Progenitor cell differentiation. Medical search. Frequent questions

Transcription factor MEF2C influences neural stem/progenitor cell differentiation and maturation in vivo. (ca.gov)
Here, we show that conditional knockout of Mef2c in nestin-expressing neural stem/progenitor cells (NSCs) impaired neuronal differentiation in vivo, resulting in aberrant compaction and smaller somal size. (ca.gov)
Here we define a 4-color flow cytometry panel using CD133, LeX, CD140a, NG2 to define a neural stem cell (NSC) as well as 4 classes of multipotential progenitors and 3 classes of bipotential progenitors, several of which have not been described previously. (karger.com)
Growing evidence suggests that the clinical use of neural progenitor cells (NPCs) is hampered by heterogeneity, poor neuronal yield and low survival rate. (elsevierpure.com)
Numerous studies have shown that cell replacement therapy can replenish lost cells and rebuild neural circuitry in animal models of Parkinson's disease . (bvsalud.org)
This study aimed at elucidating the effect of major non-THC phytocannabinoids on the fate of adult neural stem progenitor cells (NSPCs), which are an essential component of brain function in health as well as in pathology. (nih.gov)
Neural Progenitor Cells Derived from XCL-1 DCXp-GFP are a neuronal lineage reporter cell line that can be used in neuronal differentiation and drug screening. (atcc.org)
To download a certificate of analysis for Neural Progenitor Cells Derived from XCL-1 DCXp-GFP ( ACS-5005 ), enter the lot number exactly as it appears on your product label or packing slip. (atcc.org)
The certificate of analysis for that lot of Neural Progenitor Cells Derived from XCL-1 DCXp-GFP ( ACS-5005 ) is not currently available online. (atcc.org)
In this study, we used iPSC-derived neural progenitor cells (NPCs) from individuals with ASD of known and unknown genetic cause as well as from neurotypical controls to examine the effects of exogenous IL-17a on NPC proliferation, migration and neuronal differentiation, and whether IL-17a and genetic risk factors for ASD interact exacerbating alterations in NPC function. (figshare.com)
In particular, the CHD8 protein and the genes it regulates likely help control the development of neural progenitor cells, which give rise to nerve cells (neurons), and the growth and division (proliferation) and maturation (differentiation) of neurons. (medlineplus.gov)
To circumvent these obstacles, we have developed two methods for the investigation of human neural cells in culture. (lu.se)
Firstly, we present a robust 2-week protocol for the differentiation of human pluripotent stem cells (PSCs) into forebrain neural progenitor cells. (lu.se)
The focus of my research is to understand cell fate specification in the developing brain and in human neural progenitor cells using cell-based models of neuronal differentiation. (lu.se)

Cells in the blood such as immune cells, red blood cells and other vital cell types are constantly renewed from stem cells, the so-called hematopoietic stem and progenitor cells (HSPCs). (phys.org)
Endothelial cells (ECs) line blood vessels and can serve as specialized vascular niches for hematopoietic stem and progenitor cells (HSPCs), a special environment where HSPCs reside and self-renew. (phys.org)
Genetic abnormalities associated with MDS block differentiation of hematopoietic stem and progenitor cells. (medscape.com)

The goal of this protocol is to test the ability of progenitor cells derived from human perivascular adipose tissue to differentiate into multiple cell lineages. (jove.com)
Differentiation was compared to mesenchymal stem cells derived from human bone marrow, which is known to differentiate into adipocyte, osteocyte, and chondrocyte lineages. (jove.com)
Such cells from different sources can proliferate and differentiate into different lineages (e.g. osteogenic, chondrogenic and adipogenic) after suitable stimulation. (scirp.org)
The progenitor cells of the developing liver can differentiate toward both hepatocyte and biliary cell fates. (elifesciences.org)
Unlike myoblasts, reserve cells do not terminally differentiate but return to quiescence. (heika-research.de)
Stem cells rely on extracellular signals produced by the niche, which dictate their ability to self-renew, expand and differentiate. (karger.com)
Liver stem/progenitor cells have the potential to differentiate to form hepatocytes or cholangiocytes. (elsevierpure.com)
Pancreatic progenitor cells are multipotent stem cells originating from the developing fore-gut endoderm which have the ability to differentiate into the lineage specific progenitors responsible for the developing pancreas. (wikipedia.org)
These organoids harbor a subpopulation of ALDH hi cells that display proliferative capacity and can differentiate to an endocrine fate. (prinsesmaximacentrum.nl)
Human pluripotent stem cells, with their ability to proliferate indefinitely and to differentiate into virtually all cell types of the human body, provide a novel resource to study human development and to implement relevant disease models. (mdpi.com)
In this context, if it is possible to immortalize normal human SAE BC that retain the capacity to differentiate to ciliated, secretory, and other differentiated cell types in vitro on air-liquid interface (ALI) culture, it would be very useful to the investigation of SAE biology in health and disease, and the assessment of pharmacologic agents targeted to modify dysregulated BC biology relevant to the pathogenesis of human lung disease. (biomedcentral.com)
Human embryonic stem cells (hESCs) derived from an early embryo can self-renew indefinitely and differentiate into a variety of cell types. (ashpublications.org)
Like stem cells, progenitor cells have a capacity to differentiate into a specific type of cell. (phys.org)
In contrast to stem cells, however, they are already far more specific: they are pushed to differentiate into their "target" cell. (phys.org)
Mesenchymal stem cells (MSCs) are multipotent stem/progenitor cells which can differentiate to several mesodermal derivatives and possess an ability to secrete factors involved in neovascularization and immunomodulation [ 1 , 2 ]. (hindawi.com)
On the same day, the cells in the apical chamber were exposed to air (airlift) to create the ALI and allowed to differentiate for 28 days. (cdc.gov)
We show that human and chimpanzee cells differentiate in a similar man¬ner and that the difference in interspecies protein abundance is higher than transcript-level differences, suggesting that post-transcriptional mechanisms play a role in the difference between human and chim¬panzee brain development. (lu.se)
Watch the lecture where Malin Parmar tells about the long history of transplantation trials in Lund and how they have been able to differentiate stem cells into specific dopaminergic cells in vitro. (lu.se)

Perturbation of addition of second heart field (SHF) cardiac progenitor cells to the poles of the heart tube results in congenital heart defects (CHD). (hal.science)
Cardiac progenitor cells (CPCs), migrating from heart tissue, in culture aggregate to form cardiospheres (CSs) in which replication and cardiogenic differentiation occur. (unimib.it)
Despite extensive stem cell therapy studies, clinical trials using cardiac progenitor cells have shown moderate results. (ox.ac.uk)

However, details regarding the regulation of MSC/MPC proliferation and differentiation status are still unclear and it is likely that regulation involves both biological and mechanical influences in the different environments. (scirp.org)
Altogether our FACS (fluorescence-activated cell sorter) analyses reveal that the neonatal subventricular zone is far more heterogeneous than previously suspected and our studies provide new insights into the signals and mechanisms that regulate their self-renewal and proliferation. (karger.com)
Developmentally regulated EZH2 levels are one of the factors that can determine the higher order chromatin structure and expression pattern of the INK4b-ARF-INK4a locus, coupling human progenitor cell differentiation to proliferation control. (biomedcentral.com)
Development and homeostasis require the coordinate regulation of cell proliferation and differentiation. (biomedcentral.com)
Mechanistically, EBF2 uniformly activates Pparγ-expression in pancreatic cancer cells and limits cell proliferation, whereas oncogenic drivers are expressed differentially in the progenitor cell subsets. (amegroups.org)
The complex PDAC pathobiology is established predominantly through KRAS mutations and the associated cellular signaling that contributes to cell proliferation and dedifferentiation ( 5 , 6 ). (amegroups.org)
Interleukin 13: novel role in direct regulation of proliferation and differentiation of primitive hematopoietic progenitor cells. (ox.ac.uk)
IL-13 enhanced stem cell factor (SCF)-induced proliferation of Lin-Sca-1+ bone marrow progenitor cells more potently than IL-4. (ox.ac.uk)
In contrast, IL-13 had no synergistic activity on SCF-induced proliferation of the more mature Lin-Sca-1- progenitor cells. (ox.ac.uk)
Furthermore, IL-13 but not IL-4 synergistically enhanced colony formation of Lin-Sca-1+ progenitors in response to granulocyte/macrophage colony-stimulating factor (GM-CSF) (threefold), whereas both IL-4 and IL-13 enhanced G-CSF-induced colony formation (threefold), and neither of the two significantly affected CSF-1 and IL-3-induced proliferation. (ox.ac.uk)
Exogenous IL-17a did not induce abnormal proliferation and migration of ASD and control NPCs but, on the other hand, it significantly increased the expression of synaptic (Synaptophysin-1, Synapsin-1) and neuronal polarity (MAP2) proteins in these cells. (figshare.com)
For several decades, neovascularization is believed to be accomplished by proliferation of mature endothelial cells residing in the local vessels through the process of angiogenesis [ 6 ]. (hindawi.com)
The mitochondrial electron transport chain is dispensable for proliferation and differentiation of epidermal progenitor cells. (nature.com)
Proliferation-linked shifts in eligibility of T-cell progenitors, population dynamics, DN1 cells to undergo specification thus control kinetics of T-cell generation. (lu.se)

Cultured oligodendrocyte progenitor cells (OPCs) were incubated with HD ± BMSC-derived conditional medium (BMSC-CM). OPC differentiation was studied by immunostaining and morphometric analysis. (biomedcentral.com)

Our data support a crucial role for MEF2C in programming early neuronal differentiation and proper distribution within the layers of the neocortex. (ca.gov)
The hADC gene delivery triggered higher expression of N-CAM, cell adhesion molecule and MAP-2, neuronal marker. (elsevierpure.com)
However, the hADC gene knockdown showed downregulation of N-CAM and MAP-2 expression suggesting that hADC gene delivery favors cell fate commitment of mNPCs towards neuronal lineage. (elsevierpure.com)
The neurotrophic signal, BDNF aided in the neuronal commitment, differentiation, and maturation of hADC-mNPCs through PI3K and ERK1/2 activation. (elsevierpure.com)
Our findings suggest that hADC gene delivery favors cell fate commitment of mNPCs towards neuronal lineage, bring new advances in the field of neurogenesis and cell therapy. (elsevierpure.com)
In conclusion, our results suggest that exogenous IL-17a positively regulates the neuronal differentiation of human NPCs, which may disturb normal neuronal and synaptic development and contribute to MIA-related changes in brain function and behavior. (figshare.com)
Shane Grealish: Cell Replacement Therapy for Parkinson's Disease: The Importance of Neuronal Subtype, Cell Source and Connectivity for Functional Recovery. (lu.se)
The aim of this thesis was to understand how particular factors such as neuronal content, placement and cell source, affect functional outcome after transplantation into the rodent brain. (lu.se)
Through understanding functional recovery in terms of neuronal subtype and connectivity, the work presented in this thesis aims to bring the prospect of CRT closer to the clinic, I also describe the generation of a very promising alternative cell source that could rival fetal tissue. (lu.se)

Here, we profile the transcriptome and chromatin accessibility of anterior and posterior SHF sub-populations at genome-wide levels and demonstrate that Hoxb1 negatively regulates differentiation in the posterior SHF. (hal.science)
We show that NAD + regulates CD4 + T-cell differentiation through tryptophan hydroxylase-1 ( Tph1 ), independently of well-established transcription factors. (nature.com)
These genes regulates the expression of Mnx1 in the ventral bud, leading to the developmental specification of the pancreatic progenitor cells expressing Pdx1. (wikipedia.org)
Extracellular matrix (ECM) also regulates the differentiation by MPCs. (tmu.edu.tw)

Hart, D.A. (2014) Why Mesenchymal Stem Cell/Progenitor Cell Heterogeneity in Specific Environments? (scirp.org)
Wu, Y. and Zhao, R.C. (2012) The Role of Chemokines in Mesenchymal Stem Cell Homing to the Myocardium. (scirp.org)
Stem Cell Reviews, 8, 243-250. (scirp.org)
Li, L. and Jiang, J. (2011) Regulatory Factors of Mesenchymal Stem Cell Migration into Injured Tissues and Their Signal Transduction Mechanisms. (scirp.org)
Fehrer, C. and Lepperdinger, G. (2005) Mesenchymal Stem Cell Aging. (scirp.org)
Stem Cell Research , 17 (2), 256-265. (elsevierpure.com)
This transcription factor has been shown to give rise to the multipotent stem cell lineages contributing to the endocrine, exocrine and ductal cells of the pancreas. (wikipedia.org)
It is important to note here that the developing dorsal and ventral buds are characterized as endoderm, and it isn't until the expression of Pdx1 (specification of endoderm to a stem cell multipotent state) that the endoderm to pancreatic progenitor transition occurs. (wikipedia.org)
Stem Cell Reports , 10 (3), 712-724. (prinsesmaximacentrum.nl)
A potassium inward rectifier (K(it)) current was previously shown by us to be induced in primitive hematopoietic progenitor cells, stimulated with the combination of interleukin-3 (IL-3) and stem cell factor (SCF). (bgu.ac.il)
Demyelinating conditions are difficult to treat and stem cell therapy using bone marrow mesenchymal stem cells (BMSCs) is a promising novel strategy. (biomedcentral.com)
The terms "progenitor cell" and "stem cell" are sometimes equated. (phys.org)
Hematopoiesis is an important model for stem cell differentiation with great medical significance. (europa.eu)
Importantly, the lab was the first to demonstrate liver engraftment of stem cell-derived cholangiocytes in vivo, setting the stage for cell-based therapeutic options for the cholangiopathies. (mayo.edu)
Fuchs, E. The tortoise and the hair: slow-cycling cells in the stem cell race. (nature.com)
Defining the epithelial stem cell niche in skin. (nature.com)
For pediatric patients with refractory cytopenia, certain cytogenetic abnormalities, or malignant transformation, hematopoietic stem cell transplantation (HSCT) from a matched related or unrelated donor early in the course of the disease is the treatment of choice. (medscape.com)
Aberration occurs in a stem cell that can give rise to multiple lineages. (medscape.com)
My group works with translational stem cell biology. (lu.se)
Learn about Stem-PD and how a transplant of stem cell-derived nerve cells was administered to a person with Parkinson's at Skåne University Hospital, Sweden. (lu.se)
Develop a stem cell-based therapy for Parkinson's Disease. (lu.se)
The true opportunity of this work lies in the potential to deliver patient-specific treatments as well as curative stem cell-based therapies to patients globally. (lu.se)
Listen to a podcast where Malin Parmar talks about her study published in Cell Stem Cell. (lu.se)

abstract = "Generating an unlimited source of human insulin-producing cells is a prerequisite to advance β cell replacement therapy for diabetes. (prinsesmaximacentrum.nl)
abstract = "Chondrogenic differentiation by mesenchymal progenitor cells (MPCs) is associated with cytokines such as transforming growth factor-beta 1 (TGF-β1) and dexamethasone. (tmu.edu.tw)

It was also found that the expression of the BCL-XL gene was higher during the maturation process of erythrocyte progenitor cells than during their replication process. (sciencedaily.com)
A metabolic switch from glucose to fatty acid oxidation occurs during cardiac development and cardiomyocyte maturation, however in vitro differentiation protocols do not consider the lack of fatty acids in cell culture media. (ox.ac.uk)
Addition of oleic acid stimulated the peroxisome proliferator-activated receptor alpha pathway and led to maturation of the cardiac progenitors, both before and after transforming growth factor-beta 1 differentiation. (ox.ac.uk)
Harnessing the oxidative metabolic switch induced maturation of differentiated endogenous stem cells. (ox.ac.uk)

erythroid cells after in vitro differentiation, despite having similar frequencies of phenotypic HSC initially. (lu.se)

Is Adipocyte Differentiation the Default Lineage for Mesenchymal Stem/Progenitor Cells after Loss of Mechanical Loading? (scirp.org)
The origins and basis for these fat deposits are largely unknown, but there is a possibility that the altered mechanical and biological environments lead to dysregulation of MSC/MPC and progression to preferential differentiation towards the adipocyte lineage. (scirp.org)
Hart, D. (2014) Is Adipocyte Differentiation the Default Lineage for Mesenchymal Stem/Progenitor Cells after Loss of Mechanical Loading? (scirp.org)
Hematopoietic stem/progenitor cells (HSPC) are known to be functionally diverse in terms of their self-renewal potential and lineage output. (lu.se)
Expression of non-cardiac lineage was low in general, and similar between Pre- and Post-CS cells. (unimib.it)
Genetic lineage tracing experiments have been performed by various research groups to show that the cell clusters originating from the developing foregut express a transcription factor called PDX1 (Pancreatic and duodenal homeobox 1). (wikipedia.org)
Endocrine lineage markers were detected upon in vitro differentiation. (prinsesmaximacentrum.nl)
Heterogeneity within hematopoietic progenitor populations has considerably limited characterization and molecular understanding of lineage commitment in both health and disease. (europa.eu)
The Notch signaling pathway is involved in several lineage commitment and differentiation events. (lu.se)

Here, we utilized microarrayed patterns to provide a controlled biochemical and biomechanical microenvironment for mouse liver progenitor cell differentiation. (elifesciences.org)
Liver progenitor cell activation and differentiation. (mayo.edu)
Dr. Huebert's lab studies the molecular mechanisms that coordinate the activation and differentiation of liver progenitor cells toward cholangiocytes, which involves various developmental morphogens and epigenetic modifiers. (mayo.edu)

Sources of exogenous stem/progenitor cells that are currently under evaluation in the context of acute lung injury repair include embryonic stem cells, bone marrow- or fat-derived mesenchymal stem cells, circulating endothelial progenitors, and, recently, amniotic fluid stem/progenitor cells. (nih.gov)
In the present study, we demonstrate that functional CD34 + progenitor cells can be efficiently produced from human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) by combined modulation of 2 signaling pathways. (ashpublications.org)
A potentially pre-clinical aspect of this thesis is detailed in paper №4 where I describe a robust protocol for the generation of functional mesDA neurons from human embryonic stem cells that are functional in a rat model of PD. (lu.se)

Hematopoietic stem cells (HSC) give rise to all types of blood lineages, including red blood cells (RBC). (lu.se)
Although studies have identified unique transcription factors in the selection of normal pancreatic lineages, the cells of origin and molecular mechanisms arbitrating PDAC growth are poorly defined. (amegroups.org)
We will pursue the following aims: (i) Generate a refined model of hematopoiesis by comprehensive single-cell RNA-Seq profiling of hematopoietic progenitors, (ii) validate the predicted model by in vivo functional developmental assays and then (iii) test candidate transcription and chromatin factors uncovered by our model for their role in controlling progression towards various lineages using single-cell measurements combined with CRISPR screens. (europa.eu)
This event explains the presence of multiple derangements observed in the bone marrow that involve several cell lineages. (medscape.com)

The aryl hydrocarbon receptor directs hematopoietic progenitor cell expansion and differentiation. (umassmed.edu)
We successfully isolated small percentage of CD117+ cells in amniotic fluid followed by in vitro expansion and differentiation towards nephron progenitor cells (NPCs) using well defined media and growth factors, initially differentiated cells were spindle shaped and showed fibroblastic appearance later at stage of nephron progenitors it attained the shape of rounded big clusters, differentiated cells stained positive for WT1 and negative for cluster of differentiation (CD117). (org.pk)

This protocol is used to answer key questions related to the function of human adipose progenitors in the vascular microenvironment. (jove.com)
Phenotype switching: plasticity and/or differentiation of stromal cells and their progenitors within the tumour microenvironment regulate tumour fate. (europa.eu)
Previous studies demonstrated that MSCs could ameliorate the pathology associated with ischemic heart disease, ischemic stroke, and peripheral vascular disease by releasing several critical factors that increase cell survival, provide an appropriate microenvironment for repairing damaged tissue, and induce neovascularization [ 3 - 5 ]. (hindawi.com)

The pellet is the stromal vascular fraction containing endothelial cells, immune cells, blood cells and progenitor cells. (jove.com)
These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)
Approach and Results We evaluated gene expression profiles of cord blood-derived EPCs and endothelial cells to identify the key factors in EPCendothelial cell differentiation and to show that transforming growth factor- family members contribute to EPC differentiation. (kist.re.kr)

Ando, W., Heard, B., Nakamura, N., Frank, C.B. and Hart, D.A. (2012) Ovine Synovial Membrane-Derived Mescenchymal Progenitor Cells Retain the Phenotype of the Original Tissue That Was Exposed to in Vivo Inflammation: Evidence for a Suppressed Chondrogenic Differentiation Potential of the Cells. (scirp.org)
In this study we asked whether global epigenetic mechanisms regulate timing of progenitor cell differentiation into myelin-forming oligodendrocytes in vivo. (rupress.org)
Expression of EBF2 in the PDX1 and E-cadherin-expressing and organoid forming pancreatic epithelial CSCs indeed induced ductal and acinar-like structural differentiation in vivo in tumors resulting in tumor suppression. (amegroups.org)
In the context of β cell replacement therapy for diabetes, de Koning and colleagues describe a 3D culture platform that supports ex vivo expansion of human pancreatic tissue as organoids. (prinsesmaximacentrum.nl)
Hopes for the creation of a more stable transfusion system have centered on the establishment of a technology for large-scale erythrocyte production ex vivo, using for instance iPS cells or ES cells. (sciencedaily.com)

Consequently, investigation of molecular heterogeneity in the differentiation potential of HSPC is vital to identify novel regulators that affect generation of specific cell types, especially RBC. (lu.se)

Adult stem/progenitor cells were isolated from the liver of mice with chemically induced liver injury. (elsevierpure.com)
Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
Residual pools of adult stem cells are hypothesized to be the source of lung regeneration and repair. (nih.gov)
Here, we describe a 3D culture system that supports the expansion of adult human pancreatic tissue and the generation of a cell subpopulation with progenitor characteristics. (prinsesmaximacentrum.nl)
Interestingly, gene expression profiling revealed that ALDH hi cells are closer to human fetal pancreatic tissue compared with adult pancreatic tissue. (prinsesmaximacentrum.nl)
We conclude that adult human pancreatic tissue has potential for expansion into 3D structures harboring progenitor cells with endocrine differentiation potential. (prinsesmaximacentrum.nl)
Metabolic flux analyses to assess the differentiation of adult cardiac progenitors after fatty acid supplementation. (ox.ac.uk)
The aim of this study was to assess the effect of this metabolic switch on control and differentiated adult cardiac progenitors, by fatty acid supplementation. (ox.ac.uk)
Accepted: 24 October 2012 cell yield data, our models require that adult DN1 cells divide multiple times before becoming competent to progress into DN2 stage. (lu.se)
The adult human airway epithelium is comprised of four major cell types, basal, club, goblet, and ciliated cells. (cdc.gov)

However, the frequency of functional differentiation in CSs and the role of cell clustering in supporting it remain to be established. (unimib.it)
To define whether ECM plays a functional role in regulation of the chondrogenic differentiation by MPCs, an in vitro model was used. (tmu.edu.tw)
Altogether, these results indicate that type I and II collagen, in addition to the cytokine effect, may play a functional role in regulating of chondrogenic differentiation by MPCs. (tmu.edu.tw)
Deep sequencing followed by abundancy ranking pinpointed six top hit genes potentially associated with either improved or impaired endodermal differentiation, which were selected for functional validation in CRISPR-Cas9 mediated knockout (KO) lines. (mdpi.com)
Differentiation of human pluripotent stem cells (hPSCs) into functional cell types is a crucial step in cell therapy. (ashpublications.org)
Our results suggest that combined modulation of signaling pathways may be an efficient means of differentiating hPSCs into functional CD34 + progenitor cells. (ashpublications.org)
Here, we propose to uncover the hierarchy and regulatory mechanisms controlling hematopoiesis by combining comprehensive single-cell RNA-Seq analyses, modelling approaches, advanced functional assays, single-cell CRISPR screens, knockout models and epigenetic profiling. (europa.eu)

Therefore, confirming the successful isolation and differentiation of amniotic fluid stem cells towards nephron progenitors. (org.pk)
To the best of our knowledge this is the first study from the country on the use of Amniotic fluid stem cells and their differentiation towards nephron progenitors that can be used as substitution source of cell therapy for exploration of renal diseases at cellular and molecular level and potential regenerative medicine applications. (org.pk)
Jabeen H, Wahid M, AinUddin JA, Mustafa F. Differentiation of CD117+ Amniotic Fluid Stem Cells towards Nephron Progenitors. (org.pk)

Effects of autophagy, p62, and related signaling pathways on hepatic differentiation were investigated by silencing the genes for autophagy protein 5 (ATG5) and/or SQSTM1/p62 using small interfering RNAs. (elsevierpure.com)
The induction of neuron-like differentiation is believed to be regulated by the expression of GSK-3β and Wnt/β-catenin signaling pathways. (elsevierpure.com)
This variation in the development of islet cell subtypes is due to differential gene expression and induction pathways of progenitor cells. (wikipedia.org)
How are signaling pathways coordinated together so that different organs can be produced from the common foregut progenitor? (cincinnatichildrens.org)

Mesenchymal stem/progenitor cells (MSC/MPC) are found in many tissues and fluids including bone marrow, adipose tissues, muscle, synovial membranes, synovial fluid, and blood. (scirp.org)
Borlongan, C.V., Glover, L.E., Tajiri, N., Kaneko, Y. and Freeman, T.B. (2011) The Great Migration of Bone Marrow-Derived Stem Cells towards the Ischemic Brain: Therapeutic Implications for Stroke and Other Neurological Disorders. (scirp.org)
The expression levels of activin receptor-like kinase 1 (ALK1) and its high-affinity ligand, bone morphogenetic protein 9 (BMP9) were markedly changed in EPCendothelial cell differentiation. (kist.re.kr)
A higher proportion of CD34 + cells (∼ 20%) could be derived from hPSCs by inhibition of mitogen-activated protein kinase (MAPK) extracellular signal-regulated protein kinase (MEK)/extracellular signal-regulated kinase (ERK) signaling and activation of bone morphogenic protein-4 (BMP4) signaling. (ashpublications.org)
Peripheral blood contamination in human bone marrow aspirates was calculated from the ratios between the erythrocyte and nucleated cell counts in both the bone marrow aspirate and a simultaneously obtained peripheral blood sample. (karger.com)
As the affected cell lines continue to divide and to provide the marrow with dysplastic cells, bone marrow dysfunction becomes apparent. (medscape.com)
The human bone morphogenet ic protein (rhBMP) developed by genetic engineering, was isolated by Urist, in 1965, and it is considered a substance capable of inducing differentiation of mesenchymal stem cells into osteoblasts, the cells that are responsible for the synthesis of bone matrix. (bvsalud.org)

citation needed] Pdx1 is accepted as the earliest marker for pancreatic differentiation. (wikipedia.org)
Here, we employed a human pancreatic differentiation platform complemented with an shRNA screen in human pluripotent stem cells (PSCs) to identify potential drivers of early endoderm and pancreatic development. (mdpi.com)

In addition, the laboratory has developed a novel method for the derivation of the epithelial cells that line the bile ducts (cholangiocytes) from induced pluripotent stem cells (iPSCs). (mayo.edu)
Dr. Huebert's laboratory pioneered a novel technology to create cholangiocytes from pluripotent stem cells. (mayo.edu)
No evidence of tumour formation was observed in the transplanted animals, a major concern when utilising a pluripotent cell source. (lu.se)

During the first 10 postnatal days, administration of valproic acid (VPA), the specific inhibitor for histone deacetylase activity, resulted in significant hypomyelination with delayed expression of late differentiation markers and retained expression of progenitor markers. (rupress.org)
Hepatic differentiation was assessed based on increased albumin and hepatocyte nuclear factor 4α, as hepatocyte markers, and decreased cytokeratin 19 and SOX9, as stem/progenitor cell markers. (elsevierpure.com)
The aim of our study is to quantify differentiation of a muscle-type Ca(2+) release mechanism in CS-derived cells, correlate it with cardiac differentiation markers and test its dependency on CS formation. (unimib.it)
These cells display high aldehyde dehydrogenase activity (ALDH hi ), express pancreatic progenitors markers (PDX1, PTF1A, CPA1, and MYC), and can form new organoids in contrast to ALDH lo cells. (prinsesmaximacentrum.nl)
Optimal differentiation was verified by qPCR and immunofluorescent (IF) staining of cell-type specific markers. (cdc.gov)
Cells were harvested at 24 hours or 7 days post exposure, to investigate changes in (a) inflammation, (b) oxidative stress-related, and (c) cell-type specific markers using qPCR and/or IF staining. (cdc.gov)
Results: qPCR analysis of the differentiation time-course shows a steady increase in markers of terminally differentiated cells, such as club (SCGB1A1), goblet (MUC5AC) and ciliated cells (FoxJ1). (cdc.gov)
Exposure to high dose of nZnO induced a significant decrease in expression of club (SCGB1A1), goblet (MUC5AC) and ciliated cells (FoxJ1) markers at mRNA levels. (cdc.gov)

Recently, we reported that retrovirus-delivered human arginine decarboxylase (hADC) genes improve cell survival against oxidative insult in murine NPCs in vitro. (elsevierpure.com)

hPSC-derived CD34 + progenitor cells further developed to endothelial and smooth muscle cells with functionality. (ashpublications.org)
Employing IL-3 + SCF stimulated human cord blood CD34 + 38 - cells, we isolated and characterized different components of this current. (bgu.ac.il)
Inhibition of the expression of each of the channels suppressed progenitor cell generation by IL-3 and SCF-stimulated CD34 + 38 - cells in 7- day suspension cultures. (bgu.ac.il)

The expressional changes of Hes1, a transcription factor negatively regulating OPC-differentiation, were studied. (biomedcentral.com)

Mnx1 expression has been shown to be important for the development of dorsal Pdx1, hence acting as a necessary transcription factor for the specification of foregut endoderm into Pdx1 expressing pancreatic progenitors. (wikipedia.org)
In particular, we study the foregut progenitor cells in the ventral endoderm, which are the early building blocks of the liver, lungs and pancreas. (cincinnatichildrens.org)

At least two populations of epithelial stem/progenitor cells give rise to the lung anlage, comprising the laryngo-tracheal complex versus the distal lung below the first bronchial bifurcation. (nih.gov)
These cells have been shown to have 28 genes regulating the cell cycle to be upregulated, showing that they are proliferative cells having the ability to replace and give rise to multiple cell populations in the pancreas. (wikipedia.org)

The identification of mechanisms modulating timing of cellular differentiation is critical for morphogenesis and proper development. (rupress.org)
This effect was observed at both 24 hours and 7 days' time points, suggesting an early blockage in the cellular differentiation process, which requires further evaluation. (cdc.gov)

Batsali, A.K., Kastrinaki, M.C., Papadaki, H.A. and Pontikoglou, C. (2013) Mesenchymal Stem Cells Derived from Wharton's Jelly of the Umbilical Cord: Biological Properties and Emerging Clinical Applications. (scirp.org)

In this study, we examined effects of autophagy, p62, and associated signaling on hepatic differentiation. (elsevierpure.com)
Inhibition of autophagy promoted hepatic differentiation in the stem/progenitor cells. (elsevierpure.com)
Conversely, SQSTM1/p62 silencing impaired hepatic differentiation. (elsevierpure.com)
A suggested mechanism for p62-dependent hepatic differentiation in our study was activation of the mTOR pathway by amino acids. (elsevierpure.com)
Our findings indicated that promoting amino acid sensitivity of the mTOR pathway is dependent on p62 accumulated by inhibition of autophagy and that this process plays an important role in the hepatic differentiation of stem/progenitor cells. (elsevierpure.com)
The differential of pancreatic progenitors from hepatic progenitors is also notable, as Hhex1 (Hematopoietically expressed homeobox gene) is responsible for the origination of pancreatic progenitor cells. (wikipedia.org)

After centrifugation the cell pellet was treated for analysis of CD117+ cells using flowcytometry, once small percentage of CD117+cells were identified the cells were prepared for differentiation and that was carried out using specific growth factors including BMP4, BMP7, FGF2, and retinoic acid, providing the niche to the stem cells for differentiation towards nephron progenitors which was confirmed by protein expression of Wilms Tumor-1 (WT1) using immunofluorescence analysis. (org.pk)
In this study, we investigated how higher-order chromatin structure modulates differential expression of the human INK4b-ARF-INK4a locus during progenitor cell differentiation, cellular ageing and senescence of cancer cells. (biomedcentral.com)
Expression of the INK4b-ARF-INKa locus during cellular ageing and differentiation . (biomedcentral.com)
Each distinct CD4 + T-cell subset has a specific function and can mainly be distinguished by its cytokine profile, the expression pattern of cell surface molecules, and the activation of specific transcription factors 8 . (nature.com)
This study investigates whether the induced expression of hADC gene in mNPCs induces any significant change in the cell fate commitment. (elsevierpure.com)
Pancreatic progenitors have been shown to arise from the early expression of the gene Mnx1/Hlxb1 (Motor Neuron and pancreas homobox 1). (wikipedia.org)
Hence the expression of this protein also contributes to the formation of these pancreatic progenitors expressing Pdx1. (wikipedia.org)
The research showed that an increase in the expression of the c-MYC gene led to an enhancement of the replication ability of erythrocyte progenitor cells. (sciencedaily.com)
Based on a previous report, we studied the potential involvement of the adenosine A1 receptor in the effect of CBC on these cells and found that the selective adenosine A1 receptor antagonist, DPCPX, counteracted both ERK1/2 phosphorylation and up-regulation of nestin by CBC, indicating that also adenosine is involved in these effects of CBC, but possibly not in CBC inhibitory effect on GFAP expression. (nih.gov)
Lentivirus-mediated expression of SPDEF in hSABCi-NS1.1 cells induced secretory cell metaplasia, accompanied with characteristic COPD-associated SAE secretory cell changes, including up-regulation of MSMB, CEACAM5 and down-regulation of LTF. (biomedcentral.com)
Reverse transcription-polymerase chain reaction (RT-PCR) subcloning identified the expression of a strongly rectifying K(ir) channel (K(ir) 4.3) as well as a weakly rectifying K(ir) channel (K(ir) 1.1) in these cells. (bgu.ac.il)
The variable expression of two essential inward rectifying potassium channels early in the course of hematopoietic progenitor cell differentiation may play a potentially important role in potassium homeostasis in these cells. (bgu.ac.il)
In vitro cultured OPCs showed more differentiation after incubation with BMSC-CM. Hes1 expression was found to be significantly increased by HD and decreased by BMSC or BMSC-CM. The change of Hes1 was found, however, independent of Notch1 activation, but dependent on TNFα/RelB signaling. (biomedcentral.com)
Addition of oleic acid following differentiation increased expression of myosin heavy chain 7 and connexin 43. (ox.ac.uk)
The basal cell (stem/progenitor cell) marker (KRT5) shows an initial increase in expression, but did not vary much during differentiation. (cdc.gov)
In parallel, the expression of basal cell marker(KRT5) was partially modulated by nZnO exposure. (cdc.gov)

Use of human fetal tissue raises several ethical issues, but are there alternative cell sources that can substitute effectively? (lu.se)

Different phenotypes and subtypes of endothelial progenitor cells (EPCs), such as early and late EPCs, have been described according to their functionality. (intechopen.com)
Although differentiation of naïve CD4 + T cells has been considered to be an irreversible process, accumulating evidence suggests that T cells are much more flexible than initially believed with the ability to interchange between helper phenotypes 15 . (nature.com)

The mechanism of action is similar to that of endogenous erythropoietin, which interacts with stem cells to increase red blood cell production. (medscape.com)

This is due to modulation by adhesive interactions with stroma, stroma-derived ECM or cytokines bound to the membrane of stromal cells, rather than resulting from the production of diffusible cytokines by stromal cells. (amsterdamumc.org)

Strategies to enhance endogenous alveolar epithelial repair could include protection of epithelial progenitors from injury and/or stimulation of endogenous progenitor cell function. (nih.gov)

The resulting cell line (hSABCi-NS1.1) was characterized by RNAseq, TaqMan PCR, protein immunofluorescence, differentiation capacity on an air-liquid interface (ALI) culture, transepithelial electrical resistance (TEER), airway region-associated features and response to genetic modification with SPDEF. (biomedcentral.com)

CD4 + T cells are involved in the development of autoimmunity, including multiple sclerosis (MS). Here we show that nicotinamide adenine dinucleotide (NAD + ) blocks experimental autoimmune encephalomyelitis (EAE), a mouse model of MS, by inducing immune homeostasis through CD4 + IFNγ + IL-10 + T cells and reverses disease progression by restoring tissue integrity via remyelination and neuroregeneration. (nature.com)

Transplantation of midbrain dopaminergic progenitor cells is a promising treatment for Parkinson's disease . (bvsalud.org)
Here, we developed a one-step biomanufacturing platform that uses small-aperture gelatin microcarriers to produce beads carrying midbrain dopaminergic progenitor cells . (bvsalud.org)
These beads allow midbrain dopaminergic progenitor cell differentiation and cryopreservation without digestion , effectively maintaining axonal integrity in vitro . (bvsalud.org)
This involves transplantation of developing midbrain cells from aborted fetuses, (the part that form mesDA neurons), into the striatum of a PD patient. (lu.se)

In addition to the established roles of TGFβ and Notch signaling in this fate specification process, there is increasing evidence that liver progenitors are sensitive to mechanical cues. (elifesciences.org)
We have therefore identified distinct combinations of microenvironmental cues which guide fate specification of mouse liver progenitors toward both hepatocyte and biliary fates. (elifesciences.org)
Morris, R. J. & Potten, C. S. Highly persistent label-retaining cells in the hair follicles of mice and their fate following induction of anagen. (nature.com)
One of these is fate determination of the common lymphoid progenitor, promoting T-cell development at the expense of B-cell differentiation. (lu.se)

CRT aims to replace neurons that have degenerated in PD, with donor cells that have the potential to functionally re-integrate into the host circuitry. (lu.se)
Our current focus is to learn how to direct and efficiently drive controlled differentiation of human stem cells into subtype-specific neurons. (lu.se)

It is essential to have sensitive and reproducible methods of either quantifying or isolating these stem cells and progenitors to understand their intrinsic properties and how extrinsic signals regulate their development. (karger.com)
Notch signaling has been shown to regulate the number of exocrine and endocrine cells in the pancreas, but not without the presence of Pdx1. (wikipedia.org)

Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)
Objective Modulating endothelial progenitor cells (EPCs) is essential for therapeutic angiogenesis, and thus various clinical trials involving EPCs are ongoing. (kist.re.kr)
Interestingly, BMP9 induced EPCendothelial cell differentiation and EPC incorporation into vessel-like structures by acting on ALK1 expressed on EPCs in vitro. (kist.re.kr)
We studied the secretome profile of gestational tissue-derived MSCs and their effects on functions of endothelial progenitor cells (EPCs), another angiogenic cell type that plays an important role during the neovascularization. (hindawi.com)
However, recent studies have demonstrated that other angiogenic cells called endothelial progenitor cells (EPCs) also play an important role during the formation of new vessels through the process of vasculogenesis [ 7 - 9 ]. (hindawi.com)

We found that INK4b and INK4a , but not ARF , are upregulated following the differentiation of haematopoietic progenitor cells, in ageing fibroblasts and in senescing malignant rhabdoid tumour cells. (biomedcentral.com)

DSpace at KIST: BMP9 Induces Cord Blood-Derived Endothelial Progenitor Cell Differentiation and Ischemic Neovascularization via ALK1. (kist.re.kr)

Mitochondrial reactive oxygen species promote epidermal differentiation and hair follicle development. (nature.com)
The term organoid nevus may be used to emphasize the admixture of epidermal cells often evident in individual lesions of epidermal nevi. (medscape.com)

When we chemically modified two main signaling elements in this pathway, adenylyl cyclase (AC) and phosphodiesterase (PDE), we observed that inhibition of PDE led to 10 times higher yield of Glycophorin-A + cells than activation of AC. (lu.se)
Notch signaling allows the expansion of pancreatic progenitors by the process of lateral inhibition. (wikipedia.org)

Here we show that the brown adipose differentiation gene, EBF2, activates differentiation-associated signals in the cancer progenitor cells in culture and CSC transplantation models. (amegroups.org)
As the c-MYC gene and BCL-XL gene are present within erythrocyte progenitor cells, methods that make use of them actually mimic the body's own system and can therefore be thought of as safer with a view to future clinical application than the previously developed systems for large-scale erythrocyte production using oncogene-derived from Human papillomavirus that are not normally expressed in the human body. (sciencedaily.com)

Pancreatic progenitor cells have been shown to arise from cells originating from the developing foregut during mammalian development. (wikipedia.org)

A significantly lower number of megakaryocytes (CD41- and CD61-positive cells) was obtained from contact cultures compared to non-contact cultures. (amsterdamumc.org)

Pdx1 has been shown to be a marker for all pancreatic and mid-gut progenitor cells. (wikipedia.org)

Anatomy67

Organisms11

Diseases7

Chemicals and Drugs83

Analytical, Diagnostic and Therapeutic Techniques and Equipment35

Phenomena and Processes55

Disciplines and Occupations1

Technology, Industry, Agriculture1

Information Science3

Neural14

Hematopoietic stem and progenitor cells3

Differentiate18

Cardiac progenitor cells3

Proliferation14

Oligodendrocyte progenitor1

Neuronal9

Regulates4

Stem Cell23

Abstract2

Maturation4

Erythroid cells1

Lineage9

Liver progenitor3

Embryonic Stem3

Lineages4

Expansion and differentiation2

Microenvironment3

Endothelial cells3

Vivo5

Heterogeneity1

Adult10

Functional7

Amniotic fluid3

Pathways4

Bone7

Pancreatic differentiation2

Pluripotent3

Markers8

NPCs1

CD343

Negatively1

Endoderm2

Populations2

Cellular differentiation2

Mesenchymal stem cells de1

Hepatic6

Expression16

Fetal1

Phenotypes2

Mechanism1

Stromal1

Endogenous1

Genetic1

Homeostasis1

Midbrain4

Fate4

Neurons2

Regulate2

EPCs5

Haematopoietic1

Cord blood1

Epidermal2

Inhibition2

Gene2

Mammalian1

Megakaryocytes1

PDX11