Prokaryotes proteins genes bacterial. Medical search. Frequent questions

RNA codons and protein synthesis. (crossref.org)
Here we show that staphylococcal phages produce a structured RNA transcribed from the terminase subunit genes, termed CBASS-activating bacteriophage RNA (cabRNA), which binds to a positively charged surface of the CdnE03 cyclase and promotes the synthesis of the cyclic dinucleotide cGAMP to activate the CBASS immune response. (bvsalud.org)
It can function as an enzyme, scaffold various subcellular structures, and regulate gene expression through a variety of mechanisms, as well as act as a key component of the protein synthesis and splicing machinery. (cshlpress.com)
The gene series of rRNA can be conserved in bacterias throughout advancement extremely, due to their central part in proteins synthesis (16). (cancer-pictures.org)
Such interactions generally result in a decrease in protein synthesis, either by stimulating mRNA degradation or by inhibiting translation. (biomedcentral.com)
Many phototrophs are auxotrophic for the synthesis of vitamins that are provided by their bacterial partners, which in turn benefit from the released exudates. (dsmz.de)
Blasticidin S is a microbial antibiotic that inhibits protein synthesis in both prokaryotes and eukaryotes. (atheistsforhumanrights.org)
The transcribed region of a gene contains the template for synthesis of an RNA, which begins at the start point. (basicmedicalkey.com)
Because bacteria do not contain nuclei, ribosomes bind to mRNA as it is being transcribed, and protein synthesis occurs simultaneously with transcription. (basicmedicalkey.com)
Many bioY genes are located at loci encoding biotin biosynthesis or are unlinked to either biotin synthesis or other biotin transport genes. (tcdb.org)

The mobili- tamases that are located on plasmids and that have been zation of a chromosomal resistance gene to a plasmid is of particular clinical interest because they are rapidly an important event because the mobilized gene is now ca- spreading through clinical populations of bacteria. (cdc.gov)
Bacteriophage transduction is the major mechanism of horizontal gene transfer (HGT) among many bacteria. (bvsalud.org)
Despite phage replication in these matrices, lysis of the bacteria was slower than phage propagation, and no reduction of the bacterial population was observed. (bvsalud.org)
Phyletic distributions of eukaryotic signalling domains were studied using recently developed sensitive methods for protein sequence analysis, with an emphasis on the detection and accurate enumeration of homologues in bacteria and archaea. (embl.de)
We also widen the research to the genomes of all the bacteria, finding 227 entries in 12 bacterial phyla. (bvsalud.org)
ppGpp is an intracellular sensor that, in response to different types of stress, coordinates the rearrangement of the gene expression pattern of bacteria to promote adaptation and survival to new environmental conditions. (ibecbarcelona.eu)
The prokaryotic MORC proteins were widely, but sporadically, distributed in several distantly related bacteria such as proteobacteria, cyanobacteria and actinobacteria, as well as certain crenarchaea and euryarchaea [ 7 ]. (beds.ac.uk)
So far, such genes have not been found in any of the free living green sulfur bacteria, indicating a specific role in the interaction. (dsmz.de)
CRISPR-Cas was first discovered in prokaryotes (bacteria and other single-cell organisms that lack nuclei) and scientists including those in Zhang's lab have long wondered whether similar systems exist in eukaryotes. (mit.edu)
Unlike CRISPR proteins, Fanzor enzymes are encoded in the eukaryotic genome within transposable elements, and the team's phylogenetic analysis suggests that the Fanzor genes have migrated from bacteria to eukaryotes through so-called horizontal gene transfer. (mit.edu)
The main problem with this idea is that, not only does no such creature exist, but eukaryotes also contain molecular similarities to both bacteria and archaea-prokaryotes that are found in completely separate domains of cellular life. (icr.org)
In contrast to the free-living, or planktonic, bacteria commonly studied in the lab, most prokaryotes in nature settle down in complex communities, often consisting of several interacting species. (the-scientist.com)
Biofilms form when groups of bacteria cover themselves in a sticky mixture of sugars, protein, and DNA. (the-scientist.com)
Bacteria sense these microenvironments and adapt by up or down regulating the expression of certain genes to influence different cell processes. (the-scientist.com)
Some bacteria have the bioY gene clustering with bioB , which encodes biotin synthase, an enzyme that converts dethiobiotin to biotin. (tcdb.org)
2009 have a dedicated energizing module, but in 459 cases distributed among almost 100 gram-positive bacteria, different and unrelated substrate capture proteins share the same energy-coupling module. (tcdb.org)

VapBC operons have been found in distantly related prokaryotes, including the pathogens Leptospira interrogans, Mycobacterium tuberculosis and Piscirickettsia salmonis. (wikipedia.org)
These organisms are unique in that they are only distantly related to other bacterial sulfate reducers, and because they can grow at extremely high temperatures. (up.ac.za)
Both of the integral membrane protein constituents of these systems may be distantly related, and in this respect they resemble typical ABC porters. (tcdb.org)
HGT allows even distantly related species to share genes, influencing their phenotypes. (pressbooks.pub)

The functioning of this system seems to involve integration of fragments of foreign genes into archaeal and bacterial chromosomes yielding heritable immunity to the respective agents. (biomedcentral.com)
Concept of gene, Prokaryotes as genetic system, Prokaryotic and eukaryotic chromosomes, methods of gene isolation and identification, Split genes, overlapping genes and pseudo genes, Organization of prokaryotic and eukaryotic genes and genomes including operan, exon, intron, enhancer promoter sequences and other regulatory elements. (nipaers.com)
In eukaryotes , the DNA molecules that make up the genome are packaged with proteins into chromosomes, each of which contains a single linear DNA molecule. (jrank.org)

It is proposed that unique inserts of CRISPR, some of which are homologous to fragments of bacteriophage and plasmid genes, function as prokaryotic siRNAs (psiRNA), by base-pairing with the target mRNAs and promoting their degradation or translation shutdown. (biomedcentral.com)
We identify the prokaryotic MORCs and establish that the MORC family belongs to a larger radiation of several families of GHKL proteins (paraMORCs) in prokaryotes. (beds.ac.uk)
The prokaryotic and eukaryotic MORC homologs were unified to the exclusion of the other GHKL proteins by a specific GhXhhpXXRhl motif (h: hydrophobic, p: polar, X: any) in the S5-fold domain (Fig. 1 ). (beds.ac.uk)
That work also highlighted similarities between prokaryotic OMEGA systems and Fanzor proteins in eukaryotes, suggesting that the Fanzor enzymes might also use an RNA-guided mechanism to target and cut DNA. (mit.edu)
It is thought that HGT is more prevalent in prokaryotes but that only a small fraction of the prokaryotic genome may be transferred by this type of transfer at any one time. (pressbooks.pub)

Comparative-genomic analysis of CRISPR and cas genes leads to the hypothesis that the CRISPR-Cas system (CASS) is a mechanism of defense against invading phages and plasmids that functions analogously to the eukaryotic RNA interference (RNAi) systems. (biomedcentral.com)
Serially diluted bacterial genomic DNA was utilized to generate the typical curve. (cancer-pictures.org)
Symbiosis genes" of the epibiont were identified by genomic approaches. (dsmz.de)
Instead, they noted that 'we provide an integrative and robust classification, useful for future genomic and functional studies on this crucial eukaryotic gene family. (icr.org)

Sequence comparison between wild-type hfsH from Caulobacter crescentus [37] and our optimized gene for Escherichia coli . (igem.org)
Sequence comparison between wild-type hfsG from Caulobacter crescentus [37] and our optimized gene for Escherichia coli . (igem.org)
The gene encoding ArsR/methyltransferase fusion protein, arsR M , was amplified and expressed in Escherichia coli BL21 (DE3), and this strain showed resistance to arsenic in the present of 0.25-6 mM As(III), aresenate, or pentavalent roxarsone. (biomedcentral.com)

G ene "expression" translates molecule strings (replicable nucleic acid codes) into the proteins and enzymes needed to run a cell. (umsl.edu)
En revanche, elle dispose de nombreux enzymes intervenant dans le métabolisme des nucléotides et un ribosome primordial permettant la synthèse de protéines[27] mais de façon encore peu fiable. (statesindex.org)
Co-first author Makoto Saito of the Zhang lab led the biochemical characterization of the Fanzor proteins, showing that they are DNA-cutting endonuclease enzymes that use nearby non-coding RNAs known as ωRNAs to target particular sites in the genome. (mit.edu)
Secondly, those proteins which are present in soluble form in the cytoplasm often are subject to degradation by host specific enzymes, thus reducing the amounts of active protein that can be recovered. (justia.com)
Enzymes known to the prior art as "proteolytic enzymes" have been found to be particularly well suited for the cleavage of fusion proteins. (justia.com)
Genes are transcribed by enzymes called RNA polymerases that generate a single-stranded RNA identical in sequence (with the exception of U in place of T) to one of the strands of the double-stranded DNA. (basicmedicalkey.com)
A very natural place to study gene detecting and metabolizing lactose, it is known that the overall regulation is in the metabolism of the cell, and then specifically in effect of expressing the lac genes in vain is a drop in the growth rate the regulation of genes that code for enzymes and transporter of as much as 5% [1,6]. (lu.se)

Two years ago, Zhang lab members discovered a class of RNA-programmable systems in prokaryotes called OMEGAs, which are often linked with transposable elements, or "jumping genes," in bacterial genomes and likely gave rise to CRISPR-Cas systems. (mit.edu)

SHV have been chromosomally located since the pre-antimicro- the expression of antibiotic-resistance genes, DNA uptake bial drug era ( 15 ), and they may have been mobilized to through transformation, or the process of conjugation. (cdc.gov)
New Facets in Mycobacterium and Malaria parasite biology, Regulation of gene expression. (infinitecourses.com)
Consider this in light of the above-mentioned conclusion from molecular biology, that informed gene-expression in eukaryotes is crucial to the development and sustainable operation of individual metazoans. (umsl.edu)
Here we describe our studies on the genetic organization of phages infecting Staphylococcus aureus, a pathogen of human importance, by using an assembly of tools for gene annotation, identification of expression components, and phylogeny analysis. (bvsalud.org)
TMs decreased the association of HuR with genes involved in chemotaxis and immune response, including Cxcl10, Il1b and Cd40, reducing their expression and protein secretion in primary murine bone marrow-derived macrophages and in an LPS-induced peritonitis model. (bvsalud.org)
We checked the expression at the transcript and protein level, cloned the gene and expressed the recombinant protein. (bvsalud.org)
Meanwhile, FZ supported systemic expression of fluorescent proteins mNeonGreen and mCherry in N. benthamiana and soybean. (researchsquare.com)
First described to modulate metabolic adaptive responses, ppGpp modulates the expression of genes belonging to very diverse functional categories. (ibecbarcelona.eu)
Our data further support the role of ppGpp participating in the coordinated regulation of the expression of bacterial factors required during infection. (ibecbarcelona.eu)
Within the research in Molecular Biology, one important field along the years has been the analyses on how prokaryotes regulate the expression of their genes and what the consequences of these activities are. (ibecbarcelona.eu)
These alterations include multinucleation and the expression by infected cells of mRNA for factors required for osteoclastogenesis: the chemokines monocyte chemotactic protein 1 (MCP-1), macrophage inflammatory protein 1 gamma (MIP-1? (edu.au)
Expression of genes for the osteoclast markers calcitonin receptor (CTR), cathepsin K (CTSK) and tartrate-resistant acid phosphatase (TRAP) was also increased by B. pseudomallei-infected, but not by B. thailandensis-infected cells. (edu.au)
The expression by B. pseudomallei-infected cells of these chemokine and osteoclast marker genes was remarkably similar to cells treated with RANKL, a stimulator of osteoclastogenesis. (edu.au)
Mutation of lfpA significantly reduced expression of the tested host genes, relative to the response to wild-type B. pseudomallei. (edu.au)
Quorum sensing is a community behaviour that enables microorganisms to communicate, perceive and monitor population density, and modulate gene expression by producing and responding to diffusible signal molecules. (igem.org)
Reaching the critical level of quorum sensing molecules enables therefore a coordinated expression of specific genes, for example the luminescence genes in V. fischeri or the competence in S. pneumoniae. (igem.org)
Loss of ybeY function, which results in a marked resistance to HU, had global affects on sRNA-mediated gene expression. (biomedcentral.com)
Small regulatory RNAs (sRNAs) play key roles in modulating gene expression in both prokaryotes and eukaryotes. (biomedcentral.com)
Generation of a stable cell line refers to the process of developing homogenous populations of cells that demonstrate expression of a transfected gene insert. (atheistsforhumanrights.org)
Construction and Analysis of Gene Co-Expression Network in the Pathogenic Fungus Ustilago maydis. (openwetware.org)
Photoregulation and phytochrome regulation of nuclear and chloroplastic gene expression. (nipaers.com)
Production of the fusion polypeptide involves the introduction of the hybrid gene into a biological host cell system, for example yeast cells, which permits the expression and accumulation of the fusion polypeptide. (justia.com)
Expression of truncated bio transport operons, each with one gene deleted, resulted in stable BioMN complexes but revealed only low amounts of BioMY and BioNY aggregates in the absence of the respective third partner. (tcdb.org)

Comparisons of the domain distributions in eukaryotes and prokaryotes enabled distinctions to be made between the domains originating prior to the last common ancestor of all known life forms and those apparently originating as consequences of horizontal gene transfer events. (embl.de)
Despite the hundreds of millions of years of evolutionary history separating eukaryotes and prokaryotes, the features of the replication process have been highly conserved between them. (jrank.org)

Other bacterial sRNAs, referred to as cis -encoded sRNAs, are present in close proximity to their targets, such as upstream, opposite of the 5′ UTR of the target, or between two genes in an operon [ 2 ]. (biomedcentral.com)
In the case of the lac operon of E. coli, a well-studied system for whether a gene is regulated. (lu.se)
Here, the function of regulation is quite clear: expressing energy and carbon, that a number around 0.2% would be the right genes at the right time will enable the cell to make the expected, and that the difference is more or less specific to the lac most of the resources within its reach, by maximizing the uptake operon [7]. (lu.se)

To provide an understanding of ribosome structure and mRNA translation in both prokaryotes and eukaryotes. (bath.ac.uk)
The most extensively characterized class of bacterial sRNAs are the trans -encoded sRNAs that are encoded distant from the genes for their mRNA targets and that typically have only limited complementarity (10-30 nt) [ 2 ]. (biomedcentral.com)
Exons, which contain the coding sequences for the proteins, are separated in pre-mRNA by introns , regions that have no coding function. (basicmedicalkey.com)

Comparative genomics of DNA-binding transcription factors in archaeal and bacterial organisms. (openwetware.org)

To introduce the process of transcription in prokaryotes. (bath.ac.uk)
Although the majority of VWA-containing proteins are extracellular, the most ancient ones present in all eukaryotes are all intracellular proteins involved in functions such as transcription, DNA repair, ribosomal and membrane transport and the proteasome. (embl.de)
Contributing to the interest of the present topic is the fact that modulation of gene activity involves the sensing of intra- and inter-cellular conditions, DNA binding and DNA dynamics, and interaction with the replication/transcription machinery of the cell. (ibecbarcelona.eu)
When the population increases, the signal concentration elevates and at a threshold level the acyl-HSLs come in interaction with transcription factors like the LuxR proteins. (igem.org)
2023. Editorial: Role of Transcription Factors and Sigma Factors in Bacterial Stress. (openwetware.org)
the cell may regulate the transcription of individual genes through regulatory molecules (ex. (powershow.com)
A gene is a segment of DNA that functions as a unit to generate an RNA product or, through the processes of transcription and translation, a polypeptide chain. (basicmedicalkey.com)

Cyclic oligonucleotide-based antiphage signalling systems (CBASS) protect prokaryotes from viral (phage) attack through the production of cyclic oligonucleotides, which activate effector proteins that trigger the death of the infected host1,2. (bvsalud.org)

RESEARCH analysis of the CTX-Ms that included a representative sam- site in a gene, among-site variation in evolutionary rate was pling of other class A -lactamases. (cdc.gov)
This genetic inventory was sufficient to place the cell within the ToL using multigene phylogenetics and provided preliminary insights into the complex evolutionary history of horizontal gene transfer (HGT) in the MAST-4 lineage. (nature.com)
Using these partial data we included the MAST-4 cell in the ToL using multigene phylogenetics and gained insights into its complex evolutionary history of horizontal gene transfer (HGT). (nature.com)
The unique inserts in CRISPR show virtually no similarity even between closely related bacterial strains which suggests their rapid turnover, on evolutionary scale. (biomedcentral.com)
However, it appears that this inheritance is extremely unstable on the evolutionary scale such that the repertoires of unique psiRNAs are completely replaced even in closely related prokaryotes, presumably, in response to rapidly changing repertoires of dominant phages and plasmids. (biomedcentral.com)
In Staphylococcus aureus, the phage-mediated acquisition of mobile genetic elements (MGEs) that encode virulence and antibiotic resistance genes largely contribute to its evolutionary adaptation and genetic plasticity. (bvsalud.org)
Notably we observed a partial evolutionary divergence between the RNP1 and RNP2 conserved regions present in the prokaryotes in comparison to the metazoan consensus sequence. (bvsalud.org)
2014). Means the begining of a new science the Evolutionary-Developmental Biology (EVO-DEVO) recently the most developping part of Biology, involves all modern innovations and methods to study genetical, epigenetical regulations and system based thinking about the varieties of gene and cell functions. (pte.hu)
So, how did the authors explain the incredible complexity found across the spectrum of life in myosin gene content that had no clear evolutionary patterns? (icr.org)
They explained it by 1) convergence (the sudden and simultaneous appearance of a gene with no evolutionary patterns in different taxa), 2) lineage-specific expansions (different myosin gene complements found in different creatures), and 3) gene losses (missing genes that evolutionists thought should have been there). (icr.org)
None of these ideas actually explain why there is no evolutionary pattern of simple-to-complex in myosin gene content across the spectrum of life. (icr.org)

Functional prediction analyses suggested that ArsR M is a difunctional protein with transcriptional regulation and methyltransferase activities. (biomedcentral.com)
Gene regulation, RNA processing and Post transcriptional modifications. (nipaers.com)
A transcriptional regulator in prokaryotes which, when activated by binding cyclic AMP, acts at several promoters. (bvsalud.org)
Transcriptional regulation of the genes in metabolic pathways is a highly successful strategy, which is virtually universal in microorganisms. (lu.se)
With a minimalist model of metabolism, cell growth and transcriptional regulation in a microorganism, we explore how the interaction between environmental conditions and gene regulation set the growth rate of cells in the phase of exponential growth. (lu.se)
INTRODUCTION broken, which in realistic situations can severly constrain the Transcriptional regulation of effector genes is a highly successful regulatory options. (lu.se)

VapBC (virulence associated proteins B and C) is the largest family of type II toxin-antitoxin system genetic loci in prokaryotes. (wikipedia.org)
Once thought to be just a messenger that allows genetic information encoded in DNA to direct the formation of proteins, RNA (ribonucleic acid) is now known to be a highly versatile molecule that has multiple roles in cells. (cshlpress.com)
The transfected gene integrates into the genome of the host cell, and as a result, are able to express the transfected genetic material. (atheistsforhumanrights.org)
A major aim of the Zhang lab is to develop genetic medicines using systems that can modulate human cells by targeting specific genes and processes. (mit.edu)
Gene cloning and sub-cloning strategies, chromosome walking, genetic transformation, Basis of animal cloning. (nipaers.com)
These techniques are based upon the preparation of hybrid genes, i. e. genes comprising genetic material encoding the polypeptide of interest linked to genetic material additional to the gene of interest. (justia.com)
Typically, when we consider genetic transfer, we think of vertical gene transfer, the transmission of genetic information from generation to generation. (pressbooks.pub)
Vertical gene transfer is by far the main mode of transmission of genetic information in all cells. (pressbooks.pub)
When prokaryotes and eukaryotes reproduce asexually, they transfer a nearly identical copy of their genetic material to their offspring through vertical gene transfer. (pressbooks.pub)
In prokaryotes, horizontal gene transfer (HGT), the introduction of genetic material from one organism to another organism within the same generation, is an important way to introduce genetic diversity. (pressbooks.pub)
Many scientists believe that HGT and mutation are significant sources of genetic variation, the raw material for the process of natural selection, in prokaryotes. (pressbooks.pub)

VagC was predicted to encode a 12kDa polypeptide, while vagD encoded a smaller 10kDa protein. (wikipedia.org)
Three of the six up-regulated genes encode potential zinc transporter proteins (ZnuA1, ZnuB1, ZnuC1) suggesting involvement of s479 in regulation of zinc transport. (uni-stuttgart.de)
Scientists believe that the study of genes that encode the proteins for molecular motors will help solve the mysteries of evolution. (icr.org)

In the hyperthermophilic archaean Sulfolobus solfataricus, for example, a vapBC gene cassette is thought to regulate heat shock response. (wikipedia.org)
A gene also includes regions of DNA that regulate production of the encoded product, such as a promoter region. (basicmedicalkey.com)

Members of the conserved Argonaute (Ago) protein family provide defence against invading nucleic acids in eukaryotes in the process of RNA interference. (nih.gov)

To explain the various pathways of intracellular and extracellular protein turnover. (bath.ac.uk)
VWA domains in extracellular eukaryotic proteins mediate adhesion via metal ion-dependent adhesion sites (MIDAS). (embl.de)
[2] [3] The cells within the biofilm produce the EPS components, which are typically a polymeric conglomeration of extracellular polysaccharides , proteins , lipids and DNA . (wikipedia.org)
Some individuals focus on reproduction to expand the colony, while others specialize in construction, oozing polysaccharides and proteins that make up the extracellular matrix. (the-scientist.com)

Protein biosynthesis in prokaryotes and ekaryotes. (nipaers.com)

The second one is a methylesterase that controls the adaptation of the methyl-accepting chemotaxis proteins or chemoreceptors (MCPs). (igem.org)

In molecular biology, generalized transduction is routinely used as a technique to manipulate and construct bacterial strains. (bvsalud.org)
Interestingly, s479 is not only encoded next to CRISPR-cas genes but the mature s479 contains a crRNA-like 5´ handle and experiments with Cas protein deletion strains indicate maturation by Cas6 and interaction with Cas proteins. (uni-stuttgart.de)
This is a false colored mesoscopic image of two strains of E. coli expressing different fluorescent proteins. (the-scientist.com)

The blast clade genomes contain more secretome and avirulence effector genes, which likely play key roles in the interaction between Pyricularia species and their plant hosts. (nature.com)

The new CPSMV-derived FZ vector provides a convenient and versatile soybean functional genomics tool that is expected to accelerate the characterization of soybean genes controlling crucial productivity traits. (researchsquare.com)
Virus-induced gene silencing (VIGS) is an alternative functional genomics tool for soybean because it does not involve the generation of inheritable changes. (researchsquare.com)
Phandango: an interactive viewer for bacterial population genomics. (cdc.gov)

Ancient ancestry and horizontal gene transfer. (embl.de)
Little evidence of inter-phylum horizontal gene transfer (HGT) was detected in Magnaporthales. (nature.com)
There are three prokaryote-specific mechanisms leading to horizontal gene transfer in prokaryotes. (pressbooks.pub)

The association of the cas genes with CRISPR and, especially, the presence, in CRISPR units, of unique inserts homologous to phage and plasmid genes make us abandon this hypothesis. (biomedcentral.com)
Here, we browsed the A. baumannii proteome for homologous proteins to the human HuR(ELAVL1), an RNA binding protein containing three RRMs. (bvsalud.org)
When evidence suggests that homologous membrane transport proteins of the ABC2 type couple transport to ATP hydrolysis using a homologue of the ABC-type ATPases, we list these proteins in the ABC superfamily. (tcdb.org)

The highly conserved bacterial YbeY RNase has structural similarities to the MID domain of AGOs. (biomedcentral.com)

There are two major, distinct forms of regulatory small RNAs involved in eukaryotic gene silencing: small interfering (si) RNAs and micro (mi) RNAs. (biomedcentral.com)
The book goes on to discuss the evolution of ribosomes and the functions of RNPs, before reviewing the recent work that has revolutionized our understanding of gene regulation by non-coding RNAs, including miRNAs and siRNAs. (cshlpress.com)

Figure 7.1 (a) Nasal sinus cells (viewed with a light microscope), (b) onion cells (viewed with a light microscope), and (c) Vibrio tasmaniensis bacterial cells (seen through a scanning electron microscope) are from very different organisms, yet all share certain characteristics of basic cell structure. (infolearners.com)
There is a strong emphasis on the structure of molecules, particularly proteins, which are the nanoscale machines that carry out most processes in living organisms. (bbk.ac.uk)
Mycoplasma species are the smallest free-living organisms and are unique among prokaryotes in that they lack a cell wall. (medscape.com)

Model of acyl-HSL quorum sensing in a single generalized bacterial cell. (igem.org)

RNA and ribonucleoprotein immunoprecipitation sequencing revealed that LPS induces a strong coupling between differentially expressed genes and HuR-bound genes, and TMs reduced such interactions. (bvsalud.org)
Müller, J. , Overmann, J. (2011) Close interspecies interactions between prokaryotes from sulfureous environments. (dsmz.de)

Corollaries of this finding are that, even among closely related prokaryotes, the most commonly encountered phages and plasmids are different and/or that the dominant phages and plasmids turn over rapidly. (biomedcentral.com)
Prokaryotes commonly use a histidine-aspartate phosphorelay (HAP) system consisting of a histidine protein kinase (HPK) and a response regulator (RR). (igem.org)
Consistent with a chromatin-associated role, the MORCs display fusions to several DNA- and peptide-binding domains, which are commonly found other eukaryotic chromatin proteins [ 5 ]. (beds.ac.uk)

All archaeal and many bacterial genomes contain Clustered Regularly Interspaced Short Palindrome Repeats (CRISPR) and variable arrays of the CRISPR-associated ( cas ) genes that have been previously implicated in a novel form of DNA repair on the basis of comparative analysis of their protein product sequences. (biomedcentral.com)
In contrast, many genes underwent positive selection in this order and the majority of these sequences are clade-specific. (nature.com)
Researchers had hoped to find that matters would be clarified by myosin proteins derived from the DNA sequences of different single-celled eukaryotes, such as flagellated protozoa (protozoa with a whip-like tail), amoeboid protozoa, and algae. (icr.org)
In a structural gene, the transcribed region contains the coding sequences that dictate the amino acid sequence of a polypeptide chain. (basicmedicalkey.com)

Computational approaches to the prediction of protein folding (practical) (16 hr). (bath.ac.uk)

The provenance and biochemical roles of eukaryotic MORC proteins have remained poorly understood since the discovery of their prototype MORC1, which is required for meiotic nuclear division in animals. (beds.ac.uk)

VapC is strongly inhibited by direct protein interaction with VapB, its cognate antitoxin. (wikipedia.org)
2016). Dynamic metabolic exchange governs a marine algal-bacterial interaction. (dsmz.de)

The blasticidin S-resistance gene (bsr), isolated from Bacillus cereus K55-S1 strain, was inserted into pSV2 plasmid vector and introduced into cultured mammalian cells by transfection. (atheistsforhumanrights.org)

VapBC operons consist of two genes: VapC encodes a toxic PilT N-terminus (PIN) domain, and VapB encodes a matching antitoxin. (wikipedia.org)

This island contains ribosomal proteins or RNA related elements and may indicate a False Positive Prediction! (up.ac.za)

The X-ray and NMR structural characterization of the recombinant AB-Elavl revealed that the protein maintained the typical ß1α1ß2ß3α2ß4 and three-dimensional organization of eukaryotic RRMs. (bvsalud.org)
The common ancestor of these GHKL proteins, MutL and topoisomerase ATPase modules appears to have catalyzed structural reorganization of protein complexes and concomitant DNA-superstructure manipulations along with fused or standalone nuclease domains. (beds.ac.uk)

Additionally, in a phylogenetic tree versions from the same bacterial lineage (e.g. proteobacteria) often do not group together. (beds.ac.uk)
Phylogenetic inference of Coxiella burnetii by 16S rRNA gene sequencing. (cdc.gov)

The genomes of bacterial cells ( prokaryotes ), which lack a nucleus, are typically circular DNA molecules that associate with special structures in the cell membrane. (jrank.org)

Using de novo gene prediction, we identified 6,996 protein-encoding genes in the MAST-4 genome. (nature.com)
Analysis of the genome data using ab initio gene prediction identified 6,996 protein-encoding genes in the genome of the isolate. (nature.com)

The fictional LECA creature represents the final stage of a transition between a bacterial-archaeal prokaryote (the smallest and simplest organism) and a one-celled eukaryote (a cell with a nucleus and other organelles). (icr.org)
Before a cell divides, DNA must be precisely copied, or "replicated," so that each of the two daughter cells can inherit a complete genome, the full set of genes present in the organism. (jrank.org)

Upregulation of these genes in the deletion strain was confirmed by northern blot and proteome analyses. (uni-stuttgart.de)
2009 identified 21 families of these substrate capture proteins, each with a different specificity predicted by genome context analyses. (tcdb.org)

Other applications that rely on MDA of single cells include targeted metagenomics, whereby marker genes are PCR-amplified from the DNA sample to decipher their distribution in ecosystems or larger fragments of DNA are assembled for analysis of gene content 11 , 17 . (nature.com)
Bacterial cells (prokaryotes, i.e. cells without a nucleus) have fewer ways to control which codes they express than do the eukaryotic cells of plants and animals. (umsl.edu)
in eukaryotic cells the most important of these proteins are the histones . (wikipedia.org)
Prokaryotes have attracted the interests of researchers not only because the processes taking place in their world are important to cells, but also because many of the effects often can be readily measured, both at the single cell level and in large populations. (ibecbarcelona.eu)
Bacterial translocation Bacterial translocation was established as bacterial fill in liver organ cells and was quantified by real-time PCR utilizing the 16S rRNA gene consensus series. (cancer-pictures.org)
PCR-derived bacterial matters had been indicated as nanogram bacterial DNA per gram mouse liver organ cells. (cancer-pictures.org)
Transcriptome studies of wild type Haloferax cells and the deletion mutant revealed up-regulation of six genes in the deletion strain, showing that the sRNA has a clearly defined function. (uni-stuttgart.de)
They showed that Fanzor proteins use RNA as a guide to target DNA precisely, and that Fanzors can be reprogrammed to edit the genome of human cells. (mit.edu)
Molecular motors are important features of eukaryotic cells that are formed by a variety of protein types. (icr.org)
When purified from its host cells or from heterologous E. coli , SeAgo is loaded with small guide DNAs whose formation depends on the endonuclease activity of the argonaute protein. (nih.gov)

Intracellular VWA domains and homologues in prokaryotes have recently been identified. (embl.de)
Previously undetected bacterial homologues were identified for# plant pathogenesis-related proteins, Pad1, von Willebrand factor type A, src homology 3 and YWTD repeat-containing domains. (embl.de)
These membrane proteins are homologues to ABC-2 exporters. (tcdb.org)

The two components of this plasmidic system were originally named vagC and vagD (virulence-associated gene) for the toxin and antitoxin genes respectively. (wikipedia.org)
Gene regulatory network inference and gene module regulating virulence in Fusarium oxysporum. (openwetware.org)

The method involves expressing the recombinant polypeptide as a fusion protein with a pro-peptide. (justia.com)
The pro-peptide-polypeptide fusion protein can be cleaved and the recombinant polypeptide released under the appropriate conditions. (justia.com)
The preparation of valuable recombinant (genetically engineered) polypeptides, for example pharmaceutical proteins, relies frequently on techniques which involve the production of these polypeptides as fusion or hybrid proteins. (justia.com)

b) In transduction, a bacteriophage injects DNA that is a hybrid of viral DNA and DNA from a previously infected bacterial cell. (pressbooks.pub)

Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. (cdc.gov)

Furthermore, we identified and characterized lfpA (for lactonase family protein A) in B. pseudomallei, which shares significant sequence similarity with the eukaryotic protein 'regucalcin', also known as 'senescence marker protein-30' (SMP-30). (edu.au)
To unravel the provenance and relationships of the MORC family, we initiated sequence profile searches using the PSI-BLAST program seeded with eukaryotic MORC proteins. (beds.ac.uk)

2023. The gene regulatory network of Staphylococcus aureus ST239-SCCmecIII strain Bmb9393 and assessment of genes associated with the biofilm in diverse backgrounds. (openwetware.org)
Homology-based reconstruction of regulatory networks for bacterial and archaeal genomes. (openwetware.org)
Cell surface cyclic AMP receptors are not included (CYCLIC AMP RECEPTORS), nor are the eukaryotic cytoplasmic cyclic AMP receptor proteins, which are the regulatory subunits of CYCLIC AMP-DEPENDENT PROTEIN KINASES. (bvsalud.org)

2023 ). (1) the BioMNY complex with ATPase (BioM) and transmembrane protein (BioN). (tcdb.org)

Biofilms are thought to have arisen during primitive Earth as a defense mechanism for prokaryotes, as the conditions at that time were too harsh for their survival. (wikipedia.org)

Cyclic AMP receptor protein was originally identified as a catabolite gene activator protein. (bvsalud.org)

[10] [11] A cell that switches to the biofilm mode of growth undergoes a phenotypic shift in behavior in which large suites of genes are differentially regulated . (wikipedia.org)

Adventure Story Chests, While we don't know what these organsims were like genetically, we do know that they had no true nucleus and were morphologically similar to … He presents an original scenario which envisages the transition of prokaryotes to eukaryotes in a quasi-solid milieu in which loss of the bacterial wall is followed by the expansion of the cytoplasmic membrane to permit phagocytosis. (statesindex.org)
Eukaryotic genes are transcribed in the nucleus by three different RNA polymerases , each principally responsible for one of the major classes of RNA. (basicmedicalkey.com)

This similarity between genes and ideas is not just metaphorical. (umsl.edu)

prokaryote October 22, 2016 by 3.8 Billion Years. (statesindex.org)

In eukaryotes, Argonaute (AGO) proteins discharge such functions. (biomedcentral.com)

Anatomy12

Organisms42

Diseases3

Chemicals and Drugs69

Analytical, Diagnostic and Therapeutic Techniques and Equipment22

Psychiatry and Psychology1

Phenomena and Processes103

Disciplines and Occupations3

Technology, Industry, Agriculture1

Information Science10

Health Care5

Geographicals2

Synthesis10

Bacteria16

Distantly related4

Chromosomes3

Prokaryotic5

Genomic4

Escherichia3

Enzymes7

Transposable Elements1

Expression23

Eukaryotes and prokaryotes2

Operon3

MRNA3

Archaeal and bacterial1

Transcription7

Protect prokaryotes1

Evolutionary11

Transcriptional6

Genetic11

Encode3

Regulate2

Nucleic acids1

Extracellular4

Biosynthesis1

Chemotaxis1

Strains3

Effector genes1

Genomics3

Horizontal3

Homologous3

Highly conserved1

RNAs2

Organisms3

Generalized bacterial cell1

Interactions2

Commonly3

Sequences4

Computational1

Roles1

Interaction2

Plasmid1

Operons1

Ribosomal1

Structural2

Phylogenetic2

Molecules1

Prediction2

Organism2

Analyses2

Cells10

Homologues3

Virulence2

Recombinant3

Transduction1

Taxonomy1

Sequence2

Regulatory3

20231

Defense1

Cyclic1

Differentially1

Nucleus2

Similarity1

20161

Argonaute1