Properties embryonic stem cells. Medical search. Frequent questions

Embryonic stem cells are derived from early embryos and have the ability to differentiate into any cell type. (spiked-online.com)
They are made from cells found in very early human embryos, called blastocysts. (eurostemcell.org)
Human embryos reach the blastocyst stage 4-5 days post fertilization, at which time they consist of 50-150 cells. (wikipedia.org)
Isolating the inner cell mass (embryoblast) using immunosurgery results in destruction of the blastocyst, a process which raises ethical issues, including whether or not embryos at the pre-implantation stage have the same moral considerations as embryos in the post-implantation stage of development. (wikipedia.org)
Embryonic stem cells (ESCs), derived from the blastocyst stage of early mammalian embryos, are distinguished by their ability to differentiate into any embryonic cell type and by their ability to self-renew. (wikipedia.org)
For instance, Science University and Oregon Health are engaged in developing blastocyst staged embryos of around 150 cells in order to provide hESC source. (grandviewresearch.com)
The issue of research involving stem cells derived from human embryos is increasingly the subject of a national debate and dinner table discussions," said President George W. Bush in a 2001 speech announcing his policy on embryonic stem cell research. (erlc.com)
Where do the embryos for embryonic stem cells come from? (erlc.com)
Currently, all human embryonic stem cell lines in use today were created from embryos generated by IVF. (erlc.com)
One aspect to this project will be to source oocytes, or immature egg cells to generate SCNT embryos from which embryonic stem cells are harvested. (medicalxpress.com)
President Bush, saying he wanted to "proceed with great care," announced in a national address on August 9 that he would allow federal funding of an existing 60 stem-cell lines but would not permit tax dollars to pay for the destruction of any additional human embryos. (christianitytoday.com)
The rule circumvented a 1995 congressional ban on using federal money for biomedical research on embryos outside the womb by allowing researchers to use stem cells extracted by a third party. (christianitytoday.com)
The groups argue that rather than waste embryos that will be destroyed along with their stem cells, researchers should use them to help save those whose lives are being cut short by disease. (christianitytoday.com)
Indeed, some observers believe the demand for stem cells is dangerously close to spawning a huge commercial industry around the sale of and experimentation on human embryos. (christianitytoday.com)
Already, news that Advanced Cell Technology-a Massachusetts-based, privately held biotech company-and Virginia Medical School's Jones Institute had created or planned to create human embryos for the sole purpose of extracting their stem cells has troubled those on both sides of the debate. (christianitytoday.com)
They are little leftover balls of 50 to 100 cells or so called a blastocyst, excess embryos created in fertility clinics. (metrotimes.com)
The ethical and political controversy dates back to 1998, when human embryonic stem cells were first isolated from donated human embryos. (empowher.com)
This ES cell line does not require feeder cells for propagation and readily transmits through the germline using outbred CD1 4-8 cell embryos as hosts. (seattlechildrens.org)
After becoming chief pathologist at the University of Wisconsin-Madison Wisconsin Regional Primate Center in 1995, James A. Thomson began his pioneering work in deriving embryonic stem cells from isolated embryos. (asu.edu)
Stem cells may be derived from adult tissues but the most potent are extracted from developing human embryos. (edu.au)

and 7,029,913 - are all entitled "Primate embryonic stem cells," and pertain to specific purified preparations of primate ESCs, as well as methods for isolating the cells. (consumerwatchdog.org)
Human embryonic stem cells (ESCs) are pluripotent cells, meaning cells that can make any other cell in the body. (eurostemcell.org)
Many areas of science use mouse ESCs to study how blastocysts grow into adults and to study what signals direct stem cells to differentiate into specialised cells. (eurostemcell.org)
An immense amount has been learned about embryonic development and disease from creating 'chimera' mice with genetically modified ESCs. (eurostemcell.org)
Researchers are learning how to create many different specialised cell types found in the body by exposing ESCs to different signalling molecules and growth conditions. (eurostemcell.org)
Mouse ESCs are being used to learn about how the body develops, from early embryonic stages to the formation of complex organs. (eurostemcell.org)
Another challenging area is controlling exactly how ESCs differentiate into numerous specialised cell types. (eurostemcell.org)
Embryonic stem cells (ESCs) are pluripotent stem cells derived from the inner cell mass of a blastocyst, an early-stage pre-implantation embryo. (wikipedia.org)
When provided with the appropriate signals, ESCs initially form precursor cells that in subsequently differentiate into the desired cell types. (wikipedia.org)
ESCs divide very frequently due to a shortened G1 phase in their cell cycle. (wikipedia.org)
Retinoblastoma proteins that inhibit the transcription factor E2F until the cell is ready to enter S phase are hyperphosphorylated and inactivated in ESCs, leading to continual expression of proliferation genes. (wikipedia.org)
Despite this difference in the cell cycle when compared to ESCs grown in media containing serum these cells have similar pluripotent characteristics. (wikipedia.org)
Embryonic stem cells (ESCs) are stem cells that have been taken from the inner cell mass of a blastocyst, an embryo of about 150 cells that has not yet implanted into a woman's uterus. (erlc.com)
The first mammalian embryonic stem cells (ESCs) were derived from mice and have proven very useful for studying gene function and the impact of changes to individual genes. (scienceblog.com)
In addition, human ESCs proliferate much more slowly than do cells derived from mice and grow in flat, two-dimensional colonies, while mouse ESCs form tight, three-dimensional colonies. (scienceblog.com)
It is been extremely difficult to propagate human ESCs from a single cell, which prevents the creation of genetically manipulated human embryonic stem cell lines. (scienceblog.com)
They derived human induced pluripotent stem cells (iPSCs) - which are created by reprogramming adult cells and have many of the characteristics of human ECSs, including resistance to manipulation - in cultures containing the growth factor LIF, which is used in the creation of mouse ESCs. (scienceblog.com)
The resulting cells visibly resembled mouse ESCs and proved amenable to a standard gene manipulation technique that exchanges matching sequences of DNA, allowing the targeted deactivation or correction of a specific gene. (scienceblog.com)
These progenitors which are derived from either embryonic stem cells (ESCs) or healthy induced pluripotent stem cells (iPSCs) express wild-type levels of a-syn, thus making them equally susceptible to developing Lewy bodies over time. (lu.se)

If an inner cell mass is taken from a mouse blastocyst and given the right nutrients, the pluripotent cells can grow in the laboratory. (eurostemcell.org)
Embryonic stem cells of the inner cell mass are pluripotent, meaning they are able to differentiate to generate primitive ectoderm, which ultimately differentiates during gastrulation into all derivatives of the three primary germ layers: ectoderm, endoderm, and mesoderm. (wikipedia.org)
Pluripotent stem cells have shown promise in treating a number of varying conditions, including but not limited to: spinal cord injuries, age related macular degeneration, diabetes, neurodegenerative disorders (such as Parkinson's disease), AIDS, etc. (wikipedia.org)
Here, we extended the approach to describe the genome topologies of differentiated human lymphoid and myeloid cells derived from human induced pluripotent stem cells. (ca.gov)
Specifically, we have generated differentiated human myeloid and DP thymocytes from induced pluripotent stem cells (iPS). (ca.gov)
We also would aim to describe in detail the 3D-architecture of lymphoid and myeloid genomes that are derived from human induced pluripotent stem cells. (ca.gov)
These are the pluripotent cells that possess the ability to get differentiated into all germ layers. (grandviewresearch.com)
They can be derived directly from isolated organs or differentiated from embryonic stem cells or induced pluripotent stem cells. (springeropen.com)
Human liver organoid (HLO) derived from human pluripotent stem cells offers. (springeropen.com)
Human pluripotent stem cells (hPSCs)-derived brain organ. (springeropen.com)
The evolution of pluripotent stem cell-derived retinal organoids (ROs) has brought remarkable opportunities for developmental studies while also presenting new therapeutic avenues for retinal diseases. (springeropen.com)
Scientists from the Monash Institute of Medical Research (MIMR) and colleagues from New South Wales will compare two different methods of creating patient-specific stem cells: somatic cell nuclear transfer (SCNT) and induced pluripotent stem cells (iPS). (medicalxpress.com)
Researchers from the Massachusetts General Hospital Center for Regenerative Medicine (MGH-CRM) and the Harvard Stem Cell Institute have a developed a new type of human pluripotent stem cell that can be manipulated more readily than currently available stem cells. (scienceblog.com)
Since the growth factors appeared to make such a difference, the researchers tried to make a more useful human pluripotent cell using a new approach. (scienceblog.com)
Organotypic Cocultures of Human Pluripotent Stem Cell Derived-Neurons with Mammalian Inner Ear Hair Cells and Cochlear Nucleus Slices. (bionicsinstitute.org)
Here we use metabolic labelling with stable isotopes and mass spectrometry to quantitatively address the global turnover of genomic methylcytidine (mdC), hydroxymethylcytidine (hmdC) and formylcytidine (fdC) across mouse pluripotent cell states. (uni-muenchen.de)
In primed pluripotent cells the global mdC turnover rate is about 3-6% faster than can be explained by passive dilution through DNA synthesis. (uni-muenchen.de)
Thus, in pluripotent cells active mdC turnover involves both mdC oxidation-dependent and independent processes. (uni-muenchen.de)
We studied human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) measuring the SNPs and CNVs with Affymetrix SNP 6 microarrays and expression values with Affymetrix Exon microarrays. (biomedcentral.com)
Our results revealed that in the human pluripotent stem cells, the expression values of several genes, transcripts and exons were affected due to the genomic variation. (biomedcentral.com)
Derivation of Pluripotent Stem Cells from Cultured Human Primordial Germ Cells' (1998), by John Gearhart et al. (asu.edu)
In November 1998, two independent reports were published concerning the first isolation of pluripotent human stem cells, one of which was "Derivation of Pluripotent Stem Cells from Cultured Human Primordial Germ Cells. (asu.edu)
Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells' (2007), by Junying Yu et al. (asu.edu)
On 2 December 2007, Science published a report on creating human induced pluripotent stem (iPS) cells from human somatic cells: "Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. (asu.edu)
Adult stem cells are classified as pluripotent and are undifferentiated cells that remain quiescent in tissues until stimulated, when they can create cell types that are compatible with the tissue in which they reside. (bvsalud.org)
Direct neuronal reprogramming of a somatic cell into therapeutic neurons, without a transient pluripotent state, provides new promise for the large number of individuals afflicted by neurodegenerative diseases or brain injury. (lu.se)
For gene therapy, a combinatorial approach with multiple target genes, as well as more complex optogenetic approaches are studied, while for cell-therapy human induced pluripotent stem cells and human induced neuronal cells are investigated. (lu.se)
Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)
Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)
However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
These strat- egies provide a robust way to visualize and isolate specific cell pop- ulations of differentiated pluripotent stem cells. (lu.se)

We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)

My research interests focus mainly on stem cells (embryonic, 'adult' and fetal origins) and their applications in tissue engineering, particularly the osteoblast and bone tissue. (nottingham.ac.uk)
however, whether they can mimic the properties of fetal tenocytes is unknown. (cam.ac.uk)
Furthermore, this study adds to the growing evidence that monolayer cultured cells' gene expression profiles converge, with adult and fetal tenocytes having only 10 significantly different genes when cultured in this manner. (cam.ac.uk)
Conclusion: The information provided in this study makes a significant contribution to the investigation into the differences between adult reparative and fetal regenerative cells and supports the concept of using ESC-derived tenocytes as a cellular therapy. (cam.ac.uk)
Transplantations of fetal tissue in the 1980s and 1990s provided proof-of-concept for the potential of cell replacement therapy for PD and some patients benefitted greatly from their transplants. (lu.se)
NSCs are derived from the embryonic stem (ES) cells or fetal and adult nervous system, and can generate different type of neural cells. (lu.se)
The prenatal period is further subdivided into 2 periods, the embryonic (see the following image) and fetal periods. (medscape.com)
Graph showing the embryonic period (first 6 wk of life) in relation to the fetal period. (medscape.com)

Due to their plasticity and potentially unlimited capacity for self-renewal, embryonic stem cell therapies have been proposed for regenerative medicine and tissue replacement after injury or disease. (wikipedia.org)
In addition to their potential in regenerative medicine, embryonic stem cells provide a possible alternative source of tissue/organs which serves as a possible solution to the donor shortage dilemma. (wikipedia.org)
The differentiated cells are further used for therapeutic applications in regenerative medicine. (grandviewresearch.com)
If stem cells can be used to produce new and differentiated cells that are damaged because of disease (such as Parkinson's disease) or injury (e.g., spinal cord damage), it would transform regenerative medicine. (erlc.com)
MSCs played a crucial role in regenerative therapy and have been introduced as an interdisciplinary field between cell biology and material science. (techscience.com)
They may also serve as an unlimited source of therapeutically active cells for regenerative medi- cine. (lu.se)
UniStem Day is the largest educational outreach initiative on stem cells and regenerative medicine in Europe. (lu.se)

Pluripotency distinguishes embryonic stem cells from adult stem cells, which are multipotent and can only produce a limited number of cell types. (wikipedia.org)
What are adult stem cells? (erlc.com)
The term adult stem cells simply refers to any non-embryonic stem cell, whether taken from a fetus, a child or an adult. (erlc.com)
Adult stem cells are sometimes referred to as somatic stem cells to differentiate them from human germ cells, sperm cells and egg cells. (erlc.com)
Currently adult stem cells are being used to treat more than 75 different diseases. (thefamuanonline.com)
Adult stem cells have other properties that make them more attractive to private firms than their embryonic counterparts. (thefamuanonline.com)
When taken from a patient's own body, adult stem cells are not rejected. (thefamuanonline.com)
On the other hand, only three labs have shown that adult stem cells can indeed be transformed into other cells that make up a human body. (thefamuanonline.com)
They felt this proved they were right, that it was good enough to only use adult stem cells! (metrotimes.com)
Adult stem cells exist in adult tissues throughout the body, such as bone marrow, brain, muscle and GI tract. (empowher.com)
Many studies have shown the utility of embryonic or adult stem cells for forming teeth and for regeneration of bone and soft tissues. (bvsalud.org)
By definition, adult stem cells are capable of differentiation into at least two lineages and have the property of self-renewal. (bvsalud.org)
Clinical research had demonstrated the therapeutic benefits of interventions using adult stem cells rather than embryonic cells, a direction that guaranteed respect for human dignity, even at the embryonic stage. (who.int)

First, WARF created a policy allowing companies without a license to the cells to sponsor research on hESCs at academic or non-profit institutions. (consumerwatchdog.org)
This investigation aimed to characterize the biophysical and molecular properties of exosomes harvested from human embryonic stem cells (hESCs) following the guidelines of the International Society for Extracellular Vesicles. (ucalgary.ca)

Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
However, it can be challenging to differentiate when they are connected with wounded tissue, opposed to when they instruct tissue-specific progenitor cells responsible for the redevelopment of damaged tissue. (techscience.com)
Recent studies have isolated distinct cell populations from infantile hemangiomas that display properties equivalent to aberrant progenitor cells, suggesting that, in addition to malignant tumors, benign tumors may also contain a stem cell-like component. (medscape.com)
The first part of the thesis (Paper I, II, III) shows the development and improvement of a hESC-based system of for virus-mediated direct reprogramming of human glial progenitor cells into both induced dopaminergic neurons (iDANs) and GABAergic interneurons. (lu.se)

Pluripotency factors Oct4 and Nanog play a role in transcriptionally regulating the embryonic stem cell cycle. (wikipedia.org)
The developmental progression of embryonic stem cells into specialist cell types involves the activation of lineage-specific programs of gene expression and the silencing of genes involved in maintaining pluripotency. (ca.gov)
This talk addresses aspects of the behaviour of polymers under confinement: the first concerns a novel mechanical property of the mouse embryonic stem cell nucleus exiting pluripotency, the property of auxeticity, studied using a combined biophysical and dynamical systems approach. (imsc.res.in)
This process accelerates upon acquisition of primed pluripotency and returns to low levels in lineage committed cells. (uni-muenchen.de)
While more and more long intergenic non-coding RNAs (lincRNAs) were identified to take important roles in both maintaining pluripotency and regulating differentiation, how these lincRNAs may define and drive cell fate decisions on a global scale are still mostly elusive. (biomedcentral.com)
These findings provide supporting evidence that enrichment for proteins involved in pluripotency is not restricted solely to malignant tumors as is suggested by the "stem cell theory of cancer", but additionally extends to common benign vascular tumors such as hemangiomas. (medscape.com)

A renewable, tissue culture source of human cells capable of differentiating into a wide variety of cell types would have broad applications in basic research and therapeutic techniques. (spiked-online.com)
Researchers are currently focusing heavily on the therapeutic potential of embryonic stem cells, with clinical use being the goal for many laboratories. (wikipedia.org)
In this regard, cell-based therapy may be an alternative therapeutic approach. (lu.se)
SCNT, or therapeutic cloning, is one method used to produce a source of individually-tailored stem cells. (medicalxpress.com)
Cultured human embryonic stem cell (hESC) lines are an invaluable resource because they provide a uniform and stable genetic system for functional analyses and therapeutic applications. (nih.gov)

Mesenchymal stem cells (MSCs) are multipotent cells usually isolated from bone marrow, endometrium, adipose tissues, skin, and dental pulp. (techscience.com)
Mesenchymal stem cells (MSCs) are multipotent stromal cells capable of self-renewal and exhibit multilineage differentiation properties. (techscience.com)

Noninvasive detection and imaging of molecular markers in live cardiomyocytes derived from human embryonic stem cells Biophysical Journal. (nottingham.ac.uk)
the EST, which assesses the effects of compounds on the differentiation of mouse embryonic stem cells into contracting cardiomyocytes, can be used to rank the potency of chemicals within a series of alkoxyacetic acid metabolites formed from The implementation of the European REACH (Registration, glycol ethers. (cdc.gov)

The report notes that the AAR, which bills itself as the leading citizen advocacy organization for improving the health of older Americans, "also happens to receive funding from private-sector biotechnology companies that have a financial stake in the outcome of the stem-cell debate, including Geron," the for-profit corporation that isolated embryonic stem cells in 1998. (christianitytoday.com)

Loring, who is an adjunct associate professor of stem cells and regeneration at Burnham, told BTW that the patents should not exist because there was substantial prior art. (consumerwatchdog.org)
Second, stem cells may prove to be an indispensable source of transplantable cells and tissues for repair and regeneration. (erlc.com)
A thematic series in Cell Regeneration . (springeropen.com)
The Editor-in-Chief of Cell Regeneration will review all submissions prior to peer review and may reject any of them that do not fit the scope of the journal or do not meet the journal's standards for peer review. (springeropen.com)
Stem cells are regarded as the ideal resource for tissue regeneration, outside of formal organ transplantation. (empowher.com)
The autologous graft is still considered the gold standard, as it has the necessary properties for bone regeneration, in terms of combined osteoconduction, osteoinduction and osteogenesis [ 5 5. (scielo.br)
Stem cells are nonspecific cells with powerful self-regeneration properties and they are capable of organizing other cell types in the body. (bvsalud.org)

However, the mechanism underlying the regulation of transcriptional bursting kinetics (burst size and frequency) in mammalian cells remains elusive. (janelia.org)
These results uncovered the key molecular mechanisms underlying transcriptional bursting and cell-to-cell gene expression noise in mammalian cells. (janelia.org)
Our results lend support to the concept of post-transcriptional RNA operons, but we further present evidence that nuclear proximity of genes may provide substantial functional regulation in mammalian single cells. (nih.gov)

The key to these cells' utility is their ability to differentiate into many different cell types depending on the stimulus received and they have been used in treatments for diseases such as cancer and neural degeneration, in rehabilitation of tetraplegic and paraplegic patients and even in dentistry 1 . (bvsalud.org)

Starting from a depolarized (proliferative) cell, the organism grows to a certain size, limited by the increasingly polarized state after successive proliferation events. (nature.com)
The system reaches homeostasis, with a depolarized core (proliferative cells) surrounded by a rim of polarized cells (non-proliferative in this condition). (nature.com)
Carcinogenesis is introduced through a localized event (a spot of depolarized cells) or by random depolarization of cells in the tissue, which returns cells to their initial proliferative state. (nature.com)

At this time I also began working with mouse and human embryonic stem cells resulting in the publication of some of the first papers describing the osteogenic differentiation of mouse ES and human cells in vitro and in vivo . (nottingham.ac.uk)

Blastocytes obtained through nuclear transfers would be used to generate the embryonic stem cells that could be differentiated to specific tissues or organs for transfer to the nuclear donor. (spiked-online.com)
These germ layers generate each of the more than 220 cell types in the adult human body. (wikipedia.org)
Particularly, human embryonic stem cells (hES cells), which can give rise to all tissues of an organism (Figure A), are used to generate mature cells of the heart (Figure B) allowing us to study radiation/microgravity-induced cardiovascular alterations, which may occur during long-term space missions. (gsi.de)
Human embryonic stem (hES) cells can generate cells expressing p63, K14, and involucrin, which have been proposed to be keratinocytes. (harvard.edu)
As stem cells within a developing human embryo differentiate within the cell, their capacity to diversify generally becomes more limited and their ability to generate many differentiated cell types also becomes more restricted. (erlc.com)
Genetic changes introduced into hLR5-iPSCs would be retained when they are coverted back to iPSCs, which we then can use to generate cell lines for future research, drug development and someday stem-cell based gene-correction therapies," says Geijsen. (scienceblog.com)

Scientists were able to produce from human embryonic stem cells a highly pure, large quantity of functioning neurons that will allow them to create models of and study diseases such as Alzheimer's, Parkinson's, prefrontal dementia and schizophrenia. (sciencedaily.com)
Researchers previously had been able to produce neurons - the impulse-conducting cells in the brain and spinal cord - from human embryonic stem cells. (sciencedaily.com)
However, the percentage of neurons in the cell culture was not high and the neurons were difficult to isolate from the other cells. (sciencedaily.com)
UCLA's Yi Sun, an associate professor of psychiatry and biobehavioral sciences, and Howard Hughes Medical Institute investigator Thomas Südhof at the University of Texas Southwestern Medical Center were able to produce 70 to 80 percent of neurons in cell culture. (sciencedaily.com)
We're excited because we have been able to purify so many more neurons out of the cell culture and they were, surprisingly, healthy enough to form synapses. (sciencedaily.com)
Sun's method prodded human embryonic stem cells to differentiate into neural stem cells, the cells that give rise to neurons. (sciencedaily.com)
When the time was right, Sun's team added protein growth factors into the cell culture that stopped the neural stem cells from self-renewing and prodded them into differentiating into neurons. (sciencedaily.com)
The neurons could then be separated from the neural stem cells that had not yet differentiated, a sort of chemical round-up that isolated the neurons. (sciencedaily.com)
The cells were then put into a cell strainer that allowed passage through of the isolated neurons. (sciencedaily.com)
We will be able to study the cellular properties of neurons in a very defined way that will maybe tell us what goes wrong in diseases such as Alzheimer's and Parkinson's," Sun said. (sciencedaily.com)
Using the same techniques to prod the two embryonic stem cells lines to differentiate, Sun found that one line had a bias to become neurons that are found in the forebrain. (sciencedaily.com)
If you're studying a disease found in a certain part of the brain, you should use a human embryonic stem cell line that produces the neurons from that region of the brain to get the most accurate results from your study. (sciencedaily.com)
Huntington's disease, for example, is a forebrain disease, so the neurons should be differentiated from a cell line that is biased to produce neurons from the forebrain. (sciencedaily.com)
Sun said there are ways to prod an embryonic stem cell line biased to become neurons found in the rear brain to become neurons found in the forebrain. (sciencedaily.com)
Sun and her team confirmed that the two embryonic stem cell lines were different through gene expression analysis -- neurons that perform different functions in different parts of the brain express different genes. (sciencedaily.com)
After 35 days in vitro (DIV), hdINs showed electrophysiological properties and spontaneous synaptic currents comparable to mature neurons. (lu.se)
Because of this local degeneration of a relatively small population of dopaminergic neurons in the midbrain, PD has been considered an especially interesting candidate for cell-replacement therapy. (lu.se)
First, we utilized single cell sequencing to dissect the differentiation of stem cells to midbrain dopaminergic neurons. (lu.se)
This approach could be potentially applied directly in the brain by targeting resident cells as a source of new neurons. (lu.se)
Prof. Lindvall and Dr. Kokaia were the first in the world to show that the brain produces new neurons in response to stroke which opened new possibilities for cell therapy by developing the way to modulate and direct brains this self-repair mechanisms. (lu.se)
We provide detailed protocols for the generation of lentiviral vectors, the transduction of hES-cells, and their differentia- tion into neurons. (lu.se)

Researchers are learning how cells assemble into complex tissues, such as the layers of the brain, by attempting to grow primitive organs in the lab. (eurostemcell.org)
Stem cells derived through such techniques can be converted into liver, nerve, and heart tissues. (grandviewresearch.com)
Cells Tissues Organs (2011) 193 (4): 239-252. (karger.com)
Additionally, they can form specialized cell types from other tissues if they are transplanted 6 . (bvsalud.org)
Stromal cells are a mixed population that can create bone, cartilage and fat and also fibrous and connective tissues. (bvsalud.org)

Current screening of potential new drugs is done using cell lines derived from animals or 'abnormal' human tissue such as tumor cells. (spiked-online.com)
The exact process of differentiation is not yet understood and although embryonic stem cells can, in principle, provide for all human tissue, scientists are some way from controlling the process. (spiked-online.com)
According to a 2002 article in PNAS, "Human embryonic stem cells have the potential to differentiate into various cell types, and, thus, may be useful as a source of cells for transplantation or tissue engineering. (wikipedia.org)
In tissue engineering, the use of stem cells are known to be of importance. (wikipedia.org)
Controlled differentiation that can be achieved in tissue committed cells through the co-culturing with basic fibroblast growth factor is expected to induce growth. (grandviewresearch.com)
For this purpose, we use human stem cells that play a pivotal role in tissue development, maintenance and function. (gsi.de)
Organoids are cultured cellular structures that consist of the major cell types of a tissue including tissue stem cells and other differentiated cells, resemble the basic features and possess the functions of the tissue. (springeropen.com)
I returned full time to Imperial College in 1999 to take up a lectureship in cell biology and tissue engineering and was also actively involved in establishing the Tissue Engineering Centre. (nottingham.ac.uk)
I was also affiliated with the Wolfson Centre for Stem Cells, Tissue Engineering and Modelling (STEM), part of the Centre for Biomolecular Sciences, which is now the Biodiscovery Institute. (nottingham.ac.uk)
Researchers value the cells for their ability to replicate quickly and turn into any kind of human tissue. (christianitytoday.com)
Enhanced expression of IL-6, IGF-1, TGF-β, and VEGF has also been observed in LC treated BMSCc+ groups, suggested the cardiac differentiation of BMSCc+, and can be utilized in tissue engineering for cardiac cell therapy. (techscience.com)
The Mesenchymal and Tissue Stem Cell Committee, which belongs to the International Society for Cellular Therapy, stated that plastic-adherent properties are the minimum criteria for determining MSCs. (techscience.com)
In the present model, the bioelectric context of cells and tissue is the field responsible for organization, as it regulates cell proliferation and the level of communication driving the system's evolution. (nature.com)
This simplified model of embryogenesis, tissue organization and carcinogenesis, based on non-excitable cells' bioelectric properties, can be made more realistic with the introduction of other components, like biochemical fields and mechanical interactions, which are fundamental for a more faithful representation of reality. (nature.com)
Upper arm muscle biopsies from female patients are taken, and the muscle cells and connective tissue cells are grown in culture and then injected into the urethra of the same women. (empowher.com)
Several genome-wide studies have reported genomic variants that correlate with gene expression levels in various tissue and cell types. (biomedcentral.com)
These cells are classified as totipotent and they can form any of the tissue types found in the adult body, in addition to having unlimited proliferation potential 6 . (bvsalud.org)
However, post-mortem analysis of transplanted tissue revealed accumulation of pathological Lewy bodies in a small subset of transplanted cells over time, revealing a host-to-graft disease propagation. (lu.se)
Dr CHAUHAN (India)1 welcomed the updated WHO Guiding Principles on Human Cell, Tissue and Organ Transplantation. (who.int)

Scientists anticipate that in the future stem cell lines will provide a virtually unending supply of pancreatic cells for diabetic patients, neuronal cells for patients with neural disorders such as Parkinson's or Alzheimer's disease, and a host of heart cells that may treat a variety of cardiac problems. (spiked-online.com)
Direct neuronal conversion of resident glial cells is advantageous since they are ubiquitously distributed brain cells able to self-renew and replenish their number, making them ideal candidates for endogenous repair. (lu.se)

Electrophysiological properties of neurosensory progenitors derived from human embryonic stem cells. (bionicsinstitute.org)
Next, in order to study the potential of autologous cell replacement therapy we transplanted progenitors derived from a PD patient into a pre-clinical rat model. (lu.se)

The cells are being studied to be used as clinical therapies, models of genetic disorders, and cellular/DNA repair. (wikipedia.org)
Significant involvement of the Japanese government in the development of cell line based therapies is a significant factor and is expected to provide lucrative growth avenues. (grandviewresearch.com)
hES cells also serve as a basis for cerebral organoid models (Figure C) to better understand e.g. the mechanisms of cognitive impairment and radiation necrosis as severe side effects of radiation therapies. (gsi.de)
The petition recognizes that many "Canadians suffer from debilitating illnesses and diseases" and that the petitioners "support ethical stem cell research that has already shown encouraging potential to provide cures and therapies for these illnesses and diseases. (lifesitenews.com)
Biotechnology companies specializing in stem-cell research stand to reap huge financial windfalls from successful therapies developed via this science," said the CPI report. (christianitytoday.com)
The data presented in this thesis may serve as valuable resources to help optimize future cell replacement therapies for patients suffering from PD. (lu.se)

Recent developments in spectroscopic techniques allow highly sensitive image detection both in vitro and in vivo on the individual cell level. (nanowerk.com)
Accelerated uptake of ESC based cell therapy on in vivo and in vitro principle is expected to fuel growth through to 2024. (grandviewresearch.com)
We portrayed the Notch system in embryonic stem cell (ESC)-derived embryoid bodies (EBs) differentiating under the standard protocols used to assess yolk sac (YS) hematopoiesis in vitro. (karger.com)
In vitro toxicity data of these metabolites derived in the development of validated and accepted in vitro and in silico embryonic stem cell test were used as input in the PBK model to extrapolate in vitro concentration-response curves to predicted approaches is urgently needed. (cdc.gov)
To determine how the pathogenesis of these viruses differs, we compared their ability to induce disease in mice and replicate and induce cell death in vitro. (cdc.gov)

Here, the stem cell line is created using the genetic properties of the prospective recipient via somatic cell nuclear transfer. (spiked-online.com)
체세포 핵 치환 (Somatic-cell nuclear transfer, SCNT)은 난자 의 핵 을 제거한 후에, 체세포 의 핵을 이식하여 복제 를 하는 기술을 말한다. (wikipedia.org)

Not only might stem cell technology obviate the need for cadaver donation - it could also resolve the problem of rejection. (spiked-online.com)
In contrast, embryonic stem cell lines can have surface proteins, which cause rejection. (thefamuanonline.com)

Cell metabolism plays vital roles in organismal development, but it has been much less studied than transcriptional and epigenetic control of developmental programs. (springeropen.com)
Comparing two- and three-dimensional culture also indicates three-dimensional culture as being a more physiologically relevant culture system for determining transcriptomic difference between the same cell types from different developmental stages. (cam.ac.uk)

Scientists use genetically altered mice made from ES cells to study genes involved in many human diseases. (eurostemcell.org)
The immense value of this technique for science and medicine was recognized in 2007 when the Nobel Prize in Medicine was awarded to three scientists who pioneered the use of ES cells to create genetically modified mice . (eurostemcell.org)
However, he added, 'we really don't know enough yet about the properties of these genetically altered cells, nor if they stay stable over long periods of time. (metrotimes.com)

Transcriptional bursting is the stochastic activation and inactivation of promoters, contributing to cell-to-cell heterogeneity in gene expression. (janelia.org)
Alternatively, transgenesis and gene targeting techniques can be used to introduce the patient's genes into the stem cell line. (spiked-online.com)
These findings were important since they provide new insights as to how such regulatory elements find each other with great speed, selectivity and specificity to induce lineage specific patterns of gene expression both in embryonic stem cells and differentiated progeny. (ca.gov)
These cells will be excellent for doing gene expression studies and biochemical and protein analyses. (sciencedaily.com)
Single-cell RNA sequencing studies on gene co-expression patterns could yield important regulatory and functional insights, but have so far been limited by the confounding effects of differentiation and cell cycle. (nih.gov)
We apply a tailored experimental design that eliminates these confounders, and report thousands of intrinsically covarying gene pairs in mouse embryonic stem cells. (nih.gov)
These covariations form a network with biological properties, outlining known and novel gene interactions. (nih.gov)
As described in the June 4 Cell Stem Cell, these new cells could be used to create better cellular models of disease processes and eventually may permit repair of disease-associated gene mutations. (scienceblog.com)
Functional studies on GPR81, the gene harboring one of the 10 CpGs, indicated its distinct impacts on TMZ resistance in GBM cells, which may be dependent on the status of MGMT expression. (bvsalud.org)
Main emphasis of Epilepsy Center is to explore mechanisms underlying epileptogenesis, and develop gene- and cell-therapy based treatment strategies for epilepsy. (lu.se)

Mouse ES cells can be put back into a mouse blastocyst and this blastocyst can then be returned to the uterus of a female mouse to develop into a foetus. (eurostemcell.org)
The injected ES cells take part in the development of the foetus and the resulting pup is born with a mixture of cells, (a) from the host blastocyst and (b) cells that came from the injected ES cells. (eurostemcell.org)
Embryonic stem cells are obtained from the inner cells of the blastocyst, an early stage in human embryonic development formed five days after fertilization of the egg by the sperm. (empowher.com)
Embryonic stem cells are derived from the inner cell mass of a blastocyst, which is an accumulation of primordial cells (4-5 days after fertilization). (bvsalud.org)

Self-renewal conditions must prevent the cells from clumping and maintain an environment that supports an unspecialized state. (wikipedia.org)
Stem cells have two unique characteristics: (1) an almost unlimited capacity for self-renewal (they can theoretically divide without limit to replenish other cells for as long as the person is alive) and (2) they retain the potential to produce differentiated and specialized cell types. (erlc.com)
A stem cell is an undifferentiated unit with powerful self-renewal properties that is capable of organizing other cell types in the body. (bvsalud.org)
Stem cells are defined as undifferentiated cells that have precursor properties, are capable of forming many different cell types and have the property of unlimited self-renewal 5 . (bvsalud.org)

I cover mainly cell biology and physiology. (nottingham.ac.uk)
Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)
Dr Paul Verma, Program Leader of MIMR's Stem Cell Biology Program and the Chief Investigator of the Victorian project team, will create the iPS cells for this project. (medicalxpress.com)
So I waited a few weeks, and then went to talk to Dr. Sean Morrison, who is director of the University of Michigan's Center for Stem Cell Biology. (metrotimes.com)
Lund center for Stem Cell Biology and Cell Therapy is one of six Swedish strategic centers of excellence in life sciences, supported by the Swedish Foundation for Strategic Research. (lu.se)

During the past year we have identified the folding patterns of human embryonic stem cells to iPS cells derived from human B cells. (ca.gov)
Briefly, iPS cells derived from human B cells were generated as follows. (ca.gov)
Using an ensemble of computational strategies we found that the majority of genomic regions in iPS cells derived from human B cells was indistinguishable from that of human embryonic stem cells. (ca.gov)
Specifically, we found that approximately 700 genomic regions located throughout the genome showed differential nuclear positioning upon comparing iPS cells derived from human lymphoid cells to that of human embryonic stem cells. (ca.gov)
These data indicate that the majority of the genome derived from that of human embryonic stem cells shows a similar pattern in chromatin folding as compared to that of iPS cells derived from human B cells but that they differ from each other in a subset of genomic regions. (ca.gov)
During the past three years we have identified the folding patterns of human embryonic stem cells to iPS cells derived from human B cells, differentiated progeny and mechanisms that orchestrate the folding patterns of genomes. (ca.gov)

A second important discovery in Sun's study showed that two embryonic stem cells lines derived in similar manners, and therefore expected to behave similarly when differentiating, did not. (sciencedaily.com)

We discuss these properties with examples both from the hematopoietic and embryonic stem cell (ESC) systems. (lu.se)
Bone marrow appears to contain three stem cell populations: hematopoietic stem cells, stromal stem cells and endothelial precursor cells. (bvsalud.org)
Hematopoietic stem cells are cells isolated from blood and bone marrow that can differentiate into a variety of different specialized cells and suffer apoptosis 1 . (bvsalud.org)

Embryonic stem cells are grown from cells found in the embryo when it is just a few days old. (eurostemcell.org)
These laboratory-grown cells are called embryonic stem (ES) cells . (eurostemcell.org)
A stem cell line is a family of constantly dividing cells, the product of a single group of stem cells, which can be grown indefinitely in the laboratory. (erlc.com)
The main claim of embryonic stem cell supporters is that embryonic stem cells can be grown into any of the different types of cells that make up the human body. (thefamuanonline.com)
5. 293T cells - must never be grown to confluency. (lu.se)

The Wisconsin Alumni Research Foundation , eager to make human embryonic stem cell lines available "to as many researchers as possible," last month changed and clarified policies that govern how it licenses the cells. (consumerwatchdog.org)
Banking of multiple cell lines with varying genetic spectrum that can be matched to patients is one possibility. (spiked-online.com)
It might be expected that the richest nation on Earth would encourage its top scientists to pursue this work with vigor rather than limiting funding opportunities, creating legal barriers and fencing off any newly developed cell lines. (spiked-online.com)
However, implementation remains a controversial topic due to diverse views in context to the moral and legal status of early embryo cell lines. (grandviewresearch.com)
The realization that not all human embryonic stem cell lines are born equal is critical," Sun said. (sciencedaily.com)
The expression level of PAX6 reportedly is inversely correlated with the tumorigenicity and invasion of GBM cell lines and is significantly reduced in GBMs ( 7 , 8 ). (spandidos-publications.com)
Currently, iPS cell lines show variability in their potential to produce mature cells. (medicalxpress.com)
Both methods need to be investigated further so we can determine which will produce the most robust cell lines," Dr Verma said. (medicalxpress.com)
Professor Tuch's team will also use their proficiencies in characterising human embryonic stem cell lines and bioinformatics. (medicalxpress.com)
To track differentiating cell populations, reporter cell lines generated by homologous recombination (knock-in) or via BAC transgenes have been widely used in mouse cells (5-8 ). (lu.se)

We plan to scale up the in situ HiC described here to chart the 3D-genomes of human lymphoid and myeloid cells in order to more to determine genomic regions that are differentially localized in the heterochromatic versus euchromatic compartments. (ca.gov)
Genomic alterations in cultured human embryonic stem cells. (nih.gov)
Our results revealed altogether 217 genes and 584 SNPs whose genomic alterations affect the transcriptome in the same cells. (biomedcentral.com)

At the Stem Cell Differentiation and Cytogenetics Group, we are interested in the effects of ionizing radiation on organs such as the brain, the heart, and the lung trying to elucidate the risks for patients treated with ionizing radiation as well as for astronauts, who are inevitably exposed to ionizing radiation on their missions into space. (gsi.de)

The changes, which include new guidelines for company-sponsored academic research and lab-to-lab transfer of cells, have thus far been welcomed by the hESC research community. (consumerwatchdog.org)
The latter clarification coincided with an announcement by CIRM , a non-profit research institute established in early 2005, that it had approved 72 stem cell-related research grants totaling $45 million over two years to 20 academic and non-profit research centers in California. (consumerwatchdog.org)
In fact, the FTCR, along with New York-based watchdog group Public Patent Foundation and Burnham Institute for Medical Research stem cell scientist Jeanne Loring , believe that the patents shouldn't even exist, and have publicly stated that WARF should abandon its claims to them. (consumerwatchdog.org)
Alternatively, research using eggs may point the way to methods which mimic their properties using other human cells and chemical agents. (spiked-online.com)
All research areas are substantiated by analyses (Figure D) monitoring the acute radiation-induced cytogenetic damage and the long-term genetic stability of cells. (gsi.de)
Though not intended to be an exhaustive explanation of this important topic, we believe this will help to clarify and explain the questions most frequently asked about embryonic stem cell research. (erlc.com)
Why are stem cells so important to research? (erlc.com)
There are two main reasons stem cells are of interest to both scientific and medical research. (erlc.com)
What isn't mentioned is the real reason political interest groups are clamoring for the government to invest in embryonic stem cell research. (thefamuanonline.com)
The way supporters of embryonic stem cell research use the pain of the thousands of Americans whose lives have been devastated by Alzheimer's to increase support for funding is obscene. (thefamuanonline.com)
Out of all the diseases that may one day be cured by stem cells the nature of Alzheimer's makes it one of those least likely diseases to be affected by stem cell research, adult or embryonic. (thefamuanonline.com)
And their investigations have overwhelmingly led them to the same conclusion, the true hope for cures of the future lie in adult stem cell research. (thefamuanonline.com)
My research continues to focus on stem cells and osteoblasts and in particular 3D cell culture models and microenvironments. (nottingham.ac.uk)
Victoria and New South Wales have put aside their competitive interstate rivalry to collaborate on a stem cell research project, as announced by Innovation Minister Gavin Jennings and NSW Minister for Science and Medical Research, Verity Firth, today. (medicalxpress.com)
Mr Jennings said changes to the Victorian Infertility Treatment Act and the NSW Human Cloning and Other Prohibited Practices Act have opened up new opportunities for stem cell research in Australia. (medicalxpress.com)
Australia is already a global leader in overall stem cell research and this new and clear regulatory framework gives us an opportunity to extend our leadership into SCNT which could transform how we treat diabetes, heart diseases and Parkinson's," Mr Jennings said. (medicalxpress.com)
Under the rule, a third party could destroy the embryo by taking it apart and preserving the remaining living stem cells for research. (christianitytoday.com)
The prolife lobby also received help from Do No Harm, a coalition of researchers, bioethicists, and doctors who spearheaded a nationwide petition urging Bush to oppose destructive human embryonic stem-cell research. (christianitytoday.com)
Bush's announcement grieved patients' groups and many in the scientific and medical communities who believe embryonic stem-cell research could provide a cure for millions. (christianitytoday.com)
A report published by the nonpartisan Center for Public Integrity (CPI) quoted a National Institutes of Health official who said that "the fledgling stem-cell industry would profit tremendously from federal funding that would cover embryonic stem-cell research. (christianitytoday.com)
I disagree with those who say embryonic stem cell research is not pro-life. (metrotimes.com)
They called for an immediate halt to embryonic research. (metrotimes.com)
Yet he cautioned that 'it is very much premature to suggest that we give up embryonic stem cell research. (metrotimes.com)
Research with these cells has progressed slowly because they are difficult to maintain in culture. (empowher.com)
Embryonic stem cell technology is still at a preliminary research stage and announcements about its potential may be premature. (edu.au)
Experts from around the world are assessing the difficult issue of the extent to which embryonic stem cell research should be allowed to proceed, and to date there is little international consensus on this matter. (edu.au)
How, then, should embryonic stem cell research be regulated in Australia? (edu.au)
This issue was considered by the House of Representatives Standing Committee on Legal and Constitutional Affairs in its report entitled Human Cloning: Scientific, Ethical and Regulatory Aspects of Human Cloning and Stem Cell Research (hereafter the Andrews Report , after the Chair of the Committee, Mr Kevin Andrews, MP) released in September 2001. (edu.au)
In this article we examine embryonic stem cell research and explore the current regulatory framework associated with this research in Australia, with particular reference to the Andrews Report . (edu.au)
Researchers and health professionals consider stem cells to be an important weapon in the fight against diseases, particularly those that have resisted the efforts of science for a long time, and they have become the subject of a great deal of recent research. (bvsalud.org)
Research groups are gathered within five main research clusters: MultiPark, Stem Cell Center and Epilepsy Center. (lu.se)
The possibility to isolate and propagate NSCs and their potential applications in cell therapy have attracted a lot of research interest in recent years. (lu.se)
Bringing together Universities and high school students, UniStem Day is an opportunity to foster learning, discovery and debate in the field of stem cell research - inspiring the scientists of tomorrow. (lu.se)

The "stem cell theory of cancer" states that a subpopulation of cells with stem cell-like properties plays a central role in the formation, sustainment, spread, and drug resistant characteristics of malignant tumors. (medscape.com)

Chimeras can pass on genes from embryonic stem cells to their offspring. (eurostemcell.org)
Researchers can precisely alter the genes of ES cells in the lab, put the cells back into blastocysts, and produce new mice that contain the modified genes. (eurostemcell.org)
We found that a large fraction of genes were differentially localized in human thymocytes derived from embryonic stem cells versus human myeloid cells. (ca.gov)
The ability to manipulate these new cells depended on both the continued presence of LIF and expression of the five genes that are used in reprogramming adult cells into iPSCs. (scienceblog.com)
Two teams of researchers - in Wisconsin and in Kyoto, Japan - had taken ordinary skin cells, transplanted four genes into them and, presto, they suddenly started behaving like embryonic stem cells. (metrotimes.com)

The present study aimed to investigate the potential interaction among TMZ, PAX6 and miRNAs in GBM cells and assess its impact on GBM cell proliferation for, to the best of our knowledge, the first time. (spandidos-publications.com)
The cells carry the potential to cure neurological diseases, diabetes, and many other illnesses. (christianitytoday.com)
Yes, he told me, taking adult skin cells (well, they did use a baby's foreskin in Japan) and getting them to act like embryonic stem cells 'is a very, very important discovery, with enormous potential. (metrotimes.com)
There is hot debate about stem cell use, their ethics and potential for curing diseases. (empowher.com)
Prior to joining Bionics Institute, she completed her PhD at the University of Melbourne investigating the potential of regenerating the auditory nerve using stem cells. (bionicsinstitute.org)
In the article published in the journal Nature on 1 October 2005, the authors say they wrote the article to dispel misconceptions about what stem cells are, what they do, address some controversies surrounding stem cells, and discuss potential uses of stem cells. (asu.edu)
The unique properties of human stem cells have aroused considerable optimism about their potential as new pathways for alleviating human suffering caused by disease and injury. (edu.au)
In view of the importance of the subject, this article provides a review of the literature on studies of stem cells and their potential applications in dentistry. (bvsalud.org)
The overall aim of this thesis has been to assess the potential of autologous grafting in cell replacement therapy for PD. (lu.se)

Genome-wide kinetic properties of transcriptional bursting in mouse embryonic stem cells. (janelia.org)
In this study, we performed single-cell RNA sequencing to analyze the intrinsic noise and mRNA levels for elucidating the transcriptional bursting kinetics in mouse embryonic stem cells. (janelia.org)
They followed exactly the same recipe for making mouse embryonic stem cells, which was published in 1981," Loring said. (consumerwatchdog.org)
Mouse embryonic stem cells are known to be tumorigenic - that is, they can grow uncontrollably and become cancerous. (spiked-online.com)
This new mouse with cells from two different origins is known as a chimera. (eurostemcell.org)
His lab was the first in Australia to create iPS cells in mouse models. (medicalxpress.com)
We had previously found that the growth factors in which mouse stem cells are derived define what those cells can do, and now we've applied those findings to human stem cells. (scienceblog.com)
Robust F1 hybrid embryonic stem cell line from Mus musculus (lab mouse), generated by crossing C56BL/6J mice to Black-Swiss mice. (seattlechildrens.org)
physiologically based animals because a mouse embryonic stem cell line is used. (cdc.gov)

In humans, mice and other mammals, the embryo is a ball of approximately 100 cells at this stage. (eurostemcell.org)
An outer layer of cells, or trophectoderm , will form the placenta that supports the embryo as it grows inside the uterus. (eurostemcell.org)
The process of cell maturation and specialization that would normally take place in the embryo stops. (eurostemcell.org)
Rapid cell division allows the cells to quickly grow in number, but not size, which is important for early embryo development. (wikipedia.org)
The process of obtaining stem cells leads to the destruction of the embryo from which the cells are taken. (erlc.com)
Bush promised in January to review a Clinton administration rule that allowed federal funding for researchers experimenting on embryo cells from fertility clinics. (christianitytoday.com)

Notch1 and Notch 2, but not Notch4, were visualized in the nucleus of EB cells, and all these receptors were also observed as patent cytoplasmic foci. (karger.com)
For example, DNA in living cells is a polymer confined within a nucleus and Chromatin, the term describing DNA complexed with packaging proteins in living cells, is folded and compacted on several length scales. (imsc.res.in)

Electromyography can be used to help determine whether a patient's symptoms stem from birth trama and are therefore not associated with Möbius syndrome. (medscape.com)

These cells are multipotent, meaning they can give rise to several other differentiated and specialized cells of the body (for example, liver cells, kidney cells, brain cells). (erlc.com)

However, much remains to be learned as to how ES cells differ from differentiated progeny. (ca.gov)
In particular, it has remained unclear as to whether and how the 3D-structures of the ES cells differ from that of differentiated progeny and how such differences relate to function. (ca.gov)
Because each mutant has only a few progeny, the overall biological properties of the cell culture are not altered unless a mutation provides a survival or growth advantage. (nih.gov)

Stem cells have the following properties: they self-renew, they can form any cell type in the body and they can multiply into clonal populations. (empowher.com)
Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)

That same year, Thomson published his first paper, "Isolation of a Primate Embryonic Stem Cell Line," in Proceedings of the National Academy of Sciences of the United States of America, detailing the first derivation of primate embryonic stem cells. (asu.edu)

This opens up the possibility of using QDs for fast and accurate imaging applications in stem cell therapy. (nanowerk.com)
Islets organoids would have value in the cell replacement therapy for diabetes apart from usual personalized drug screening routes. (springeropen.com)
The clinical team from Lund headed by Prof. Olle Lindval in collaboration with Prof. Anders Björklund, were the first to show in patients the proof of principle that cell transplantation therapy is feasible approach to treat the patients with Parkinson's disease. (lu.se)
The work with stem cell-based therapy in stroke is in initial phase and recently Dr. Zaal Kokaia started a consortium within EU to develop the preclinical protocol for cell therapy in stroke. (lu.se)

The nature of the stem cell substates and their relationship to commitment to differentiate and lineage selection can be elucidated in terms of a landscape picture in which stable states can be defined mathematically as attractors. (lu.se)

Embryonic stem cells have captured the imagination of scientists and non-scientists alike. (eurostemcell.org)
Scientists have discovered that these nanocrystals can enable researchers to study cell processes at the level of a single molecule and may significantly improve the diagnosis and treatment of diseases such as cancers. (nanowerk.com)
By learning how stem cells differentiate and become specialized, scientists hope to gain a better understanding of how cells in general work and what can go wrong. (erlc.com)
These and many more questions will be answered by PhD students, postdoctoral fellows, and senior scientists from Lund Stem Cell Center during UniStem Day 2024. (lu.se)

miR‑223 and/or PAX6 were overexpressed and knocked down in U251 and U118 cells, and the half maximal inhibitory concentration (IC50) of TMZ and cell proliferation under TMZ treatment were used as measures of TMZ chemoresistance. (spandidos-publications.com)
The results demonstrated that overexpression of miR-223 in GBM cells markedly decreased TMZ-induced inhibition of cell proliferation and increased TMZ IC50, which could be abolished by overexpression of PAX6. (spandidos-publications.com)
On the other hand, knocking down miR‑223 in GBM cells with antagomir significantly enhanced the inhibitory effect of TMZ on GBM cell proliferation and decreased the TMZ IC50, which could be abolished by knockdown of PAX6. (spandidos-publications.com)
In conclusion, the present study demonstrated that TMZ inhibits GBM cell proliferation by inhibiting the expression of miR‑223, which leads to increased expression of tumor suppressor PAX6. (spandidos-publications.com)
The present work introduces a computational implementation of some principles of a theory of organism development, namely that the default state of cells is proliferation and motility, and includes the principle of variation and organization by closure of constraints. (nature.com)
Estrogens are female sex hormones that have genotoxic and proliferation-enhancing effects in cells. (helsinki.fi)

Although they can differentiate into any cell type (except placenta), their growth is not well-controlled, and can provoke an immune reaction. (empowher.com)

A) Colonies of human embryonic stem cells, bright field microscopy, scale bar: 100µm. (gsi.de)

First, stem cells provide a valuable tool for studying both normal and abnormal cellular processes. (erlc.com)
As the first global profiling and annotating of human embryonic stem cell lincRNAs, this work aims to provide a valuable resource for both experimental biologists and bioinformaticians. (biomedcentral.com)
This approach could therefore micromass test, and the embryonic stem cell test (EST) provide a means to reduce the need for animal testing in human risk assessment practices. (cdc.gov)
Finally, we provide protocols for analyzing these cells through flow cytometry and immunocytochemistry. (lu.se)

Human B cells were isolated from human cord blood and transduced with viral vectors expressing Oct4, Sox2, KLF4 and c-Myc. (ca.gov)

We generated light-sensitive human embryonic stem cell (hESC)-derived GABAergic interneurons (hdIN) and tested their functionality. (lu.se)

The main objective of the Stem Cell Center´s Neuroscience Program is to study the properties of neural stem cells (NSCs) and explore the possibilities to use these cells for treatment of neurodegenerative diseases such as Parkinson's disease and stroke. (lu.se)

Instead, the cells multiply to make more undifferentiated cells that resemble the cells of the inner cell mass. (eurostemcell.org)

Anatomy51

Organisms11

Diseases5

Chemicals and Drugs71

Analytical, Diagnostic and Therapeutic Techniques and Equipment55

Psychiatry and Psychology1

Phenomena and Processes62

Disciplines and Occupations5

Technology, Industry, Agriculture1

Humanities1

Information Science2

Embryos20

ESCs19

Pluripotent32

Analysis of human embryonic1

Fetal8

Regenerative7

Adult stem13

HESCs2

Progenitor4

Pluripotency6

Therapeutic5

Mesenchymal stem2

Cardiomyocytes2

19981

Regeneration7

Mammalian3

Ability to differentiate1

Proliferative3

Differentiation of mouse1

Generate6

Neurons21

Tissues5

Tissue19

Neuronal2

Progenitors derived2

Therapies6

Vitro5

Somatic cell nuc2

Rejection2

Developmental2

Genetically3

Gene10

Blastocyst4

Self-renewal4

Biology5

Derived from human6

Expected to behave1

Hematopoietic3

Grown5

Lines10

Genomic3

Organs1

Research33

Tumors1

Genes5

Potential9

Mouse9

Embryo6

Nucleus2

Patient's1

Multipotent1

Progeny3

Populations2

Primate1

Therapy4

Lineage1

Scientists4

Proliferation6

Differentiate into any cell type1

Colonies1

Provide4

Oct41

GABAergic1

Neural1

Resemble1