AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsInformation ScienceHealth Care
Frozen SectionsMicrotomyIntraoperative PeriodPathology, SurgicalCesarean SectionParaffinHistological TechniquesTelepathologyHistocytochemistryImmunoenzyme TechniquesConizationFluorescent Antibody TechniqueFalse Negative ReactionsStaining and LabelingParaffin EmbeddingIntraoperative CareImmunohistochemistrySentinel Lymph Node BiopsySensitivity and SpecificityDiagnostic ErrorsDiagnostic Techniques, SurgicalBiopsyLymph NodesAntibodies, MonoclonalMicroscopy, ElectronFreezingTolonium ChlorideRetrospective StudiesBiopsy, NeedleCytodiagnosisEyelid NeoplasmsFixativesBreast NeoplasmsMohs SurgeryTime FactorsSpecimen HandlingTissue PreservationLymphatic MetastasisCarcinoma, LobularTissue FixationImmunologic TechniquesTreatment OutcomeOsmium TetroxideMicrodissectionFluorescent Antibody Technique, IndirectKeratinsHysterectomyLymph Node ExcisionCarcinomaDissectionReproducibility of ResultsFalse Positive ReactionsEpitheliumMethodsAutoradiographyAxillaPelvisBreastFormaldehydeAntigens, NeoplasmFollow-Up StudiesProspective StudiesMonitoring, IntraoperativePregnancyThyroid NoduleColoring AgentsCarcinoma, Basal CellMicroscopy, FluorescenceMicroscopy, ImmunoelectronBiopsy, Fine-NeedleRabbitsSurgical Procedures, OperativeLasersPulmonary Sclerosing HemangiomaMediastinoscopyParathyroidectomyPredictive Value of TestsCarcinoma, Ductal, Breast
IntraoperativeCryostatFFPEFixationParaffin embedded sectionsSpecimenBiopsyStored under appropriate frozenTumorSpecimensExcisionDiagnosisTissue sectionsSlidesBiopsiesImmunohistochemicalTissuesSpleen2023XyleneHemorrhageLaboratoryAvoidProvide sufficientSurgical2016ExaminationAntibodiesAccordanceTherapeuticFindingsRepositoryAntibodyGuidancePatternsConditionsProblemFeedbackLaboratoriesAccessibilityPatientsCompoundObjectivesMethodsApplicationsPreventCellsObtainManagePurposeControlBlocksCommonFollowTimeLong
Intraoperative3
  • The intraoperative consultation is the name given to the whole intervention by the pathologist, which includes not only frozen section but also gross evaluation of the specimen, examination of cytology preparations taken on the specimen (e.g. touch imprints), and aliquoting of the specimen for special studies (e.g. molecular pathology techniques, flow cytometry). (wikipedia.org)
  • Angiosarcoma may present a challenge in intraoperative frozen section diagnosis of an ovarian mass. (hindawi.com)
  • The purpose of this report was to describe the features of angiosarcoma arising in mucinous tumor that was initially misinterpreted as a nonneoplastic vascular proliferation in the intraoperative frozen section examination. (hindawi.com)
Cryostat2
  • Subsequently, it is cut frozen with the microtome portion of the cryostat, the section is picked up on a glass slide and stained (usually with hematoxylin and eosin, the H&E stain). (wikipedia.org)
  • Cut 4-8 um thick cryostat sections and mount on superfrost plus slides or gelatin coated slides. (ihcworld.com)
FFPE6
  • The QIAamp DNA FFPE Tissue Kit is specially designed for purifying DNA from formalin-fixed, paraffin-embedded tissue sections. (qiagen.com)
  • Specially optimized lysis conditions allow genomic DNA to be efficiently purified from FFPE tissue sections without the need for overnight incubation. (qiagen.com)
  • Note that DNA isolated from FFPE samples is usually of lower molecular weight than DNA from fresh or frozen samples. (qiagen.com)
  • After sample lysis, the simple QIAamp DNA FFPE Tissue procedure, which is highly suited for simultaneous processing of multiple samples, yields pure DNA in less than 30 minutes. (qiagen.com)
  • I am attempting one on muscle frozen sections (my control is FFPE) and I am not sure what results to expect. (utsouthwestern.edu)
  • 1. Isolation of DNA from tumor biopsies, paraffin-embedded sections (FFPE), fresh frozen tumors, or plasma (for cell free kit). (dssimage.com)
Fixation2
  • Wilson developed the technique from earlier reports at the request of Dr William Mayo, surgeon and one of the founders of the Mayo Clinic Earlier reports by Dr Thomas S. Cullen at Johns Hopkins Hospital in Baltimore also involved frozen section, but only after formalin fixation, and pathologist Dr William Welch, also at Hopkins, experimented with Cullen's procedure but without clinical consequences. (wikipedia.org)
  • Students will perform histological procedures involving tissue specimen including: fixation, embedding, microtomy, frozen sectioning, and staining paraffin embedded tissue. (asurams.edu)
Paraffin embedded sections1
Specimen5
  • The frozen section procedure is a pathological laboratory procedure to perform rapid microscopic analysis of a specimen. (wikipedia.org)
  • Detailed specimen collection and processing instructions are discussed in the NHANES Laboratory/Medical Technologists Procedures Manual (LPM). (cdc.gov)
  • Detailed instructions on specimen collection and processing are discussed in the NHANES 2017-2018 and 2019-2020 Laboratory Procedures Manuals (LPMs). (cdc.gov)
  • Freeze specimen as soon as possible (−70 °C or dry ice preferred). (cdc.gov)
  • Label specimens with labels generated by your facility and follow your facility's procedures for proper specimen labeling. (cdc.gov)
Biopsy3
  • A bone biopsy is a procedure in which a small sample of bone is taken from the body and looked at under a microscope for cancer, infection, or other bone disorders. (healthlinkbc.ca)
  • Although neuroendoscopic biopsy is routinely performed, the safety and validity of this procedure has been studied only in small numbers of patients in single-center reports. (thejns.org)
  • This is one of the largest series confirming the safety and validity of the neuroendoscopic biopsy procedure. (thejns.org)
Stored under appropriate frozen1
  • Vials were stored under appropriate frozen (-20 degrees Centigrade) conditions until they were shipped to University of Minnesota for testing. (cdc.gov)
Tumor5
  • The most common indication for a frozen section is to determine whether surgical margins are free of tumor (ie, to assess the adequacy of tissue excision). (medscape.com)
  • The frozen section diagnosis was at least mucinous borderline tumor. (hindawi.com)
  • The frozen section diagnosis was at least mucinous borderline tumor, pending adequate tissue sampling for permanent sections to rule out mucinous adenocarcinoma. (hindawi.com)
  • PM consists of frozen tissue section of primary tumor and corresponding metastatic tumor. (biochain.com)
  • Immunohistochemistry procedure uses direct labeled antibodies against particular proteins specific to certain tumor types. (breachcandyhospital.org)
Specimens1
  • For microtensile test, samples were prepared into blocks of enamel/resin, which were sectioned to obtain hourglass-like specimens. (bvsalud.org)
Excision1
  • FSIS currently collects raw beef manufacturing trimmings verification samples using the N60 excision sampling procedure as described in FSIS Directive 10,010.1 , Sampling Verification Activities for Shiga Toxin-Producing Escherichia coli (STEC) in Raw Beef Products . (usda.gov)
Diagnosis2
  • A frozen section should not be used for an "on-the-spot" diagnosis, since frozen tissue rendered tissue morphology is less optimal for microscopic examination. (medscape.com)
  • Our Surgical Pathology Department also performs diagnoses using frozen sections if an urgent diagnosis is needed. (breachcandyhospital.org)
Tissue sections4
  • FD NeuroApop™ Kit is specifically designed for the detection of neuronal apoptosis in tissue sections from the central nervous system based on the principle of in situ DNA nick-end labeling (TUNEL) technique. (thomassci.com)
  • The kit uses special lysis conditions to release DNA from tissue sections and to overcome inhibitory effects caused by formalin crosslinking of nucleic acids. (qiagen.com)
  • The frozen tissue sections in each pair are prepared from the same donor. (biochain.com)
  • Tissue sections were evaluated on a Leica DMLB microscope. (cdc.gov)
Slides3
  • The quality of the slides produced by frozen section is of lower quality than formalin fixed paraffin embedded tissue processing. (wikipedia.org)
  • BioChain's Frozen Tissue Slides are ideal for rapidly identifying cellular localization of RNA or protein. (biochain.com)
  • Transverse sections (12 um) were cut, mounted on pre-coated microscope slides, and stained using a routine procedure with Harris Hematoxylin and Eosin. (cdc.gov)
Biopsies1
  • We do Highman's Congo Red on frozen muscle biopsies that have been fixed in Baker's Formol Calcium for at least 10 minutes. (utsouthwestern.edu)
Immunohistochemical3
  • The M5E2 antibody is recommended to test for immunohistochemical staining of acetone-fixed frozen sections. (bdbiosciences.com)
  • For optimal indirect immunohistochemical staining, the M5E2 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with Biotin anti-mouse IgG2a (Cat. (bdbiosciences.com)
  • This antibody may be used for immunohistochemical staining of acetone-fixed, frozen and zinc-fixed, paraffin sections. (bdbiosciences.com)
Tissues2
  • Snap frozen fresh tissues in liquid nitrogen or isopentane pre-cooled in liquid nitrogen, embedded in OCT compound in cryomolds. (ihcworld.com)
  • What procedures do you use to keep human tissues viable until you can cryogenically preserve them? (alcor.org)
Spleen1
  • A frozen section from rat spleen was stained with Mouse Anti-Rat CD11b/c (Cat.550299). (bdbiosciences.com)
20231
  • You can return the products to the store, and a refund procedure will run until Saturday, November 25, 2023. (connexionfrance.com)
Xylene1
Hemorrhage2
  • The hemorrhagic area, which was intraoperatively interpreted as organizing vessels associated with previous hemorrhage, represented angiosarcoma in permanent sections. (hindawi.com)
  • The hemorrhagic area sampled in the frozen section was interpreted as organizing vessels associated with intratumoral hemorrhage (Figure 1(b) ). (hindawi.com)
Laboratory2
  • Refer to the Laboratory Method Files section for a detailed description of the laboratory methods used. (cdc.gov)
  • NCHS and contract consultants use a structured competency assessment evaluation during visits to evaluate both the quality of the laboratory work and the QC procedures. (cdc.gov)
Avoid2
Provide sufficient1
  • Stroke Risk in Medically Treated Asymptomatic Carotid Stenosis Should patients with asymptomatic carotid artery stenosis undergo carotid procedures, or does medical therapy provide sufficient protection against stroke? (medscape.com)
Surgical2
  • A failure to intraoperatively recognize this malignant neoplasm may result in incomplete surgical staging procedure or inappropriate clinical management. (hindawi.com)
  • Little Samuel's mother, Julie Armas, is an obstetrics nurse in Atlanta She knew of Dr. Bruner's remarkable surgical procedure. (emofree.com)
20164
  • Hou Zhili arrives at the end of the Irtysh's Kazakhstan section on Sept. 13, 2016. (xinhuanet.com)
  • Setting sail from his home in Fuyun County, northwest China's Xinjiang Uygur Autonomous Region, the 41-year-old has completed what many see as epic feats -- kayaking unpowered for a total of 2,020 kilometers on the China section and Kazakhstan section of the Irtysh in 2014 and in 2016. (xinhuanet.com)
  • On Sept. 20, 2016, Hou arrives at Lake Markakol on his way back after finishing his mission on the Kazakhstan section of the Irtysh. (xinhuanet.com)
  • Kazakhstan has facilitated visa procedures for Chinese tourists since in 2016. (xinhuanet.com)
Examination1
  • The principal use of the frozen section procedure is the examination of tissue while surgery is taking place. (wikipedia.org)
Antibodies1
  • In non-fixed brain sections these antibodies gave labeling patterns similar to autoradiographic distributions with particularly high levels in the hippocampus. (jneurosci.org)
Accordance3
  • Therefore, in accordance with section 751(a)(3)(A) of the Act, the Department has decided to extend the time limit for the preliminary results from 245 days to 365 days. (justia.com)
  • This notice is issued and published in accordance with sections 751(a)(3)(A) and 777(i)(1) of the Act. (justia.com)
  • Dispose in accordance with Federal, State, and Local regulations and procedures. (crest-ultrasonics.com)
Therapeutic1
Findings1
  • The findings can guide a procedure and can immediately be shared with the surgeon while the patient is still in the operating room. (breachcandyhospital.org)
Repository2
  • BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. (biochain.com)
  • The later "repository" sections describe installing specific drivers. (opensuse.org)
Antibody3
  • Frozen sections of normal human tonsil was reacted with the M5E2 antibody. (bdbiosciences.com)
  • incubate sections with normal serum block - species same as secondary antibody, for 30 minutes to block non-specific binding of immunoglobulin. (ihcworld.com)
  • incubate sections with biotinylated secondary antibody at appropriate dilution in PBS for 30 minutes at room temperature. (ihcworld.com)
Guidance5
  • This IRM section provides general guidance for employees who have contact with taxpayers who may inquire about innocent spouse relief. (irs.gov)
  • Guidance regarding Sub-sections 4.6, 4.8 and 6.4 of the SmPC are given below. (who.int)
  • Guidance for Sections 4.6 and 6.4 is based on the European Medicines Agency's Guideline on Risk Assessment of Medicinal Products on Human Reproduction and Lactation: from Data to Labelling. (who.int)
  • Guidance for Section 4.8 is based on MedDRA1 guidance. (who.int)
  • In this regard, the Network was of the view that further guidance was needed from the Commission on the allowances that would be subject to a freeze and those that were considered to be monetary or non-monetary in order to ensure that a fact- based, objective and dependable comparison was carried out. (who.int)
Patterns1
  • Patterns of Major Frozen Section Interpretation Error This study aimed to establish data error rates due to misinterpretation of frozen sections at a large medical center. (medscape.com)
Conditions1
  • Unless weather conditions require a different approach, winter maintenance routes are treated with a mixture of sand and salt, generally with a minimum amount of salt to prevent freezing of the mixture. (austellga.gov)
Problem3
  • Find support for a specific problem in the support section of our website. (mdpi.com)
  • I think the system freezing problem is resolved as well although I restarted my PC and keep it running for only one hour till now. (opensuse.org)
  • In 2015, when it was high time for the journey on the Kazakhstan section, his plan was postponed due to a visa problem. (xinhuanet.com)
Feedback1
  • testing procedures and constructive feedback are given to each staff member. (cdc.gov)
Laboratories2
  • The frozen section procedure as practiced today in medical laboratories is based on the description by Dr Louis B. Wilson in 1905. (wikipedia.org)
  • As with all procedures, laboratories should perform a site-specific and activity-specific risk assessment to identify and mitigate risks. (cdc.gov)
Accessibility2
  • Converted several Tables, charts, and images throughout the Code to meet web accessibility requirements under Section 508 of the Rehabilitation Act of 1973 (29 U.S.C. 794d). (fda.gov)
  • CDC is not responsible for Section 508 compliance (accessibility) on other federal or private website. (cdc.gov)
Patients2
  • Only 2 patients required a ventriculoperitoneal shunt shortly after the endoscopy procedure because ETV was not feasible. (thejns.org)
  • Patients and Methods: Seventeen patients of recto-sigmoid Hirschsprung's disease underwent single-stage transanal Swenson's procedure. (ajol.info)
Compound1
  • this compound is known by many names and when frozen has the same density as frozen tissue. (wikipedia.org)
Objectives1
Methods1
  • The analytical methods are described in the Analytic methodology section. (cdc.gov)
Applications1
  • Augmented Reality Applications in Orthopedic Spine Surgery This review explores the potential role of augmented reality in the setting of spinal procedures. (medscape.com)
Prevent2
  • A numbing medicine ( local anesthetic ) is used to prevent pain during this procedure. (healthlinkbc.ca)
  • One or more of the following, which may delay or prevent the implementation of this policy, may affect all or any part of normal operating procedures. (austellga.gov)
Cells2
Obtain1
Manage1
  • The Human Resources Network noted the decisions of the General Assembly to request the Commission to actively manage the margin using established margin management procedures, and to refrain from increasing any of the allowances under its purview until the present review had been submitted to the General Assembly for consideration. (who.int)
Purpose1
  • The freezing period can be extended for an additional period of 6 months, provided the claimant submits a formal request for this purpose. (dreyfus.fr)
Control3
  • The objective stated in Section 1 may be best achieved by implementation and execution of the procedures and tasks outline in Section 2 of this policy, Winter Operations Tree Removal and Ice Control. (austellga.gov)
  • This policy, including the standard operating procedure, is intended to serve as the normal procedure for winter maintenance and ice control for the City of Austell. (austellga.gov)
  • Winter Operations Tree Removal and Ice Control Procedures. (austellga.gov)
Blocks2
  • The microtome device that cold cuts thin blocks of frozen tissue is called a cryotome. (wikipedia.org)
  • Store frozen blocks at - 80 ºC. (ihcworld.com)
Common1
  • Is cryonics unique or does it have anything in common with low-temperature surgery or freezing sperm or eggs? (alcor.org)
Follow2
  • Follow procedure for pretreatment as required. (ihcworld.com)
  • The City of Austell is bound by law to follow certain legal requirements and procedures that may delay immediate availability of funds for responses. (austellga.gov)
Time2
  • I notice that every time when PC freezes, the Xorg occupies 100% cpu resouce and as long as keeping this status. (opensuse.org)
  • You can change your preferences at any time by clicking on the "Customise my choices" section. (dreyfus.fr)
Long3
  • The paddler is preparing for the final and most challenging stage on the 2,200-kilometer long Russia section, plus a 1,000-kilometer long lower reach section of the Ob River to reach the Arctic Ocean. (xinhuanet.com)
  • He paddled for 50 kilometers a day, and took 23 days to arrive at Kaba County, where the 520-kilometer long China section ends. (xinhuanet.com)
  • It took him 33 days to finish rowing the 1,500-kilometer long section. (xinhuanet.com)