• Like JC-10™, TMRE and TMRM uptake in mitochondria is driven by the mitochondrial membrane potential. (stratech.co.uk)
  • 11 Nicholls D.G. Calcium transport and proton electrochemical potential gradient in mitochondria from guinea-pig cerebral cortex and rat heart. (thieme-connect.de)
  • 15 Castilho R.F., Meinicke A.R., Alemida A.M., Hermes-Lima M., Vercesi A.E. Oxidative damage of mitochondria induced by Fe(II)citrate is potentiated by Ca 2+ and includes lipid peroxidation and alterations in membrane proteins. (thieme-connect.de)
  • it exhibits potential-dependent accumulation in active mitochondria giving red fluorescence with 488 nm excitation. (bitesizebio.com)
  • Fluorescent probes were used to selectively stain mitochondria. (nih.gov)
  • This protective effect may be mediated by inhibiting the mitochondrial membrane permeability transition and reducing the production of reactive oxidative species in mitochondria. (nih.gov)
  • Red fluorescence indicates normal membrane potential or health mitochondria. (dojindo.com)
  • In addition, gain and loss-of-function studies with miR-206 mimics and inhibitors showed that inhibition of miR-206 in hypoxic MSCs promoted the migration ability of the cells, prevented cell apoptosis, and protected membrane potential of mitochondria, while the benefits were all blocked by Pim-1 inhibitor. (biomedcentral.com)
  • We offer a wide assortment of fluorescent probes for analyzing aspects of normal mitochondrial activity in live cells, including reactive oxygen species (ROS) production, mitochondrial membrane potential and calcium flux. (stratech.co.uk)
  • Dr. Tsien first developed fluorescent probes for calcium monitoring in viable cells. (dojindo.com)
  • Grinvald A, Hildesheim R, Farber IC, Anglister L (1982) Improved fluorescent probes for the measurement of rapid changes in membrane potential. (yale.edu)
  • When administrated orally, Arnica 30cH protects against hepatic mitochondrial membrane permeabilization induced by Ca 2+ and/or Fe 2+ -citrate-mediated lipid peroxidation and fragmentation of proteins due to the attack by reactive oxygen species. (thieme-connect.de)
  • Fendiline demonstrated depolarization of mitochondrial membrane potential, with increased reactive oxygen species production in amlodipine and fendiline treated Leishmania , but without plasma membrane disruption. (hindawi.com)
  • Flow cytometry was used to measure the membrane potential and the production of reactive oxidative species. (nih.gov)
  • Elevated levels of solute carrier family 14 member 1 expression induced mitochondrial reactive oxygen species accumulation, diminished the intracellular adenosine triphosphate level, and destroyed both mitochondrial membrane potential integrity and mitochondrial morphology. (biomedcentral.com)
  • Pretreatment with R1-MVs inhibited H 2 O 2 -mediated apoptosis in HaCaT cells by suppressing the loss of mitochondrial membrane potential and reactive oxygen species (ROS) production. (biomedcentral.com)
  • Potentiometric dyes for measuring mitochondrial membrane potential. (stratech.co.uk)
  • According to its abstract, the patent protects an improved method for measuring membrane potential using compounds of a given formula as potentiometric probes. (genomeweb.com)
  • Grinvald A, Salzberg BM, Lev-Ram V, Hildesheim R (1987) Optical recording of synaptic potentials from processes of single neurons using intracellular potentiometric dyes. (yale.edu)
  • We examined the bioelectric properties (resting potential gradients in the epidermis) of Xenopus laevis froglets undergoing hindlimb amputation and observed that the contralateral (undamaged) limb exhibits apparent depolarization signals immediately after the opposite hindlimb is amputated. (biologists.com)
  • The Swarm delivers variable intensity blue-light optogenetic stimulation to enable membrane depolarization and red or lime-light excitation to enable fluorescence detection of the resulting changes in membrane potential or calcium levels, respectively. (frontiersin.org)
  • The results indicate that emission from dental light curing units can affect the oral mucous membrane and may reduce its functional abilities. (who.int)
  • Кандидоз (шкірно-слизовий) Candidiasis is skin and mucous membrane infection with Candida species, most commonly Candida albicans . (msdmanuals.com)
  • Apoptosis consists of an orderly sequence of events characterized by cell shrinkage, increased cell permeability, changes in membrane asymmetry, chromatin condensation, DNA fragmentation, and cell blebbing. (bitesizebio.com)
  • A distinctive feature of the early stages of apoptosis is the collapse of the mitochondrial membrane potential. (bitesizebio.com)
  • Shift of fluorescence emission from red to green of the JC-1 Fluorescent Probe indicating loss of mitochondrial membrane potential associated with apoptosis in treated cells compared to untreated cells. (bitesizebio.com)
  • Moving from early to intermediate stages of apoptosis, you can measure the activation of caspase enzymes, changes in cell membrane permeability, and changes in cell membrane asymmetry. (bitesizebio.com)
  • The far-red fluorescent viability probe DRAQ7™ can be utilized in live-cell assays to help establish levels of cellular toxicity, apoptosis induction and cell health usually combined with an "all cell" dye and/or with a mitochondrial membrane potential probe. (biostatus.com)
  • Also, overexpression of Pim-1 inhibited cardiomyocyte apoptosis and protected mice from infarction injury, which revealed the potential cardioprotective role of Pim-1. (biomedcentral.com)
  • Dojindo offers a wide selection of fluorescent intracellular ion probes for calcium, proton, zinc, and chloride ions. (dojindo.com)
  • Their fluorescent signal intensities or wavelengths change because each intracellular calcium probe has a calcium-selective chelator and a fluorescent moiety in its molecule. (dojindo.com)
  • In designing calcium probes, the BAPTA structure is required as the calcium-binding moiety. (dojindo.com)
  • To prepare a calcium probe, which shifts its fluorescent wavelength and changes its fluorescent intensity upon calcium chelation, a suitable fluorescent part must be selected. (dojindo.com)
  • Calcium channel blockers (CCBs) are a class of drugs that act by selective inhibition of calcium influx through cellular membranes. (hindawi.com)
  • As a demonstration of the versatility of the Swarm, we also developed an assay measuring cardiac action potential and calcium waveform properties simultaneously under paced conditions using human induced pluripotent stem (iPS) cell-derived cardiomyocytes as an additional counter screen for cardiac toxicity. (frontiersin.org)
  • Molecular Devices has been awarded US Patent No. 6,852,504 , "Method for measuring cellular transmembrane potential changes. (genomeweb.com)
  • The collection membrane filters the biological sample, trapping cellular elements of interest. (genomeweb.com)
  • The change in cellular energetic state was studied using rhodamine B as a probe to estimate mitochondrial membrane potential (ΔΨm). (thescipub.com)
  • The effects of PAT on protein thiol levels were examined by reacting cellular proteins with the fluorescent thiol labeling probe monobromobimane. (cdc.gov)
  • Native membrane derived polymer-supported lipid bilayers (nSLBs) are poised to bridge the gap between live cell experiments and traditional model membrane architectures that by offering a combination of accessibility by surface sensitive analytical instrumentation and a composition which more closely resembles cellular membranes by displaying a diversity of endogenous membrane proteins, lipids, and carbohydrates. (lu.se)
  • One is an indirect approach that involves artificially controlling the membrane potential of cells, but it comes with problems of changing the acidity of the surrounding environment or causing unwanted misfiring of neurons. (neurosciencenews.com)
  • Antic S, Major G, Zecevic D (1999) Fast optical recordings of membrane potential changes from dendrites of pyramidal neurons. (yale.edu)
  • Colbert CM, Pan E (2002) Ion channel properties underlying axonal action potential initiation in pyramidal neurons. (yale.edu)
  • Connors BW, Prince DA (1982) Effects of local anesthetic QX-314 on the membrane properties of hippocampal pyramidal neurons. (yale.edu)
  • The Drosophila follicle-cell epithelium provides an appropriate model system for studying the potential role of electrochemical signals, like intracellular pH (pH i ) and membrane potential (V mem ), during development. (biomedcentral.com)
  • 3C or Hi-C based approaches require crosslinking proteins with genomic DNA for probing proximity between genomic loci. (springernature.com)
  • While many studies have been focused on the membrane structure, the dynamics of such systems are crucial for the function of the membrane including membrane bound proteins. (lu.se)
  • Due to the lack of detergent solubilization and reconstitution steps, the nSLBs created using this approach contain essentially all of the native lipids, as well as the membrane-associated proteins and carbohydrates from the donor membrane. (lu.se)
  • The special issue illustrates a diverse range of technologies using nanomaterials and nanoparticles such as graphene, magnetic particles and nanowires, as well as molecular probes and quantum dots. (biomedcentral.com)
  • Almey A, Cannel E, Bertram K, Filardo E, Milner TA, Brake WG (2014) Medial prefrontal cortical estradiol rapidly alters memory system bias in female rats: Ultrastructural analysis reveals membrane-associated estrogen receptors as potential mediators. (concordia.ca)
  • The Plasma Membrane Electric Potential in Yeast: Probes, Results, Problems, and Solutions: A New Application of an Old Dye? (intechopen.com)
  • For a long time, estimations and actual measurements of the electric plasma membrane potential (PMP) in whole yeast cells have been the subject of studies by several groups without reliable results. (intechopen.com)
  • Optogenetic assays provide a flexible, scalable, and information rich approach to probe compound effects for ion channel drug targets in both heterologous expression systems and associated disease relevant cell types. (frontiersin.org)
  • Opto-vTrap directly targets transmitters containing vesicles, and it can be used in various types of brain cells, even the ones that do not respond to membrane potential changes. (neurosciencenews.com)
  • This study investigated the protective effects of membrane vesicles derived from D. radiodurans (R1-MVs) against H 2 O 2 -induced oxidative stress in HaCaT cells. (biomedcentral.com)
  • We recently developed a generic method for producing polymer-supported lipid bilayers directly from cell-derived native membrane vesicles (NMVs). (lu.se)
  • EC 2.1.1.6) was determined from rat cDNA and genomic libraries were screened with DNA probes and specific antiserum. (lu.se)
  • The 3D organization of the genome and its relationship to gene expression is explored using a high-throughput platform that probes 1,000 genomic loci along with the transcription of more than 1,000 genes in thousands of single cells. (springernature.com)
  • Camptothecin induced mitochondrial membrane potential changes were measured with JC-10™ (Cat No. 22204 ) and JC-1 (Cat No. 22200 ) in Jurkat cells. (stratech.co.uk)
  • These experiences have placed me at an intersection between preclinical laboratory efforts to understand relationships between immune effector cells in the central nervous system and synaptic repair during neuroinflammation, and the many steps necessary to bring small molecule technologies into existence as potential drugs. (rochester.edu)
  • Olomoucine, roscovitine and purvalanol are examples of CDK inhibitors (CDKIs) designed and investigated for their apoptotic potential on cancer cells ( 3 ). (spandidos-publications.com)
  • Such probes are used to measure membrane potential in live cells, the abstract states. (genomeweb.com)
  • All the cells in the sample volume are collected onto the membrane. (genomeweb.com)
  • Moreover, it is not applicable for use in cells that do not respond to the membrane potential changes, such as glial cells. (neurosciencenews.com)
  • Curcumin also exerts potential anti-inflammatory effects in diverse cell types, including pancreatic cells, chondrocytes, and hepatic cells ( 9 , 10 ). (spandidos-publications.com)
  • To identify pyroptosis in the cells, electron microscopy was used to observe cell morphology, flow cytometry was used to detect the mitochondrial membrane potential, and the LDH release rate was analyzed by microplate reader. (researchsquare.com)
  • Cell morphological feature, mitochondrial membrane potential, LDH release rate, production of IL-1β and expression of Caspase-1/GSDMD all showed that pyroptosis happened more significantly in A549/DDP cells than that in A549 cells after OP-B treatment. (researchsquare.com)
  • Bischofberger J, Jonas P (1997) Action potential propagation into the presynaptic dendrites of rat mitral cells. (yale.edu)
  • Chen WR, Shepherd GM (1997) Membrane and synaptic properties of mitral cells in slices of rat olfactory bulb. (yale.edu)
  • Debarbieux F, Audinat E, Charpak S (2003) Action potential propagation in dendrites of rat mitral cells in vivo. (yale.edu)
  • Far-red fluorescing probe for apoptotic/dead cells in real-time cell health assays. (biostatus.com)
  • In addition, the polyelectrolyte was able to label the membrane of bacteria and living mammalian cells, separately. (umh.es)
  • TMRE (Tetramethylrhodamine ethyl ester, perchlorate) is used to quantify changes in mitochondrial membrane potential in live cells. (biotium.com)
  • Cells bearing MSC characteristics differ in the facets of proliferation and differentiation, which results in their diverse biological potential and applications in tissue regeneration. (mdpi.com)
  • Mitophagy was not detected in untreated cells and the membrane potential was normal. (dojindo.com)
  • However, reduction of membrane potential and mitophagy were observed in treated cells. (dojindo.com)
  • HTMA-PFBT showed lower affinity to zwitterionic membranes as compared to anionic ones, as well as a more external location, near the membrane-aqueous interface. (umh.es)
  • Determination of high mitochondrial membrane potential in spermatozoa loaded with the mitochondrial probe 5,5',6,6'tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocyanine iodide (JC-1) using flow cytometry. (usda.gov)
  • MitoTracker™ Red CMXRos is another useful mitochondrial probe. (bitesizebio.com)
  • Taken together, R1-MVs exert significant protective effects against H 2 O 2 -induced oxidative stress in keratinocytes and have the potential to be applied in radiation-induced oxidative stress models. (biomedcentral.com)
  • JC-10™, a derivative of JC-1, is potential-dependent probe used to determine ΔΨm by flow cytometry, fluorescence microscopy and in microplate-based fluorescent assays. (stratech.co.uk)
  • With its ultra-low toxicity DRAQ7™ can be applied as a viability probe for real-time or long-term, low-dose exposure assays. (biostatus.com)
  • Pyroptosis is a type of programmed cell death that associated with inflammation, which characterized by cell swelling, destruction of membrane structural integrity and cytoplasmic contents release, like interleukin-1β (IL-1β), interleukin-18 (IL-18) and lactate dehydrogenase (LDH) (5). (researchsquare.com)
  • Sterols play a unique role for the structural and dynamical organization of membranes. (mdpi.com)
  • Cell Meter™ JC-10™ assay kits for measuring mitochondrial membrane potential. (stratech.co.uk)
  • PCR assay or culture isolation and proved suitable to probe hydrolysis technology (Applied Biosystems, Foster detect SARS-CoV in clinical specimens. (cdc.gov)
  • A collapse in mitochondrial membrane and redox potential may induce unwanted loss of cell viability and be a cause of various pathologies. (stratech.co.uk)
  • Fluorescence microscopy studies confirmed the interaction of HTMA-PFBT with the model membranes, labelling the lipid bilayer without perturbing its morphology and showing a clear preference towards anionic systems. (umh.es)
  • No obvious hydrophobic signal sequence, membrane-spanning domains, or potential N-glycosylation sites were found in this sequence. (lu.se)
  • Abstract: Provided herein are variants of an archaerhodopsin useful for application such as optical measurement of membrane potential. (harvard.edu)
  • Probing the potential of apigenin liposomes in enhancing bacterial membrane perturbation and integrity loss. (bvsalud.org)
  • In addition, curcumin contributed to cell survival, as indicated by the restoration of the mitochondrial membrane potential (MMP) and the inhibition of the mitochondrial biogenesis induced by HFFA. (spandidos-publications.com)
  • Activated Caspase-1/4/5/11 catalyze GSDMD to cleave into GSDMD-N and GSDMD-C fragments, and GSDMD-N can bind and form pores at cell membrane, so as to lead pyroptosis and release IL-1β and IL-18 to extracellular environment (8, 9). (researchsquare.com)
  • Chen WR, Shen GY, Shepherd GM, Hines ML, Midtgaard J (2002) Multiple modes of action potential initiation and propagation in mitral cell primary dendrite. (yale.edu)
  • Christie JM, Westbrook GL (2003) Regulation of backpropagating action potentials in mitral cell lateral dendrites by A-type potassium currents. (yale.edu)
  • Macrophage cell membrane-camouflaged nanocarriers can effectively reduce immune cell clearance and actively target tumors. (biomedcentral.com)
  • In this study, a macrophage cell membrane-camouflaged mesoporous silica nanorod (MSNR). (biomedcentral.com)
  • As cell membranes are both dynamic and compositionally complex, replicating these aspects in a model membrane are essential. (lu.se)
  • Orthopoxviruses and Monkeypox virus were detected in the organs by PCR using consensus primers targeting the virus surface membrane haemagglutinin (HA) genes, while Leptospira species were detected by PCR using primers targeting the rrs and lfb1 genes. (bvsalud.org)
  • Together our results reveal the potential ability of gefitinib to initiate sterile inflammation via two distinct mechanisms, and identified IL-1β and HMGB1 as key determinants of gefitinib-induced inflammation that may provide insights into gefitinib-induced interstitial pneumonitis. (nature.com)
  • However, the potential mechanisms by which it promotes invasion and metastasis have not yet been clarified. (biomedcentral.com)
  • The compact design featuring high power LEDs, large numerical aperture optics, and fast photodiode detection enables all-optical individual well readout of 24-wells simultaneously from multi-well plates while maintaining sufficient temporal resolution to probe millisecond response dynamics. (frontiersin.org)
  • Some of these may have led to novel findings harboring great potential for clinical medicine such as, for example, the early detection/diagnosis of diseases or minimally invasive local treatments/interventions. (biomedcentral.com)
  • More specifically, these lights have a potential application for use in transillumination with white light for the detection of caries and calculus and the examination of existing restorations [12]. (who.int)
  • Conclusion: The initial data from our investigation indicates the presence of Leptospira sp in rodent vectors, Rattus, Lophuromys and Praomys, which are the potential small mammalian reservoirs of this pathogen in Cote d'Ivoire. (bvsalud.org)
  • Exposure to 100 uM PAT for 4 hrs reduced the mitochondrial membrane potential by about 30% as measured by rhodamine 123 retention. (cdc.gov)
  • The calculation works like this: First the potential due to the solute charges (call them rho_solute) in the above described dielectric environment is calculated. (lu.se)
  • Next the potential due to rho_solute is calculated in the vacuum dielectric environment described above. (lu.se)
  • The Swarm can screen ~10,000 wells/day in 384-well format, probing complex pharmacological interactions via a wide array of stimulation protocols. (frontiersin.org)
  • The Swarm was able to record pseudo-action potentials stably across all 24 objectives and provided pharmacological characterization of diverse sodium channel blockers. (frontiersin.org)
  • These probes may be used in combination with other fluorescent indicators such as Indo-1, Fura-2, and Fluo-3, and such probes may be used in microplate reading devices such as FLIPR fluorescent imaging plate readers, flow cytometers, and fluorometers. (genomeweb.com)
  • Recent years have seen strong research efforts on the lipid component of biological membranes. (lu.se)
  • In my talk, I will show you some examples how such biological cues influence the dynamic properties of membranes. (lu.se)
  • It did not affect the reperfusion-induced reduction in mitochondrial membrane potential. (nih.gov)
  • The polyelectrolyte was bound with high affinity to the membrane surface, where it exhibited high fluorescence efficiency and stability. (umh.es)
  • Purvalanol induced mitochondrial membrane potential loss, caspase-7 and caspase-3 activation and PARP cleavage following a 48 h treatment. (spandidos-publications.com)
  • Large untreated wastewater sample volumes may require dividing the sample prior to membrane filtration (due to slow filtration rate) or PEG precipitation (due to centrifuge volume constraints). (cdc.gov)
  • Diagnosis is based on inspection, probing of the enamel surface with a fine metal instrument. (msdmanuals.com)
  • Its fluorescent properties have been studied in aqueous media and in presence of model membranes of different composition, in order to explore its ability to be used as a green fluorescent membrane probe. (umh.es)