Proliferation tumor stem cell assay. Medical search. Frequent questions

FDA approved drugs not currently used to treat ependymoma were also identified that posses selective toxicity against ependymoma cells relative to normal NSCs both in vitro and in vivo, e.g., 5-fluorouracil. (nih.gov)
Memory cell proliferation (activation) can also be assayed in vitro with CFSE chemical tagging. (wikipedia.org)
In comparison with GD2 - A549 cells, GD2 + A549 cells showed significantly enhanced cell proliferation ( P ＜0.05),invasion ( P ＜0.05) and migration ability ( P ＜0.01) in vitro . (magtechjournal.com)
Three human GC cell lines (AGS, NCI-N87, HGC-27) were selected for in vitro experimental validation. (bvsalud.org)
As a result, monoclonal antibody 8B6 immunotherapy significantly increased temozolomide genotoxicity and tumor cell death in vitro by enhancing temozolomide tumor uptake. (stemcellsciencenews.com)
In both the in vitro and the in vivo models, ganciclovir therapy targeting proliferating GSCs promoted the survival of a quiescent, stem-like cell pool capable of reproducing the tumor upon release of the therapeutic pressure. (stemcellsciencenews.com)
DMH-1 is a selective inhibitor of bone morphogenic protein (BMP) type-I receptor activin receptor-like kinase 2 (ALK2) receptor (IC 50 = 108 nM or 12.6 nM in in vitro kinase assays). (tocris.com)
DMH-1 suppresses lung cancer cell proliferation, migration, invasion in vitro and reduces tumor growth in a mouse lung cancer xenograft model. (tocris.com)
This study utilizes proliferation assays, clonogenic assays, death assays and soft agar colony formation assays using murine pancreatic acinar cancer cells and immortalized normal human pancreatic acinar cells to demonstrate ST6GAL1 influencing stem-like cell behaviors in vitro. (uab.edu)
In-vitro assays including cell viability, colony formation, AC + 5-FU drug combination index and tumor sphere generation were applied to determine the inhibitory effect of AC. (tmu.edu.tw)
NK cells cytokine expression, signaling and cytotoxicity has been evaluated in vitro. (bmj.com)
Conclusions The current report clearly demonstrates that ADSCs can enhance different malignant properties of DFSP cells in vitro, which should not be neglected when considering the clinical use of human ADSCs and its related derivatives in skin regenerative therapies. (archive.org)
Zhaoqi Yuan, Zhu Zhu, Fangxing Zhu, Feixue Ding, Yinmin Wang, Xiuxia Wang, Xusong Luo, Jun Yang, Fei Liu, Di Sun. 'Impact of human adipose tissue-derived stem cells on dermatofibrosarcoma protuberans cells in an indirect co-culture: an in vitro study. (archive.org)
The aim of this study was to determinate the effect of different chemotherapy protocols used in the clinic on tumor stem cell markers in vitro . (isciii.es)
More importantly, deficient-autophagy decreased the protein expression of stemness markers and caused the disappearance of the superiority in tumorigenicity in vitro and vivo in CD271+ OS cells. (biomedcentral.com)
Because cells in vivo interact with their environment in three dimensions, 3D cell culture tools, reagents, and techniques have led to the creation of more predictive in vitro cell models for diverse applications and disciplines including cancer research, drug discovery, neuroscience, and regenerative medicine. (sigmaaldrich.com)
In preparation to apply various toxicants to the developed in vitro human neuronal stem cells, the team has characterized a number of PCB's and PAH compounds for their ability to (a) induce various signal transduction mechanisms and (b) inhibit gap junctional intercellular communication regulating cell proliferation, differentiation, and apoptosis. (nih.gov)
Tetrazolium reduction and resazurin assays are the mainstay of routine in vitro toxicity batteries. (cdc.gov)

Enhancer of zeste homolog 2 (EZH2) belongs to poly-sulfur protein family and can mediate cell proliferation and differentiation via the modulation of various genes expressions. (medscimonit.com)
Global transcriptome and chromatin occupancy analyses of ZNF148 revealed a central role in inhibiting cancer cell de-differentiation and migration. (nature.com)
Tumor initiation and progression often involve the dysregulation of developmentally important genes in cancer cells, endowing them with stem cell-like features such as enhanced self-renewal, invasiveness, and an aberrant differentiation state [ 1 ]. (nature.com)
The MYC proto-oncogene is a transcription factor essential in stem/progenitor cell maintenance and differentiation [ 11 ]. (nature.com)
Developmentally, it is well established that expression of the ID members is strongest in stem/progenitor cells, while their levels decrease upon differentiation. (nature.com)
CD44 is a multistructural and multifunctional cell surface molecule involved in cell proliferation, cell differentiation, cell migration, angiogenesis, presentation of cytokines, chemokines, and growth factors to the corresponding receptors, and docking of proteases at the cell membrane, as well as in signaling for cell survival. (wikipedia.org)
Additionally, RAD18 functions beyond DNA repair and may regulate many other key biological processes, such as chromatin strengthening, cell survival/death, stemness, and differentiation. (nature.com)
Cell viability and differentiation capacity toward the mesenchymal lineages were assessed, along with immunophenotype. (biomedcentral.com)
In this position , you will be part of a team (ERC-PoC, NWO Demonstrator) to characterize several lead compounds using cell-based assays measuring the potency and specificity of Notch/g-secretase inhibitors and the effects of Notch activity, proliferation, differentiation, and self-renewal of normal and tumor tissues. (academictransfer.com)
Mammalian adult stem cells resemble the blastomeres of planktonic and benthic organisms with small eggs and may have evolved in mature organisms as an adaptation to the growth and maintenance of tissues via proliferation and the regulation of organ size via cell loss (e.g., terminal differentiation). (iospress.com)
Krüppel-like factors can bind to specific DNA motifs and regulate various cellular functions, such as metabolism, cell proliferation, and differentiation. (medsci.org)
KLFs regulate a wide range of cellular functions, including cell proliferation, apoptosis, differentiation, and neoplastic transformation, by binding to GC-rich promoter regions [ 1 , 2 ]. (medsci.org)
DMH-1 induces intestinal differentiation in human intestinal organoids (hIOs) derived from human pluripotent stems cells (hPSCs). (tocris.com)
We have explored the regulation of signalling pathways by p63 and BRCA1 that are involved in growth control, differentiation and stem cell regulation. (biomedcentral.com)
This may result from the presence of cancer stem cells (CSCs), which form a small population in tumors with metastatic potential, high invasive capacity, self-renewal, and differentiation. (fapesp.br)
Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells. (sigmaaldrich.com)
CD27 and its ligand CD70 are involved in multiple aspects of T cell biology, including the costimulation and expansion of CD4+ and CD8+ T cells and differentiation to memory CD8+ T cells. (stemcell.com)
The expansion of activated B cells and their differentiation to plasma cells is also reported to be enhanced by CD27. (stemcell.com)

Using a mouse model of ependymoma-a chemoresistant brain tumor-we combined multicell high-throughput screening (HTS), kinome-wide binding assays, and in vivo efficacy studies, to identify potential treatments with predicted toxicity against neural stem cells (NSC). (nih.gov)
In the context of brain tumor initiating cells (BTICs), PDX models allow for characterization of tumor formation, growth, and recurrence, in a clinically relevant in vivo system. (mcmaster.ca)
Ex vivo glycan engineering of the surface of live cells has been used to enforce HCELL expression on any cell that expresses CD44. (wikipedia.org)
The results in vivo indicated that tumors formed by GD2 + A549 cells grew faster ( P ＜0.001) and were more malignant ( P ＜0.01) as compared to GD2 - A549 cells. (magtechjournal.com)
In vivo experiments were verified by establishing a subcutaneous transplantation tumor model in nude mice, observing the size and volume of tumors in vivo in nude mice, and obtaining fresh tissues for subsequent experiments. (bvsalud.org)
Prior to their clinical application, hASCs must be expanded ex vivo to obtain the required number of cells for transplantation. (biomedcentral.com)
Karyotype analysis was performed and tumorigenesis assay in vivo was also evaluated. (biomedcentral.com)
Moreover, the hASCs presented normal karyotype undergoing senescence, and did not form in vivo tumors, eliminating the possibility that spontaneous immortalization of hASCs had occurred with pooled allogeneic human serum. (biomedcentral.com)
In addition, you will be part of the studies determining the in vivo efficacy and safety of new compounds using preclinical tumor models and the development of a companion biomarker. (academictransfer.com)
We are looking for a post-doc researcher with a proven track record in Molecular Biology or Biochemistry and an interest in drug development, stem cell biology, and in vivo models. (academictransfer.com)
To demonstrate cell behaviors in vivo, pancreatitis mouse model with overexpressing ST6GAL1 was created, and immunofluorescent staining was done on pancreas tissue samples. (uab.edu)
One was ex vivo and one in vivo and both illustrated the inhibition characteristics vitamin C has on new tumor blood vessel growth. (prnewswire.com)
The in vivo assay treated with vitamin C indicated 30% less blood vessel growth than untreated tissue. (prnewswire.com)
Of course, in the body, that's not how it behaves at all-tumors are in 3D masses…and a drug that looks good on plastic doesn't necessarily mean that it will be good in vivo. (genengnews.com)
Cells are embedded in hydrogels to simulate the in vivo extracellular matrix. (sigmaaldrich.com)
Though these manufactured supports are less like the in vivo ECM, they may enhance reproducibility and facilitate cell retrieval from the culture. (sigmaaldrich.com)
In vivo experiments were conducted using a co-implantation xenograft mouse model with live cell imaging and immunohistochemistry. (cdc.gov)

In addition, it is further related with occurrence and metastasis of malignant tumors. (medscimonit.com)
CD44 participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. (wikipedia.org)
Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. (wikipedia.org)
Splice variants of CD44 on colon cancer cells display sialofucosylated HCELL glycoforms that serve as P-, L-, and E-selectin ligands and fibrin, but not fibrinogen, receptors under hemodynamic flow conditions pertinent to the process of cancer metastasis. (wikipedia.org)
Cancer stem-like cells (CSCs) are associated with cancer progression, metastasis, and recurrence, and may also represent a subset of circulating tumor cells (CTCs). (biorxiv.org)
Combined, these data indicate that CD117 expression potentially promotes tumor initiation and metastasis. (biorxiv.org)
Cancer stem cells, instrumental in metastasis, would seem to ignore mechanisms normally functioning in the removal of excess cells. (iospress.com)
Our study is the first to provide demonstrate that KLF6 functions as a tumor suppressor gene and prevents the metastasis of oral cancer cells. (medsci.org)
Researchers demonstrated that exposure to anti-CDH11 antibody suppressed metastasis, reduced CDH11, fibronectin and β-catenin expression, and abrogated the CSC-like traits of metastatic breast cancer (MBC) cells. (stemcellsciencenews.com)
Using intravenous injection of B16F10 melanoma cell line and EO711 triple negative breast cancer cell line, metastasis evaluation was performed. (bmj.com)
Cish fl/fl Ncr1 Ki/+ NK cells display lower activation thresholds and Cish fl/fl Ncr1 Ki/+ mice are more resistant to tumor metastasis and to primary breast cancer growth. (bmj.com)
The term TICs is now more widely used in cancer biology, often referring to or overlapping with cellular origin of cancer, most recent common ancestor of cancer (based on sequencing data), cancer cells with stem cell-like properties, therapy-resistant cancer cells, or metastasis-initiating cells. (mdpi.com)

GBM stem-like cells (GSCs) present in tumors are felt to be highly tumorigenic and responsible for tumor recurrence. (oncotarget.com)
The loss of KLF6 expression is also correlated with cancer progression, tumor recurrence, and short survival time in head and neck carcinomas [ 7 ]. (medsci.org)
Cancer stem cells (CSC's) are considered as one of the key contributors in the development of drug resistance and tumor recurrence. (tmu.edu.tw)
Accumulating evidence shows that cancer stem cells are key drivers of tumor formation, progression, and recurrence. (hindawi.com)
Cancer stem cells are responsible for tumor chemoresistance and recurrence in adjuvant and metastatic settings. (isciii.es)
Recurrence and chemoresistance in pancreatic cancer are largely related to cancer stem cells. (isciii.es)
OS cancer stem cells (CSCs) also are considered a key factor of tumorigenesis, drug resistance and tumor recurrence. (biomedcentral.com)

ADAR1 silencing in breast cancer cells leads to increased apoptosis, suggesting an anti-apoptotic function that promotes cancer progression. (cancerindex.org)
The MYC proto-oncogene ( MYC ) is one of the most frequently overexpressed genes in breast cancer that drives cancer stem cell-like traits, resulting in aggressive disease progression and poor prognosis. (nature.com)
GBM stem-like cells (GSCs) represent a subpopulation of the tumor which is responsible for tumor initiation, progression, and re-growth after chemoradiation [ 5 , 6 ], as well as supporting tumor vessel growth and function [ 7 ]. (oncotarget.com)
CD44 variant isoforms are also relevant to the progression of head and neck squamous cell carcinoma. (wikipedia.org)
This editorial highlights the relevance of interfering in cancer cell progression through the pharmacological manipulation on the cell metabolism of cyclic nucleotides such as cAMP, and on the intracellular Ca2+ signaling, which may avail the reduction of toxic effects promoted by chemotherapy, radiotherapy and immunotherapy, thus decreasing the incidence of interruption in antitumoral treatment. (researchgate.net)
Further, in cell lines, CD117 activation by SCF promotes faster proliferation and invasiveness, while blocking CD117 activation with tyrosine kinase inhibitors (TKIs) decreased progression in a context-dependent manner. (biorxiv.org)
Our previous study showed that it plays an essential role in the progression of multiple tumors. (nature.com)
Tumor microenvironment (TME) remodeling is a critical process in primary tumor progression. (nature.com)
This study successfully uncovers cell behaviors that promote the transition from ADM to neoplasia, offering novel avenues to inhibit PDAC progression. (uab.edu)
Second, Dr. Cho's group discovered that two of the type I Melanoma Antigen Genes (MAGE), MAGE-A3 and MAGE-C1, are commonly expressed in multiple myeloma and are correlated with progression of disease and proliferation. (themmrf.org)
Efforts to link CD44 to CSCs and tumor progression should consider the expression of various CD44 isoforms. (lu.se)
Mechanistically, an upregulation of PDL-1 in chemotherapy-treated CAFs led to an increase in hepatocyte growth factor (HGF) secretion, which stimulates cancer progression, cell invasion, and stemness of lung cancer cells, while inhibiting apoptosis. (cdc.gov)

These findings uncover a previously unknown tumor suppressor role for ZNF148, and a transcriptional regulatory circuitry encompassing MYC, ZNF148, and ID1/3 in driving cancer stem cell traits in aggressive breast cancer. (nature.com)
KLF6 functions as a tumor suppressor gene and increases p21 expression via p53-independent pathway [ 3 ]. (medsci.org)
S100A2, a tumour suppressor, is a known p63 target. (biomedcentral.com)
Mechanistically, AC mediated anti-tumor effect was associated with an increased level of tumor suppressor microRNAs especially, miR142-3p. (tmu.edu.tw)
They demonstrated that type I MAGE plays a critical role in conferring resistance to chemotherapy and inhibition of apoptosis in myeloma cells through regulation of Bcl-2 family proteins and the tumor suppressor p53. (themmrf.org)

Knockdown of KDM1B inhibits cell proliferation and induces apoptosis of pancreatic cancer cells. (cancerindex.org)
Although KLF6 overexpression in human cancer cells inhibits cell proliferation, induces apoptosis, and attenuates cell migration, the effects of KLF6 on oral cancer remains poorly elucidated. (medsci.org)
however, forced KLF6 expression in lung cancer cell lines can trigger cells to undergo apoptosis and reduce colony formation ability [ 6 ]. (medsci.org)
A successful therapy must therefore eliminate these cells known to be highly resistant to apoptosis. (hindawi.com)
In this paper, we describe the metabolic changes as well as the mechanisms of resistance to apoptosis occurring in cancer cells and cancer stem cells, underlying the connection between these two processes. (hindawi.com)
Outcomes We showed that KLF9 appearance coupled with HDAC inhibitor panobinostat (LBH589) significantly induced glioma stem cell loss of life via both apoptosis and necroptosis within a synergistic way. (immune-source.com)
Furthermore, we showed that necrosis and apoptosis inhibitors conferred minimal defensive results against cell loss of life, while inhibitors from the necroptosis pathway blocked cell loss of life significantly. (immune-source.com)
Conclusions Our results suggest an in depth knowledge of how KLF9 appearance in cancers cells with epigenetic modulators like HDAC inhibitors may promote synergistic cell loss of life through a system regarding both apoptosis and necroptosis which will benefit book combinatory antitumor ways of treat malignant human brain tumors. (immune-source.com)
Role of PDL-1 in NSCLC chemoresistance was assessed using CRISPR/Cas9 knockdown and various functional assays including MTT, cell invasion, sphere formation, and cell apoptosis. (cdc.gov)

However, the mechanism through which RAD18 influences triple-negative breast cancer (TNBC), especially the interaction between tumor cells and the tumor microenvironment, remains elusive. (nature.com)
Within the lab, four PI-led teams focus on identifying actionable dependencies in tumor cells and the tumor microenvironment that can be targeted to enhance and prolong treatment response while preserving normal tissue function. (academictransfer.com)
microenvironment of DFSP cells. (archive.org)
First, they are investigating immunologic therapies with a focus on using cutting edge genomic and immunologic assays to map the interaction between myeloma and the immune system in the tumor microenvironment. (themmrf.org)
In contrast, cancer cells shift their metabolism toward lactate production even in the presence of oxygen [ 4 ], partly through genetic modifications that stabilize the transcription factor Hypoxia Inducible Factor (HIF) involved in the adaptation of the cells to hypoxia, under nonhypoxic conditions as well as generating an adaptive response to the hypoxic microenvironment (Figure 1 ). (hindawi.com)
The theoretical basis for marrow failure includes primary defects in or damage to the stem cell or the marrow microenvironment. (medscape.com)

In breast cancer research CD44+/CD24- expression is commonly used as a marker for breast CSCs and is used to sort breast cancer cells into a population enriched in cells with stem-like characteristics and has been seen as an indicator of increased survival time in epithelial ovarian cancer patients. (wikipedia.org)
Results CSCs of A549 cell were successfully enriched by and the percentage of GD2 + cells was 0.57% in adherent cell, but the percentage of GD2 + cells was able to increase to 20.4% after spheroid culturing. (magtechjournal.com)
This observation has led to the 'cancer stem cell' (CSCs) hypothesis, stating that a subpopulation of cancer cells survive therapy and lead to tumor relapse. (mdpi.com)
Tumor-sphere formation and cell viability assay revealed a greater inhibition of CSC proliferation and antineoplastic activity of IL-32γ in CD133 + CSCs as compared with normal cancer cells. (stemcellsciencenews.com)
Investigators established long-term culturable patient-derived cancer cells (PDCs) from patient endometrial tumors by spheroid cell culture, which is favorable for the enrichment of CSCs. (stemcellsciencenews.com)
CD271+ OS CSCs and CD271- OS cells were isolated by magnetic activated cell sorting. (biomedcentral.com)
Our data indicated that CD271+ OS CSCs had a similar basic autophagy level with CD271- OS cells. (biomedcentral.com)
The results above demonstrated that autophagy contributes to the stem-like features of CD271+ OS CSCs. (biomedcentral.com)
Cancer stem cells (CSCs) are seen as a subpopulation of self-renewing tumor cells, which can differentiate into the daughter tumor cells, have the lower sensitivity to chemotherapy and radiotherapy, and exhibit tumor re-initiating property. (biomedcentral.com)

Using xenograft limiting dilution assays and serial tumor initiation assays, we show that CD117+ cells represent a CSC population. (biorxiv.org)
The oncogenicity of different subpopulations in cell line A549 was compared with tumor xenograft model. (magtechjournal.com)
PDC-derived xenograft tumors were generated in immunodeficient NOG mice. (stemcellsciencenews.com)
Morphologically, PDCs derived from three distinct patient samples and their xenograft tumors recapitulated the corresponding original patient tumors. (stemcellsciencenews.com)

Glioma tumor tissues were collected from 3 patients who received surgery, and the glioma stem cells were then separated, cultured, and identified by flow cytometry. (medscimonit.com)
Here we investigated the characteristics of a population of pancreatic cancer tumorspheres derived from different human pancreatic cancer cell lines and a primary line from a genetically engineered KPC mouse model, using flow cytometry and western blotting to analyse surface and stemness markers. (lu.se)
Flow cytometry was utilized to determine the stem cell marker CD44 expression status in GCSCs. (bvsalud.org)
For this purpose, both SP and non-SP cells were sorted out by flow cytometry as indicated by trapezoids around the left (R5) and right (R8), respectively (Fig. 2a), and seeded into 96 well plates at 500 cells/well. (nanoker-society.org)

This study investigated the effect of EZH2 expression on proliferation and tumorigenesis of brain glioma cells. (medscimonit.com)
The effect on tumorigenesis potency of glioma stem cells was determined by mouse transplantation assay. (medscimonit.com)
Our data demonstrated that in brain glioma cells, the decrease of EZH2 level could suppress cell proliferation and tumorigenesis potency, and meanwhile inhibit the expressions of oncogenes including c-myc and Akt. (medscimonit.com)
Ganglioside GD2 identifies breast cancer stem cells and promotes tumorigenesis[J]. J Clin Invest, 2012, 122: 2066-2078. (magtechjournal.com)
The Notch signaling pathway is an evolutionary conserved signal transduction cascade present in almost all tissues and is required for embryonic and postnatal development, as well as for stem cell maintenance, but it is also implicated in tumorigenesis including pancreatic cancer and leukemia. (uni-ulm.de)

Treatment selection takes into account patient age, fitness, and whether autologous stem cell transplantation (ASCT) is planned. (medscape.com)
This program has a special focus on immunologic therapy in the setting of autologous stem cell transplantation for myeloma. (themmrf.org)
The concept of TICs is originally a functional definition, largely based on transplantation assays. (mdpi.com)
The specific medications administered depend on the choice of therapy and whether it is supportive care only, immunosuppressive therapy, or hematopoietic cell transplantation. (medscape.com)
Central venous catheter placement is required before the administration of hematopoietic cell transplantation. (medscape.com)
High-dose melphalan followed by autologous peripheral blood stem cell transplantation may also be used. (msdmanuals.com)
The essence of MDS is damage of In the current work we examined All our patients were of the high-risk colony-forming units [4], but the defect haematopoietic stem cells of high-risk group and none of them was eligible of the haematopoietic stem cells is not MDS cases for apoptotic and anti-apop- for stem cell transplantation. (who.int)

ZNF148 suppressed cell proliferation and migration and was transcriptionally repressed by MYC in breast cancer. (nature.com)
Depletion of ZNF148 by short hairpin RNA (shRNA) and CRISPR/Cas9 increased triple-negative breast cancer (TNBC) cell proliferation and migration. (nature.com)
This study aimed to investigate the relationship between miR-506 and proliferation and migration of breast cancer cells. (medscimonit.com)
Cell proliferation, cell counting, colony formation assay, and Transwell assay were applied to evaluate the proliferation and migration of breast cancer cells. (medscimonit.com)
Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. (cancerindex.org)
The CD44 antigen is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. (wikipedia.org)
HCELL functions as a "bone homing receptor", directing migration of human hematopoietic stem cells and mesenchymal stem cells to bone marrow. (wikipedia.org)
We demonstrate that CD117+ cells display increased proliferation and migration. (biorxiv.org)
KLF6 overexpression attenuated the migration and invasion of oral cancer SAS cells. (medsci.org)
However, RBPJL can bind to canonical RBPJ DNA elements and shows migration dynamics comparable to that of RBPJ in the nuclei of living cells. (uni-ulm.de)
studies indicated that RANTES is Purpureaside C an adipokine that can be produced by adipocytes and takes on an important part in T cell migration, suggesting a potential part of the RANTES/CCR5 axis in adipose T cell build up in obesity (24). (exposed-skin-care.net)

Cancer stem cells are a population of slow-cycling cells within the tumour bulk, with self-renewal capacity that attracts interest as a therapeutic target. (lu.se)
Tumor-Initiating Cells (TICs), also known as Cancer Stem Cells, refer to a subset of cancer cells capable of self-renewal proliferation and producing all other cancer cell types within a tumor. (mdpi.com)

The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
The E3 ubiquitin ligase RAD18 is well known for the maintenance of genome stability and cell survival through many DNA damage response (DDR) pathways such as translesion DNA synthesis (TLS) and homologous recombination repair (HRR) [ 4 , 5 ]. (nature.com)
Likewise, strategies for the containment of cancer might be based on promoting normal pathways of cell loss, the basal mode for handling excess cells. (iospress.com)
Global gene expression of Cish fl/fl Ncr1 Ki/+ NK cells compared with Cish +/+ Ncr1 Ki/+ NK cells revealed upregulation of pathways and genes associated with NK cell cycling and activation. (bmj.com)
Lipids, amino acids, and nucleotides necessary for the biosynthesis of the daughter cells are mostly provided by intermediate metabolites of these pathways. (hindawi.com)
To prevent aberrant cell proliferation, these pathways are tightly regulated. (hindawi.com)
This study suggests that breast cancer stem-like cells could be selectively inhibited by targeting signaling pathways important for breast cancer stem-like cells. (nanoker-society.org)
Cancer cells are characterized by the procurement of several capacities amid the stages of tumor genesis, maintenance of proliferation signaling, induction of angiogenesis, activation of invasive resistance to cell death, inhibition of growth suppressors, and metastatic pathways, ability to evade immunological destruction and alteration of cellular metabolism. (gastroconferences.com)

To demonstrate the inhibitory effect of bufalin on glioma cells and glioma stem-like cells (GSCs) and discuss the underlying mechanism, the proliferation of glioma cells was detected by MTT and colony formation assays following treatment with bufalin. (techscience.com)

In tumor angiogenesis, the blood vessels grow to support the growth of the tumor. (prnewswire.com)
This groundbreaking BCRI study has shown that angiogenesis or the proliferation of new blood vessels, in support of tumor growth, is retarded when high levels of vitamin C are present in the blood. (prnewswire.com)
In the study published in the February 2010 issue of Journal of Angiogenesis Researc h, two assays were used to evaluate the effect of high-doses of vitamin C on the inhibition of new blood vessel growth. (prnewswire.com)

miR-506 over-expression may thus be able to improve the malignant phenotype of breast cancer cells. (medscimonit.com)
High expression of RAD18 facilitated a highly stem-cell phenotype through the Hippo/YAP pathway, which supports the proliferation of TNBC. (nature.com)
In addition, the cytokine byproduct TGF-β activates macrophages to have an M2-like tumor-associated macrophage (TAM) phenotype. (nature.com)
Due to this, the sialic acid changes the structure and function of cell surface receptor proteins and regulates signal transduction and consequently, the cell phenotype. (uab.edu)
Moreover, given that this enzyme is upregulated in cancer and our preliminary data shows that ST6GAL1 drives the cell towards a more Acinar to Ductal Metaplasia-like phenotype, I hypothesize that expression of ST6GAL1 promotes stem-like cell behaviors, such as enhanced survival and invasiveness, thereby promoting neoplasia. (uab.edu)
Overall, the results showed that overexpression of ST6GAL1 provides the cells with a greater proliferative, pro-survival and invasive phenotype. (uab.edu)
Infiltration and phenotype of NK cells in the tumor was evaluated. (bmj.com)
Tumors characterized as positive for the CD44+/CD24- phenotype by immunohistochemistry were associated to all isoforms except the CD44 standard (CD44S) isoform, which lacks all variant exons. (lu.se)

Inhibition of YAP or TGF-β breaks this loop and suppresses cancer stemness and proliferation In nude mice, RAD18 promoted subcutaneous transplanted tumor growth and M2-type TAM recruitment. (nature.com)
Tumorigenicity ability was evaluated by colony and sphere formation assay, the protein expression of stemness markers and tumor formation in nude mice. (biomedcentral.com)

CD44 has been referred to as HCAM (homing cell adhesion molecule), Pgp-1 (phagocytic glycoprotein-1), Hermes antigen, lymphocyte homing receptor, ECM-III, and HUTCH-1. (wikipedia.org)
CD44 is expressed in a large number of mammalian cell types. (wikipedia.org)
One critical modification involves discrete sialofucosylations rendering the selectin-binding glycoform of CD44 called HCELL (for Hematopoietic Cell E-selectin/L-selectin Ligand). (wikipedia.org)
CD44 gene transcription is at least in part activated by beta-catenin and Wnt signalling (also linked to tumour development). (wikipedia.org)
CD44, along with CD25, is used to track early T cell development in the thymus. (wikipedia.org)
CD44 expression is an indicative marker for effector-memory T-cells. (wikipedia.org)
In addition, variations in CD44 are reported as cell surface markers for some breast and prostate cancer stem cells. (wikipedia.org)
Endometrial cells in women with endometriosis demonstrate increased expression of splice variants of CD44, and increased adherence to peritoneal cells. (wikipedia.org)
High levels of the adhesion molecule CD44 on leukemic cells are essential to generate leukemia. (wikipedia.org)
Furthermore, because alternative splicing and posttranslational modifications generate many different CD44 sequences, including, perhaps, tumor-specific sequences, the production of anti-CD44 tumor-specific agents may be a realistic therapeutic approach. (wikipedia.org)
The existence of cancer stem cells (CSC) expressing CD44 and a high aldehyde dehydrogenase (ALDH) activity has recently been demonstrated in gastric carcinoma and has opened new perspectives to develop targeted therapy. (bmrat.org)
After analyzing different tumor stem cell markers (SOX2, OCT4, CD133, CD44 and CD24) in pancreatic cancer cells treated with different chemotherapeutic protocols by means of RT-qPCR, Oxaliplatin and Gemcitabine in monotherapy were the chemotherapies that selected the most cancer stem cells while the FOLFIRI protocol decreased them. (isciii.es)
Tras analizar mediante RT-qPCR diferentes marcadores de células madre tumorales (SOX2, OCT4, CD133, CD44 y CD24) en células de cáncer de páncreas tratadas con diferentes protocolos quimioterapéuticos, el Oxaliplatino y la Gemcitabina en monoterapia fueron los quimioterápicos que seleccionaron en mayor medida las células madre cancerosas mientras que el protocolo FOLFIRI las disminuyó. (isciii.es)
Subpopulations positive for CD44, CD133, and CD117 or lacking these biomarkers were classified as laryngeal cancer stem cells (LCSCs) or laryngeal cancer non-stem cells (non-LCSCs), respectively. (fapesp.br)
Implications: These results suggest that pro-proliferative and pro-stemness effects of Reg4 are mediated through g-secretase-mediated CD44/CD44ICD signaling, hence strategies to disrupt Reg4-CD44-g-secretase-CD44ICD signaling axis may increase cancer cell susceptibility to chemo- and radiotherapeutics. (wustl.edu)
CD44 isoforms are heterogeneously expressed in breast cancer and correlate with tumor subtypes and cancer stem cell markers. (lu.se)
The CD44 cell adhesion molecule is aberrantly expressed in many breast tumors and has been implicated in the metastatic process as well as in the putative cancer stem cell (CSC) compartment. (lu.se)
We used q-RT-PCR and exon-exon spanning assays to analyze the expression of four alternatively spliced CD44 isoforms as well as the total expression of CD44 in 187 breast tumors and 13 cell lines. (lu.se)
Breast cancer cell lines showed a heterogeneous expression pattern of the CD44 isoforms, which shifted considerably when cells were grown as mammospheres. (lu.se)
Unsupervised hierarchical cluster analysis of CD44 isoform expression data divided tumors into four main clusters, which showed significant correlations to molecular subtypes and differences in 10-year overall survival. (lu.se)

Methods Primary DFSP cells were indirectly co-cultured with ADSCs in a conditioned medium or in a Transwell system. (archive.org)

Multiple myeloma is a cancer of plasma cells that produce monoclonal immunoglobulin and invade and destroy adjacent bone tissue. (msdmanuals.com)
Rarely, patients have no M-protein in blood and urine, although the currently used serum free light chain assay now demonstrates monoclonal light chains in many of these formerly so-called nonsecretory patients. (msdmanuals.com)

CDK18 knockdown or ATR inhibition in glioblastoma stem-like cells (GSCs) suppressed homologous recombination and conferred PARP inhibitor (PARPi) sensitivity, with ATR inhibitors synergizing with PARPis or sensitizing GSCs. (stemcellsciencenews.com)
The inhibitory effects of IL-32γ on tumor development were associated with inhibition of the STAT5 pathway. (stemcellsciencenews.com)
As shown in Darusentan Darusentan Fig. 1, PTL, PDTC and DETC at 1 M inhibited MCF7 sphere cell growth by 33.2, 50.8 and 52.2%, respectively, but did not show obvious growth inhibition effect on MCF7 bulk cells. (nanoker-society.org)
Interestingly, unlike cancer drug paclitaxel (2.5 nM), which inhibited MCF7 SP cells by 39.3%, and non-SP cells by 52.2%, all the three compounds showed preferential inhibition for the MCF7 SP cells over the non-SP cells (Fig. 2b). (nanoker-society.org)
Synergistic inhibition of GSC viability by KLF9 appearance and HDAC inhibitors We additional examined whether concurrent KLF9 manifestation alongside additional HDAC inhibitors, i.e. vorinostat (SAHA) or trichostatin (TSA), enhanced cell death in GSCs. (immune-source.com)

We identified kinases within the insulin signaling pathway and centrosome cycle as regulators of ependymoma cell proliferation, and their corresponding inhibitors as potential therapies. (nih.gov)

Then, orthotopic implantation of breast tumors was performed and tumor growth was followed using bioluminescence. (bmj.com)

Matrigel invasion and colony forming assays were performed to confirm CSC presence. (fapesp.br)

and (iii) Reg4 significantly increases colorectal cancer and pancreatic cancer cell proliferation and their clonogenic potential in stem cell assays. (wustl.edu)
The clonogenic capacity of stem cells was evaluated by the stem cell colony formation assay. (gastroconferences.com)

TAMs are the major inflammatory cells in the TME, and are closely related to poor outcomes in several types of tumors [ 11 ]. (nature.com)

To determine how CD117 expression and activation by its ligand stem cell factor (SCF, kit ligand, steel factor) alter prostate cancer aggressiveness, we used LNCaP-C4-2 and PC3-mm human prostate cancer cells, which contain a CD117+ subpopulation. (biorxiv.org)
In addition, expression of TrkB, KRAS, HIF-1 alpha, and VEGF-A genes and proteins related to cell proliferation were evaluated in this subpopulation. (fapesp.br)
We found that the LCSC subpopulation demonstrated more resistance to cetuximab and paclitaxel combination chemotherapy when compared with the non-LCSC subpopulation of the cell line. (fapesp.br)

This hypothesis predominantly focuses on the transition between normal pancreatic acinar to tumor cell and the importance of ST6GAL1 in the tumor initiation process on which few studies have been reported. (uab.edu)

ABSTRACT This study examined haematopoietic stem cells of 19 high-risk cases of myelodysplastic syndrome (MDS) for apoptotic and anti-apoptotic signals and cellular proliferation and correlated these with clinical and cytogenetic subtypes, particularly trisomy 8. (who.int)

Disialoganglioside GD2 expression in solid tumors and role as a target for cancer therapy[J]. Front Oncol, 2020, 10. (magtechjournal.com)
Conclusion This study represents a crucial step in the mechanistic understanding and safety of Cish targeting to unleash NK cell antitumor function in solid tumors. (bmj.com)

Using molecular modeling, biochemical and functional assays, as well as single-molecule time-lapse imaging, we show that RBPJL and RBPJ, despite limited sequence homology, possess a high degree of structural similarity. (uni-ulm.de)

A sphere formation assay was performed to evaluate the sphere-forming capabilities of GCSCs. (bvsalud.org)

Immunohistochemical staining revealed that nuclear KLF6 level was significantly and inversely associated with tumor size and stages. (medsci.org)
AC treatment significantly inhibited the proliferation, colony formation and tumor sphere generation. (tmu.edu.tw)
MCF7 cells were shown to contain 1.2% SP cells, which could significantly be blocked by ABC transporter inhibitor verapamil (0.12%) (Fig. 2a). (nanoker-society.org)
High-risk MDS cases had a significantly higher percentage of apoptotic CD34+ cells and anti-apoptotic survivin+ cells than controls, particularly for trisomy 8 cases. (who.int)

Disialogangliosides GD2 and GD3 are involved in the attachment of human melanoma and neuroblastoma cells to extracellular matrix proteins[J]. J Cell Biol, 1986, 102: 688-696. (magtechjournal.com)
CD27 interacts with TRAF family members, resulting in enhanced cellular proliferation and the induction of anti-apoptotic proteins. (stemcell.com)

Taken together, our data demonstrated that bufalin inhibits glioma cell growth and proliferation, inhibits GSC proliferation, and kills GSCs. (techscience.com)
Effects of cadmium on root growth, cell division and micronuclei formation in root tip cells of Allium cepa var. (techscience.com)
Recent advances and new insights in the management of early-stage epidermal growth factor receptor-mutated non-small-cell lung cancer[J]. World J Clin Oncol, 2021, 12: 912-925. (magtechjournal.com)
Anti-GD2 antibody dinutuximab inhibits triple-negative breast tumor growth by targeting GD2(+) breast cancer stem-like cells[J]. J Immunother Cancer, 2021, 9. (magtechjournal.com)
Their larval and adult cells have narrow potencies, sometimes coupled to virtually unlimited … proliferation, and function in the growth, maintenance and regulation of body size. (iospress.com)
The embryos of larger arthropods and deuterostomes with well-provisioned eggs or viviparity, on the other hand, exhibit regulative development, while their larval "set-aside" or adult stem cells function in the growth, maintenance, and regulation of organ size coupled to constrained proliferation and cell turnover. (iospress.com)
Strategies for regenerative therapies in adult mammals, therefore, might be based on stimulating growth of adult stem cells or their surrogates in specific tissues rather than on introducing embryonic stem cells into adults. (iospress.com)
WICHITA, Kan. , Feb. 26 /PRNewswire/ -- Bio-Communications Research Institute's (BCRI's) recent research study has found that high levels of vitamin C (ascorbate) inhibit the formation of new blood vessel growth to tumors. (prnewswire.com)
This nutrient flow is critical to tumor growth and is facilitated in the host body by the growth of new blood vessels. (prnewswire.com)
PTL (5 M) inhibited MCF7 SP cell growth by 95.2% and non-SP cells by 66.1% (Fig. 2b). (nanoker-society.org)
This study is focused on identifying the cell surface binding partner of Reg4 and dissecting its role in colorectal cancer and pancreatic cancer growth and stem cell survival. (wustl.edu)
External insults (eg, infections, radiation, drugs) may disrupt stem cell homeostasis in marrow environment, leading to altered growth. (medscape.com)

Cultures are subjected to a standard 2D viability assay to determine the health of the cells two days after treatment with the test compound. (genengnews.com)

The use of hASCs for therapeutic applications has grown substantially in the last years, because the use of stem cells from adult tissues circumvent some ethical issues associated with the application of embryonic stem cells, and because of their accessibility via isolation from lipoaspirates, a disposable byproduct of cosmetic surgery. (biomedcentral.com)
When cells are not grown on supports, they may form 3D aggregates called spheroids, which secrete their own ECM to become more like native solid tissues. (sigmaaldrich.com)
Liver cancer is the condition which occurs when normal cells in the liver become abnormal in appearance and destructive to the adjacent normal tissues, which can spread to other areas of liver or to the other organs outside of the liver. (gastroconferences.com)
In obese humans, adipocyte-secreted CCL20 may contribute to the deposition of Compact disc4+ helper and Compact disc8+ cytotoxic T lymphocytes within adipose tissues, possibly via connections with CCR6 which was upregulated on T cells in obese adipose tissues (100). (exposed-skin-care.net)
However, the main element substances that mediate T cell infiltration into adipose tissues in maturing remain to become discovered. (exposed-skin-care.net)
Activation of Typical T Cells in Adipose Tissues Compact disc4+ Purpureaside C T Cell Activation TCRs recognize the current presence of a particular antigen by binding to brief peptide sequences in the antigen that's shown on APCs. (exposed-skin-care.net)
further defined that mostly huge adipocytes from obese adipose tissues exhibited an increased expression degree of MHCII substances and acted as APCs to activate Compact disc4+ T cells to secrete IFN- (103). (exposed-skin-care.net)

The authors studied the overlap between metastatic stem cells and tumor-initiating cells in the MMTV-PyMT model. (stemcellsciencenews.com)
They showed that in the MMTV-PyMT model, Lin - CD90 - ALDH high cells retained a high tumor-initiating potential in orthotopic transplants, in contrast to Lin - CD24 + CD90 + , which retained higher metastatic capacity. (stemcellsciencenews.com)

ATR inhibitor VE822 combined with PARPi extended survival of mice bearing GSC-derived orthotopic tumors, irrespective of PARPi-sensitivity. (stemcellsciencenews.com)
Results In Cish fl/fl Ncr1 Ki/+ mice, we detected no developmental or homeostatic difference in NK cells. (bmj.com)
CD27 is found on peripheral T cells, memory B cells, and NK cells and in mice has been found on most thymocytes, except CD46-CD8- precursors. (stemcell.com)
Another statement showed the preadipocyte- and endothelial cell-derived stromal-derived element-1 (CXCL12), mediated early infiltration of CD4+ T lymphocytes in obesity, which preceded the increase of macrophages in adipose cells of mice on HFD (101). (exposed-skin-care.net)
Indeed, the principal adipocytes isolated from obese mice could induce antigen-specific Compact disc4+ T cell activation (58). (exposed-skin-care.net)
indicated that ATMs colocalized with T cells in lymphoid clusters within adipose tissue and may act as APCs, which express high levels of MHCII and also costimulatory molecules and process and present antigens to induce CD4+ T-cell proliferation and activation in adipose tissue of obese mice (29, 68, 105). (exposed-skin-care.net)
showed that adipose tissue from obese mice induced proliferation of splenic CD8+ T cells, indicating a CD8+ T cell-activating environment in obese adipose tissue (31). (exposed-skin-care.net)

[ 2 ] Options for second-line therapy in patients with relapsed/refractory disease include chemotherapy-free regimens with biologic targeted agents such as covalent Bruton tyrosine kinase (BTK) inhibitors, lenalidomide,venetoclax, and chimeric antigen receptor (CAR) T-cell therapy. (medscape.com)
Results PTL, PDTC and DETC preferentially inhibit MCF7 sphere cell proliferation It has been shown that NF-B pathway specific inhibitors, including MG-132, PTL and PDTC could selectively inhibit leukemia stem cell proliferation [25C27]. (nanoker-society.org)
Among all the inhibitors, antioxidants which inhibit NF-B activation including PDTC and its analog DETC, and NF-B phosphorylation inhibitors PTL were shown to preferentially inhibit sphere cell proliferation. (nanoker-society.org)
It was of interest to test whether the various NF-B inhibitors that are preferentially active against sphere cells as above have similar effect on MCF7 SP cells. (nanoker-society.org)
Methods Since GBM neurosphere cultures from patient-derived gliomas are enriched for GBM stem-like cells (GSCs) and form highly intrusive and proliferative xenografts that recapitulate the features showed in human sufferers identified as having GBM, we set up inducible KLF9 appearance systems in these GBM neurosphere cells and looked into cell loss of life in the current presence of epigenetic modulators such as for example histone deacetylase (HDAC) inhibitors. (immune-source.com)
This sensation is additional corroborated with the upregulation of cell routine inhibitors p21 and p27. (immune-source.com)
MTS assays indicated related loss in cell viability in KLF9-expressing GSCs when treated with SAHA or TSA (Fig.?2a, b), suggesting a common tumor cell killing effect of KLF9 in conjunction with HDAC inhibitors. (immune-source.com)

In contrast to normal cells, most cancer cells predominantly produce energy by a high rate of glycolysis followed by lactate fermentation, even in the presence of oxygen, a less efficient metabolism compared to a low rate of glycolysis followed by mitochondrial oxidation of pyruvate [ 2 ]. (hindawi.com)
These results demonstrate differential sensitivity of standard cytotoxicity assessments on the PPP, thus (1) decoupling 'mitochondrial activity' as an interpretation of cellular formazan and Alamar Blue metabolism, and (2) demonstrating the implicit requirement for investigators to sufficiently verify interaction of these methods in routine cytotoxicity and proliferation characterization. (cdc.gov)

In highly heterogeneous tumours, like pancreatic ductal adenocarcinoma (PDAC) however, the characterisation of cancer stem cells has led to controversial results due to the lack of consensus on specific markers. (lu.se)
In addition, RT-qPCR and Western blot experiments were employed to investigate the tumor stem cell markers OCT4 and SOX2 expression levels in GCSCs. (bvsalud.org)
Zymography assay demonstrated that KLF6 inhibited the activities of matrix metalloproteinase 9 (MMP-9) and weakened the expression of mesenchymal markers, such as snail, slug, and vimentin. (medsci.org)
The objective of this article was to evaluate how these chemotherapeutic regimens affect the proportion of cancer stem cells and the expression of stemness markers. (isciii.es)

Here, we targeted an intracellular inhibiting protein 'cytokine inducible SH2-containing protein' (CISH) in NK cells to evaluate the impact on their functions and antitumor properties. (bmj.com)
In human NK cells, CISH deletion also favors NCR signaling and antitumor functions. (bmj.com)

We previously reported a MYC-centered regulatory network in mouse embryonic stem (ES) cells that accounts for the similarity between the ES cells and cancer cells, and is associated with a worse prognosis in cancer, including cancers of the breast [ 20 ]. (nature.com)
For example, RAD18 plays a vital role in regulating the DNA stability of embryonic stem cells and cellular homeostasis in highly prolific cells [ 6 ]. (nature.com)
Profoundly different patterns of potency and division are exhibited by mammalian embryonic and adult stem cells. (iospress.com)
Mammalian embryonic stem cells would seem adapted to rapid proliferation, functioning in part to enclose yolk or to acquire access to maternal resources. (iospress.com)
The cellular products of embryonic stem cells routinely come under global influences and give rise to the cells of germ layers and organ rudiments. (iospress.com)

Cancer stem cells-origins and biomarkers: perspectives for targeted personalized therapies[J]. Front Immunol, 2020, 11: 1280. (magtechjournal.com)

Methods To further understand CISH functions in NK cells, we developed a conditional Cish-deficient mouse model in NK cells ( Cish fl/fl Ncr1 Ki/+ ). (bmj.com)
3D cell culture models may be generally classified into two principal categories based on method: 1) scaffold-based methods using hydrogels or structural scaffolds and 2) scaffold-free approaches using freely floating cell aggregates, typically referred to as spheroids. (sigmaaldrich.com)

Human adipose tissue-derived stem cells (hASCs) are attractive cells for therapeutic applications and are currently being evaluated in multiple clinical trials. (biomedcentral.com)
The regenerative effectiveness of cell-assisted lipotransfer is dependent on the presence of adipose tissue-derived stem cells (ADSCs). (archive.org)

Clonal formation assay analyzed the change of cell proliferation potency. (medscimonit.com)
Overexpression of ST6GAL1 resulted in not only, increased proliferation and colony formation but iv also, decreased cell death when treated with IFN-γ and TNF-α. (uab.edu)
As an important stress-response mechanism, autophagy plays crucial role in the tumor formation and drug resistance of cancer cells including osteosarcoma (OS). (biomedcentral.com)
Common examples include cancer tumorspheres, which enable the study of oxygen gradients and nutrient access in tumor formation. (sigmaaldrich.com)
Trisomy 8+ cells showed a significant positive correlation with apoptotic CD34+ cells and capacity for colony formation. (who.int)

Promotes neurogenesis in human induced pluripotent stem cells (iPSCs) when used in combination with SB 431542 (Cat. (tocris.com)

The cell viability was examined with CCK8 assay. (biomedcentral.com)

CISH deletion favors NK cell accumulation to the primary tumor, optimizes NK cell killing properties and decreases TIGIT immune checkpoint receptor expression, limiting NK cell exhaustion. (bmj.com)

We after that examined tumor cell death when pressured KLF9 manifestation was combined with a variety of anti-tumor reagents, including chemotherapeutic medicines and epigenetic modulators. (immune-source.com)

Stably introduced into cancer cell lines, the system reports on elevated endogenous ADAR1 editing activity induced by interferon as well as knockdown of ADAR1 and ADAR2. (cancerindex.org)
Knockdown of BRCA1 and p63 leads to enhanced proliferation of breast cancer cell lines and increased stem cell numbers as assayed for by mammosphere culture and Aldefluor assay. (biomedcentral.com)

Using patient-derived glioblastoma cells researchers found that glioma stem-like cells over-expressed O-acetyl-GD2. (stemcellsciencenews.com)

and (2) through the paracrine release of trophic factors to induce tissue repair by endogenous cells [ 6 ]. (biomedcentral.com)
Exposure to diethyl nitrosamine can induce more hepatic tumors in KLF6-/+ mouse than in wild-type animals [ 9 ]. (medsci.org)

In both 2D and 3D cultures, stemness marker gene expression is higher in CD117+ cells. (biorxiv.org)
Ki-67 expression and the proliferation kinetics were investigated. (biomedcentral.com)
This complete characterization of hASCs cultivated in pooled allogeneic human serum, a suitable xeno-free approach, shows that pooled allogeneic human serum provides a high proliferation rate, which can be attributed for the first time to C-MYC protein expression, and showed cell stability for safe clinical applications in compliance with good manufacturing practice. (biomedcentral.com)
Expression of BRCA1 or p63 in a background of low BRCA1 and p63 results in decreased cell proliferation. (biomedcentral.com)
In a p63 and BRCA1 null background, expression of S100A2 results in a reduction of cell proliferation. (biomedcentral.com)
KRAS and TrkB gene expression levels were evaluated using quantitative real time PCR with TaqMan (R) Assay in both subpopulations. (fapesp.br)
The autophagy level was evaluated by the mRNA expression of autophagy genes, the protein level of LC3II and p62, and the mean number of GFP-LC3 dot per cell. (biomedcentral.com)
Numerous studies demonstrated an association between higher Reg4 expression and tumor aggressiveness, intrinsic resistance to apoptotic death, and poor outcomes from GI malignancies. (wustl.edu)
Conversely, tumors with strong expression of the CSC marker ALDH1 had elevated expression of CD44S. (lu.se)
A high expression of the CD44v2-v10 isoform, which retain all variant exons, was correlated to positive steroid receptor status, low proliferation and luminal A subtype. (lu.se)
High expression of CD44S was associated with strong HER2 staining and also a subgroup of basal-like tumors. (lu.se)
Silencing PDL-1 expression suppressed CAFs' ability to promote stem cell-like properties and invasiveness of lung cancer cells, favoring chemoresistance. (cdc.gov)
Our results suggest that although trisomy 8 cells are in a pro-apoptotic state, they are checked by the enhanced expression of anti-apoptotic signals which provide them with their proliferative advantage. (who.int)

In this study, our aim was to evaluate the proliferation pattern as well as the resistance to spontaneous transformation of hASCs during expansion in a xeno-free culture condition. (biomedcentral.com)

Scientists describe consistent up-regulation of ccarbonic anhydrase 2 (CA2) not only in temozolomide (TMZ) resistant glioblastoma (GBM) stem-like cells on mRNA and protein level, but also in patient-matched clinical samples of first manifest and recurrent tumors. (stemcellsciencenews.com)
Although the exact function of BRCA1 remains to be defined, roles in DNA damage repair, cell cycle checkpoint control, transcriptional regulation and, more recently, ubiquitination have been inferred. (biomedcentral.com)

Although we previously reported a Reg4-mediated induction of EGFR activity in colorectal cancer cells, a direct interaction between Reg4 and EGFR was not observed. (wustl.edu)

It's not necessary to know anything about your system with regard to the receptor, nor what kind of assay you're going to run to monitor that activity," he pointed out. (genengnews.com)
Say you have a G-protein coupled receptor (GPCR) of unknown function-with this assay you can determine whether it effects a stimulatory or inhibitory response, or induces necrosis under your experimental conditions, Dr. Jansson proposed as an example. (genengnews.com)
The LG.3A10 antibody reacts with CD27, a 45 - 55 kDa member of the tumor necrosis factor receptor superfamily. (stemcell.com)

Anatomy15

Organisms3

Diseases5

Chemicals and Drugs13

Analytical, Diagnostic and Therapeutic Techniques and Equipment9

Phenomena and Processes9

Information Science1

Vitro18

Differentiation18

Vivo17

Metastasis12

Recurrence7

Progression12

Suppressor5

Apoptosis9

Microenvironment6

CSCs9

Xenograft4

Flow cytometry4

Tumorigenesis5

Transplantation7

Migration11

Self-renewal2

Pathways8

Colony formation assays1

Angiogenesis3

Phenotype8

Nude2

CD4420

Transwell1

Monoclonal2

Inhibition5

Signaling pathway1

Breast tumors1

Invasion1

Clonogenic2

Types of tumors1

Subpopulation3

Initiation1

Abstract1

Solid tumors2

Functional assays1

Sphere formation assay1

Significantly4

Proteins2

Growth12

Viability assay1

Tissues7

Metastatic2

Mice7

Inhibitors7

Metabolism2

Markers4

Antitumor2

Embryonic5

Biomarkers1

METHODS2

Adipose tissue-derived stem cells2

Formation5

Promotes1

CCK81

Primary tumor1

Anti-tumor1

Knockdown2

Glioma stem-like cells1

Induce2

Expression13

Resistance1

Regulation2

Induction1

Receptor3