• High expression of RAD18 facilitated a highly stem-cell phenotype through the Hippo/YAP pathway, which supports the proliferation of TNBC. (nature.com)
  • Although steady-state conditions revealed no increase in primitive cell proliferation in p21-null mice, a significantly larger fraction of quiescent neural precursors was activated in the hippocampus and subventricular zone after brain ischemia. (rupress.org)
  • Therefore, p21 is an intrinsic suppressor to neural regeneration after brain injury and may serve as a common molecular regulator restricting proliferation among stem cell pools from distinct tissue types. (rupress.org)
  • Enriched expression of genes in LRECb was associated with stem cell attributes and identified WNT, TGF-β, and MAPK pathways of self renewal and proliferation. (frontiersin.org)
  • During rapid mammary growth in the mouse, label retaining epithelial cells (LREC) appear to retain label by asymmetric distribution of DNA strands, as evidenced by a rapid proliferation index of the LREC ( Smith, 2005 ). (frontiersin.org)
  • During periods of low mammary proliferation, quiescence of the stem cell population may account for retention of label. (frontiersin.org)
  • Overall, our study demonstrates the coordinated expression of functionally related genes during proliferation and differentiation of rainbow trout adipocyte cells. (biomedcentral.com)
  • An aberrant miRNA expression could contribute to cancer development and progression [ 6 , 7 ] and could affect their target genes that are involved in many biological processes, such as cell differentiation, proliferation, apoptosis, metabolism, and development [ 8 ]. (hindawi.com)
  • WAT is characterised by its capacity to adapt and expand in response to surplus energy through processes of adipocyte hypertrophy and/or recruitment and proliferation of precursor cells in combination with vascular and extracellular matrix remodelling. (springer.com)
  • Cell viability/proliferation assays, cell cycle analysis by flow cytometry, immunodetection of specific histone and p53 acetylation marks, western blotting, siRNA and RT-qPCR. (biomedcentral.com)
  • Although treatment with curcumin, garcinol or the garcinol derivative LTK-14 hampered MCF7 cell proliferation, differential effects of these compounds on histone modifications were observed. (biomedcentral.com)
  • The proliferation of choroidal endothelial cells (CECs) is one important step in the formation of new vessels. (molvis.org)
  • Transcriptional coactivator Yes-associated protein (YAP) can promote the proliferation of multiple cancer cells, corneal endothelial cells, and vascular smooth muscle cells, which participate in angiogenesis. (molvis.org)
  • This study showed that YAP upregulation promoted CNV formation by upregulating the proliferation of endothelial cells, providing evidence for the molecular mechanisms of CNV and suggesting a novel molecular target for nAMD treatment. (molvis.org)
  • Among them, VEGF-A can promote the division and proliferation of vascular endothelial cells and neovascularization and maintain the survival of new vessels. (molvis.org)
  • Low power laser irradiation (LPLI) promotes proliferation of multiple cells, which (especially red and near infrared light) is mainly through the activation of mitochondrial respiratory chain and the initiation of cellular signaling. (biomedcentral.com)
  • Cell proliferation is a very important physiological effect for low power laser irradiation (LPLI) used in clinical practice. (biomedcentral.com)
  • However, the mechanisms of cell proliferation induced by LPLI are poorly understood. (biomedcentral.com)
  • Recently, a large number of signaling proteins reported play an important key role in the process of LPLI-induced cell proliferation, probably due to the fact that the molecular events they are involved in are the basic response of the cells to extracellular stimuli. (biomedcentral.com)
  • In response to mito- pendence between the key mecha- gens, cell proliferation is triggered by nistic characteristics. (who.int)
  • H - Ras cell proliferation are also linked with haematopoietic cel s. (who.int)
  • Whereas it seems intuitive that an immune cell type that depends on regular proliferation outbursts for function may have evolved to modulate telomerase expression it is less obvious why others may also do so, as has been suggested for macrophages and neutrophils in some chronic inflammation disease settings. (biomedcentral.com)
  • B-cells have also been described to up-regulate telomerase expression during maturation, a process that also relies on proliferation bursts [ 16 ]. (biomedcentral.com)
  • Very similar findings were seen in a prior study which figured ARID1B may be the preferential gene necessary for the success of in knockdown in cell lines led to reduced proliferation and decreased invasion [85,97]. (morainetownshipdems.org)
  • Early embryonic pancreas-specific removal of resulted in decreased multipotent pancreatic progenitor cell proliferation and led to pancreas hypoplasia [48], indicating its essential function in modulating gene appearance during AG-99 development. (morainetownshipdems.org)
  • These cells are the original precursors of various cells in a tumor, whereas other intratumoral cells are limited in terms of their potential for proliferation and pluripotent differentiation. (biomedcentral.com)
  • This process is mediated by 2 mutually exclusive programs of gene expression: 1) an undifferentiated program supporting proliferation by stem cells within the basal layer and 2) a differentiation program instructing growth arrest and differentiation-associated programmed cell death in suprabasal layers. (stanford.edu)
  • Our data reveal for the first time that the ingestion of indole-3-carbinol, as administered, diminishes proliferation and increases apoptosis of tumor cells in an experimental model of inflammatory breast cancer, although this effect could not be enough to avoid the appearance of tumor embolization and metastasis. (biomedcentral.com)
  • However, the possible differences in proliferation and differentiation capabilities among independently-derived human embryonic stem cells (hESCs) are not well known because of insufficient characterization. (biomedcentral.com)
  • Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E. Ep-CAM mediates Ca2+-independent homotypic interactions. (thermofisher.com)
  • At around the same time, it was determined that stem cells from the amniotic membrane also have multipotent potential. (wikipedia.org)
  • In 2007, it was confirmed that the amniotic fluid contains a heterogeneous mixture of multipotent cells after it was demonstrated that they were able to differentiate into cells from all three germ layers but they could not form teratomas following implantation into immunodeficient mice. (wikipedia.org)
  • Neurogenesis in the adult hippocampus is a highly regulated process that originates from multipotent progenitors in the subgranular zone (SGZ). (jneurosci.org)
  • OLs are the myelin-forming cells of the central nervous system and they derive from progenitor cells generated by multipotent precursors. (rupress.org)
  • The E3 ubiquitin ligase RAD18 is well known for the maintenance of genome stability and cell survival through many DNA damage response (DDR) pathways such as translesion DNA synthesis (TLS) and homologous recombination repair (HRR) [ 4 , 5 ]. (nature.com)
  • It is involved in p53 pathways and is implicated in cell death/survival signaling, the cell cycle, and differentiation, thereby playing a regulatory role in carcinogenesis [ 12 ]. (hindawi.com)
  • In addition, miRNA profiling shows enrichment of miRNA-targeted genes in retinoid receptors and PPARGC1α / PPARγ signaling, and antigen presentation pathways. (nature.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • The regulation of the stem cell-like properties by several important signaling pathways have been found to endow the tumor cells with an increased level of tumorigenicity, chemoresistance, and metastatic ability. (molcells.org)
  • AS their differentiation into neural and glial cells as well as hepatocyte precursors was observed. (wikipedia.org)
  • Although those cells which expressed the markers were able to differentiate into muscle, adipogenic, osteogenic, nephrogenic, neural and endothelial cells, this did not necessarily occur from a homogenous population of undifferentiated cells. (wikipedia.org)
  • Neural precursor cells from adults have exceptional proliferative and differentiative capability in vitro yet respond minimally to in vivo brain injury due to constraining mechanisms that are poorly defined. (rupress.org)
  • We assessed whether cell cycle inhibitors that restrict stem cell populations in other tissues may participate in limiting neural stem cell reactivity in vivo. (rupress.org)
  • The cyclin-dependent kinase inhibitor, p21 cip1/waf1 (p21), maintains hematopoietic stem cell quiescence, and we evaluated its role in the regenerative response of neural tissue after ischemic injury using the mice deficient in p21. (rupress.org)
  • Here we report a method that enables the efficient generation and expansion of glial precursors from tissue-restricted neural stem cells (NSC). (scientificarchives.com)
  • These results demonstrate that tissue-restricted human neural stem cells can serve as an efficient source for myelinating oligodendrocytes with therapeutic potential. (scientificarchives.com)
  • This unmet medical need is a primary driver for the development of therapies based on transplantation of neural stem cells that have the potential to replace cells of the central nervous system (CNS) lost to disease or injury [1]. (scientificarchives.com)
  • The potential therapeutic value of oligodendrocytes has been demonstrated in animal studies by transplantation of oligodendrocyte precursor cells purified directly from neural tissue. (scientificarchives.com)
  • Expanded neural progenitors derived from regions of human fetal forebrain have been shown to have significant capacity to remyelinate rodent spinal cord upon grafting into areas of focal demyelination [9], and can myelinate host axons in a mouse model Pelizaeus-Merzbacher disease, which is characterized by severe hypomyelination [10]. (scientificarchives.com)
  • Similarly, recovery of locomotor function after grafting of neural stem cells (NSC) in animal models of spinal cord injury has been demonstrated to be due at least in part to myelinating oligodendrocytes [11]. (scientificarchives.com)
  • We demonstrate that neural stem/progenitor cell (NSPC) self-renewal and spatiotemporal generation of neurons and other cell types are severely impacted by the loss of Ythdf2 in embryonic neocortex. (biomedcentral.com)
  • and 3) the responses to directed neural differentiation was made between three NIH registered hES cell lines I3 (TE03), I6 (TE06) and BG01V. (biomedcentral.com)
  • All cell lines differentiated into three embryonic germ lineages in embryoid bodies and into neural cell lineages when cultured in neural differentiation medium. (biomedcentral.com)
  • Under the same neural differentiation-promoting conditions, the ability of each cell line to differentiate into neural progenitors varied. (biomedcentral.com)
  • In the present study, a side-by-side comparison of the ability to maintain an undifferentiated state and to self-renew under standard conditions, the ability to spontaneously differentiate into cell types of three germ layers in embryonic bodies, and directed differentiation under neural differentiation-promoting conditions was made between three NIH registered hESC lines I3, I6 and BG01V. (biomedcentral.com)
  • Amniotic stem cells are the mixture of stem cells that can be obtained from the amniotic fluid as well as the amniotic membrane. (wikipedia.org)
  • The stem cells are usually extracted from the amniotic sac by amniocentesis which occurs without harming the embryos. (wikipedia.org)
  • The use of amniotic fluid stem cells is therefore generally considered to lack the ethical problems associated with the use of cells from embryos. (wikipedia.org)
  • In 2009, the first US amniotic stem cell bank was opened in Medford, MA, by Biocell Center, an international company specializing in the cryopreservation and private banking of amniotic fluid stem cells. (wikipedia.org)
  • This was later confirmed as mesenchymal stem cells were obtained. (wikipedia.org)
  • In addition, evidence indicated that embryonic stem cells are part of the fluid, although in very small quantities. (wikipedia.org)
  • The majority of stem cells present in the amniotic fluid share many characteristics, which suggests they may have a common origin. (wikipedia.org)
  • This characteristic differentiates them from embryonic stem cells but indicates similarities with adult stem cells. (wikipedia.org)
  • However, foetal stem cells attained from the amniotic fluid are more stable and more plastic than their adult counterparts making it easier for them to be reprogrammed to a pluripotent state. (wikipedia.org)
  • A variety of techniques has been developed for the isolation and culturing of amniotic stem cells. (wikipedia.org)
  • There is some debate whether c-Kit is a suitable marker to distinguish amniotic stem cells from other cell types because cells lacking c-Kit also display differentiation potential. (wikipedia.org)
  • Mesenchymal stem cells (MSCs) are highly abundant in the amniotic fluid and several techniques have been described for their isolation. (wikipedia.org)
  • As opposed to mesenchymal stem cells, embryonic-like stem cells are not abundant in the amniotic fluid, making up less than 1% of amniocentesis samples. (wikipedia.org)
  • Embryonic-like stem cells were originally identified using markers common to embryonic stem cells such as nuclear Oct4, CD34, vimentin, alkaline phosphatase, stem cell factor and c-Kit. (wikipedia.org)
  • The pluripotency of these embryonic-like stem cells remains to be fully established. (wikipedia.org)
  • For example, RAD18 plays a vital role in regulating the DNA stability of embryonic stem cells and cellular homeostasis in highly prolific cells [ 6 ]. (nature.com)
  • Human trials using stem cells for remyelination have begun. (scientificarchives.com)
  • There have been several trials of mesenchymal stem cells injected intravenously, intrathecally or both in relapsing remitting and in progressive MS. Results suggest that mesenchymal stem cells can slow the progression of disease and might improve neurological function. (scientificarchives.com)
  • Previous molecular characterizations of mammary stem cells (MaSC) have utilized fluorescence-activated cell sorting or in vitro cultivation of cells from enzymatically dissociated tissue to enrich for MaSC. (frontiersin.org)
  • Insights into the biology of stem cells will be gained by confirmation and characterization of candidate MaSC markers identified in this study. (frontiersin.org)
  • This dictates cycles of mammary growth, differentiation, lactation, and regression, during which mammary stem cells (MaSC) provide for the lineages of luminal and basal (myoepithelial) epithelial cells in the ducts and alveoli. (frontiersin.org)
  • Retention of labeled DNA strands may be attributed to the ability of stem cells to retain the parental DNA strand during asymmetric cell division ( Cairns, 1975 ) or to quiescence of the stem cell population such that the DNA label is not diluted by frequent cell divisions ( Klein and Simons, 2011 ). (frontiersin.org)
  • In order to understand the development of adiposity, it is crucial to identify the factors and mechanisms that regulate the recruitment of mesenchymal stem cells (MSCs) of the vascular stromal fraction of the adipose tissue and its transformation into lipid-filled adipocytes. (biomedcentral.com)
  • Total and conditional knock out of PPARγ in hair follicle bulge stem cells showed similar result where scarring alopecia was observed in mice with the loss of pilosebaceous units and inflammatory cells infiltration 18 , 19 . (nature.com)
  • Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
  • Telomerase expression is also a hallmark of tissue stem cells, but its expression in these cells is still insufficient to completely prevent telomere shortening over time [ 11 ]. (biomedcentral.com)
  • Substantial data have provided convincing evidence establishing that human cancers emerge from cancer stem cells (CSCs), which display self-renewal and are resistant to anticancer drugs, radiation, and apoptosis, and express enhanced epithelial to mesenchymal progression. (oaepublish.com)
  • Can precancerous stem cells be risk markers for malignant transformation in the oral mucosa? (biomedcentral.com)
  • We speculate that precancerous stem cells (pCSCs) arise during the evolution of carcinomas based on long-term experimental findings, published literature, and the cancer stem cell (CSC) theory, wherein pCSCs exist in precancerous lesions and have characteristics of both CSCs and normal stem cells. (biomedcentral.com)
  • Therefore, investigations on testing and maintaining the integrity of genomic DNA within precancerous stem cells (pCSCs), which are within the framework of the cancer stem cell (CSC) hypothesis, are necessary. (biomedcentral.com)
  • Persistent stem cells in the oral epithelium of adults are the only cells that can accumulate sufficient mutations for OSCC to develop [ 18 , 19 ]. (biomedcentral.com)
  • In addition, normal adult stem cells (ASCs) self-renew, proliferate infinitely, have a multidirectional differentiation potential, similar to that of tumor cells, and can transform more easily into tumor stem cells than non-stem cells [ 20 , 21 ]. (biomedcentral.com)
  • The recurrence and drug resistance of breast cancer are intractable due to the presence of breast cancer stem cells (BCSCs), which are adequate to initiate tumor formation and refractory to conventional remedies. (spandidos-publications.com)
  • Of note, other studies have also indicated the involvement of RUNX2 in breast cancer stem cells (BCSCs) and breast cancer progression ( 22 , 23 ). (spandidos-publications.com)
  • A unique and essential property of embryonic stem cells is the ability to self-renew and differentiate into multiple cell lineages. (biomedcentral.com)
  • Human embryonic stem cells (hESCs) possess the ability to self-renew in an undifferentiated state in culture while retaining the ability to differentiate into all of the cell types in the human body. (biomedcentral.com)
  • To date, RUNX2 has been involved in diverse physiological processes, including osteogenic differentiation of mesenchymal stem/stromal cells, chondrocyte hypertrophy, immunomodulation, vascular invasion and endothelial cell migration via modulating a variety of signaling cascades (e.g. (spandidos-publications.com)
  • We have previously shown that fish primary preadipocytes differentiate into mature adipocytes in vitro and that these cells represent a very helpful model system to study adipose tissue development in fish [ 5 , 6 ]. (biomedcentral.com)
  • HCC heterogeneity is believed to be the outcome of combinatorial effects, including the acquisition of genetic and epigenetic alterations in tumor cells, the cross-talk of cells within the tumor microenvironment, as well as the existence of a cancer stem cell (CSC) subpopulation that has the ability to self-renew and differentiate. (molcells.org)
  • It is also essential to understand how the inherited variation in the sex, stage, quality and genetic background of embryos, as well as environmental influences such as derivation methods and passage procedures can affect the ability of hES cell lines to self-renew and differentiate. (biomedcentral.com)
  • The cells also have potential medical applications, especially in organ regeneration. (wikipedia.org)
  • These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)
  • Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)
  • Immunofluorescent double staining was used to detect YAP cellular localization with CD31 (an endothelial cell marker) antibody. (molvis.org)
  • In addition, NK cells stimulated with lunasin plus cytokines displayed higher tumoricidal activity than those stimulated with cytokines alone using in vitro and in vivo tumor models. (weeksmd.com)
  • Cells grown in vitro from neoplastic tissue. (lookformedical.com)
  • SMARCA2-downregulated pancreatic cancers cells had elevated chemosensitivity to gemcitabine in vitro and in vivo [85]. (morainetownshipdems.org)
  • The latter encompass human skin regenerated on immune deficient mice as well as organotypic constructs with epithelial and stromal cells embedded within architecturally faithful mesenchyma in vitro. (stanford.edu)
  • whereas LREC in suprabasal epithelial layers are enriched for more committed progenitor cells, expressing some genes that are associated with stem cell attributes along with those indicative of cell differentiation. (frontiersin.org)
  • These findings indicate miR-34a along with its putative target genes could play a role in RCC tumorigenesis and progression. (hindawi.com)
  • Eukaryotic expression vectors containing genes encoding plant proteins for killing of cancer cells. (weeksmd.com)
  • consequently, genes encoding some of these proteins are being used to design constructs for the inhibition of multiplying cancer cells. (weeksmd.com)
  • RNA was prepared separately from peripheral blood mononuclear cells (PBMC) and granulocytes to UNC1215 identify differentially expressed genes using whole genome microarrays. (tech-strategy.org)
  • Network analysis of the differentially expressed genes demonstrated that this steroid hormone receptor superfamily member hepatocyte nuclear factor 4 alpha (HNF4) is usually a hub in several of the gene networks that distinguished children with arthritis from controls. (tech-strategy.org)
  • The majority of mantle-cell lymphomas are associated with a t(11;14) translocation resulting in overexpression of the CYCLIN D1 gene (GENES, BCL-1). (lookformedical.com)
  • Nuclear PKM2 interacts with HIF-1α and subsequently upregulates the expression of HIF-1α target genes encoding glycolytic enzymes, GLUT1, HK2, PKM2, LDHA and PDK1, which are required for the C. tropicalis -induced aerobic glycolysis of MDSCs. (biomedcentral.com)
  • the development of T cells, we generated a mouse strain having a disruption in both CD3 and CD3 genes (CD3? (tech-strategy.org)
  • Proliferating cell nuclear antigen (PCNA) expression in CNV mice without or with YAP siRNA intravitreal injection and the colocalization of PCNA and CD31 were measured with western blotting and immunofluorescent double staining, respectively. (molvis.org)
  • Additionally, after laser exposure, YAP siRNA inhibited the increased expression of PCNA, which was colocalized with endothelial cells. (molvis.org)
  • they frequently sent one daughter cell to a non-surface (NS)position, the subventricular zone (SVZ), within a single cell-cycle length while keeping the other mitotic daughter for division at the surface. (biologists.com)
  • i.p) or vehicle administered 24 hours after permanent middle cerebral artery occlusion (pMCAO) on behavior, angiogenesis, ultra-structural integrity of brain capillary endothelial cells, and expression of EPO and VEGF were assessed. (researchgate.net)
  • These results suggested that catalpol might contribute to infarcted-brain angiogenesis and ameliorate the edema of brain capillary endothelial cells (BCECs) by upregulating VEGF and EPO coordinately. (researchgate.net)
  • This chapter provides an overview of the key role of hEPC in promoting angiogenesis and their potential use for cell therapy. (intechopen.com)
  • Furthermore, generation of glial precursors from sources such as embryonic stem (ES) cells and induced pluripotent stem (iPS) cells poses potential safety risks due to the tumorigenic potential of undifferentiated cells. (scientificarchives.com)
  • although it is derived from cells of the renal tubular epithelium, it has several histological subtypes which differ in their clinical outcome and biological features. (hindawi.com)
  • Different phenotypes and subtypes of endothelial progenitor cells (EPCs), such as early and late EPCs, have been described according to their functionality. (intechopen.com)
  • Traditionally, reporting DNA content has been accomplished with a high degree of accuracy in large cell populations using flow cytometry, a technique that allows the proportion of cells in each phase of the cell cycle to be calculated in a high-throughput fashion. (biomedcentral.com)
  • However, the measurement of DNA content by flow cytometry provides little resolution to the biology of individual cells. (biomedcentral.com)
  • GEP analyses were performed on highly purified, flow-cytometry sorted tumor-cells from eight subgroups of BCLs. (lu.se)
  • In this study, highly purified, flow-cytometry sorted, classified in relation to normal B-cell differentiation [1]. (lu.se)
  • Using immunocytochemistry, flow cytometry, qRT-PCR and MPSS, we found that all three cell lines actively proliferated and expressed similar "stemness" markers including transcription factors POU5F1/Oct3/4 and NANOG, glycolipids SSEA4 and TRA-1-81, and alkaline phosphatase activity. (biomedcentral.com)
  • The 'adipose tissue expandability model' identifies the limited capacity and dysfunctionality of WAT, preventing its expansion and accommodation of surplus of energy, as key determinants for the onset and progression of obesity-associated metabolopathologies as a result of ectopic deposition of toxic lipid species in metabolic organs (i.e. muscle or liver [also known as lipotoxic insult]) [ 7 ]. (springer.com)
  • These approaches result in the loss of all histological information pertaining to the in vivo locale of MaSC and progenitor cells. (frontiersin.org)
  • The availability of a sophisticated genetic tool box makes Drosophila an excellent genetic model well suited to studying the biology of Lunasin and its effect on tumor progression in an in vivo model organism. (weeksmd.com)
  • HHV-8 infection induces also HIV reactivation in chronically infected cell lines and in peripheral blood mononuclear cells (PBMCs) from patients with asymptomatic HIV, suggesting the possibility that similar interactions might take place also in vivo. (ashpublications.org)
  • In this study we asked whether global epigenetic mechanisms regulate timing of progenitor cell differentiation into myelin-forming oligodendrocytes in vivo. (rupress.org)
  • Glial cells play a critical role in the development and function of the mammalian central nervous system (CNS). (scientificarchives.com)
  • Transplantation of glial cells or glial progenitors into the diseased or injured CNS can provide therapeutic benefits. (scientificarchives.com)
  • In response to endogenous and exogenous insults, malignant cells mostly have an intensive DNA repair capacity that allows them to proliferate and survive. (nature.com)
  • Numerous vectors have been engineered for the sole purpose of killing cancer cells, and some have successfully suppressed malignant tumours. (weeksmd.com)
  • As with all tumors, whether benign or malignant, the paradigm of comprehension depends on identifying the cell or cell layer of origin. (medscape.com)
  • A group of heterogeneous lymphoid tumors generally expressing one or more B-cell antigens or representing malignant transformations of B-lymphocytes. (lookformedical.com)
  • Any of a group of malignant tumors of lymphoid tissue that differ from HODGKIN DISEASE, being more heterogeneous with respect to malignant cell lineage, clinical course, prognosis, and therapy. (lookformedical.com)
  • Malignant lymphoma composed of large B lymphoid cells whose nuclear size can exceed normal macrophage nuclei, or more than twice the size of a normal lymphocyte. (lookformedical.com)
  • Malignant lymphoma in which the lymphomatous cells are clustered into identifiable nodules within the LYMPH NODES. (lookformedical.com)
  • The cancer stem cell (CSC) paradigm emerged from investigating a subpopulation of less-differentiated CD34+/CD38- cells possessing stem cell-like renewal ability and robust malignant-initiating capacity in acute myeloid leukemia (AML) [ 1 ] . (oaepublish.com)
  • ALL likely begins with the malignant transformation of either B or T cell progenitor cells. (amgenoncology.com)
  • In addition, all of these markers can occur on their own or in some combination in other types of cells. (wikipedia.org)
  • Our results support the use of DNA label retention to identify MaSC and also provide a molecular profile and novel candidate markers for these cells. (frontiersin.org)
  • In contrast, exposure of MCF7 cells to curcumin resulted in increased global levels of acetylated H3K18 and H4K16, and was less effective in inducing DNA damage markers. (biomedcentral.com)
  • During the first 10 postnatal days, administration of valproic acid (VPA), the specific inhibitor for histone deacetylase activity, resulted in significant hypomyelination with delayed expression of late differentiation markers and retained expression of progenitor markers. (rupress.org)
  • Description: The G8.8 monoclonal antibody reacts with the 40 kDa mouse EpCAM (epithelial cellular adhesion molecule), also known as EGP40 (epithelial glycoprotein 40), 17-1A antigen, TACSTD1 (tumor-associated calcium signal transducer 1), and CD326. (thermofisher.com)
  • The immunogen used to generate the G8.8 antibody was the TE-71 thymic epithelial cell line. (thermofisher.com)
  • Applications Tested: This G8.8 antibody has been tested by flow cytometric analysis of the TE-71 cell line. (thermofisher.com)
  • A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)
  • Undifferentiated I3 cells grew significantly slower but their differentiation potential was greater than I6 and BG01V. (biomedcentral.com)
  • in adult acinar cells harboring oncogenic mutation accelerated acinar to ductal reprogramming resulting in mucinous PDAC precursor lesions in mice. (morainetownshipdems.org)
  • In contrast to mice deficient in CH5424802 biological activity either CD3 or CD3 chains, early thymic development mediated by pre-TCR is completely clogged, and TCR-+ or TCR-+ T cells were absent in the CD3? (tech-strategy.org)
  • Since T cells normally take into account only an extremely small percentage of thymocytes and peripheral T cells, we evaluated T cell advancement in the tiny intestine, where T cells represent a significant population from the iIEL in wild-type mice. (tech-strategy.org)
  • mice, T cells had been once again nondetectable in the intestine (Fig. (tech-strategy.org)
  • Our previous study showed that it plays an essential role in the progression of multiple tumors. (nature.com)
  • TAMs are the major inflammatory cells in the TME, and are closely related to poor outcomes in several types of tumors [ 11 ]. (nature.com)
  • Skin malignancies, Merkel cell carcinoma and rare appendageal tumors. (medscape.com)
  • The only common feature among these tumors is the absence of giant REED-STERNBERG CELLS, a characteristic of Hodgkin's disease. (lookformedical.com)
  • B-cell lymphoid tumors that occur in association with AIDS. (lookformedical.com)
  • The biological importance of activation targets of Nanog, Oct4, SOX-2, and c-Myc in CSCs, which are more frequently overexpressed in poorly differentiated tumors than in well-differentiated tumors, has been shown by correlating signature characteristics of these cells and poor survival [ 3 ] . (oaepublish.com)
  • According to the hypothesis, only a small group of stem cell-like tumor cells can form new tumors. (biomedcentral.com)
  • B-cell lymphomas (BCLs) constitute a diverse set of tially identify new functional, diagnostic, and therapeutic tumors, both morphologically and clinically, that are mainly targets. (lu.se)
  • Haematopoietic progenitor cells were first reported to be present in the amniotic fluid in 1993, specifically up to the 12th week of pregnancy. (wikipedia.org)
  • In 1996, a study indicated that non-haematopoietic progenitor cells were also present in the amniotic fluid. (wikipedia.org)
  • Notably, mpeg1.1 + macrophages have much longer telomeres in the gut than in their haematopoietic tissue of origin, suggesting that there is modulation of telomerase in these cells, in the gut. (biomedcentral.com)
  • One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). (lookformedical.com)
  • Then, we will discuss several principles that govern macrophage phagocytosis of apoptotic cells (efferocytosis), including the existence of redundant recognition mechanisms ("find-me" and "eat-me") that lead to a tight coupling between apoptosis and phagocytosis. (frontiersin.org)
  • Our previous study has revealed that Candida tropicalis ( C. tropicalis ) promotes colorectal tumorigenesis by enhancing immunosuppressive function of myeloid-derived suppressor cells (MDSCs) and increasing accumulation of MDSCs, but the underlying mechanisms remain unestablished. (biomedcentral.com)
  • Studying such mechanisms not only provides mechanistic insights into these cells that are unresponsive to drugs, but may lead to the development of targeted and effective therapeutics to eradicate CSCs. (oaepublish.com)
  • Epigenetic control of gene expression lasts through multiple cell divisions without alterations in primary DNA sequence and can occur via mechanisms that include histone modification and DNA methylation. (stanford.edu)
  • Peripheral blood mononuclear cells of healthy donors and post-transplant lymphoma patients were stimulated with or without lunasin in the presence of IL-12 or IL-2. (weeksmd.com)
  • In adult mouse hippocampus, Tbr2 protein and Tbr2-GFP (green fluorescent protein) transgene expression were specifically localized to intermediate-stage progenitor cells (IPCs), a type of transit amplifying cells. (jneurosci.org)
  • Remarkably, the same TF cascade was found to be linked to stages of neuronal lineage progression in adult SGZ. (jneurosci.org)
  • suggesting that progenitor cells in the embryonic and adult brain may share similar molecular phenotypes. (jneurosci.org)
  • The present experiments were designed to determine whether aspects of this TF cascade are conserved in the adult DG and to examine the identity of Tbr2+ cells in the SGZ. (jneurosci.org)
  • Experiments were also conducted to assess the responses of Tbr2+ cells to neurogenic stimuli, with the goal of understanding how Tbr2+ IPCs contribute to neuron production in the adult DG. (jneurosci.org)
  • One group demonstrated that cells dissected from fetal human forebrain or adult white matter and selected using immunosorting methods could be transplanted into myelin-deficient animals and provide significant myelination of host neurons [5-8]. (scientificarchives.com)
  • In later embryonic stages and in adult organisms, E-cad also provides adhesive strength to form polarized epithelial cell layers [ 2 ]. (biomedcentral.com)
  • On the other hand, clear cell acanthoma is a rare, benign epithelial cutaneous tumor. (medscape.com)
  • We isolated four categories of cells from mammary epithelium of female calves: bromodeoxyuridine label retaining epithelial cells (LREC) from basal (LRECb) and embedded layers (LRECe), and epithelial control cells from basal and embedded layers. (frontiersin.org)
  • Of note, differentiated goblet cells retained longer telomeres than columnar lined BE epithelium. (oncotarget.com)
  • In conclusion, our studies suggest that shortened telomeres are functionally important for tumor development in a mouse model of BE and are associated with proliferating columnar epithelium in human BE. (oncotarget.com)
  • Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule. (thermofisher.com)
  • ar4280-S8.PDF (1.7M) GUID:?3B4F6A60-6238-4C1B-845B-025DC69E0C96 Abstract Introduction The attainment of remission has become an important end point for clinical trials in juvenile idiopathic arthritis (JIA), although we do not yet have a full understanding of what remission is at the cell and molecular level. (tech-strategy.org)
  • In fact, clear cell acanthoma possesses a similar staining pattern to inflammatory dermatoses such as psoriasis vulgaris, lichen planus, and discoid lupus erythematosus and might be a localized form of inflammatory eruption rather than a neoplasm. (medscape.com)
  • Renal cell carcinoma (RCC) incidence has increased over the past two decades. (hindawi.com)
  • Renal cell carcinoma (RCC) accounts for approximately 3% of human malignancies, and its incidence appears to be increasing globally [ 1 ]. (hindawi.com)
  • For instance, RUNX2 is involved in the progression of various tumor types, such as osteosarcoma, renal cell carcinoma, gastric cancer and breast cancer ( 15 - 20 ). (spandidos-publications.com)
  • If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely. (lookformedical.com)
  • CSCs represent a heterogeneous tumor cell population and lack specific cellular targets, which makes it a great challenge to target and eradicate them. (oaepublish.com)
  • However, most GEP studies have typically been performed on whole tissue samples, containing varying degrees of tumor cell content, which results in uncertainties in data analysis. (lu.se)
  • We found that the sole presence of N-cad induces constitutively active fibroblast growth factor (Fgf) signaling and a precocious involution resulting in massive apoptosis of alveolar cells. (biomedcentral.com)
  • Both establish calcium-dependent homophilic cell-cell adhesion with their extracellular domains and are connected with catenins at their intracellular domains [ 4 ]. (biomedcentral.com)
  • CD326 mediates calcium-independent, homophilic, cell-cell adhesion and may function as a growth factor receptor. (thermofisher.com)
  • The combination of lunasin and cytokines (IL-12 plus IL-2) was capable of restoring IFNγ production by NK cells from post-transplant lymphoma patients. (weeksmd.com)
  • Lymphoma b cell. (lookformedical.com)
  • B-cell antigens are expressed on the immature cells that make up the tumor in virtually all cases of Burkitt lymphoma. (lookformedical.com)
  • A form of non-Hodgkin lymphoma having a usually diffuse pattern with both small and medium lymphocytes and small cleaved cells. (lookformedical.com)
  • This enabled identification of TFs that can be uniquely associated to the tumor cells of chronic lymphocytic leukemia (CLL), diffuse large B-cell lymphoma (DLBCL), follicular lymphoma (FL), hairy cell leukemia (HCL), and mantle cell lymphoma (MCL). (lu.se)
  • Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. (lookformedical.com)
  • Skin malignancies, including epidermal squamous cell carcinoma (SCC), alone account for nearly as many cancers as all other tissues combined. (stanford.edu)
  • Human leukocyte antigen testing has been utilised to confirm that the MSCs stem from the fetus and not from the mother. (wikipedia.org)
  • Hazel T, Hefferan M, Schwartz K, Yu N, Johe K, Levy M. Generation of Human Oligodendrocyte Progenitors for Treatment of Demyelinating Diseases and Spinal Cord Injury. (scientificarchives.com)
  • METHODS: Exposing human cardiomyocyte AC16 cells to varying concentrations of Wnt10b and DOX, we observed key metrics of cell viability. (bvsalud.org)
  • In an anagen human hair follicle PPARγ expression is detected in the mesenchymal DP cells, epithelial cells of the outer root sheath (ORS), inner root sheath and matrix 16 . (nature.com)
  • In addition, pilot studies of human BE-, dysplasia- and EAC tissue samples confirmed that BE epithelial cells with or without dysplasia (LGD) had shorter telomeres compared to gastric cardia tissue. (oncotarget.com)
  • Previous results indicate that the HHV-8 viral transactivator ORF50 interacts synergistically with Tat protein in the transactivation of human immunodeficiency virus (HIV) long terminal repeat (LTR), leading to increased cell susceptibility to HIV infection. (ashpublications.org)
  • Telomerase, the enzyme capable of elongating telomeres, is usually restricted in human somatic cells, which contributes to progressive telomere shortening with cell-division and ageing. (biomedcentral.com)
  • Our experimental focus is on the mammalian setting using multiomics, informatics, mouse genetics, human genetics, single cell studies, and new human tissue platforms. (stanford.edu)
  • The antigen is being used as a target for immunotherapy treatment of human carcinomas. (thermofisher.com)
  • Additionally, RAD18 functions beyond DNA repair and may regulate many other key biological processes, such as chromatin strengthening, cell survival/death, stemness, and differentiation. (nature.com)
  • Among other roles, these cells provide the myelin sheath needed for the efficient propagation of impulses along nerve fibers, provide trophic support for neuronal cells, and remove toxins and excess neurotransmitters from the interstitial space. (scientificarchives.com)
  • The NS-dividing cells were mostly Hu + and their daughters were also Hu + , suggesting their commitment to the neuronal lineage and supply of early neurons at a position much closer to their destiny than from the ventricular surface. (biologists.com)
  • and cell differentiation ( SOX2 and TGFB3 ) as well as immunohistochemical assay for VEGFA, TP53, Bcl2, TGFB1, and Ki67 protein expression have been performed in 85 FFPE RCC tumor specimens. (hindawi.com)
  • Presented in conjunction with cells expressing a histone 2B-GFP fusion protein (H2B-GFP), we demonstrated how this method enabled chromosomal segregation errors to be tracked in cells as they progressed through cellular division that were later identified as either diploid or polyploid. (biomedcentral.com)
  • DeMarini, and Chapter 20, by Rice and cell death determine the size protein in several signal ing path- and Herceg). (who.int)
  • From development to aging and disease, the brain parenchyma is under the constant threat of debris accumulation, in the form of dead cells and protein aggregates. (frontiersin.org)
  • Tumor microenvironment (TME) remodeling is a critical process in primary tumor progression. (nature.com)
  • Primary or transformed monocytic and endothelial cells were infected with a cell-free HHV-8 inoculum and subsequently infected with lymphotropic or monocytotropic strains of HIV. (ashpublications.org)
  • Instead, we used laser microdissection to excise putative progenitor cells and control cells from their in situ locations in cryosections and characterized the molecular properties of these cells. (frontiersin.org)
  • Adipogenesis has been extensively studied in mammals [ 7 ] and several cell and animal models have been successfully used to describe the chronology of the molecular events governing this process. (biomedcentral.com)
  • Blockade of PKM2 nuclear translocation attenuates C. tropicalis -mediated colorectal tumorigenesis. (biomedcentral.com)
  • However, an accumulation of fibrotic tissue and cysts with increasing age and lactation cycles was observed. (biomedcentral.com)
  • Errors arising in stem and progenitor cells have a much greater influence on the tissue in which they are found than errors arising in postmitotic differentiated cells. (biomedcentral.com)
  • Notably, the Tbr2+ IPCs formed cellular clusters, the average size of which (Tbr2+ cells per cluster) likewise more than doubled in runners. (jneurosci.org)
  • Live-cell fluorescence microscopy (LCFM) is a powerful tool used to investigate cellular dynamics in real time. (biomedcentral.com)
  • The ability to measure DNA content in single cells by means of LCFM would allow cellular stage and ploidy to be coupled with a variety of imaging directed analyses. (biomedcentral.com)
  • This method allows the examination of single-cell dynamics to be correlated with cellular stage and ploidy in a high-throughput fashion. (biomedcentral.com)
  • Collectively, we anticipate that this method will allow high-resolution microscopic analysis of cellular processes involving cell cycle progression, such as checkpoint activation, DNA replication, and cellular division. (biomedcentral.com)
  • Its quantification serves to assess several cellular parameters including DNA ploidy and a cell's temporal location within the cell cycle [ 1 ]. (biomedcentral.com)
  • To overcome this limitation, approaches incorporating laser scanning cytometry and fluorescence microscopy have been successful in quantifying DNA content in single cells [ 2 , 3 ], providing the capacity to combine this information with additional cellular parameters that can be elucidated by imaging. (biomedcentral.com)
  • Therefore, traditional methods for examining DNA content by microscopy have relied on cellular fixation, which is incompatible with tracking cells over time. (biomedcentral.com)
  • To overcome the limitations brought upon by cellular fixation, membrane-permeant DNA fluorophores were designed to stain DNA stoichiometrically in live-cells (supravital staining), enabling both the quantification of DNA content and the ability to track the movement of chromosomes over time. (biomedcentral.com)
  • They usually involve the removal of amniotic fluid by amniocentesis and their distinction from other cells may be based on their morphology or other characteristics. (wikipedia.org)
  • OBJECTIVES: To describe the morphologic characteristics of skin lesions, extent of extracutaneous disease, and outcomes in patients with neonatal presentation of Langerhans cell histiocytosis (LCH), and to examine clinical predictors of disease prognosis. (thedoctorsdoctor.com)
  • Drug-like molecules that can reprogram selected histone PTMs in tumour cells are therefore of interest as potential cancer chemopreventive agents. (biomedcentral.com)
  • In this study we assessed the effects of the phytocompounds garcinol and curcumin on histone and p53 modification in cancer cells, focussing on the breast tumour cell line MCF7. (biomedcentral.com)
  • In summary, although garcinol and curcumin can both inhibit histone acetyltransferase activities, our results show that these compounds have differential effects on cancer cells in culture. (biomedcentral.com)
  • Garcinol treatment alters expression of chromatin modifying enzymes in MCF7 cells, resulting in reprogramming of key histone and p53 PTMs and growth arrest, underscoring its potential as a cancer chemopreventive agent. (biomedcentral.com)
  • Thus, drug-like molecules that target chromatin modifying enzymes to reprogram selected histone PTMs in tumour cells may have potential as cancer chemopreventive agents. (biomedcentral.com)
  • The function of histone modifying epigenetic regulators and noncoding RNA as central mediators of epithelial stem cell renewal and differentiation represent major emerging areas of study in the lab. (stanford.edu)
  • We are currently pursuing studies of the dominant signaling and gene regulatory networks that control this process, including the Ras/MAPK cascade, which is required for stem cell-mediated self-renewal and the p53 transcription factor family member, p63, which is required for epidermal differentiation. (stanford.edu)
  • Transcription factors (TFs) are critical for B-cell differentiation, affecting gene expression both by repres- sion and transcriptional activation. (lu.se)
  • However, the mechanism through which RAD18 influences triple-negative breast cancer (TNBC), especially the interaction between tumor cells and the tumor microenvironment, remains elusive. (nature.com)
  • One such peptide, Lunasin , has been shown to selectively act on newly transformed cells while having no cytotoxic effect on non-tumorigenic or established cancer cell lines. (weeksmd.com)
  • Gene therapy has attracted attention for its potential to specifically and efficiently target cancer cells with minimal toxicity to normal cells. (weeksmd.com)
  • BE- development and its progression to cancer is associated with gastroesophageal reflux disease. (oncotarget.com)
  • And more recently, we discovered that C. tropicalis promoted colitis-associated colon cancer (CAC) through enhancing the accumulation and immunosuppressive activity of myeloid-derived suppressor cells (MDSCs) [ 10 ]. (biomedcentral.com)
  • The present review mainly focused on the latest updates on RUNX2 in BCSCs and their roles in breast cancer progression and drug resistance, providing insight that may aid the development of RUNX2‑based diagnostics and treatments for breast cancer in clinical practice. (spandidos-publications.com)
  • In addition, there is accumulating evidence in recent years to show that the malignancy of HCC can be attributed partly to the presence of cancer stem cell (CSC). (molcells.org)
  • Distinct functions of macrophage-derived and cancer cell-derived cathepsin Z combine to promote tumor malignancy via interactions with the extracellular matrix. (uni-freiburg.de)
  • On the other hand, C. tropicalis promotes p-Syk binding to PKM2, which results in PKM2 Tyr105 phosphorylation and PKM2 nuclear translocation in MDSCs. (biomedcentral.com)