding yeast. (biomedcentral.com)
  • We report the adaptation to Schizosaccharomyces pombe of the anchor-away technique, originally designed in budding yeast by Laemmli lab. (tau.ac.il)
  • PBS2 and Wis1 are components of stress-activated MAPK cascades in budding and fission yeast, respectively. (umbc.edu)
  • PBS2 is the specific activator of the MAPK Hog1, which plays a central role in the response of budding yeast to stress including exposure to arsenite and hyperosmotic environments. (umbc.edu)
  • The proteasome is the main cellular proteolytic system and plays a fundamental role in the maintenance of protein homeostasis. (bvsalud.org)
  • Protein kinases (PKs), MAP kinase kinase (MAPKK) subfamily, fungal PBS2-like proteins, catalytic (c) domain. (umbc.edu)
  • The MAPKK subfamily is part of a larger superfamily that includes the catalytic domains of other protein serine/threonine kinases, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase. (umbc.edu)
  • Here, we isolate a previously uncharacterized S. pombe protein through association with the Cdc14 phosphatase homologue, Clp1/Flp1, and identify it as a Borealin-like member of the CPC. (uky.edu)
  • The contributors describe basic methods for culturing and genetically manipulating fission yeast, synchronization strategies for probing the cell cycle, technologies for assessing proteins, metabolites, and cell wall constituents, imaging methods to visualize subcellular structures and dynamics, and protocols for investigating chromatin and nucleic acid metabolism. (cshlpress.com)
  • Proteins involved in carbohydrate metabolism showed altered expression in the mutant intestine, and gut-specific overexpression of a lysosomal mannosidase increased autophagy, gut homeostasis, and lifespan. (bvsalud.org)
  • Mitotic regulation of protein phosphatases by the fission yeast sds22 protein. (anticorps-enligne.fr)
  • This analysis revealed a striking example of binding motif divergence in a C. albicans Rvs167 paralog, which cannot be explained by overall SH3 sequence or interface residue divergence, and we validated this specificity change with a yeast two-hybrid (Y2H) assay. (upf.edu)
  • Although dozens of proteins act downstream of these GTPases, a comparison of effector proteins from evolutionarily diverse organisms suggests that six groups of proteins serve as the core machinery for signaling from Cdc42 and Rac. (biomedcentral.com)
  • The analysis of Cdc42 and Rac function in evolutionarily distant organisms is useful as a tool to uncover the basic activities of these proteins. (biomedcentral.com)
  • By regulating filamentous actin, Cdc42 and Rac exert a profound effect on cell shape, polarity, migration, cell:cell and cell:matrix adhesion, protein traffic, and cytokinesis. (biomedcentral.com)
  • In mammalian cells, as many as twenty types of protein have been reported to bind to activated Cdc42 [ 2 ]. (biomedcentral.com)
  • The mitogen-activated protein (MAP) kinase signaling pathways are important mediators of cellular responses to extracellular signals. (umbc.edu)
  • The latter can replace E. coli YidC for Sec-independent insertion of proteins ( Bloois et al . (tcdb.org)
  • Arrestins (abbreviated Arr) are a family of proteins which are important in the regulation of signal transduction G-protein coupled receptors, which at first was discovered, as part of a conserved two step mechanism for regulating the activity of G protein-coupled receptors (GPCR) in rhodopsin visual system by Ursula Kuhn found Scott court, and β-called Global Martin J. Lohse system and kidney and colleagues. (arrestins.com)
  • Collectively, we propose that fission yeast Stn1 primarily safeguards the semi-conservative DNA replication at telomeres and subtelomeres. (nih.gov)
  • Many viruses use the microtubule transport system to transport nucleic acid/protein cores to intracellular replication sites after invasion host the cell membrane. (wikipedia.org)
  • and the contribution of chromatin-modifying proteins to replication and repair. (usc.edu)
  • The papers presented covered a wide range of innovative topics for the wine industry, such as efficient water management for vineyard irrigation, the application of mechanical selective harvesting, a new digital tool for training in vine pruning, the use of yeast derivatives in winemaking, new methods for wine stabilisation, oxidative and aromatic stability of wines, and many others. (infowine.com)
  • An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. (thebiogrid.org)
  • 2018 ). Through YidC, proteins are inserted into the lipid bilayer via the SecYEG-dependent complex, but YidC functions as a chaperone in protein folding processes. (tcdb.org)
  • Dyneins are a family of cytoskeletal motor proteins that move along microtubules in cells . (wikipedia.org)
  • For a subset of the newly described ribosomal proteins, we showed that they are localized in mitochondria and are required for the respiratory competency of the yeast cells. (pasteur.fr)
  • Thestructure reveals a buried autoacetylated lysine residue that we show is alsoacetylated in the Rtt109 protein purified from yeast cells. (embl.de)
  • While not as robust as bfr1-B , this mutant allowed yeast expressing it to tolerate caffeine concentrations up to 15 mg/ml, conditions under which cells with wild type bfr1 failed to grow. (yeastgenome.org)
  • Anillin is a conserved protein implicated in cytoskeletal dynamics during cellularization and cytokinesis. (wikipedia.org)
  • Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. (thebiogrid.org)
  • Studies using fission yeast were instrumental in identifying fundamental mechanisms that govern cell division, differentiation, and epigenetics, to name but a few. (cshlpress.com)
  • During this highly polarized growth, micro-tubules are responsible for the placement of the cell-end marker proteins, the Teal-Tea4/Wsh3 complex, which recruits the Pom1 DYRK-family protein kinase. (escholarship.org)
  • Thus, this study characterizes a fission yeast Borealin homologue and reveals a previously unrecognized connection between the CPC and the process of cytokinesis in S. pombe. (uky.edu)
  • This method relies on a rapamycin-mediated interaction between the FRB- and FKBP12-binding domains to relocalize nuclear proteins of interest to the cytoplasm. (tau.ac.il)
  • abstract = "Genetic analysis of protein function requires a rapid means of inactivating the gene under study. (tau.ac.il)
  • Fission yeast are unicellular, rod-shaped fungi that divide by medial fission. (cshlpress.com)
  • Hydrophobins are small, cysteine rich, surface active proteins secreted by filamentous fungi, forming hydrophobic layers on the walls of aerial mycelia and spores. (biomedcentral.com)
  • Filamentous fungi produce unique proteins called hydrophobins that are secreted and cover the walls of spores and hyphae with a hydrophobic layer [ 1 ]. (biomedcentral.com)