• enzyme
  • 684. Katunuma N., Kito K-, Komtnami E., A new enzyme that specifically inactivates apo-protein of NAD-dependent dehydrogenases. (ximicat.com)
  • Jr., Application of a theory of enzyme specif it to protein synthesis. (ximicat.com)
  • However, medium supplementation with hemin proved to be an efficient strategy to increase the yield of active enzyme, whereas supplementation with the commonly used precursor 5-aminolevulinic acid did not affect target protein yield. (biomedcentral.com)
  • Thereby, secreted apo-species of the target protein are effectively saturated with cofactor, maximizing the yield of target enzyme activity. (biomedcentral.com)
  • Lipoate-protein ligase (EC 2.7.7.63, LplA, lipoate protein ligase, lipoate-protein ligase A, LPL, LPL-B) is an enzyme with systematic name ATP:lipoate adenylyltransferase. (wikipedia.org)
  • This enzyme catalyses the following chemical reaction (1) ATP + lipoate ⇌ {\displaystyle \rightleftharpoons } diphosphate + lipoyl-AMP (2) lipoyl-AMP + apoprotein ⇌ {\displaystyle \rightleftharpoons } protein N6-(lipoyl)lysine + AMP This enzyme requires Mg2+ as a cofactor. (wikipedia.org)
  • binds
  • This protein domain is of huge significance since it binds to fibrinogen, a glycoprotein involved in important processes such as haemostasis and coagulation. (wikipedia.org)
  • When a transferrin protein loaded with iron encounters a transferrin receptor on the surface of a cell, e.g., erythroid precursors in the bone marrow, it binds to it and is transported into the cell in a vesicle by receptor-mediated endocytosis. (wikipedia.org)
  • enzymatic
  • All cells use and rely on fats and cholesterol as building-blocks to create the multiple membranes that cells use both to control internal water content and internal water-soluble elements and to organize their internal structure and protein enzymatic systems. (wikipedia.org)
  • Structures
  • The PDBFlex database explores the intrinsic flexibility of protein structures by analyzing structural variations of the same protein across the archive. (rcsb.org)
  • DNA can sometimes occur as single strands (often needing to be stabilized by single-strand binding proteins) or as A-form or Z-form helices, and occasionally in more complex 3D structures such as the crossover at Holliday junctions during DNA replication. (wikipedia.org)
  • RNA, in contrast, forms large and complex 3D tertiary structures reminiscent of proteins, as well as the loose single strands with locally folded regions that constitute messenger RNA molecules. (wikipedia.org)
  • soluble
  • Therefore, the high-precision electrophoresis and other native separation techniques are highly relevant as initial step of protein analysis and trace metal speciation followed by mass spectrometric and magnetic resonance methods for quantifying and identifying the soluble proteins of interest. (wikipedia.org)
  • degradation
  • Notably, LOV-based optogenetic tools have been gaining wide popularity in recent years to control a myriad of cellular events, including cell motility, subcellular organelle distribution, formation of membrane contact sites, and protein degradation. (wikipedia.org)
  • To elaborate, LRP1 mainly contributes to regulation of protein activity by binding target proteins as a co-receptor, in conjunction with integral membrane proteins or adaptor proteins like uPA, to the lysosome for degradation. (wikipedia.org)
  • nucleic
  • A biomolecular target (most commonly a protein or nucleic acid) is a key molecule involved in a particular metabolic or signaling pathway that is associated with a specific disease condition or pathology or to the infectivity or survival of a microbial pathogen. (wikipedia.org)
  • bound
  • Most strikingly, a CueR molecule coming from solution can directly substitute for a DNA-bound CueR or assist the dissociation of the incumbent CueR, both of which are unique examples for any DNA-binding protein. (pnas.org)
  • The kinetics of the direct protein substitution and assisted dissociation reactions indicate that these two unique processes can provide efficient pathways to replace a DNA-bound holo-CueR with apo-CueR, thus turning off transcription promptly and facilely. (pnas.org)
  • When not bound to iron, transferrin is known as "apotransferrin" (see also apoprotein). (wikipedia.org)
  • Biochemistry
  • QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a high-resolution and a highly accurate technique applied in biochemistry and bioinorganic chemistry to separate proteins quantitatively by isoelectric point. (wikipedia.org)
  • annotation
  • p>An evidence describes the source of an annotation, e.g. an experiment that has been published in the scientific literature, an orthologous protein, a record from another database, etc. (uniprot.org)
  • molecules
  • It is likely that the heterogeneity of the P450 pool arises from protein-protein interactions between different P450 molecules and between P450 and its redox partners. (aspetjournals.org)
  • In addition to small molecules, biopharmaceuticals and especially therapeutic antibodies are an increasingly important class of drugs and computational methods for improving the affinity, selectivity, and stability of these protein-based therapeutics have also been developed. (wikipedia.org)
  • Proteins perform several functions in living organisms, including catalytic reactions and transport of molecules or ions within the cells, the organs or the whole body. (wikipedia.org)
  • encodes
  • The LRP1 gene encodes a 600 kDa precursor protein that is processed by furin in the trans-Golgi complex, resulting in a 515 kDa alpha-chain and an 85 kDa beta-chain associated noncovalently. (wikipedia.org)
  • complex
  • The methylotrophic yeast Pichia pastoris is a valuable host for the recombinant production of complex proteins. (biomedcentral.com)
  • While the photochemical reactivity of the LOV2 domain has been found to be essential for the activation of the kinase, the in vivo functionality of the LOV1 domain within the protein complex still remains unclear. (wikipedia.org)
  • SdrG as an apoprotein and in complex ligand binding activity peptide analogous to its binding site in Fg. (wikipedia.org)
  • mitochondrial
  • The HCCS protein is localized to the inner mitochondrial membrane and is expressed in multiple tissue including prominently in the cardiovascular system and the central nervous system. (wikipedia.org)
  • membrane
  • This membrane probably undergoes some modification to its protein content, which functions to limit or prevent phago-lysosome fusion. (wikipedia.org)
  • commonly
  • The drug is most commonly an organic small molecule that activates or inhibits the function of a biomolecule such as a protein, which in turn results in a therapeutic benefit to the patient. (wikipedia.org)
  • stability
  • A high reproducibility in gel pore size and a high-yield electroelution of proteins performed by this highly reliable technique strongly correlate with the polymerization time of the acrylamide gels representing an inherent stability constant of the separation system. (wikipedia.org)
  • contains
  • The whole SdrG protein contains two domains, a N-terminal one named N2 domain and the C-terminal one named N3. (wikipedia.org)
  • Due to the specific properties of the prepared gel and electrophoresis buffer solution (which is basic and contains Tris-HCl and NaN3), most proteins of a biological system (e.g. (wikipedia.org)
  • Molecular
  • In molecular biology, the protein domain SdrG C terminal refers to the C terminus domain of an adhesin found only on the cell walls of bacteria. (wikipedia.org)
  • different
  • p>When browsing through different UniProt proteins, you can use the 'basket' to save them, so that you can back to find or analyse them later. (uniprot.org)
  • The aim of isoelectric focusing (IEF), for example, is to separate proteins according to their isoelectric point (pI), thus, according to their charge at different pH values. (wikipedia.org)
  • Although the pH value (10.00) of the electrophoresis buffer does not correspond to a physiological pH value within a cell or tissue type, the separated ring-shaped protein bands are eluted continuously into a physiological buffer solution (pH 8.00) and isolated in different fractions (see figure Electropherogram). (wikipedia.org)
  • domain
  • PDP: protein domain parser. (rcsb.org)
  • A Light-oxygen-voltage-sensing domain (LOV domain) is a protein sensor used by a large variety of higher plants, microalgae, fungi and bacteria to sense environmental conditions. (wikipedia.org)
  • The C-terminal domain is responsible for the attachment of the protein to the attachment of the protein to the cell wall. (wikipedia.org)
  • metal
  • Lippi M., Passerini A., Punta M., Frasconi P. (2012) Metal Binding in Proteins: Machine Learning Complements X-Ray Absorption Spectroscopy. (springer.com)
  • specific
  • Using specific monoclonal antibodies for Surfactant protein A, the protein can be detected in lung alveolar type II pneumocytes, Clara cells, and alveolar macrophages, but no extrapulmonary SP-A immunoreactivity was observed. (wikipedia.org)
  • Levels
  • When plasma transferrin levels rise, there is a reciprocal decrease in percent transferrin iron saturation, and a corresponding increase in total iron binding capacity in iron deficient states A decreased plasma transferrin can occur in iron overload diseases and protein malnutrition. (wikipedia.org)