Processes tumor cells cultured. Medical search. Frequent questions

CSCs are enriched near macrophages, particularly near macrophage-containing intravasation sites called Tumor Microenvironment of Metastasis (TMEM) doorways. (nature.com)
Herein, we report near-infrared light and tumor microenvironment (TME), dual responsive as well as size-switchable nanocapsules. (nature.com)
Thus, addressing the presence of immunosuppressive myeloid cell populations in the periphery and tumor microenvironment of patients with cancer is likely to be required for effective NK cell-based immunotherapy. (elifesciences.org)
However, modeling the complexity of cancer utilizing these cell lines on standard plastic substrata, does not accurately represent the tumor microenvironment. (edu.au)
For their studies, Altieri's team cultured breast cancer cells in a low-oxygen setting to mimic a condition known as hypoxia, which is a hallmark of the microenvironment surrounding most solid tumors, and studied the EV s released by these cells. (wistar.org)
Small Extracellular Vesicle Regulation of Mitochondrial Dynamics Reprograms a Hypoxic Tumor Microenvironment, Developmental Cell (2020). (wistar.org)
Further, their RNA-seq analysis found that diversification of methylation and transcriptome state occurred within each cancer and this was apparently heritable, stable, and independent of the tumor microenvironment, as it persisted after the organoids were cultured in vitro . (genomeweb.com)
We are interested in whether age-dependent accumulation of senescent cells leads to alterations in tissue microenvironment that is favorable for oncogenesis. (umassmed.edu)

Cancer stem cells (CSCs) play an important role during metastasis, but the dynamic behavior and induction mechanisms of CSCs are not well understood. (nature.com)
Infiltration versus metastasis is one of the most characteristic biological properties of tumors [ 5 ]. (hindawi.com)
Generally, it is believed that there are four main pathways between tumor invasion and metastasis, including local invasion, blood, lymphatic, and seeding metastasis [ 6 ]. (hindawi.com)
In addition to these four pathways, tumors can undergo infiltration around nerve fibers and metastasis along nerves, that is, perineural invasion (PNI), which refers to the phenomenon of perineural invasion by tumor cells filling the perineurial space, wrapping around nerves in a continuous concentric sheath-like pattern, infiltration, and metastasis of extension along nerves around nerve fibers or into perineurium within the perineurium [ 7 ]. (hindawi.com)
Functional analysis determined that DOCK10 , ITGA11 , DAB2 , PDFGRA , VASN , PPAP2B , and LPAR1 are highly expressed in trailblazer cells and required to initiate collective invasion, with DOCK10 essential for metastasis. (jci.org)
Together, our results indicate that spontaneous conversion of the epigenetic state in a subpopulation of cells can promote a transition from in situ to invasive growth through induction of a cooperative form of collective invasion and suggest that therapeutic inhibition of trailblazer cell invasion may help prevent metastasis. (jci.org)
Bone metastasis occurs through a complex cascade of events that ultimately results in circulating tumor cells (CTCs) from the blood extravasating and invading the bone marrow - these cells within the bone marrow are referred to as disseminated tumor cells (DTCs) [ 4 ]. (oncotarget.com)
Sign up for free Altmetric alerts about this article - https://oncotarget.altmetric.com/details/email_updates?id=10.18632%2Foncotarget.27510 Press release - https://www.oncotarget.com/news/pr/cancer-associated-fibroblasts-confer-shear-resistance-to-circulating-tumor-cell/ Keywords - metastasis, cancer associated fibroblasts, circulating tumor cells, cytoprotection, collective migration About Oncotarget Oncotarget is a peer-reviewed, open access biomedical journal covering research on all aspects of oncology. (labtube.tv)
The cancer stem cell theory holds that tumors also contain stem-like cells that drive tumor growth and metastasis formation. (medscape.com)
The CSC theory is attractive because it offers an explanation for tumor heterogeneity, for the inefficiency of metastasis formation and for tumor regrowth after an apparently complete remission. (medscape.com)
Patients diagnosed with sarcomatoid clear cell renal cell carcinoma face a considerably worse prognosis due to an increased propensity for metastasis. (lu.se)

Here, we describe the pre-clinical in vitro and in vivo study of irradiated haNK cells engineered to express a second-generation chimeric antigen receptor (CAR) targeting programmed death-ligand 1 (PD-L1). (elifesciences.org)
To reveal that the NGF-TrkA signaling pathway was closely associated with PC PNI, in vitro neuroinvasion model was established by using MiaPaCa-2 cells via coculturing DRG cells in Matrigel. (hindawi.com)
In vitro , the sprouting and migratory properties of primary endothelial cell cultures were reduced by inhibiting EZH2 through up-regulation of miR-101, siRNA-mediated knockdown of EZH2, or treatment with 3-Deazaneplanocin-A (DZNep), a small molecule inhibitor of EZH2 methyltransferase activity. (plos.org)
As linear correlations exist between expression levels of angiogenic factors under normoxic and hypoxic conditions in vitro , we propose that explanted primary cells may be used to probe the in vivo hypoxic environment. (biomedcentral.com)
In vitro studies have shown that stromal cells cultured in hypoxic growth conditions secrete higher levels of critical angiogenesis-inducing factors than cells cultured in normoxic conditions [ 3 ]. (biomedcentral.com)
The researchers discovered that the protocells were able to produce energy-rich molecules (ATP) via glycolysis and synthesize RNA and proteins by in vitro gene expression, indicating that the inherited bacterial components remained active in the synthetic cells. (nanowerk.com)
According to the researchers, this approach, which uses the simultaneous tuning of surface hydrophobicity and topography, could provide a platform for tumor detection in vitro. (chemistryviews.org)
There is an unmet clinical need to extract living circulating tumor cells (CTCs) for functional studies and in vitro expansion to enable drug testing and predict responses to therapy in metastatic cancer. (lu.se)
The novel A2 method provides extensive elimination of WBCs combined with the gentle recovery of viable cancer cells suitable for downstream functional analyses and in vitro culture. (lu.se)
Transforming growth factor β1 exposure of in vitro cultured primary clear cell renal cell carcinoma cells resulted in cells adopting a mesenchymal morphology similar to sarcomatoid clear cell renal cell carcinoma. (lu.se)
therefore, WS serves as a model for studying the aging process in vivo and in vitro. (medscape.com)

It was during this time that I developed a passion for studying cancer stem cell biology and the molecular signaling that promotes glioblastoma (GBM) tumor progression. (labmanager.com)
Professor Silvia Marino and her team are studying glioblastoma multiforme (GBM) , the most aggressive and common primary high-grade brain tumour in adults, as well as some rarer primarily childhood tumours such as choroid plexus. (braintumourresearch.org)
Moreover, we found that glioblastoma cells, notorious for their VEGF production, elicited a similar response when co-cultured with endothelial cells [11] . (plos.org)
In 2014, he and collaborators demonstrated that gold nanoparticles could be used to 'smuggle' chemotherapy drugs into cancer cells in glioblastoma multiforme, the most common and aggressive type of brain cancer in adults, which is notoriously difficult to treat. (cam.ac.uk)
A coating on the particles made them attracted to tumour cells from glioblastoma patients, so that the nanostructures bound and were absorbed into the cancer cells. (cam.ac.uk)
Background: The technology related to the establishment of primary tumor cell cultures of solid tumors, including glioblastoma, which is increasingly important for oncology research and practice. (viromer-transfection.com)

MiaPaCa-2 and CFPAC-1 cells were treated with 100 ng/ml of NGF or the NGF inhibitor Tanezumab for 24 h, CCK-8 and Transwell assays were employed to test cell proliferation, invasion, and migration, respectively. (hindawi.com)
The activation of the PDGF receptor tyrosine kinase triggers the proliferation of DFSP tumor cells. (medscape.com)
VEGF, produced in large amounts by cancer cells during tumor growth, interacts with its receptors VEGFR1 and VEGFR2 thereby causing endothelial cell survival, proliferation, and sprouting [7] . (plos.org)
Although these tumors are considered to be non-malignant, they have a high rate of proliferation and can reach large sizes before being diagnosed. (frontiersin.org)
Amino acids are essential for cellular homeostasis, growth and proliferation via their contribution to a diverse range of cellular processes. (spandidos-publications.com)
We demonstrated that while intestinal epithelial cell (IEC) proliferation and death are unaltered in mice with ubiquitous suppression of NF-κB activity, goblet cell numbers increase at the expense of Paneth cells. (hu-berlin.de)
Furthermore, aberrant oncogenic activation, DNA damage or oxidative stress activates senescence, providing a failsafe mechanism that prevents the proliferation of cells at risk of neoplastic transformation. (umassmed.edu)
A) Lymphomas found in Smurf2-deficient mice have the characteristics of diffuse large B-cell lymphoma (DLBCL) and enhanced cell proliferation. (umassmed.edu)
Here we determined that a reactive state of CAFs proved to be important for supporting tumor cell survival and proliferation. (labtube.tv)
He proposed that the study of rapidly multiplying bacteria cultures might therefore be useful in cell genetics research and even illuminate the cell proliferation that characterized tumor growth. (nih.gov)

Failed T cell-based immunotherapies in the presence of genomic alterations in antigen presentations pathways may be overcome by NK cell-based immunotherapy. (elifesciences.org)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Western blot analysis was performed to study Boswellia sacra essential oil-regulated proteins involved in apoptosis, signaling pathways, and cell cycle regulation. (biomedcentral.com)
Consistently, the essential oil represses signaling pathways and cell cycle regulators that have been proposed as therapeutic targets for breast cancer. (biomedcentral.com)
In addition to modulation of mitochondrial behavior, the research team discovered that sEV released by hypoxic breast cancer cells induced major changes in gene expression in the normal recipient cells, with activation of multiple pathways of cell motility, cytoskeletal organization and cell-to-cell contact. (wistar.org)
Their expression in tumour cells is modulated by a complex interplay of genomic, transcriptomic and post translational factors involving multiple intracellular antigen processing pathways. (portlandpress.com)
Transcriptomics analyses showed pathways related to TCR signaling, cytotoxicity and oxidative phosphorylation were significantly enriched in Tet lo found in both regressing and progressing tumors compared with Tet hi , whereas genes related to DNA damage, apoptosis and autophagy were downregulated. (bmj.com)

In mammary gland development, Notch-dependent heterotypic signaling between resident tissue macrophages and mammary stem cells supports survival and function of the normal mammary stem cell 20 . (nature.com)
Tumor cells can engage in a process called collective invasion, in which cohesive groups of cells invade through interstitial tissue. (jci.org)
Tumor tissue from a rare non-infantile 8-year-old female DIG patient was dissociated and digested using collagenase to establish live cultures. (frontiersin.org)
Cells cultured as 3D were embedded as tissue blocks. (frontiersin.org)
Using immunohistochemistry, the 3D cultures were shown to express markers as in the original DIG tumor tissue indicating that the spheroid cultures were able to maintain the heterogeneity found in the original tumor. (frontiersin.org)
The tumor is heterogeneous with meningeal tumor cells, mainly a mixture of fibroblast like cells, and neuroepithelial cells in a background of connective tissue. (frontiersin.org)
They can be injected in the same way as a normal drug, and are carried through the bloodstream to the target organ, tissue or cell. (cam.ac.uk)
As a tumor grows, the existing blood supply becomes inefficient at supporting the tissue, and areas of the tumor become hypoxic. (biomedcentral.com)
This protocol is amenable not only for detection and quantification purposes but also to study the expression of markers of numerous biological processes or tissue-specificity. (oncotarget.com)
These messages are packaged into particles called extracellular vesicles ( EV s) and reprogram mitochondrial shape and position within the recipient normal cells to ultimately promote deregulated tissue morphogenesis. (wistar.org)
Biointerfaces, the surfaces between living cells and other materials , are important for applications in, e.g., biomedicine and tissue engineering. (chemistryviews.org)
Consistent with this notion, we have found that Smurf2-deficient mouse embryonic fibroblasts exhibit delayed senescence entry and enhanced potential to become immortalized in culture, while Smurf2-deficient mice show increased susceptibility to various types of cancer, including B-cell lymphoma, hepatocellular carcinoma, adenocarcinoma in small intestine and soft tissue sarcoma. (umassmed.edu)
However, the progenitor cells consist of T cells isolated from resected tumor tissue. (msdmanuals.com)
Normal multipotent tissue stem cells (SCs) are the driving force behind tissue turnover and repair. (medscape.com)
Normal proliferative tissues contain multipotent stem cells (SCs) that drive tissue turnover. (medscape.com)
By contrast, transit amplifying cells and differentiated cells are incapable of self-renewal and tissue regeneration. (medscape.com)
6] Since then, numerous histochemical, immunohistochemical, electron microscopic, and tissue culture studies have supported the neural crest origin and confirmed the preferred term of melanotic neuroectodermal tumor of infancy. (medscape.com)
Oral microorganisms may also enter the deeper tissue after trauma or surgery, which contributes to the disease process, particularly when they cause BE. (medscape.com)

We postulated that the expression levels of angiogenesis-related proteins from various primary tumor cultures would be greater under hypoxic conditions than under normoxia. (biomedcentral.com)
Our recent studies as well as others indicate that senescent cells change their secretory patterns of cytokines, chemokines, growth factors, extracellular matrix proteins and proteases. (umassmed.edu)
This meant that the gene-enzyme relationship was more complex than the one gene-one enzyme theory recently proposed by Edward Tatum and George Beadle: genes controlled not the direct synthesis of enzymes and other cell proteins, but only the potentiality of such synthesis. (nih.gov)
Chimeric antigen receptors (CAR) recognize specific proteins on the surface of tumor cells. (msdmanuals.com)
In contrast to TCR T cells, CAR T cells recognize only relatively large proteins on the surface of tumor cells. (msdmanuals.com)
Cancer stem cell-associated proteins revealed by proteomics. (medscape.com)

GBM stem-like cells (GSCs) present in tumors are felt to be highly tumorigenic and responsible for tumor recurrence. (oncotarget.com)
DFSP is a locally aggressive tumor with a high recurrence rate. (medscape.com)
In cases where multiple other mutations, in genes such as TP53, ATRX, and others, recurrence of the tumor is a possibility. (frontiersin.org)
DTCs occupy the hematopoietic stem cell niche within the bone marrow [ 5 ], often remaining dormant for years before becoming reactivated, leading to cancer recurrence [ 6 ]. (oncotarget.com)
A better understanding of this process may give us important clues about how tumors hijack nearby normal cells to promote disease recurrence. (wistar.org)

Macrophages are emerging as major cellular factors in the tumor ecosystem that can influence the stem phenotype and cancer progression. (nature.com)
Notch signaling has been implicated in many microenvironmental processes associated with cancer progression such as vascular remodeling, immunosuppression, epithelial to mesenchymal transition (EMT), as well as maintenance of the CSC pool 21 . (nature.com)
GBM stem-like cells (GSCs) represent a subpopulation of the tumor which is responsible for tumor initiation, progression, and re-growth after chemoradiation [ 5 , 6 ], as well as supporting tumor vessel growth and function [ 7 ]. (oncotarget.com)
Boswellia sacra essential oil hydrodistilled at 100 o C was more potent than the essential oil prepared at 78 o C in inducing cancer cell death, preventing the cellular network formation (MDA-MB-231) cells on Matrigel, causing the breakdown of multicellular tumor spheroids (T47D cells), and regulating molecules involved in apoptosis, signal transduction, and cell cycle progression. (biomedcentral.com)
The resulting aneuploidy is a key event in the progression of cancer and is correlated with tumor stage. (cdc.gov)
It is well known that cancer cells 'talk' to their neighboring normal cells all the time and this is important to promote cancer progression," said study lead author Dario C. Altieri, M.D. , Wistar president and CEO , director of the Institute's Cancer Center and the Robert & Penny Fox Distinguished Professor. (wistar.org)
Our findings indicate that breast cancer cells may use sEVs to enable both local and distant disease progression," said Irene Bertolini, Ph.D., first author of the study and a postdoctoral fellow in the Altieri lab. (wistar.org)
Dr. Nerymar Ortiz-Otero from the Meinig School of Biomedical Engineering, Cornell University, discusses a research paper she co-authored that was published by Oncotarget in Volume 11, Issue 12, entitled, "Cancer associated fibroblasts confer shear resistance to circulating tumor cells during prostate cancer metastatic progression. (labtube.tv)

Here, we demonstrate that NK cells (haNKs) engineered to express a PD-L1 chimeric antigen receptor (CAR) haNKs killed a panel of human and murine head and neck cancer cells at low effector-to-target ratios in a PD-L1-dependent fashion. (elifesciences.org)

Q: Can you discuss some key technical and experimental challenges in culturing and using 3D cellular models? (labmanager.com)
Our aim was to establish a live cell culture to enable the understanding of the cellular processes driving the non-malignant growth of these tumors. (frontiersin.org)
Anti-invasive and anti-multicellular tumor properties were evaluated by cellular network and spheroid formation models, respectively. (biomedcentral.com)
Suppression of cellular network formation and disruption of spheroid development of breast cancer cells by Boswellia sacra essential oil suggest that the essential oil may be effective for advanced breast cancer. (biomedcentral.com)
however, it is evident that further research is required for the development of more complex models that incorporate the majority of the cellular and physical properties of a tumor. (edu.au)
The factors and cellular processes required for the induction of dormancy and subsequent tumor cell reactivation remain largely undefined [ 6 ]. (oncotarget.com)
Culturing cells in three dimensions allows more physiologically relevant observations to be made regarding cellular interactions and gene expression. (selectscience.net)
By definition, mutations that cause CIN in cancer cells produce "sub-lethal" deficiencies in an essential cellular process (chromosome maintenance) and therefore may represent genetic vulnerabilities in tumor cells that could be exploited for therapeutic benefit in the treatment of cancer. (ubc.ca)
In passive cellular immunotherapy, specific effector cells are directly infused and are not induced within the patient. (msdmanuals.com)
Passive cellular immunotherapy references A number of immunologic interventions, both passive and active, can be directed against tumor cells. (msdmanuals.com)
In passive cellular immunotherapy, specific effector cells. (msdmanuals.com)
Tonnessen-Murray and colleagues found that senescent cancer cells activate a group of genes that are normally active in white blood cells that engulf invading microbes or cellular debris. (medicalxpress.com)
After "eating" their neighbors, senescent cancer cells digested them by delivering them to lysosomes, acidic cellular structures that are also highly active in senescent cells. (medicalxpress.com)
According to the cancer stem cell (CSC) concept, the cellular hierarchy in normal tissues is preserved in a number of hematological and solid malignancies. (medscape.com)

C ) Representative H&E-stained primary breast tumors. (jci.org)

In vivo experiments demonstrate that these nanocapsules can target to tumor sites through fluorescence/magnetic resonance imaging and offer remarkable therapeutic results. (nature.com)
As the former director of the Starr Foundation Cerebral Organoid Translational Core at Weill Cornell Medicine, I established a novel, ex vivo 3D system to study the interactions and molecular crosstalk between brain tumor cells and a miniature model of the human brain. (labmanager.com)
Subsequently, we used a model of neuroinvasion in nude mice to assess the effect of NGF in vivo on tumor nerve invasion as well as on nociceptive transmission. (hindawi.com)
In vivo tumorigenesis experiments, Tanezumab markedly alleviated nerve invasion of PC cells as well as relieved nociceptive conduction in animal models. (hindawi.com)
Researchers of the study published in the journal Science Translational Medicine stated that they "demonstrate mutant IDH1 -dependent PARP inhibitor sensitivity in a range of clinically relevant models, including primary patient-derived glioma cells in culture and genetically matched tumor xenografts in vivo . (darkdaily.com)
Establishing 3D organoid culture from the small intestine and using different mouse lines, we prove in the second part of the thesis that NF-kB plays an important function in cell differentiation and stem cell maintenance in vivo and in ex-vivo. (hu-berlin.de)
3D models provide a more accurate representation of cells in vivo and are more stable than 2D cultures, making them very useful for long-term studies. (selectscience.net)
1990. Cell replication and unscheduled DNA synthesis (UDS) activity of low molecular weight chlorinated paraffins in the rat liver in vivo. (cdc.gov)
[ 4 ] In vivo aging is linked with accelerated aging of fibroblasts in culture, possibly due to the genomic instability, a hallmark of WS. (medscape.com)

Tumor-infiltrating lymphocytes (TILs) may have greater tumoricidal activity than LAK cells. (msdmanuals.com)
In the Multicenter AIDS Cohort Study (MACS), an ongoing prospective epidemiologic investigation of the transmission and natural history of HIV infection among homosexual men (3), there was a strong association (p less than 0.001) between the baseline numbers of T-helper lymphocytes (CD4+ cells) and the incidence of PCP (Table 1). (cdc.gov)
Similar results were seen when MACS data were analyzed by fraction of CD4+ cells expressed as a percentage of total lymphocytes rather than by absolute number of such cells. (cdc.gov)

Malignant gliomas remain aggressive and lethal primary brain tumors in adults. (oncotarget.com)
We are interested in how gene expression is regulated in the brain and how this process influence neurodegenerative diseases, psychiatric disorders and brain tumors. (lu.se)

TumorGenesis is developing a new approach to growing tumor models in the laboratory that is faster, less costly and more closely mimics the characteristics of the patient's tumor, than the traditional PDx animal models that are currently on the market. (globenewswire.com)
The 48HD ligand discovery technology is vital to the first step in this process as it screens the patient's tumor cells against large peptide libraries to identify the specific peptide ligands that bind to those cells. (globenewswire.com)
This is key to reassembling the tumor on an artificial 'scaffold', or 3D biomimetic support, so it grows in a way that closely mimics the patient's body. (globenewswire.com)
A new study has revealed that a new lab-on-a-chip process can test whether a patient's tumour cells will react to a given drug. (medindia.net)
The chips sit in small wells, known as microtiter plates, and are covered with a patient's tumor cells. (medindia.net)
Scientists at the Swiss Institute for Experimental Cancer Research have developed a modification that allows dendritic cells to acquire antigens from a patient's tumor. (issels.com)
In a process called extracorporeal photophoresis, a patient's blood passes through an ultraviolet light chamber, which provides an immune boosting effect. (issels.com)
Lymphokine-activated killer (LAK) cells are produced from the patient's T cells which are extracted from the tumor and grown in a cell culture system with the lymphokine interleukin-2 (IL-2). (msdmanuals.com)
The LAK cells produced are then returned to the patient's bloodstream. (msdmanuals.com)

Amanda Linkous , PhD, research associate professor, Department of Biochemistry and scientific center manager, NCI Center for Systems Biology of Small Cell Lung Cancer at Vanderbilt University , talks to contributing editor Tanuja Koppal, PhD, about new scientific and technological advancements in 3D cell culture. (labmanager.com)
The findings described here improve our understanding of NF-κB functions in stem cell biology which, when deregulated, also have an impact on intestinal inflammation and tumorigenesis. (hu-berlin.de)
The study, which will be published September 17 in the Journal of Cell Biology , suggests that this act of cannibalism provides these cancer cells with the energy they need to stay alive and initiate tumor relapse after the course of treatment is completed. (medicalxpress.com)

Cytokeratin-negative CTCs are characterised by the lack of EpCAM or cytokeratins, which may indicate an undifferentiated phenotype (circulating cancer stem cells) or the acquisition of a mesenchymal phenotype (known as epithelial-mesenchymal transition or EMT). (wikipedia.org)
CSCs display the slow-migratory, invadopod-rich phenotype that is the hallmark of disseminating tumor cells. (nature.com)
Furthermore, macrophages were shown to contribute to a niche that can support and maintain a breast CSC (BCSC) phenotype through heterotypic CD90/EphA4 signaling between macrophage and tumor cell 14 . (nature.com)
Canonical mesenchymal markers were not sufficient to distinguish trailblazer cells from non-trailblazer cells, suggesting that defining the molecular underpinnings of the trailblazer phenotype could reveal collective invasion-specific mechanisms. (jci.org)
Each tumor type reflected the initial phenotype from the parent culture cells. (suamaytinhhaiphong.com)
This reactive CAF phenotype emerges from normal fibroblasts (NF), which take on the CAF phenotype when co-cultured with tumor cells. (labtube.tv)

Small CTCs have been implicated in progressive disease and differentiation into small cell carcinomas, which often require a different therapeutic course. (wikipedia.org)
Inhibiting this process may provide new therapeutic opportunities, because we know that it is the breast cancer patients with tumors that undergo TP53 -mediated senescence in response to chemotherapy that have poor response and poor survival rates," Jackson says. (medicalxpress.com)

Otherwise, their morphology, gene expression and genomics are similar to those of other cancer cells. (wikipedia.org)
Measuring the ratio of traditional CTC to apoptotic CTCs-from baseline to therapy-provides clues to treatment efficacy in targeting and killing cancer cells. (wikipedia.org)
Mechanistically, stemness is induced in non-stem cancer cells upon their direct contact with macrophages via Notch-Jagged signaling. (nature.com)
In breast cancers from patients, the density of TMEM doorways correlates with the proportion of cancer cells expressing stem cell markers, indicating that in human breast cancer TMEM doorways are not only cancer cell intravasation portals but also CSC programming sites. (nature.com)
Once identified by 48HD's technology, SyntArray's targeted peptide cell capture technology screens these peptides to determine the best combination that will capture the cancer cells and allow them to attach and grow on the 3D biomimetic support. (globenewswire.com)
Spheroids, however, are cell aggregates typically composed of cancer cells cultured under scaffold-free, non-adherent conditions. (labmanager.com)
Multifunctional magnetic iron-oxide nanoparticles (IONPs) can be directly imaged by magnetic resonance imaging (MRI) and designed to therapeutically target cancer cells. (oncotarget.com)
They then introduced cytotoxic T cells in proximity to the cancer cells and monitored their behavior by microscopy. (pasteur.fr)
As a result of this high drug concentration, healthy cells may be killed as well as cancer cells, leaving many patients weak, nauseated and vulnerable to infection. (cam.ac.uk)
The objective of this study was to optimize conditions for preparing Boswellea sacra essential oil with the highest biological activity in inducing tumor cell-specific cytotoxicity and suppressing aggressive tumor phenotypes in human breast cancer cells. (biomedcentral.com)
Similar to our previous observations in human bladder cancer cells, Boswellia sacra essential oil induces breast cancer cell-specific cytotoxicity. (biomedcentral.com)
In addition, a range of molecular mechanisms deconstructed by studying cancer cells in 3D models suggest that tumor cells cultured in two-dimensional monolayer conditions do not respond to cancer therapeutics/compounds in a similar manner. (edu.au)
It is the result of the dissemination of cancer cells to the bone marrow. (oncotarget.com)
Although the LAT family plays important roles in the development and function of normal tissues, they are frequently increased in cancer cells ( 3 ). (spandidos-publications.com)
PHILADELPHIA - (Aug. 10, 2020) - According to a study by The Wistar Institute, breast cancer cells starved for oxygen send out messages that induce oncogenic changes in surrounding normal epithelial cells. (wistar.org)
To dissect the effects of sEVs produced by cancer cells on normal neighboring cells, researchers incubated normal breast epithelial cells with sEVs released by cells maintained in hypoxia. (wistar.org)
Shutao Wang, Chinese Academy of Sciences, Beijing, and colleagues have developed hydrogel coatings that can capture and release cancer cells. (chemistryviews.org)
This protein was linked to biotinylated epithelial-cell adhesion-molecule antibodies (anti-EpCAM), which can bind human breast cancer cells. (chemistryviews.org)
The hydrogel can capture cancer cells at 37 °C, since its surface "collapses" and develops nanowrinkles, which improve cell adhesion by providing a larger contact area (pictured). (chemistryviews.org)
Smart Thin Hydrogel Coatings Harnessing Hydrophobicity and Topography to Capture and Release Cancer Cells , Luying Wang, Hongliang Liu, Feilong Zhang, Guannan Li, Shutao Wang, Small 2016 . (chemistryviews.org)
Dendritic cells can distinguish normal dying cells from cancer cells so the immune system refrains from attacking them. (issels.com)
Colorectal cancer cells showed extensive mutational diversification and carried several times more somatic mutations than normal colorectal cells. (genomeweb.com)
Further, when the researchers exposed the tumors to anti-cancer drugs, they observed that even closely related cells in the same tumor had markedly different responses, indicating that colorectal cancer cells experience substantial increases in the rates of their somatic mutations as compared to normal colorectal cells. (genomeweb.com)
Genetic diversification of each cancer is accompanied by pervasive, stable, and inherited differences in the biological states of individual cancer cells," the team added. (genomeweb.com)
Ongoing research investigates mechanisms invoked by cancer cells to abrogate MHC-I expression and attenuate anti-tumour CD8 + cytotoxic T cell response. (portlandpress.com)
Animal studies have shown that LAK cells are more effective against cancer cells than are the original endogenous T cells, presumably because of their greater number. (msdmanuals.com)
This image of a breast tumor formed in mice and treated with the chemotherapy drug doxorubicin shows that some cancer cells (red nuclei) have been engulfed by other cancer cells (green cell membrane). (medicalxpress.com)
Researchers from the Tulane University School of Medicine have discovered that some cancer cells survive chemotherapy by eating their neighboring tumor cells. (medicalxpress.com)
Chemotherapy drugs such as doxorubicin kill cancer cells by damaging their DNA, but cells that survive initial treatment can soon give rise to relapsed tumors. (medicalxpress.com)
Instead of dying in response to chemotherapy -induced DNA damage, these cancer cells generally just stop proliferating and enter a dormant but metabolically active state known as senescence. (medicalxpress.com)
In addition to surviving chemotherapy, these senescent cancer cells produce large amounts of inflammatory molecules and other factors that can promote the tumor's regrowth. (medicalxpress.com)
Understanding the properties of these senescent cancer cells that allow their survival after chemotherapy treatment is extremely important," says Crystal A. Tonnessen-Murray, a postdoctoral research fellow in James G. Jackson's laboratory at the Tulane University School of Medicine. (medicalxpress.com)
In the new study, Tonnessen-Murray and colleagues discovered that, after exposure to doxorubicin or other chemotherapy drugs , breast cancer cells that become senescent frequently engulf neighboring cancer cells. (medicalxpress.com)
The researchers observed this surprising behavior not only in cancer cells grown in the lab, but also in tumors growing in mice. (medicalxpress.com)
Lung and bone cancer cells are also capable of engulfing their neighbors after becoming senescent, the researchers discovered. (medicalxpress.com)
Breast cancer cells (red) being engulfed and delivered to the lysosomes (green) of a doxorubicin-treated senescent breast cancer cell. (medicalxpress.com)
Importantly, the researchers determined that this process helps senescent cancer cells stay alive. (medicalxpress.com)
Senescent cancer cells that engulfed a neighboring cell survived in culture for longer than senescent cancer cells that didn't. (medicalxpress.com)
The researchers suspect that consuming their neighbors may provide senescent cancer cells with the energy and materials they need to survive and produce the factors that drive tumor relapse. (medicalxpress.com)
Here, we present a novel two-step acoustophoresis (A2) method for isolation of unfixed, viable cancer cells from red blood cell (RBC) lysed whole blood. (lu.se)
This acoustofluidic step enriches viable cancer cells in a central outlet, but a significant number of white blood cells (WBCs) remain in the central outlet fraction due to overlapping acoustophysical properties of these viable cells. (lu.se)
Using samples with 10 000 DU145 cells, we obtained 459 ± 188-fold depletion of WBC and 42% recovery of viable cancer cells. (lu.se)

On the contrary, small size NPs with a diameter of 4-20 nm easily penetrate into deep tumor tissues, but they are more prone to rapid clearance and insufficient drug retention. (nature.com)
Western blotting was employed to determine NGF level in PC and paracarcinoma tissues and in PC cell lines as well as pancreatic ductal epithelial cells. (hindawi.com)
NGF level was preeminently higher in PC tissues and cell lines than in paracarcinoma tissues and normal pancreatic epithelial cell lines. (hindawi.com)
Tumors, like the tissues from which they originate, contain a unique SC population that is capable of self-renewal and of sustaining tumor growth indefinitely. (medscape.com)

However, one study has reported that contrary to existing consensus, at least a discrete population of these clusters are non-malignant, and derive instead from the tumor endothelium. (wikipedia.org)
Natural killer (NK) cell-based immunotherapy may overcome genetic mechanisms of resistance to T cell-based immunotherapy through antigen- and MHC-independent recognition of malignant cells. (elifesciences.org)
Death usually occurs when patients are aged 30-50 years because of atherosclerosis or malignant tumors. (medscape.com)

The innovative 48HD technology allows us to capture all the heterogeneity of the tumor, including both the cancerous and non-cancerous cells. (globenewswire.com)
The CSC is defined as a cancer cell with self-renewing capacity that can regenerate the original tumor in all its differentiated heterogeneity. (medscape.com)

Bednar tumor, a pigmented variant of dermatofibrosarcoma protuberans, contains melanin-rich dendritic cells scattered among neoplastic spindle-shaped cells. (medscape.com)
Dendritic cell vaccines are normally created by force-feeding dendritic cells with tumor antigens. (issels.com)
The EVIR has been optimized to enhance dendritic cells and their ability to selectively uptake cancer cell-derived EVs. (issels.com)
The EVIR helps dendritic cells target the exosomes more precisely and present them to killer T-cells for a more efficient immune response. (issels.com)
De Palma explained the phenomenon of cross-dressing, in which dendritic cells display the acquired antigens directly on their surface. (issels.com)
Dendritic cells are part of the immune system, serving as advance sentries that recognize the presence of foreign antigens. (issels.com)
Once the dendritic cells process the antigens, they are presented to T cells, another part of the immune system. (issels.com)
Individually, dendritic cells are extremely potent, but the problem is that they're not usually present in large enough quantities. (issels.com)
White blood cells are harvested and cultured into active dendritic cells, then re-injected into the body to trigger an immune response. (issels.com)
Immunobiology of dendritic cells. (scriptiebank.be)

Researchers from the Princess Margaret Cancer Centre , which is associated with the University of Toronto Faculty of Medicine , have discovered that some tumors in prostate cancer have a genetic fingerprint that may indicate whether or not the disease will become more aggressive and less responsive to treatment. (darkdaily.com)
In another study, researchers looked at 15 patients with BRCA2-inheritied prostate cancer and compared the genomic sequences of those tumors to a large group of sequences from tumors in less-aggressive cancer cases. (darkdaily.com)

Dr. Carl Schwartz, Chief Executive Officer of Precision Therapeutics, commented, "TumorGenesis is developing a better way to grow tumors outside the human body so they mimic the environment inside the patients' body as closely as possible. (globenewswire.com)
The TumorGenesis PDx model will initially be developed for three orphan cancers, Multiple Myeloma, Triple-Negative Breast cancer (TNBC) and Ovarian cancers, all of which are areas that have a high unmet need for new and effective treatments that are tailored to patients' unique tumor profiles. (globenewswire.com)
PD-L1 CAR haNKs reduced levels of macrophages and other myeloid cells endogenously expressing high PD-L1 in peripheral blood from patients with head and neck cancer. (elifesciences.org)
This microfluidic system developed with the support of the Institut Pasteur Biomaterials and Microfluidics Platform could one day be used to perform personalized diagnostics by directly incorporating patients' cells to help predict their immune response. (pasteur.fr)
In 10-20% of patients with this tumor, trauma at the site seems to be incriminated. (medscape.com)
Annual incidence of Bednar tumor among blacks is 7.5 times higher than that of white patients. (medscape.com)
Due to the difficulty in sampling and detection, few techniques exist to efficiently and consistently detect and quantify disseminated tumor cells (DTCs) in the bone marrow of cancer patients. (oncotarget.com)
The dendritic cell vaccine is one of the most potent weapons in our cancer-fighting arsenal, succeeding in many late-stage patients for whom other treatments had failed. (issels.com)
Dendritic cell vaccines are only one of the innovative treatments we use for patients with all forms of cancer. (issels.com)
It outlines current approaches employed at the preclinical and clinical trial interface towards reversing these processes in order to improve response to immunotherapy and survival outcomes for patients with cancer. (portlandpress.com)
1970. Tumors of the urinary bladder: An analysis of the occupations of 1030 patients in Leeds, England. (cdc.gov)
Several patients with melanotic neuroectodermal tumor of infancy (MNTI) have demonstrated a high urinary excretion of VMA. (medscape.com)
Most patients, by some estimates more than 90%, present with the tumor in the first year of life, usually from age 1-6 months, with a peak between the second and sixth month of life. (medscape.com)

CSCs have an enhanced ability to self-renew, which makes them uniquely capable of initiating and sustaining primary and metastatic tumor growth 3 . (nature.com)
In addition, metastatic sites may re-seed primary tumors in a multi-directional fashion [ 8 ]. (oncotarget.com)
spread of metastatic cells [3]. (researchgate.net)

CTCs can extravasate and become seeds for the subsequent growth of additional tumors (metastases) in distant organs, a mechanism that is responsible for the vast majority of cancer-related deaths. (wikipedia.org)
On the other hand, CTCs are very rare, often present as only a few cells per milliliter of blood, which makes their detection challenging. (wikipedia.org)
Apoptotic CTCs are traditional CTCs that are undergoing apoptosis (programmed cell death). (wikipedia.org)
Small CTCs are cytokeratin-positive and CD45-negative, but with sizes and shapes similar to white blood cells. (wikipedia.org)
DOI - https://doi.org/10.18632/oncotarget.27510 Correspondence to - Michael R. King - [email protected] Abstract Previous studies have demonstrated that CTCs do not travel in the bloodstream alone, but rather are accompanied by clusters of stromal cells such as cancer associated fibroblasts (CAFs). (labtube.tv)
The observation that CAFs disseminate with CTCs prompts the examination of the role of CAFs in CTC survival under physiological shear stress during the dissemination process using a clinically relevant, three-dimensional (3D) co-culture model. (labtube.tv)

Uncovering key kinases in tumor growth and invasion is crucial for improving targeted therapies in advanced-stage colorectal cancer. (medindia.net)
For VEGF, the target of current therapies, this correlation between hypoxia and higher cytokine levels was greater in primary breast and lung carcinoma cells than in ovarian carcinoma cells or tumor cell lines. (biomedcentral.com)
Clinical trials of LAK cells in humans are ongoing but this approach has not gained widespread use and is generally considered less effective than other cell therapies. (msdmanuals.com)

Organoids, termed "mini-organs" by many, are comprised of organ-specific cell types (from stem cells or progenitor cells) that exhibit lineage-specific differentiation and self-assembly. (labmanager.com)
Therefore, many authorities suggest pluripotential progenitor cells, such as undifferentiated mesenchymal cells, may be the origin of DFSP, because they have the capacity to differentiate into all 3 cell types. (medscape.com)
62) also observed CK19+ oblong cells in HCV-positive liver cirrhosis (47) and coexpression of CK19 and AFP in hepatic progenitor cells. (suamaytinhhaiphong.com)
Tet lo demonstrated precursor exhausted or progenitor T cell phenotypes marked by higher expression of Tcf-1 and T-bet, and lower expression of the exhaustion markers CD39, PD-1 and Eomes compared with Tet hi , whereas Tet hi cells were terminally exhausted. (bmj.com)

Angiogenesis is a balanced process controlled by pro- and anti-angiogenic molecules of which the regulation is not fully understood. (plos.org)
Altogether, by identifying a pro-angiogenic VEGF/miR-101/EZH2 axis in endothelial cells we provide evidence for a functional link between growth factor-mediated signaling, post-transcriptional silencing, and histone-methylation in the angiogenesis process. (plos.org)
Angiogenesis is a balanced process controlled by pro- and anti-angiogenic molecules [3] . (plos.org)
Novel cancer treatment strategies take advantage of tumor-induced vascularisation by combining standard chemotherapeutic agents with angiogenesis-inhibiting agents. (biomedcentral.com)

We previously showed that VEGF signaling in primary endothelial cell cultures caused overexpression of VEGFR2 in a positive feed-forward loop, which is at least partly regulated by loss of miRNA-mediated control of VEGF receptor degradation. (plos.org)
The aim of this work was to study the protective activity of a honey of multifloral origin, standardized for total antioxidant power and analytically profiled (HPLC-MS) in antioxidants, in a cultured endothelial cell line (EA.hy926) subjected to oxidative stress. (researchgate.net)
Experiments with endothelial cells fortified with the isolated fraction from native honey enriched in antioxidants, exposed to peroxyl radicals from 1,1-diphenyl-2-picrylhydrazyl (AAPH, 10 mM) and to hydrogen peroxide (H2O2, 50-100 microM), indicated that phenolic acids and flavonoids were the main causes of the protective effect. (researchgate.net)
It may also invade and proliferate within heart and coronary artery endothelial cells, and, along with Streptococcus sanguis, it may also induce platelet aggregation associated with thrombus formation. (medscape.com)

T-cell engagers are bispecific antibodies that recruit cytotoxic T cells to kill tumor cells. (msdmanuals.com)

Thus, their reactivation represents an attractive way to unleash anti-tumour immunity. (portlandpress.com)

This follows a license agreement with SyntArray, LLC, announced on March 13th , and is the latest milestone in the Company's strategy to bring together ground-breaking technologies to develop the next generation of patient derived ("PDx") tumor models for precision cancer therapy and drug development. (globenewswire.com)
In addition to its proprietary precision diagnostics for oncology, Helomics offers boutique CRO services that leverage our patient-derived tumor models, coupled to a wide range of multi-omics assays (genomics, proteomics and biochemical), and a proprietary bioinformatics platform (D-CHIP) to provide a tailored solution to our client's specific needs. (globenewswire.com)
Patient-derived glioma stem cells (green) invading and proliferating within a miniature replica of the human brain. (labmanager.com)
The researchers also extracted mutational signatures from each patient-derived cell line and found eight base substitution mutational signatures, including seven that have been previously described and one that was novel. (genomeweb.com)
3D patient-derived tumor organoids hold great potential for many basic and translational applications. (selectscience.net)

This allowed them to analyze interactions between the two cell populations and perform unique, accurate measurements of cell positions and the spatiotemporal process of immune response. (pasteur.fr)
[ 2 ] In addition, some tumor types do not appear to adhere to the CSC concept at all [ 3 , 4 ] or may have multiple independent tumor-initiating cell populations. (medscape.com)

We named this cerebral organoid glioma model the "GLICO" model and published our findings in Cell Reports . (labmanager.com)
Since our 3D models required gentle agitation within an incubator in order to remain viable, there was a lot of trial and error to determine the best way to effectively image the tumor and return the organoid to a shaking culture environment, without affecting the viability of the sample itself. (labmanager.com)
The researchers dissected colorectal cancers from three previously untreated individuals and derived organoid cultures from them. (genomeweb.com)
However, traditional approaches to organoid image analysis and downstream data processing are o. (selectscience.net)

Here, we employ high-resolution intravital microscopy using a CSC biosensor to directly observe CSCs in live mice with mammary tumors. (nature.com)
At the apex of the hierarchy are the cancer stem cells (CSCs). (nature.com)
[ 5 ] Obviously, the study of CSCs in any given tumor type, by proteomics or other approaches, requires prior validation of their actual existence. (medscape.com)

Methods CT26 models were treated with anti-PD-1 on days 3, 6 and 9 following subcutaneous tumor implantation generating variable responses during early tumor development. (bmj.com)

The Western blot and immunohistochemical analyses of tumor xenografts validated this assumption. (suamaytinhhaiphong.com)
These HCV-caused stem cell-like features were clearly absent in Huh7.5 culture cells as well as their tumor xenografts. (suamaytinhhaiphong.com)

Furthermore the sarcomatoid elements demonstrate an enhanced expression of epithelial-mesenchymal transition related mesenchymal markers as compared with the clear cell renal cell carcinoma counterparts. (lu.se)

Tetramer staining was performed to determine the frequency and avidity of CD8 + T cells targeting the tumor-specific epitope GSW11 and confirmed with tetramer competition assays. (bmj.com)

The numbers of mutations of several signatures differed markedly between individual branches [of the phylogenetic tree], indicating varying contributions of mutational processes in different parts of the cancer. (genomeweb.com)
Using 6 biphasic clear cell renal cell carcinoma cases we show that sarcomatoid clear cell renal cell carcinoma shares characteristic markers associated with loss of von Hippel-Lindau tumor suppressor with conventional clear cell renal cell carcinoma and also exhibits a markedly higher proliferative index. (lu.se)

Emerging evidence supports that senescence is an important tumor suppressor. (umassmed.edu)

NK cells express germ-line receptors that are either stimulatory or inhibitory, and the summation of these signals determines activation status ( Caligiuri, 2008 ). (elifesciences.org)
Besides, NGF binding to TrkA receptors encouraged the intracellular Warburg effect in PC and DRG cells. (hindawi.com)

Tumor Antigens Many tumor cells produce antigens, which may be released in the bloodstream or remain on the cell surface. (msdmanuals.com)
Concomitant use of interferon enhances the expression of major histocompatibility complex (MHC) antigens and TAAs on tumor cells, thereby augmenting the killing of tumor cells by the infused effector cells. (msdmanuals.com)
Tests such as ELISpot enumerate cells releasing IFN-g after mononuclear cells recovered from whole blood are incubated with similar antigens. (cdc.gov)

We have previously shown mitotic spindle aberrations in cultured primary and immortalized human airway epithelial cells exposed to single-walled carbon nanotube s (SWCNT). (cdc.gov)
Immortalized and primary respiratory epithelial cells were exposed to 0.024, 0.24, 2.4, and 24 microg/cm2 carbon nanotube s. (cdc.gov)
There was a mean of one genome rearrangement in clonal organoids derived from normal colorectal epithelial cells compared with means in cancer-derived clonal organoids of 71 rearrangements from patient one, 176 from patient two, and 67 from patient three. (genomeweb.com)
Sarcomatoid conversion of clear cell renal cell carcinoma in relation to epithelial-to-mesenchymal transition. (lu.se)
In the present study we investigate whether the sarcomatoid conversion of clear cell renal cell carcinoma could be interpreted as linked to the process of epithelial-mesenchymal transition. (lu.se)
We further selected a representative case, clinically demonstrating direct overgrowth of the sarcomatoid component into the liver and colon, for extended immunohistochemical characterization, resulting in a further set of positive and negative epithelial-mesenchymal transition markers as well as pronounced transforming growth factor β positivity, indicating that sarcomatoid clear cell renal cell carcinoma may be associated to epithelial-mesenchymal transition. (lu.se)
We therefore suggest that sarcomatoid clear cell renal cell carcinoma morphologically and immunohistochemically may represent a completed epithelial-mesenchymal transition and that transforming growth factor β1 could be an important driving force during the sarcomatoid transdifferentiation of clear cell renal cell carcinoma. (lu.se)
The same age differential is noted with respect to the more common odontogenic tumors (eg, ameloblastoma, odontoma, adenomatoid odontogenic tumor, calcifying epithelial odontogenic tumor, ameloblastic fibroma, odontogenic myxoma, odontogenic fibroma). (medscape.com)

Further, exosomes were extracted from PC cells and identified by examining the levels of specific markers for exosomes. (hindawi.com)
Both 2D monolayer and 3D neurospheres were successfully cultured and characterized for proliferative potential, intrinsic plasticity, presence of cancer stem cells and the expression of stem cell markers. (frontiersin.org)

However, this finding has triggered further interest in utilising tumour-specific MHC-II to harness sustained anti-tumour immunity through the activation of CD4 + T helper cells. (portlandpress.com)
The reactive CAFs showed higher expression of α-smooth muscle actin (α-SMA) and fibroblast activation protein (FAP) compared to differentiated CAFs, when co-cultured with PC cells at the same experimental conditions. (labtube.tv)

Tumour-specific expression of MHC-I and MHC-II has been associated with improved patient survival in most clinical studies. (portlandpress.com)

significant cytotoxicity was observed in the MWCNT-exposed cells. (cdc.gov)
The addition of essential amino acids, but not non-essential amino acids to the cell culture medium resulted in the cancellation of this pronounced cytotoxicity. (spandidos-publications.com)
Autophagic cell death was excluded as the inhibition of autophagy did not attenuate the cytotoxicity. (spandidos-publications.com)

Arrows indicate patterns of tumor organization that are consistent with collective invasion. (jci.org)
This is consistent with the role played by mitochondria in supporting cell motility, previously described by the Altieri lab. (wistar.org)

In the case of cerebral organoids, each stage of the differentiation process is dependent on timing. (labmanager.com)
In a paper published today in Nature , researchers from the UK, the Netherlands, and the US described their efforts to characterize organoids derived from multiple single cells from three colorectal cancers as well as from adjacent normal intestinal crypts. (genomeweb.com)

It is well known that the rapid growth of tumor cells and distortion of tumor blood vessels often results in insufficient oxygen supply and acidification in solid tumors. (nature.com)
If one administers the appropriate growth factor or biological cue, but at the inappropriate time, then there can be wide variation in the resulting cell types that are generated. (labmanager.com)
Treatment of syngeneic tumors resulted in CD8 and PD-L1-dependent tumor rejection or growth inhibition and a reduction in myeloid cells endogenously expressing high levels of PD-L1. (elifesciences.org)
Treatment of xenograft tumors resulted in PD-L1-dependent tumor growth inhibition. (elifesciences.org)
Cultured DFSP tumor cells have increased growth in response to platelet-derived growth factor (PDGF)-beta. (medscape.com)
A localized hypoxic environment occurs during tumor growth necessitating an angiogenic response or tumor necrosis results. (biomedcentral.com)
Since epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitors, including gefitinib (GEF) have been reported to induce the apoptosis of several cancer cell lines, in the present study, we examined whether the cytotoxic effects of GEF are further enhanced under amino acid starvation (AAS) culture conditions. (spandidos-publications.com)
Although melanotic neuroectodermal tumor of infancy (MNTI) is classified as a benign lesion, it is often clinically worrisome because of its rapid onset and alarming local growth rate. (medscape.com)

Consider clinical, radiographic, laboratory, and histologic findings when establishing a proper differential diagnosis for melanotic neuroectodermal tumor of infancy (MNTI). (medscape.com)

Tumorigenesis is a multi-step process in which a normal cell acquires changes in a number of critical cancer causing genes. (umassmed.edu)
Senescence limits proliferative capacity of cells and thus impedes the accumulation of multiple mutations necessary for tumorigenesis. (umassmed.edu)

Here, we identified an epigenetically distinct subpopulation of breast tumor cells that have an enhanced capacity to collectively invade. (jci.org)

The process causes little damage to the cells, which benefits subsequent cell cultures. (chemistryviews.org)

By contrast, analysis of solid tumors necessitates invasive procedures that might limit patient compliance. (wikipedia.org)
Solid arrows indicate where collagen I is being reorganized into parallel tracks by trailblazer cells. (jci.org)
Solid arrows indicate a leading cell. (jci.org)
DIG is a grade I tumor involving superficial cerebral cortex and leptomeninges, often attached to dura, with a large mass composed of both solid and cystic areas. (frontiersin.org)
Disruption of the centrosome is common in many solid tumors including lung cancer. (cdc.gov)
We demonstrated that both GS5 and Huh7.5 cells could form solid tumors in athymic nude rodents. (suamaytinhhaiphong.com)
Perspectives of tumor-infiltrating lymphocyte treatment in solid tumors. (msdmanuals.com)

Our technical expertise include lentiviral vectors, transgenic mice and stem cells cultures. (lu.se)

A circulating tumor cell (CTC) is a cell that has shed into the vasculature or lymphatics from a primary tumor and is carried around the body in the blood circulation. (wikipedia.org)
Fifty cell sources, including both immortalized cell lines and primary carcinoma cells, were incubated under normoxic conditions for 48 hours. (biomedcentral.com)

In this study, we found that "reactive CAFs" induce shear resistance to prostate tumor cells via intercellular contact and soluble derived factors. (labtube.tv)

Anatomy21

Organisms11

Diseases79

Chemicals and Drugs50

Analytical, Diagnostic and Therapeutic Techniques and Equipment51

Phenomena and Processes49

Disciplines and Occupations1

Information Science4

Health Care6

Microenvironment8

Metastasis11

Vitro11

Glioblastoma6

Proliferation10

Pathways7

Tissue18

Proteins6

Recurrence5

Progression8

Murine1

Cellular14

Breast tumors1

Vivo9

Lymphocytes3

Brain tumors2

Patient's9

Biology3

Phenotype6

Therapeutic2

Cancer cells42

Tissues4

Malignant3

Heterogeneity2

Dendritic cells10

Aggressive2

Patients13

Metastatic3

CTCs6

Therapies3

Progenitor4

Angiogenesis4

Endothelial4

Cytotoxic T cel1

Reactivation1

Patient-derived5

Populations2

Organoid4

CSCs3

Methods1

Xenografts2

Mesenchymal markers1

Assays1

Markedly2

Suppressor1

Receptors2

Antigens3

Epithelial8

Markers2

Activation2

Survival1

Cytotoxicity3

Consistent2

Organoids2

Growth8

Laboratory1

Tumorigenesis2

Subpopulation1

Subsequent1

Solid7

Mice1

Primary2

Induce1