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  • proteins
  • Thermal stability of proteins has traditionally been investigated using biochemical assays, circular dichroism, or differential scanning calorimetry. (wikipedia.org)
  • Each method has its strengths and weaknesses but they all struggle with intrinsically disordered proteins without any clearly defined tertiary structure as the essence of a thermal shift assay is measuring the temperature at which a protein goes from well-defined structure to disorder. (wikipedia.org)
  • Selection and amplification binding assay (SAAB) is a molecular biology technique typically used to find the DNA binding site for proteins. (wikipedia.org)
  • quantify
  • The transducer or the detector element, which transforms one signal into another one, works in a physicochemical way: optical, piezoelectric, electrochemical, electrochemiluminescence etc., resulting from the interaction of the analyte with the biological element, to easily measure and quantify. (wikipedia.org)
  • analyte
  • The template probe is fully complementary to the oligonucleotide analyte and is intended to serve as a substrate for T4 DNA ligase-mediated ligation. (wikipedia.org)
  • The template probe has in addition an additional stretch complementary to a ligation probe so that the ligation probe will ligate onto the 3'-end of the analyte. (wikipedia.org)
  • The ligation of the analyte to the ligation probe makes the method specific for the 3'-end of the analyte, ligation by T4 DNA ligase being much less efficient over a bulge loop, which would happen for a 3' metabolite N-1 version of the analyte, for example. (wikipedia.org)
  • DLA probes are ligated at the 5' and 3' ends of the analyte by the joint action of T4 DNA ligase and T4 polynucleotide kinase. (wikipedia.org)
  • High selectivity for the analyte among a matrix of other chemical or biological components is a key requirement of the bioreceptor. (wikipedia.org)
  • techniques
  • Förster resonance energy transfer, a phenomenon on which some quenching techniques rely Acrylamide and iodide fluorescence quenching as a structural probe of tryptophan microenvironment in bovine lens crystallins. (wikipedia.org)
  • The discipline involves the application of chemical techniques, analysis, and often small molecules produced through synthetic chemistry, to the study and manipulation of biological systems. (wikipedia.org)
  • another
  • Genotyping is the process of determining differences in the genetic make-up (genotype) of an individual by examining the individual's DNA sequence using biological assays and comparing it to another individual's sequence or a reference sequence. (wikipedia.org)
  • Another variation to detecting the QR probes is by measuring the fluorescence via a spectrofluorometer. (wikipedia.org)
  • plates
  • instead, separate assay plates are created as needed. (wikipedia.org)
  • Depending on the results of this first assay, the researcher can perform follow up assays within the same screen by "cherrypicking" liquid from the source wells that gave interesting results (known as "hits") into new assay plates, and then re-running the experiment to collect further data on this narrowed set, confirming and refining observations. (wikipedia.org)
  • sensitive
  • The biologically sensitive elements can also be created by biological engineering. (wikipedia.org)
  • The use of QR as a probe is relatively safe, inexpensive, and a sensitive method compared with other fluorescence probes like ethidium bromide or dimeric cyanine dyes. (wikipedia.org)
  • cells
  • Labelled (e.g. fluorochromes) cells are also used, in some assays cells get labelled during transmigration the filter. (wikipedia.org)
  • questions
  • While each paper shared the core principles of combining CRISPR mediated perturbation with scRNA-seq, their experimental, technological and analytical approaches differed in several aspects, to explore distinct biological questions, demonstrating the broad utility of this methodology. (wikipedia.org)
  • makes
  • By combining the power of random-sequence selection with pooled sequencing, the SAAB imprint assay makes possible simultaneous screening of a large number of binding site mutants. (wikipedia.org)
  • tags
  • These approaches include the development of enrichment strategies, chemical affinity tags and probes. (wikipedia.org)
  • type
  • Quantitative results are gained by the multiwell type of this probe using 4-8-12-channel pipettes. (wikipedia.org)
  • particular
  • On the other hand, trifluoroaryldiazirines in particular show favorable stability and photolytic qualities and are most commonly used in biological applications. (wikipedia.org)
  • small
  • An assay plate is simply a copy of a stock plate, created by pipetting a small amount of liquid (often measured in nanoliters) from the wells of a stock plate to the corresponding wells of a completely empty plate. (wikipedia.org)
  • quickly
  • Carbenes produced from diazirines are quickly quenched by reaction with water molecules, and hence yields for photoreactive crosslinking assays are often low. (wikipedia.org)
  • addition
  • In addition to being used as colored indicator, quinaldine red is also used as a fluorescence probe and an agent in bleaching. (wikipedia.org)