Protein hepacivirus. Medical search. Frequent questions

The most crucial NS proteins are the viral helicase and the viral RNA-dependent RNA polymerase (RdRp) ( 2 ). (spandidos-publications.com)
Along this line, in both systems, release of nonstructural protein 3 (NS3) is essential for viral RNA replication. (uni-luebeck.de)
Understanding the interaction patterns between a particular virus and human proteins plays a crucial role in unveiling the underlying mechanism of viral infection and pathogenesis. (biomedcentral.com)
Studies have shown that different viral proteins may individually induce host responses to infection. (elsevierpure.com)
The recently developed HCV cell culture system, based on the JFH1 strain of HCV, has provided an opportunity to study the role of the viral proteins in the complete HCV replication cycle in human hepatoma cells. (monash.edu)
How the viral proteins functionally interact during replication of HCV in cell culture is not completely understood. (monash.edu)
Using this approach, the functional interactions of p7 and E2 with other viral proteins during HCV replication was investigated. (monash.edu)
Since the protein plays a critical role in the virus life cycle, chemical compounds that block p7 function are potential candidates for anti-viral therapy. (monash.edu)
To investigate the emergence of compensatory mutations in the viral proteins during cell passaging, the consensus sequences of the entire polyprotein coding regions of the wild type JFH1 and three chimeric viruses were determined. (monash.edu)
Aqil M, Naqvi AR, Bano AS, Jameel S (2013) The HIV-1 Nef protein binds argonaute-2 and functions as a viral suppressor of RNA interference. (virosin.org)
Availability http://carnot.utmb.edu/flavitrack Get in touch with ude.bmtu@niehcshc Supplementary details http://carnot.utmb.edu/flavitrack/B1S1.html 1 Launch Flaviviruses (FV) (+)-strand RNA infections in the same genus seeing that hepaci- and pestiviruses are in charge of many emerging individual encephalitic and hemorrhagic illnesses (Barrett and Higgs 2007 The ～10.5 kb genome encodes an individual polyprotein that's cleaved into 10 viral proteins (Fig. 1). (bioskinrevive.com)
HCV was first recognised in 1970 and described as non-A, non-B hepatitis, until 1989 when the pathogen was identified as hepatitis C. The structural proteins produced by Hepatitis C virus include the core protein and envelope glycoproteins E1 and E2, which are necessary for viral entry into host cells. (thenativeantigencompany.com)
Non-structural (NS) proteins include V7 viroporin and NS2 protease, which play essential roles in viral infection and replication, and NS3, NS4a, NS4b and NS5b proteins. (thenativeantigencompany.com)
OBJECTIVE: Direct-acting antivirals (DAAs) inhibit hepatitis C virus (HCV) infection by targeting viral proteins that play essential roles in the replication process. (itg.be)
A second project involves the Hepatitis C viral helicase NS3 (Non-Structural Protein 3). (uams.edu)
It involves an eleven amino acid subunit (HiBiT) tagged to a viral protein that creates measurable luminescence when incubated with the larger subunit (LgBiT). (purdue.edu)

Hepacivirus cross-species transmission and the origins of the hepatitis C virus" (PDF). (wikipedia.org)
The family Flaviviridae contains three genera of positive-strand RNA viruses, namely, Flavivirus, Hepacivirus (e.g., hepatitis C virus [HCV]), and Pestivirus. (uni-luebeck.de)
The hepatitis C virus (HCV) non-structural protein 3 (NS3) is essential for HCV maturation. (huji.ac.il)
We propose that the species Hepatitis C virus is renamed Hepacivirus C in order to acknowledge its unique historical position and so as to minimize confusion. (ox.ac.uk)
Erdtmann L, Franck N, Lerat H, Le Seyec J, Gilot D, Cannie I, Gripon P, Hibner U, Guguen-Guillouzo C (2003) The hepatitis C virus NS2 protein is an inhibitor of CIDE-B-induced apoptosis. (virosin.org)
Ji J, Glaser A, Wernli M, Berke JM, Moradpour D, Erb P (2008) Suppression of short interfering RNA-mediated gene silencing by the structural proteins of hepatitis C virus. (virosin.org)
The long-term evolution of the hepatitis C virus hypervariable region (HVR) and flanking regions of the E1 and E2 envelope proteins have been studied in a cohort of women infected from a common source of anti-D immunoglobulin. (ox.ac.uk)
Hepatitis C virus (HCV) is an enveloped, positive-sense, single stranded RNA virus and member of the hepacivirus genus of the family Flaviviridae . (thenativeantigencompany.com)
Our Hepatitis C antigen is a highly purified core protein for use in assay development, vaccine research and as antigens for the preparation of specific antibodies. (thenativeantigencompany.com)
We offer a large panel of monoclonal antibodies specific to Hepatitis C. Our Hepatitis C antibodies recognise a number of different HCV-specific proteins, including the core antigen, E1, E2 and NS3 - all of which may be used in a wide range of applications to study the biology of Hepatitis C. (thenativeantigencompany.com)
The 5' end of the NS-4 protein of different genotypes of hepatitis C virus (HCV) is highly variable in nucleotide and inferred amino acid sequence, with frequent predicted amino acid substitutions between all six of the major HCV genotypes described to date. (ox.ac.uk)
Subcellular localization of hepatitis C virus structural proteins in a cell culture system that efficiently replicates the virus. (ox.ac.uk)
The TWiV team speaks with Amit Kapoor and Ian Lipkin about how they discovered canine hepacivirus, and its implications for the origin and evolution of hepatitis C virus. (microbe.tv)

with previously reported NPHV se- der in the United States in 2011, ten- To phylogenetically analyze the virus quences from dogs and horses (Figure) tatively named canine hepacivirus ( 2 ), sequences based on an NS3 partial ( 2 , 6-8 ). (cdc.gov)
Hepacivirus A, or Canine hepacivirus (CHV) or Equine hepacivirus (EHV), is a positive-sense single-stranded RNA virus of the genus Hepacivirus. (wikipedia.org)
All other species are named according to the date of publication of a complete coding sequence with the exception of Hepacivirus B which includes GBV-B (providing a memorable link) and Hepacivirus A (canine hepacivirus/non-primate hepacivirus). (wikipedia.org)

3. Chimerical peptides according to the claim 1, characterized by containing a segment inhibitor of the activation of the NS3pro protease, this segment is a peptide able to bind to NS3pro and contact at least one residue comprised in the region Gly21-Lys28 of the N-terminal domain of the NS3pro protein from DV2 or structurally equivalent residues of the NS3pro protein from other Flavivirus. (patentsencyclopedia.com)
6. Chimerical peptides according to the claim 1, characterized by containing a segment inhibitor of the activation of the NS3pro protease which comprise the region Asp50-Glu62 of the NS2B protein from DV2 or the structurally equivalent segment of the protein NS2B from other Flavivirus, or an amino acid sequence showing a sequence similarity higher of equal to the 80% identity. (patentsencyclopedia.com)
Flavitrack will ultimately also contain buildings or 3D versions for any flavivirus proteins enabling combined series/structure evaluation to characterize common B- and T-cell epitopes account for Prp2 the functional effects of mutations and determine highly conserved areas. (bioskinrevive.com)
The family Flaviviridae includes important genera such as flavivirus and hepacivirus which comprise significant human pathogens that affect hundreds of millions annually. (purdue.edu)

Create 13 new species in the genus Hepacivirus and rename 1 species (family Flaviviridae)" (PDF). (wikipedia.org)
Based on its molecular genetic characteristics, the virus has been classified into the Hepacivirus genus of the family Flaviviridae. (monash.edu)
Proposed update to the taxonomy of the genera Hepacivirus and Pegivirus within the Flaviviridae family. (ox.ac.uk)
Proposals are described for the assignment of recently reported viruses, infecting rodents, bats and other mammalian species, to new species within the Hepacivirus and Pegivirus genera (family Flaviviridae). (ox.ac.uk)
10. Chimerical peptides according to the claim 1, where the mentioned virus from the Flaviviridae family is a Hepacivirus and specifically HCV. (patentsencyclopedia.com)
The findings from both the DENV and HCV studies provide a robust foundation for future tagging of viruses within the family Flaviviridae and offer insight on the structural proteins that compose the virion. (purdue.edu)

In this thesis, a chimeric JFH1 genome that encodes the p7 protein of genotype (GT) 1b strain J4 was generated. (monash.edu)

The translated polyprotein consists of three structural [capsid (C), membrane (M) and envelope (E)] and seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5). (spandidos-publications.com)
This allowed us to reinvestigate the subcellular localization of HCV structural proteins in the context of an infectious cycle. (ox.ac.uk)
In agreement with previous reports, confocal immunofluorescence analysis of the subcellular localization of HCV structural proteins indicated that, in infected cells, the glycoprotein heterodimer is retained in the endoplasmic reticulum. (ox.ac.uk)
In conclusion, the cell culture system for HCV allowed us for the first time to characterize the subcellular localization of HCV structural proteins in the context an infectious cycle. (ox.ac.uk)
Luminescent reporter tags have yet to be incorporated into the structural proteins of dengue virus (DENV) without significantly affecting replication or infectivity and successful tagging would allow for targeted studies examining access to specific structural epitopes. (purdue.edu)
Engineering tags in DENV structural proteins is particularly difficult because most reporter tags involve large insertions which may create steric hindrance and inhibit proper protein folding. (purdue.edu)

Using the structure of the virion as a guide, the HiBiT reporter tag was incorporated into the structural region of the DENV genome including sites in capsid (C) as well as the glycoproteins membrane (M) and envelope (E). Resulting recombinant viruses were characterized and tag sites within the C protein membrane anchor as well as the transmembrane domain of M protein were found to tolerate HiBiT insertion and produce infectious particles. (purdue.edu)

However, the task of predicting protein-protein interactions between a new virus and human cells is extremely challenging due to scarce data on virus-human interactions and fast mutation rates of most viruses. (biomedcentral.com)
In this work, we cast the problem of predicting virus-human protein interactions as a binary classification problem and focus specifically on emerging viruses that has limited experimentally verified interaction data. (biomedcentral.com)
there is overlap between the two genera in the type of internal ribosomal entry site and the presence of miR-122 sites in the 5' UTR, the predicted number of N-linked glycosylation sites in the envelope E1 and E2 proteins, the presence of poly U tracts in the 3' UTR and the propensity of viruses to establish a persistent infection. (ox.ac.uk)

8. Chimerical peptides according to the claim 1, characterized by containing a segment inhibitor of the activation of the NS3pro protease which comprise the region Thr20-Leu31 of the NS4A protein from HCV or the structurally equivalent segment of the protein NS4A from other Hepacivirus, or an amino acid sequence showing a sequence similarity higher of equal to the 80% identity. (patentsencyclopedia.com)

2004 multiple alignment editor are provided for those polyproteins in Flavitrack (presently 544 sequences) a synopsis subset of 49 specific FV and one which combines all of the FV pestivirus and hepacivirus polyproteins in the NCBI (923 sequences). (bioskinrevive.com)

RNA-protein interactions are central to biological regulation. (nih.gov)
Additionally, we employ an additional objective which aims to maximize the probability of observing human protein-protein interactions. (biomedcentral.com)
Virus infection involves several types of protein-protein interactions (PPIs) between the virus and its host. (biomedcentral.com)
We screened over 2800 membrane proteins for interaction with NS3 and 90 previously unknown interactions were identified. (huji.ac.il)
The newly discovered protein interactions may provide insights into HCV infection mechanisms and potentially provide new therapeutic targets against HCV. (huji.ac.il)
In summary, this thesis demonstrates the importance of functional interactions between HCV proteins during virus morphogenesis and infectivity. (monash.edu)
We are studying the mechanism of NS3 as well as its interactions with other HCV and cellular proteins. (uams.edu)
Our research projects are currently expanding in development of new tools for studying and protein-protein interactions and post-translational modifications at specific sites in the genome using a Crispr-based approach coupled with protein mass spectrometry. (uams.edu)
Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. (uchicago.edu)

Sequencing analysis revealed the selection of a single adaptive mutation in the HCV E2 envelope protein that was largely responsible for the variant phenotype. (nih.gov)

Results: HCV envelope protein E2 induced strong inflammatory responses in human thyrocytes, resulting in production of interleukin (IL)-8, IL-6, and tumor necrosis factor-a. (elsevierpure.com)

The protein is membrane associated and thus probably interacts with other membrane proteins. (huji.ac.il)
However, the vast majority of known NS3 host partners are soluble proteins rather than membrane proteins, most likely due to lack of appropriate platforms for their discovery. (huji.ac.il)
Utilization of an integrated microfluidics platform enables analysis of membrane proteins in their native form. (huji.ac.il)
p7 is a highly hydrophobic, integral membrane protein and is classified in the viroporin family. (monash.edu)
Fig. 1 Polyprotein common to FV with favored abbreviations for the structural [capsid (C) membrane (M) and envelope (E)] and non-structural (NS) proteins whose function is definitely mentioned where known. (bioskinrevive.com)

5. Chimerical peptides according to the claim 1, where the mentioned segment inhibitor of the activation of the NS3pro protease contact at least one residue comprised in the region Glu32-Thr38 of the N-terminal domain of the NS3pro protein from HCV or structurally equivalent residues of the NS3pro protein from other Hepacivirus. (patentsencyclopedia.com)

Assignments into 14 Hepacivirus species (Hepacivirus A-N) and 11 Pegivirus species (Pegivirus A-K) are based on phylogenetic relationships and sequence distances between conserved regions extracted from complete coding sequences for members of each proposed taxon. (ox.ac.uk)

Serological methods to detect HCV IgM antibodies in patient's serum are reported to be unreliable but diagnostic methods to detect HCV total antibody and HCV core protein may have some value. (thenativeantigencompany.com)
HCV core protein, which was detectable in the plasma of infected patients, reduced TLR9-triggered IFN-alpha and increased TNF-alpha and IL-10 production in PBMCs but not in isolated PDCs, suggesting HCV core induced PDC defects. (umassmed.edu)
In conclusion, our results suggest that HCV interferes with antiviral immunity through TLR2-mediated monocyte activation triggered by the HCV core protein to induce cytokines that in turn lead to PDC apoptosis and inhibit IFN-alpha production. (umassmed.edu)

Interestingly, a case of evolution toward the prototype sequence was observed during chronic infection, possibly because the helicase activity of the protein containing the Y1444F substitution is reduced compared to the prototype sequence. (ox.ac.uk)

It encodes two envelope proteins (E1 and E2) as well as cysteine and serine proteases. (wikipedia.org)

The excised DNA quadruplexes can bind to proteins such as DHX36 (a helicase), leading to formation of sub-organelles called stress granules. (uams.edu)

Conclusion: Our data support a key role for HCV envelope protein E2 in triggering thyroid autoimmunity through activation of cytokine pathways by bystander mechanisms. (elsevierpure.com)
HIV Envelope Protein gp41" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (musc.edu)
Transmembrane envelope protein of the HUMAN IMMUNODEFICIENCY VIRUS which is encoded by the HIV env gene. (musc.edu)
This graph shows the total number of publications written about "HIV Envelope Protein gp41" by people in this website by year, and whether "HIV Envelope Protein gp41" was a major or minor topic of these publications. (musc.edu)
Below are the most recent publications written about "HIV Envelope Protein gp41" by people in Profiles. (musc.edu)

Every year, 3-4 million Reagent (Invitrogen, Carlsbad, CA, Until now, it has been impossible persons become infected with HCV, USA), and a target sequence in the to associate Hepacivirus infection in and 350,000 die of this infection ( 1 ). (cdc.gov)

We developed a multitask transfer learning approach that exploits the information of around 24 million protein sequences and the interaction patterns from the human interactome to counter the problem of small training datasets. (biomedcentral.com)
Instead of using hand-crafted protein features, we utilize statistically rich protein representations learned by a deep language modeling approach from a massive source of protein sequences. (biomedcentral.com)
2 FEATURES The major features of Flavitrack are sequence retrieval BLAST assessment of a given RNA or protein sequence to the people in the database and PHA-739358 sequence alignment of user selected sequences. (bioskinrevive.com)

However, experimental approaches to unravel PPIs are limited by several factors, including the cost and time required, the generation, cultivation and purification of appropriate virus strains, the availability of recombinantly expressed proteins, generation of knock in or overexpression cell lines, availability of antibodies and cellular model systems. (biomedcentral.com)

Surprisingly, no colocalization was observed between the glycoprotein heterodimer and the capsid protein in infected cells. (ox.ac.uk)

Cross-linking immunoprecipitation (CLIP)-seq is a powerful tool for genome-wide interrogation of RNA-protein interactomes, but current CLIP methods are limited by challenging biochemical steps and fail to detect many classes of noncoding and nonhuman RNAs. (nih.gov)
Over the past decade, genome-wide assays and new imaging approaches have enabled a greater understanding of how access to the genome is regulated by nucleosomes and associated proteins. (rockefeller.edu)

It is predicted to have a polyprotein that can be cleaved into 10 smaller proteins. (wikipedia.org)
The latter region of NS proteins, at the C-terminal part of the polyprotein, has a great contribution in the RNA replication process. (spandidos-publications.com)

Variations are used to evaluate interplay between proteins and other molecules. (uchicago.edu)

The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. (cancerindex.org)

FAST-iCLIP of Poly-C binding protein 2 (PCBP2) showed that PCBP2-bound CU-rich motifs in different topologies to recognize mRNAs and noncoding RNAs with distinct biological functions. (nih.gov)
The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. (cancerindex.org)
We hypothesized that HCV E2 protein binding to CD81 expressed on thyroid cells activates a cascade of inflammatory responses that can trigger autoimmune thyroiditis in susceptible individuals. (elsevierpure.com)
Conclusion: Increased CCAAT/enhancer-binding protein β (C/EBPβ) and nonstructural component 5A (NS5A) are essential components for increased gluconeogenesis. (ttu.edu)

These results show that FAST-iCLIP can be used to rapidly discover and decipher mechanisms of RNA-protein recognition across the diversity of human and pathogen RNAs. (nih.gov)

Recent studies suggest that T-cell immune pressure is an important factor in the evolution of the nonstructural proteins in HCV. (ox.ac.uk)

Fabozzi G, Nabel CS, Dolan MA, Sullivan NJ (2011) Ebolavirus proteins suppress the effects of small interfering RNA by direct interaction with the mammalian RNA interference pathway. (virosin.org)

Scope includes mutations and abnormal protein expression. (cancerindex.org)

In this study, we screened all the nonstructural proteins of HCV and found that HCV NS2 could suppress RNAi induced either by small hairpin RNAs (shRNAs) or small interfering RNAs (siRNAs) in mammalian cells. (virosin.org)

Our goal is to recapitulate RNA replication in vitro using biological relevant substrates and proteins and a biologically relevant sub-genomic replicon of HCV. (uams.edu)

Of these, several proteins were selected for validation by co-immunoprecipitation and for NS3 proteolytic activity. (huji.ac.il)

Viral16

Hepatitis13

Canine hepacivirus3

Flavivirus4

Flaviviridae6

Genotype1

Structural proteins6

Glycoproteins1

Viruses3

NS4A1

Pestivirus1

Interactions9

Mutation1

Tumor1

Membrane5

Protease1

Pegivirus1

Core Protein3

Chronic1

Encodes1

Helicase1

Envelope protein5

Infection1

Sequences3

Antibodies1

Glycoprotein1

Genome2

Polyprotein2

Molecules1

Receptors1

Binding4

Mechanisms1

Evolution1

Interference1

Mutations1

Cells1

Biological1

Proteolytic activity1