Processes reaction biological assay. Medical search. Frequent questions

The most widely used is a colorimetric assay developed by Ellman et al (1961). (cdc.gov)
Linear detection range: 4.8 to 500 µM or 0.054-5.7 mg/dL (colorimetric assay) and 0.25 to 100 µM or 0.0028-1.14 mg/dL (fluorimetric assay) for a 60 min reaction. (biolisp.org)
1. Bernal C, Palacin C, Boronat A, Imperial S. (2005) A colorimetric assay for the determination of 4-diphosphocytidyl-2-C-methyl-D-erythritol 4-phosphate synthase activity. (cmscorp.co.kr)
The Nitric Oxide Detection Kit is a colorimetric assay for the indirect determination of Nitric Oxide concentrations based on the enzymatic conversion of nitrate to nitrite by a nitrate reductase. (ozbiosciences.com)

The Wampole Laboratories (Wampole) H. pylori IgG Enzyme-Linked Immunosorbent Assays (ELISA) is intended for the detection and qualitative determination of IgG antibodies to Helicobacter pylori in human serum. (cdc.gov)
Enzyme linked immunosorbent assays (ELISA) rely on the ability of biological materials, (i.e. antigens) to adsorb to plastic surfaces such as polystyrene (solid phase). (cdc.gov)
Though many traditional sensing technologies for biomarker analysis and fundamental biological studies (i.e. enzyme-linked immunosorbent assays, real-time polymerase chain reaction, etc.) rely on single-parameter measurements, it has become increasingly clear that the inherent complexity of many human illnesses and pathways necessitates quantitative and multiparameter analysis of biological samples. (illinois.edu)
In this study, we describe the effect of a single amino-acid variation on a prototypical enzyme, human carbonic anhydrase II (CA II), by correlating its high-resolution reaction-intermediate structures with the measured kinetic parameters. (iucr.org)
Recently, a fluorescent based ChE assay kit was marketed for measurement of the purified enzyme (A12217, Molecular Probes, Eugene, OR, USA ( Zhou et al 2000). (cdc.gov)
Enzyme-linked immunosorbent assays (ELISA) have been around as one of the primary methods of analyte detection for more than four decades. (ddw-online.com)
Samples: Enzyme reactions with free phosphate releases. (wlsolutions.be)
This PhosphoWorks Phosphate Assay Kit has been developed for measuring the activity of any Pi-generating enzyme. (cmscorp.co.kr)
An addition to the existing requirements was made by introducing a new molecular technique, called mutant analysis by polymerase chain reaction and restriction enzyme cleavage (MAPREC), for the quality control of the vaccine. (who.int)
PhosphoWorks™ Colorimetric ATP Assay Kit is based on a serial ATP-induced enzyme coupled reactions to produce hydrogen peroxide, which is spectrophotometrically quantified with our Amplite® Red Substrate at OD 570 nm. (aatbio.com)
1. Add 5 mL of Assay Buffer (Component C) into Enzyme Mix bottle (Component B), and mix well. (aatbio.com)
Standard protein purification and characterization techniques, enzyme activity assays, as well as immunoassays (ELISA) will also be taught. (uaeu.ac.ae)

Over the years many changes to the basic format have resulted in assay improvements, but some of the most recent look set to take the traditional ELISA to new levels. (ddw-online.com)
These include many vendors now addressing the automation of ELISA assays. (ddw-online.com)
Other automated instrumentation is designed to support the creation/coating of ELISA assay plates or contains cartridge-based delivery of assay reagents to reduce set-up time. (ddw-online.com)
These are supported by a wide range of ELISA reagents, assay and kits providing relative profiling or absolute quantitation of proteins across a wide range of study areas. (ddw-online.com)
E nzyme-linked immunosorbent assays (ELISA) have been one of the primary methods of detecting the presence of a substance, usually an antigen, in an aqueous sample for more than 40 years. (ddw-online.com)
The immunoassay landscape has, however, dramatically changed in recent years with the development of reduced assay steps protocols, no wash ELISAs and new automated systems designed to run ELISA. (ddw-online.com)
This review article uses as its starting point some of the findings of HTStec's ELISA Assay Trends 2012 survey and report, published in June 2012 (1). (ddw-online.com)
This report discusses end-users' current practices and preferences in ELISA assays, and seeks to understand future user requirements. (ddw-online.com)
The main (primary) application area for survey respondents' ELISA assays was immunology (17% selecting). (ddw-online.com)
The Total Lipid Hydroperoxide ELISA Assay Kit is intended for the quantitative determination of peroxides in EDTA-plasma, serum and cell culture supernatants. (eaglebio.com)
The Total Lipid Hydroperoxide ELISA Assay kit is for research use only and not to be used in diagnostic procedures. (eaglebio.com)
The Total Lipid Hydroperoxide ELISA Assay kit allows an easy, rapid and precise quantitative determination of the total peroxides in biological samples. (eaglebio.com)
Eagle Biosciences has quickly become a leading provider of ELISA assay kits, HPLC assay kits, Molecular Biology assays, antibodies, and proteins. (eaglebio.com)
Description: A competitive ELISA for quantitative measurement of Human Anti centriole and centrosome antibody IgG in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (gentaur-online.com)
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. (gentaur-online.com)

Tests or protocols may include hazardous reagents or biological agents. (cdc.gov)
Creatinine Assay Laboratories manufactures the assay creatineng uk reagents distributed by Genprice. (biolisp.org)
The silver enhancement reagents may be integrated into the microfluidic assay platform to be released upon sample addition. (hindawi.com)
Human IgG antibody Laboratories manufactures the quantichrom ™ magnesium assay kit for approx 250 reactions manufacturer bioassay systems biotrend catalogue number dimg 250 st reagents distributed by Genprice. (gentaur-online.com)

While the Ellman assay is simple and inexpensive, the absorbance peak of hemoglobin (415nm) can interfere with detection of its reaction end-product, 2-nitro-5-benzoic acid (405nm). (cdc.gov)
P erhaps most significantly are the big increases in assay sensitivity and the widening of the dynamic range accomplished by using alternatives to the traditional colorimetric detection, or by the use of new platform technologies. (ddw-online.com)
Therefore, the accurate detection of physiological NO concentration is crucial to the understanding of NO signaling and its biological role. (intechopen.com)
Most methods used for NO detection are indirect including spectroscopic approaches such as the Griess assay for nitrite and detection of methemoglobin after NO reaction with oxyhemoglobin. (intechopen.com)
The kit is formulated to give sensitive detection of Pi, providing an alternative to hazardous radioactive methods and other less sensitive colorimetric assays. (cmscorp.co.kr)
Linear detection range from 12 to 250 µM carbonyl in 96-well plate assay. (gentaur-online.com)
Linear detection range 0.7 mg/dL (0.2 mM) to 35 mg/dL (10 mM) Cl - in 96-well plate assay. (gentaur-online.com)
Detection range 0.2 µM (7 ng/mL) to 30 µM (1,020 ng/mL) H 2 O 2 in 96-well plate assay. (gentaur-online.com)
Miniature, self-contained analysis systems, often referred to as labs-on-a-chip, have streamlined various assays, providing near-real-time results of analyses such as glucose or pathogen detection in blood products. (mit.edu)
The radioisotope filtration binding assay allows for the detection of kinase phosphoryl transfer activity, which is proportional to the amount of phosphorylated substrate. (kinasebiotech.com)
The scintillation proximity assay (SPA) using radioisotope-labeled ATP is an activity-based homogenous and versatile assay that allows for rapid and sensitive detection of kinase activity. (kinasebiotech.com)

Description: Our Phosphate Assay Kit (Fluorometric) measures total inorganic phosphate present in lysates, solutions, food, or biological samples in a 96-well fluorescence-based plate reader. (wlsolutions.be)

Screen Quest™ assay kits, a set of HTS-ready tools for high throughput screening of biochemical and cellular targets such as protein kinases, proteases, HDAC, cell apoptosis and cytoxicity, GPCR, ion channels, ADME/metabolism and transporters. (stratech.co.uk)
Can be readily automated as a high-throughput assay for thousands of samples per day. (gentaur-online.com)
SDS PAGE, filtration techniques, chromatography, or density gradient centrifugation are typically used to separate radiolabeled ATP from radiolabeled substrate in low-throughput assays, while Scintillation Proximity Assay (SPA) or FlashPlate assays can be used for high-throughput assays. (kinasebiotech.com)

Te identifier uses polymerase chain reaction for higher fidelity identification of biological samples, while the lateral flow assays can screen for bioagents of concern. (army.mil)
Using polymerase chain reaction Mycoplasma hominis and Ureaplasma urealyticum were detected in cervical secretions of 19.2% and 13.7% of infertile women, and the presence of mycoplasma was significantly correlated with the presence of AZP-Ab and ASA. (who.int)
The PMCA test is based on a prion amplification that is similar to polymerase chain reaction (PCR), a widely used test for detecting traces of DNA in different tissues. (medscape.com)

It is for 1000 assays using 96-well plates (100 ul of sample volume) or 4000 assays using 384-well plates (25 l of sample volume). (wlsolutions.be)

The Quantichrom ™ Magnesium Assay Kit For Approx 250 Reactions Manufacturer Bioassay Systems Biotrend Catalogue Number Dimg 250 St reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (gentaur-online.com)

Radiometric assays allow direct measurement of the rate of a given kinase reaction to accurately determine and quantify its catalytic activity. (kinasebiotech.com)

It provides a robust, simple and convenient assay for measuring ATP levels in biological samples. (aatbio.com)
Gel electrophoresis provides a convenient assay to detect splicing and to locate the spliced regions. (brainkart.com)

When the enzymatic reaction is stopped with 1N H 2 SO 4 , the contents of the wells turn yellow. (cdc.gov)
This task has now been successfully completed through the strategic and comprehensive process of identifying limitations of existing enzymatic bio-analysis test kits where they occurred, and then using advanced techniques, such as molecular biology ( photo 1 ), to rapidly overcome them. (megazyme.com)
Given the important role of kinases in cellular regulation, radiometric assays can be applied to almost any biological research that investigates enzymatic activity within cellular processes. (kinasebiotech.com)
Development of enzymatic assays for the characterization of the acceptor specificity of thermostable ß - glucosidases. (lu.se)

Procedure: Assay takes 30 min. (wlsolutions.be)
This New Procedure follows a formal and transparent evaluation process. (who.int)

This detailed molecular understanding of cellular processes and pathways requires the ability to measure multiple analytes in parallel. (illinois.edu)
Nitric oxide (NO) is an important signaling molecule that regulates a diverse range of physiological and cellular processes in many tissues. (intechopen.com)
These new and provocative scientific perspectives on metabolism are being driven by enabling advances in metabolomics - the study of metabolites that are transformed during metabolism and visualised as functional maps of cellular processes. (ddw-online.com)
Virtually every area of life science research will benefit in some way from increased knowledge of the metabolic networks that underlie their relevant cellular processes. (ddw-online.com)
The majority of KEGG-annotated DEGs were involved in metabolism, genetic information processing, cellular processes, the organismal system, human diseases and environmental information processing. (biomedcentral.com)
These results facilitate the exploration of the molecular pathogenic mechanism of C. cassiicola in cucumbers and the understanding of molecular and cellular processes during spore germination. (biomedcentral.com)
Due to their critical role in many cellular processes, these enzymes are often under stringent control. (kinasebiotech.com)
Previous research showed that CLN5 participates in a variety of cellular processes. (frontiersin.org)

This has the potential of creating ultra-low-cost acoustofluidics devices, capable of carrying out complex microfluidic processing of samples for applications in flexible and wearable healthcare monitoring. (fluids.ac.uk)
Microfluidic devices for performing various assays were created with the tools in Lincoln Laboratory's Technology Office Innovation Laboratory. (mit.edu)

With current in vitro screening assays now representing a $1.4B market with a predicted 12% annual growth rate, we believe we possess a product capable of significantly impacting the chemical/drug screening market and, here in particular, advancing our understanding of cytotoxic chemical biotransformations as they pertain to public health and consumer safety. (inknowvation.com)
The drug discovery process requires that upwards of 10,000 molecules be screened for each new lead compound developed and relies upon an expensive and time consuming combination of in vitro cell culture-based and in vivo whole animal-based models to identify, validate, and ensure the safety of any resulting potential therapeutic agents. (inknowvation.com)
This process is frustratingly exacerbated by an unfortunate dichotomy whereby the inexpensive in vitro cell culture systems used for tier 1 screening contribute to failures due to their inabiliy to model the complexity and parallel systems interaction inherent in whole animal models (which are responsible for up to 92% of new compound failures at the clinical level) and because they are not capable of demonstrating species-specific effects. (inknowvation.com)

Equine DAT can induce anaphylactic reactions (a test for sensitivity to DAT should be conducted before each administration) ( 5 ). (cdc.gov)
Silver enhancement of the bound nanoparticles may be used to increase the sensitivity of the assay. (hindawi.com)

Up to 92% of failures for these new compounds at the clinical level are related to cytotoxicity, which often onl manifests during the costly and time consuming process of whole animal testing. (inknowvation.com)
These kits require luminescence plate readers, are frequently used for cell viability or cytotoxicity assays. (aatbio.com)

This course will cover the structure and function of different biological molecules, including proteins, carbohydrates, lipids and nucleic acids. (uaeu.ac.ae)
These systems have been used to direct the synthesis of artificial proteins having unnatural amino acids, in FDA-approved clinical assays for HIV, hepatitis B and hepatitis C that improves the medical care of over 400,000 patients annually, and to support the first artificial chemical system capable of Darwinian evolution. (thewestheimerinstitute.org)
Autophagy is a fundamental catabolic process coordinated by a network of autophagy-related (ATG) proteins. (babraham.ac.uk)
We have shown that this process is triggered by recruitment of ATG16L1 by the vacuolar-type H-translocating ATPase (V-ATPase) proton pump, suggesting a role for pH sensing in recruitment of Atg8-family proteins to single membranes. (babraham.ac.uk)
This assay format is versatile and compatible with virtually any type of kinase substrates, including peptides, proteins, small molecules, and lipids, as long as there is a suitable method to separate radiolabeled ATP and substrate. (kinasebiotech.com)
Overall, this study reveals the impact of cln5 -deficiency on gene expression in D. discoideum , provides insight on the genes and proteins that play a role in regulating Cln5-dependent processes, and sheds light on the molecular mechanisms underlying CLN5 disease. (frontiersin.org)
However, like most CLN proteins, the association of CLN5 with a defined biological pathway is still under investigation. (frontiersin.org)

Other articles in the CBRNE section (see CBRNE - Biological Warfare Agents and CBRNE - Evaluation of a Biological Warfare Victim) discuss the specific clinical management issues involved with treatment of patients exposed to potential bioterrorism pathogens and toxins. (medscape.com)

Therefore no filtration of reagent is needed prior to assays, as is often required with other commercial kits. (wlsolutions.be)
Fast and convenient: single reagent "mix-and-measure" assay allows quantitation of free phosphate within 30 minutes. (wlsolutions.be)
The Assay Creatineng Uk reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (biolisp.org)
Samples and standards are incubated for 30 minutes with a reaction reagent which changes color from yellow to bright orange upon reacting with creatinine, forming the creatinine-picrate complex. (biolisp.org)
During chemical experiment, test tube is most commonly seen experiment equipment, test tube, the conventional instrument of chemical laboratory Device, serves as the reaction vessel of a small amount of reagent, (should be preheated before heating, not so test tube is easily quick-fried in normal temperature or heating Split. (google.com)

The determination of the peroxides is performed by the reaction of a peroxidase with peroxides in the sample followed by the conversion of TMB to a colored product. (eaglebio.com)

This dissertation describes the fabrication, characterization, development, and application of silicon photonic microring resonator technology to multiplexed protein measurements in a variety of biological systems. (illinois.edu)
When the cell divides, the corrupt protein is contained in both daughter cells, where it seeds the process again. (sciencedaily.com)
According to Liebman, the finding that one prion can help start the chain reaction in another, totally different protein is important, because much more is known about how prions propagate than about how they first appear-which also bears on public health concerns. (sciencedaily.com)
b) Schematic drawing of a bioanalytical REA assay before (left) and after (right) binding of AuNP-analyte complexes to the capture protein layer. (hindawi.com)
Techniques used span from synthetic chemistry to protein expression and biochemical assays. (hofstra.edu)
Samples: Chemicals with carbonyl groups (e.g. ketones, aldehydes) or protein carbonyls in biological samples (e.g. oxidized BSA, etc). (gentaur-online.com)
Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals such as magnesium, iron and zinc. (gentaur-online.com)
This phosphorylation process regulates protein function and cell signaling, and if it goes wrong, it sometimes causes cancer. (acs.org)
A new study showed that use of the protein misfolding cyclic amplification (PMCA) assay was able to detect PrP Sc in 13 of 14 urine samples obtained from patients with variant CJD. (medscape.com)

Here, we report the synthesis, characterization, and use of fluorinated polysiloxane oils that have densities similar to the one of water at room temperature, and when mixed with non-ionic fluorinated surfactants, can produce droplets encapsulating biochemical reactions. (thewestheimerinstitute.org)

Detailed specimen collection and processing instructions are discussed in the NHANES Laboratory/Medical Technologists Procedures Manual (LPM). (cdc.gov)
Compatible with routine laboratory and HTS formats: assays can be performed in tubes, cuvettes or microplates, on spectrophotometers and plate readers. (wlsolutions.be)
New assays and biotechnologies, including physicochemical techniques, are also evaluated by the Expert Committee, as appropriate, through collaborative laboratory studies. (who.int)

A traditional assay would look for compounds that bind to an activated kinase. (acs.org)

cytokine quantitation is thus important in fundamental biological studies and diagnostics, and complex and overlapping cytokine roles make multiplexed measurements especially vital. (illinois.edu)

The assay can detect ~3 µM of ATP in a 100 µL reaction volume with minimal interference from ADP and AMP. (aatbio.com)
This assay is based on the reaction originally described by Griess to detect NO 2 - in a variety of biological fluids such as cell culture medium, plasma, serum, or urine. (ozbiosciences.com)
Among different kinase assays that have been developed to detect the activity of specific kinases in cells, radiometric assays have long been considered the "gold standard" assay format. (kinasebiotech.com)
Dr. Soto developed the PMCA assay and is the senior author of the study that used it to detect PrP Sc in urine samples taken from patients with CJD. (medscape.com)

In particular, the biological assay of potency recommended in 1991 had been shown not to correlate with the efficacy of the vaccine in infants, nor to provide a sensitive indicator of vaccine quality. (who.int)
Effective use of hierarchical lines of evidence relies on consideration of the rele- vance and potency of assays, organization of assays by mode of action, and better assay validation. (cdc.gov)

With a patented combination of monochromator and filter optics, Synergy Neo2 an advanced plate reader delivering flexibility and performance for any microplate assay. (agilent.com)

It detects choline, the ChE reaction product, though a multi-step process in which the oxidation of choline by choline oxidase results in the formation of H202, which is detected though a horseradish peroxidase-coupled reaction with 10-acetyl-3,7- dihydroxyphenoxazine (Amplex Red). (cdc.gov)
This assay detects DNA at varying concentrations, providing a qualitative result of either positive, negative, or inconclusive in the identification of Orthopoxvirus infections, other than smallpox (caused by variola virus). (cdc.gov)
This assay is the only format that directly detects the true product without any special substrate labeling or modification. (kinasebiotech.com)

Read the LABDOC file for detailed data processing and editing protocols. (cdc.gov)

Fields such as medical diagnostics and cellular biology would benefit greatly from a technology that enables rapid, quantitative and reproducible assays for many targets within a single sample. (illinois.edu)

These tests screen libraries of compounds to find any that affect the activity of biological targets, such as enzymes, that may be involved in a disease. (acs.org)

1994. Threshold limit values for chemical substances and physical agents and biological exposure indices (1994- 1995). (cdc.gov)
Various studies have been conducted on the biodegradability of cellulose acetate, but no review has been compiled which includes biological, chemical, and photo chemical degradation mechanisms. (springer.com)
It is referred as the \'molecular unit of currency\' of intracellular energy transfer to drive many processes and chemical synthesis in living cells. (aatbio.com)
Any field that relies on analyzing and identifying chemical and biological elements-for example, medicine, environmental protection, and agriculture-could benefit from the rapid, onsite assessments enabled by a lab-on-a-chip. (mit.edu)

For example, phosphatases, ATPases and several other enzymes catalyze reactions in which inorganic phosphate (Pi) is released from a substrate. (cmscorp.co.kr)

The objective of this study was to evaluate this fluorescent assay as a tool to measure ChE activities in biological specimens collected from humans and wildlife. (cdc.gov)

Terrorist activities, thefts from biological laboratories, or information received from police agencies may impart forewarning if provided in a timely, discrete manner to appropriate offices of the local healthcare network. (medscape.com)

AAT Bioquest offers a variety of bioluminescence assay kits to determine nanomolar (nM) range of ATP with recombinant firefly luciferase (Cat# 21610 & 21609). (aatbio.com)

Can be readily automated to process thousands of samples per day. (biolisp.org)
Add 50 µL of ATP working solution to each well of ATP standard, blank control, and test samples to make the total ATP assay volume of 100 µL/well. (aatbio.com)
Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. (gentaur-online.com)
Can be readily automated for processing thousands of samples per day. (gentaur-online.com)
Low interference in biological samples. (gentaur-online.com)
Samples: Biological, food and environment. (gentaur-online.com)
Using PMCA assay, PrP Sc was detectable in all but 1 of the urine samples obtained from the 14 patients with the variant form of CJD and in none of the 224 urine samples obtained from patients with sporadic CJD and other disorders, as well as healthy controls. (medscape.com)

Enzymes are catalysts of biological processes. (iucr.org)

The aim of this course is to apply the basic knowledge gained in Biochemistry (CHEM 361) course to specific metabolic reactions and certain physiologically important biomolecules. (uaeu.ac.ae)

FP Fluor™ superior fluorescent labelling dyes, optimised for developing fluorescence polarisation-based assays. (stratech.co.uk)

Cell Meter™ cellular functional assay kits, a complete set of tools for functional analysis of cellular events. (stratech.co.uk)
Other nitrogen assay kits also available. (megazyme.com)
The PhosphoWorks™ Colorimetric ATP Assay is complementary to our luciferase-based ATP assay kits. (aatbio.com)
A large collection of ready-to-use kinase activity assay kits are also available to help our customers save precious time. (kinasebiotech.com)

The most reliable fluids, immiscible with water and suitable for biochemical reactions, are fluorinated fluids. (thewestheimerinstitute.org)
Pointing to one device that resembles a CD disk imprinted with a pattern of thin lines (i.e., channels for the fluids), Walsh explains that a fluid, say a biological sample, is injected through a port in the disk. (mit.edu)

There is a rising interest in biotechnology for the compartmentalization of biochemical reactions in water droplets. (thewestheimerinstitute.org)
Span® 80, for example, a commonly used surfactant, has contaminants that interfere with various biochemical reactions. (thewestheimerinstitute.org)

It is of great interest to develop robust assays for monitoring their activities. (kinasebiotech.com)

Significant insight into their catalytic mechanisms has been obtained by relating site-directed mutagenesis studies to kinetic activity assays. (iucr.org)

By comparing a novel 1,2 dithiolane-based probe to the state-of-the-art probes, including commercial TrxR sensors, by screening a conclusive assay panel of cellular TrxR modulations, I clarified that 1,2 dithiolanes are not selective substrates for TrxR in biological settings (Nat Commun 2022). (uni-muenchen.de)

It is used to simulate the Monte Carlo method, ST naturally provides ensembles of kinetics of large signaling networks, where one cannot only solutions rather than single ones, subject to analysis by rely on biological intuition. (lu.se)

Radiometric filtration binding assays is universal and can be used for all kinds of kinases. (kinasebiotech.com)

These studies use a range of techniques, from computational modelling, to biological assays, to Nuclear Magnetic Resonance Spectroscopy (NMR). (hofstra.edu)

However, this motif was described elsewhere as thermodynamically instable and was due to widely used for dynamic covalent cascade reactions. (uni-muenchen.de)
To date, such biological markers have not been widely available nor have they been widely used when they are available. (cdc.gov)

Here, we review the diverse, and still expanding, collection of stimuli and processes now known to harness the noncanonical autophagy pathway, including engulfment processes, drug treatments, TRPML1 and STING signaling, viral infection, and other pathogenic factors. (babraham.ac.uk)

Add 100 µl reaction buffer mixture to appropriate wells. (eaglebio.com)

This structural information is then used to estimate the modifications of the reaction rate constants and the corresponding free-energy profiles of CA II catalysis. (iucr.org)
A structural-activity relationship for the estimation of rate constants for the gas-phase reactions of OH radicals with organic compounds. (cdc.gov)

The researchers also devised a screening test that can be used to pinpoint unidentified prions, making it the first such genetic assay for these mysterious biological agents. (sciencedaily.com)
They can be assembled into profiles and collated phenotypes that provide a functional readout for interpreting biological behaviour, in the context of both genetic and environmental factors. (ddw-online.com)

Also, process development work was done to adapt the preparation so that it was suitable for scale-up, and a subsequent large scale synthesis of the acetal was undertaken. (vuw.ac.nz)

Therefore, direct methods are necessary for investigating biological process and diseases related to NO in biological conditions. (intechopen.com)
By improving methods of collection and sample processing, the chances of obtaining better results and downstream analysis are increased. (hofstra.edu)
Methods to study reactions or processes taking place in an artificial environment outside the living organism. (bvsalud.org)
It provides an ensemble of high quality solutions, which are analyzed with clustering methods and display a hierarchical structure highlighting distinct parameter subspaces with biological interpretations. (lu.se)
In unsupervised methods, providing explanatory insights into addition, we predict the response of the system when varying the data and related biological interpretations. (lu.se)

Wang began working in the pharmaceutical industry in 2015, when he joined Janssen to develop biochemical and biophysical assays, which are fundamental tools in early-stage drug discovery. (acs.org)

Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
Advanced environmental controls, including CO 2 /O 2 control, incubation to 70 °C, and variable shaking support live cell assays. (agilent.com)
In the model organism Dictyostelium discoideum , loss of the CLN5 homolog, cln5 , impacts various cellular and developmental processes including cell proliferation, cytokinesis, aggregation, cell adhesion, and terminal differentiation. (frontiersin.org)

First, the integration of QIAseq FastSelect in the workflow allows for rapid and efficient removal of ribosomal RNA during the cDNA synthesis reaction. (qiagen.com)

To further display the wide array of sensing capabilities that could be used to carry out the mission, handheld lateral-flow immuno- assays were available in the DR SKO for presumptive screening of biological hazards, along with the Joint Handheld Biological Identifier. (army.mil)

The goal of the MLP is to screen compounds in the Small Molecule Repository in target-based and phenotypic assays to identify and subsequently optimize small molecule s as research probes. (nih.gov)
For the past few years, research on the pathogenic mechanism of C. cassiicola has mainly focused on biological characteristics, pathogenicity differentiation, cloning of virulence-associated genes, etc. (biomedcentral.com)
Creative BioMart offers high-quality services on kinase activity studies using radiometric assays to assist our customers in their kinase biology research and drug discovery. (kinasebiotech.com)
Introduction The research of Hatch (1) has greatly increased our understand- ing of the use and potential limitations of biological markers for adverse reproductive effects. (cdc.gov)

On the other hand we also assay the nutrients such as glucose, ammonia, phosphorus and protease during the fermentation. (ukessays.com)

Quebrachitol derivatives 305, 306 and 307 were prepared and subsequently tested against myoinositol (the optimal competitor) in biological uptake assays of the microorganisms, Candida albicans and Leishmania donovani. (vuw.ac.nz)

The WHO Expert Committee on Biological Standardization reviews developments in the field of biological substances, which include vaccines, blood products and biological therapeutics, and recommends procedures to assure their quality, safety and efficacy, including the establishment of international reference materials. (who.int)

1994. Biological monitoring of exposure to organophosphorus insecticides by assay of urinary alkylphosphates: influence of protective measures during manual operations with treated plants. (cdc.gov)
This study supports the utility of male germ cells as sensitive tools with which to assess the biological impacts of whole-body RF-EME exposure. (nature.com)
Exposure to a potential fetal health hazard can be estimated through ecological assessment (e.g., testing the community water supply), questionnaires (e.g., classifying residents accord- ing to whether they drink bottled or tap water), or biological markers (e.g., testing for exposures to chemicals or solvents found in tap water). (cdc.gov)
In this example, a biological exposure marker (blood lead-level) was available for classifying mothers according to their exposure to lead. (cdc.gov)

These new molecules could constitute an unlimited source of DAT, with a low risk for hypersensitivity reactions. (cdc.gov)
Categorize Biological Membranes And Various Mechanisms Of Transport Of Molecules Through Membranes. (uaeu.ac.ae)

Anatomy3

Organisms2

Chemicals and Drugs8

Analytical, Diagnostic and Therapeutic Techniques and Equipment11

Phenomena and Processes13

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Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture1

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Colorimetric assay4

Enzyme12

ELISA15

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Detection11

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High throughput3

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10001

QuantiChrom1

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Convenient assay2

Enzymatic4

Procedure2

Cellular processes8

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Proteins7

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Characterization1

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