• Vascular endothelial cells, as a monolayer in direct contact with the flowing blood, bear the most of the wall shear stresses and have important homeostatic functions in response to stress [16] . (plos.org)
  • Eicosapentaenoic Acid: Modulated Platelet Activation and Thrombosis Proteins in Vascular Endothelial Cells During Inf. (cardiologytalk.com)
  • The title of my presentation that was given there is Eicosapentaenoic Acid or EPA modulates the expression or proteins linked to platelet activation and thrombosis in vascular endothelial cells during inflammation. (cardiologytalk.com)
  • Among them, VEGF-A can promote the division and proliferation of vascular endothelial cells and neovascularization and maintain the survival of new vessels. (molvis.org)
  • LINC00926 promotes pyroptosis of hypoxia-induced human umbilical vein vascular endothelial cells by recruiting ELAVL1]. (nih.gov)
  • We demonstrate that UCN-01, at concentrations lower than those necessary to inhibit cancer cell growth, inhibit proliferation of human endothelial cells in vitro. (karger.com)
  • We conclude that UCN-01, at clinically relevant concentrations, exerts an anti-neovascularization effect by blocking two important steps in vessel formation: (1) the response of cancer cells to hypoxia, and (2) endothelial cell proliferation. (karger.com)
  • In addition, at 48H, BPs linked to inflammatory phenotypes were activated (confirming KEGG results), while BPs linked to extracellular matrix (ECM) structural organisation, endothelial cell proliferation, and collagen metabolism were repressed. (nature.com)
  • Low PDCD4 level was associated with reduced proliferation but not apoptosis or phosphorylation of endothelial nitric oxide synthase caused by pulsatile shear stress to help maintain the homeostasis of endothelial cells. (plos.org)
  • Ample evidence shows that pro-atherosclerotic disturbed flow induces sustained activation of atherogenic genes in endothelial cells to promote their oxidation, inflammation, cell cycle progression and proliferation, whereas pulsatile shear stress tends to maintain endothelial cells in a quiescent and less proliferative state with a low level of oxidation and inflammation [16] , [17] . (plos.org)
  • Endothelial cell lineage tracing showed that BNP directly stimulated the proliferation of resident endothelial cells via NPR-A binding and p38 MAP kinase activation. (elifesciences.org)
  • BNP also stimulated the proliferation of WT1 + epicardium-derived cells but only in the hypoxic area of infarcted hearts. (elifesciences.org)
  • The origin of new endothelial cells (i.e. resident or infiltrating) as well as the underlying mechanism leading to their proliferation (partial endothelial-to-mesenchymal transition [EndMT] or not) have long been debated. (elifesciences.org)
  • Characterization of protein kinase C beta isoform's action on retinoblastoma protein phosphorylation, vascular endothelial growth factor-induced endothelial cell proliferation, and retinal neovascularization. (bvsalud.org)
  • The potential functional consequences of PKC beta-induced retinoblastoma phosphorylation could include enhanced E2 promoter binding factor transcriptional activity and increased VEGF -induced endothelial cell proliferation. (bvsalud.org)
  • The proliferation of choroidal endothelial cells (CECs) is one important step in the formation of new vessels. (molvis.org)
  • Transcriptional coactivator Yes-associated protein (YAP) can promote the proliferation of multiple cancer cells, corneal endothelial cells, and vascular smooth muscle cells, which participate in angiogenesis. (molvis.org)
  • This study showed that YAP upregulation promoted CNV formation by upregulating the proliferation of endothelial cells, providing evidence for the molecular mechanisms of CNV and suggesting a novel molecular target for nAMD treatment. (molvis.org)
  • Hyaline membranes appear along with a proliferation of type 2 alveolar lining cells. (medscape.com)
  • Thalidomide and bevacizumab, both VEGF inhibitors, were tested for their ability to prevent the formation of circular networks and proliferation of CD31⁺ endothelial AVM cells. (researchgate.net)
  • Thalidomide and bevacizumab reduced endothelial AVM cell proliferation. (researchgate.net)
  • The monoclonal VEGF antibody bevacizumab alleviates this effect, prevents circular network formation and proliferation of AVM endothelial cells in vitro. (researchgate.net)
  • 4 In addition, vascular endothelial growth factor (VEGF) is an angiogenic factor that regulates endothelial survival, proliferation, differentiation, and vascular permeability, and has been shown to be an important candidate for cancer therapy as it is upregulated in many tumors. (beckman.com)
  • The aim of the present study was to identify whether P. gingivalis heat‑shock protein 60 (HSP60) induced the dysfunction of human umbilical vein endothelial cells (HUVECs) in vitro. (spandidos-publications.com)
  • Endothelial dysfunction is a critical initiating factor contributing to cardiovascular diseases, involving the gut microbiome-derived metabolite trimethylamine N-oxide (TMAO). (nature.com)
  • This study aims to clarify the time-dependent molecular pathways by which TMAO mediates endothelial dysfunction through transcriptomics and metabolomics analyses in human microvascular endothelial cells (HMEC-1). (nature.com)
  • The discrepancies between studies on TMAO-induced endothelial dysfunction may be attributed to different cell types and animal models used, including different metabolic backgrounds, as well as to distinct TMAO treatment concentrations and durations. (nature.com)
  • Endothelial dysfunction during diabetes has been previously reported to be at least in part attributed to increased oxidized low‑density lipoprotein (oxLDL) levels mediated by high glucose (HG) levels. (spandidos-publications.com)
  • These results suggest that rosmarinic acid can exert a protective effect against oxLDL‑mediated endothelial dysfunction under HG conditions by reducing the interaction between monocytes and ECs in addition to preventing monocyte diapedesis. (spandidos-publications.com)
  • A number of studies have previously suggested that vascular endothelial dysfunction caused by increased junctional permeability is a key event in the development and progression of atherosclerosis and atherosclerotic cardiovascular diseases ( 3 , 4 ). (spandidos-publications.com)
  • Chronic metabolic disorders, such as hyperglycemia and dyslipidemia, promote endothelial dysfunction and eventually leakage, resulting in increased monocyte diapedesis ( 5 - 7 ). (spandidos-publications.com)
  • Endothelial dysfunction during diabetes is closely associated with increased oxidized low-density lipoprotein (oxLDL) levels mediated by high glucose (HG) conditions ( 8 ). (spandidos-publications.com)
  • BMP4 stimulates production of reactive oxygen species (ROS) and causes endothelial cell dysfunction. (houstonmethodist.org)
  • Oxidative stress can denature lipids and proteins [ 10 , 12 - 14 ], as well as induce DNA and RNA fragmentation [ 15 - 17 ], leading to cell dysfunction, injury, and death. (hindawi.com)
  • Conditioned media from CBox treated macrophage induced greater endothelial dysfunction (monolayer permeability and inhibition of wound healing). (cdc.gov)
  • The impaired homeostasis and platelet dysfunction implicate alterations in the protein C pathway, which might contribute to the loss of endothelial barrier function in fatal infections. (cdc.gov)
  • Janowska J, Chudek J, Olszanecka-Glinianowicz M, Semik-Grabarczyk E, Zahorska-Markiewicz B. Interdependencies among Selected Pro-Inflammatory Markers of Endothelial Dysfunction, C-Peptide, Anti-Inflammatory Interleukin-10 and Glucose Metabolism Disturbance in Obese Women. (medsci.org)
  • We have studied the relationship between clinical and biochemical parameters and C-peptide and anti-inflammatory IL-10, as well as selected markers of inflammation and endothelial dysfunction such as: CCL2, CRP, sICAM-1, sVCAM-1 and E-selectin in obese women with various degree of glucose metabolism disturbance. (medsci.org)
  • Disturbed lipid and carbohydrate metabolism are manifested by enhanced inflammation and endothelial dysfunction in patients with simply obesity. (medsci.org)
  • Since endothelial dysfunction precedes overt atherosclerosis, investigating the ability of drugs to activate cytoprotective signalling pathways that prevent or reverse endothelial dysfunction is an attractive research strategy. (bmj.com)
  • LINC00926 is involved in hypoxia-induced vascular endothelial cell dysfunction via miR-3194-5p regulating JAK1/STAT3 signaling pathway. (nih.gov)
  • Qin R , Zhu H , Wang F , Song M , Lin P , Xing Y , Zhang W , Zhong M , Wang Z , . Platelet activation in diabetic mice models: the role of vascular endothelial cell-derived protein disulfide isomerase-mediated GP IIb/IIIa receptor activation. (aging-us.com)
  • Upon stimulation of these cells, P-selectin is translocated to the plasma membrane where it functions as a receptor for monocytes and neutrophils. (rupress.org)
  • Proteins that do not bind the receptor are directed to lysosomal degradation. (nature.com)
  • Endothelial cells express both PKD1 (polycystin-1), an eleven transmembrane domain protein, and PKD2 (polycystin-2), a transient receptor potential (TRP) channel. (grantome.com)
  • In this study, the effects of JTE-013, a specific antagonist of the migration-inhibitory receptor EDG-5, on Sph-1-P-elicited responses were examined in human umbilical vein endothelial cells (HUVECs) and vascular smooth muscle cells (SMCs), which expressed EDG-5 protein weakly and abundantly, respectively. (nih.gov)
  • Endothelial protein C receptor (EPCR) also known as activated protein C receptor (APC receptor) is a protein that in humans is encoded by the PROCR gene. (wikipedia.org)
  • The protein encoded by this gene is a receptor for protein C that enhances its activation. (wikipedia.org)
  • The protein is also involved in Plasmodium falciparum malaria as subtypes of the Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family use EPCR of the host as a receptor. (wikipedia.org)
  • accepted 20 January 2014) Increasing evidence has demonstrated that beyond its role in activation of protein C, endothelial cell protein C receptor (EPCR) is involved in vascular inflammation. (sagepub.com)
  • In vitro studies have reported a dramatic increase in PC anticoagulant pathway is the endothelial cell protein C EPCR shedding from the endothelium by a wide variety receptor (EPCR). (sagepub.com)
  • P-selectin, soluble endothelial protein C receptor, soluble thrombomodulin, plasminogen activator inhibitor 1, ADAMTS-13, von Willebrand factor, tissue factor, soluble intercellular adhesion molecule 1, and vascular cell adhesion molecule 1 were more elevated in LF patients than in controls. (cdc.gov)
  • Endothelial protein C receptor, thrombomodulin, intercellular adhesion molecule 1, plasminogen activator inhibitor 1, D-dimer, and hepatocyte growth factor were higher in fatal than nonfatal LF cases. (cdc.gov)
  • This receptor may contribute to the accumulation and activation of eosinophils and other inflammatory cells in the allergic airway. (cancerindex.org)
  • Advanced glycation endproducts interacting with their endothelial receptor induce expression of vascular cell adhesion molecule-1 (VCAM-1) in cultured human endothelial cells and in mice. (jci.org)
  • In view of the accelerated vascular disease observed in patients with diabetes, and the enhanced expression of VCAM-1 in diabetic rabbits, we examined whether irreversible advanced glycation endproducts (AGEs), could mediate VCAM-1 expression by interacting with their endothelial cell receptor (receptor for AGE, RAGE). (jci.org)
  • APC inhibits ET-induced TNF-α production in vitro in human monocytes by inhibiting activation of NFkB and AP-1 by inhibiting degradation of IkB and mitogen-activated protein kinase pathways, respectively. (eurekaselect.com)
  • To study the migration of microglia across the BBB, we developed an in vitro co-culture system of mouse brain endothelial cells (MBECs) and Ra2 microglia using Transwell inserts. (portlandpress.com)
  • This was an in vitro laboratory study, so we had very well controlled conditions to study the effects of inflammation on these cells. (cardiologytalk.com)
  • Pharmacological inhibitor studies confirmed an oxidant/MAPK/NFkb dependent pro-inflammatory response (gene and protein expression of CXCL9, 10, 11, TNF-a, IL-6) in mice and in in vitro exposed macrophages. (cdc.gov)
  • We have established a method allowing to study the cell-specific secretome of pericytes in vitro-using Turbo-ID, a mutant non-toxic biotin ligase (6). (lu.se)
  • The levels of vascular endothelial (VE)‑cadherin, endothelial nitric oxide synthase (eNOS) and cysteinyl aspartate‑specific protease‑3 (caspase‑3) were measured using western blot analysis. (spandidos-publications.com)
  • It was hypothesized that black currant fruit extracts would cause activation of endothelial nitric oxide synthase (eNOS) through activation of redox-sensitive PI3 kinase/Akt signaling pathway. (nih.gov)
  • Activated SIRT1 signals hypoxia inducible factor (HIF)-2α, which transactivates vascular endothelial growth factor (VEGF) and erythropoietin. (molvis.org)
  • However, its role has not been evaluated in hypoxic choroidal endothelial cells (CECs), the precursor of VEGF-mediated choroidal angiogenesis. (molvis.org)
  • Activation of protein kinase C (PKC) enhances the angiogenic process and is involved in the signaling of vascular endothelial growth factor ( VEGF ). (bvsalud.org)
  • The mitogenic action of VEGF , a potent hypoxia -induced angiogenic factor , was increased by 2-fold in retinal endothelial cells by the overexpression of PKC beta 1 or beta 2 isoforms and inhibited significantly by the overexpression of a dominant-negative PKC beta 2 isoform but not by the expression of PKC alpha, delta , and zeta isoforms . (bvsalud.org)
  • Association of PKC beta 2 isoform with retinoblastoma protein was discovered in retinal endothelial cells , and PKC beta 2 isoform increased retinoblastoma phosphorylation under basal and VEGF -stimulated conditions. (bvsalud.org)
  • Purpose: Vascular Endothelial Growth Factor (VEGF) promotes angiogenesis in many different tumor types. (johnshopkins.edu)
  • This study assesses the prognostic value of VEGF protein levels in a cohort of patients with oral and oropharyngeal squamous cell carcinomas. (johnshopkins.edu)
  • VEGF protein levels were determined using immunohistochemistry. (johnshopkins.edu)
  • YAP expression increased following laser exposure, in accordance with vascular endothelial growth factor (VEGF) expression. (molvis.org)
  • In response to mechanical stress, VEGF gene and protein expression increased in patient AVM endothelial cells. (researchgate.net)
  • Bevacizumab inhibited circular network formation of endothelial AVM cells and lowered VEGF gene and protein expression, even though the cells were exposed to mechanical stress. (researchgate.net)
  • Overall, mechanical stress increases VEGF expression in the microenvironment of AVM cells. (researchgate.net)
  • Graphical abstract Mechanical stress increases VEGF expression in endothelial AVM cells, possibly causing the VEGF upregulation in the microenvironment of AVM cells. (researchgate.net)
  • UCN-01, a protein kinase C (PKC) inhibitor, induces growth arrest and apoptosis in cancer cells and was recently introduced in a phase I clinical trial. (karger.com)
  • Polyphenols are known to induce vasodilatory function via activation of the redox-sensitive phosphatidylinositol-3 (PI3)/protein kinase B (Akt) pathway. (nih.gov)
  • Cells were also treated with PKC activator PEP005 or specific siRNA against protein kinase Cδ (PKCδ). (medscimonit.com)
  • MTX increases intracellular accumulation of adenosine monophosphate (AMP) and 5-aminoimidazole-4-carboxamide ribonucleotide which activates AMP-activated protein kinase (AMPK). (bmj.com)
  • The tyrosine kinase receptors Tyro3, Axl and Mer (TAMs) and their ligands protein S and Gas6 are involved in the uptake of PS-exposing apoptotic cells in macrophages and dendritic cells. (lu.se)
  • P-selectin, a granule membrane protein of platelets and endothelial cells, follows the regulated secretory pathway in AtT-20 cells. (rupress.org)
  • In contrast, the surface expression of P-selectin transfected into CHO cells, which do not have a regulated pathway of secretion, did not change with 8-Br-cAMP treatment. (rupress.org)
  • Prolonged ER stress-mediated cell death, occurring via the intrinsic apoptotic signaling pathway, therefore might be associated with the pathogenesis of FECD. (cardiff.ac.uk)
  • This was found to be dependent on the reactive oxygen species (ROS)/p38 MAPK/forkhead box O1 (FOXO1)/thioredoxin interacting protein (TXNIP) signaling pathway. (spandidos-publications.com)
  • Protein C is an anti-coagulant serine protease activated by the blood coagulation pathway. (wikipedia.org)
  • vascular inflammation Introduction sEPCR circulates in plasma, retaining its ability to bind both PC and APC, but does not enhance protein C The protein C (PC) anticoagulant pathway plays an activation (Kurosawa et al. (sagepub.com)
  • Examination of the blue module exposed the presence of a large number of molecular chaperones (DNAJA1 DNAJA4 DNAJB1 DNAJB6 and DNAJB9) and warmth shock proteins (HSPA1A HSPA1B and HSPH1) many of which are known to be induced by ER stress as part of the UPR pathway (Table 1) (18). (careersfromscience.org)
  • We present a rate equation model for the TGF-b pathway in endothelial cells together with novel measurements. (lu.se)
  • Since hypoxia activates hypoxia-inducible factor (HIF-1)-dependent transcription in cancer cells that, in a paracrine fashion, drive tumor angiogenesis, we investigated the effects of UCN-01 on HIF-1-responsive promoter constructs. (karger.com)
  • These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)
  • This chapter provides an overview of the key role of hEPC in promoting angiogenesis and their potential use for cell therapy. (intechopen.com)
  • It acts by inducing angiogenesis and endothelial cell migration. (bvsalud.org)
  • The addition of microglia or ATP led to the disruption of the MBEC monolayer and significantly decreased barrier function as measured by trans-endothelial electrical resistance (TEER) and electric cell-substrate impedance sensing (ECIS). (portlandpress.com)
  • In conclusion, we offer evidence that microglia migration across the brain endothelial cell monolayer is increased in the presence of ATP in a manner that involves MMP secretion. (portlandpress.com)
  • Subsequently, rosmarinic acid treatment significantly reversed oxLDL‑induced overexpression of adhesion molecules and monocyte‑EC adhesion, oxLDL‑induced endothelial junction hyperpermeability and monocyte transmigration through the endothelial monolayer under HG conditions, in a dose‑dependent manner. (spandidos-publications.com)
  • Endothelial cells (ECs) form a semi-permeable monolayer that separates the bloodstream from underlying tissues and regulate the infiltration of blood cells and proteins through the vessel wall ( 1 , 2 ). (spandidos-publications.com)
  • Exposure of cultured human ECs to AGEs induced expression of VCAM-1, increased adhesivity of the monolayer for Molt-4 cells, and was associated with increased levels of VCAM-1 transcripts. (jci.org)
  • This monolayer of cells acts as a barrier to separate the vascular lumen from surrounding tissues and regulates the passage of fluid and nutrients between the two. (beckman.com)
  • This monolayer of cells is an important endocrine organ, and has been shown to play a major role in regulating blood fluidity, the transfer of solutes and macromolecules, immune responses and inflammation. (beckman.com)
  • Flow cytometry was used to detect cell apoptosis. (medscimonit.com)
  • PKCδ-siRNA transfection increased cell viabilities and reduced cell apoptosis in HAECs exposed to H/R. Baicalin treatment preserved cell viabilities and reduced apoptosis of H/R-exposed HAECs in a concentration- dependent manner. (medscimonit.com)
  • Baicalin treatment reduced phosphorylation levels of PKCδ and p53, as well as the expression levels of active caspase3 and bax in HAECs exposed to H/R. The treatment of PKC activator PEP005 impaired the protective effects of baicalin in increasing cell viabilities and reducing apoptosis in HAECs exposed to H/R. (medscimonit.com)
  • Baicalin exerts vascular a protective effect on HAECs exposed to H/R by reducing cell apoptosis. (medscimonit.com)
  • Apoptosis was assessed by flow cytometry to detect Annexin-V positive cells, and terminal deoxynucleotidyl transferase dUTP nick end (TUNEL) labeling. (houstonmethodist.org)
  • DENV infection of endothelial cells may cause cell apoptosis or vascular leakage and result in clinical illness of hemorrhage. (tmu.edu.tw)
  • Additionally, P. gingivalis induces macrophage foam cell formation ( 11 ) and stimulates oxidation of low-density lipoprotein ( 12 ). (spandidos-publications.com)
  • TMAO treatment for either 24H or 48H induces reduced cell viability and enhanced oxidative stress. (nature.com)
  • APC regulates the coagulation system by inactivating the activated form of factors V and VIII in the presence of protein S. Tumor necrosis factor-α(TNF-α) plays critical roles in the development of disseminated intravascular coagulation, acute respiratory distress syndrome and shock in sepsis by inducing endothelial cell damage through activation of neutrophils. (eurekaselect.com)
  • The purpose of this study was to investigate whether unfolded proteins accumulate in the corneal endothelium in FECD and if they are involved in triggering cell death. (cardiff.ac.uk)
  • Endothelial inflammation increases the adhesiveness of monocytes to the endothelium in addition to increasing vascular permeability, promoting diabetic atherogenesis. (spandidos-publications.com)
  • Acutely, hemin sensitizes endothelial cells to oxidants but chronically protects the endothelium through the induction of ferritin. (umn.edu)
  • EPCR is a type I transmembrane of inflammatory mediators (IL-1β,H O , and phorbol protein, which is highly expressed on the endothelium of 2 2 myristate acetate) and thrombin, and EPCR shedding is large vessels, while it is present at trace levels in most potentiated by the microtubule disrupting agent, nocoda- capillary beds (Mosnier et al. (sagepub.com)
  • This injury appears to be a consequence of the host's immunological response to viral antigens that have penetrated the endothelium by means of the cells' own integrins. (medscape.com)
  • Endothelial cells (ECs) form the endothelium, a layer of single-cell thickness that coats the interior wall of all blood vessels including arteries, capillaries, and veins. (beckman.com)
  • The vascular endothelium is formed by a sheet of endothelial cells tethered together by junctional proteins such as tight and adherens junctions. (bvsalud.org)
  • One complementary therapy could be 're-activating' vasculogenesis (i.e. the differentiation of precursor cells into mature endothelial cells), a mechanism that occurs in the heart during development but is quiescent in adult hearts. (elifesciences.org)
  • Choroidal endothelial cells (RF/6A) were maintained in a semiconfluent state and hypoxia was induced by exposing the cells to cobalt chloride for 24 h. (molvis.org)
  • Endothelial cells regulate arterial contractility. (grantome.com)
  • We will investigate the novel hypothesis that PKD1 and PKD2 proteins regulate arterial contractility, investigate signaling mechanisms involved and test the concept that vasodilation mediated through these pathways is dysfunctional during hypertension. (grantome.com)
  • Cultured human arterial endothelial cells (HAECs) were exposed to hypoxia/deoxygenation (H/R). Cells were also treated with baicalin at serially diluted concentrations. (medscimonit.com)
  • Studies of human umbilical vein endothelial cells (HUVEC) and arterial endothelial cells (HAEC) showed that therapeutically relevant concentrations of MTX phosphorylate AMPKα Thr172 , and induce cytoprotective genes including manganese superoxide dismutase (MnSOD) and haem oxygenase-1 (HO-1). (bmj.com)
  • Primordial endothelial cells specialize into tissue-specific phenotypes of arterial, venous, hemogenic, and lymphatic subtypes with distinct functions for vascular development. (beckman.com)
  • Regulatory mechanisms, physiological functions and pathological involvement during hypertension of PKD1 protein and PKD2 channels in endothelial cells are unclear. (grantome.com)
  • Aim 1 will test the hypothesis that endothelial-dependent physiological stimuli activate PKD1/PKD2 coupling in endothelial cells, leading to vasodilation. (grantome.com)
  • Under physiological circumstances, the resistance of the endothelial cell lining to interactions with platelets and coagulation factors prevents thrombosis. (medscape.com)
  • As bioactive compounds tend to interact with multiple proteins with various physiological effect, we aimed to identify other potential shared targets to understand the extent in which these chemicals could lead to GWI. (cdc.gov)
  • We recently found low level of tumor suppressor programmed cell death 4 (PDCD4) associated with reduced atherosclerotic plaque area (unpublished). (plos.org)
  • Programmed cell death 4 (PDCD4) is an important tumor suppressor in the development of various human cancers [1] and inhibits translation rather than transcription. (plos.org)
  • EPCR can be shed from the cell surface, which is mediated by tumor necrosis factor-α converting enzyme (TACE). (sagepub.com)
  • William Kaelin, Jr. was in 1995 engaged in the study of the von Hippel-Lindau tumor suppressor gene, and after isolation of the first full-length clone of the gene showed that it could suppress tumor growth in VHL mutant tumorigenic cell lines. (nobelprize.org)
  • We report that, in addition to direct inhibitory effects on endothelial cell growth, UCN-01 abrogates hypoxia-mediated transactivation of HIF-1-responsive promoters in a prostate cancer cell line. (karger.com)
  • Induction of hypoxia was confirmed by flow cytometric analysis and the levels of SIRT1 were noted in a hypoxic condition as well in the cells after blocking SIRT1 activity using sirtinol. (molvis.org)
  • Hypoxia of CEC was induced by exposing the cells to cobalt chloride (Sigma-Aldrich, St. Louis MO) in serum media, as described previously [ 12 , 13 ], and the induction was confirmed by cytotoxicity and cell cycle analysis. (molvis.org)
  • A circulating angiopoietin-like protein that is expressed in a variety of tissues in response to HYPOXIA. (bvsalud.org)
  • However, changes in cell signalling in response to hypoxia that are mediated by RGS5 stroke. (lu.se)
  • Enstrom A, Carlsson R, Ozen I, and Paul G. RGS5: a novel role as a hypoxia-responsive protein that suppresses chemokinetic and chemotactic migration in brain pericytes. (lu.se)
  • 2000). In addition, phosphorylation of endothelial cell surface, where it enhances the rate of PC p38MAPK, ERK1/2, and JNK was increased by stimula- activation (Mosnier et al. (sagepub.com)
  • Aim 3 will study the hypothesis that hypertension is associated with pathological alterations in PKD1/PKD2 channel signaling in endothelial cells that inhibits vasodilation mediated by these proteins. (grantome.com)
  • We display that several recognized modules are significantly enriched in genes for known pathways and confirm a module enriched for unfolded protein response (UPR) genes using siRNA and the UPR inducer tunicamycin. (careersfromscience.org)
  • This imbalance has at least two direct consequences: damage to individual components of the living cell and induction of inflammatory pathways that further perpetuate the damage. (hindawi.com)
  • What pathways are this gene/protein implicaed in? (cancerindex.org)
  • for example, in 1858 Louis Pasteur was the first to show that there is a complex balance of oxygen use in animal cells, and that cells use multiple pathways to accomplish energy con-version (Pasteur, 1858). (nobelprize.org)
  • peptide 14 or 19 from P. gingivalis HSP60 may have either an anti- or pro-atherogenic role, respectively, in the ApoE(−/-) mouse model of infection-triggered atherosclerosis through distinct mechanisms operating in the polarization of T cells. (spandidos-publications.com)
  • Dysfunctional endothelial cells are a hallmark of several cardiovascular diseases, but pathological mechanisms involved are poorly understood. (grantome.com)
  • Aim 2 will investigate the mechanisms by which endothelial-dependent stimuli activate PKD1/PKD2 channels in endothelial cells to produce vasodilation. (grantome.com)
  • Therefore, the present study aimed to elucidate the mechanisms involved in oxLDL‑induced endothelial permeability and monocyte diapedesis under HG conditions, in addition to the potential effects of rosmarinic acid. (spandidos-publications.com)
  • In this study, we aimed to examine the effects of RCE in endothelial cells challenged with hypoxic exposure and to elucidate the underlying mechanisms. (mdpi.com)
  • However, the endothelial cell molecules involved in DENV infection and the mechanisms governing virus-cell interactions are still uncertain. (tmu.edu.tw)
  • 70%. Severe cases exhibit abnormal coagulation, endothelial barrier disruption, and dysfunctional platelet aggregation but the underlying mechanisms remain poorly understood. (cdc.gov)
  • but the molecular mechanisms underlying how cells adapt to variations in oxygen supply were unknown until the prize-winning work described here. (nobelprize.org)
  • P. gingivalis HSP60 at a concentration of 10 ng/l significantly decreased the expression levels of VE‑cadherin and eNOS protein at 24 h stimulation, whereas no difference in these proteins was identified following a low dose of P. gingivalis HSP60 (1 ng/l). (spandidos-publications.com)
  • P. gingivalis HSP60 at 100 ng/l significantly downregulated the expression levels of VE‑cadherin and eNOS protein at 12 h in HUVECs. (spandidos-publications.com)
  • Certain studies show that P. gingivalis lipopolysaccharide (LPS) could induce the expression of intercellular adhesion molecule 1 and vascular cell adhesion molecule 1 in human umbilical vein endothelial cells (HUVECs) ( 13 , 14 ), which significantly enhances trans-endothelial migration of inflammatory cells. (spandidos-publications.com)
  • Binding experiments with radiolabeled monoclonal antibody to P-selectin indicated that there was also surface expression of P-selectin on the AtT-20 cells. (rupress.org)
  • We investigated whether atheroprotective unidirectional pulsatile shear stress affects the expression of PDCD4 in endothelial cells. (plos.org)
  • Methods: Descemet's membranes with corneal endothelial cells (CECs) were obtained during keratoplasty, and expression of aggresomes, type 1 collagen, fibronectin, and agrin was evaluated. (cardiff.ac.uk)
  • ECs were treated with oxLDL under HG conditions in the presence or absence of ROS scavengers mitoTEMPO and NAC, p38 inhibitor SB203580, FOXO1 inhibitor AS1842856 or transfected with the TXNIP siRNA, before protein expression levels of intercellular adhesion molecule 1 (ICAM‑1), vascular cell adhesion molecule‑1 (VCAM‑1), phosphorylated vascular endothelial‑cadherin (VE‑cadhedrin), VE‑cadherin and zonula occludens‑1 (ZO‑1) were measured by western blotting. (spandidos-publications.com)
  • In addition, oxLDL‑stimulated ECs under HG conditions exhibited increased phosphorylated VE‑cadherin protein levels and decreased ZO‑1 protein expression levels compared with those in untreated ECs, suggesting increased endothelial permeability. (spandidos-publications.com)
  • So the objective of our study was to evaluate the effect of inflammation on the expression of proteins in human endothelial cells. (cardiologytalk.com)
  • We then wanted to see what effect EPA, which is an omega-3 fatty acid had on the expression of these protein. (cardiologytalk.com)
  • The protein phosphorylation and expression levels were determined by Western blotting. (medscimonit.com)
  • The expression of bone morphogenic protein 4 (BMP4), a new pro-inflammatory marker, is increased by disturbed flow in endothelial cells (ECs). (houstonmethodist.org)
  • CTXA also inhibited expression and activity of TACE induced by PMA in endothelial cells. (sagepub.com)
  • Proliferating cell nuclear antigen (PCNA) expression in CNV mice without or with YAP siRNA intravitreal injection and the colocalization of PCNA and CD31 were measured with western blotting and immunofluorescent double staining, respectively. (molvis.org)
  • Additionally, after laser exposure, YAP siRNA inhibited the increased expression of PCNA, which was colocalized with endothelial cells. (molvis.org)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • We investigated the homing-related chemokine expression levels of MSCs treated with a prostate cancer cell line (PC-3) -conditioned medium. (cancerindex.org)
  • Expression of IFN-induced transmembrane protein 1 in glomerular endothelial cells. (nih.gov)
  • Expression of stress proteins, adhesion molecules, and. (iifiir.org)
  • Expression of stress proteins, adhesion molecul. (iifiir.org)
  • Expression of stress proteins, adhesion molecules, and interleukin-8 in endothelial cells after preservation and reoxygenation. (iifiir.org)
  • Expression of stress proteins can be considered as a new parameter of preservation-associated endothelial activation. (iifiir.org)
  • Animal cells undergo fundamental shifts in gene expression when there are changes in the oxygen levels around them. (nobelprize.org)
  • These changes in gene expression alter cell metabolism, tissue re-modeling, and even organismal responses such as increases in heart rate and ventilation. (nobelprize.org)
  • Through the combined work of these three laureates it was thus demonstrated that the response by gene expression to changes in oxygen is directly coupled to oxygen levels in the animal cell, allowing immediate cellular responses to occur to oxygenation through the action of the HIF transcription factor. (nobelprize.org)
  • For investigation of RGS5, we hypothesize that silencing RGS5 expression using siRNA technology (5) leads to secretome changes that effect cells at the BBB. (lu.se)
  • Different phenotypes and subtypes of endothelial progenitor cells (EPCs), such as early and late EPCs, have been described according to their functionality. (intechopen.com)
  • These observations strongly suggest that APC might be involved not only in regulation of the coagulation system, but in regulation of inflammatory responses by preventing endothelial cell injury. (eurekaselect.com)
  • Oxidized phospholipids are thought to market atherogenesis by rousing endothelial cells (ECs) to create inflammatory cytokines such as for example IL-8. (careersfromscience.org)
  • Hyperglycemia contributes to glycosylation of proteins and lipids, increases the production of reactive oxygen species, stimulates the synthesis and secretion of pro-inflammatory cytokines and acute phase proteins in various tissues, promoting thus inflammation processes in the vascular wall [ 2 , 3 ]. (medsci.org)
  • Apart from possible protective effects, allograft vasculopathy could be in part a consequence of the antigenic potential of heat shock proteins connected with effects caused by adhesion molecules and inflammatory cytokines. (iifiir.org)
  • Here we pursue an a priori hypothesis that genetic variation in the endothelial-based receptors of the thrombomodulin-protein C system (THBD and PROCR) may similarly be associated with early-onset ischemic stroke. (lu.se)
  • Sphingosine 1-phosphate (Sph-1-P), a bioactive lysophospholipid capable of inducing a wide spectrum of biological responses, acts as an intercellular mediator, through interaction with the endothelial differentiation gene (EDG)/S1P family of G protein-coupled receptors. (nih.gov)
  • It belongs to family 1 of the G protein-coupled receptors. (cancerindex.org)
  • EPCR protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. It belongs to the MHC class I/CD1 family of proteins, that is characterized by having a deep groove, that in other proteins in the family (but not in EPCR) is usually used for antigen binding. (wikipedia.org)
  • The bound lipid in EPCR is usually phosphatidylcholine, but it may be phosphatidylethanolamine, and it contributes to protein C binding, though probably not through direct contact Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy. (wikipedia.org)
  • To investigate whether the mechanism of targeting of P-selectin to granules is specific for megakaryocytes and endothelial cells and/or dependent on von Willebrand factor, a soluble adhesive protein that is stored in the same granules, we have expressed the cDNA for P-selectin in AtT-20 cells. (rupress.org)
  • We hypothesized that macrophage uptake of CBox NPs and secretion of soluble factors mediate enhanced endothelial cell damage abilities of CBox. (cdc.gov)
  • Regulator of G-protein signaling 5 regulates the shift from perivascular to parenchymal pericytes in the chronic phase after stroke. (lu.se)
  • hEPCs have been used for cell-based therapies due to their capacity to contribute in the re-endothelialization of injured blood vessels and neovascularization in ischemic tissues. (intechopen.com)
  • Berger M, Bergers G, Arnold B, Hammerling GJ, and Ganss R. Regulator of G-protein signaling-5 induction in pericytes coincides with active vessel remodeling during neovascularization. (lu.se)
  • APC reduces the pulmonary endothelial cell injury and hypotension in rats administered endotoxin (ET) by inhibiting TNF-α production through inhibition of its transcription. (eurekaselect.com)
  • Patients and Methods: Chart review identified 77 patients with oral or oropharyngeal squamous cell carcinoma treated with gross total surgical resection and postoperative radiation between 1981 and 1992. (johnshopkins.edu)
  • Specifically, the PDCD4 protein combines directly with the mRNA coding region of the target gene ( MYB/c-MYB ) to block translation [2] . (plos.org)
  • What does this gene/protein do? (cancerindex.org)
  • The protein encoded by this gene restricts cellular entry by diverse viral pathogens, such as influenza A virus, Ebola virus and Sars-CoV-2. (nih.gov)
  • The FGD1 gene provides instructions for making a protein that turns on (activates) another protein called Cdc42, which transmits signals that are important for various aspects of development before and after birth. (medlineplus.gov)
  • Variants in the FGD1 gene lead to the production of an abnormally functioning protein. (medlineplus.gov)
  • In males (who have only one X chromosome), one altered copy of the gene in each cell is sufficient to cause the condition. (medlineplus.gov)
  • In autosomal dominant inheritance, one copy of the altered gene in each cell is sufficient to cause the disorder. (medlineplus.gov)
  • In autosomal recessive inheritance, both copies of the gene in each cell have variants. (medlineplus.gov)
  • Hypoxic induction was confirmed using flow cytometric analysis, which showed cell cycle arrest starting at a 200 µM concentration of cobalt chloride. (molvis.org)
  • The formation of new vessels in the hypoxic area restores blood flow, provides oxygen and nutriments to the surviving cells, and promotes the migration and engraftment of new cells. (elifesciences.org)
  • In Sierra Leone during 2015-2018, we assessed LF patients' day-of-admission plasma samples for levels of proteins necessary for coagulation, fibrinolysis, and platelet function. (cdc.gov)
  • The hemostatic system consists of platelets, coagulation factors, and the endothelial cells lining the blood vessels. (medscape.com)
  • Immunofluorescent double staining was used to detect YAP cellular localization with CD31 (an endothelial cell marker) antibody. (molvis.org)
  • Our results indicate that specific regulation of Sph-1-P-modulated migration responses in vascular cells can be achieved by EDG-5 antagonists and that manipulation of Sph-1-P biological activities by each EDG antagonist may lead to a therapeutical application to control vascular diseases. (nih.gov)
  • Using siRNA to knock down PDI, DENV infection was reduced which could be reversed by treating cells with a reducing agent Tris(2-carboxyethyl) phosphine hydrochloride (TCEP). (tmu.edu.tw)
  • We found that both endothelial cell types can phagocytose PMPs, and using TAM-blocking antibodies or siRNA knock-down of individual TAMs we show that the uptake is mediated by endothelial Axl and Gas6. (lu.se)
  • Human brain vascular pericytes treated with either scrambled or RGS5 targeting siRNA, will be co-cultured in a spheroid model of the BBB together with human brain endothelial cells and human astrocytes. (lu.se)
  • The purpose of this study was to investigate the release of Endothelial microparticle (EMP)-associated protein disulfide isomerase (PDI) after endothelial cell injury induced in diabetes and the changes in platelet activation. (aging-us.com)
  • Platelet activation allows binding of these proteins, which bridges adjacent platelets. (medscape.com)
  • The alpha granules contain hemostatic proteins such as fibrinogen, vWf, and growth factors (eg, platelet-derived growth factor and transforming growth factors). (medscape.com)
  • As circulating PMPs-levels were not altered in Gas6-/- mice compared to Gas6+/+ mice, we hypothesize that the Gas6-mediated uptake is not a means to clear the bulk of circulating PMPs but may serve to phagocytose PMPs locally generated at sites of platelet activation and as a way to affect endothelial responses. (lu.se)
  • Since protein disulfide isomerase (PDI) reducing function at the cell surface was shown to be required for entry of certain viruses and bacteria, we explored the role of PDI expressed on endothelial cell surface in DENV infection. (tmu.edu.tw)
  • Using a wide variety of approaches and inducible, endothelial cell-specific knockout mice, we provide evidence that physical coupling of PKD1 to PKD2 in endothelial cells stimulates vasodilation. (grantome.com)
  • Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)
  • Endothelial cell heme oxygenase and ferritin induction by heme proteins: a possible mechanism limiting shock damage. (umn.edu)
  • Dive into the research topics of 'Endothelial cell heme oxygenase and ferritin induction by heme proteins: a possible mechanism limiting shock damage. (umn.edu)
  • In a previous study, it was reported that oxLDL treatment induced nucleotide‑binding domain and leucine‑rich repeat containing family, pyrin domain‑containing 3 inflammasome activation in endothelial cells (ECs) under HG conditions, in a manner that could be effectively reversed by rosmarinic acid. (spandidos-publications.com)
  • The effects of oxLDL‑mediated inflammasome activation on endothelial permeability under HG conditions, in addition to the effects of rosmarinic acid on these oxLDL‑mediated processes, also remain poorly understood. (spandidos-publications.com)
  • Glycocalyx Core Proteins Selectively Mediate Endothelial NOS activation and Cell Alignment in Response to Shear Stress. (ebonglab.org)
  • wp-content/themes/awp-enfold/blank.png 0 0 Academic Web Pages /wp-content/themes/awp-enfold/blank.png Academic Web Pages 2013-01-01 00:00:00 2019-07-09 19:25:47 Glycocalyx Core Proteins Selectively Mediate Endothelial NOS activation and Cell Alignment in Response to Shear Stress. (ebonglab.org)
  • This effective location of protein C may ensure efficacious activation of The common names of Cudrania tricuspidata Bureau protein C on the surface of large vessels. (sagepub.com)
  • They undergo activation, morphological changes and express a protein called Regulator of G-protein Signaling 5 (RGS5) (1, 2). (lu.se)
  • Vascular cell adhesion molecule-1 (VCAM-1), an inducible cell-cell recognition protein on the endothelial cell surface (EC), has been associated with early stages of atherosclerosis. (jci.org)
  • P-selectin (PADGEM, GMP-140, CD62) is a transmembrane protein specific to alpha granules of platelets and Weibel-Palade bodies of endotheial cells. (rupress.org)
  • Endothelial cells line the wall of all blood vessels and regulate a wide variety of functions, including contractility which controls systemic blood pressure. (grantome.com)
  • Endothelial cells form a semi-permeable barrier between the blood and surrounding tissues within all blood vessels in the body. (beckman.com)
  • Endothelial cells regulate the passage of gases, fluid and various molecules across blood vessels by acting as selective filters. (bvsalud.org)