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CalmodulinTrifluoperazineCalmodulin-Binding ProteinsCalciumMolecular Sequence DataAmino Acid SequenceCalcium-Binding ProteinsMyosin-Light-Chain KinaseKineticsCalcium-Calmodulin-Dependent Protein Kinase Type 2Protein BindingCattleBinding SitesBiological ProductsSulfonamidesBase SequenceCalcium-Calmodulin-Dependent Protein KinasesEgtazic Acid2',3'-Cyclic-Nucleotide PhosphodiesterasesCalcium-Transporting ATPasesChlorpromazineEnzyme ActivationPhosphorylationCloning, Molecular3',5'-Cyclic-AMP PhosphodiesterasesSesterterpenesNeurograninMolecular WeightElectrophoresis, Polyacrylamide GelMelittenCyclic Nucleotide Phosphodiesterases, Type 1Peptide FragmentsSequence Homology, Amino AcidProtein Structure, TertiaryMutationProtein ConformationRecombinant ProteinsRabbitsCalcineurinChickensBrainPhenothiazinesCalcium-Calmodulin-Dependent Protein Kinase Type 1Cell LineProtein KinasesPeptidesCa(2+) Mg(2+)-ATPaseParameciumSubstrate SpecificityEscherichia coliWasp VenomsEF Hand MotifsPrenylamineChromatography, AffinityDairy ProductsPhosphoric Diester HydrolasesSpectrometry, FluorescenceModels, MolecularPhosphorylase KinaseCells, CulturedCalcium SignalingGizzardTroponinCell MembraneChromatography, High Pressure LiquidMagnetic Resonance SpectroscopyBenzylaminesRNA, MessengerMyosinsConsumer Product SafetyTrypsinFluphenazinePlasmidsTroponin CSignal TransductionRecombinant Fusion ProteinsPhosphoprotein PhosphatasesActinsSequence AlignmentEnzyme InhibitorsMyosin Type IAdenylate CyclaseGlycosylation End Products, AdvancedAmino AcidsProtein Kinase CBee VenomsErythrocyte MembraneMutagenesis, Site-DirectedMagnesiumStructure-Activity RelationshipCalcimycinDNA, ComplementaryHydrogen-Ion ConcentrationMembrane ProteinsTime FactorsDose-Response Relationship, Drug
AntibodySensor and signal transducerProtein kinasesProteinsRegulatesPhosphorylationBindsPeptideInhibitsGeneRecombinant proteinELISA KitInhibitorMutations1983BioactiveTropomyosinInteractionsCalcium-dependentCytosolicKinaseDetailsHumanTargetDescriptionSubstratePurityCitationsCartRegulationApplicationsTocrisFunctionsTypeReferencesVialFamilyFoundSUPPORTCompoundsPublication
Antibody5
  • The following product was used in this experiment: Phospho-Calmodulin 1/2/3 (Thr79, Ser81) Polyclonal Antibody from Thermo Fisher Scientific, catalog # PA5-104932, RRID AB_2816405. (thermofisher.com)
  • If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase. (thermofisher.com)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Calmodulin Like Protein 5 (CALML5) in Tissue homogenates, cell lysates and other biological fluids. (glideruniversity.org)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Calmodulin Like Protein 5 (CALML5) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (glideruniversity.org)
  • The following product was used in this experiment: Phospho-CaMKII alpha/beta/delta (Tyr231) Polyclonal Antibody from Thermo Fisher Scientific, catalog # PA5-17108, RRID AB_10987028. (thermofisher.com)
Sensor and signal transducer1
Protein kinases1
  • The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. (thermofisher.com)
Proteins8
  • This gene encodes one of three calmodulin proteins which are members of the EF-hand calcium-binding protein family. (nih.gov)
  • As it binds calcium, calmodulin undergoes conformational changes which can increase its affinity for target proteins. (thermofisher.com)
  • Two teams presented products to rapidly and effectively purify proteins commonly used in research, which could be sold to commercial or academic laboratories. (brandeis.edu)
  • Calmodulin is an important multifunctional molecule that regulates the activities of a large number of proteins in the cell. (biomedcentral.com)
  • Calcium binding induces conformational transitions in calmodulin that make it specifically active to particular target proteins. (biomedcentral.com)
  • Medicago's bioactive molecule products - defined as compounds that have an effect on a living organism, tissue or cell - include bioactive proteins such as Protein A and Calmodulin, as well as saponin. (medicagogroup.com)
  • TOCP administration conferred calcium and calmodulin dependence on the phosphorylation of a few brain cytosolic proteins and caused an increase in the phosphorylation of a number of other cytosolic and membrane proteins. (cdc.gov)
  • The effect of TOCP intoxication on in-vitro phosphorylation of brain and spinal cord synaptosomal proteins appeared to follow the time course as described in earlier studies for the morphological appearance of neuronal breakdown products in the nerve endings. (cdc.gov)
Regulates1
  • Calcium-induced activation of calmodulin regulates and modulates the function of cardiac ion channels. (nih.gov)
Phosphorylation3
  • A synthesized peptide derived from human Calmodulin around the phosphorylation site of Thr79/Ser81. (thermofisher.com)
  • In this study, we revealed that Homer3 was phosphorylated by calcium/calmodulin-dependent protein kinase II (CaMKII) in Purkinje cells, and the phosphorylation reduces the affinity for Homer target molecules, resulting in change of the solubility. (jneurosci.org)
  • All subtypes are closely associated with calmodulin and FK506-binding protein and are modulated through phosphorylation by PKA, PKC, PKG and CaMKII. (novusbio.com)
Binds1
  • Other choices include calmodulin-binding peptide, Strep-tag (oxtapeptide binding to streptavidini), chitin-binding domain, and FLAG-tag (another octapeptide that binds to anti-FLAG mAbs). (genengnews.com)
Peptide1
  • Synthetic peptide corresponding to aa 281-294 (M 281 HRQET(PSO 3 )VDCLKKFN 294 ) of the α-subunit of mouse and rat CaM kinase II (Ca 2+ /Calmodulin-dependent Protein Kinase II) thiophosphorylated at (Thr 286 ). (enzolifesciences.com)
Inhibits2
  • Calmodulin antagonist (inhibits calmodulin activated PDE activity with an IC50 of 68 μM). (fishersci.com)
  • W-5 hydrochloride is a calmodulin antagonist (inhibits Ca 2+ -calmodulin dependent PDE with an IC 50 of 240 μ M). (tocris.com)
Gene2
  • The product of this gene belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. (genetex.com)
  • GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies ( Molecular Function , Biological Process , and Cellular Component ), a reference, and an evidence code. (yeastgenome.org)
Recombinant protein1
  • Graduate students from Assistant Professor of Biology Michael T. Marr 's lab presented a tool to purify Calmodulin-tagged recombinant protein. (brandeis.edu)
ELISA Kit1
  • Description: A sandwich ELISA kit for detection of Calmodulin Like Protein 5 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (glideruniversity.org)
Inhibitor2
  • Cotreatment with ROCK inhibitor Y-27632 [4-(1-aminoethyl)- N -(4-pyridyl) cyclohexanecarboxamide dihydrochloride] and MLCK activator calmodulin reduced bile canaliculi constriction and dilatation, respectively, confirming the role of these pathways in drug-induced intrahepatic cholestasis. (aspetjournals.org)
  • We provide evidence that KRas translocation occurs through sequestration of the polybasic-prenyl motif by Ca 2+ /calmodulin (Ca 2+ /CaM) and subsequent release of KRas from the PM, in a process reminiscent of GDP dissociation inhibitor-mediated membrane recycling of Rab and Rho GTPases. (rupress.org)
Mutations2
19831
Bioactive2
Tropomyosin1
  • Calponin a 34 kDa polypeptide, is a cytoskeleton-associated actin-binding protein that also interacts tropomyosin and calmodulin. (biocare.net)
Interactions3
  • Calmodulin Interactions with Voltage-Gated Sodium Channels. (nih.gov)
  • In this study, we adopt a structural systems biology approach and develop a mathematical model to investigate various types of cooperative calcium-calmodulin interactions. (biomedcentral.com)
  • We compare the predictions of our analysis with physiological dose-response curves taken from the literature, in order to provide a quantitative comparison of the effects of different mechanisms of cooperativity on calcium-calmodulin interactions. (biomedcentral.com)
Calcium-dependent1
  • The results of our analysis reduce the gap between current understanding of intracellular calmodulin function at the structural level and physiological calcium-dependent calmodulin target activation experiments. (biomedcentral.com)
Cytosolic1
  • Calmodulin (CaM) is a cytosolic Ca 2+ -binding protein that serves as a control element for many enzymes. (nicoyalife.com)
Kinase3
  • We found that Homer3, the predominant isoform in Purkinje cells, is phosphorylated by calcium/calmodulin-dependent protein kinase II (CaMKII) both in vitro and in vivo . (jneurosci.org)
  • CaMKII is an ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+ and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. (thermofisher.com)
  • Calmodulin Binding Domain Ca2+ is a fragment of aa290-309 of Calmodulin-dependent Protein Kinase II (CaMKII). (echelon-inc.com)
Details1
  • For details and product specific hints, please refer to the "Remarks" section. (sysy.com)
Human3
Target1
  • Five teams of young Brandeis scientists and programmers will receive a total of $50,000 to fund the research and development of products ranging from compounds that can target cancer cells to a mobile app that helps avoid lines. (brandeis.edu)
Description1
  • Description: A competitive ELISA for quantitative measurement of Rat Calmodulin like protein 5(CALML5) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (glideruniversity.org)
Substrate1
  • Time-resolved DEER EPR and solid-state NMR afford kinetic and structural elucidation of substrate binding to Ca(2+)-ligated calmodulin. (nih.gov)
Purity1
  • Where yield and purity are issues, particularly in very small or dilute samples, Dr. Boronowsky recommends forgoing resins in favor of magnetic beads, which provide easier separation of the final product. (genengnews.com)
Citations2
  • Citations are publications that use Tocris products. (tocris.com)
  • In addition, citations to Web sites external to NIOSH do not constitute NIOSH endorsement of the sponsoring organizations or their programs or products. (cdc.gov)
Cart1
Regulation1
  • Studies have found that calmodulin participates in the regulation of several biological processes including energy and biosynthetic metabolism, cell motility, exocytosis, cytoskeletal assembly, and intracellular modulation of both cAMP and calcium concentrations. (thermofisher.com)
Applications2
  • Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use. (thermofisher.com)
  • All products are for research use only-Not for use in diagnostic or therapeutic applications. (genetex.com)
Tocris2
  • Tocris products are intended for laboratory research use only, unless stated otherwise. (tocris.com)
  • You can now submit reviews for your favorite Tocris products. (tocris.com)
Functions1
  • Subtype Differences in the Interaction of HIV-1 Matrix with Calmodulin: Implications for Biological Functions. (nih.gov)
Type1
  • Calmodulin variant E140G associated with long QT syndrome impairs CaMKIIδ autophosphorylation and L-type calcium channel inactivation. (nih.gov)
References1
  • References are publications that support the biological activity of the product. (tocris.com)
Vial2
  • When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online). (tocris.com)
  • For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap. (antibodiesinc.com)
Family1
  • Members of the PDE1 family are calmodulin-dependent PDEs that are stimulated by a calcium-calmodulin complex. (creativebiomart.net)
Found1
SUPPORT1
  • Guaranteed product quality, expert customer support. (abcam.com)
Compounds1
  • Graduate student Marcus Long pitched his team's life science product: A new class of compounds that can be used as research tools to understand cancer, neurodegeneration and infection and could potentially be developed into drugs. (brandeis.edu)
Publication1