Protein expression rna. Medical search. Frequent questions

Our goal is to understand the molecular mechanisms whereby the genomic RNA of influenza-like viruses is, on the one hand, the template for transcription and replication of the viral genome by its RNA-dependent RNA polymerase and, on the other hand, an Achilles' heel, whose recognition as non-self can trigger an innate immune response to counter the viral infection. (embl.org)
87(5): 1663-1667] Specifically, the investigators started by priming whole cerebellar RNA with a synthetic oligonucleotide containing a T7 RNA polymerase promoter sequence. (genomicglossaries.com)
After second- strand cDNA synthesis, T7 RNA polymerase was used to generate aRNA. (genomicglossaries.com)
During evolution, this protein naturally lost its catalytic activity in Drosophila where Pcif1 is, like its human counterpart, expressed in the nucleus and associated with the C-terminal domain of RNA polymerase (RNA Pol II). (cea.fr)
This protein binds to the phosphorylated form of serine 5 of RNA polymerase II ( Figure ) and may directly modulate its activity or promote the recruitment of chromatin components. (cea.fr)
These results suggest a similar contribution of PCIF1 to the fine regulation of RNA polymerase II activity in mammals, in addition to its mRNA methylation activity, whose role in mRNA translation efficiency is actively studied but still controversial. (cea.fr)
T7 RNA polymerase is expressed from the lacUV5 promoter, which is less sensitive to catabolite repression than the wt lac promoter. (neb.com)

Previously, we worked on aminoacyl-tRNA synthetases, which play an essential role in protein synthesis by charging specifically their cognate tRNA(s) with the correct amino acid and editing mischarged amino acids if necessary (Palencia et al . (embl.org)
These studies are being extended to viral RNPs (the physiological RNA synthesis units) to understand the behaviour of the viral nucleoprotein during replication and transcription and to include host factors important for viral replication. (embl.org)
Sci Rep. 2018) and nucleoside analogue, RNA synthesis inhibitors (Kouba et al . (embl.org)
The results of these analyses revealed that the differentially expressed exosomal miRNAs participate in multiple biological processes, such as gene expression, synthesis of biomolecules, cell development, differentiation, and signal transduction, among others. (hindawi.com)
This expression is strictly dependent on the synthesis of messenger RNAs transcripts from the DNA molecule and their subsequent translation into proteins by the ribosomes. (cea.fr)
The text reviews the synthesis of DNA and genome engineering and offers a discussion of the parts and devices that control protein expression and activity. (wiley-vch.de)
The authors include information on the devices that support spatial engineering, RNA switches and explore the early applications of synthetic biology in protein synthesis, generation of pathway libraries, and immunotherapy. (wiley-vch.de)
The 84th Cold Spring Harbor Symposium focused on RNA Control & Regulation and provided a current synthesis of the enormous progress in our understanding of RNA's influence on cells and organisms and how, when aberrant, its effects may contribute to the progression of disease. (cshlpress.com)
Pretreatment with 5FU increased the production of protoporphyrin IX (the active photosensitizer) and proapoptotic p53 expression and upregulated the enzymes in the heme synthesis pathway, compared with control lesions. (aacr.org)
The complexes may induce transcription of messenger RNA, leading to the synthesis of new proteins. (bvsalud.org)
Protein synthesis, folding, and tertiary and quaternary structure ultimately determine much of the body's structure and function. (msdmanuals.com)

Most known RNA thermometers are located in the 5′ untranslated region (UTR) of messenger RNA encoding heat shock proteins-though it has been suggested this fact may be due, in part, to sampling bias and inherent difficulties of detecting short, unconserved RNA sequences in genomic data. (wikipedia.org)
It can be accessed online at www.helmholtz-hiri.de/en/datasets/enterococcus through an instance of the genomic viewer JBrowse. (frontiersin.org)
We investigate the functional relevance of de novo missense variants, specifically whether they are likely to disrupt protein interactions, and nominate novel genes in risk for ASD through integrated genomic, transcriptomic, and proteomic analyses. (springer.com)

Overall, our data suggest that butyrate can reduce the expression of inflammatory genes not only by transcriptional regulation, but also by post-transcriptional regulation via inhibition of mRNA stabilizing proteins. (metu.edu.tr)
Down-regulation of cold-inducible proteins including RBM3 in prostate cancer cells by exposure to hyperthermia enhanced cancer cell response to chemotherapy [ 13 ]. (biomedcentral.com)
While we and other labs have demonstrated the isolation of mRNA subsets that are potentially co-regulated using RNA binding proteins as reporters of gene expression, methods have not been described that provide information about coordinated posttranscriptional regulation within specific types of cells during tumorigenesis and development. (biomedcentral.com)
In agreement with these observations, the Pcif1 protein is distributed all along the polytene chromosomes at the level of active transcription sites and interferes with chromatin-dependent gene expression regulation. (cea.fr)
The lack of catalytic activity of Pcif1 in Drosophila reveals a new mechanism of gene expression regulation. (cea.fr)
Furthermore, it was confirmed that Beclin 1 expression by NS5A was involved in the negative regulation of starvation‑induced liver cancer apoptosis, which was accompanied by reduced p53 and apoptosis regulator Bax expression, as well as decreased caspase‑3/-7 activation. (spandidos-publications.com)
Regulation of gene expression by proteins and small RNAs. (otago.ac.nz)

Crucial to this process are a number of RNA-binding proteins, responsible for the posttranscriptional control of essential mRNAs and particularly pertinent to the two periods of inactive transcription that occur in spermatogenesis. (edu.au)
Prior to this research, mutations upstream from the transcription start site in a lambda (λ) phage cIII mRNA were found to affect the level of translation of the cIII protein. (wikipedia.org)
The candidate thermosensor heat shock RNA-1 (HSR1) activates heat-shock transcription factor 1 (HSF1) and induces protective proteins when cell temperature exceeds 37 °C (body temperature), thus preventing the cells from overheating. (wikipedia.org)
Transcription in eukaryotes is carried out by three main RNA polymerases: Pol I, II, and III. (mdpi.com)
Previous Cold Spring Harbor Symposia have addressed many different aspects of RNA biology such as Mechanisms of Transcription (1998), The Ribosome (2001), Epigenetics (2004) and Regulatory RNAs (2006). (cshlpress.com)
PRDM9 is a member of the PRDM family of transcription regulators, but unlike other family members, it contains a Krüppel-associated box (KRAB)-related domain that is predicted to be a potential protein interaction domain. (springer.com)
Transcription factors bind alongside the promoter and set off the gene expression. (lu.se)
Transcription factors are a group of proteins that are needed for a gene to start being copied to RNA, i.e. for transcription to start. (lu.se)

MicroRNAs (miRNAs) are small non-coding nucleic acids that can regulate post-transcriptional gene expression by binding to complementary sequences of target mRNA. (birmingham.ac.uk)
In this study, we combined the results of two independent systematic reviews aiming to unveil the co-expression network of miRNAs and proteins in brain tissues of AD patients. (birmingham.ac.uk)
Twenty-eight studies including a total of 113 differentially expressed miRNAs (53 of them validated by qRT-PCR), and 26 studies including a total of 196 proteins differentially expressed in AD brains compared to healthy age matched controls were selected. (birmingham.ac.uk)
Epstein-Barr Virus (EBV) infection induces cellular non-coding (nc)RNAs e.g., the "vault" RNAs or miRNAs such as miR-21, miR-155 or miR-146a. (nih.gov)
This study confirms that alterations in the expression of exosomal miRNAs can promote osteogenic differentiation of ADSCs, which also provides the foundation for further research on the regulatory functions of exosomal miRNAs in the context of ADSC osteogenesis. (hindawi.com)
The miRNAs are small stretches of RNA made by host cells to regulate gene expression. (feedstuffs.com)
To see if RNA viruses other than hepatitis C use host miRNA, the researchers developed a screening method that chemically attaches those miRNAs to their targets. (feedstuffs.com)
Six miRNAs had concordant expression in both tumors and breast cancer patient blood samples compared with the normal control samples. (cdc.gov)
Twelve miRNAs showed concordant expression in tumors vs. normal breast tissues and patient survival (n = 1093), with seven as potential tumor suppressors and five as potential oncomiRs. (cdc.gov)
From experimentally validated target genes of these 86 miRNAs, pan-sensitive and pan-resistant genes with concordant mRNA and protein expression associated with in-vitro drug response to 19 NCCN-recommended breast cancer drugs were selected. (cdc.gov)
MicroRNAs (miRNAs) are an integral part of the post-transcriptional machinery of gene expression and have been implicated in the carcinogenic cascade. (cdc.gov)
MicroRNAs (miRNAs) are small, noncoding RNAs that bind to the 3' UTR of target mRNAs, and silence gene expression by inducing degradation of target mRNAs or inhibition of protein translation [ 10 ]. (cdc.gov)

In this study, we screened the whole genome of Aedes aegypti , the primary vector of several pathogenic viruses , and identified the proteins containing RNA recognition motif (RRM), the most abundant protein domain in eukaryotes . (bvsalud.org)
Our study provides high-resolution structures of medically relevant FluPol A , as well as insights into the replication mechanisms of the viral RNA genome. (nature.com)
In living organisms, gene expression is a complex process that results in the production of proteins from the genome in a time- and space-regulated manner. (cea.fr)
A genome-wide in situ hybridization map of RNA-binding proteins reveals anatomically restricted expression in the developing mouse brain. (nih.gov)

RNA-binding proteins (RBPs) are the proteins that bind RNAs and regulate their functioning. (bvsalud.org)
aegypti RRM-containing RBPs included serine / arginine -rich (SR) proteins , polyadenylate- binding proteins (PABP), heteronuclear ribonucleoproteins ( hnRNP ), small nuclear ribonucleoproteins ( snRNP ), splicing factors , eukaryotic initiation factors , transformers, and nucleolysins. (bvsalud.org)
Expression analysis of the genes encoding RBPs using publicly available RNA sequencing data for different developmental time points of the mosquito life cycle starting from the ovary and eggs up to the adults revealed stage-specific expression with several genes preferentially expressed in early embryonic stages and blood -fed female ovaries . (bvsalud.org)
We have hypothesized that butyrate may regulate gene expression post-transcriptionally through global effects on the expression or cytoplasmic trans location of RBPs. (metu.edu.tr)
Using bioinformatics analyses of publicly available microarray data as well as colon cancer cell lines treated with sodium butyrate, we have observed that butyrate treatment led to a general reduction in expression of several (but not all) RBPs and inhibition in the cytosolic translocation of HuR, a well-known stabilizing RBP. (metu.edu.tr)
Many RNA binding proteins (RBPs) including the YTH family of proteins modulate the effects of m6A through specific binding to the methylated RNA. (diferencias-entre.org)

The cell counters RNA viruses with innate immune pattern-recognition receptors, such as the RNA helicase RIG-I, which recognise particular viral RNA structural motifs (e.g. 5′ triphosphate-dsRNA) as non-self, thus activating a signalling pathway leading to interferon production and establishment of the anti-viral state. (embl.org)
In response, viruses deploy proteins as counter-counter-measures to dampen the immune response, for instance, by supressing the RIG-I signalling pathway. (embl.org)
While no inverse relationships between miR-132-3p and proteins were found, miR-16′s inverse relationship with CLOCK proteins in the circadian rhythm pathway is discussed and therapeutic targets are proposed. (birmingham.ac.uk)
One pathway, which has been extensively studied in yeast, is mainly guided by chromatin structure and the other, analyzed in detail in mice, is driven by the sequence-specific DNA-binding PR domain-containing protein 9 (PRDM9). (springer.com)
However, like most CLN proteins, the association of CLN5 with a defined biological pathway is still under investigation. (frontiersin.org)

In parallel, we are doing the same for viral replication, which is unprimed and occurs in two-steps via an intermediate complementary RNA (cRNA). (embl.org)
Within some glioblastomas probed with PDGFR-α complementary RNA, cells heavily loaded with grains were intermingled with others containing low or moderate signals. (aacrjournals.org)
RNA molecules which hybridize to complementary sequences in either RNA or DNA altering the function of the latter. (genomicglossaries.com)
RNA-like oligonucleotides that are complementary to a portion of a target mRNA molecule. (genomicglossaries.com)
More specifically, antisense oligonucleotides that are useful as reagents for target validation , or as drugs, are engineered molecules that differ from natural RNA but that have a base sequence that is recognized as being complementary to a very specific mRNA sequence. (genomicglossaries.com)
A complementary RNA sequence that binds to a naturally occurring (sense) mRNA molecule, thus blocking its translation. (genomicglossaries.com)
Highly specific and effective gene silencing of any disease can be achieved by an accurate knowledge of the target mRNA sequence and rational design of its complementary antisense agents for the downregulation of its protein message. (genomicglossaries.com)
Genes encoding disrupted complementary interactors tend to be risk genes, and an interaction network built from these proteins is enriched for ASD proteins. (springer.com)

Overall this study provides further evidence for the unique functions that RNA-binding protein isoforms occupy within spermatogenesis, and introduces the potential manipulation of the Musashi family proteins to elucidate the mechanisms of posttranscriptional gene expression during germ cell development. (edu.au)
Endogenous antisense RNAs function as regulators of gene expression by a variety of mechanisms. (genomicglossaries.com)
In order to understand how different cell types influence one another's gene expression, it will be necessary to monitor the mRNA profiles of each cell type independently and to dissect the mechanisms that regulate their gene expression outcomes. (biomedcentral.com)
This study demonstrates the importance to not restrict the function of enzymes to their sole catalytic role in the living world and the interest of thoroughly examining the evolution of proteins in eukaryotes in order to discover unsuspected or hidden mechanisms of action. (cea.fr)
MICR 335 will provide a fundamental knowledge of the mechanisms that bacteria use to sense their environment and adapt their gene expression to optimise their growth and survival. (otago.ac.nz)
Overall, this study reveals the impact of cln5 -deficiency on gene expression in D. discoideum , provides insight on the genes and proteins that play a role in regulating Cln5-dependent processes, and sheds light on the molecular mechanisms underlying CLN5 disease. (frontiersin.org)
As well as DNA methylation, there are also other epigenetic mechanisms such as modification of histone proteins. (lu.se)
These elements appear to influence the expression of both protein-coding genes and non-coding transcripts in the human brain through various mechanisms," says Raquel Garza. (lu.se)

When the interaction propensity score of a disordered region exceeds that of the full-length protein (points above the red line), disorder is considered to promote interaction with RNA molecules. (biomedcentral.com)
Because alternative RNA splicing allows different types of mRNA molecules to be created from a single gene, it generates the diversity of protein function and structure that is essential to complex organisms. (genomicglossaries.com)
This protein is one part (subunit) of the ribonucleoprotein complex, which is composed of proteins and molecules of RNA, DNA's chemical cousin. (medlineplus.gov)
By utilizing in vitro transcribed (IVT) messenger RNA (mRNA) molecules, scientists can deliver specific instructions to cells, directing them to produce therapeutic proteins or trigger desired immune responses. (selectscience.net)
Among these proteins, annexin is known to inhibit phospholipase A2 and thus block the production of mediators and arachidonic acid metabolites such as the COX-2 enzyme, leukotrienes and prostaglandins, cytokines, interleukins, adhesion molecules and enzymes such as collagenase 24,25 . (bvsalud.org)
Gene expression generally produces proteins, but there are also non-coding genes that produce RNA molecules (rRNA, tRNA). (lu.se)
It is not only the genes that code for proteins that are methylated, but also parts of the DNA that code for regulatory functions (RNA molecules), which can have a greater effect than genes. (lu.se)

In this study we recognized/verified METTL16 focuses on by immunoprecipitation of both endogenous as well as exogenous FLAG-tagged protein. (diferencias-entre.org)
Interestingly, exogenously overexpressed METTL16 differed from your endogenous protein in its relative affinity for RNA focuses on which prompted us to investigate METTL16's localization within the cell. (diferencias-entre.org)
Native membrane derived polymer-supported lipid bilayers (nSLBs) are poised to bridge the gap between live cell experiments and traditional model membrane architectures that by offering a combination of accessibility by surface sensitive analytical instrumentation and a composition which more closely resembles cellular membranes by displaying a diversity of endogenous membrane proteins, lipids, and carbohydrates. (lu.se)
Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)

An RNA thermometer (or RNA thermosensor) is a temperature-sensitive non-coding RNA molecule which regulates gene expression. (wikipedia.org)
Using the Caco-2 cell line, which undergoes spontaneous re-differentiation when grown past confluency, we observed a loss of VCAM-1 (vascular cell adhesion molecule 1) mRNA expression, while ICAM-1 (intercellular cell adhesion m. (metu.edu.tr)
This molecule acts as a genetic blueprint for protein production. (medlineplus.gov)
A gene, the basic unit of heredity, is a segment of DNA containing all the information necessary to synthesize a polypeptide (protein) or a functional RNA molecule. (msdmanuals.com)
A gene is a segment of DNA that provides the code to construct a protein or RNA molecule. (msdmanuals.com)

The Cusack group uses X-ray crystallography and cryo electron-microscopy (cryoEM) to study the structural biology of protein-RNA complexes involved in RNA virus replication, innate immunity and cellular RNA metabolism. (embl.org)
USP15 Deubiquitinates TUT1 Associated with RNA Metabolism and Maintains Cerebellar Homeostasis. (nih.gov)

Untranslated regions (UTRs) of protein-coding transcripts are colored gray. (proteinatlas.org)
RNA binding proteins can control protein expression by regulating transport, localization, splicing, polyadenylation and turnover of mRNA transcripts. (umassmed.edu)

Utilizing our previous interactome perturbation predictor, we identify a set of missense variants that are likely disruptive to protein-protein interactions. (springer.com)
For genes encoding the disrupted interactions, we evaluate their expression patterns across developing brains and within specific cell types, using both bulk and inferred cell-type-specific brain transcriptomes. (springer.com)
Finally, we integrate protein interactions and cell-type-specific co-expression networks together with published association data to implicate novel genes in ASD risk in a cell-type-specific manner. (springer.com)
Extending earlier work, we show that de novo missense variants that disrupt protein interactions are enriched in individuals with ASD, often affecting hub proteins and disrupting hub interactions. (springer.com)
Consistent with other studies, genes identified by disrupted protein interactions are expressed early in development and in excitatory and inhibitory neuronal lineages. (springer.com)
Disrupted protein interactions identify gene sets involved in risk for ASD. (springer.com)
To find out, he and his colleagues used CLEAR-CLIP to analyze the interactions of 15 RNA viruses with their host miRNA. (feedstuffs.com)

RNA thermometers, along with riboswitches, are used as examples in support of the RNA world hypothesis. (wikipedia.org)

Synthetic RNA thermometers have been designed with just a simple single-hairpin structure. (wikipedia.org)

(B) For 66% of the proteins (137 entries), disorder contributes at low interaction propensities, while full-length protein sequences dominate at high interaction propensities (Mann-Whitney U test). (biomedcentral.com)
In the nucleus, these complexes bind to specific DNA sequences and alter their expression. (bvsalud.org)
This approach helped us to address these highly repetitive sequences, usually masked in standard bioinformatics pipelines, allowing us to accurately measure LINE-1 expression in each cell type found in our samples," explains Raquel Garza, co-first author of the study. (lu.se)

Niraparib hydrochloride It can also bind and methylate the long noncoding RNAs MALAT1 and XIST [28, 30]. (diferencias-entre.org)
The mutated GlmS protein does not bind Ni-NTA resin in the presence of 20mM imidazole binding/ wash buffer, whereas wt GlmS protein binds Ni-NTA resin and is not eluted until the imidazole concentration is within 55-80 mM (1). (neb.com)

These mitochondrial responses are governed by general principles of regulatory biology exemplified by changes in gene expression, protein translation, protein complex formation, transmembrane transport, enzymatic activities and metabolite levels. (nature.com)
Izhaki-Tavor, LS & Dessau, M 2020, ' ATP-dependent RNA helicase domain of the ZC3H41 protein from Trypanosoma brucei: Expression, purification and crystallization ', Acta Crystallographica Section F:Structural Biology Communications , vol. 76, pp. 604-608. (iucc.ac.il)
This comprehensive global TSS mapping atlas provides a valuable resource for RNA biology and gene expression analysis in the Enterococci. (frontiersin.org)
Now a large and ever increasing number of genetic subtypes has been described, and major advances in molecular and cellular biology have clarified the understanding of the role of different proteins in the physiology of peripheral nerve conduction in health and in disease. (medscape.com)

Detailed structural analysis of the ROSE RNA thermometer revealed that the mismatched bases are actually engaged in nonstandard basepairing that preserves the helical structure of the RNA (see figure). (wikipedia.org)

The MIR1292 gene provides instructions for making a type of RNA that regulates the activity (expression) of genes that produce proteins called glutamate receptors. (medlineplus.gov)

A biological process that is crucial for gene expression in most animal life, including humans. (genomicglossaries.com)
However, METTL16 (METT10D) has also been identified as an RNA m6A methyltransferase that can methylate both coding and noncoding RNAs, but its biological role remains unclear. (diferencias-entre.org)
When used together, these research tools enable researchers to simultaneously analyze RNA and proteins in thousands of individual cells and develop a more complete picture of the role genes and proteins play in biological systems. (bd.com)
Characterization of the structure and dynamics of protein-RNA complexes is essential for understanding how they function at the molecular level: how these proteins specifically recognize their target RNAs, what are the structure-function relationships, and assess their biological role in regulating gene expression. (umassmed.edu)
Characterization of the structure and dynamics of protein-RNA complexes is essential for understanding how they function at the molecular level: how these proteins specifically recognize their target RNAs, what are the structure-function relationships, and to assess their biological role in regulating gene expression. (umassmed.edu)
To improve our understanding of the biological and clinical significance of somatic mutations on RNA processing in cancer, the authors developed a new method, known as MIRA (mutation identification for RNA alterations). (aacr.org)

RNA thermometers often regulate genes required during either a heat shock or cold shock response, but have been implicated in other regulatory roles such as in pathogenicity and starvation. (wikipedia.org)

The expression of platelet-derived growth factor (PDGF) and its receptors was analyzed in 14 gliomas of various degrees of malignancy and compared with three gliosis cases by in situ hybridization and immunohistochemistry techniques. (aacrjournals.org)

Bacterial RNA maturation. (otago.ac.nz)

Liu et al ( 13 ) reported that Beclin 1 gene deletion by either RNA interference or the autophagy inhibitor 3-methyladenine (MA) significantly enhances melatonin-induced apoptosis in mouse hepatoma H22 cells. (spandidos-publications.com)
We further demonstrated that suppression of autophagy using either pharmacological inhibitors or RNA interference of essential autophagy genes enhanced cell death induced by IM in cell lines and primary CML cells. (lu.se)

Microscopy analysis of tumour samples is commonly performed on fixed, thinly sectioned and protein-labelled tissues. (elsevierpure.com)
We used DIIFCO to spatially profile the expression of diverse coding RNAs and non-coding RNAs at the single-cell resolution in a variety of cancer tissues. (elsevierpure.com)
The present study analyzes the putative prognostic role of RBM3 in a large cohort of patients with urothelial carcinoma (UCA) of the bladder by analyzing RBM3 immunohistochemical expression in various benign urothelial tissues, as well as in invasive and metastatic urothelial lesions. (biomedcentral.com)
Tumors and complex tissues consist of mixtures of communicating cells that differ significantly in their gene expression status. (biomedcentral.com)
RNA-binding proteins can be used as reporters to elucidate components of operational mRNA networks and operons involved in regulating cell-type specific gene expression in tissues and tumors. (biomedcentral.com)
They interact with specific intracellular receptor proteins in the target tissues and alter the expression of genes to corticosteroids. (bvsalud.org)
The gene expression varies between different types of cell and when these epigenetic changes are inherited by daughter cells through cell division in the womb, different tissues and organs are built. (lu.se)

We have extended our work on viral polymerases to those of other segmented negative-strand RNA viruses such as the large order of Bunyavirales . (embl.org)
Now, the same team - including researchers in the laboratories of Charles M. Rice, the Maurice R. & Corinne P. Greenberg professor and head of the Laboratory of Virology & Infectious Disease, and Robert B. Darnell, the Robert & Harriet Heilbrunn professor and head of the Laboratory of Molecular Neuro-oncology - has found evidence that another RNA virus - bovine viral diarrhea (BVD) virus, which infects cows and other livestock - also depends on miRNA for infection. (feedstuffs.com)

A fragment of the Trypanosoma brucei ZC3H41 protein encompassing the ATP-dependent RNA helicase domain was successfully subcloned for expression in a bacterial system (Escherichia coli). (iucc.ac.il)

Beclin l, the mammalian counterpart of the yeast Atg6 gene, is an essential protein in autophagy ( 10 ). (spandidos-publications.com)

Despite their critical implication in worldwide public healthcare, essential and available resources such as deep transcriptome annotations remain poor, which also limits our understanding of post-transcriptional control small regulatory RNA (sRNA) functions in these bacteria. (frontiersin.org)

DSB sites are preferentially located within chromatin loops, while several proteins that are required for DSB formation (Rec114, Mei4, and Mer2) localize on the chromosome axis (Blat et al. (springer.com)
The protein structure that surrounds the DNA, chromatin, largely comprises histone proteins and can be open or closed and thus allow or prevent factors binding to the DNA. (lu.se)

Therefore, while METTL16 has been reported to be a nuclear protein, our findings suggest that METTL16 is also a cytoplasmic methyltransferase that may alter its RNA binding Niraparib hydrochloride preferences depending on its cellular localization. (diferencias-entre.org)
Future studies will seek to confirm variations between cytoplasmic and nuclear RNA Niraparib hydrochloride focuses on in addition to exploring the physiological part of METTL16 through long-term knockdown. (diferencias-entre.org)
Orthologous to human LSM7 (LSM7 homolog, U6 small nuclear RNA and mRNA degradation associated). (nih.gov)
In addition, NS5A significantly increases the expression of inducible nitric oxide synthase, cyclin D1 and nuclear factor-κB, but decreases p53 protein expression in HepG2 cells ( 6 ). (spandidos-publications.com)

The fly Pcif1 expressed in the nucleus and binds the phosphorylated C-terminal domain (CTD) of RNA Pol II (at the level of phosph orylated s erine 5). (cea.fr)
When a microRNA is present in the cell, it binds to the target sites and downregulates GFP expression, while in cells that do not express the microRNA GFP, it is expressed. (lu.se)

RNA which contains an intron sequence that has an enzyme- like catalytic activity. (genomicglossaries.com)

Methyltransferase like -3 and -14 (METTL3 and METTL14) and Wilms tumor associating protein (WTAP) in addition to KIAA1429 are all components of the mRNA m6A methyltransferase complex, which uses a S-adenosyl methionine (SAM) binding website on METTL3 to methylate specific mRNAs for methylation having a RRACH m6A consensus sequence [10C15]. (diferencias-entre.org)
Here we demonstrate that RNA-binding proteins can be used to isolate mRNA populations representing total cell mRNA from specific types of cells, as well as discrete mRNA subpopulations that represent post-transcriptionally regulated subsets of mRNAs that encode functionally related proteins. (biomedcentral.com)
For example, we identified subpopulations of mRNAs that are associated with ELAV/Hu RNA-binding proteins that are expressed in specific cell types [ 10 ]. (biomedcentral.com)
Moreover, because many different mRNA-binding proteins in specific cell types are known to interact with unique subpopulations of mRNAs encoding functionally related proteins [ 9 - 15 ] they can be informative of the dynamic effects of cells on one another. (biomedcentral.com)
In this study, we define a model system for using poly (A) binding protein (PABP) to recover mRNAs from specific cell-types in mixed cell cultures. (biomedcentral.com)
Analyzing bacteria in late exponential phase, we capture ~40% ( E. faecalis ) and 43% ( E. faecium ) of the annotated protein-coding genes, determine 5′ and 3′ UTR (untranslated region) length, and detect instances of leaderless mRNAs. (frontiersin.org)
As they are synthesized, mRNAs undergo chemical modifications that ensure their stability, their delivery to ribosomes and eventually their translation into proteins. (cea.fr)

Cells deficient in both these proteases are much more amenable to the production of proteins from cloned genes. (neb.com)

Mice lacking mitochondrial uncoupling protein are cold-sensitive but not obese. (nature.com)

FRANKLIN LAKES, N.J., July 26, 2018 /PRNewswire/ -- BD (Becton, Dickinson and Company) (NYSE: BDX), a leading global medical technology company, today announced the commercial release of the BD ® AbSeq assay to analyze protein expression at the single-cell level using high-throughput sequencing. (bd.com)

One way wily viruses can get a leg up is by subverting the micro-RNA (miRNA) of their host. (feedstuffs.com)

Hepatitis C virus (HCV) nonstructural protein 5A (NS5A) modulates cellular apoptosis, which is involved in the occurrence and development of liver cancer. (spandidos-publications.com)
By regulating the expression of several genes in host liver cells, NS5A also induces cellular proliferation, and influences the curative effect of interferon ( 7 ). (spandidos-publications.com)

Researchers at IRIG, in collaboration with the University of Geneva, focused on a RNA mammalian methyltransferase, the PCIF1 protein (homologous to the Drosophila Pcif1 protein), which adds an extra methyl group to m 6 A (m 6 adenosine) to form m 6 Am when the first transcribed nucleotide is an adenosine. (cea.fr)

It seems that not until the technologies for working with nucleic acids and proteins are better integrated will their researchers be more connected than they are now. (genomicglossaries.com)
Researchers at Stanford University used such a method to produce amplified heterogeneous populations of RNA from limited quantities of cDNA. (genomicglossaries.com)
More recently, another group of researchers reported that they had developed a process for optimizing low- abundance RNA, by combining aRNA amplification with template- switching. (genomicglossaries.com)
The assay brings together high-quality antibodies from the BD Pharmingen™ portfolio with oligonucleotides, allowing researchers to perform single-cell protein analysis on the BD Rhapsody™ single-cell analysis system . (bd.com)
In conjunction with RNA sequencing data, the researchers used MIRA to identify mutations in binding sites that altered RNA expression and splicing. (aacr.org)

Short chain fatty acids (SCFAs) are produced by commensal bacteria in the gut and are known to reduce inflammation through transcriptional inhibition of cytokines and inflammatory proteins such as cyclooxygenase-2 (COX-2). (metu.edu.tr)
As seen with many other bacteria, RNA-seq technology has now become the method of choice for transcriptome analysis in the enterococcus. (frontiersin.org)

By transporting "cargos" such as proteins, RNAs, DNAs, and lipids [ 26 ], exosomes regulate the eventual fate of recipient cells. (hindawi.com)
Due to the lack of detergent solubilization and reconstitution steps, the nSLBs created using this approach contain essentially all of the native lipids, as well as the membrane-associated proteins and carbohydrates from the donor membrane. (lu.se)

Coding RNA is used to make proteins. (cdc.gov)
Non-coding RNA helps control gene expression by attaching to coding RNA, along with certain proteins, to break down the coding RNA so that it cannot be used to make proteins. (cdc.gov)
Epigenetics allows the muscle cell to turn "on" genes to make proteins important for its job and turn "off" genes important for a nerve cell's job. (cdc.gov)

METTL16 has also been identified as an RNA m6A methyltransferase that methylates both coding and noncoding RNAs. (diferencias-entre.org)
Studies carried out by scientists at IRIG, in collaboration with the University of Geneva, on the fruit fly model Drosophila melanogaster have revealed the role of Pcif1 in the control of gene expression, despite the fact that this protein has completely lost its RNA methyltransferase activity compared to its mammalian counterpart PCIF1. (cea.fr)

We show that the EBV-encoded latent membrane protein 1 (LMP1) induces the expression of miR-146a via NFkappaB. (nih.gov)
While many studies have been focused on the membrane structure, the dynamics of such systems are crucial for the function of the membrane including membrane bound proteins. (lu.se)

Examples of RNA thermometers include FourU, the Hsp90 cis-regulatory element, the ROSE element, the Lig RNA thermometer, and the Hsp17 thermometer. (wikipedia.org)
NEAT1) sequester various regulatory proteins regulating gene expression including RNA modification (e.g. (bvsalud.org)

RNA-binding ability and structural disorder. (biomedcentral.com)
This structural transition can then expose or occlude important regions of RNA such as a ribosome binding site, which then affects the translation rate of a nearby protein-coding gene. (wikipedia.org)
The collected data revealed preliminary structural information regarding this newly discovered protein. (iucc.ac.il)

Today we use epigenetics to mean changes to gene expression that are not caused by changes in the actual DNA sequence. (lu.se)

In silico identification, characterization, and expression analysis of RNA recognition motif (RRM) containing RNA-binding proteins in Aedes aegypti. (bvsalud.org)
RNA-binding motif protein 3 (RBM3), involved in cell survival, has paradoxically been linked to both oncogenesis as well as an increased survival in several cancers, including urothelial carcinoma (UCA). (biomedcentral.com)

Strains for which quantitative PCR results and second immunoglobulin-binding protein expression was analyzed by Western blot. (cdc.gov)

One basic problem is how a decrease in tRNA levels, by downregulating Pol III efficiency, influences the expression pattern of protein-coding genes. (mdpi.com)

These numerous modifications provide an additional level of information for the control of protein production and collectively form the epi-transcriptome. (cea.fr)

aRNA is RNA that is transcribed from the coding, rather than the template, strand of DNA. (genomicglossaries.com)
The expressed fusion protein is 82 kDa whereas the wt ArnA protein is 74 kDa. (neb.com)

While genetic changes can alter which protein is made, epigenetic changes affect gene expression to turn genes "on" and "off. (cdc.gov)
Epigenetic changes affect gene expression in different ways. (cdc.gov)
Epigenetic changes mainly take place through chemical modification of DNA (DNA methylation) and histones, the proteins around which the DNA helix winds itself. (lu.se)

DNA wraps around proteins called histones. (cdc.gov)
When histones are tightly packed together, proteins that 'read' the gene cannot access the DNA as easily, so the gene is turned "off. (cdc.gov)
When histones are loosely packed, more DNA is exposed or not wrapped around a histone and can be accessed by proteins that 'read' the gene, so the gene is turned "on. (cdc.gov)
Non-coding RNA may also recruit proteins to modify histones to turn genes "on" or "off. (cdc.gov)
Epigenetic modifications of the histone proteins, such as acetylation or methylation, affect how tightly the DNA is packed around the histones and thus how accessible the DNA sequence is. (lu.se)

Here, removal of intact α 6 protein from cerebellar granule cells allowed perturbations in other subunit levels to be studied. (jneurosci.org)

PR domain-containing protein 9 (PRDM9) is a major regulator of the localization of meiotic recombination hotspots in the human and mouse genomes. (springer.com)

This study highlights an important contribution for Pcif1 in gene expression and physiology of the organism independently of any catalytic activity. (cea.fr)
The work of the scientists shows that mutation of Pcif1 gene in Drosophila results in a deregulation of the expression of a set of genes, reduced body weight and a significant drop in fertility which indicates an important role for this protein in the physiology of the organism. (cea.fr)

Anatomy18

Organisms14

Diseases10

Chemicals and Drugs107

Analytical, Diagnostic and Therapeutic Techniques and Equipment34

Phenomena and Processes72

Disciplines and Occupations2

Information Science3

Polymerase7

Synthesis11

Genomic3

Regulation7

Transcription8

MiRNAs12

Genome4

RBPs6

Pathway5

Complementary8

Mechanisms8

Molecules7

Endogenous4

Molecule5

Metabolism2

Transcripts2

Interactions7

Riboswitches1

Synthetic RNA1

Sequences3

Bind2

Biology4

Helical structure1

Regulates1

Biological6

Pathogenicity1

Situ hybridization1

Maturation1

Interference2

Tissues7

Viral2

Helicase1

Autophagy1

Post-transcriptional1

Chromatin2

Nuclear4

Binds2

Intron1

MRNAs7

Production of proteins1

Mitochondrial1

Analyze1

Viruses1

Cellular2

Adenosine1

Researchers5

Bacteria2

Lipids2

Make proteins3

Methyltransferase2

Membrane2

Regulatory2

Structural3

Epigenetics1

Motif2

Immunoglobulin-binding1

TRNA1

Transcriptome1

ARNA2

Epigenetic changes3

Histones5

Cerebellar1

Localization1

Physiology2