AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsInformation ScienceHealth Care
MicrodissectionProstateLaser Capture MicrodissectionProstatic NeoplasmsLasersDissectionReceptors, AndrogenAndrogensProstate-Specific AntigenCell Line, TumorProstatic HyperplasiaGene Expression Regulation, NeoplasticGene Expression ProfilingReverse Transcriptase Polymerase Chain ReactionRNA, MessengerProstatic Intraepithelial NeoplasiaEpithelial CellsImmunohistochemistryTumor Cells, CulturedOligonucleotide Array Sequence AnalysisAdenocarcinomaDihydrotestosteroneHistocytological Preparation TechniquesOrchiectomyProstatectomySperm RetrievalCell ProliferationPolymerase Chain ReactionTumor Markers, BiologicalPygeumApoptosisNeoplasms, Hormone-DependentBlotting, WesternNeoplasm ProteinsParaffin EmbeddingDNA, NeoplasmDisease ProgressionMicromanipulationAndrogen AntagonistsUp-RegulationNeoplasm InvasivenessSignal TransductionGene ExpressionNuclear Receptor CoactivatorsMice, NudeCell DivisionTransurethral Resection of ProstateIntegrin alpha6DoxazosinTissue FixationBase SequenceStromal CellsDown-RegulationMycoplasma hyorhinisCell Transformation, NeoplasticMolecular Sequence DataTransfectionPTEN PhosphohydrolasePromoter Regions, GeneticCell Growth ProcessesTranscription, GeneticTissue EmbeddingCryoultramicrotomyBiopsyLaser TherapyDNA MethylationRNALoss of HeterozygosityHematoxylinCell LineNeoplasm TransplantationAnticarcinogenic AgentsNucleic Acid Amplification TechniquesIsothiocyanatesTransplantation, HeterologousGene Expression RegulationAntineoplastic AgentsIn Situ Hybridization, FluorescenceCell MovementTime FactorsTranscription FactorsAzoospermiaTestosteroneCell CycleRNA, NeoplasmIn Situ HybridizationSensitivity and SpecificityNeoplasm MetastasisGenes, Tumor SuppressorCells, CulturedMice, TransgenicMice, Inbred C57BLProto-Oncogene Proteins c-aktChromosome PaintingProstatic DiseasesDNA-Binding ProteinsFlow CytometryRNA, Small InterferingReproducibility of ResultsCalcitriol
TumorsTissueSpecimensTesticularOvarian CancerBenignMetastaticLaserAndrogen receptorIncreased cell proliferationCDNAProliferationMutationsTissuesProstaticGene expressionPrecursorAmplificationBilateralCancersLung CancerQuantitativeIsolateGenomePopulationsStromalBiopsiesTransurethralBinding proteinTumour cellsGenesProgressionTumorigenesisExpression in prostateRenal cell carcTransiently transfectedBiopsyVitroBreast cancerHeterogeneityProteinsShowed that invMethodsMRNAMicroarrayCancer stemGlioblastomaSpatialTherapeuticInhibitionPatientsMetastasisMolecular and CelCancerous
Tumors15
  • Tumors and complex tissues consist of mixtures of communicating cells that differ significantly in their gene expression status. (biomedcentral.com)
  • RNA-binding proteins can be used as reporters to elucidate components of operational mRNA networks and operons involved in regulating cell-type specific gene expression in tissues and tumors. (biomedcentral.com)
  • Loss of BRCA2 function stimulates prostate malignancy (PCa) cell invasion and is associated with more aggressive and metastatic tumors in PCa individuals. (acancerjourney.info)
  • gene expressions in PCa cells was confirmed using laser capture microdissection in experimental intraosseous tumors and bone metastases of PCa individuals. (acancerjourney.info)
  • manifestation of c-kit using two different intraosseous tumor models and three additional human being PCa cell lines which proved to be c-kit-negative when cultivated or as tumors in non-osseous cells. (acancerjourney.info)
  • Furthermore we demonstrate the contribution of bone-induced c-kit manifestation by PCa cells to intraosseous tumor formation and confirmed high and low expressions of and genes respectively in intraosseous PCa tumors inside a mouse model and bone metastases from PCa individuals. (acancerjourney.info)
  • Before using transduced PCa cells gene induction was verified using cocultures of Rotigotine Computer3 and bone tissue marrow (BM) cells inserted in Matrigel (BD Biosciences) which imitate c-kit induction seen in intraosseous PCa tumors or metastases6. (acancerjourney.info)
  • We have shown recently that in breast and prostate tumors, as well as in breast tumor cell lines, HIF-1α has a crucial role in regulating, either directly or indirectly, the expression of pro-inflammatory genes. (biomedcentral.com)
  • Germline mutations in the VHL gene predispose individuals to a variety of tumors, including renal carcinoma, CNS hemangioblastoma, islet cell tumors of the pancreas, and pheochromocytoma. (nih.gov)
  • Loss of heterozygosity at a locus on chromosome 8 in a high percentage of tumors from patients with prostate carcinoma has been detected and the area of minimal deletion on chromosome 8p12-21 determined. (nih.gov)
  • Using DNA prepared from 99 microdissected tumors, scientists in the UOB analyzed 45 genetic markers spanning a number of chromosomal regions that had been previously implicated in prostate carcinogenesis. (grantome.com)
  • Large hereditary heterogeneity was reported for main tumors from different individuals in research from the nuclear genome, aswell for multiple tumor foci in one individual in research using laser beam microdissection and exome sequencing [17-19]. (irjs.info)
  • Nevertheless, common genetic modifications (and AND Appearance IN Principal TUMORS The (prostate cancers gene 3) cDNA, which is certainly overexpressed in Computer, was discovered by differential screen approximately 15 years back. (irjs.info)
  • Even though these gene expression studies are compromised by the heterogeneity of the tumor, as well as by the difficulty associated with collecting appropriate counterparts to represent normal prostate cells, the gene array data from tumors have shown promising results. (morulaivf.com)
  • It has also emerged as an important modulator of oncogenesis and have been demonstrated to be deregulated in various hematological neoplasms, prostate cancer, thyroid neoplasms, lung cancer, pancreatic and colo-rectal tumors etc 5 . (openaccesspub.org)
Tissue16
  • CTCs are particularly interesting for prostate cancer because metastases occur predominantly bone tissue, which makes biopsies difficult and low yielding. (ubc.ca)
  • Myeloperoxidase (MPO) has been reported in prostate tissue, and considering its pro-oxidant properties, this location might be linked to prostate pathology. (bvsalud.org)
  • In situ hybridization using MPO-specific probes and laser-assisted microdissection for quantitative real-time RT-PCR were performed to observe whether MPO is being produced in prostate tissue. (bvsalud.org)
  • Using the TIVA-tag in conjunction with RNA-seq to investigate transcriptome variance among one dispersed cells and citizen mouse and individual neurons we present which the tissue microenvironment forms the transcriptomic landscaping of specific cells. (ecologicalsgardens.com)
  • Nevertheless many of these strategies depend on sorting private pools of cells in Gossypol suspension system from acutely dissociated tissue in which information regarding cell morphology as well as the microenvironment is Neurog1 normally dropped and where details of one cell variability is normally masked with the averaging impact11. (ecologicalsgardens.com)
  • Here we explain a novel technique for isolating mRNA in morphologically complicated tissue and with the spatial quality of an individual cell utilizing a photoactivatable mRNA catch molecule known as the TIVA-tag. (ecologicalsgardens.com)
  • Outcomes The TIVA-tag catches mobile mRNA upon photoactivation To execute transcriptome evaluation of individually chosen cells Gossypol in unchanged tissue we built a multifunctional photoactivatable mRNA catch molecule that people contact the TIVA-tag. (ecologicalsgardens.com)
  • Dataset from a proteomics analysis of tumor antigens shared between an allogenic tumor cell lysate vaccine and pancreatic tumor tissue. (maartenmemorial.com)
  • Using a novel technique to microdissect tissue under direct microscopic visualization, pure populations of prostate carcinoma have been procured from prostate specimens. (nih.gov)
  • In order to investigate the genetic events associated with the initiation and progression of prostate cancer, scientists from the Urologic Oncology Branch (UOB) and the Laboratory of Pathology (LOP) at the NCI have made use of a number of innovative methodologies centered around the procurement of highly purified prostatic cancer cells from heterogeneous tissue. (grantome.com)
  • The known locations can be targeted, for cells with certain histological characteristics, or untargeted, on a grid over the tissue. (github.io)
  • Laser microdissection predates ISH, though it was not used for spatial transcriptomics until it was possible to profile the transcriptome from small quantity of tissue. (github.io)
  • We investigated if an adequate histological diagnosis can be made from tissue after Thulium laser vapo-enucleation of the prostate (ThuVEP) and whether it is comparable to transurethral prostate resection (TURP) tissue findings in patients with symptomatic benign prostatic hyperplasia. (serenamaruccia.it)
  • Tissue/cell type and diagnosis for each specimen on TMA is confirmed by a pathologist. (bcm.edu)
  • While a grade describes the appearance of cancer cells and tissue, a cancers stage explains how large the primary tumor is and how far the cancer has spread in the patients body. (healthyskinworld.com)
  • The determination of the spatial arrangement of molecules in cells and tissue samples is the basis for biological research and clinical practice and has gained the attention of several entities involved in the field of genetics. (grandviewresearch.com)
Specimens2
  • To better understand the gene expression patterns in tumor-associated stroma, laser-capture-microdissections from clinical specimens were analyzed by genome-wide-expression microarray technology. (morulaivf.com)
  • Sequencing of a nucleic acid of single cells or specific biological specimens has increased, which has driven the need for automated, robust, and scalable library preparation solutions. (grandviewresearch.com)
Testicular4
Ovarian Cancer3
  • Expression of Osteoblast - Specific Factor 2 (OSF- 2 , Periostin) Is Associated with Drug Resistance in Ovarian Cancer Cell Lines. (morulaivf.com)
  • One of the main obstacles to the effective treatment of ovarian cancer patients continues to be the drug resistance of cancer cells. (morulaivf.com)
  • The obtained results indicate that OSF-2 expression might be associated with the development of DOX and MTX resistance in the primary serous W1 ovarian cancer cell line. (morulaivf.com)
Benign6
Metastatic5
  • Tumour heterogeneity describes the observation that different tumour cells can show distinct morphological and phenotypic profiles, including cellular morphology, gene expression, metabolism, motility, proliferation, and metastatic potential. (wikipedia.org)
  • This dissertation presents a new workflow to isolate, extract, and sequence single CTCs from patients with metastatic prostate cancer. (ubc.ca)
  • This workflow was used to successfully enumerate CTCs from 20 patients with metastatic castrate resistant prostate cancer, as well as 25 patients with localized prostate cancer. (ubc.ca)
  • Using this process, we enriched and isolated 30 single CTCs from 3 patients with metastatic prostate cancer, and sequenced 5 of these single CTCs from a patient with matched cell-free DNA. (ubc.ca)
  • METHODS: PD-L1 expression was measured using five antibody clones (22C3, 28-8, CAL10, E1L3N and SP142) and the therapeutic antibody atezolizumab on 222 lung, 71 ovarian, 52 prostate and 267 breast cancers, and 54 metastatic lesions. (ru.nl)
Laser15
  • We then developed a process to isolate single CTCs using laser capture microdissection for genome sequencing. (ubc.ca)
  • Glandular epithelial cells and stromal areas were separately isolated PF 4708671 using laser-capture microdissection (LCM). (healthweeks.com)
  • Single-cell laser microdissection was utilized to identify the minimal number of tumor cells needed for mutation detection. (takarabio.com)
  • Other strategies such as laser beam catch microdissection (LCM) and patch pipette aspiration (PPA)12 13 can isolate one cells in tissues but both these strategies have restrictions including potential RNA contaminants from various other cells that are in incidental connection with the patch pipette. (ecologicalsgardens.com)
  • the most recent such technique involving laser capture of cells greatly increased the speed and purity of dissection. (grantome.com)
  • UC researchers analyzed the AMACR gene's abnormal expression patterns using a sophisticated laser-capture microdissection technique to identify the key biological events that lead to colon cancer progression. (sciencedaily.com)
  • LCM, also known as laser microdissection (LMD), is by far the most commonly used method of microdissection. (github.io)
  • UV LCM is also known as laser microbeam microdissection (LMM) due to the microbeam of UV laser used. (github.io)
  • Pure tumor epithelia were isolated using laser capture microdissection from 602 samples. (ru.nl)
  • desk S3) was also verified by quantitative RT-PCR (qPCR) and/or IHC analyses (Fig. 6C-F). Significantly, qPCR evaluation of RNA ready from epithelial glands isolated by laser beam catch microdissection (LCM) verified the epithelial-specific distinctions in gene appearance amounts between 6-month-old wt and transgenic VPs (Fig. 6D). (cancer-pictures.org)
  • Are Histological Findings of Thulium Laser Vapo-Enucleation Versus Transurethral Resection of the Prostate Comparable? (serenamaruccia.it)
  • Thulium Laser Prostate Enucleation in Refractory Urinary Retention: Operative and Functional Outcomes in a Large Cohort of Patients. (serenamaruccia.it)
  • To evaluate the functional and operative results of thulium laser enucleation of the prostate (thulep) in patients with indwelling catheters for refractory urinary retention. (serenamaruccia.it)
  • Various articles have previously addressed the introduction of new surgical laser therapies for an enlarged prostate gland causing obstructive symptoms. (serenamaruccia.it)
  • Laser capture microdissection with the Arcturus XT instrument is a way for researchers to enrich a sample for a particular cell type or group of cells. (bcm.edu)
Androgen receptor1
Increased cell proliferation2
CDNA1
  • cDNA libraries have been developed from normal, low-grade PIN, high-grade PIN, and prostate cancer. (nih.gov)
Proliferation8
  • Cell Proliferation Migration Invasion and Anoikis Cell viability and proliferation of PCa cells stably transfected using the vector expressing c-kit or the unfilled vector were examined utilizing the WST-1 assay within the existence or lack of 100 ng/ml SCF (PeproTech). (acancerjourney.info)
  • Appearance of 15-LOX2sv-b, a splice variant that does not have AA-metabolizing activity, leads to a moderate epithelial hyperplasia but obvious prostate enlargement connected with elevated cell proliferation and luminal cells just (Fig. 7). (cancer-pictures.org)
  • These recognizable adjustments in gene appearance bring about two mobile final results, i.e., prominent hyperplasia (with upsurge in amounts of both luminal and basal cells) connected with improved proliferation due to 13(S)-HODE and early induction of senescence induced by 15(S)- HETE, which might cancel one another out leading to minor prostate enhancement. (cancer-pictures.org)
  • Failing to degrade hnRNPL marketed ovarian cancers cell proliferation VU0652835 in tumor and vitro development vivo, phenocopying the scarcity of FBXO16 in ovarian cancers. (azd1152.com)
  • SKOV3 cells steady expressing control, hnRNPL WT or hnRNPL RRM3 had been analyzed for BrdU cell VU0652835 proliferation. (azd1152.com)
  • Indeed, FBXO16 has a critical function in the legislation from the proliferation, clonogenic success, and cell invasion capability of ovarian cancers cells in a way reliant on its E3 ligase activity. (azd1152.com)
  • In FBXO16-lacking ovarian cancers cells, depleting hnRNPL not merely inhibited cell proliferation, clonogenic success, and cell invasion, but repressed the activation of signaling pathways such as for example RAS also, EMT, and MAPK, that have been linked to the molecular mechanism and natural function of FBXO16 carefully. (azd1152.com)
  • More importantly, steady expressing a hnRNPLRRM3 mutant that can't be regarded and degraded by FBXO16 phenocopied FBXO16 insufficiency in ovarian cancers cells, indicating FBXO16 stimulates the invasion and proliferation of ovarian cancers cells mainly via hnRNPL degradation. (azd1152.com)
Mutations6
  • A minimal level of intra-tumour heterogeneity is a simple consequence of the imperfection of DNA replication: whenever a cell (normal or cancerous) divides, a few mutations are acquired-leading to a diverse population of cancer cells. (wikipedia.org)
  • Stem cell variability is often caused by epigenetic changes, but can also result from clonal evolution of the CSC population where advantageous genetic mutations can accumulate in CSCs and their progeny (see below). (wikipedia.org)
  • In this model, tumours arise from a single mutated cell, accumulating additional mutations as it progresses. (wikipedia.org)
  • Evolution of the initial tumour cell may occur by two methods: Sequentially ordered mutations accumulate in driver genes, tumour suppressor genes, and DNA repair enzymes, resulting in clonal expansion of tumour cells. (wikipedia.org)
  • These results demonstrate the potential of our single cell sequencing workflow to discover clinically relevant mutations from single CTCs that may aid in monitoring disease progression and guiding treatment. (ubc.ca)
  • This study is the first to explain how a gene-AMACR-is regulated in relation to cancer development and to identify specific genetic events (a polymorphism and somatic cell mutations) related to colon cancer. (sciencedaily.com)
Tissues8
  • catch from one cells in live tissues. (ecologicalsgardens.com)
  • However the research of one mammalian cells in tissues can help in deciphering the resources of one cell variability and specifically the way the microenvironment establishes variability in cells of apparently the same type. (ecologicalsgardens.com)
  • Notably the tissues microenvironment made by specific neighboring cells could be significantly diverse which is anticipated that with extracellular heterogeneity comes gene appearance heterogeneity. (ecologicalsgardens.com)
  • Therefore equipment that check out the transcriptome from one cells in tissues would give a unique chance of evaluating mammalian cell heterogeneity and its own natural importance. (ecologicalsgardens.com)
  • This is also true for cells in complicated tissues like the brain where in fact the mobile connective intricacy of intermingling neurons and glia makes one cell mRNA isolation difficult. (ecologicalsgardens.com)
  • Therefore an mRNA catch methodology that's appropriate for live intact tissues and that allows Gossypol mRNA catch with precise spatial quality would give a useful device to explore the transcriptomes of one cells in the framework of their organic microenvironment with small bias from RNA contaminants or experimentally-related damage. (ecologicalsgardens.com)
  • We demonstrate the tool from the TIVA-tag in both cell lifestyle and brain Gossypol tissues for catch of one cell mRNA for following RNA-seq transcriptome evaluation. (ecologicalsgardens.com)
  • The minuscule amount of transcripts from microdissected tissues, which can be single cells, needs to be amplified to be detected by microarray or RNA-seq. (github.io)
Prostatic7
Gene expression10
  • In order to understand how different cell types influence one another's gene expression, it will be necessary to monitor the mRNA profiles of each cell type independently and to dissect the mechanisms that regulate their gene expression outcomes. (biomedcentral.com)
  • However, the gene expression profile of a whole tumor corresponds to the combined profiles of the different cell types contained within it (e.g. endothelial cells, T-cells, cancer cells, stromal cells, etc. (biomedcentral.com)
  • Monitoring the dynamics of gene expression in each cell type of a tumor during angiogenesis will advance understanding of tumorigenesis as well as organogenesis, in general. (biomedcentral.com)
  • Our lab has devised biochemical and immunological approaches to gene expression profiling by using RNA-binding proteins as reporters of discrete mRNA subsets in metazoan cells [ 8 - 10 ]. (biomedcentral.com)
  • While we and other labs have demonstrated the isolation of mRNA subsets that are potentially co-regulated using RNA binding proteins as reporters of gene expression, methods have not been described that provide information about coordinated posttranscriptional regulation within specific types of cells during tumorigenesis and development. (biomedcentral.com)
  • Therefore, it will be necessary to assess changes in gene expression that occur when cells such as tumor cells and endothelial cells interact in order to understand growth control and critical processes such as angiogenesis. (biomedcentral.com)
  • Using this approach, we were able to determine how the gene expression profiles of endothelial cells change in response to the presence of breast cancer cells. (biomedcentral.com)
  • Adaptation to hypoxia and consequent pro-inflammatory gene expression of prostate and breast carcinomas have been implicated in the progression toward cancer malignant phenotype. (biomedcentral.com)
  • GBM stem and differentiated cells were incubated under hypoxic conditions and analyzed for pro-inflammatory gene expression and for invasive/migratory behavior. (biomedcentral.com)
  • In collaboration with scientists in the Laboratory of Pathology , a technique for recovering and analyzing RNA from microdissected prostate cancer and by differential gene expression analysis has been developed, and a novel zinc finger gene upregulated in prostate cancer has been identified. (nih.gov)
Precursor1
  • The identical genetic loss has been shown to be associated with prostate intraepithelial neoplasia (PIN), a precursor lesion in prostate cancer. (nih.gov)
Amplification5
  • Here are the latest publications comparing PicoPLEX technology to other single-cell whole-genome amplification technologies, and using PicoPLEX technology to uncover insights from CTCs, single cells from FFPE tumor samples, and samples relevant to preimplantation genetic testing. (takarabio.com)
  • Comparative study of whole genome amplification and next generation sequencing performance of single cancer cells. (takarabio.com)
  • This paper compared the performance of three commercially available whole genome amplification (WGA) technologies: the PicoPLEX WGA Kit , the REPLI-g Single Cell Kit, and the Ampli1 WGA Kit. (takarabio.com)
  • Performance of four modern whole genome amplification methods for copy number variant detection in single cells. (takarabio.com)
  • This paper compared the performance of copy number variation (CNV) detection of four commercially available whole genome amplification (WGA) kits: the PicoPLEX WGA Kit , the GenomePlex Single Cell Whole Genome Amplification Kit, the MALBAC Single Cell Whole Genome Amplification Kit, and the REPLI-g Single Cell Kit. (takarabio.com)
Bilateral1
  • Affected individuals with this hereditary form of renal cell carcinoma are at risk of developing multifocal, bilateral hereditary papillary renal cell carcinoma. (nih.gov)
Cancers1
  • As FBXO16 can modulate and correlate numerous mobile signaling VU0652835 pathways in ovarian cancers cells adversely, we. (azd1152.com)
Lung Cancer1
  • This talk will focus on Manchester's experience of circulating tumour cells and cell-free DNA in lung cancer. (uib.no)
Quantitative2
  • Semi-quantitative evaluation of mRNA amounts was performed by real-time PCR using SYBR Green and gene appearance profile of c-kit-transfected and control PCa cells was analyzed using a gene-focused array (find Supporting Information Components and Strategies). (acancerjourney.info)
  • We obtained peripheral blood mononuclear cells (PBMCs) from 100 patients with MDD and 53 healthy controls and used quantitative reverse transcription-polymerase chain reaction analysis for negative regulators of TLR 4 signaling studies. (jneuropsychiatry.org)
Isolate1
  • Here we demonstrate that RNA-binding proteins can be used to isolate mRNA populations representing total cell mRNA from specific types of cells, as well as discrete mRNA subpopulations that represent post-transcriptionally regulated subsets of mRNAs that encode functionally related proteins. (biomedcentral.com)
Genome3
  • Single and pooled tumor cells (SK-BR-3) obtained from EDTA- and CellSave-preserved blood and archival material were whole-genome amplified. (takarabio.com)
  • This paper compared the performance of the PicoPLEX DNA-seq Kit , the DOPlify WGA Kit, the REPLI-g Single Cell Kit, and the Ampli1 WGA Kit for aneuploidy screening and copy number analysis using shallow whole genome sequencing, starting from one, three, or five cells isolated from the Loucy cell line. (takarabio.com)
  • Cells were whole-genome amplified, followed by PCR-free library preparation and Illumina sequencing. (takarabio.com)
Populations2
  • Existing methods have got been successful at isolating mRNA from populations of living cells including neurons using manual sorting stream cytometry or immunopanning8-10. (ecologicalsgardens.com)
  • Advantages of ICM over flow cytometry include low set up cost, smaller number of nuclei required and greater sensitivity for non-diploid cell populations. (nature.com)
Stromal1
  • The epithelial-stromal interaction plays a critical role in prostate development, reactive changes, and tumorigenesis. (morulaivf.com)
Biopsies2
Transurethral1
  • The group was successfully able to show the feasibility and safety of magnetic resonance imaging (MRI) - controlled transurethral ultrasound therapy for prostate cancer in eight patients. (pharmaceuticalintelligence.com)
Binding protein2
  • Here we demonstrate that the epitope-tagged RNA binding protein, PABP, expressed separately in tumor cells and endothelial cells can be used to discriminate their respective mRNA targets from mixtures of these cells without significant mRNA reassortment or exchange. (biomedcentral.com)
  • In this study, we define a model system for using poly (A) binding protein (PABP) to recover mRNAs from specific cell-types in mixed cell cultures. (biomedcentral.com)
Tumour cells5
  • There are two models used to explain the heterogeneity of tumour cells. (wikipedia.org)
  • The cancer stem cell model asserts that within a population of tumour cells, there is only a small subset of cells that are tumourigenic (able to form tumours). (wikipedia.org)
  • The CSC model posits that the heterogeneity observed between tumour cells is the result of differences in the stem cells from which they originated. (wikipedia.org)
  • The tumor microenvironment may also contribute to tumour expansion, as it is capable of altering the selective pressures that the tumour cells are exposed to. (wikipedia.org)
  • Multiple types of heterogeneity have been observed between tumour cells, stemming from both genetic and non-genetic variability. (wikipedia.org)
Genes6
  • Moreover, using this approach we identify a set of endothelial genes that respond to the presence of co-cultured breast tumor cells. (biomedcentral.com)
  • Hypoxic stem cell lines showed a clear time-dependent activation of the entire panel of pro-inflammatory genes as compared to differentiated tumor cells. (biomedcentral.com)
  • We have also evidenced a similar pattern of overexpressed genes in GBM-SCs after hypoxic treatment, showing also a gain of invasive and migratory function that was lost when these stem cells differentiated. (biomedcentral.com)
  • Chromosomal deletions are often found in the vicinity of genes that protect cells from becoming cancerous. (grantome.com)
  • Three jnk genes are expressed in eukaryotes and their products were initially thought to convey cell death signals. (utexas.edu)
  • We discovered that in FBXO16-lacking cells also, the appearance from the downstream genes of EMT and RAS pathways more than doubled, followed by improved MAPK activity notably. (azd1152.com)
Progression2
  • Since CTCs are accessible from peripheral blood, these cells represent a potential source of highly relevant tumor materials, which could be used to reveal new biomarkers for monitoring disease progression and evaluating drug efficacy. (ubc.ca)
  • From this research, several gene interaction networks inferred could provide clues for the mechanism of prostate cancer progression. (biomedcentral.com)
Tumorigenesis1
  • We have found that in response to activation of RTKs, JNKs play critical roles in tumorigenesis, DNA damage, cell migration, and metastasis. (utexas.edu)
Expression in prostate2
Renal cell carc1
  • A new hereditary cancer syndrome, hereditary papillary renal cell carcinoma (HPRC), has been described. (nih.gov)
Transiently transfected2
  • To discard nonspecific results on gene appearance cells had been transiently transfected using the pcDNA3 unfilled vector (Invitrogen). (acancerjourney.info)
  • The VHL protein is found both in the nucleus and in the cytosol of transiently transfected cells. (nih.gov)
Biopsy1
  • Research greater than 3,000 sufferers showed the fact that urine-based check for expression helps not merely in your choice for do it again prostate biopsy in sufferers with raised PSA amounts but also in the principal PC medical diagnosis [32]. (irjs.info)
Vitro2
  • However, in vitro experiments showed the ability of MPO to potentiate the ROS production and inflammation on prostate epithelial cells. (bvsalud.org)
  • During his NCI tenure, Dr. Ahmed managed a portfolio of grants in signal transduction, radiation immune response, cancer stem cells, and in vitro and in vivo models in radiotherapy. (tcgcrest.org)
Breast cancer2
  • Evidence of the cancer stem cell model has been demonstrated in multiple tumour types including leukemias, glioblastoma, breast cancer, and prostate cancer. (wikipedia.org)
  • Gooch, J.L., Van Den Berg, C.L., Yee, D. Insulin-like growth factor (IGF)-I rescues breast cancer cells from chemotherapy-induced cell death- proliferative and anti-apoptotic effects. (utexas.edu)
Heterogeneity3
  • The heterogeneity of cancer cells introduces significant challenges in designing effective treatment strategies. (wikipedia.org)
  • A key challenge in CTC isolation and characterization has been their extreme rarity in blood and their cell-to-cell heterogeneity. (ubc.ca)
  • Nevertheless cells of apparently the same type aren't identical on the molecular level1-3 and demonstrate a differing amount of heterogeneity amongst their portrayed mRNAs and proteins which may be influenced by mobile stimulation. (ecologicalsgardens.com)
Proteins3
  • For example, we identified subpopulations of mRNAs that are associated with ELAV/Hu RNA-binding proteins that are expressed in specific cell types [ 10 ]. (biomedcentral.com)
  • Moreover, because many different mRNA-binding proteins in specific cell types are known to interact with unique subpopulations of mRNAs encoding functionally related proteins [ 9 - 15 ] they can be informative of the dynamic effects of cells on one another. (biomedcentral.com)
  • Launch Multicellular microorganisms are comprised of an array of cells that are grouped into different kinds based on phenotypic traits such as for example area morphology lineage origins and proteins or RNA biomarker profile. (ecologicalsgardens.com)
Showed that inv1
  • Biological assays showed that invasive and migratory behavior was strengthened by hypoxia only in GBM stem cells. (biomedcentral.com)
Methods3
  • Methods have been devised to generate mRNA samples from specific types of tumor cells. (biomedcentral.com)
  • Transcriptome profiling can be an indispensable tool in advancing the knowledge of one cell biology but is dependent upon methods with the capacity of Gossypol isolating mRNA on the spatial quality of an individual cell. (ecologicalsgardens.com)
  • High adoption compared to spatial genomic methods, for the transcriptomic study of single cells is a key segment driver. (grandviewresearch.com)
MRNA3
  • The TIVA technique provides the initial noninvasive strategy for recording mRNA from one cells within their organic microenvironment. (ecologicalsgardens.com)
  • Nevertheless RNA-seq of one cells is bound by the specialized challenges connected with isolating mRNA from one cells. (ecologicalsgardens.com)
  • Increased expression of OSF-2 mRNA was observed in drug-resistant cells and followed by increased protein expression in cell culture media of drug-resistant cell lines. (morulaivf.com)
Microarray1
  • Diverse microarray technologies have been used to characterize the molecular changes in prostate cancer. (morulaivf.com)
Cancer stem1
  • These are the cancer stem cell model and the clonal evolution model. (wikipedia.org)
Glioblastoma1
  • The aim of our study was to analyze the hypoxic and pro-inflammatory microenvironment in GBMs and to demonstrate that in a stem/progenitor cell line derived from human glioblastoma (GBM-SCs), hypoxia activates a coordinated inflammatory response, evidencing an invasive and migratory phenotype. (biomedcentral.com)
Spatial1
  • Spatial genomics and transcriptomics offer unique advantages in studying the spatial organization and interactions of cells within complex biological systems. (grandviewresearch.com)
Therapeutic1
  • In a few research, appearance in the urine sediment attained after prostate therapeutic massage was examined by real-time PCR with TaqMan probes. (irjs.info)
Inhibition1
  • Inhibition of bone-induced manifestation in PCa cells transduced with lentiviral short hairpin RNA reduced intraosseous tumor incidence and growth. (acancerjourney.info)
Patients1
  • Initially, we investigated the morphology of CTCs from patients with prostate cancer, and observed that CTCs and leukocytes were similar in size, but distinct in nucleus-to-cytoplasm ratio, which suggests the potential to separate CTCs based on deformability. (ubc.ca)
Metastasis1
  • However despite such obvious evidence suggesting the induction of c-kit manifestation is triggered by the osseous microenvironment Rotigotine as a result of an Rotigotine adaptation of the PCa bone colonizing cells the contribution of c-kit manifestation and activation in PCa cells to bone metastasis has not been confirmed up to date. (acancerjourney.info)
Molecular and Cel1
  • 11 Department of Molecular and Cell Biology, Verna and Marrs McLean Department of Biochemistry, Alkek Center for Molecular Discovery, Baylor College of Medicine, Houston, Texas. (aacrjournals.org)
Cancerous1
  • This method has been applied to two well known prostate cancer data sets: hormone sensitive versus hormone resistant, and healthy versus cancerous. (biomedcentral.com)