• Recent reports have shown that the sensitivity of non-small-cell lung cancer cell lines to EGFR inhibitors such as erlotinib (Tarceva, OSI Pharmaceuticals) is dependent on inhibition of the phosphatidylinositol 3′-kinase-phosphoinositide-dependent kinase 1-Akt-mTOR pathway. (aacrjournals.org)
  • Therefore, inhibiting EGFR alone may not be sufficient for substantial inhibition of all tumor cells, highlighting the need for multipoint intervention. (aacrjournals.org)
  • Therefore, in select cell lines, inhibition of both S6 and Akt was achieved only with the combination of erlotinib and rapamycin. (aacrjournals.org)
  • More importantly, we show that MESF treatment leads to significant inhibition of tumor growth and a 3-fold increase in the life span of tumor bearing animals compared to untreated tumor mice. (nature.com)
  • Finally, MCT4 inhibition mitigates the induction of angiogenesis, suggesting that MCT4 also plays a crucial role in tumor-related angiogenesis. (hindawi.com)
  • For this purpose, we designed an MCT4 overexpression and knockdown/inhibition model to explore the functional consequences of MCT4 expression on cancer cell metabolism, survival, proliferation, migration, invasion potential, and angiogenesis as well as on cell cycle profiles and cell death mechanisms. (hindawi.com)
  • Inhibition of EHMT2 expression or activity in human ERMS cell lines reduced DKK1 expression and elevated canonical Wnt signaling resulting in myogenic differentiation in vitro and in mouse xenograft models in vivo. (elifesciences.org)
  • Alanine transition of S63 and S262 (S63A and S262A) of IκBα induced inhibition of cell proliferation and suppression of p65 transcription activity. (researchsquare.com)
  • My results showed promise in improving the efficacy of doxorubicin (a chemotherapy drug), inhibition of cancer cell proliferation, and hampering cancer cell migration. (brown.edu)
  • 1) For example, the down-regulation of miR15s and miR - 16 - 1 in chronic lymphocytic leukaemia, prostate cancer and pituitary tumours, leading to the inhibition of tumour growth and induce cell cycle arrest at the G 1 - phase by target cell cycle regulators ( cyclin D1, cyclin E1, cyclin D3 and CDK6). (ukessays.com)
  • Gint4.T aptamer is able to specifically bind to the human PDGFRβ ectodomain (Kd: 9.6 nmol/l) causing a strong inhibition of ligand-dependent receptor activation and of downstream signaling in cell lines and primary cultures of human glioblastoma cells. (unina.it)
  • We could confirm the increased polyploidy upon Mps1 inhibition in neuroblastoma cell lines and PDX model, leading to mitotic catastrophe activating the caspase-dependent mitochondrial apoptotic pathway. (lu.se)
  • Some of the new approaches depend on tumor biology and aim specifically to inhibit tumor growth and metastasis by targeting the tumor microenvironment or vasculature (leaving normal cells unaffected) or focusing on specific protein or signal transduction pathways. (medscape.com)
  • Overexpression of this gene is associated with tumor cell proliferation and metastasis. (cancerindex.org)
  • DRAM2 was preferentially upregulated in NSCLC tissues and higher expression of DRAM2 in NSCLC correlated with tumor node metastases stage and lymph node metastasis. (cancerindex.org)
  • Additionally, DRAM2 overexpression promoted cell metastasis and proliferation in vitro, while knockdown of DRAM2 expression yielded opposite result. (cancerindex.org)
  • Breast cancer stem cells (BCSCs) play a critical role in chemoresistance, metastasis, and poor prognosis of breast cancer. (hindawi.com)
  • is known to exert anticancer effects, such as inducing cell cycle arrest, inhibiting metastasis, and overcoming immunotherapy resistance in breast cancer cells. (hindawi.com)
  • Our scientists pursue every aspect of cancer research-from exploring the biology of genes and cells, to developing immune-based treatments, uncovering the causes of metastasis, and more. (mskcc.org)
  • Sphingolipids are modified in multiple cancers and are responsible for tumor proliferation, progression, and metastasis. (frontiersin.org)
  • Previous studies have reported that LINC00673 can promote epithelial-mesenchymal transition (EMT), proliferation and metastasis in thyroid carcinoma [ 5 ] . (jcancer.org)
  • Overexpression of LINC00673 promotes invasion and metastasis which associated with a poor prognosis in tongue squamous cell carcinoma [ 6 ] . (jcancer.org)
  • Scientists have identified a distinct subpopulation of cancer stem cells (CSCs) that is responsible for metastasis of a deadly human pancreatic cancer. (news-medical.net)
  • Mutations in the cell adhesion molecule known as integrin alpha 7 (integrin 7) lead to unchecked tumor cell proliferation and a significantly higher incidence in cancer spread, or metastasis, in several cancer cell lines, report researchers at the University of Pittsburgh School of Medicine in a study published in the Journal of the National Cancer Institute. (news-medical.net)
  • Overexpression of cyclophilins has been observed in HCC increasing proliferation, metastasis and promoting chemoresistance. (lu.se)
  • Unexpectedly, we found that their depletion in primary human lung fibroblasts failed to induce cell cycle arrest but strongly suppressed cell cycle progression. (nih.gov)
  • We show that the effects on cell cycle progression stemmed from reduced ribosome content and translational capacity, which suppressed the accumulation of cyclins at the translational level. (nih.gov)
  • Low AKAP12 expression in a larger cohort of patients (75) characterized tumor invasiveness, recurrence, and progression, indicating its potential as a prognostic biomarker. (nature.com)
  • MicroRNA-16-5p was a tumor suppressor in the progression of glioma. (ijpsonline.com)
  • The tumor microenvironment (TME) promotes BCSC survival and cancer progression, and targeting the TME can aid in successful immunotherapy. (hindawi.com)
  • T he presence and activity of various proteins are required in the cell cycle progression. (ukessays.com)
  • HNF1A inhibited cell progression in pancreatic cancer cells. (jcancer.org)
  • However, binding of p16(INK4A) blocks CDK4's or CDK6's ability to stimulate cell cycle progression. (medlineplus.gov)
  • This family, which includes epidermal growth factor receptor (EGFR), plays a pivotal role in normal cell growth, lineage determination, repair, and functional differentiation. (medscape.com)
  • Oncostatin M inhibits proliferation of rat oval cells, OC15-5, inducing differentiation into hepatocytes. (joplink.net)
  • Our study demonstrates that ERMS cells are vulnerable to EHMT2 inhibitors and suggest that targeting the EHMT2-DKK1-β-catenin node holds promise for differentiation therapy. (elifesciences.org)
  • To identify potential therapeutic targets, our lab screens the genes and microRNAs in these signatures for involvement in proliferation, differentiation, and survival of sarcoma cell lines. (mskcc.org)
  • Let-7 Let-7 has an essential role in cell cycle and the differentiation of cell cycle terminals. (ukessays.com)
  • Adherent brain cancer cells cultured in fetal bovine serum (FBS) undergo unwanted phenotype changes and cell differentiation, while culturing in 3D neurospheres in serum-free conditions maintains the original properties of the primary tumor (1). (sigmaaldrich.com)
  • Moreover, Gint4.T aptamer drastically inhibits cell migration and proliferation, induces differentiation, and blocks tumor growth in vivo. (unina.it)
  • Growth differentiation factor 15 overexpression promoted OSCC cell proliferation through erbB2 phosphorylation, as well as downstream AKT and Erk signaling pathways. (bvsalud.org)
  • Growth differentiation factor 15 overexpression promotes OSCC proliferation through erbB2 phosphorylation. (bvsalud.org)
  • Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
  • We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
  • Temporal multimodal single-cell profiling of native hematopoiesis illuminates altered differentiation trajectories with age. (lu.se)
  • Targeted molecular therapy against EGFR has shown promise as an adjuvant therapy in preliminary studies in several solid tumors, including head and neck cancer. (medscape.com)
  • In line with this observation, elevated extracellular lactate concentrations up to 40 mM and corresponding low extracellular pH (pH e ) have been found in solid tumors under hypoxic conditions [ 18 - 22 ]. (hindawi.com)
  • Activation of Akt leads to the stimulation of antiapoptotic pathways, promoting cell survival. (aacrjournals.org)
  • With the increased understanding of molecular mechanisms and basic pathways in the pathogenesis of squamous cell cancer of the head and neck , these pathways may be modified, and rational approaches in cancer therapy at the molecular level may be created. (medscape.com)
  • Thus, GDF15-overexpressing OSCC tumors are sensitive to TPF chemoagents through caspase-9-dependent pathways. (bvsalud.org)
  • Several pathways involved in cell cycle and DNA repair were affected upon NV651 treatment. (lu.se)
  • Taken together we have shown that LTD4 might have an impact on tumour development through the regulation of CysLT1R and its downstream signalling pathways. (lu.se)
  • Considering that intratumoral estrogen metabolism plays an important role, our co-culture systems provide an environment similar to that of the tumor in living patients in terms of metabolism and synthesis of intratumoral estrogens. (scirp.org)
  • Besides, upregulated MCT4 has been linked to altered tumor metabolism as well as to increased growth and survival in breast and pancreatic cancer [ 33 , 34 ]. (hindawi.com)
  • The RAC serine/threonine-protein kinase (AKT) family of serine/threonine protein kinases, particularly the AKT1 isoform, has been identified abnormally expressed in hepatocellular carcinoma (HCC) cells, and is highly associated with cell behavior, including proliferation, survival, metabolism, and tumorigenesis. (spandidos-publications.com)
  • Scientists working in basic, translational, and clinical cancer metabolism research are invited to join the Academy in New York on April 17th to discuss the intersection between cell signaling and metabolism. (nyas.org)
  • Cancer Cell Metabolism: Unique Features Inform New Therapeutic Opportunities. (nyas.org)
  • This characteristic reduction in pH is caused by altered cancer cell metabolism, greater diffusion distances to vasculature, and lack of buffer in the extracellular space. (brown.edu)
  • Thus, unlike other tumor suppressors, RPL5/RPL11 play an essential role in normal cell proliferation, a function cells have evolved to rely on in lieu of a cell cycle checkpoint. (nih.gov)
  • These are divided into two group, those miRNAs that are upregulated in cancer which are likely to be acting as oncogenes and those downregulated in cancer which are likely to be acting as tumour suppressors. (ukessays.com)
  • Both function as tumor suppressors, which means they keep cells from growing and dividing too rapidly or in an uncontrolled way. (medlineplus.gov)
  • Without one of these tumor suppressors, cells can grow and divide unchecked, leading to the development of cancer. (medlineplus.gov)
  • Twelve miRNAs showed concordant expression in tumors vs. normal breast tissues and patient survival (n = 1093), with seven as potential tumor suppressors and five as potential oncomiRs. (cdc.gov)
  • [ 1 ] Early stage head and neck squamous cell carcinoma (HNSCC) is treated relatively well with single-modality therapy (either surgery or radiation alone). (medscape.com)
  • Go to Imaging of Nasopharyngeal and Laryngeal Squamous Cell Carcinoma and Head and Neck Squamous Cell Carcinoma for complete information on these topics. (medscape.com)
  • Oral squamous cell carcinoma (OSCC) is a common tumor in the world. (degruyter.com)
  • Targeting constitutive and interleukin-6-inducible signal transducers and activators of transcription 3 pathway in head and neck squamous cell carcinoma cells by curcumin (diferuloylmethane). (greenmedinfo.com)
  • Constitutive activation of STAT3 in head and neck squamous cell carcinoma (HNSCC) and its role in proliferation of this tumor has been demonstrated. (greenmedinfo.com)
  • Novel neoadjuvant therapy regimens are warranted for oral squamous cell carcinoma (OSCC). (bvsalud.org)
  • PURPOSE: Our previous studies have found that Stathmin, a microtubule depolymerizing protein, is a potential biomarker to guide locally advanced oral squamous cell carcinoma (OSCC) induction chemotherapy. (bvsalud.org)
  • This brain tumor type is highly proliferative and shows an infiltrative growth pattern, accounting for the high recurrence rates in patients. (hindawi.com)
  • The PromoCell 3D Tumorsphere Media XF ( C-28070 , C-28075 ) is designed to serially passage brain tumor derived cell lines as undifferentiated 3D neurospheres with high cell proliferation rates. (sigmaaldrich.com)
  • Glioblastoma multiforme is the most common and lethal primary human brain tumor. (unina.it)
  • The applicant is responsible for a brain tumor tissue biobank which provides the basis for the studies on human GBM tumor cells and stroma. (lu.se)
  • Distinct hallmarks of cancer were examined using in silico analysis, various in vitro cell culture assays, and ex vivo organotypic rat brain slice culture model. (hindawi.com)
  • We investigated the impact on tumor malignancy in F98 glioma cells using in silico analysis, in vitro cell culture assays, and ex vivo vascular organotypic glioma impact model (VOGIM) [ 35 ] by implanting F98 cells into rat brain slices. (hindawi.com)
  • Transwell migration, cell cycle analysis, MTT and colony formation assays were performed to determine the effect of DRAM2 overexpression and knockdown on NSCLC-cell migration and proliferation. (cancerindex.org)
  • Relative cell viability was assessed using MTT assays. (wjgnet.com)
  • Programmed cell death assays commonly used in mammalian systems such as DNA laddering or TUNEL assays, are often difficult to reproduce in plants. (jove.com)
  • To test this hypothesis we examined the dose-dependent cytotoxicity effects of single-walled (SWCNT) and multi-walled (MWCNT) carbon nanotube s on NHBE and A549 cells using lactate dehydrogenase (LDH) release and the cell proliferation/viability WST-1 assays. (cdc.gov)
  • Combined with in-vitro proliferation assays using CRISPR-Cas9/RNAi and patient survival analysis, MEK inhibitors PD19830 and BRD-K12244279, pilocarpine, and tremorine were discovered as potential new drug options for treating breast cancer. (cdc.gov)
  • Rapamycin could fully inhibit S6 in all cell lines, but this was accompanied by activation of Akt phosphorylation. (aacrjournals.org)
  • Background: The phosphorylation of NF-kappaB inhibitor alpha (IκBα) protein is pivotal to the regulation of NF-κB transcription factor activity in the cell. (researchsquare.com)
  • We investigated the new phosphorylation site for IκBα and identified its biological function in breast cancer cells. (researchsquare.com)
  • The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. (cancerindex.org)
  • Numerous reports suggest that interleukin-6 (IL-6) promotes survival and proliferation of tumor cells through the phosphorylation of a cell-signaling protein, signal-transducer-and-activator-of-transcription-3 (STAT3). (greenmedinfo.com)
  • In the present report, we demonstrate that most HNSCC cell lines had constitutively active STAT3 and that curcumin (diferuloylmethane), a pharmacologically safe agent in humans, inhibited STAT3 phosphorylation in a dose- and time-dependent manner. (greenmedinfo.com)
  • When OSCC cells with GDF15 overexpression were treated with the erbB2 phosphorylation inhibitor, CI-1033, cell proliferation and xenograft growth colony formation were significantly blocked (P (bvsalud.org)
  • Consistently, among 13 drugs targeting chromatin modifiers, EHMT2 inhibitors were highly effective in reducing ERMS cell viability. (elifesciences.org)
  • AKT is a key molecule in the PI3K/AKT/mTOR signaling pathway, which has been shown to serve notable functions in the regulation of cell viability and to be closely associated with a variety of disorders caused by dysfunctional cellular proliferation ( 8 , 9 ). (spandidos-publications.com)
  • iP300w reduces viability and inhibits proliferation of CIC-DUX4 sarcoma (CDS) cells in vitro but not pancreatic or colorectal cancer cell lines. (tocris.com)
  • The combination of NV651 and cisplatin, a DNA damage reagent, resulted in a synergistic effect in cell viability, and a significant increase on cell death in HCC cell lines. (lu.se)
  • Here we investigated the characteristics of a population of pancreatic cancer tumorspheres derived from different human pancreatic cancer cell lines and a primary line from a genetically engineered KPC mouse model, using flow cytometry and western blotting to analyse surface and stemness markers. (lu.se)
  • Finally, we provide protocols for analyzing these cells through flow cytometry and immunocytochemistry. (lu.se)
  • Herein, we sought to determine if rapamycin, an inhibitor of mTOR, could enhance erlotinib sensitivity for cell lines derived from a variety of tissue types (non-small-cell lung, pancreatic, colon, and breast). (aacrjournals.org)
  • In highly heterogeneous tumours, like pancreatic ductal adenocarcinoma (PDAC) however, the characterisation of cancer stem cells has led to controversial results due to the lack of consensus on specific markers. (lu.se)
  • Suppresses tumor growth and improves survival in mice bearing pancreatic tumor xenografts. (tocris.com)
  • Pancreatic cancer is a highly invasive malignant tumor of the digestive system. (jcancer.org)
  • Pancreatic cancer is a highly invasive type of malignant tumor with a very poor prognosis [ 1 ] . (jcancer.org)
  • A human Pancreatic duct epithelial cells line (HPDE), HEK-293T and three human pancreatic cancer cell lines (CanPan-1, Hs-766T, CFPAC-1) were obtained from Chinese typical culture preservation center. (jcancer.org)
  • Akt also regulates the mammalian target of rapamycin (mTOR)-S6K-S6 pathway to control cell growth in response to growth factors and nutrients. (aacrjournals.org)
  • IRS-1 integrates signalling from insulin receptor (InsR), insulin-like growth factor-1 receptor (IGF1R) and many other cytokine receptors and is elevated in β-catenin induced cells. (wikipedia.org)
  • The HER (erbB) family of transmembrane receptor tyrosine kinases is one of the cytostatic targets in tumor cell growth and survival. (medscape.com)
  • However, there has only been one previous functional study investigating MCT4 in glioblastoma (GBM) neurospheres, mainly focusing on tumor growth and survival dependent on the oxygen level [ 14 ]. (hindawi.com)
  • The reduced tumor growth upon EHMT2 deficiency was reversed by recombinant DKK1 or LGK974, which also inhibits Wnt signaling. (elifesciences.org)
  • Along with the suppression and cessation of growth of cancer cells, numerous important cells are also damaged, stressed, or destroyed. (bigthink.com)
  • In a report in Developmental Cell, the scientists say their advance could lead to strategies for controlling both normal growth and the spread of cancer, processes that depend on the programmed, organized movement of cells across space. (news-medical.net)
  • Platelet-derived growth factor receptor β (PDGFRβ), a cell-surface tyrosine kinase receptor, is an important hallmark involved in glioma since it influences several cellular processes of tumor biology including proliferation, migration, and angiogenesis. (unina.it)
  • As a result, the combination of Gint4.T and an epidermal growth factor receptor-targeted aptamer is better at slowing tumor growth than either single aptamer alone. (unina.it)
  • The compound suppresses tumor growth in a mouse CDS xenograft model. (tocris.com)
  • Because tumor growth is essentially exponential, with one cell dividing into two, two to four, four to eight, and so on, a tumor cell product such as PSA can reflect such exponential growth-measuring the time it takes for PSA to double (PSA doubling time, or PSADT). (lifeextension.com)
  • Without p16(INK4A) to regulate cell growth and division (proliferation), cells can continue to grow and divide without control, which can lead to tumor formation. (medlineplus.gov)
  • Somatic mutations in other genes involved in cell growth are also needed for a melanoma to develop. (medlineplus.gov)
  • Together, the germline and somatic mutations impair the function of proteins that regulate division and senescence, leading to uncontrolled cell growth and the formation of a melanoma. (medlineplus.gov)
  • CDKN2A gene mutations involved in cancer impair production of functional p16(INK4A) or, less commonly, p14(ARF), which can result in uncontrolled cell growth and tumor formation. (medlineplus.gov)
  • Clotrimazole is a broad-spectrum antifungal agent that inhibits yeast growth by altering cell membrane permeability, causing death of fungal cells. (medscape.com)
  • In addition, NV651 decreased tumour growth in xenograft-mouse model. (lu.se)
  • Furthermore, overexpression of SOX11 in EOC cell lines confirmed the growth regulatory role of SOX11. (lu.se)
  • In this study, we found that the SCARA5 expression was lower in CAL-27 and SCC-9 cells than that in human normal oral epithelial keratinocytes. (degruyter.com)
  • In addition, SCARA5 repressed OSCC cell epithelial-mesenchymal transformation (EMT), evidenced by increased E-cadherin expression and reduced N-cadherin expression. (degruyter.com)
  • Comparison of carbon nanotube -induced cytotoxicity in A549 and normal human bronchial epithelial (NHBE) cells. (cdc.gov)
  • Pulmonary bronchial epithelial cells are a potential target for toxicity during respiratory exposure. (cdc.gov)
  • The tumor cell line A549, a pulmonary human type-II like epithelial cells, is widely used for evaluation of particle toxicity. (cdc.gov)
  • We hypothesized that A549 cells are more resistant to toxicity compared to the normal epithelial cells. (cdc.gov)
  • Overall, the results presented here indicate that SWCNT or MWCNT induce cytotoxicity in human lung epithelial cells in a time and dose-dependent manner. (cdc.gov)
  • Furthermore, we demonstrate a large variation between the particle-induced cytotoxic responses in A549 cells and normal primary epithelial cells. (cdc.gov)
  • Background: The neural transcription factor SOX11 has been described as a prognostic marker in epithelial ovarian cancers (EOC), however its role in individual histological subtypes and tumour grade requires further clarification. (lu.se)
  • Breast cancers are most often epithelial tumors involving the ducts or lobules. (msdmanuals.com)
  • General references Breast cancers are most often epithelial tumors involving the ducts or lobules. (msdmanuals.com)
  • In order to induce its effects on the intestinal epithelial cells, LTD4 binds to its receptor CysLT1R. (lu.se)
  • Monocarboxylate transporter 4 (MCT4, SLC16A3 ) is elevated under hypoxic conditions in many malignant tumors including gliomas. (hindawi.com)
  • Slowly growing metastases to bone, liver, lymph nodes, and lung can arise from malignant tumors. (medscape.com)
  • These data are then used to identify both genetically distinct subtypes of sarcoma and molecular signatures associated with tumor morphology, grade, recurrence, and survival. (mskcc.org)
  • One major research line is to clarify changes in the glioblastoma (GBM) stroma between primary surgery and tumor recurrence. (lu.se)
  • Biomarkers for identification of these histologically identical WHO II tumors do not exist. (nature.com)
  • Exploratory endpoints are the relationships between PD-L1 combined positive score (CPS, defined as the number of PD-L1-stained cells divided by the total number of viable tumour cells, multiplied by 100) and other immunological and genomic biomarkers and response. (bvsalud.org)
  • Multi-omics biomarkers of response to the discovered drugs were identified using human breast cancer cell lines. (cdc.gov)
  • In this study, we aimed to evaluate the possible effects of tumor-stromal interactions on local estrogen biosynthesis in endometrial cancer. (scirp.org)
  • We isolated stromal cells from endometrial cancer patients to examine local biosynthesis of estrogens and tumor-stromal interactions. (scirp.org)
  • Next we examined the effects of steroid sulfatase inhibitor and aromatase inhibitor in monoculture of endometrial cancer cell line (Ishikawa) and in a co-culture system involving an Ishikawa cells and stromal cells. (scirp.org)
  • This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. (cancerindex.org)
  • Tissue from both primary surgery and reoperations from the same patients, allows in depth analysis of the evolution of glioblastoma over time with the goal of development of diagnostic monitoring and identification of druggable GBM stromal targets in recurrent brain tumors. (lu.se)
  • We have identified and isolated mesenchymal stromal cells and studied some of their functions. (lu.se)
  • The purpose of the present study was to reveal the effects of AKT1 on the survival and proliferation of HCC cells, and to investigate the mechanisms involved. (spandidos-publications.com)
  • This task can be accomplished by replacing tumor suppressor genes that have been lost or mutated, selectively inserting genes that produce cytotoxic substances, or modifying the immune system to destroy the tumor cells. (medscape.com)
  • 1) The alternation of protein levels of critical oncogenes or tumour suppressor genes, which causes by miRNAs deregulation may also lead to proliferative diseases such as cancer. (ukessays.com)
  • Here we show that methanolic extracts of Sapota fruit (MESF) induces cytotoxicity in a dose-dependent manner in cancer cell lines. (nature.com)
  • As our understanding of the molecular biology of HNSCC continues to develop, we can target the specific components of cancer cells that are not found in normal cells. (medscape.com)
  • BMC Cell Biology, 9 , 45. (springer.com)
  • He also has professional interests in stem cell research, the biology of aging, cancer as well as telomere and telomerase biology. (stanford.edu)
  • BT-474 cells, obtained expression of the receptor has important implications for their biology from American Type Culture Collection, were maintained in RPMI 1640 and therapy (1). (lu.se)
  • Microarrays were prepared in tumor biology. (lu.se)
  • However, its proliferation was significantly decreased by the treatment of steroid sulfatase or aromatase inhibitors. (scirp.org)
  • We evaluated the effects of STS inhibitor and aromatase inhibitors on the proliferation of estrogen-dependent endometrial cancer cells. (scirp.org)
  • It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. (joplink.net)
  • Here we show that TGF-beta regulates proliferation, migration, and tumorigenicity of mesenchymal GBM cancer stem cells (CSCs) in vivo and in vitro. (uni-regensburg.de)
  • The mechanism behind malignancy and tumor genesis is unknown, limiting development of alternative therapies. (nature.com)
  • In summary, our data highlight MCT4 /SLC16A3 as a key gene for distinct hallmarks of tumor malignancy in glioma cells. (hindawi.com)
  • Pheochromocytomas and catecholamine-secreting paragangliomas have a similar clinical presentation, but the risk for associated neoplasms, risk for malignancy, and genetic testing is different between the tumors. (medscape.com)
  • Targeted molecular therapy, like therapy with monoclonal antibodies, gene therapy, and other therapies, has limited or nonexistent side effects on normal cells of the body, unlike present modalities such as surgery, chemotherapy, and radiotherapy. (medscape.com)
  • Chemoresistance, or the insensitivity of cancer cells to drug therapy, is a major factor in the failure of chemotherapy against breast cancer. (hindawi.com)
  • BCSCs can develop into many cell types and repopulate heterogeneous tumors following conventional chemotherapy or radiotherapy [ 4 , 6 ]. (hindawi.com)
  • While there are many applications and complications associated with chemotherapy, the commonly understood mechanism is the paralyzing of bone marrow, which leads to lower amounts of red and white blood cells and platelets. (bigthink.com)
  • Published in the journal Nature Communications , researchers at Northwestern University have discovered a genetic "kill code" that could enable the destruction of cancer cells without the "spray and pray" mentality of chemotherapy. (bigthink.com)
  • The small RNA molecules can effectively kill cancer cells, a process that chemotherapy is meant to activate. (bigthink.com)
  • They found that chemotherapy drugs kill cancer cells in part by triggering the release of a toxic mechanism, resulting in the release of tumor-suppressive miRNAs. (bigthink.com)
  • Erlotinib could inhibit extracellular signal-regulated kinase, Akt, and S6 only in cell lines that were the most sensitive. (aacrjournals.org)
  • The identified potential therapeutic targets of HNK (PTTH) included CCND1 , SIRT2 , AURKB , VEGFA , HDAC1 , CASP9 , HSP90AA1 , and HSP90AB1 , which can potentially inhibit the cell cycle of mBCSCs. (hindawi.com)
  • He has also examined the cooperativity of Withaferin A with other interventions that are known to inhibit gliomas such as combination therapies of Withaferin A with temozolomide, with other ginsenosides and with alternating electric fields (known as tumor treating fields or TTFields). (stanford.edu)
  • Loss of DKK3 gene is associated with a possible tumor suppressor role in human cancers. (amrita.edu)
  • In very aggressive cancers, bone marrow stem cells are obliterated and must be replaced. (bigthink.com)
  • In 2012, he became a member of the Canary Center at Stanford University where he has leveraged his experience in cell and preclinical studies to develop imaging modalities to track therapeutic responses against cancer as well as detecting early stage cancers. (stanford.edu)
  • The new steroidal analogues 5, 6, 8, 9, 11 and 12 exhibited moderate cytotoxic effects in sixty cancer cell lines derived from nine cancers types. (strath.ac.uk)
  • Compared with the normal tissues, the expression of MicroRNA-16-5precursor was low in glioma tissues, which was consistent with that in the Uppsala87 and Uppsala251 cell lines. (ijpsonline.com)
  • Cyclophilin overexpression is a common event in fibrotic tissues playing a key role in different stages of the fibrotic process, including inflammation, hepatocyte death, and activation of the hepatic stellate cells leading to increased collagen production. (lu.se)
  • Tissues and Cells. (lu.se)
  • As loss of RPL5/RPL11 abrogates ribosome biogenesis and protein synthesis to the same extent as loss of other essential 60S RPs, we reasoned the loss of RPL5 and RPL11 would induce a p53-independent cell cycle checkpoint. (nih.gov)
  • Moreover, we dissected the signaling pathway from the cell surface receptors to RhoA using a broad-spectrum G protein coupled receptor (GPCR) antagonist, [D-Arg1,D-Trp5,7,9,Leu11]Substance P (SP), and a recently reported Galphaq/11-selective inhibitor, YM-254890. (nih.gov)
  • Most of the important molecular processes in the cell, such as DNA replication, are performed by large molecular network of protein-protein interactions. (researchsquare.com)
  • The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. (cancerindex.org)
  • The intersection between the differentially expressed genes (DEGs) and the HNK-targets was determined using a Venn diagram, and the results were analyzed using a protein-protein interaction network, hub gene selection, gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses, genetic alteration analysis, survival rate, and immune cell infiltration levels. (hindawi.com)
  • Sarcoma tissue samples and cell lines are subjected to a multiplatform genome-wide characterization of expression of protein-coding genes and microRNAs, DNA copy number changes, activating mutations, gene rearrangements and methylation profiling. (mskcc.org)
  • 1) The regulation of these protein levels is vital for the understanding of the cell cycle control and its dysregulation. (ukessays.com)
  • 2) Therefore, the proper control of protein levels is crucial for the cell cycle. (ukessays.com)
  • Reporting in the journal Biochemical and Biophysical Research Communications (BBRC), bioengineering researchers at UC San Diego published videos of a key message-carrying protein called paxillin moving abruptly from hubs of communication and transportation activity on the cell surface toward the nucleus. (news-medical.net)
  • Primary tumors can spread towards distant organs as single cells, when they lose a protein called E-cadherin. (news-medical.net)
  • 1 Prostate-specific antigen is a protein produced by the cells of the prostate gland, including both cancerous cells as well as cells that are benign. (lifeextension.com)
  • Further analyses of EOC cell lines showed that SOX11 mRNA and protein were expressed in two of five cell lines, correlating with promoter methylation status. (lu.se)
  • Demethylation was successfully performed using 5'-Aza-2'deoxycytidine (5-Aza-dC) resulting in SOX11 mRNA and protein expression in a previously negative EOC cell line. (lu.se)
  • In this study, we examined both the expression levels and the activation states of RhoA in various lung cancer cells by quantitative real-time reverse transcriptase-polymerase chain reaction and in vivo Rho guanine nucleotide exchange assay, respectively. (nih.gov)
  • We found that four of six small cell lung carcinoma (SCLC) cell lines exhibited a moderate to high activation rate of RhoA. (nih.gov)
  • Our results suggest that RhoA is activated in various lung cancer cells independent of its expression levels, and the high activation state of RhoA in SCLC cells mainly depends on a neuroendocrine peptide autocrine system which signals through Galpha12 coupled GPCR to RhoA. (nih.gov)
  • This study provides new insights into RhoA signaling in lung cancer cells and may help in developing novel therapeutic strategies against lung cancer. (nih.gov)
  • DRAM2 acts as an oncogene in non-small cell lung cancer and suppresses the expression of p53. (cancerindex.org)
  • Here, we show that DRAM2 may act as an oncogenic regulator in non-small cell lung cancer (NSCLC). (cancerindex.org)
  • membranous co-expression of CADM1 and LATS2 in lung tumors predicts good prognosis. (cancerindex.org)
  • Cell adhesion molecule-1 (CADM1) is a member of the immunoglobulin superfamily that functions as a tumor suppressor of lung tumors. (cancerindex.org)
  • Curcumin was also a more potent inhibitor of HNSCC cell proliferation than AG490. (greenmedinfo.com)
  • Treatment with the novel cyclophilin inhibitor NV651, presented a potent antiproliferative effect in HCC cell lines via cell cycle perturbations arresting cells in the mitotic phase. (lu.se)
  • In this paper, the mechanism of MicroRNA-16-5p in the occurrence and development of glioma was studied by the cell experiment in vitro. (ijpsonline.com)
  • This mechanism may be at least partially responsible for curcumin's ability to suppress proliferation of HNSCC cells. (greenmedinfo.com)
  • 2 If PSA readings begin to elevate, there are interventions that can reduce or stabilize the production of PSA, shutting down a mechanism used by cancer cells to escape their confinement within the prostate gland. (lifeextension.com)
  • AKAP12 knockdown in benign meningioma cells SF4433 increases proliferation, cell cycle, migration, invasion, and confers an anaplastic profile. (nature.com)
  • DRAM2 overexpression also increased proteins CDK4, CyclinD3, and decreased p27 expression, all of which are cell cycle-related factors. (cancerindex.org)
  • BCSCs are mostly dormant, and therefore, activating them and modulating the cell cycle are important for successful therapy against BCSCs. (hindawi.com)
  • However, the molecular mechanisms of action of HNK in BCSCs, as well as its effects on the cell cycle, remain unclear. (hindawi.com)
  • This study aimed to explore the potential targets and molecular mechanisms of HNK on metastatic BCSC (mBCSC)-cell cycle arrest and the impact of the TME. (hindawi.com)
  • Finally, the interaction between HNK and two HNK-targets regulating the cell cycle was analyzed using molecular docking analysis. (hindawi.com)
  • These results strongly circumscribe the possible mechanisms of PINK1 action in the mitochondrial life cycle and also raise the possibility that mitochondrial turnover events that occur in cultured embryonic axons might be restricted to the cell body in vivo, in the intact nervous system. (sdbonline.org)
  • 10) Therefore, it is important to evaluate its role in the cell cycle and its dysregulation. (ukessays.com)
  • These proteins help regulate the cell cycle, which is the cell's way of replicating itself in an organized, step-by-step fashion. (medlineplus.gov)
  • CDK4 and CDK6 normally stimulate the cell to continue through the cycle and divide. (medlineplus.gov)
  • This combination caused a disturbance in the cell cycle and a decrease in the capacity of the cell to repair interstrand crosslinks. (lu.se)
  • Targeting the cell cycle has been proposed as a therapeutic strategy in cancer treatment with special interest in the mitotic phase. (lu.se)
  • A robust in vitro cell culture system supporting HBV and HDV infections (HBV/HDV) is a critical prerequisite to studying HBV/HDV pathogenesis, the complete life cycle of HBV/HDV infections, and consequently identifying new therapeutics. (cdc.gov)
  • In a preliminary study it has been shown that phenolic antioxidants such as methyl 4- O -galloylchlorogenate and 4- O -galloylchlorogenic acid derived from Sapota fruits can induce cytotoxicity in colon cancer cells 23 . (nature.com)
  • sis factor alpha (TNF-), tumor necrosis factor beta (TNF- ), interleukin (IL)-1, and IL-2 (11-14). (cdc.gov)
  • We have already acquired proof-of-principle showing that reprogramming towards astrocytic fate causes morphological changes, reduces proliferation and bestow astrocytic function in human GBM cell lines. (lu.se)
  • Moreover, MCT4 overexpression enhances cell migration and invasiveness via reorganization of the actin cytoskeleton. (hindawi.com)
  • Pote ntly inhibits tumor cell proliferation in a variety of tumor cell lines in vitro and in vivo. (chemspider.com)
  • The results indicated that OSCC cells overexpressing GDF15 were sensitive to TPF through a caspase-9-dependent pathway both in vitro and in vivo. (bvsalud.org)
  • As a third major line of research, we explore, in collaboration with associate professor Henrik Ahlénius group if GBM cells can be reprogrammed to neuron like cells in vitro and in vivo. (lu.se)
  • In vivo, a substantially increased infiltration of immune cells was observed in mesenchymal GBMs, while immune infiltrates were rare in proneural GBMs. (uni-regensburg.de)
  • After cloning the antibody genes into an expression vector, this is then transfected into an appropriate host cell line for antibody expression. (cellsignal.com)
  • Total RNA from tumors the expression of thousands of genes (3) to address complex questions was isolated using two successive rounds of Trizol. (lu.se)
  • Six miRNAs had concordant expression in both tumors and breast cancer patient blood samples compared with the normal control samples. (cdc.gov)
  • Although intraductal papillomas (IDPs) are benign, they are occasionally involved by a monomorphic, atypical cellular proliferation, morphologically identical to ductal carcinoma in situ (DCIS) or atypical ductal hyperplasia (ADH). (medscape.com)
  • Some diagnose atypical papilloma (also known as papilloma with atypia) if the monomorphic cellular proliferation is smaller than 3 mm in greatest dimension and is low grade, and diagnose DCIS involving a papilloma if the proliferation is 3 mm or larger or is intermediate to high grade. (medscape.com)
  • [ 1 , 2 ] Others advocate the diagnosis of DCIS involving a papilloma irrespective of the size and grade of the atypical cellular proliferation. (medscape.com)
  • Large/central IDP subtypes (L/C ST) may be involved by complex, crowded benign cellular proliferations involved by varying degrees of apocrine metaplasia, which can mimic the histologic features of DCIS (see Microscopic Findings ). (medscape.com)
  • Several studies have shown IDPs involved by suspicious cellular proliferations seen on CNB are more commonly associated with cancer upon excision than IDPs without atypia diagnosed on CNB (see Prognosis and Predictive Factors ). (medscape.com)
  • It changes the permeability of the fungal cell membrane after binding to cell membrane sterols, causing cellular contents to leak. (medscape.com)
  • However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
  • Breast cancer stem cells (BCSCs) are one of the main factors driving chemoresistance, thereby contributing to poor prognosis and clinical outcomes [ 3 - 5 ]. (hindawi.com)
  • It's defined and serum-free formualtion allows consistent and standardized expansion of neurospheres while maintaining the cancer stem cell characteristics such as self-renewal and chemoresistance. (sigmaaldrich.com)
  • Cell proliferation rates of U-87 MG glioblastoma cells during 3D neurosphere culture. (sigmaaldrich.com)
  • Neurosphere proliferation rates of U-87 MG glioblastoma cells ( 89081402 ) were higher over 11 passages in Promocell's 3D Tumosphere Media XF vs. other competitior neurosphere culture media. (sigmaaldrich.com)
  • This process enables tumor cells, including those within the core of the heterogeneous tumor mass to survive, despite being at a distance from blood vessels that supply their metabolic needs [ 16 , 17 ]. (hindawi.com)
  • Estrogen receptor and steroid sulfatase mRNA levels in cancer cells were significantly higher in the co-cultures compared with the monocultures of endometrial cancer cells. (scirp.org)
  • Estradiol and androstenediol concentrations were also significantly higher in the co-cultured cells. (scirp.org)
  • Proliferation of the cancer cells was significantly increased through the steroid sulfatase pathway, which metabolizes androgens, estrone sulfate, and estradiol sulfate as its substrates. (scirp.org)
  • On a functional level, proneural CSC lines caused a significantly stronger TGF-beta-dependent suppression of NKG2D expression on CD8(+) T and NK cells in vitro providing a mechanistic explanation for the reduced immune infiltration of proneural GBMs. (uni-regensburg.de)
  • Cell proliferation, q-PCR, Western blot, subcutaneous xenograft and other experimental methods were used to value the regulatory effect of vincristine on Stathmin. (bvsalud.org)
  • Methods: Previously we observed that aurora kinase (AURK) binds IκBα in the cell. (researchsquare.com)
  • METHODS: Stathmin overexpressed and knockdown stable cell lines were constructed. (bvsalud.org)
  • Stable F98 glioma cell lines with MCT4 overexpression, normal expression, and knockdown were generated. (hindawi.com)
  • We found that DKK3 expression levels were low in GBC patients and cell lines. (amrita.edu)
  • Tumor specimens from 259 NSCLC patients were collected and analyzed. (cancerindex.org)
  • With the development of tumor molecular heterogeneity theory(12), there are still some patients with GIST who are sensitive to radiotherapy, especially for the patients with advanced stage(13-16).In addition, with the development of imaging technology and modern tissue and organ radiation technology, it has become a reality to concentrate high-dose radiation locally in abdominal cavity tumors(12), which challenges RT's insensitivity to GISTs. (researchsquare.com)
  • Patients achieving MPR show more CD4+ T-cell infiltration than those without MPR (P = 0.02), and decreased CD31 and É -SMA expression levels are observed after neoadjuvant therapy. (bvsalud.org)
  • Early diagnosis is important because the tumor may be fatal if undiagnosed, especially in pregnant women during delivery or in patients undergoing surgery for other disorders. (medscape.com)
  • We analyzed 19 acute-phase serum sam- cells, with subsequent release of high systemic levels of ples for mitogenic activity from patients with severe strepto- cytokines (12-15). (cdc.gov)
  • The serum samples from two patients in Several lines of evidence support the hypothesis of SAg the acute phase of STSS showed strong proliferative activ- ity. (cdc.gov)
  • simultaneously bind to major histocompatibility complex published a study of seven patients with severe streptococ- class II molecules and T-cell receptor molecules bearing a cal infections: SPE-A was detected in serum samples from four patients (27). (cdc.gov)
  • 2014). genetic StudieS are carried out, uSing tuMour SaMPleS froM PatientS with excellent clinical data that have been collected at a PoPulation level or internationally, to Provide unique data coMbining the Pathology, geneticS, clinical featureS, and ePideMiology of tuMourS. (who.int)
  • Several academic and industry efforts are well under owned subsidiary Cyto Therapeutics, it had received way to produce dopaminergic neurons from stem approval by the Australian government to conduct a cells under conditions compliant with use in patients. (lu.se)
  • A second press release [2] has indicated stem cell source, resulting in widespread excitement that the program is planning to move forward very rapidly, with all of the patients being enrolled in the Correspondence to: Roger A. Barker, John van Geest Centre first quarter of 2016 and interim results being shared for Brain Repair, Department of Clinical Neurosciences, Univer- in October 2016. (lu.se)
  • Malignant gliomas are the most common primary malignant brain tumors with an increasing incidence of up to nine per 100,000 habitants over the last years [ 1 , 2 ]. (hindawi.com)
  • 2006. Tumor stem cells derived from glioblastomas cultured in bFGF and EGF more closely mirror the phenotype and genotype of primary tumors than do serum-cultured cell lines. (sigmaaldrich.com)
  • The primary endpoints are safety and major pathological response (MPR, defined as ≤10% residual viable tumour cells). (bvsalud.org)
  • Recently, the development of the HepG2-NTCP sec+ cell line, expressing the sodium taurocholate co-transporting polypeptide receptor (NTCP) and self-assembling co-cultured primary human hepatocytes (SACC-PHHs) has opened new perspectives for a better understanding of HBV and HDV lifecycles and the development of specific antiviral drug targets against HBV/HDV infections. (cdc.gov)
  • In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (cdc.gov)
  • Changes in dietary fatty acids, specifically the polyunsaturated fatty acids of the ω-3 and ω-6 families and some derived eicosanoids from lipoxygenases, cyclooxygenases, and cytochrome P-450, seem to control the activity of transcription factor families involved in cancer cell proliferation or cell death. (springer.com)
  • Curcumin inhibits head and neck cancer cell proliferation. (greenmedinfo.com)
  • Testing this method on four cell lines, two human and two mouse, the team identified over 700 targets leading to cancer cell proliferation. (bigthink.com)
  • We therefore chose to study CysLT1 in colon cancer specimens and in non-transformed and colon cancer cell lines. (lu.se)
  • Resolving the Pathogenesis of Anaplastic Wilms Tumors through Spatial Mapping of Cancer Cell Evolution. (lu.se)
  • Besides inhibiting constitutive expression, curcumin also abrogated the IL-6-induced activation of STAT3 in HNSCC cells. (greenmedinfo.com)
  • Mutations in the CDKN2A gene are found in up to one-quarter of head and neck squamous cell carcinomas (HNSCC). (medlineplus.gov)
  • The goal of specific molecular targets in cancer therapy is to create a "magic bullet" that selectively kills cancer cells. (medscape.com)
  • Ideal targets should be both specific to cancer cells and commonly found in cancer cells. (medscape.com)
  • In vitro cell culture systems are instrumental in the development of therapeutic targets. (cdc.gov)
  • Cell culture systems contribute to identifying molecular mechanisms for HBV and HDV propagation, finding drug targets for antiviral therapies, and testing antiviral agents. (cdc.gov)
  • We found that RhoA was expressed highly in large cell carcinoma cells but only weakly in adenocarcinoma cells. (nih.gov)
  • We observed low expression of DKK3 in gallbladder adenocarcinoma tumors and highly invasive GBC cell lines. (amrita.edu)