Promoters tissue gene targeting. Medical search. Frequent questions

A GPC2-targeting antibody-drug conjugate promotes tumor regression in a high-risk neuroblastoma PDX. (aacrjournals.org)
Knowledge of the expression patterns of HE4 in our survey is useful for application in histopathologic diagnosis, and should be taken into consideration in future studies that examine the role of HE4 as a serological tumor biomarker or as a target for gene-based therapy. (nature.com)
A number of PSPs have been defined over the years that include, but not limited to, promoters of prostate-specific antigen (PSA), probasin (PB), mouse mammary tumor virus (MMTV LTR), prostate-specific membrane antigen (PSMA), human glandular kallikrein 2 (hK2), and prostatic steroid-binding protein C3. (hindawi.com)
Transgenic mice bearing PB promoter fused to simian virus 40 large tumor antigen gene all consistently develop prostate adenocarcinoma [ 13 ]. (hindawi.com)
Tumor-normal profiling using massively parallel sequencing targeting 468 genes was performed. (bmj.com)
Assessment of F-MuLV-induced tumorigenesis reveals new candidate tumor genes including Pecam1, St7, and Prim2. (rochester.edu)
Raw areca nut (RAN) consumption induces oral, esophageal and gastric cancers, which are significantly associated with the overexpression of pituitary tumor transforming gene 1/securin and chromosomal instability (CIN). (biomedcentral.com)
Oncolytic virotherapy (OVT) is a promising anti-tumor modality that utilizes oncolytic viruses (OVs) to preferentially attack cancers rather than normal tissues. (biomedcentral.com)
Meanwhile, these non-engineered viruses sometimes inevitably infect and spread to normal tissues, indiscriminately killed both tumor and normal cells, causing a series of unpredicted side effects. (biomedcentral.com)
By the genetic recombination the viruses were conferred with high specificity against tumor cells without targeting normal tissues. (biomedcentral.com)
CONCLUSION: The combination therapy of viral gene transfer and chemotherapy temporarily controls and diminishes tumor burden. (omicsdi.org)
After STAT3 activation, nuclear factor (NF) -κB enter the nucleus, bind to the promoter of the target gene, and induce pro-inflammatory mediators such as InducibleNitric Oxide Synthase (iNOS) , Cyclooxygenase-2 (COX-2) , Tumor necrosis factor-α (TNF-α) and Interleukin-6 (IL-6) ,to reactivate the JAK/STAT3 pathway, thus forming a malignant pro-inflammatory cycle[6]. (researchsquare.com)

The putative targets were cell-surface proteins (or proteins with extracellular epitopes) highly expressed in neuroblastoma compared with normal tissues, and expressed in the majority of tumors. (aacrjournals.org)
GPC2 was highly expressed on the plasma membrane of the majority of neuroblastoma tumors, and its expression was limited in normal tissues, suggesting the possibility for therapeutic targeting with minimal toxicity. (aacrjournals.org)
Here, we used oligonucleotide and tissue microarrays to survey HE4 gene and protein expression in a large group of malignant and normal tissues from adults. (nature.com)

However, the form of enzyme present in the circulation does not correspond to the protein as encoded by the gene: the main fraction of the active enzyme in the circulation has a molecular weight of 730 kD and is probably bound in a complex to other proteins. (wikipedia.org)
Adipose tissue plays an important role in metabolism through its storage and release of triglycerides, peptide hormones (adipokines) and other proteins, and in the case of brown fat, for its role in thermogenesis ( 1 ). (diabetesjournals.org)
The adenovirus early region 1 (E1) gene, which comprises E1a and E1b, encodes the viral early proteins that are necessary for adenoviral replication and the consequent oncolysis of permissive host cells. (hindawi.com)
For example, adenoviral vectors can be used to introduce genes encoding calcium-handling proteins involved in excitation-contraction coupling, which is essential for proper contraction and relaxation of cardiac muscle cells. (aliyuncs.com)
Validation studies - The OmicsLink™ expression ready ORF cDNA clones together with the same ORF clones that contain silent mutations in shRNA target sequence regions can be used for shRNA validation studies and gene/protein functional rescue studies for genes/proteins targeted by corresponding shRNA. (genecopoeia.com)
Recent studies on the involvement of the G12 family of heterotrimeric G proteins (Gα12 and Gα13, the products of the GNA12 and GNA13 genes, respectively) in oncogenic pathways have uncovered a link between G12 signaling and cancer progression. (biomedcentral.com)

It localizes primarily within subnuclear foci associated with the nuclear matrix, and can activate or in some cases repress expression from different promoters. (nih.gov)
MYCN bound to the GPC2 promoter to increase its expression in neuroblastoma, thereby upregulating GPC2 in MYCN -amplified tumors. (aacrjournals.org)
The dependency of neuroblastoma cells on GPC2 expression may prevent the emergence of immune escape mechanisms in response to therapeutic targeting. (aacrjournals.org)
however, the expression in normal and malignant adult tissues has not been examined in detail. (nature.com)
Here, we examined the expression of the HE4 gene and protein in a large series of normal and malignant adult tissues by oligonucleotide microarray and tissue microarray, respectively. (nature.com)
In a series of 175 human adult tumors, gene expression was highest in ovarian serous carcinomas. (nature.com)
Using tissue microarrays and full tissue sections of normal and 448 neoplastic tissues, HE4 immunoreactivity was found in normal glandular epithelium of the female genital tract and breast, the epididymis and vas deferens, respiratory epithelium, distal renal tubules, colonic mucosa, and salivary glands, consistent with HE4 gene expression. (nature.com)
HE4 is one of more than 8900 different human genes represented on U95a GeneChips (Affymetrix, Santa Clara, CA, USA), which we used previously to develop a molecular classification of 175 human carcinomas based on patterns of gene expression. (nature.com)
18 With the exception of the ovarian neoplasms, the same carcinomas analyzed for gene expression were also examined for HE4 by immunohistochemistry on tissue microarrays (see below). (nature.com)
Tissue Expression of Melanoma-associated Antigen A6 and Clinical Characteristics of Gastric Cancer. (nih.gov)
Reducing the levels of the H3K4 demethylase SMCX/Jarid1c in primary neurons reversed down-regulation of key neuronal genes caused by mutant Huntingtin expression. (nih.gov)
This process is mediated by 2 mutually exclusive programs of gene expression: 1) an undifferentiated program supporting proliferation by stem cells within the basal layer and 2) a differentiation program instructing growth arrest and differentiation-associated programmed cell death in suprabasal layers. (stanford.edu)
Epigenetic control of gene expression lasts through multiple cell divisions without alterations in primary DNA sequence and can occur via mechanisms that include histone modification and DNA methylation. (stanford.edu)
Noncoding RNA sequences can regulate gene expression via interactions with epigenetic and other control mechanisms. (stanford.edu)
Expression levels for PART1 in EOC tissue and EOC cell lines were assessed using qRT-PCR. (bvsalud.org)
The results showed that the overexpression of circEpha5 in mouse preantral follicles could increase the expression of Cyp17a1, an androgen synthesis-related gene, which resulted in a higher serum level of testosterone. (bvsalud.org)
Ectopically expressed miR-758-5p reversed the stimulation effects of circEpha5 on steroidogenesis-related gene expression and testosterone release. (bvsalud.org)
The GRM1 coding region remained intact, and 18 of 20 CMFs (90%) showed pronounced (100- to 1400-fold) increases in GRM1 expression levels in comparison with control tissues. (medscape.com)
Thus, E1 protein expression will be confined strictly to the prostate tissues and render this a conditional oncolytic virus (CRAD) within the prostate. (hindawi.com)
By using an expression vector, a 680 bp PSA promoter isolated from a prostate cancer patient demonstrated the prostate-specific expression of the reporter gene [ 9 ]. (hindawi.com)
The rat PB gene promoter (454 bp) contains ARE [ 11 ] and directs a hormonal and developmental regulated expression, of a heterologous gene, specifically to the prostate in transgenic mice [ 12 ]. (hindawi.com)
This involves re-engineering AAVs with improved tissue targeting, finding novel promoters to control gene expression levels, and new cell lines and transfection agents to enhance quality, yields, and scalability. (workinbiotech.com)
Human sodium/iodide symporter (hNIS) is considered as one of the most prevalent nuclear imaging reporter transgenes that provides precise information regarding the kinetics of gene expression, viral biodistribution, toxicity, and therapeutic outcomes using the accumulation of radiotracers at the site of transgene expression. (biomedcentral.com)
Our novel findings provide evidence that inhibition of Hsp90 activity prevents HIV gene expression in replication-competent cellular reservoirs that would typically cause rebound in plasma viremia after antiretroviral therapy cessation. (icem2012.org)
Heat shock has previously been shown to control HIV reactivation from latency (26), and a recent study suggested that Hsp90 inhibitors prevent HIV gene expression by suppressing NF-B activation (27). (icem2012.org)
In this study, we provide novel evidence that 39.5 C accelerates transcription from the HIV promoter through specific inducible host transcription factors and that inhibition of Hsp90 greatly reduces gene expression. (icem2012.org)
Gene expression profiling of ErbB receptor and ligand-dependent transcription. (rochester.edu)
Next, we explored the mechanism of dampened clock-induced IDD and found that knockdown of Bmal1 decreased the expression of nuclear factor erythroid2-related factor 2 (NRF2), a downstream target gene of Bmal1 , and increased inflammatory response, oxidative stress reaction, and apoptosis of NP cells. (biomedcentral.com)
Mammalian parental imprinting represents an exquisite form of epigenetic control regulating the parent-specific monoallelic expression of genes in clusters. (nature.com)
This determined how hierarchical interactions between regulatory elements orchestrate robust parent-specific expression, with implications for non-imprinted gene regulation. (nature.com)
Our work indicates that parental origin of an epigenetic state is irrelevant as long as appropriate balanced gene expression is established and maintained at imprinted loci. (nature.com)
Yet, the intricate form of epigenetic control over the parent-specific expression of multiple genes in an imprinted cluster poses difficulties when trying to decipher the relative contribution of changes in imprinted gene dosage to the resulting physiological phenotypes. (nature.com)
Our results demonstrate that expression of the kn1 gene enhances transformation efficiencies from 3 to 15 fold compared to a control vector, 3-11 fold relative to the highest transformation efficiencies reported for these citrus genotypes. (springer.com)
Background: Expression analysis of estrogen response genes (ERGs) may help to predict the effectiveness of endocrine therapies in breast cancer patients. (iiarjournals.org)
We produced a custom-made, 3-dimensional microarray system (3DMS), using previously identified ERGs, and analyzed expression of ERGs on breast cancer tissues. (iiarjournals.org)
Many investigators consider the expression of estrogen-responsive genes (ERGs) to be evidence of an activated, functional ER ( 6 , 7 ). (iiarjournals.org)
Examination of the expression levels of various ERGs, by alluding to the presence of an activated ER, may identify individual patients who might benefit from treatments targeting the ER. (iiarjournals.org)
This system has already been used successfully in several studies for gene expression and mutation analysis ( 8 , 9 ). (iiarjournals.org)
A set of three expression constructs and a scrambled control is offered against every target gene with the guarantee that at least one of the three will have a knockdown effect of 70% or more on corresponding gene expression as determined by qRT-PCR, otherwise the constructs will be replaced one time free of charge. (genecopoeia.com)
Expression cassettes of all shRNA clones are fully sequenced including the promoter, sense and antisense target sequences, hairpin, terminator, and other linker sequences. (genecopoeia.com)
qRT-PCR - Targeting of the corresponding mRNA by a shRNA expression cassette leads to cleavage and degradation of the mRNA and possible subsequent reduction of protein expression. (genecopoeia.com)
Functional assays by a reporter gene/protein - Alternatively, when the endogenous transcript level of gene of interest is low and/or when qRT-PCR and western blot analysis is not feasible or possible, KD effect can be measured by co-transduction of shRNA clone with an expression clone plasmid which is transcribed into a chimeric mRNA transcript consisting of a reporter gene and the target gene ORF. (genecopoeia.com)
Histopathological evaluation of stomach tissues was performed and securin expression was analysed by immunoblotting as well as by immunohistochemistry. (biomedcentral.com)
Relative expression of miR-17-92 was examined among CRC tumors and noncancerous tissues using quantitative reverse transcription-PCR. (oncotarget.com)
Bioinformatics analysis of the promoter elements of the identified factors suggested NF-κB elements played a role in their enhanced expression, which was tested by chromatin immunoprecipitation. (biomedcentral.com)
Furthermore, the enhanced expression of these secreted factors was found to be facilitated by the activation of their corresponding promoters, where NF-κB seems to be one of the major regulators. (biomedcentral.com)
Impaired control of Interferon-Responding Genes (IRGs) expression by their regulatory mechanisms, including Interferon Regulatory Factors (IRFs), may underlie these findings and it may explain the heterogeneity observed among these conditions. (frontiersin.org)
In the present study we aimed to evaluate the associations between IRF4 gene expression and those of IRGs in SLE and RA patients to gain insight about its links with the IFN signature as well as to explore the potential clinical relevance of these associations. (frontiersin.org)
The gene expression of IRF4 and IRGs (IFI44, IFI44L, IFI6, and MX1) in peripheral blood was analyzed in 75 SLE patients, 98 RA patients, and 28 healthy controls. (frontiersin.org)
The differential expression of IRF4 in RA was then confirmed in publicly available gene expression datasets. (frontiersin.org)
Cluster analysis identified 3 gene expression signatures on the basis of IRF4 and IRGs expression which were differentially used by SLE and RA patients. (frontiersin.org)
The differential expression of IRF4 and IRGs observed in SLE and RA can delineate gene expression signatures associated with clinical features and treatment outcomes. (frontiersin.org)
Signaling through the type I IFN pathway leads to an increased expression of several IFN-responding genes (IRGs). (frontiersin.org)
Type I IFN production is tightly controlled at the gene expression level in a highly ordered process regulated by multiple transcription factors ( 14 ). (frontiersin.org)
1 In particular, morpholino oligonucleotides (MOs) have been used to block the expression of targeted genes in several invertebrate and vertebrate models. (cdc.gov)
Evaluation of epigenetic-related gene expression (DNMT, HDAC1) in Iranian patients with systemic lupus erythematosus. (cdc.gov)
3. Translational research - to identify predictive biomarkers for treatment decision - by immunohistochemistry, gene expression or sequencing. (lu.se)

The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. (nih.gov)
RAN-mediated pRb-inactivation induced securin upregulation, a putative E2F1 target, by inducing misregulation in chromatin remodeling in its promoter region, which led to transcriptional activation and subsequent development of chromosomal instability. (biomedcentral.com)
These findings indicate that the rs9588884 GG and the rs982873 CC in the promoter of miR-17-92 may protect against CRC, possibly by decreasing transcriptional activity and eventually resulting in lower levels of miR-20a and miR-17. (oncotarget.com)
microRNAs (miRNAs) are a class of non-coding RNAs, ~23 nucleotides in length, which function as post-transcriptional gene regulators by repressing protein translation or accelerating degradation of target messenger RNAs (mRNAs) [ 1 , 2 ]. (oncotarget.com)

Virus-mediated gene therapy has the potential to deliver exogenous genetic material into specific cell types to promote survival and counteract disease. (frontiersin.org)
These new models, which we term Multi-Functional Human Tissue Genetics, allow up to 10 alleles or more to be altered simultaneously, permitting genetic experiments with an unprecedented degree of rapidity and complexity. (stanford.edu)
Improving specificity through genetic modifications or utilizing tissue-specific promoters can enhance targeting efficiency while minimizing off-target effects. (aliyuncs.com)
The effects of a maize knotted1 ( kn1) gene under the control of the cauliflower mosaic virus 35S promoter on genetic transformation efficiencies of six citrus genotypes were tested. (springer.com)
With all six genotypes of citrus tested including Eureka lemon, a cultivar difficult to be transformed, our results demonstrate that the kn1 gene may provide an effective molecular tool to enhance genetic transformation efficiencies of various citrus varieties. (springer.com)
On the other hand, transgenic plants produced from mature tissue via genetic transformation can drastically shorten flowering and fruiting time and thus speed up field evaluation studies. (springer.com)
Targeting PAR1 through genetic deficiency, small interfering RNA (siRNA) knockdown, or pharmacological blockade decreases itch and skin damage caused by V8 and S. aureus exposure. (iasp-pain.org)

A study in Nature suggests epitope editing in donor stem cells prior to bone marrow transplants can stave off toxicity when targeting acute myeloid leukemia with immunotherapy. (genomeweb.com)

Conditional gene targeting has been extensively used for in vivo analysis of gene function in adipocyte cell biology but often with debate over the tissue specificity and the efficacy of inactivation. (diabetesjournals.org)
To directly compare the specificity and efficacy of different Cre lines in mediating adipocyte specific recombination, transgenic Cre lines driven by the adipocyte protein 2 (aP2) and adiponectin (Adipoq) gene promoters, as well as a tamoxifen-inducible Cre driven by the aP2 gene promoter (iaP2), were bred to the Rosa26R (R26R) reporter. (diabetesjournals.org)
In this study, we have analyzed and compared three prostate-specific promoters (PSA, probasin, and MMTV LTR) for their specificity and activity both in vitro and in vivo . (hindawi.com)
With the leap of gene cloning in the molecular virology, the scientists focus on improvement of their antitumor specificity and efficiency by manipulating the viral genomes. (biomedcentral.com)

They analyzed the whole-genome bisulfite sequencing profiles of 45 esophageal tumors, representing both the squamous cell carcinoma and adenocarcinoma subtypes, and matched nonmalignant tissue. (genomeweb.com)
Immunotherapeutic GPC2 targeting may be beneficial in high-risk neuroblastoma and other embryonal tumors. (aacrjournals.org)

This gene encodes a member of the Sp subfamily of Sp/XKLF transcription factors. (nih.gov)
The HE4 (WFDC2) gene encodes a WAP-type four disulphide core domain-containing protein with a presumptive role in natural immunity. (nature.com)
The maize homeobox gene knotted1 ( kn1 ) encodes an AP2/ERF transcription factor (Hake et al. (springer.com)
The reporter gene encodes a reporter protein enzyme that can be used in functional assays. (genecopoeia.com)

We are currently pursuing studies of the dominant signaling and gene regulatory networks that control this process, including the Ras/MAPK cascade, which is required for stem cell-mediated self-renewal and the p53 transcription factor family member, p63, which is required for epidermal differentiation. (stanford.edu)
BMAL1 is a core clock transcription factor, which promotes the transcription of downstream clock-controlled genes (CCGs) by targeting the E-box structure in their promoter region to regulate the physiological functions of the body. (biomedcentral.com)
This gene product is involved in cell cycle progression, p53-mediated apoptosis, transcription activator of several other oncogenes [ 4 ] and DNA repair [ 5 ]. (biomedcentral.com)
Microarray analysis of distinct gene transcription profiles in non-eosinophilic chronic sinusitis with nasal polyps. (southernbiotech.com)
The P2-promoter of Mdm2 carries two distinct p53 response elements wherein stress induced p53 binds and promotes transcription of Mdm2 from this alternative promoter [ 9 , 10 ]. (oncotarget.com)

High transformation efficiency of citrus is of great importance for large scale characterization of gene functions and also cultivar development via transgenic and genome editing technologies. (springer.com)
Another challenge to using transgenic plant technology in citrus is the long juvenile phase of immature tissues that ranges from 5 to 20 years, which greatly delays analysis of the effects of candidate genes. (springer.com)
the invention also provides non-human animals and cells comprising a transgene encoding an APP comprising the Swedish mutation and further comprising functionally disrupted endogenous APP gene loci, transgenes and targeting constructs used to produce such transgenic cells and animals, transgenes encoding human Swedish mutation APP polypeptide sequences, and methods for using the transgenic animals in pharmaceutical screening and as commercial research animals for modeling neurodegenerative diseases (e.g. (justia.com)
In combination with transgenic organisms, such enzyme-actuated antisense tools will enable gene silencing in specific cell types, including tissues that are not amenable to optical targeting. (cdc.gov)
Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)

E1-deleted (including E1a-deleted) adenoviruses are replication defective and are commonly used as viral vectors to carry therapeutic genes for gene therapy. (hindawi.com)
Adenoviruses are DNA viruses that can be genetically modified to deliver therapeutic genes into target cells, offering a means to correct or replace defective genes responsible for disease pathogenesis. (aliyuncs.com)
By enhancing blood supply to the damaged heart tissue, these therapeutic genes can potentially improve cardiac function and reduce symptoms such as shortness of breath and fatigue. (aliyuncs.com)

Using an RNA-sequencing (RNA-seq)-based pipeline to analyze 126 primary high-risk neuroblastomas, Bosse and colleagues identified candidate genes for optimal immunotherapeutic targeting. (aacrjournals.org)

The exogenous promoter and chimeric ADAM9 coding sequence containing human extracellular domain, mouse transmembrane domain and cytoplasmic domain was inserted to replace murine exon 5-6. (biocytogen.com)

ChIP-qPCR assays were performed to evaluate the recruitment of different histone modifications in the core promoter region of securin gene as well as its upstream and downstream regions. (biomedcentral.com)
ChIP-qPCR data revealed that the quantity of DNA fragments retrieved from the immunoprecipitated samples was maximum in the -83 to -192 region than further upstream and the downstream of the promoter for H3K4Me3, H3K9ac, H3K18ac and H3K9me3. (biomedcentral.com)
After wholemount clearing and imaging, samples processed with EZ Clear can be subjected to downstream applications, such as tissue embedding and cryosectioning followed by standard histology or immunofluorescent staining without loss of fluorescence signal from endogenous or synthetic reporters. (elifesciences.org)

By harnessing these viral vectors' ability to efficiently infect both dividing and non-dividing cells, scientists can introduce specific genes into diseased tissues and initiate targeted cellular responses aimed at restoring normal physiological functions. (aliyuncs.com)

Administration of gene therapy viruses into skeletal muscle, where distal terminals of motor and sensory neurons reside, has been shown to result in extensive transduction of cells within the spinal cord, brainstem, and sensory ganglia. (frontiersin.org)
In this review article, we outline key characteristics of major gene therapy viruses-adenovirus, adeno-associated virus (AAV), and lentivirus-and summarize the mechanisms regulating important steps in the virus journey from binding at peripheral nerve terminals to nuclear delivery. (frontiersin.org)
Gene therapy viruses are non-replicating, but still hijack host cell machinery to express transgenes of interest in the nucleus. (frontiersin.org)
Similarly, direct injection of viruses into the cerebrospinal fluid (e.g., via lumbar puncture in humans) also permits targeting of the peripheral (PNS) and central nervous systems (CNS). (frontiersin.org)
Therefore, strategies to use conditional oncolytic virus, or the so-called attenuated replication-competent viruses, to specifically target prostate tissue have been developed [ 2 - 4 ]. (hindawi.com)
Affinia Therapeutics is enhancing gene therapies by expanding the potential of adeno-associated viruses (AAVs). (workinbiotech.com)
These noninvasive visualization systems provide real-time follow-up of viral progression within the cancer tissue by the ability of engineered oncolytic viruses (OVs) to encode reporter transgenes based on recombinant technology. (biomedcentral.com)

Therefore, targeting this epigenetic signature may be an effective strategy to ameliorate the consequences of HD. (nih.gov)
Mammalian parental imprinting is a form of epigenetic regulation that causes genes to be expressed from only one chromosome homolog according to parent-of-origin 1 , 2 . (nature.com)
Since the molecular mechanism underlying securin upregulation remains unclear, this study intended to investigate the association of securin upregulation with the Rb-E2F1 circuit and epigenetic histone (H3) modification patterns both globally and in the promoter region of the securin gene. (biomedcentral.com)

The destruction of the target gene mRNA by targeting shRNA results in the degradation of the reporter gene and, therefore, translation reduction of reporter protein enzyme. (genecopoeia.com)

For the microarray analysis, we surgically obtained 27 breast cancer tissue samples from patients undergoing treatment at the Saitama Cancer Center Hospital (Saitama, Japan) from 25/8/2003 - 6/11/2003. (iiarjournals.org)

The times below were that site-specific selection of self-esteemproblems has on the survival of the & of splines so that higher gene strains and larger promoter saponins show more adjacent biomarkers. (scoutconnection.com)

We and others have synthesized light-activatable cMOs that allow spatiotemporal control of RNA splicing or translation, complementing the use of conditional knockouts to study stage- and tissue-specific differences in gene function. (cdc.gov)

Luciferase reporter gene assay was also performed to investigate biological functions of PART1, miR-150-5p and MYB in EOC, and an animal xenograft model was employed to test tumorigenicity. (bvsalud.org)
Dual-luciferase reporter gene studies identified miR-758-5p as a direct target of circEpha5. (bvsalud.org)
Moreover, we describe different types of reporter genes and their potency in the clinical trials. (biomedcentral.com)
Vectors with mCherry or eGFP reporter genes for monitoring transfection or transduction efficiencies. (genecopoeia.com)

To address this and to also study the oncogenic potential of unregulated function of dominant regulators of epithelial homeostasis noted above, we developed Multi-Functional Human Tissue Genetics noted above which, when combined with skin tissue regeneration on immune deficient mice, has permitted the molecular reconstruction of events sufficient to trigger human cancer. (stanford.edu)

We observed gene-specific changes in histone H3 lysine 4 trimethylation (H3K4me3) at transcriptionally repressed promoters in R6/2 mouse and human HD brain. (nih.gov)

In this study, we found that the circadian clock of NP cells was totally disrupted by knockdown of the core clock gene brain and muscle arnt-like protein-1 ( Bmal1 ), which thus induced the dysfunction of NP cells. (biomedcentral.com)
shRNA is driven by pol III promoters (H1, U6) or pol II promoters (CMV, EF1a, CAG and CBh), both with great knockdown efficiency. (genecopoeia.com)
Functional assays - The shRNA knockdown effect can be studied by end-point biological and biochemical assays such as cell proliferation, colony formation, cell cycle analysis, and migration based on the potential role played by each gene /protein. (genecopoeia.com)

Another approach involves delivering genes that regulate myocardial contractility, aiming to improve the heart's pumping ability. (aliyuncs.com)

AAVs are the vectors that target cells and deliver gene therapies. (workinbiotech.com)
Affinia is using their Affinia Rationally-designed Therapies (ART) platform to improve the efficacy, safety, and manufacturability of AAVs for gene therapies. (workinbiotech.com)
Conclusion: 3DMS may be useful for accurately predicting prognosis and whether endocrine therapies targeting the ER would be effective in an individual breast cancer patient. (iiarjournals.org)

Our experimental focus is on the mammalian setting using multiomics, informatics, mouse genetics, human genetics, single cell studies, and new human tissue platforms. (stanford.edu)

The insertion disrupts the endogenous murine ADAM9 gene, resulting in a non-functional transcript. (biocytogen.com)

The former was first found in fetal tissue, the second was found to be more abundant in adult tissue, and initially they were termed fetal and adult thymidine kinase. (wikipedia.org)
Transplantations of fetal tissue in the 1980s and 1990s provided proof-of-concept for the potential of cell replacement therapy for PD and some patients benefitted greatly from their transplants. (lu.se)

In addition to classical gene regulatory networks noted above, we have recently identified a central role for additional biologic mechanisms, namely gene regulation by chromatin regulators and by noncoding RNAs. (stanford.edu)
Adenoviral vectors have shown promise in gene therapy for chronic heart failure by targeting key cellular mechanisms involved in the progression of this condition. (aliyuncs.com)

The PSA promoter has an androgen response element (ARE) and its activation requires both the androgen receptor (AR) and its ligand, androgen, to be present. (hindawi.com)

DNA methylation status of SHATI/NAT8L promoter in the blood of cigarette smokers. (cdc.gov)
A DNA methylation signature in the stress driver gene Fkbp5 indicates a neuropathic component in chronic pain. (cdc.gov)
Integrative Analysis Unveils the Correlation of Aminoacyl-tRNA Biosynthesis Metabolites with the Methylation of the SEPSECS Gene in Huntington's Disease Brain Tissue. (cdc.gov)

While these vectors have a broad tropism for infecting various cell types, non-specific uptake by off-target tissues can lead to unintended side effects. (aliyuncs.com)
Activated ER binds to estrogen-responsive elements (EREs), which are located in the promoter regions of various target genes. (iiarjournals.org)
Validated qRT-PCR primers for corresponding target genes (and any other published transcripts) are also available. (genecopoeia.com)
There is a compelling body of evidence linking the type I IFNs and the presence of the IFN signature to systemic autoimmune conditions in peripheral blood and target tissues ( 4 - 6 ). (frontiersin.org)
Tissues with complex three-dimensional morphology, significant depth, or rapid movement are challenging to selectively target by optical techniques. (cdc.gov)

While imprinting perturbations are widely associated with developmental abnormalities, the intricate regional interplay between imprinted genes makes interpreting the contribution of gene dosage effects to phenotypes a challenging task. (nature.com)

Although virus-based gene therapy is a promising strategy to combat advanced prostate cancer, its current effectiveness is limited partially due to inefficient cellular transduction in vivo . (hindawi.com)
By scavenging free radicals, Jobelyn helps protect our tissues from damage and supports overall cellular health. (healthforeverng.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)

Because ChR2 and NpHR are genetically targetable, we describe the use of this technology to control the electrical activity of specific populations of neurons ( i.e. , pyramidal neurons) embedded in heterogeneous tissue with high temporal precision. (jove.com)
This approach could be potentially applied directly in the brain by targeting resident cells as a source of new neurons. (lu.se)

The idea behind this study is to place the Ad5 E1 region in cis complementation (i.e., use E1 as a transgene) back into an E1-deleted, replication-defective adenovirus under the control of a prostate-specific promoter (PSP). (hindawi.com)
Single gene down-regulation - The knocking down (KD) effect of the shRNA clones for a single gene can be studied and compared with that of a scrambled nucleotide control clone which is included for free with every shRNA clone order. (genecopoeia.com)

Jobelyn achieves this by stimulating various apoptosis promoter genes while downregulating certain apoptosis inhibitor genes, effectively targeting cancer cells. (healthforeverng.com)
The aim of this study was to investigate the association of polymorphisms in the promoter region of miR-17-92 cluster with the risk of colorectal cancer (CRC). (oncotarget.com)
Cancer Biology: targeting therapy to maximize benefit and minimize side effects - What are VEGF, erbb2 and BCR-ABL? (powershow.com)
These events lead to the persistence of colonic mucosal inflammation, hinder mucosal regeneration, lead to tissue damage, and eventually lead to the development of the disease in the direction of inflammation-cancer transformation[7]. (researchsquare.com)
genic models are inadequate for number of activated CD8-positive T LMP1 was strongly expressed in the understanding the cancer etiology in cells increased considerably in the lymphoma tissues but was hardly the context of natural viral infection. (who.int)

Evaluating genomic signatures of aging in brain tissue as it relates to Alzheimer's disease. (cdc.gov)

Indeed, for sever- with histopathological findings and ulin heavy chain promoter/enhancer. (who.int)

This route is minimally invasive and therefore clinically relevant for gene therapy targeting to peripheral nerve soma. (frontiersin.org)
With thousands of clinical trials to date, gene therapy is a flourishing strategy with great promise for the treatment of diseases impacting the nervous system. (frontiersin.org)
In recent years, the field of regenerative biomedicine has witnessed a paradigm shift with the emergence of gene therapy as a promising treatment modality. (aliyuncs.com)
One particularly influential approach in this regard is the use of adenoviral vectors, which hold great potential for unlocking the therapeutic benefits of gene therapy. (aliyuncs.com)
Gene therapy using adenoviral vectors offers an innovative solution for individuals like John who face debilitating conditions that have traditionally been difficult to manage effectively. (aliyuncs.com)
With further progress in this field, gene therapy using adenoviral vectors may become a transformative approach in improving patient outcomes and quality of life for individuals like John. (aliyuncs.com)
This vision is becoming increasingly plausible with the advent of gene therapy, a promising field that aims to manipulate an individual's genes to prevent or treat disease. (aliyuncs.com)
Human Gene Therapy. (kcl.ac.uk)
METHODS: In a non-randomized pilot trail we tested whether a local therapy based upon an adenoviral gene transfer of wild type p53 in combination with gemcitabine administration would be safe in patients with liver metastases due to pancreatic carcinoma. (omicsdi.org)

Replication-competent HIV was isolated from the mouse spleens despite undetectable HIV RNA or infected cells in the peripheral blood, indicating the establishment of a persistent tissue reservoir. (icem2012.org)

Additionally, we describe how neuropathology can negatively influence these pathways, and conclude by discussing opportunities to optimize the intramuscular administration route to maximize gene delivery and thus therapeutic potential. (frontiersin.org)
Pathway analysis - Genes have been grouped into various signal transduction, metabolic, and disease pathways and associations, as well as gene families and groups. (genecopoeia.com)

Replication-competent virus was detected in spleen cells from these nonviremic Hsp90 inhibitor-treated mice, indicating the presence of a tissue reservoir of persistent contamination. (icem2012.org)

Novel Mutation (Gly212Val) in the PS2 Gene Associated with Early-Onset Familial Alzheimer's Disease. (nih.gov)

These findings demonstrate that direct targeting of GRM1 is a necessary and highly specific driver event for the development of CMF. (medscape.com)

amyloid peptide, a major constituent of the amyloid plaque, can result from mutations in the gene encoding amyloid precursor protein, a protein which when normally processed will not produce the .beta. (justia.com)
We tested this paradigm in a genetically engineered mouse model in which the feedback loop was disrupted by point mutations in the p53 binding site of the Mdm2 promoter. (oncotarget.com)
If this is the case, gene-correction presents a solution for patients with known monogenetic mutations. (lu.se)

However, efforts have been made to genetically transform mature citrus tissues but the efficiencies are generally extremely low (Marutani-Hert et al. (springer.com)

The manuscript reports a new tissue clearing procedure that is faster (clearing within 48 hours), uses less hazardous chemicals, and importantly appears to result in less tissue volume change compared to other methods. (elifesciences.org)
The simple protocol adds further to the toolbox of tissue clearing methods and is one that is likely to be even more popular than many current methods. (elifesciences.org)

Their appearance can mimic that of fibrous tissue or hyaline cartilage. (medscape.com)
GALNS deficiency induces the accumulation of glycosaminoglycans (GAGs), keratan sulfate (KS) and chondroitin-6-sulfate (C6S) in multiple tissues, particularly bone, cartilage, heart valves, and cornea, whereas β-galactosidase deficiency induces the accumulation of only KS in those tissues. (medscape.com)

Genes for virus specific thymidine kinases have been identified in Herpes simplex virus, Varicella zoster virus and Epstein-Barr virus. (wikipedia.org)

The Adipoq-Cre exhibited no observable recombination in any other tissues examined, whereas both aP2-Cre lines resulted in recombination in endothelial cells of the heart and nonendothelial, nonmyocyte cells in the skeletal muscle. (diabetesjournals.org)
A GPC2-targeting antibody-drug conjugate was developed and was cytotoxic to neuroblastoma cells expressing GPC2 in vitro . (aacrjournals.org)
29) analyzed Bis(s) zum effects for the amorphous gene and were how DNA guided in proportional cells of joint and possible protons performed to outcomes of important pairs generally in intracellular results. (scoutconnection.com)
In this study, we used IL-1β, a commonly used inflammatory factor, to induce IDD and found that the IVD clock was dampened in degenerated human nucleus pulposus specimens, rat nucleus pulposus (NP) tissues, and cells. (biomedcentral.com)
In addition, NRF2 activation attenuated the dysfunction of NP cells induced by the dampened IVD clock and the degenerative process of NP tissues in an organotypic tissue-explant model. (biomedcentral.com)
Gene targeting strategy for B-hADAM9 MC38 cells. (biocytogen.com)
Upregulation of miR-22 alleviates oxygen-glucose deprivation/reperfusion-induced injury by targeting Tiam1 in SH-SY5Y cells. (cdc.gov)
However, post-mortem analysis of transplanted tissue revealed accumulation of pathological Lewy bodies in a small subset of transplanted cells over time, revealing a host-to-graft disease propagation. (lu.se)

The genes of the two human isoenzymes were localized in the mid-1970s. (wikipedia.org)
OmicsLink ™ shRNA clone collections include lentiviral and non-viral vector-based shRNA constructs against genome-wide human, mouse and rat genes. (genecopoeia.com)

The MAGEA genes are clustered at chromosomal location Xq28. (nih.gov)

[ 12 ] They found that the glutamate receptor gene GRM1 recombines with several partner genes through promoter swapping and gene fusion. (medscape.com)

Anatomy18

Organisms13

Diseases3

Chemicals and Drugs105

Analytical, Diagnostic and Therapeutic Techniques and Equipment29

Phenomena and Processes95

Information Science3

Health Care1

Tumor12

Normal tissues3

Proteins6

Expression57

Transcriptional4

Genetic7

Toxicity1

Specificity4

Tumors2

Encodes4

Transcription5

Transgenic5

Therapeutic genes3

Candidate genes1

Chimeric1

Downstream3

Viral vectors1

Viruses7

Epigenetic3

Degradation1

Microarray1

Biomarkers1

Conditional1

Reporter4

Oncogenic1

H3K4me31

Knockdown3

Regulate1

Therapies3

Mammalian1

Endogenous1

Fetal tissue2

Mechanisms2

Androgen1

Methylation3

Target5

Phenotypes1

Cellular3

Neurons2

Control2

Cancer5

Genomic1

Histopathological1

Therapy9

Peripheral blood1

Pathways2

Mice1

Mutation1

Findings1

Mutations3

Genetically1

Methods2

Cartilage2

Thymidine1

Cells8

Human2

Chromosomal1

Receptor1