• junctional
  • Several laboratories have demonstrated a decrease in gap junctional communication in cells transformed by the src oncogene of the Rous sarcoma virus. (hawaii.edu)
  • Since the presence of phosphotyrosine on Cx43 is correlated with the down regulation of gap-junctional communication, these results suggest that pp60v-src regulates gap junctional gating activity via tyrosine phosphorylation of Cx43. (hawaii.edu)
  • In addition to making gap junctions, innexins also form non-junctional membrane channels with properties similar to those of pannexons. (wikipedia.org)
  • sequence homology
  • Overall sequence homology with Cx32 and Cx43 (64 and 51% amino acid identities, respectively) and a similar predicted tertiary structure confirm that this protein forms part of the connexin family and is consequently referred to as Cx26. (rupress.org)
  • consist
  • Excretome/secretome (ES) components essentially consist of proteins and other compounds that are secreted from the parasite and it constitutes the primary interface between parasite and host and may be further exploited as potential vaccine candidates. (wikipedia.org)
  • mutations
  • The majority of these mutations only change a single amino acid within the protein chain, which result in a different protein being produced. (wikipedia.org)
  • These mutations most commonly result in proteins that work incorrectly, less effectively, degrade faster, are not present in adequate numbers or may not function at all. (wikipedia.org)
  • connexon
  • Gap junction proteins of connexin (Cx) family were examined by using specific rabbit antibodies raised against type-specific peptide sequence domains at the single connexon channel level and on the tissue distiribution and cell type secificity. (nii.ac.jp)
  • 1. Single gap junction channel (connexon) particles isolated from rat and guinea pig liver by mild detergent extraction showed doughnut-shaped unit particles with central depressions in negative staining and low angle rotary shadowing electron microscopy. (nii.ac.jp)
  • Cx43
  • In mammary glands, Cx26 and Cx32 in acinar cells and Cx43 in myoepithelial cells revealed dynamic changes of expression during pregnancy, parturition, lactation and weaning period at RNA transcription, protein production, and phosphorylation states, indicating the activity-related expression of gap junction proteins thus consituting functionally distinct cell groups. (nii.ac.jp)
  • Previous biochemical studies have been limited by the low levels of Cx43 protein in fibroblast cell lines. (hawaii.edu)
  • This phosphorylation event occurred in the COOH-tail region of the Cx43 molecule because pp60src phosphorylated a GST-fusion protein containing the COOH-tail region of Cx43 and the phosphotryptic peptides generated using this construct comigrated with the subset mentioned above. (hawaii.edu)
  • orthologous protein
  • p>An evidence describes the source of an annotation, e.g. an experiment that has been published in the scientific literature, an orthologous protein, a record from another database, etc. (uniprot.org)
  • Cx50
  • The extent to which gap junction proteins expressed in the lens, Cx46 and Cx50, accumulate PTMs with aging is not known. (arvojournals.org)
  • Enzyme-digested peptides were analyzed by liquid chromatography (LC)-electrospray tandem mass spectrometry (ESI/MS/MS). The functional effects of different PTMs on conductance, permeability and gating were assessed using electrophysiological measurements in cells expressing truncated Cx46 and Cx50 channel proteins. (arvojournals.org)
  • functional gap
  • In addition, we observed functional gap junction communication between platelets during platelet aggregation in vitro, as assessed by microinjection of the gap junction-permeable tracer neurobiotin in platelets isolated from human or wild-type mice. (ahajournals.org)
  • lens
  • A number of proteins in the lens undergo extensive post-translational modifications (PTMs) with age, leading to alterations in their function. (arvojournals.org)
  • In this study, we identified truncations in connexin proteins in the human lens using mass spectrometric analysis. (arvojournals.org)
  • Because the delivery of anti-oxidant molecules such as glutathione to the nucleus is dependent on gap junction channels, the decrease in coupling conductance due to PTMs might underlie the age-dependent decrease in glutathione levels in the lens core, ultimately leading to cataracts. (arvojournals.org)
  • channels
  • One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. (uniprot.org)
  • Vertebrate gap junction channels are thought to participate in diverse biological functions. (ebi.ac.uk)
  • Channel composition is thought to influence the function of gap junction channels. (wikipedia.org)
  • Typically the GJB1 protein forms channels through the myelin to the internal Schwann cell or oligodendrocyte, allowing effective transportation and communication. (wikipedia.org)