Protein antigens cd44. Medical search. Frequent questions

G protein-coupled receptors (GPCRs) are prominent drug targets responsible for extracellular-to-intracellular signal transduction. (bvsalud.org)
Targeting antigens (Ag) to Fcγ receptors (FcγR) intranasally (we. (healthandwellnesssource.org)
Because of this researchers have used novel ways of focus on antigens (Ags) to receptors portrayed on the areas of antigen-presenting cells (APCs) such as for example dendritic cells (DCs) and macrophages in order to RWJ-67657 better activate the mucosal disease fighting capability and elicit solid and protective immune system responses (1). (healthandwellnesssource.org)
Based on the literature and availability of antibody, highly expressed genes (CD24, CD44, and CD74) encoding cell surface receptors and (FABP5 and heat shock protein Hsp27) encoding cytoplasmic proteins were evaluated at protein levels by IHC. (biomedcentral.com)

The following product was used in this experiment: CD44 Monoclonal Antibody (IM7), NovaFluor™ Blue 510, eBioscience™ from Thermo Fisher Scientific, catalog # M010T02B01, RRID AB_2896788. (thermofisher.com)
Description: The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). (thermofisher.com)
Wild-type (WT) and CD44 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD44 antibody [HL1650] (GTX637231) diluted at 1:1000. (genetex.com)
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD44 antibody [HL1650] (GTX637231) diluted at 1:1000 and competitor's antibody diluted at 1:500. (genetex.com)
CD44 antibody [HL1650] detects CD44 protein at cell membrane by immunofluorescent analysis. (genetex.com)
Green: CD44 stained by CD44 antibody [HL1650] (GTX637231) diluted at 1:500. (genetex.com)
The antibody MEM-85 reacts with an extracellular antigen of both cell surface-expressed and soluble form of CD44 antigen (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc. (exbio.cz)
In both H1N1 influenza and SARS-CoV-2 vaccines, rMASE significantly increased antigen-specific antibody secretion, memory T cell engagement, and Th1-biased immune response, which diminished viral loads after lethal challenge. (enlibio.com)
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. (thermofisher.com)
The tissue section was stained with ARG66773 anti-CD44 antibody [SQab20206] at 18°C - 25°C for 30 minutes. (arigobio.cn)

CD44 antigen, the main cell surface receptor for HA. (wikipedia.org)
CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. (thermofisher.com)
We have uncovered a novel functional relationship between the hyaluronan receptor CD44, the matrix metalloproteinase-9 (MMP-9) and the multifunctional cytokine TGF-beta in the control of tumor-associated tissue remodeling. (wdv.com)
CD44 provides a cell surface docking receptor for proteolytically active MMP-9 and we show here that localization of MMP-9 to cell surface is required for its ability to promote tumor invasion and angiogenesis. (wdv.com)
19 normal healthy in- receptor protein (CD117) that is structurally dividuals were recruited as a control group. (who.int)
CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. (exbio.cz)
In this manner, the receptor-binding domain (RBD, surface antigen) of the spike protein was trapped inside the nanocavity, while NP was absorbed on the outside of the droplets, enabling the burst release of NP before RBD. (enlibio.com)
Themis controls thymocyte selection through regulation of T cell antigen receptor-mediated signaling. (ox.ac.uk)
Here we found that Themis had high expression in thymocytes between the pre-T cell antigen receptor (pre-TCR) and positive-selection checkpoints and low expression in mature T cells. (ox.ac.uk)
The inability to predict the foreign antigens that an organism will encounter in its lifetime creates a need for the immune system to generate and maintain a diverse T cell receptor repertoire ( 1 - 3 ). (frontiersin.org)
CD44 is a transmembrane glycoprotein and the major cell surface receptor for the ECM component, hyaluronan. (silverchair.com)
For instance, CD44v6 interacts with c-Met, the receptor of hepatocyte growth factor (HGF), thereby increasing the survival and proliferative ability of tumor cells That is one of the reasons why the expression of the CD44 splice variant CD44v6 is correlated with the metastasis of colon cancer to the liver and a poor clinical prognosis [ 8 ]. (biomedcentral.com)
Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases (PubMed:18757307, PubMed:23589287). (arigobio.cn)
Insulin receptor substrate 1 translocation to the nucleus by the human JC virus T-antigen. (lsuhsc.edu)

CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. (thermofisher.com)
In the case of enveloped RNA viruses, it is the natural dissemination of nucleocapsid protein (NP, core antigen) and surface antigen that delays NP exposure to immune surveillance. (enlibio.com)
By simply reversing the delivery sequence of the surface antigen and core antigen, the inside-out strategy may offer major implications for enhanced vaccinations against the enveloped RNA virus. (enlibio.com)
In the presence of the surface antigen, higher doses of NP resulted in the up-regulated expression of IFN-α, suggesting a robust anti-viral effect (Supplementary Fig. (enlibio.com)
Hyaluronic acid enhances cell proliferation during eosinopoiesis through the CD44 surface antigen. (uchicago.edu)

CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interaction through its affinity for hyaluronic acid (HA) and possibly through other parts of the extracellular matrix (ECM). (antibodyguide.com)

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. (genetex.com)
ERM family members as molecular linkers between the cell surface glycoprotein CD44 and actin-based cytoskeletons. (rupress.org)
We next cloned a cDNA encoding the 140-kD membrane protein and identified it as CD44, a broadly distributed cell surface glycoprotein. (rupress.org)

CD29 complexes with one of nine integrin alpha subunits to form the very late antigen (VLA) subfamily of adhesion molecules. (thermofisher.com)
Also, the flow cytometry analysis showed that ADSCs expressed high levels of stem cell-related antigens (CD13, CD29, CD44, CD105, and CD166), while did not express hematopoiesis-related antigens CD34 and CD45, and human leukocyte antigen HLA-DR was also negative. (thestemcellfoundation.com)

Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. (thermofisher.com)
Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined. (thermofisher.com)
CD44 has numerous isoforms generated through alternative mRNA splicing. (biomedcentral.com)

Two such gene products, CD44 and ezrin, are both upregulated in fibroblasts transformed by v- fos and are commonly implicated in cell motility and invasion. (silverchair.com)
Two such gene products upregulated in these libraries are CD44 and the CD44-interacting protein, Ezrin. (silverchair.com)
The in-depth analysis of gene expression and protein expression in tissue arrays of these candidate genes revealed that they may serve as potential prognostic biomarkers or therapeutic targets for targeted therapy or antigen directed immunotherapy. (biomedcentral.com)

hEGP2, and is a pan-epithelial differentiation antigen that is expressed on almost all carcinomas as 17-1A(mAb) antigen. (proteinkinase.biz)

The link domain is found in some extracellular proteins in vertebrates such as the hyalectans. (wikipedia.org)

The basis of the Genprice Gentaur test is direct agglutination of the killed stained antigen by Brucella canis antibodies. (genomeboy.com)

Among the potential binding partners we identified not only established components of integrin signalling pathways and cytoskeletal proteins, but also several novel LFA-1 binding partners including CD13, galectin-3, thrombospondin-1 and CD44. (northumbria.ac.uk)

CD44 functions as an adhesion molecule through its binding to hyaluronate, an extracellular matrix component. (thermofisher.com)
CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. (exbio.cz)
the majority of these proteins are involved in fibrosis, cell adhesion and extracellular matrix organization. (unina.it)
ITGB1 is a cell adhesion molecule appearing on platelets, as the common Beta subunit of the very late activation antigen (VLA), and as a component of various protein complexes binding to extracellular matrix proteins. (thermofisher.com)
The β2-integrin lymphocyte function-associated antigen 1 (LFA-1) plays an important role in the migration, adhesion and intercellular communication of dendritic cells (DCs). (northumbria.ac.uk)
CD44 is an adhesion molecule for extracellular matrix components such as hyaluronic acid and osteopontin [ 7 ], and plays an important role not only in wound healing and cell migration, but also in tumor invasion and metastasis. (biomedcentral.com)

Immunoprecipitation with various anti-CD44 mAbs showed that ezrin and radixin, as well as moesin, are associated with CD44, not only in BHK cells, but also in mouse L fibroblasts. (rupress.org)
CD44 interacts with ezrin, radixin, and moesin (ERM), linking the actin cytoskeleton to the plasma membrane and the ECM. (antibodyguide.com)
Activation of PKC results in phosphorylation of Ser291, dephosphorylation of Ser325, disassociation of ezrin from CD44, and directional motility. (antibodyguide.com)
Here we report that CD44 and ezrin colocalise to membrane ruffles and microvilli of A431 cells after treatment with EGF. (silverchair.com)
However, A431 cells expressing dominant-negative c-Jun (TAM67), and which as a consequence fail to invade in response to EGF, also fail to correctly localise CD44 and ezrin. (silverchair.com)
CD44 and ezrin are both substrates for Protein Kinase C, and we show that their EGF-dependent colocalisation requires Protein Kinase C activity. (silverchair.com)
Associated with TAM67 expression and disrupted CD44 and ezrin colocalisation is the increased expression and activation of the novel PKC theta isoform. (silverchair.com)
Expression of PKC theta in A431 cells results in the inhibition of cell motility and disrupted localisation of CD44 and ezrin. (silverchair.com)

CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes. (thermofisher.com)
Interestingly, over-expression of extracellular matrices (ECM) related proteins and epithelial-mesenchymal transition (EMT) markers in borderline PTs led us to hypothesize a model of deposition and degradation leading to ECM remodeling and EMT acquisition triggering its malignant transformation. (oncotarget.com)
In addition, expression of limbal epithelial and corneal stromal proteins such as ATP-binding cassette sub-family G member 2 (ABCG2), tumour protein p63 (p63), paired box 6 (Pax6), cytokeratin 3 (AE5), cytokeratin 10, and keratocan sulfate were evaluated either by immunofluorecence staining or reverse transcription polymerase chain reaction. (molvis.org)
Notably, CD44 variants (CD44v) are exclusively expressed in epithelial-type cells, whereas the CD44 standard isoform (CD44s) is expressed in both epithelial and mesenchymal cells [ 9 ]. (biomedcentral.com)

Themis (thymocyte-expressed molecule involved in selection), a member of a family of proteins with unknown functions, is highly conserved among vertebrates. (ox.ac.uk)

HES1-mediated inhibition of Notch1 signaling by a Gemini vitamin D analog leads to decreased CD44(+)/CD24(-/low) tumor-initiating subpopulation in basal-like breast cancer. (nih.gov)
In breast cancer, CD44(+)/CD24(-/low) cells were identified as tumor-initiating cells. (nih.gov)
We previously reported that a Gemini vitamin D analog, 1,25-dihydroxy-20R-21(3-hydroxy-3-deuteromethyl-4,4,4-trideuterobutyl)-23-yne-26,27-hexafluoro-cholecalciferol (BXL0124), reduced CD44(+)/CD24(-/low) cells in MCF10DCIS basal-like breast cancer cells. (nih.gov)
The CD44(+)/CD24(-/low) subpopulation of MCF10DCIS cells showed elevated Notch1 signaling and increased cell proliferation compared to the CD44(+)/CD24(high) subpopulation. (nih.gov)
Five potential novel target genes (FABP5, CD24, CD44, CD74, and HSP27) were identified, which were highly expressed in HNSCC tumor samples and tissue arrays. (biomedcentral.com)
CD24, CD44, and CD74 proteins were expressed on the cell surface, and FABP5 and HSP27 proteins were predominantly expressed in the cytoplasm of HNSCC. (biomedcentral.com)
While additional work is needed to elucidate the biological significance of these proteins, CD24 and CD74 expressed only in small proportion of cells indicating tumor heterogeneity and subtypes of tumor initiating cells (CD24+/CD44+) present in HNSCC. (biomedcentral.com)
CD24 is a 35-45 kD glycosylphosphatidylinositol (GPI)-linked protein also known as heat stable antigen (HSA), BA-1, Ly-52, and nectadrin. (novusbio.com)

High-quality optimized Invitrogen ELISA kits allow you to measure target-specific proteins with confidence, reliability, and consistency. (genomeboy.com)

To identify the integral membrane protein directly associated with ERM family members, we performed immunoprecipitation studies using antimoesin mAb and cultured baby hamster kidney (BHK) cells metabolically labeled with [35S]methionine or surface-labeled with biotin. (rupress.org)
The results indicated that moesin is directly associated with a 140-kD integral membrane protein. (rupress.org)
Using BHK cells as antigens, we obtained a mAb that recognized the 140-kD membrane protein. (rupress.org)
Further comparison to the LFA-1 interaction partners in monocytes indicated that DC differentiation was accompanied by an overall increase in LFA-1 associated proteins, in particular cytoskeletal, signalling and plasma membrane (PM) proteins. (northumbria.ac.uk)
Single-pass type I membrane protein. (arigobio.cn)

A Link domain or Link module, also known as Xlink domain (X for extracellular), is a protein domain that binds to hyaluronic acid. (wikipedia.org)

Immunogen Recombinant protein of human CD44. (antibodyguide.com)

Recombinant protein encompassing a sequence within the center region of human CD44 The exact sequence is proprietary. (genetex.com)
Recombinant Human EpCAM /CD326 Protein, Fc Tag (EPM-H5254) is expressed from human 293 cells (HEK293). (proteinkinase.biz)

CD44 is expressed by hematopoietic and non-hematopoietic cells. (thermofisher.com)
Bone marrow myeloid cells and memory T cells highly express this antigen and peripheral B and T cells can upregulate the expression of CD44. (thermofisher.com)
Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events. (thermofisher.com)
These observations suggest that coordinated CD44, MMP-9, and TGF-beta function may provide a physiological mechanism of tissue remodeling that can be adopted by malignant cells to promote tumor growth and invasion. (wdv.com)
Furthermore, immunofluorescence microscopy revealed that in both BHK and L cells, the Triton X-100-insoluble CD44 is precisely colocalized with ERM family members. (rupress.org)
12. Transcription factor B-cell-specific activator protein (BSAP) is differentially expressed in B cells and in subsets of B-cell lymphomas. (nih.gov)
13. Association of induction of a fully tumorigenic phenotype in murine radiation-induced T-lymphoma cells with loss of differentiation antigens, gain of CD44, and alterations in p53 protein levels. (nih.gov)
dilated myocardium-isolated hmMSC and increased manifestation of cardiac particular proteins during XL413 2 weeks of publicity of cells to SAHA. (exposed-skin-care.net)
Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells. (exbio.cz)
Liu J, Bi G, Wen P, Yang W, Ren X, Tang T, Xie C, Dong W, Jiang G. Down-regulation of CD44 contributes to the differentiation of HL-60 cells induced by ATRA or HMBA. (exbio.cz)
Subramaniam V, Gardner H, Jothy S: Soluble CD44 secretion contributes to the acquisition of aggressive tumor phenotype in human colon cancer cells. (exbio.cz)
Subramaniam V, Vincent IR, Gardner H, Chan E, Dhamko H, Jothy S: CD44 regulates cell migration in human colon cancer cells via Lyn kinase and AKT phosphorylation. (exbio.cz)
CD44 is constitutively phosphorylated at Ser325 in resting cells. (antibodyguide.com)
The cell surface antigens CD44 and CD90 are identified as potential markers for cancer stem cells (CSC) in a number of different cancers, and assumed to be therapeutic targets for cancer cells with stem cell-like properties. (oslomet.no)
A greater percentage of Themis-deficient T cells had CD4(+)CD25(+)Foxp3(+) regulatory and CD62L(lo)CD44(hi) memory phenotypes than did wild-type T cells. (ox.ac.uk)
In wild type mice, Vα3.2 + cells showed higher CD5, CD6 and CD44 expression than non-Vα3-expressing cells, and this was more marked in cells from Themis-deficient mice. (frontiersin.org)
Memory cells, marked by CD44 hi expression, are formed once an infection is cleared, and provide critical protection against re-encounter with the same pathogen. (frontiersin.org)
Apart from antigen induced memory cells, there are also "virtual" or "innate" memory phenotype (MP) cells ( 20 , 21 ), which can also be identified by CD44 hi expression ( 22 ) and which require IL-15 for maintenance ( 23 , 24 ). (frontiersin.org)
However, little is known about the expression of stage-specific embryonic antigen 4 (SSEA-4) and the embryonic stem cell (ESC)-like properties of these cells. (molvis.org)
We studied the expression of SSEA-4 surface protein and the various ESC and MSC markers in the ex vivo cultured limbal stromal cells. (molvis.org)
CD44 has been recently identified as one of the cellular surface markers associated with cancer stem cells (CSCs) in several types of tumors. (biomedcentral.com)
CD44 expression is generally suppressed by p53 binding to the CD44 promoter, so that increased expression of CD44 is detected in tumor cells with mutant p53 [ 10 ]. (biomedcentral.com)
We stimulated CFSE-labeled CD8+ T cells from pmel-1 TCR transgenic mice with the cognate antigen, gp100, in the presence of titrated doses of TWS119 and analyzed them for the expression of the differentiation markers CD44 and CD62L . (pi3kaktresearch.com)

Overflateproteinene CD44 og CD90 er identifisert som potensielle markører for kreftstamceller i flere typer kreft, og antas å være mulige terapeutiske mål for økt spesifikk behandling av kreftceller med stamcellelignende egenskaper. (oslomet.no)
CD44 og CD90 ble i denne studien benyttet som målmarkører for spesifikk behandling med bruk av immuntoksiner (IT). (oslomet.no)
Det ble benyttet fire karsinomcellelinjer og tre liposarkomcellelinjer for å studere effekten av PCI-behandling med IT rettet mot CD44 og CD90. (oslomet.no)
CD44 and CD90 were in this thesis used as targets for a specific treatment with the use of immunotoxins (IT). (oslomet.no)
Four carcinoma cell lines and three liposarcoma cell lines were used as models to study the effect of PCI treatment with IT targeting CD44 and CD90. (oslomet.no)

CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. (thermofisher.com)
We aimed to determine the significance of detecting expression of some antigens that might be pivotal to the process, namely CD44 and CD117, in patients with active pemphigus vulgaris. (who.int)
The immunohistochemical results showed prominent expression of CD44 in 13 of the patients and CD117 in 9 of the patients with new blister formation. (who.int)
Increased surface levels of CD44 are characteristic of T cell activation, and expression of the protein is upregulated during the inflammatory response. (antibodyguide.com)
Surface protein expression was analyzed by flow cytometry to characterize the cell phenotype. (thestemcellfoundation.com)
Loss of p53 results in increased expression of CD44, a cancer stem cell (CSC) marker, which is involved in the scavenging of reactive oxygen species (ROS). (biomedcentral.com)
Here, we report a change in the expression of a CD44 variant isoform (CD44v8-10) in an 8-year-old female LFS patient with osteosarcoma and atypical liver cancer after chemotherapy. (biomedcentral.com)
Detection of human neurotropic JC virus DNA sequences and expression of the viral oncogenic protein in pediatric medulloblastomas. (lsuhsc.edu)
Detection of JC virus DNA sequences and expression of the viral regulatory protein T-Antigen in tumors of the central nervous system. (lsuhsc.edu)
Expression of a polyoma virus oncoprotein and tumor suppressor proteins in medulloblastomas. (lsuhsc.edu)
Expression of JC virus T-Antigen in a multiple sclerosis patient with a Glioblastoma multiforme. (lsuhsc.edu)

We concluded that ERM family members work as molecular linkers between the cytoplasmic domain of CD44 and actin-based cytoskeletons. (rupress.org)

0.05) , augmented nuclear protein-TCF/LEF oligonucleotide interaction and sharply up-regulated Tcf7, Lef1 and other Wnt target genes including Jun, Frizzled7(Fzd7), Nemo-like-kinase (Nlk). (pi3kaktresearch.com)

The AU-rich element RNA-binding protein 1 (AUF1) is an RNA-binding protein, which can both stabilize and destabilize the transcripts of several cancer-related genes. (nature.com)

CD44 concept may raise together with Big t mobile or portable differentiation while CD62L will be progressively lost. (pi3kaktresearch.com)
Remarkably, 46% regarding CD8+ Capital t tissue cultured within the reputation from the greatest power Gsk-3 inhibitor failed to up-regulate CD44, sustaining some sort of "naive" CD44 reduced CD62L large phenotype. (pi3kaktresearch.com)

CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. (thermofisher.com)

Detection of JC polyomavirus DNA sequences and cellular localization of T-Antigen and Agnoprotein in oligodendrogliomas. (lsuhsc.edu)

It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species. (abbkine.com)

Flow cytometry analysis (surface staining) of human peripheral blood with anti-human CD44 (MEM-85) purified / GAM-APC. (exbio.cz)

In addition, we show that MMP-9 localization to the surface of normal keratinocytes is CD44 dependent and can activate latent TGF-beta. (wdv.com)

The well-known function of AUF1 is the destabilization of mRNAs by forming complexes with other proteins. (nature.com)

Anatomy26

Organisms20

Diseases3

Chemicals and Drugs147

Analytical, Diagnostic and Therapeutic Techniques and Equipment30

Phenomena and Processes30

Disciplines and Occupations1

Information Science3

Health Care3

Receptors4

Antibody10

Receptor14

Surface antigen5

Transmembrane glycoprotein1

Glycoprotein3

CD292

Isoforms3

Gene3

Differentiation antigen1

Extracellular proteins1

Antibodies1

CD131

Adhesion6

Ezrin8

Epithelial4

Molecule1

CD248

ELISA1

Membrane5

Hyaluronic1

Immunogen1

Recombinant2

Cells23

CD905

Expression11

Cytoplasmic domain1

0.051

Genes1

CD62L2

Lymphocyte1

Cellular Localization1

Reactivity1

Peripheral blood1

Localization1

Complexes1