• molecular
  • During the last decade, the development of nuclear spin polarization enhanced (hyperpolarized) molecular probes has opened up new opportunities for studying the inner workings of living cells in real time. (mdpi.com)
  • The Canadian team of organic chemists and enzymologists developed drug design motifs, and pursued preparative chemistry, enzymology, molecular biology, and tissue culture studies that were interfaced with biological assays and pharmacokinetic studies carried out in Syntex, Palo Alto, CA. (thefreedictionary.com)
  • These phosphorylation events act as molecular on/off switches that can modulate or regulate the target protein biological function. (allindianpatents.com)
  • Selection and amplification binding assay (SAAB) is a molecular biology technique typically used to find the DNA binding site for proteins. (wikipedia.org)
  • Quenching and dequenching upon interaction with a specific molecular biological target is the basis for activatable optical contrast agents for molecular imaging. (wikipedia.org)
  • In contrast to research using biochemistry, genetics, or molecular biology, where mutagenesis can provide a new version of the organism, cell, or biomolecule of interest, chemical biology probes systems in vitro and in vivo with small molecules that have been designed for a specific purpose or identified on the basis of biochemical or cell-based screening (see chemical genetics). (wikipedia.org)
  • Traditionally genotyping is the use of DNA sequences to define biological populations by use of molecular tools. (wikipedia.org)
  • protein
  • A diverse and large set of data ranging from chemistry leads to biological assays and protein sequencing will now be managed and analysed through the Dotmatics Platform. (thefreedictionary.com)
  • A thermal shift assay quantifies the change in thermal denaturation temperature of a protein under varying conditions. (wikipedia.org)
  • Each method has its strengths and weaknesses but they all struggle with intrinsically disordered proteins without any clearly defined tertiary structure as the essence of a thermal shift assay is measuring the temperature at which a protein goes from well-defined structure to disorder. (wikipedia.org)
  • Isolate the DNA bound protein by EMSA: the DNA bound protein, migrated in acrylamide gel, is isolated by autoradiography as per an Electrophoretic mobility shift assay (EMSA) protocol. (wikipedia.org)
  • The radio labeled probe was incubated with 25 pmol of purified Quox1 homeodomain fusion protein in binding buffer for EMSA. (wikipedia.org)
  • As a discipline, proteomics has moved past rapid protein identification and has developed into a biological assay for quantitative analysis of complex protein samples by comparing protein changes in differently perturbed systems. (wikipedia.org)
  • Chemical biologists are poised to impact proteomics through the development of techniques, probes and assays with synthetic chemistry for the characterization of protein samples of high complexity. (wikipedia.org)
  • To prepare for an assay, the researcher fills each well of the plate with some biological entity that they wish to conduct the experiment upon, such as a protein, cells, or an animal embryo. (wikipedia.org)
  • quantify
  • The transducer or the detector element, which transforms one signal into another one, works in a physicochemical way: optical, piezoelectric, electrochemical, electrochemiluminescence etc., resulting from the interaction of the analyte with the biological element, to easily measure and quantify. (wikipedia.org)
  • Synthesis
  • novel retinol dehydrogenases are useful for modulating the synthesis of retinoic acid and, therefore, are useful for modulating gene expression in numerous important biological systems. (google.com)
  • analyte
  • The template probe is fully complementary to the oligonucleotide analyte and is intended to serve as a substrate for T4 DNA ligase-mediated ligation. (wikipedia.org)
  • The template probe has in addition an additional stretch complementary to a ligation probe so that the ligation probe will ligate onto the 3'-end of the analyte. (wikipedia.org)
  • The ligation of the analyte to the ligation probe makes the method specific for the 3'-end of the analyte, ligation by T4 DNA ligase being much less efficient over a bulge loop, which would happen for a 3' metabolite N-1 version of the analyte, for example. (wikipedia.org)
  • DLA probes are ligated at the 5' and 3' ends of the analyte by the joint action of T4 DNA ligase and T4 polynucleotide kinase. (wikipedia.org)
  • High selectivity for the analyte among a matrix of other chemical or biological components is a key requirement of the bioreceptor. (wikipedia.org)
  • wavelength
  • 3. Device according to claim 1 , further comprising at least one illumination source of a wavelength corresponding to an absorption wavelength of said optical sensing probe. (google.es)
  • 4. Device according to claim 1 , further comprising multiple illumination sources of different wavelengths, said wavelengths corresponding to an absorption wavelength of different optical sensing probes. (google.es)
  • Wavelength-based separation techniques sometimes face significant hurdles because most of the widely used luminescence probes have a very small Stokes shift and a broad luminescence spectrum. (omicsonline.org)
  • involves
  • The discipline involves the application of chemical techniques, analysis, and often small molecules produced through synthetic chemistry, to the study and manipulation of biological systems. (wikipedia.org)
  • concentration
  • A method for the quantitation of the effects on a biological system by its exposure to a substance, as well as the quantitation of the concentration of a substance by some observable effect on a biological system. (thefreedictionary.com)
  • Colorimetric assays are normally used to determine how much concentration of one species there is relative to another. (wikipedia.org)
  • reaction
  • A variety of natural, derivatized and designed hyperpolarized probes has emerged for diverse biological studies including assays of intracellular reaction progression, pathway kinetics, probe uptake and export, pH, redox state, reactive oxygen species, ion concentrations, drug efficacy or oncogenic signaling. (mdpi.com)
  • systems
  • Retinoic acid controls a wide variety of important biological systems by regulating eukaryotic gene expression (Chai et al. (google.com)
  • Digoxigenin may be conjugated to sugars to study glycosylation events, even in biological systems. (wikipedia.org)
  • Some forms of chemical biology attempt to answer biological questions by directly probing living systems at the chemical level. (wikipedia.org)
  • specific
  • During my post-doctorate the research programme developed species and taxon-specific probe chemistry to provide near real-time data for biological oceanography. (waikato.ac.nz)
  • These assays were deployed onto the Environmental Sample Processor (www.mbari.org) to collect and report on primary production, as well as specific species autonomously. (waikato.ac.nz)
  • was to develop a probe to test ballast water and detect the presence of this specific maritime pest. (wikipedia.org)
  • techniques
  • Förster resonance energy transfer, a phenomenon on which some quenching techniques rely Acrylamide and iodide fluorescence quenching as a structural probe of tryptophan microenvironment in bovine lens crystallins. (wikipedia.org)
  • upon
  • Chromagen will develop biological assays and screen CombiChem compounds, while receiving research support and a percentage of future payments received by CombiChem upon outlicensing of drug candidates. (thefreedictionary.com)
  • development
  • Although biological assays have been the cornerstone of endocrinologic measurements, the development of the radioimmunoassay (RIA) completely changed the field of endocrinology (1). (thefreedictionary.com)
  • It includes information on all aspects of drug research and development, from drug discovery to biological assays , pharmacokinetics, clinical trials, and post-market surveillance. (thefreedictionary.com)
  • These approaches include the development of enrichment strategies, chemical affinity tags and probes. (wikipedia.org)
  • technique
  • An alternative technique for separating the luminescence signal from background is to take advantage of the lifetime difference between the fluorescence of the probe and the background photon [ 4 ]. (omicsonline.org)
  • chemical
  • citation needed] Trials have been run to find effective removal processes including physical removal of A. amurensis, which was estimated by workshop participants to be the most effective, safe and politically attractive when compared with chemical or biological control processes. (wikipedia.org)
  • Tools
  • Chemotaxis assays are experimental tools for evaluation of chemotactic ability of prokaryotic or eukaryotic cells. (wikipedia.org)
  • makes
  • By combining the power of random-sequence selection with pooled sequencing, the SAAB imprint assay makes possible simultaneous screening of a large number of binding site mutants. (wikipedia.org)
  • another
  • Genotyping is the process of determining differences in the genetic make-up (genotype) of an individual by examining the individual's DNA sequence using biological assays and comparing it to another individual's sequence or a reference sequence. (wikipedia.org)
  • Another variation to detecting the QR probes is by measuring the fluorescence via a spectrofluorometer. (wikipedia.org)
  • utility
  • While each paper shared the core principles of combining CRISPR mediated perturbation with scRNA-seq, their experimental, technological and analytical approaches differed in several aspects, to explore distinct biological questions, demonstrating the broad utility of this methodology. (wikipedia.org)
  • data
  • Depending on the results of this first assay, the researcher can perform follow up assays within the same screen by "cherrypicking" liquid from the source wells that gave interesting results (known as "hits") into new assay plates, and then re-running the experiment to collect further data on this narrowed set, confirming and refining observations. (wikipedia.org)
  • type
  • Quantitative results are gained by the multiwell type of this probe using 4-8-12-channel pipettes. (wikipedia.org)