• The phylogenetic analysis of the strain was based on 16S rRNA gene sequencing and showed that Paenibacillus sp. (asm.org)
  • The original designs of most of the PCR primers used for the analysis of 16S rRNA genes were based on known sequences deposited in public databases. (frontiersin.org)
  • classification of new phytoplasmas associated with diseases of strawberry in florida, based on analysis of 16s rrna and ribosomal protein gene operon sequences. (liverpool.ac.uk)
  • analysis of 16s rrna gene sequences amplified by pcr indicated that the symptoms were associated with infection by a phytoplasma, designated strawberry yellows (strawy) phytoplasma. (liverpool.ac.uk)
  • For example, a complete 16S rRNA gene sequence can be obtained by analyzing the sequences of genomes or large genome fragments, providing taxonomic information along with information on other functional genes. (frontiersin.org)
  • With shotgun metagenomic datasets covering an ever widening range of biomes, multiple studies have been conducted with the aim of recovering the genomes of prokaryotes, viruses and more recently eukaryotes 7 . (researchsquare.com)
  • Discovery of new bacterial species shows little sign of abating, demonstrating the continued need to improve the experimental methods and informatics tooling used to produce metagenome assembled genomes (MAGs) thereby allowing the full microbial diversity to be described. (researchsquare.com)
  • 13) The combined genetic contributions of these microbes - in excess of 1,000,000 protein-coding genes - provide traits not encoded in our own genomes. (mpkb.org)
  • The genomes' seasonal dynamics follow phylogenetic patterns, but with fine-grained lineage-specific variations, reflected in gene-content. (biomedcentral.com)
  • We describe the gene content, temporal dynamics and biogeography of a large set of new bacterioplankton genomes assembled from metagenomes. (biomedcentral.com)
  • To understand these processes through comprehensive analyses of microbial ecology, evolution and metabolism, sequenced reference genomes of representative native prokaryotes are crucial. (biomedcentral.com)
  • In this study, we analyzed all genes from sequenced plastid genomes to unearth any neglected cases of HGT and to obtain a measure of the overall extent of HGT to the plastid. (biomedcentral.com)
  • The most widely understood role of RNA is in protein synthesis, which includes messenger RNA (mRNA), transfer RNA (tRNA) and ribosomal RNA (rRNA) (see Chapter 2). (printerresource.com)
  • Base modifications occur primarily in tRNA and ribosomal RNA (rRNA), and occur after the RNA is transcribed. (printerresource.com)
  • In bacteria , a single RNA polymerase produces the primary transcript precursors for all three major classes of RNA: messenger RNA (mRNA), ribosomal RNA (rRNA), and transfer RNA (tRNA). (basicmedicalkey.com)
  • Certain endogenous proteins, codon context, and standard amino acid concentrations can affect the yield of recombinant protein, the rate of nsAA incorporation within off-target proteins, and the rate of misincorporation due to near-cognate suppression or misacylation of orthogonal tRNA with standard amino acids. (bvsalud.org)
  • Transfer RNA (tRNA) modifications play a crucial role in maintaining proteostasis, but their impact in AD remains unclear. (bvsalud.org)
  • Additionally, exposing SH-WT cells to the secretome of SH-SWE cells led to reduced ELP3 expression, wobble uridine tRNA hypomodification, and increased protein aggregation. (bvsalud.org)
  • Among the conspicuous characteristics featuring its hyperthermophilic adaptation are overrepresentation of purine bases in protein coding sequences, higher GC-content in tRNA/rRNA sequences, distinct synonymous codon usage, enhanced usage of aromatic and positively charged residues, and decreased frequencies of polar uncharged residues, as compared to those in mesophilic organisms. (biomedcentral.com)
  • The initiator tRNA also contains conserved nucleotides that are recognized by proteins called eukaryotic initiation factors, or eIFs. (jove.com)
  • Together with eIF2 and GTP, the initiator tRNA binds the P site of the small ribosomal subunit forming the eukaryotic pre-initiation complex. (jove.com)
  • Now, a new tRNA, carrying the second amino acid, can bind to the A-site on the ribosome and protein synthesis can begin. (jove.com)
  • Initiator tRNA, ribosomal subunits, and eukaryotic initiation factors (eIFs) are all required to assemble on the initiation codon of mRNA. (jove.com)
  • To obtain a better understanding of metabolically active microbial communities, we tested a molecular ecological approach using poly(A) tailing of environmental 16S rRNA, followed by full-length complementary DNA (cDNA) synthesis and sequencing to eliminate potential biases caused by mismatching of polymerase chain reaction (PCR) primer sequences. (frontiersin.org)
  • The four archaeal 16S rRNA sequences that were obtained using the poly(A) tailing method formed deeply branching lineages that were related to Candidatus "Parvarchaeum" and the ancient archaeal group. (frontiersin.org)
  • Considering the current limitations of NGS technology, full-length 16S rRNA gene sequences are better suited to downstream analytical methods such as fluorescence in situ hybridization. (frontiersin.org)
  • A. Ribosomal RNA analysis StructRNAfinder - predicts and annotates RNA families in transcript or genome sequences. (printerresource.com)
  • Specific sequences on DNA, called promoters , determine where the RNA polymerase binds and how frequently it initiates transcription. (basicmedicalkey.com)
  • In a structural gene, the transcribed region contains the coding sequences that dictate the amino acid sequence of a polypeptide chain. (basicmedicalkey.com)
  • Exons, which contain the coding sequences for the proteins, are separated in pre-mRNA by introns , regions that have no coding function. (basicmedicalkey.com)
  • since the symptoms were suggestive of phytoplasma infection, plants were assayed for presence of phytoplasma by pcr amplification of 16s rdna and ribosomal protein (rp) gene sequences. (liverpool.ac.uk)
  • primers designed from sequences of the gene encoding the elongation factor tu (tuf gene) of several culturable mollicutes amplified most of the tuf gene from phytoplasmas of the aster yellows, stolbur and x-disease groups. (liverpool.ac.uk)
  • The incorporation of nonstandard amino acids (nsAAs) within protein sequences has broadened the chemical functionalities available for use in the study, prevention, or treatment of disease. (bvsalud.org)
  • Pairwise comparison of 105 orthologous protein sequences shows a strong bias towards replacement of uncharged polar residues of mesophilic proteins by Lys/Arg, Tyr and some hydrophobic residues in their Nanoarchaeal orthologs. (biomedcentral.com)
  • Global distribution of nearly identical phage-encoded DNA sequences. (academicinfluence.com)
  • Environmental sequences of ten novel plastid lineages and structural innovations in plastid proteins confirm that plastids in apicomplexans and their relatives are widespread and share a common, photosynthetic origin. (elifesciences.org)
  • Our search relied primarily on phylogenetic analyses, but also involved scrutiny of each potential case (including generation of new gene sequences from phylogenetically relevant taxa) to rule out artifacts and various types of homoplasy. (biomedcentral.com)
  • The increased number of resulting protein sequences creates an opportunity to expand protein engineering, but also presents a challenge as many gene product molecular functions are poorly annotated. (gatech.edu)
  • The numerous modified nucleotides in eukaryotic ribosomal RNA. (printerresource.com)
  • Eukaryotic genes are transcribed in the nucleus by three different RNA polymerases , each principally responsible for one of the major classes of RNA. (basicmedicalkey.com)
  • The significant conflict between eukaryotic phylogenies inferred from MATX and SSU rDNA data represents strong evidence that MATX paralogues have undergone horizontal gene transfer across the tree of eukaryotes. (biomedcentral.com)
  • Multiple sequence alignments of MAT genes from a wide diversity of eukaryotes demonstrated a paralogue of MAT, named MATX, with distinctive features that are absent in all other eukaryotic MATs. (biomedcentral.com)
  • Most eukaryotic mRNAs are monocistronic, that is, they encode only a single protein. (jove.com)
  • The DNA strand that directs the sequence of nucleotides in the RNA by complementary base pairing is the template strand. (basicmedicalkey.com)
  • The genome of strain SM1901 T was 4648537 nucleotides long and encoded a variety of cold adaptation related genes, providing clues for better understanding the ecological adaptation mechanisms of polar bacteria. (microbiologyresearch.org)
  • The genetic information of life on Earth have been conserved in natural nucleotides-A, G, C, T(U) forming two exclusive sets of base pairs A-T(U) & G-C, that complementing to the fundamental processes of DNA replication & RNA transcription by several polymerases and translation to functional proteins via ribosomal reactions. (fortuneonline.org)
  • Through a clever multi-omics design, the authors were able to use three major types of integrated analyses to identify differences in encoded and expressed microbial functions that were involved in the BPA-degrading microbial community. (rsc.org)
  • Bioinformatic analyses were performed to classify the Mhp field samples based on their similarity according to the presence of the studied genes. (biomedcentral.com)
  • This work provided insight into the diversity of Mhp field strains based on 16 genes using PCR assay and bioinformatic analyses. (biomedcentral.com)
  • Sequencing of the RNA via classical RNA-seq and subsequent specialised bioinformatic analyses the result in the prediction of inter- and intramolecular RNA-RNA interactions. (uni-stuttgart.de)
  • Upregulation of these genes in the deletion strain was confirmed by northern blot and proteome analyses. (uni-stuttgart.de)
  • The biological system is complex with many regulatory features such as DNA, mRNA, proteins, metabolites, and epigenetic features such as DNA methylation and histone post-translational modifications (PTMs). (rsc.org)
  • Because bacteria do not contain nuclei, ribosomes bind to mRNA as it is being transcribed, and protein synthesis occurs simultaneously with transcription. (basicmedicalkey.com)
  • This complex recognizes the mRNA by interacting with initiation factors eIF4E bound to the 5' cap, and eIF4G bound to the poly(A) tail-binding proteins. (jove.com)
  • Instead, each bacterial mRNA contains a leader sequence upstream of the first AUG codon, called the Shine-Dalgarno sequence. (jove.com)
  • It is encoded by the SSU- rDNA. (printerresource.com)
  • rRNA genes contain regions of variable DNA sequence that are unique to the species carrying the … Ribosomal RNA is transcribed from ribosomal DNA (rDNA) and then bound to ribosomal proteins to form small and large ribosome subunits. (printerresource.com)
  • They are different from mutations that give bacteria resistance to antibiotics by preventing the antibiotic from getting in or changing the shape of the target protein. (wikipedia.org)
  • They can also spread easily as they contain genes for constructing pili, which allow them to transfer the R-factor to other bacteria. (wikipedia.org)
  • R-factors have contributed to the growing antibiotic resistance crisis because they quickly spread resistance genes among bacteria. (wikipedia.org)
  • Genes in the resistance plasmids enable bacteria to produce Pilli and develop resistance to antibiotics. (wikipedia.org)
  • MDR genes in bacteria are transmitted mainly through the resistance plasmids. (wikipedia.org)
  • Distel, D. L., DeLong, E. F., and Waterbury, J. Epub … Bacterial Identification by 16S rRNA Gene Sequence Analysis Background All bacteria contain 16S ribosomal RNA (rRNA) genes of approximately 1500 base pairs (bp) in length. (printerresource.com)
  • 6. I want to study ribosomal RNA expression and potential processing sites in bacteria. (printerresource.com)
  • A., 1992, The use of 16S ribosomal RNA targeted oligonucleotide probes to study competition between ruminai fibrolytic bacteria. (printerresource.com)
  • The sheer number of non-human genes represented by the human microbiota - there are millions in our "extended genome" 2) compared to the nearly 23,000 in the human genome - implies we have just begun to fathom the full extent to which bacteria work to facilitate their own survival. (mpkb.org)
  • based methods for culturing bacteria have drastically underrepresented the size and diversity of bacterial populations. (mpkb.org)
  • 9) According to Asher Mullard, "Between them [the bacteria in our bodies], they harbor millions of genes, compared with the paltry 20,000 estimated in the human genome. (mpkb.org)
  • However, in obligatory intracellular bacteria, many genes involved in DNA recombination and repair along with the biosynthetic and metabolic genes are usually lost [ 3 , 4 ]. (biomedcentral.com)
  • Two other major roles for noncoding RNA are in genome defense and in genetic regulation. (printerresource.com)
  • Using an RNA genome, many viruses are known to encode their genetic information. (printerresource.com)
  • Genes in the human microbiota far outnumber those in the human genome. (mpkb.org)
  • Gene and Genetics are the main areas of his Genome studies. (research.com)
  • This is critical because, while individual genes or genome fragments provide useful information on the metabolic potential of a community, in practice most biochemical transformations take place inside a cell, involving sets of genes structured in controlled pathways. (biomedcentral.com)
  • Therefore, in this study, to select P. odoriferum -specific genes, species-specific genes were selected using the data of the P. odoriferum JK2.1 whole genome and similar bacterial species registered with NCBI. (ppjonline.org)
  • The mt genome of S. japonicus is a closed-circular and double-stranded DNA molecule of 16,274 bp (GenBank: MK341530), which consists of 13 protein-coding genes (PCGs), 2 rRNAs, 22 tRNAs and a putative control region (CR). (bvsalud.org)
  • Most, if not all of these transfers seem to be the result of a gene being transferred from the mitochondrial genome of one species to that of another. (biomedcentral.com)
  • While there are reasonable approaches to map RNA secondary structures genome-wide, understanding how different RNAs interact to carry out their regulatory functions requires mapping of intermolecular base pairs. (uni-stuttgart.de)
  • Genes are transcribed by enzymes called RNA polymerases that generate a single-stranded RNA identical in sequence (with the exception of U in place of T) to one of the strands of the double-stranded DNA. (basicmedicalkey.com)
  • P22 has strong 16S rRNA gene sequence similarity to Paenibacillus humicus (99.5%) ( 7 ). (asm.org)
  • sequence and rflp analysis of the elongation factor tu gene used in differentiation and classification of phytoplasmas. (liverpool.ac.uk)
  • This consensus AGGAGGU sequence serves as the ribosomal binding site by base pairing with a complementary sequence on the 16S rRNA of the small ribosomal subunit. (jove.com)
  • The c. 18-kb spinosyn genes in plasmid pUCAmT-spn served as the homologous sequence and guided a single-crossover homologous recombination to generate stable, apramycin-resistant exconjugants with all the genes duplicated. (mirnamimic.com)
  • A critical component of employing ML for protein engineering is the development of a model that predicts the fitness of a protein given its sequence. (gatech.edu)
  • During Spring of 2023 I found that using a Multilayer Perceptron taking as input a PLM embedding of a mutated amino acid with a protein sequence can accurately predict protein function (Fig. 1). (gatech.edu)
  • Recent multi-omics studies have revealed the heterogeneity and complexity of tumor features such as their genetic mutations, transcriptome, proteins, and signaling pathways. (rsc.org)
  • This straightforward technique is particularly suitable for large DNA molecules and is therefore ideal for engineering PKS and non-ribosomal peptide synthetase pathways. (mirnamimic.com)
  • 7) The human gut alone contains on average: 40,000 bacterial species, 8) 9 million unique bacterial genes and 100 trillion microbial cells. (mpkb.org)
  • We identified novel species-specific genes to detect and quantify targeted P. odoriferum and designed specific primer sets targeting HAD family hydrolases. (ppjonline.org)
  • Advancements in DNA sequencing technologies, particularly next-generation sequencing, have accelerated the discovery of numerous genes from an extensive variety of species. (gatech.edu)
  • 7 ] undertook the first metagenome-based community characterization on amplified 16S rRNA genes. (biomedcentral.com)
  • Here, we describe common methods applicable to nsAA incorporation within proteins in E. coli for sufficient purity and characterization for downstream therapeutic applications. (bvsalud.org)
  • We selected a small RNA (s479) from H. volcanii for detailed characterization. (uni-stuttgart.de)
  • RNA polymerases differ from DNA polymerases in that they can initiate the synthesis of new strands in the absence of a primer. (basicmedicalkey.com)
  • Although oceanic ammonia and nitrite oxidation are balanced, ammonia-oxidizing archaea (AOA) vastly outnumber the main nitrite oxidizers, the bacterial Nitrospinae. (nature.com)
  • article{marchfelder2021small, abstract = {The function and mode of action of small regulatory RNAs is currently still understudied in archaea. (uni-stuttgart.de)
  • According to a recent National Institutes of Health (NIH) estimate, 90% of cells in the human body are bacterial, fungal, or otherwise non-human. (mpkb.org)
  • RNA polymerase III promoters differ significantly from RNA polymerase II promoters in that they are located downstream from the transcription start site and within the transcribed segment of the DNA. (printerresource.com)
  • A gene is a segment of DNA that functions as a unit to generate an RNA product or, through the processes of transcription and translation, a polypeptide chain. (basicmedicalkey.com)
  • Here, we demonstrate that the transcription factor E2F6, a member of the polycomb repressive complex 1.6 (PRC1.6), is critical to target and initiate epigenetic silencing at germline genes in early embryogenesis. (cnrs.fr)
  • The involvement of RNA in such fundamental processes as protein synthesis and RNA processing has led to the idea that ribozymes were more common in early life. (printerresource.com)
  • These modifications are essential for their proper function in protein translation (see Chapter 13: Protein Synthesis). (printerresource.com)
  • The transcribed region of a gene contains the template for synthesis of an RNA, which begins at the start point. (basicmedicalkey.com)
  • In addition to the host regulatory mechanisms response to disease, the microbiome can make changes to the expression of the host features such as their genes, proteins, and/or PTMs. (rsc.org)
  • Such subcellular spatial transcriptomics (henceforth "SST") technologies provide not only the transcript abundance of each gene in a cell, but they also provide the precise subcellular locations of those transcripts, thus materializing a true spatial map of the transcriptome. (gatech.edu)
  • The Giardia lamblia vsp gene repertoire: characteristics, genomic organization, and evolution. (mbl.edu)
  • 2002). A large increase in nikkomycin production was obtained when an extra nikkomycin biosynthetic gene cluster was integrated into the genomic of Saccharopolyspora ansochromogenes (Liao et al. (mirnamimic.com)
  • In our study, the strategy of direct cloning based on Red/ET technology was applied to obtain the spinosyn biosynthetic gene cluster from the genomic DNA of S. spinosa, which is simple and convenient. (mirnamimic.com)
  • They are frequently accompanied by the genes encoding virulence determinants, specific enzymes or resistance to toxic heavy metals. (wikipedia.org)
  • This work demonstrated the genetic diversity of several Mhp field strains based on 16 selected genes related to virulence and/or immune response in Brazil. (biomedcentral.com)
  • Our findings demonstrate the difference between Mhp field strains could influence the virulence, and we hypothesize that the most frequent genes in Mhp field strains could possibly be used as vaccine candidates. (biomedcentral.com)
  • 30 kb) due to the considerable size of DNA required to encode the conjugation mechanisms that allow for cell-to-cell conjugation. (wikipedia.org)
  • We analyzed the genetic profile of Mhp field samples using 16 genes that encode proteins involved in the mechanisms of bacterial pathogenesis and/or the immune responses of the host. (biomedcentral.com)
  • Our findings elucidate the mechanisms of epigenetic targeting of germline genes and provide a paradigm for how transient repression signals by DNA-binding factors in early embryonic cells are translated into long-term epigenetic silencing during mouse development. (cnrs.fr)
  • A single new case, a bacterial rpl36 gene transferred into the ancestor of the cryptophyte and haptophyte plastids, appears to be a true HGT event. (biomedcentral.com)
  • Interestingly, this rpl36 gene is a distantly related paralog of the rpl36 type found in other plastids and most eubacteria. (biomedcentral.com)
  • The plastid acquisition of a bacterial rpl36 gene via HGT provides the first strong evidence for a sister-group relationship between haptophyte and cryptophyte plastids to the exclusion of heterokont and alveolate plastids. (biomedcentral.com)
  • If these are obtained from isolates, the encoded information can be complemented by phenotypic assays and ecophysiological response experiments to provide insights into the factors that regulate the activity of these populations, in particular biogeochemical processes. (biomedcentral.com)
  • The spinosyn-producing microorganism, S. spinosa, has been shown to be recalcitrant to genetic manipulation and gene transfer processes (Matsushima et al. (mirnamimic.com)
  • article{Schafer:2021, abstract = {RNA-RNA inter- and intramolecular interactions are fundamental for numerous biological processes. (uni-stuttgart.de)
  • To understand these processes, we need access to the spatial information of gene expression within tissues. (gatech.edu)
  • Thereafter, the specificity of the selected gene was tested through blast analysis. (ppjonline.org)
  • Plasmid-mediated resistance is the transfer of antibiotic resistance genes which are carried on plasmids. (wikipedia.org)
  • It is very common for the resistance genes or entire resistance cassettes to be re-arranged on the same plasmid or be moved to a different plasmid or chromosome by means of recombination systems. (wikipedia.org)
  • Most of the resistance plasmids are conjugative, meaning that they encode all the needed components for the transfer of the plasmid to another bacterium, and that isn't present in mobilizable plasmids. (wikipedia.org)
  • This plasmid shares many genes with the F factor and is largely homologous to it. (wikipedia.org)
  • Therefore, we only amplified the pUC replication origin, apramycin resistance gene, and oriT of RK2 from this plasmid as the linear cloning vector. (mirnamimic.com)
  • they play a role in the autonomous replication, conjugation, and ampicillin resistance genes. (wikipedia.org)
  • This suggests that gene replacement somehow occurred by recombination at the very ends of rpl36 , without the level and length of similarity normally expected to support recombination. (biomedcentral.com)
  • This phylogenetic pattern could have arisen by either differential loss of old paralogues or the spread of one of these paralogues by horizontal gene transfer. (biomedcentral.com)
  • Our results suggest that MATX entered the euglenid lineage in a single horizontal gene transfer event that took place after the secondary endosymbiotic origin of the euglenid chloroplast. (biomedcentral.com)
  • The obvious next step is to integrate this information and learn how genes, proteins, and/or epigenetic factors influence the phenotype of a disease in context of the system. (rsc.org)
  • about 85% of the tuf gene from two aster yellows strains and a tomato stolbur phytoplasma was sequenced. (liverpool.ac.uk)
  • Based on our results, we suspect that Mhp genetic variability may be associated with the frequency of genes among the field strains and we have demonstrated that some Mhp field samples could not have many important genes described in the literature. (biomedcentral.com)
  • Interestingly, s479 is not only encoded next to CRISPR-cas genes but the mature s479 contains a crRNA-like 5´ handle and experiments with Cas protein deletion strains indicate maturation by Cas6 and interaction with Cas proteins. (uni-stuttgart.de)
  • In this review, we discuss considerations of the study design for each data feature, the limitations in gene and protein abundance and their rate of expression, the current data integration methods, and microbiome influences on gene and protein expression. (rsc.org)
  • The traits potentially attributable to the symbiotic/parasitic life-style of the organism include the presence of apparently weak translational selection in synonymous codon usage and a marked heterogeneity in membrane-associated proteins, which may be important for N. equitans to interact with the host and hence, may help the organism to adapt to the strictly host-associated life style. (biomedcentral.com)
  • Upon codon-anticodon recognition, GTP is hydrolyzed and the initiation factors dissociate, allowing the large ribosomal subunit to join the complex and form an intact ribosome. (jove.com)
  • Common to all is the Psoralen-mediated in vivo RNA crosslinking followed by RNA Proximity Ligation to join the two interacting RNA strands. (uni-stuttgart.de)
  • Recently, different strategies to detect RNA-RNA duplexes in living cells, so called direct duplex detection (DDD) methods, have been developed. (uni-stuttgart.de)
  • Duplication of biosynthetic genes to increase the yield of corresponding secondary metabolite is a practicable and successful approach. (mirnamimic.com)
  • the biosynthetic gene cluster, which is time- Wnt assay and labor-consuming. (mirnamimic.com)
  • Microbes form most of the world's biomass: there are typically 40 million bacterial cells in a gram of soil and a million bacterial cells in a milliliter (gram) of fresh water. (mpkb.org)
  • Now, the 50S ribosomal subunit can bind to the initiation complex, with the complete ribosome ready to begin translation. (jove.com)
  • tomato flower abnormalities induced by stolbur phytoplasma infection are associated with changes of expression of floral development genes. (liverpool.ac.uk)
  • Other candidates that might play an auxiliary role in desilencing PY include TraY, which autoregulates its expression at the PY promoter (Silverman & Sholl, 1996), or another nucleoid-associated protein such as Lrp (Starcic-Erjavec et al. (mirnamimic.com)
  • With the ability to profile gene expression at cellular resolution, we are able to significantly increase our understanding of cell types and cell states within different complex biological systems. (gatech.edu)
  • The data generated from spatial transcriptomics experiments typically consists of gene expression levels associated with specific spatial coordinates. (gatech.edu)
  • The origin of the MATX paralogue is unclear, and it cannot be excluded that it arose by a gene duplication event before the most recent common ancestor of eukaryotes. (biomedcentral.com)
  • To such effect, I want to perform an RNA-Seq experiment in order to analyze potential rRNA transcripts isoforms and processing sites. (printerresource.com)
  • The primary transcripts are modified and trimmed to produce the mature RNAs. (basicmedicalkey.com)
  • Positively charged amino acid residues are relatively abundant in the encoded gene-products of N. equitans and other hyperthermophiles, which is reflected in their isoelectric point distribution. (biomedcentral.com)
  • The review also outlines the vast applications of UBPs as novel information storage components as well as in creation of semi-synthetic organisms expressing non canonical amino acids, high affinity aptamer generation, PCR based diagnostics and sitespecific labelling of RNAs. (fortuneonline.org)
  • His studies deal with areas such as Prokaryote, Clade, Phylogenetic tree and Genomics as well as Evolutionary biology. (research.com)
  • Our results showed variations in the frequency of the 16 studied genes among different Mhp field samples. (biomedcentral.com)
  • The ability to genetically encode the introduction of nsAAs at precise sites of target recombinant proteins has enabled numerous applications such as bioorthogonal conjugation, thrombin inhibition, intrinsic biological containment of live organisms, and immunochemical termination of self-tolerance. (bvsalud.org)
  • Protein language models (PLM) have been found to generate state-of-the-art representations of biological properties and achieve impressive prediction performance in protein prediction related tasks [4]. (gatech.edu)
  • In our work, we suggest the use of a PCR assay associated with bioinformatic tools for the analysis of genetic differences based on several Mhp genes. (biomedcentral.com)