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  • gene
  • Isolated SVAS is caused by genetic changes in the elastin gene (ELN) that can be passed from parent to child. (stlouischildrens.org)
  • In children with Williams syndrome , the elastin gene is deleted along with 25-27 other genes that cause developmental delays and hormone problems. (stlouischildrens.org)
  • The elastin gene provides instructions for making a protein called tropoelastin. (stlouischildrens.org)
  • 1. An isolated nucleic acid encoding an endothelial estrogen regulated gene-7 protein that has (i) an amino acid sequence which has at least about 95% sequence similarity with SEQ ID NO: 2 and (ii) lysyl oxidase activity. (google.com)
  • 2. The isolated nucleic acid of claim 1 , wherein the endothelial estrogen regulated gene-7 protein comprises four scavenger receptor cysteine rich (SRCR) domains having an amino acid sequence at least about 95% identical to the amino acid sequences of SEQ ID NOs: 3, 4, 5, and 6. (google.com)
  • 3. The isolated nucleic acid of claim 2 , wherein the endothelial estrogen regulated gene-7 protein comprises four scavenger receptor cysteine rich domains having the amino acid sequences of SEQ ID NOs: 3, 4, 5, and 6. (google.com)
  • 5. The isolated nucleic acid of claim 1 , wherein the endothelial estrogen regulated gene-7 protein has the amino acid sequence of SEQ ID NO: 2. (google.com)
  • 13. The vector of claim 9 , wherein the endothelial estrogen regulated gene-7 protein is expressed in response to estrogen. (google.com)
  • 18. A method for producing endothelial estrogen regulated gene-7 protein, which method comprises isolating the endothelial estrogen regulated gene-7 protein produced by the host cell of claim 14 , wherein the host cell has been cultured under conditions that provide for expression of the endothelial estrogen regulated gene-7 protein by the vector. (google.com)
  • 19. A method for producing endothelial estrogen regulated gene-7 protein, which method comprises isolating the endothelial estrogen regulated gene-7 protein produced by the host cell of claim 15 , wherein the host cell has been cultured under conditions that provide for expression of the endothelial estrogen regulated gene-7 protein by the vector. (google.com)
  • Therefore, the protein encoded by this gene may play a role in vascular development and remodeling. (nih.gov)
  • interaction
  • The invention further relates to an assay system to identify compounds that selectively modulate EER-7 protein activity by interaction with estrogen. (google.com)
  • Essential for elastic fiber formation, is involved in the assembly of continuous elastin (ELN) polymer and promotes the interaction of microfibrils and ELN (PubMed:18185537). (nih.gov)
  • novel
  • In this work, we constructed novel elastin fusion proteins bearing AG3 silver-binding motifs (GPG-AG3) and their thin films. (ntu.edu.sg)
  • Interestingly, endoplasmic reticulum-targeted elastin-like polypeptide fusions induced the formation of a novel type of protein body, which may be responsible for elastin-like polypeptide's positive effect on recombinant protein accumulation by excluding the heterologous protein from normal physiological turnover. (uwo.ca)
  • Although expressed in the leaves of plants, these novel protein bodies appeared similar in size and morphology to the prolamin-based protein bodies naturally found in plant seeds. (uwo.ca)
  • amino
  • Elastin is restricted to few amino acids and its protein constitutes blood vessels in the body as well as tumors. (edu.gh)
  • It is believed that when the body is deprived of those amino acids that constitute elastin, little amount would be available to constitute those tumors. (edu.gh)
  • Vigna subterranean (A), Cajanus cajan (B), Phaseolus lunatus (C), Canavalia ensiformis (D), Mucuna pruriens (E) were composited to obtain the minimum score of the amino acids that would supply amino acids to build elastin. (edu.gh)
  • Proteins were extracted and hydrolyzed and their amino acids were analyzed with HPLC system. (edu.gh)
  • The amino acids score in elastin (AASE) or values were obtained by simply adding up the six constituent amino acids in elastin (glycine, proline, valine, leucine isoleucine and alanine). (edu.gh)
  • A preponderance of amino acids with small, nonreactive side groups is characteristic for structural proteins, for which H-bonded close packing is more important than chemical specificity. (wikipedia.org)
  • In addition to intra- and intermolecular hydrogen bonds, the distinguishing feature of keratins is the presence of large amounts of the sulfur-containing amino acid cysteine, required for the disulfide bridges that confer additional strength and rigidity by permanent, thermally stable crosslinking-in much the same way that non-protein sulfur bridges stabilize vulcanized rubber. (wikipedia.org)
  • Hydroxyproline is produced by hydroxylation of the amino acid proline by the enzyme prolyl hydroxylase following protein synthesis (as a post-translational modification). (wikipedia.org)
  • Although it is not directly incorporated into proteins, hydroxyproline comprises roughly 4% of all amino acids found in animal tissue, an amount greater than seven other amino acids that are translationally incorporated. (wikipedia.org)
  • Pepsin breaks down the protein in the food into smaller particles, such as peptide fragments and amino acids. (wikipedia.org)
  • The alternative 2.0-kb mRNA encodes a beta-galactosidase-related protein (S-Gal) that is only 546 amino acids long and that has no enzymatic activity. (wikipedia.org)
  • degradation
  • The influence of elastin degradation products, glucose and atorvastatin on metalloproteinase-1, -2, -9 and tissue inhibitor of metalloproteinases-1, -2, -3 expression in human retinal pigment epithelial cells. (semanticscholar.org)
  • Under normoxia (normal oxygen conditions) EGLN1 protein hydroxylates the proline at the 564 position of HIF-1 alpha, which allows ubiquitylation by the von Hippel-Lindau tumor suppressor (pVHL) and subsequent targeting for proteasome degradation. (wikipedia.org)
  • hydroxylation of HIF allows the protein to be targeted for degradation. (wikipedia.org)
  • structural
  • It is thought that these proteins share a structural motif which promotes self-aggregation and fibril formation in proteins through interdigitation of hydrophobic side chains in beta-sheet/beta-turn structures, a motif that has been preserved in recognisable form over several hundred million years of evolution. (wikipedia.org)
  • proline
  • The ability of FKBP65 to modulate the self-assembly of tropoelastin is independent of its enzymatic activity to promote the cis-trans isomerization of proline residues in proteins. (sigmaaldrich.com)
  • heterologous
  • As an alternate approach, yeast expression systems have become an increasingly attractive host for the expression of heterologous proteins 18 , 19 , due to their capacity to be incorporated into industrial-scale fermentation schemes characterized by high cell densities in relatively inexpensive media. (pubmedcentralcanada.ca)
  • In addition, heterologous proteins have been efficiently secreted into the expression medium, resulting in low-cost recovery of the protein. (pubmedcentralcanada.ca)
  • Conclusion: An endoplasmic reticulum-targeted elastin-like polypeptide fusion approach provides an effective strategy for depositing large amounts of concentrated heterologous protein within the limited space of the cell via storage in stable protein bodies. (uwo.ca)
  • complex
  • However, overall protein expression is influenced by two variables, culture cell density and the amount of recombinant protein per cell 20 and as a consequence of the more complex process of protein production in a eukaryotic organism, yields are often low as compared to E. coli expression systems. (pubmedcentralcanada.ca)
  • tropoelastin
  • Elastin binding protein and FKBP65 modulate in vitro self-assembly of human tropoelastin. (sigmaaldrich.com)
  • Both proteins also modify the kinetics of self-assembly of tropoelastin in an in vitro system, consistent with a role in attenuating the premature intracellular self-aggregation of tropoelastin through a mechanism that limits the growth and maturation of aggregates. (sigmaaldrich.com)
  • Elastin is initially present as newly synthesized tropoelastin which can be found in association with S-Gal. (wikipedia.org)
  • enzyme
  • Interestingly, this subunit is identical to the enzyme known as protein disulfide isomerase. (wikipedia.org)
  • Because of this motif-specific behavior, the enzyme also acts on other proteins that contain this same sequence. (wikipedia.org)
  • purification
  • Removal of endotoxin from ELPs has often required one of these secondary purification treatments in addition to traditional purification through temperature induced precipitation, thereby, reducing overall protein yields. (pubmedcentralcanada.ca)
  • nanoparticles
  • Further incubation in silver nitrate yielded silver nanoparticles on the surfaces of the protein particles and thin films. (ntu.edu.sg)
  • The silver nanoparticles obtained in our work had diameters of 20 - 25 nm from protein aggregates solution and 471 nm ± 178 nm on protein films. (ntu.edu.sg)
  • sequence
  • Although not generally homologous to any other protein, soluble lamprins contain a tandemly repeated peptide sequence (GGLGY), which is present in both silk moth chorion proteins and spider dragline silk. (wikipedia.org)
  • Strong homologies to this repeat sequence are also present in several mammalian and avian elastins. (wikipedia.org)
  • eukaryotic
  • Since elastin-like polypeptide is a mammalian-derived protein, this study demonstrates that plant seed-specific factors are not required for the formation of protein bodies in vegetative plant tissues, suggesting that the endoplasmic reticulum possesses an intrinsic ability to form protein body-like accretions in eukaryotic cells when overexpressing particular proteins. (uwo.ca)
  • substrates
  • The GLB1 protein is a beta-galactosidase that cleaves the terminal beta-galactose from ganglioside substrates and other glycoconjugates. (wikipedia.org)
  • genetic
  • Alpha-1 antitrypsin deficiency is a genetic disorder where elastin is excessively degraded by elastase, a degrading protein released by neutrophils during the inflammatory response. (wikipedia.org)
  • functional
  • The elastin-like polypeptide-induced protein bodies were highly mobile organelles, exhibiting various dynamic patterns of movement throughout the cells, which were dependent on intact actin microfilaments and a functional actomyosin motility system. (uwo.ca)
  • endoplasmic reticulum
  • Both the elastin binding protein (EBP) and FKBP65 have been suggested to fulfill that role in the Golgi and endoplasmic reticulum compartments of the cell, respectively. (sigmaaldrich.com)
  • This study's main objectives were to determine the general utility of elastin-like polypeptide protein fusions in various intracellular compartments and to elucidate elastin-like polypeptide's mechanism of action for increasing recombinant protein accumulation in the endoplasmic reticulum of plants. (uwo.ca)
  • Results: The effect of elastin-like polypeptide fusions on the accumulation of green fluorescent protein targeted to the cytoplasm, chloroplasts, apoplast, and endoplasmic reticulum was evaluated. (uwo.ca)
  • The endoplasmic reticulum was the only intracellular compartment in which an elastin-like polypeptide tag was shown to significantly enhance recombinant protein accumulation. (uwo.ca)
  • Intermediate
  • By analysis of the primary structures of these keratins and other intermediate filament proteins, Hanukoglu and Fuchs suggested a model that keratins and intermediate filament proteins contain a central ~310 residue domain with four segments in α-helical conformation that are separated by three short linker segments predicted to be in beta-turn conformation. (wikipedia.org)
  • matrix
  • Recently, yeast and plant expression systems have been explored for the expression of ELPs and related matrix proteins. (pubmedcentralcanada.ca)
  • These reactive aldehydes and allysines can react with lysine and other allysine residues to crosslink and form desmosine, isodesmosine, and a number of other polyfunctional crosslinks that join surrounding elastin molecules to build an elastin matrix and elastic fiber. (wikipedia.org)
  • stable
  • Specifically, hydroxylation increases the melting temperature (Tm) of helical collagen by 16 °C, as compared to unhydroxylated collagen, a difference that allows the protein to be stable at body temperature. (wikipedia.org)
  • digestion
  • Protein digestion, therefore, first starts in the stomach, unlike carbohydrate and lipids, which start their digestion in the mouth. (wikipedia.org)
  • properties
  • Elastin-based biomaterials have gained popularity in tissue engineering and regenerative medicine due to their well-defined chemical and physical properties. (ntu.edu.sg)
  • Yet, elastin-based materials with antimicrobial properties have not been developed to date. (ntu.edu.sg)
  • component
  • This protein component is also released from the surface of cultured chondroblasts by incubation with lactose, and its association with immobilized elastin is inhibited by lactose. (sciencemag.org)
  • gene
  • Isolated SVAS is caused by genetic changes in the elastin gene (ELN) that can be passed from parent to child. (stlouischildrens.org)
  • In children with Williams syndrome , the elastin gene is deleted along with 25-27 other genes that cause developmental delays and hormone problems. (stlouischildrens.org)
  • The elastin gene provides instructions for making a protein called tropoelastin. (stlouischildrens.org)
  • 1. An isolated nucleic acid encoding an endothelial estrogen regulated gene-7 protein that has (i) an amino acid sequence which has at least about 95% sequence similarity with SEQ ID NO: 2 and (ii) lysyl oxidase activity. (google.com)
  • 2. The isolated nucleic acid of claim 1 , wherein the endothelial estrogen regulated gene-7 protein comprises four scavenger receptor cysteine rich (SRCR) domains having an amino acid sequence at least about 95% identical to the amino acid sequences of SEQ ID NOs: 3, 4, 5, and 6. (google.com)
  • 3. The isolated nucleic acid of claim 2 , wherein the endothelial estrogen regulated gene-7 protein comprises four scavenger receptor cysteine rich domains having the amino acid sequences of SEQ ID NOs: 3, 4, 5, and 6. (google.com)
  • 5. The isolated nucleic acid of claim 1 , wherein the endothelial estrogen regulated gene-7 protein has the amino acid sequence of SEQ ID NO: 2. (google.com)
  • 13. The vector of claim 9 , wherein the endothelial estrogen regulated gene-7 protein is expressed in response to estrogen. (google.com)
  • 18. A method for producing endothelial estrogen regulated gene-7 protein, which method comprises isolating the endothelial estrogen regulated gene-7 protein produced by the host cell of claim 14 , wherein the host cell has been cultured under conditions that provide for expression of the endothelial estrogen regulated gene-7 protein by the vector. (google.com)
  • 19. A method for producing endothelial estrogen regulated gene-7 protein, which method comprises isolating the endothelial estrogen regulated gene-7 protein produced by the host cell of claim 15 , wherein the host cell has been cultured under conditions that provide for expression of the endothelial estrogen regulated gene-7 protein by the vector. (google.com)
  • Therefore, the protein encoded by this gene may play a role in vascular development and remodeling. (nih.gov)
  • interaction
  • The invention further relates to an assay system to identify compounds that selectively modulate EER-7 protein activity by interaction with estrogen. (google.com)
  • Essential for elastic fiber formation, is involved in the assembly of continuous elastin (ELN) polymer and promotes the interaction of microfibrils and ELN (PubMed:18185537). (nih.gov)
  • elasticity
  • Skin analysis of elasticity measures concentration of elastin by detecting the degree of polarization of light reflected from the skin using spectral analysis. (thundersplace.org)
  • Collagen is an essential protein that promotes skin elasticity. (reference.com)
  • When the blood contains inadequate amounts of A1AT or functionally defective A1AT (such as in alpha-1 antitrypsin deficiency), neutrophil elastase is excessively free to break down elastin, degrading the elasticity of the lungs, which results in respiratory complications, such as chronic obstructive pulmonary disease, in adults. (wikipedia.org)
  • gene
  • Collagen alpha-2(IV) chain is a protein that in humans is encoded by the COL4A2 gene. (wikipedia.org)
  • Because A1AT is expressed in the liver, certain mutations in the gene encoding the protein can cause misfolding and impaired secretion, which can lead to liver cirrhosis. (wikipedia.org)
  • isolated the lin-4 gene, they found that instead of producing an mRNA encoding a protein, it produced short non-coding RNAs, one of which was a ~22-nucleotide RNA that contained sequences partially complementary to multiple sequences in the 3' UTR of the lin-14 mRNA. (wikipedia.org)
  • Elastin microfibril interfacer 1 (EMILIN-1) is a protein that in humans is encoded by the EMILIN1 gene. (wikipedia.org)
  • A possible polymorphism of the CELA1 gene coding this protein was found. (wikipedia.org)
  • Microfibrillar-associated protein 2 is a protein that in humans is encoded by the MFAP2 gene. (wikipedia.org)
  • DnaJ homolog subfamily C member 30 (DNAJC30), also known as Williams Beuren syndrome chromosome region 18 protein (WBSCR18), is a protein that in humans is encoded by the DNAJC30 gene. (wikipedia.org)
  • This intronless gene encodes a member of the DNAJ molecular chaperone homology domain-containing protein family. (wikipedia.org)
  • Ehlers-Danlos syndrome type 4, caused by mutations of the COL3A gene, leads to defective production of the collagen, type III, alpha 1 protein and causes skin fragility as well as weakness of the walls of arteries and internal organs. (wikipedia.org)
  • Marfan's syndrome results from mutations in the FBN1 gene, defective production of the protein fibrillin-1, and a number of physical abnormalities including aneurysm of the aortic root. (wikipedia.org)
  • tighten
  • The appearance of lines and wrinkles can be partially reversed with the skin care regimen that includes proteins and peptides to hydrate, tighten and tone mature or UV damaged skin and renew surface skin cells. (ulta.com)
  • globules
  • IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. (wikipedia.org)
  • cardiovascular
  • These observations demonstrate that MFAP4, a new player in cardiovascular pathologies, is redundant for normal cardiovascular development and arterial elastin assembly, but plays an important role in regenerative responses of the arterial wall. (mouseclinic.de)
  • While haploinsufficiency of elastin is known to cause the cardiovascular deficiencies, the roles of the other 16 genes in the deleted region, including DNAJC30, have yet to be confirmed. (wikipedia.org)
  • production
  • Other experiments show that a single miRNA species may repress the production of hundreds of proteins, but that this repression often is relatively mild (much less than 2-fold). (wikipedia.org)
  • enzyme
  • The protein was initially named "antitrypsin" because of its ability to bind and irreversibly inactivate the enzyme trypsin in vitro covalently. (wikipedia.org)
  • increases
  • Slight increases in serum bilirubin are best detected by examining the sclerae, which have a particular affinity for bilirubin due to their high elastin content. (wikipedia.org)
  • Functional
  • Mutations in these areas can lead to non-functional proteins that can polymerise and accumulate in the liver (infantile hepatic cirrhosis). (wikipedia.org)
  • secondary structure
  • An all-α proteins is a class of structural domains in which the secondary structure is composed entirely of α-helices, with the possible exception of a few isolated β-sheets on the periphery. (wikipedia.org)
  • α+β proteins are a class of structural domains in which the secondary structure is composed of α-helices and β-strands that occur separately along the backbone. (wikipedia.org)
  • α/β proteins is a class of structural domains in which the secondary structure is composed of alternating α-helices and β-strands along the backbone. (wikipedia.org)
  • Small proteins typically have little secondary structure and often contain multiple disulphide bonds. (wikipedia.org)
  • mutations
  • One study suggested that mutations causing total absence of an MRP6 protein caused a more severe disease, but this could not be confirmed in a subsequent case series. (wikipedia.org)
  • content
  • I thinkBioPeptide EL might be a helper here I am doing research on it now, but after penis surgery things that have its tell tell characteristics belong the capabilities of BioPeptide EL "Lipopeptide" This stuff seems to be able to impact Elastin Content as well as collagen… I'm interested in tipping the scales in favor of greater Elastin content in the TA. (thundersplace.org)
  • nature
  • Scientific evidence shows the complex nature of elastin cannot assimilated at a time proteins our own body to achieve us anything good if taken orally. (soup.io)
  • Numerous protein structures are the result of rational design and do not exist in nature. (wikipedia.org)
  • tissue
  • Disorders of this protein include alpha-1 antitrypsin deficiency, an autosomal codominant hereditary disorder in which a deficiency of alpha-1 antitrypsin leads to a chronic uninhibited tissue breakdown. (wikipedia.org)
  • primarily
  • It forms an underlying aqueous protein-containing hypophase and an overlying phospholipid film composed primarily of dipalmitoyl phosphatidylcholine. (wikipedia.org)
  • main
  • Regular use of Argan oil helps stop the conjoining of proteins and DNA degeneration, the main cause of aging in humans. (argandiva.com)
  • Alpha-1 antitrypsin is the main protein of the alpha-globulin 1 region. (wikipedia.org)
  • Four large classes of protein that are generally agreed upon by the two main structure classification databases (SCOP and CATH). (wikipedia.org)
  • known
  • The C-terminal portion of the protein, known as canstatin, is an inhibitor of angiogenesis and tumor growth. (wikipedia.org)
  • include
  • Common examples include the flavodoxin fold, the TIM barrel and leucine-rich-repeat (LRR) proteins such as ribonuclease inhibitor. (wikipedia.org)
  • hair
  • Internally, the chemistry in hair colour attacks strength-giving proteins in hair and makes the fibre more porous, leading to weaker hair and that fades more quickly. (lebricabracdechristelle.com)
  • surface
  • Reinflation of the alveoli following exhalation is made easier by pulmonary surfactant, which is a phospholipid and protein mixture that reduces surface tension in the thin fluid coating within all alveoli. (wikipedia.org)