AntibodiesAntibodyAntigenProteinsMolecularViralPeptidesAntigensCharacterizationCrypticAminoPotentImmuneGenerateAssayProtein sequenceStructuralSpecificityGenesRecognizeInteractomeSensitivityProteomicsTargetHumanBoundMoleculesTherapeuticCellMembraneMethodInvolvesSpecificProductionFunctionalResearchMultipleProtocolsDevelopConditions
Antibodies10
- Regions on the antigen which are recognized by the antibodies are called epitopes, and the respective molecular counterpart of the epitope on the mAbs is called paratope. (bvsalud.org)
- Also, it is a popular method for epitope mapping and screening antibodies for target specificity. (web.app)
- The antibodies are also ideally suited for use in immunohistochemistry procedures (see figure Immunohistochemistry with Penta·His Antibodies. . "> "Immunohistochemistry with Penta·His Antibodies" ). (qiagen.com)
- High resolution mass spectrometry is employed for epitope mapping of antibodies and identification, quantification and characterization of proteins and peptides including determination of phosphorylation and glycosylation sites and optionally their structure. (proteome-factory.com)
- While polyclonal antibodies are secreted by many different B cell clones and recognize multiple antigenic epitopes, monoclonals originate from a single B cell clone and are specific for just one epitope. (cellsignal.com)
- The anti-GBM antibodies can target the EA and EB epitope separately. (medscape.com)
- The synthesis and applications of the peptides are gaining increasing popularity as a result of the developments in biotechnology and bioengineering areas and for a number of research purposes including cancer diagnosis and treatment, antibiotic drug development, epitope mapping, production of antibodies, and vaccine design. (intechopen.com)
- We applied FLIP to the screening of mouse monoclonal antibodies of unknown behavior in IP procedures. (biomedcentral.com)
- The parallel analysis of the considered antibodies using FLIP and IP/western shows good correlation between the two procedures. (biomedcentral.com)
- Until now the standard procedure to validate antibodies for IP couples the IP assay to immunoblotting (IP/IB), a procedure that is challenging to scale to high-throughput analysis [ 10 ]. (biomedcentral.com)
Antibody7
- Twenty-five years later, the Pepscan methodology, evolved and modernized with the latest insights, is still an important part of Pepscan's epitope mapping platform, which is instrumental in therapeutic antibody development. (wikipedia.org)
- In this review, we describe a fast high-throughput antibody (scFv) selection procedure in 96-well microtiter plates. (bvsalud.org)
- In this work, we isolate a dromedary camel V H H single-domain antibody (also known as nanobody) named D4 and a monoclonal antibody (mAb) named HM2 recognizing membrane-distal and membrane-proximal epitopes of GPC1, respectively. (nature.com)
- [ 5 ] The more abundant D-hexamers have dimer-reinforced crosslinks between NC1 domains that help to retain the cryptic nature of the Goodpasture epitopes, whereas the less abundant M-hexamers, composed of only monomeric subunits, allow epitope unmasking and antibody binding under inflammatory states. (medscape.com)
- IP and ChIP are both inexpensive and highly informative, relying on a specific antibody to selectively bind to the target epitope with high affinity [ 5 ]. (biomedcentral.com)
- For instance, for some IP applications the antibody should recognize the target protein in its three-dimensional folded state, which requires that the epitope be exposed on an accessible surface of the target protein [ 7 ]. (biomedcentral.com)
- If your Custom Antibody Production project must be free of mycoplasma or viral contamination, we recommend our mouse antibody production (MAP), rat antibody production (RAP) and hamster antibody production (HAP) testing programs. (inotivco.com)
Antigen2
- Finally, antigen identification does not require knowledge of the restriction element and often leads directly to the identification of the T-cell epitope. (nih.gov)
- Various specific mechanisms (etiologies) of autoimmunity are recognized as plausible in viral induction of autoimmunity, including epitope spreading, molecular mimicry, cryptic antigen, and bystander activation, each a plausible etiological mechanism responsible for activating self-reactive (SR) immune cells. (danielsolis.cz)
Proteins8
- It represents a simplification of the western blot method, with the exception that the proteins to be detected are not first separated by electrophoresis.Instead, the sample is applied directly on a membrane in a single spot, and the blotting procedure is performed. (web.app)
- SAR-CoV-2 spike proteins, and all other SARS-CoV-2 proteins, immunogenic epitopes in each SARS-CoV-2 protein were compared to human proteins in search of high local homologous matching. (danielsolis.cz)
- Only one immunogenic epitope in a SARS-CoV-2 had no homology to human proteins. (danielsolis.cz)
- If all of the parts of the epitopes that are homologous to human proteins are excluded from consideration due to risk of pathogenic priming, the remaining immunogenic parts of the epitopes may be still immunogenic and remain as potentially viable candidates for vaccine development. (danielsolis.cz)
- We initially used maps statically to generate a database with localization and absolute copy number information for over 8700 proteins from HeLa cells, approaching comprehensive coverage. (elifesciences.org)
- The resulting technique maps the location of most of the proteins in a human cancer cell line and, in addition, determines how many copies of each protein there are. (elifesciences.org)
- Though the group did the mapping with ABC transporters from a yeast species - Saccharomyces cerevisiae - many of the proteins are homologous to disease-linked human ABC proteins. (genomeweb.com)
- Overall, the study yielded a map covering 537 unique binary interactions across 366 proteins, the study authors wrote, which they annotated with functional classifications to create a standard map, as well as a map showing protein conservation in humans and known disease associations. (genomeweb.com)
Molecular3
- BP - natural procedures, CC - mobile parts, MF C molecular function. (gasyblog.com)
- BP - natural procedures, CC - mobile parts, MF NGD-4715 C molecular Mouse monoclonal to FAK function. (gasyblog.com)
- We use multiple functional brain mapping techniques (fMRI, diffusion tensor imaging, and intracranial EEG) and structural and molecular imaging techniques to better define individual functional anatomy in patients with neurosurgical diseases, cross-validate information acquired through different techniques, and gain a better understanding of the relationship between these different brain signals. (dana-farber.org)
Viral3
- Use of peptide synthesis to probe viral antigens for epitopes to a resolution of a single amino acid. (wikipedia.org)
- Here, we describe a simple procedure that permits the identification of viral T-helper (TH)-cell antigens and epitopes. (nih.gov)
- A number of causal bases of autoimmunity from exposure to viral epitopes is well established, whether the route of administration be by exposure via infection or vaccination. (danielsolis.cz)
Peptides2
- Pepscan is a procedure for mapping and characterizing epitopes involving the synthesis of overlapping peptides and analysis of the peptides in enzyme-linked immunosorbent assays (ELISAs). (wikipedia.org)
- The assay was developed by epitope mapping, using synthetic peptides from the spike, membrane, and nucleocapsid protein sequences of SARS-associated coronavirus. (cdc.gov)
Antigens1
- We present in this book chapter the panning in solution and its advantages over panning with immobilized antigens and give detailed protocols for the panning and screening procedure. (bvsalud.org)
Characterization1
- We offer fully supported contract research, fee-for-services and advanced products for gel-based and gel-free proteome analysis (proteomics), protein identification, protein & peptide sequencing, characterization, analysis and epitope mapping by mass spectrometry (MS). (proteome-factory.com)
Cryptic1
- Investigation of the cryptic nature of the Goodpasture epitopes revealed 2 types of alpha-3.alpha-4.alpha-5(IV) hexamers: the autoantibody reactive M-hexamers and the autoantibody impenetrable D-hexamers. (medscape.com)
Amino2
- [ 4 ] The epitopes, designated EA and EB, are located in the NC1 domain at the amino acid residues 17-31 and 127-141, respectively. (medscape.com)
- Its epitope has been mapped to amino acids 155-183 (5). (ancell.com)
Potent1
- This study demonstrates the potential of V H H-based CAR T for pancreatic cancer therapy and provides an engineering strategy for developing potent CAR T cells targeting membrane-distal epitopes. (nature.com)
Immune3
- Knowing the epitope is a valuable information for the development or improvement of biological products, e.g., diagnostic assays, therapeutic mAbs, and vaccines, as well as for the elucidation of immune responses. (bvsalud.org)
- The new mapping method could be used in the future to analyse the anatomy of different cell types, such as nerve cells and cells of the immune system. (elifesciences.org)
- 2021) A complex immune response to flagellin epitope variation in commensal communities. (oeaw.ac.at)
Generate1
- We also presented a new kinematic dataset in the domain of residential roofing with a data processing pipeline to generate necessary annotations for the supervised training procedure on direct joint angle estimation. (cdc.gov)
Assay1
- The standard assay couples IP to immunoblotting (IP/IB), a procedure severely limited as it is not easily scaled for high-throughput analysis. (biomedcentral.com)
Protein sequence3
- i) Mapping of IgE epitopes and PID: The server searches known IgE epitopes in query protein sequence and will assign as allergen if any segment have high similarity with any known epitope. (researchsquare.com)
- vi)Hyprid approach: The query protein sequence is assign as allergen if any one of the methods ( SVM composition based +mapping of IgE epitopes + ARPs BLAST + MEME/MAST) predicts it as allergen. (researchsquare.com)
- 1. Sharief FS, Lee H, Leuderman MM, Lundwall A, Deaven LL, Lee CL, Li SS (1989) Human prostatic acid phosphatase: cDNA cloning, gene mapping and protein sequence homology with lysosomal acid phosphatase. (nordicmubio.com)
Structural1
- The Golby Lab is a translational multidisciplinary group of investigators focusing on functional brain mapping using both structural and functional imaging techniques to guide neurosurgical planning and intra-operative decision making. (dana-farber.org)
Specificity1
- The specificity of this approach is very high but the disadvantage is it has low sensitivity, as not all IgE epitopes of all allergens are known. (researchsquare.com)
Genes1
- Mapping of the genes encoding human protein matches to pathways point to targets that could explain the observed presentation of symptoms in COVID-19 disease. (danielsolis.cz)
Recognize1
- γ interferon expression in CD8+ T cells is a marker for circulating cytotoxic T lymphocytes that recognize an HLA A2-restricted epitope of human cytomegalovirus phosphoprotein pp65. (bdbiosciences.com)
Interactome2
- Researchers from the University of Toronto have created a map of the protein interactions of 19 members of the ATP-binding cassette transporter class in a species of yeast, combining this data with previously-reported ABC transporter interactions into a comprehensive "interactome. (genomeweb.com)
- The group went on in its recent study to investigate two potentially interesting associations revealed in the yeast ABC transporter interactome map: physical interactions of ABC transporters with one another and the interaction of these molecules with members of the zinc transport system. (genomeweb.com)
Sensitivity1
- We show here that FLIP displays similar sensitivity to the standard IP/IB procedure but is amenable to high-throughput analysis. (biomedcentral.com)
Proteomics1
- 2021) Quality standards in proteomics research facilities: Common standards and quality procedures are essential for proteomics facilities and their users. (oeaw.ac.at)
Target3
- Most of the techniques for epitope mapping rely on the presentation of the target, or parts of it, in a way that it can interact with a certain mAb. (bvsalud.org)
- Thus, the design of CARs needs to consider both the epitope within the target and the nature and length of the spacer and TM regions. (nature.com)
- Important for the map's potential usefulness in future clinically-oriented research, 50 percent of the interactions mapped involve a target with an identifiable human ortholog, about 40 percent of which are known to be associated with human disease. (genomeweb.com)
Human2
- We sought to identify an altered peptide ligand (APL) based on the endogenously expressed synovial auto-epitope of human cartilage glycoprotein-39 (HC gp-39) for modulation of cognate, HLA-DR4-restricted T cells. (biomedcentral.com)
- Stagljar said that his team is interested in following up on some insights from the map that are promising for human pharmaceutical research. (genomeweb.com)
Bound1
- The hybridomas all respond to the HC gp-39(263-275) epitope when bound to HLA-DR4(B1*0401) but differ in their fine specificities. (biomedcentral.com)
Molecules2
- The Goodpasture epitopes are structurally sequestered by the adjacent alpha-4(IV)NC1 and alpha-5(IV)NC1 molecules. (medscape.com)
- The group hopes the map, published online this week in Nature Chemical Biology , will be a resource for future applied research, providing insight into these molecules' roles in a variety of cellular processes with implications for drug development and other clinical areas. (genomeweb.com)
Therapeutic2
- Not for use in diagnostic or therapeutic procedures. (bdbiosciences.com)
- This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. (nordicmubio.com)
Cell3
- T-cell epitope mapping by flow cytometry. (bdbiosciences.com)
- Dynamic Organellar Maps enable the proteome-wide analysis of physiological protein movements, without requiring any reagents specific to the investigated process, and will thus be widely applicable in cell biology. (elifesciences.org)
- Epitope mapping identified T cell and B cell epitopes with low binding energies, ensuring their immunogenicity and safety. (htlv.net)
Membrane1
- In the procedure, the nucleic acid mixture is blotted to a membrane where the hybridization is carried out. (web.app)
Method3
- We have developed and applied Dynamic Organellar Maps, a proteomic method that allows global mapping of protein translocation events. (elifesciences.org)
- They harnessed that method in this recent study to build the yeast ABC transporter map. (genomeweb.com)
- Our method leveraged the volumetric pose representation and mapped the rotation representation to a continuous space where each rotation was uniquely represented. (cdc.gov)
Involves1
- Dr. Golby's research involves the application of brain mapping techniques to neurosurgical patients as well as neuroscientific investigations into the brain basis of neurologic functions. (dana-farber.org)
Specific1
- Another platform utilizes an identified cancer-specific immunodominant glycopeptide epitope in MUC1 [64], a heavily glycosylated mucin known to be Inhibitors,research,lifescience,medical associated to several cancer types including breast and ovarian cancer. (srcinhibitors.com)
Production1
- All our procedures and production records are fully documented, allowing complete traceability. (inotivco.com)
Functional2
- The Golby Lab pursues translational research multi-modality image-guided neurosurgery with a particular interest in functional brain mapping. (dana-farber.org)
- This work aims to integrate information acquired from pre-operative brain mapping with intra-operative brain mapping and intra-operative imaging to define functional brain anatomy for surgical planning. (dana-farber.org)
Research2
- The SOPs (standard operating procedures) are applied to all standard analyses in fee-for-services as well as research projects. (proteome-factory.com)
- Evidence extracted from research with fungus and indicate the fact that initiation of DNA replication is a multistep procedure. (pkc-inhibitor.com)
Multiple1
- by the use of chemical labels and multiple-step procedures. (srcinhibitors.com)
Protocols1
- The group described the procedure in Nature Protocols in 2010. (genomeweb.com)
Develop2
- Our goal is to develop a procedure which provides the best balance between yield, purity and cost. (inotivco.com)
- As our understanding and testing procedures develop, more people are being classified as suffering from some type of dementia. (medscape.com)
Conditions1
- Produced in serum-free medium and purified by proprietary chromatography procedure under mild conditions. (abcam.com)