• The mechanisms involve the proteins and DNA sequences inside cells. (wikipedia.org)
  • Recent breakthroughs have uncovered more and more DNA replication licensing machinery proteins (ORC, Cdc6, Cdt1, geminin, etc.) functioning in other cell cycle events, including centrosome replication, mitotic events, transcription and so on. (intechopen.com)
  • DNA replication origins retain mobile licensing proteins. (yeastgenome.org)
  • E proteins made by the viral genome promote the activation of host DNA replication mechanisms that can then be used by the virus during its own replication. (medscape.com)
  • These data suggest that the core complex ensures a stable platform for the C-terminal domain of Psf1 to act as a key interaction interface for other proteins in the replication-initiation process. (rcsb.org)
  • DDX5 is required for cell proliferation by controlling the transcription of genes expressing DNA replication proteins in cancer cells in which the DDX5 locus is amplified, and this has uncovered a dependence on DDX5 for cell proliferation. (aacrjournals.org)
  • Cell cycle progression, phosphorylation, and DNA binding of cell cycle checkpoint proteins were analyzed. (nih.gov)
  • By understanding the control of the fidelity of DNA replication in normal cells we aim to understand why defects in the proteins involved are associated with cancer. (sheffield.ac.uk)
  • Whenever genomic lesions are detected during DNA synthesis, they are coated by the single strand DNA binding protein complex, called Replication Protein A, leading to the activation of canonical checkpoint proteins like ATR, Chk1 and p53. (nii.res.in)
  • Human genome codes for many other single strand DNA binding proteins and we are testing if novel mechanisms of checkpoint activation exist that do not require Replication Protein A. If such mechanisms exist we would like to understand which factors provide the role of DNA binding, how checkpoint proteins like Chk1 and p53 are activated and how signal transduction pathway stalls the cell cycle and DNA replication. (nii.res.in)
  • It has been observed that some replication proteins localize to the centrosome suggesting their role in regulation of centrosome biogenesis. (nii.res.in)
  • Replication proceeds bi-directionally from a minority of potential origins licensed for replication by a variety of replication factor proteins. (lancs.ac.uk)
  • Our group has identified DNA topoisomerase IIα (Topo IIα) as one of the important mitotic proteins for SUMOylation. (ku.edu)
  • For about a decade, our group has been extending its field of research to the study and modeling of the structure and dynamics of biological molecules (DNA, proteins). (ens-lyon.fr)
  • These LRC are the signature of the DNA-histone proteins interactions within the nucleosomes, the basic units for DNA compaction in eukaryotic cell nuclei, that constitute a regulatory factor for accessibility to genetic material. (ens-lyon.fr)
  • Have you ever wondered how DNA and proteins work together to carry out the functions of life? (freescience.info)
  • It unravels the secrets of genes, which are segments of genetic material, such as DNA, containing instructions for building proteins and controlling various cellular processes. (freescience.info)
  • By investigating nucleic acids like DNA and RNA, as well as proteins and other biomolecules, scientists gain insights into gene expression, protein synthesis, gene transfer, and more. (freescience.info)
  • The protein p53 is one of the most important tumor suppressor proteins: its activation can lead to cell cycle arrest, DNA repair, or apoptosis. (biomedcentral.com)
  • We find that this type of non-canonical fork convergence in fission yeast is prone to trigger deletions between repetitive DNA sequences via a mechanism we call Inter-Fork Strand Annealing (IFSA) that depends on the recombination proteins Rad52, Exo1 and Mus81, and is countered by the FANCM-related DNA helicase Fml1. (elifesciences.org)
  • For instance, SMC proteins (COOH-terminal fragments) are capable of binding DNA in the absence of Mcd1p. (rupress.org)
  • These studies furthered understanding of specific organ and tissue sites of virus replication at different phases of disease progression and were thus considered useful in studies designed to assess the efficacy of antiviral drugs. (who.int)
  • Are longitudinal changes in circulating tumor DNA (ctDNA) methylation effective in monitoring disease progression from molecular residual disease to recurrence? (cdc.gov)
  • Previous studies by us and others have suggested that differences in disease progression rate may be associated with both viral parameters, such as diversity or infecting subtype and host immune responses. (lu.se)
  • The detailed underlying mechanisms that determine differences in disease progression and clinical presentation of acute HIV infection (AHI) are largely unknown. (lu.se)
  • Interestingly, the disease progression rate can vary considerably between individuals (with time to AIDS ranging between months to decades), and the immune specificity against HIV infection has been suggested to only explain about 22% of the most reliable predictor of when AIDS will develop? (lu.se)
  • Importantly, this implies that up to 78% of the variation in HIV disease progression rate between patients remains to be explained. (lu.se)
  • RNA:DNA hybrids such as R-loops affect genome integrity and DNA replication fork progression. (biorxiv.org)
  • Here we find that the replication fork progresses at 1.3kbp/min in mouse fibroblast cells, consistent with other studies, and quantify replication fork stalling by replication inhibitor aphidicolin. (lancs.ac.uk)
  • Around these putative origins, genes are abundant and broadly expressed, and their transcription is co-oriented with replication fork progression. (ens-lyon.fr)
  • Our data reveal that histone acetylation level affects DNA replication dynamics of the sex chromosomes' heterochromatin and leads to a global reduction in replication fork rate genome wide. (biomedcentral.com)
  • Replication fork barriers are a commonly encountered problem, which can cause fork collapse and act as hotspots for replication termination. (elifesciences.org)
  • However, replication restart is relatively slow and, therefore, replication termination may frequently occur by an active fork converging on a collapsed fork. (elifesciences.org)
  • Regardless of its precise form, collapse renders the fork incompetent for further DNA synthesis. (elifesciences.org)
  • RDR can initiate from a collapsed fork where the DNA is either broken or remains intact. (elifesciences.org)
  • Cenu obdržel například Pavel Moudrý z Ústavu molekulární a translační medicíny za práci High speed of fork progression induces DNA replication stress and genomic instability, která vyšla v časopisu Nature, nebo Vladimír Divoký a Monika Horváthová z Ústavu biologie za příspěvky v časopisu Blood. (upol.cz)
  • PCNA is required for the coordinated synthesis of both leading and lagging strands at the replication fork during DNA replication. (bvsalud.org)
  • Epidemiologic, animal, and laboratory studies suggest that 5-amino-salicylic acid (5-ASA) protects from the development of CRC by altering cell cycle progression and by inducing apoptosis. (nih.gov)
  • The reversible lysine acetylation of histone H3 may play a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication and repair, nuclear import and neuronal repression. (abcam.com)
  • I characterised the cellular effects of BET protein inhibition in pre-B ALL and showed this was associated with inhibition of cell cycle progression, downregulation of c-Myc protein, direct inhibition of DNA replication and induction of caspase-dependent apoptosis that was independent of p53 activity. (bham.ac.uk)
  • E2F1 (UniprotKB: Q01094), belongs to the E2F family of transcription factor (TF), has a central role in regulating cell cycle progression and apoptosis [ 11 , 12 ] and, therefore, E2F1 activity is tightly governed by multiple feedback mechanisms and by the tumour suppressor Rb. (biomedcentral.com)
  • In this chapter, we mainly discuss the coordination regulations between DNA replication initiation and other cell cycle events that ensure genomic integrity. (intechopen.com)
  • DNA replication occurs once and only once per cell cycle mainly regulated by DNA replication initiation factors in eukaryotic cells. (intechopen.com)
  • The pre-replication complex (pre-RC) assembly or the DNA replication licensing is the first step in DNA replication initiation, characterized by the sequential recruitment of ORCs, Cdc6, Cdt1 and MCMs to the DNA replication origins to form the pre-RC at the end of mitosis ( Bell and Dutta 2002 ). (intechopen.com)
  • DDK regulates replication initiation by controlling the multiplicity of Cdc45-GINS binding to Mcm2-7. (yeastgenome.org)
  • Bryant JA and Aves SJ (2011) Initiation of DNA replication: functional and evolutionary aspects. (yeastgenome.org)
  • It is known that replication factors are downregulated during stress and we have observed that cullin 4-based ubiquitin ligase mediate the proteolysis of Mcm10, a protein essential for the initiation and elongation phases of DNA replication. (nii.res.in)
  • Here using an in vitro cell-free DNA replication system we analyse the interplay between Ciz1 and cyclin A/CDK2 in regulation of the initiation phase of DNA replication. (lancs.ac.uk)
  • These data suggest that Ap4A inhibits initiation by reducing loading of the replicative helicase MCM2-7 and the DNA polymerase sliding clamp PCNA. (lancs.ac.uk)
  • We have proposed that this specific organization could result from the constraints of accommodating the replication and transcription initiation processes at chromatin level, and reducing head-on collisions between the two machineries. (ens-lyon.fr)
  • The eukaryotic GINS complex is essential for the establishment of DNA replication forks and replisome progression. (rcsb.org)
  • Completion of genome duplication is challenged by structural and topological barriers that impede progression of replication forks. (ku.dk)
  • Previously it was thought that DNA replication began at an origin in chromosomal DNA adjacent to the telomere repeats, with the replication forks moving bidirectionally away from the subtelomeric origin ( Fig. 1 A ), thus replicating the telomere. (rupress.org)
  • Collapsed forks can be rescued by homologous recombination, which restarts replication. (elifesciences.org)
  • Eukaryotic DNA replication initiates at multiple origin sites along each chromosome and terminates when replication forks (RFs) from adjacent origins converge. (elifesciences.org)
  • Recombination and Pol ζ Rescue Defective DNA Replication upon Impaired CMG Helicase-Pol ε Interaction. (yeastgenome.org)
  • We show that the number of such 53BP1 nuclear bodies increases after genetic ablation of BLM, a DNA helicase associated with dissolution of entangled DNA. (ku.dk)
  • E1 protein has helicase activity for replication, and E2 encodes DNA-binding protein for regulation of transcription. (medscape.com)
  • Certain mechanisms are present to prevent cells from indefinite division, which is mostly done by programmed failure in DNA synthesis. (wikipedia.org)
  • Indeed, a number of agents currently used in cancer treatment are known to target DNA synthesis. (aacrjournals.org)
  • Replication is catalysed by processive replication enzymes known as DNA polymerases and is limited to the synthesis phase (S phase) of the cell cycle. (lancs.ac.uk)
  • Folate functions as a coenzyme or cosubstrate in single-carbon transfers in the synthesis of nucleic acids (DNA and RNA) and metabolism of amino acids [ 1-3 ]. (nih.gov)
  • Eukaryotic chromosomes are duplicated via semiconservative replication with a leading (continuous synthesis for net growth at the 3′ end of the nascent leading strand) and lagging (discontinuous Okazaki fragment synthesis for net growth at the 5′ end of the nascent lagging strand) elongating strand as shown in Fig. 1 . (rupress.org)
  • Nuclear antigen with a role in DNA synthesis, DNA repair, and cell cycle progression. (bvsalud.org)
  • Together, these data indicate that restoration of DNA or chromatin integrity at loci prone to replication problems requires mitotic transmission to the next cell generations. (ku.dk)
  • During the mitotic mammalian cell cycle cells faithfully replicate their DNA utilizing multiple DNA replication sites known as origins of replication. (lancs.ac.uk)
  • This so-called recombination-dependent replication (RDR) helps ensure that DNA is fully replicated prior to sister chromatid segregation, thereby avoiding mitotic catastrophes. (elifesciences.org)
  • Thus, the products of chromosome replication (sister chromatids) generated during S-phase must be identified over time until mitosis when sisters associate with the mitotic spindle and segregate away from each other into the newly forming daughter cells. (rupress.org)
  • All HPV subtypes contain early (E) genes and late (L) genes, which are essential to viral integration and replication. (medscape.com)
  • E genes are responsible for episomal replication. (medscape.com)
  • Emerging evidences suggest that microRNA target genes that regulate DNA replication and cell cycle progression and we aim to determine how microRNA regulate the DNA replication machinery as cell progresses from one phase to the next. (nii.res.in)
  • During the process, all cell duplication process, including mitosis, meiosis as well as DNA replication, will be paused. (wikipedia.org)
  • The permanent cell cycle withdrawal is mainly done by the wearing off of DNA sequences during S Phase, the second stage during a DNA replication progress. (wikipedia.org)
  • Dr. Shen's research has been focused on the roles for cell growth signaling pathways (including RAS-MAPK and PI3K-mTOR) in the regulation of cell cycle progression and DNA damage repair. (cityofhope.org)
  • The precise regulations of pre-RC protein levels and assembly are effective ways to prevent reassembly of de novo MCM2-7 onto the replicated origins to re-license and re-replicate the genomic DNA in the subsequent phases of the same cell cycle ( Figure 1) . (intechopen.com)
  • Defects in the control of cell proliferation are a hallmark of cancer, and DNA replication is a key process for cell proliferation. (aacrjournals.org)
  • In this study, we hypothesized that 5-ASA restrains cell cycle progression by activating checkpoint pathways in colorectal cell lines, which would prevent tumor development and improve genomic stability. (nih.gov)
  • We found that 5-ASA at concentrations between 10 and 40 mmol/L affects cell cycle progression by inducing cells to accumulate in the S phase. (nih.gov)
  • Beyond their nutritional impact, clear evidence is beginning to link modifications in chromatin structure induced by butyrate to cell cycle progression, DNA replication and overall chromosome stability. (usda.gov)
  • This protein was found to also interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggests a regulatory role in DNA replication during the S-phase of the cell cycle. (cancerindex.org)
  • DNA replication is a vital process of life and must be completed precisely during each cell cycle. (nii.res.in)
  • When mammalian cell experiences DNA damage, it activates checkpoint mechanisms to stall the progression of cell cycle and DNA replication. (nii.res.in)
  • Aberrations in microRNA activity has been linked to loss of cell cycle and replication control and this has led to oncogenic functions (thereby called oncomirs) being ascribed to them. (nii.res.in)
  • Summing up, we are trying to understand the mechanisms by which microRNAs regulate mammalian cell cycle and DNA replication in normal and pathological conditions. (nii.res.in)
  • These processes, referred to as DNA damage tolerance mechanisms, are required for cell proliferation and generate many of the mutations that contribute to genetic variation and cancer development. (mskcc.org)
  • The goal of the research is to first understand the basic logic of the DNA damage response, and then develop predictive quantitative models of the molecular processes that govern the dynamics of DNA replication, DNA damage tolerance, and cell cycle progression. (mskcc.org)
  • Callegari AJ, Kelly TJ, Coordination of DNA damage tolerance mechanisms with cell cycle progression in fission yeast. (mskcc.org)
  • DNA is replicated to provide each daughter cell a complete copy of the genome. (lancs.ac.uk)
  • Cell cycle progression is largely controlled by the activity of cyclin dependent kinases (CDKs) and their cyclin binding partners. (lancs.ac.uk)
  • This demonstrates that Ciz1 modulates and enhances the activity of cyclin A-CDK2 in cell free DNA replication assays and that Ciz1 increases the permissive CDK range that can promote DNA replication. (lancs.ac.uk)
  • Next the inhibitory effect of Ap4A in cell free DNA replication assays is studied. (lancs.ac.uk)
  • These data demonstrate the potential for cell free DNA replication assays to be combined with DNA combing to dissect replication parameters and characterise DNA replication stress in future studies. (lancs.ac.uk)
  • A post-translational modification with SUMO (SUMOylation) can regulate various cellular events such as DNA replication, repair, transcription and cell cycle regulation. (ku.edu)
  • Many studies have indicated that SUMOylation is crucial for proper cell cycle progression. (ku.edu)
  • During cell growth and proliferation, ubiquitin plays an outsized role in promoting progression through the cell cycle. (intechopen.com)
  • Progression through the cell cycle is driven by the oscillating activity of Cyclin Dependent Kinases (CDKs). (intechopen.com)
  • Another folate-dependent reaction, the methylation of deoxyuridylate to thymidylate in the formation of DNA, is required for proper cell division. (nih.gov)
  • During the infection process, bacteriophages can transfer foreign DNA to their host (including virulence factors), integrate into the host genome, and/or kill their host through cell lysis ( Chen and Novick, 2009 ). (frontiersin.org)
  • For instance, DNA replication and DNA damage checkpoints delay cell cycle progression until each chromosome is fully replicated and physically intact. (rupress.org)
  • Given the pivotal role that E2F1 has in cell proliferation, its involvement in melanoma growth and progression is not surprising. (biomedcentral.com)
  • Understanding the association between Cell-free DNA levels in embryo CM and the quality of embryo cleavage could help improve the quality of IVF techniques. (who.int)
  • This prospective study was conducted with 96 spent CM from patients undergoing IVF cycle, in order to determine relationships of Cell-free DNA levels in embryo CM with embryo cleavage quality on day 3. (who.int)
  • Day 2 and day 3 CM corresponding to each one of the embryos was analyzed, by quantitative PCR, for estimation of Cell-free DNA levels. (who.int)
  • The results revealed a significant increase in Cell-free DNA levels on day 2 CM corresponding to 4 to 6 cell embryos compared to those corresponding to 7 to 8 cel embryos (p=0.04). (who.int)
  • As for day 3 CM, the results showed no significant difference between the Cell-Free DNA levels in CM of 7-8 and those of 4-6 cell embryos (p=0.4). (who.int)
  • Embryo morphology al ows options, the discovery of cell-free DNA in the evaluation of its growth, viability, and biological fluids has led to major advances in implantation capacity. (who.int)
  • In conclusion, we mapped major epigenetic modifications controlling the structure of the sex chromosome-associated heterochromatin and demonstrated the occurrence of differences in the molecular mechanisms controlling the replication timing of the heterochromatic blocks at the sex chromosomes in female Microtus cabrerae cells. (biomedcentral.com)
  • The results revealed novel regulatory mechanisms involved in ccRCC progression, hoping that it may spark an insight for later discovery about the new therapeutic targets for ccRCC. (bvsalud.org)
  • We identify an important role for DDX5 in G 1 -S-phase progression where it directly regulates DNA replication factor expression by promoting the recruitment of RNA polymerase II to E2F-regulated gene promoters. (aacrjournals.org)
  • To test whether histone acetylation level regulates its replication dynamics, we induced either global hyperacetylation by pharmacological inhibition or by targeting a histone acetyltransferase to the heterochromatic region of the X chromosomes. (biomedcentral.com)
  • These included PTEN-dependent DNA damage repair in response to SHP2 inhibition, AKT-mediated bypass resistance in response to chronic SHP2 inhibition, and SHP2 control of hypoxia-inducible factor expression through multiple MAPKs. (aacrjournals.org)
  • A dimerized coiled-coil domain and an adjoining part of geminin interact with two sites on Cdt1 for replication inhibition. (nii.res.in)
  • HPV is now known to be a small deoxyribonucleic acid (DNA) virus that infects epithelial cells and causes a variety of skin lesions. (medscape.com)
  • Here, we show that mild replication stress increases the frequency of chromosomal lesions that are transmitted to daughter cells. (ku.dk)
  • During evolution, DNA transcription and replication induce some compositional asymmetry (skew) along the DNA sequences. (ens-lyon.fr)
  • In higher eukaryotes, the nuclear genome is compartmentalized into distinct chromatin territories to facilitate the regulation of complex processes such as DNA repair, transcription and replication. (biomedcentral.com)
  • Dukaj L and Rhind N (2021) The capacity of origins to load MCM establishes replication timing patterns. (yeastgenome.org)
  • Therefore, we are trying to understand the independent mechanism by which mammalian cells effectively inhibit the replication machinery during stress preventing it from drifting towards a catastrophic path of genomic instability. (nii.res.in)
  • Changes in the timing of replication, origin usage and replication rate are indicative of DNA replication stress, a proposed hallmark of cancer that causes genome instability. (lancs.ac.uk)
  • Exposure of lymphoblastoid cells to WTC particulate matter led to dysregulation of DNA replication at common fragile sites in vitro. (nature.com)
  • Yeast heterochromatin regulators Sir2 and Sir3 act directly at euchromatic DNA replication origins. (yeastgenome.org)
  • These data suggest that Ap4A can inhibit the firing of replication origins through disruption of replication complex assembly. (lancs.ac.uk)
  • Deploying a multi-scale strategy of sharp upwardjump detection in noisy skew profiles, we have identified more than 1000 putative master origins of replications that are central to the Human genome organization. (ens-lyon.fr)
  • These features weaken progressively with the distance from putative replication origins. (ens-lyon.fr)
  • Our findings has provided a new model of gene organization in the human genome, which integrates transcription, replication, and chromatin structure as coordinated determinants of genome architecture. (ens-lyon.fr)
  • The DNA structure in these organisms are in the form of circular chromosomes, meaning there would be no "ends" appearing in their DNA. (wikipedia.org)
  • When further modeling DNA as a semifexible polymer explicitly taking into account the structural disorder induced by the sequence, we have provided some evidence that the observed LRC favor the spontaneous formation as well as the cooperative positioning of nucleosomes along the chromosomes including the nucleosome free regions experimentally observed at gene promoters (see figure below). (ens-lyon.fr)
  • Euchromatin is replicated in early S-phase, when the replication machinery is present as a multitude of small replication foci that are well distributed throughout the nuclear interior. (biomedcentral.com)
  • Antígeno nuclear que juega un papel en la síntesis y reparación del ADN, y en la progresión del ciclo celular. (bvsalud.org)
  • Semiconservative replication occurs before the action of telomerase. (rupress.org)
  • The activation of replication checkpoint may slow down DNA replication and improve DNA replication fidelity, which increases the maintenance of genomic stability and counteracts carcinogenesis. (nih.gov)
  • A p53 dependent checkpoint pathway prevents re-replication. (nii.res.in)
  • Furthermore, we highlighted a conserved role of histone acetylation level on replication dynamics across mammalian species. (biomedcentral.com)
  • I study the molecular processes that allow cells to replicate areas of the genome that contain DNA damage. (mskcc.org)
  • Dr Cunliffe's group leverages a combination of genomic technologies, laboratory models, and patient clinical specimens to dissect and understand the molecular basis of disease onset and malignant progression. (otago.ac.nz)
  • Molecular biology aids in drug discovery by identifying potential therapeutic targets and evaluating their efficacy in DNA. (freescience.info)
  • Molecular biology techniques, such as DNA sequencing and genotyping, have facilitated the identification of genetic variations that impact drug metabolism and efficacy. (freescience.info)
  • Thanks to molecular biology techniques like DNA sequencing and gene expression analysis, biologists have made significant strides in understanding genetics and genomics. (freescience.info)
  • Molecular biology techniques are instrumental in designing safe and effective gene delivery systems for the manipulation of DNA and genome. (freescience.info)
  • Early Detection of Molecular Residual Disease and Risk Stratification for Stage I to III Colorectal Cancer via Circulating Tumor DNA Methylation. (cdc.gov)
  • Piberger AL, Bowry A, Kelly RDW, Walker AK, González-Acosta D, Bailey LJ, Doherty AJ, Méndez J, Morris JR, Bryant HE & Petermann E (2020) PrimPol-dependent single-stranded gap formation mediates homologous recombination at bulky DNA adducts . (sheffield.ac.uk)
  • Maintenance of genome integrity is one of the crucial functions in life, to preserve the appropriate genetic information, being homologous recombination a key process in the DNA repair. (uni-marburg.de)
  • Together with genetics, I have added a new level of complexity in the regulation of homologous recombination as a multiway process in which many factors are involved in different avenues with partially overlapping functions depending on the kind of DNA damage generated. (uni-marburg.de)
  • In response to DNA double strand breaks, serine at position 15 of the TP53 (p53) tumor suppressor protein is rapidly phosphorylated by the ATM kinase. (reactome.org)
  • In this cohort study of 299 patients with colorectal cancer, circulating tumor DNA status was evaluated with 6 DNA methylation markers. (cdc.gov)
  • He Z, Houghton PJ, Williams TM, Shen C* . Regulation of DNA duplication by the mTOR signaling pathway. (cityofhope.org)
  • Ultraviolet Radiation Stress Triggers the Down-regulation of Essential Replication Factor Mcm10. (nii.res.in)
  • The withdrawal process also prevents diseased cells, or cells with mutated or damaged DNA, from continuing to divide and increasing the percentage of abnormal cells inside the body. (wikipedia.org)
  • Inhibiting BRAF Oncogene-Mediated Radioresistance Effectively Radiosensitizes BRAFV600E-Mutant Thyroid Cancer Cells by Constraining DNA Double-Strand Break Repair. (cityofhope.org)
  • A Role of hIPI3 in DNA Replication Licensing in Human Cells. (yeastgenome.org)
  • We applied an assay that measures the stability of maintenance of an episomal plasmid in human tissue culture cells to screen for new DNA replication factors. (aacrjournals.org)
  • Understanding how DNA replication is regulated in human cells can provide insight into cancer development and may reveal vulnerabilities that can be exploited therapeutically. (aacrjournals.org)
  • Our previous results indicate that 5-ASA improves replication fidelity in colorectal cells, an effect that is active in reducing mutations. (nih.gov)
  • Halliwell JA, Gravells P & Bryant HE (2020) DNA fiber assay for the analysis of DNA replication progression in human pluripotent stem cells . (sheffield.ac.uk)
  • in cells lacking RnhC, DNA replication is disrupted leading to transversions and structural variation. (biorxiv.org)
  • Double-strand breaks in DNA can wreak havoc in cells if not repaired. (rupress.org)
  • The majority is due to 'bad luck,' that is, random mutations arising during DNA replication in normal, noncancerous stem cells. (cdc.gov)
  • In vivo and in organized cells, and proper symmetry are healthy individuals, macrophages can characteristics of higher-quality embryos, which phagocytize DNA that has been passively point to healthy development and higher rates of released into the blood from apoptotic or necrotic implantation. (who.int)
  • To survey the epigenetic landscape of human CMML, we interrogated mutational spectrum, transcription, DNA methylation, histone modifications, and chromatin accessibility in ASXL1 MT ( n = 8) and ASXL1 WT ( n = 8) CMML (Fig. 1a ). (nature.com)
  • Miller CLW and Winston F (2023) The conserved histone chaperone Spt6 is strongly required for DNA replication and genome stability. (yeastgenome.org)
  • Euchromatin includes less-condensed transcriptional active regions, determined by a depletion of methylated DNA, an enrichment in specifically methylated histones such as H3K4, H3K36, H3K79 and a high level of histone acetylation [ 13 ]. (biomedcentral.com)
  • Since it is well recognized that errors in the centrosome duplication contribute to tumorigenesis we are interested in determining the role of replication factors in preventing aneuploidy. (nii.res.in)
  • The identification of both replication factor C and DNA helicases as critical for sister chromatid pairing has brought new insights into this fundamental process. (rupress.org)
  • The DNA composition and transcriptional activity of these heterochromatin blocks have been studied, but little is known about their DNA replication dynamics and epigenetic composition. (biomedcentral.com)
  • Replication of HCV results were confirmed by third generation is accelerated in the presence of HIV (prob- immunoblot assay (Lia-Tek-III kit, Or- ably as a result of immunodeficiency): the ganon, Amsterdam). (who.int)
  • Serologic markers change over typical courses of resolved acute infection and progression to chronic infection ( Figure 1 ) ( 15 ). (cdc.gov)
  • Deletion of either rnhB or rnhC triggers RNA:DNA hybrid accumulation, but with distinct patterns of mutagenesis and hybrid accumulation. (biorxiv.org)
  • It is well known that the products of chromosome replication are paired to ensure that the sisters segregate away from each other during mitosis. (rupress.org)
  • A separate side to Dr Bryant's work is the development of novel transition metal-containing photodynamic therapy agents that bind to DNA and induce damage only upon irradiation with specific wavelengths of light. (sheffield.ac.uk)
  • Using pulse-chase replication labeling and time-lapse microscopy, we found that the heterochromatic block enriched for macroH2A/H3K27me3 of the X chromosome is replicated during mid-S-phase, prior to the heterochromatic block enriched for H3K9me3/HP1 beta, which is replicated during late S-phase. (biomedcentral.com)
  • The DNA replication process is highly regulated both spatially and temporally, resulting in the changing pattern of replication structures throughout S-phase. (biomedcentral.com)
  • This type of heterochromatin is replicated during the mid-S-phase, when the replication foci become larger and are located around the nucle(ol)ar periphery. (biomedcentral.com)
  • This prevents the normal progression from G1 to S phase, thus providing a check on replication of damaged DNA (Banin et al. (reactome.org)
  • Understanding factors required for DNA replication will enrich our knowledge of this important process and potentially identify vulnerabilities that can be exploited in cancer therapy. (aacrjournals.org)