Presence stem cells. Medical search. Frequent questions

The defining characteristics of pluripotent stem cells, which include embryonic stem (ES) cells and induced pluripotent stem (iPS) cells, are straightforward: their ability to self-renew and differentiate into ectoderm, mesoderm, and endoderm germ layers. (genengnews.com)
A treatment derived from human embryonic stem cells improves mobility in rats with spinal cord injuries, providing the first physical evidence that the therapeutic use of these cells can help restore motor skills lost from acute spinal cord tissue damage. (sci-info-pages.com)
Hans Keirstead and his colleagues in the Reeve-Irvine Research Center at UC Irvine have found that a human embryonic stem cell-derived treatment they developed was successful in restoring the insulation tissue for neurons in rats treated seven days after the initial injury, which led to a recovery of motor skills. (sci-info-pages.com)
For the study, the UCI team used a novel technique they created to entice human embryonic stem cells to differentiate into early-stage oligodendrocyte cells. (sci-info-pages.com)
Keirstead and his colleagues are currently working on other approaches using human embryonic stem cells to treat chronic injuries and other disorders of the central nervous system. (sci-info-pages.com)
Thoma et al observed that Ngn2 alone is sufficient to induce neuronal differentiation in embryonic stem cells. (bartleby.com)
In this study they used murine embryonic stem cells and transfected with Ngn2 expression and after five days post transfection they observed that cells expressed Tuj1 and MAP2 (both are neuronal markers). (bartleby.com)
Ectopic expression of Ngn2 was sufficient to form mature neurons from embryonic stem cells[5]. (bartleby.com)
Embryonic stem cells have been identified by scientist as a type of stem cell that can advance regenerative medicine. (bartleby.com)
However, the greatest potential embryonic stem cells presents are its ability to change into any of the more than 200 different cell types in the body. (bartleby.com)
Previous work in the embryonic rodent brain suggested that the preferential inheritance of the pre-existing (older) centrosome to the self-renewed progenitor cell is required to maintain stem cell properties, ensuring proper neurogenesis. (elifesciences.org)
Using a novel, recombination-induced tag exchange-based genetic tool to birthdate and track the segregation of centrosomes over multiple cell divisions in human embryonic stem cell-derived regionalised forebrain organoids, we show the preferential inheritance of the older mother centrosome towards self-renewed NPCs. (elifesciences.org)
Scientists are one step closer to that reality, now that they have developed the first printer for embryonic human stem cells. (theglobaldispatch.com)
In a new study, researchers from the University of Edinburgh have created a cell printer that spits out living embryonic stem cells. (theglobaldispatch.com)
Researchers have developed a 3D printer that prints human embryonic stem cells. (theglobaldispatch.com)
Human embryonic stem cells (hESCs) are obtained from human embryos and can develop into any cell type in an adult person, from brain tissue to muscle to bone. (theglobaldispatch.com)
These embryonic stem cells were dispensed through computer-operated valves, while a microscope mounted to the printer provided a close-up view of what was being printed. (theglobaldispatch.com)
BMP4-directed trophoblast differentiation of human embryonic stem cells is mediated through a DeltaNp63+ cytotrophoblast stem cell state. (ca.gov)
Using this knowledge, we have determined that human embryonic stem cells, when treated with the chemical BMP4, undergo this 'cytotrophoblast stem cell state' before becoming fully functional placental cells. (ca.gov)
Stem cells, and in particular human embryonic stem (ES) cells, have earned a reputation for being labour intensive and difficult to grow and control in culture. (ddw-online.com)
Progenitor cells are similar to embryonic stem cells in their capacity to differentiate into various cell types. (ddw-online.com)
The need to control differentiation of embryonic stem cells in vitro presents another set of challenges. (ddw-online.com)
The study has shown that manipulation of KLF4 expression is also suitable for these cells, as reducing its expression in keratinocytes derived from embryonic stem cells (ESC) improves their proliferation capacity and their ability to reconstruct skin. (cea.fr)
At a purely mechanical level, we demonstrate that the perpendicular alignment of the animal-vegetal (a-v) and embryonic-abembryonic (eb-ab) axes is a result of minimizing the total elastic conformational energy of the entire collection of cells constrained by the zona pellucida. (lu.se)
Based on their origin, there are two main types of stem cells - embryonic stem cells (ES cells) and postnatal or adult stem cells (AS cells). (bvsalud.org)
Embryonic stem cells are stem cells derived from the inner cell mass of an early, preimplantation stage embryo known as a blastocyst. (bvsalud.org)

Upregulated miR-143 in CSCs differentiation promotes prostate cancers cells SCH58261 metastasis by modulating FNDC3B appearance. (exposed-skin-care.net)
The aim of our study was to define the biological role of IGF-I on proliferation, chondrogenic condensation, apoptosis, and differentiation of MSCs into chondrocytes, alone or in combination with TGF-β and in the presence or absence of TGF-β signaling. (elsevierpure.com)
We discovered that activation of BMP signaling promotes the exit of DIPG tumor cells from 'prolonged stem-cell-like' state to differentiation by epigenetically regulating CXXC5, which acts as a tumor suppressor and positive regulator of BMP signaling. (nature.com)
Thus, targeting the prolonged stem-cell-like state of DIPG cells to differentiation has been proposed as a potential strategy for DIPG treatment. (nature.com)
Mouse studies have suggested that Acvr1 G328V cooperates with Hist1h3b K27M and Pik3ca H1047R to generate high-grade diffuse gliomas, specifically by arresting the differentiation of oligodendroglial lineage cells 16 . (nature.com)
What makes characterization challenging is that heterogeneity within a given stem cell population can cause variability in the differentiation into important downstream lineages, such as cardiomyocytes and hepatocytes. (genengnews.com)
Rebar N. Mohammed Hematopoietic stem cells (HSCs) are a rare population of cells that reside mainly in the bone marrow and are capable of generating and fulfilling the entire hematopoietic system upon differentiation. (karger.com)
Likewise, they have the ability to activate the angiogenesis, proliferation, migration, and differentiation of the main cell types involved in skin regeneration. (frontiersin.org)
Despite their crucial role as a link between innate and adaptive immunity, little is known about the modulation of pDC differentiation by other bone marrow (BM) cells. (nih.gov)
In this study, we investigated the modulation of pDC differentiation in Flt-3 ligand (Flt3L)-supplemented BM cultures, using highly purified mesenchymal stem cells (MSCs) that were FACS-isolated from murine BM based on surface marker expression and used after in vitro expansion. (nih.gov)
2016). Natural killer cell-based adoptive immunotherapy eradicates and drives differentiation of chemoresistant bladder cancer stem-like cells. (springer.com)
In a lab dish, hESCs can be placed in a solution that contains the biological cues that tell the cells to develop into specific tissue types, a process called differentiation. (theglobaldispatch.com)
The printer lays down the cells in precisely sized droplets and in a certain pattern that is optimal for differentiation. (theglobaldispatch.com)
We show that, in primary CTB, p63 levels are reduced as cells differentiate into STB, and that forced expression of p63 maintains cyclin B1 and inhibits STB differentiation. (ca.gov)
We also establish that, similar to in vivo events, hESC differentiation into trophoblast is characterized by a p63+/KRT7+ CTB stem cell state, followed by formation of functional KLF4+ STB and HLA-G+ EVT. (ca.gov)
Taken together, our results establish that BMP4-treated hESCs are an excellent model of human trophoblast differentiation, closely mimicking the in vivo progression from p63+ CTB stem cells to terminally differentiated trophoblast subtypes. (ca.gov)
However, the use of bFGF for human ES cell culture, particularly at the high levels used by some for 'feeder-free' culture, must be considered carefully, as bFGF may drive ES cells towards differentiation. (ddw-online.com)
The power of the thiol proteinases to cleave a multitude of substrates in response to calcium mineral activation allows their involvement in a variety of cell processes offering cell motility, sign transduction, apoptosis, cell differentiation and legislation of the cytoskeleton (3). (exposed-skin-care.net)
Optimal differentiation was verified by qPCR and immunofluorescent (IF) staining of cell-type specific markers. (cdc.gov)
Results: qPCR analysis of the differentiation time-course shows a steady increase in markers of terminally differentiated cells, such as club (SCGB1A1), goblet (MUC5AC) and ciliated cells (FoxJ1). (cdc.gov)
The basal cell (stem/progenitor cell) marker (KRT5) shows an initial increase in expression, but did not vary much during differentiation. (cdc.gov)
Abnormal proliferation, clonal expansion, aberrant differentiation, and diminished apoptosis (programmed cell death) lead to replacement of normal blood elements with malignant cells. (msdmanuals.com)

Introduction: Mesenchymal stem cells (MSCs) can be isolated from adult bone marrow (BM), expanded, and differentiated into several cell types, including chondrocytes. (elsevierpure.com)
Mesenchymal stem cells (MSCs) can differentiate into chondrocytes, while mechanical loading has been proposed as alternative strategy to induce chondrogenesis excluding the use of exogenous factors. (nature.com)
Recently, mesenchymal stem cells (MSCs) have been suggested for use in the cell-based treatment of cartilage lesions. (researchgate.net)
Thus, we attempted to present an overview of previously published reports to evaluate the progress and provide molecular basis of mesenchymal stem cells (MSCs) therapy and its application in preclinical and clinical settings, which could aid in designing an effective regenerative therapeutic strategy in the future. (hindawi.com)
However, effective dose and appropriate time of MSCs delivery are the main challenges in the clinical translation of stem cell therapy. (hindawi.com)
The present study had aimed to evaluate the effect of a feeder layer of irradiated MSCs in the in vitro expansion of NK cells. (springer.com)
MSCs were obtained from the bone marrow (BM) cells remaining in the bag and filter used in the transplantation of hematopoietic stem cells. (springer.com)
NK expansion and activation were stimulated by culture with artificial antigen-presenting cells (aAPCs) and IL-2, in the presence or absence of BM-MSCs. (springer.com)
However, cultures without the presence of MSCs showed higher NK proliferation, expression of activation markers (CD16 and NKG2D) and related cytotoxic activity. (springer.com)
In this experimental study, the presence of a feeder layer of irradiated BM-MSCs interfered negatively in the expansion of PB-NKs, limiting their growth and activation. (springer.com)
MSCs have the capacity of multiple differentiations and self-renewal and have been proven to be able to differentiate into a series of cell lineages, such as adipocytes, osteoblasts, chondroblasts, and hepatocyte-like cells (HLCs). (springer.com)
These dental stem cells are considered mesenchymal stem cells (MSCs) and possess different levels of capacities to become specific tissue forming cells. (bvsalud.org)

Hematopoietic stem cell transplantation (HSCT) involves the intravenous infusion of hematopoietic stem cells in order to reestablish blood cell production in patients whose bone marrow or immune system is damaged or defective. (medscape.com)
of graft-versus-host disease, which is observed as a major complication of hematopoietic stem cell transplantation. (karger.com)
The aims of this article are to review the current knowledge regarding therapeutic mechanisms of mesenchymal stem cells in acute liver failure, to discuss recent advancements in preclinical and clinical studies in the treatment of mesenchymal stem cells, and to summarize the methodological improvement of mesenchymal stem cell transplantation in treating liver failure. (springer.com)
Successful treatment of therapy-resistant chronic leukemia using allogeneic stem cell transplantation. (disabled-world.com)
The transplantation of stem cells from a healthy donor (allogeneic) offers the chance of cure for patients with an aggressive form of chronic lymphocytic leukemia (CLL), irrespective of genetic prognostic factors and the prior course of the disease. (disabled-world.com)
The German CLL Study Group proved this in a multi-centric clinical phase II study led by Professor Dr. Peter Dreger, senior consultant and head of the division of stem cell transplantation at the Department of Internal Medicine V at Heidelberg University Hospital. (disabled-world.com)
For a large percentage of patients, highly sensitive tests were conducted regularly to detect any remaining leukemia cells after the transplantation. (disabled-world.com)
Our study is the largest so far for this patient population and proved that allogeneic stem cell transplantation is a promising therapy option for high-risk CLL and has the potential to cure for this otherwise incurable kind of leukemia," says Professor Dreger, head of the study. (disabled-world.com)
Allogeneic stem cell transplantation is a very stressful and risky form of therapy, which could previously not be carried out on the generally older patients affected by CLL. (disabled-world.com)
Allogeneic stem cell transplantation provides durable disease control in poor-risk chronic lymphocytic leukemia: long-term clinical and MRD results of the GCLLSG CLL3X Trial. (disabled-world.com)
Nucleoside analogs such as fludarabine or cladribine pose a risk of stem cell damage and leukemogenesis and should not be used as initial therapy in patients who are candidates for stem cell transplantation (SCT). (medscape.com)
Hepatic sinusoidal obstruction syndrome (SOS) is a severe and life-threatening complication after allogeneic hematopoietic stem cell transplantation (HSCT). (bvsalud.org)

Accordingly, investigations on cellular therapies have therefore moved to progenitor cell populations such as bone marrow derived mesenchymal stem cells (BMSCs), which have the ability to differentiate into cartilage cells 4 . (nature.com)
During human forebrain development, neural progenitor cells (NPCs) in the ventricular zone (VZ) undergo asymmetric cell divisions to produce a self-renewed progenitor cell, maintaining the potential to go through additional rounds of cell divisions, and differentiating daughter cells, populating the developing cortex. (elifesciences.org)
Use of progenitor cell lines can eliminate the need to culture challenging human ES cells. (ddw-online.com)

SCF also increases the survival of various hematopoietic progenitor cells, such as megakaryocyte progenitors, in vitro. (wikipedia.org)
Hematopoietic progenitor cells have also been shown to migrate towards a higher concentration gradient of SCF in vitro, which suggests that SCF is involved in chemotaxis for these cells. (wikipedia.org)
Currently, the only FDA-approved cell-based therapy for cartilage defects involves autologous chondrocyte implantation: chondrocytes harvested from low-contact areas are expanded in vitro and then re-injected directly into the damaged site 1 . (nature.com)
These studies have demonstrated that mechanical forces are able to shape the mesenchymal stem cell (MSC) fate when appropriately applied in in vitro models of cartilage regeneration. (nature.com)
The recent development of stem cell research and the possibility of generating cells that can be stably and permanently modified in their genome open a broad horizon in the world of in vitro modeling. (wjgnet.com)
In addition to the new in vitro systems, in recent years there were also significant advances in the development of new animal models that allows studying the entire cell cycle of human malaria. (wjgnet.com)
Thanks to recent progresses, stem cells have been extensively employed to study Plasmodium liver and blood cycle in vitro . (wjgnet.com)
The aim of this study was to determinate the effect of different chemotherapy protocols used in the clinic on tumor stem cell markers in vitro . (isciii.es)
Every cell type has its own unique needs when grown in vitro and stem cells are no exception. (ddw-online.com)

CSCs derived from both CSCs and non-CSCs give rise to tumors through self-renewal and are able to differentiate into multiple cell types [6]-[9]. (exposed-skin-care.net)
The most widely accepted method for determining whether a population of cells is truly pluripotent is the teratoma assay, that is, verifying in vivo a cell population's ability to differentiate into each of the three germ layers via teratoma formation in mice. (genengnews.com)
However, progenitor cells can only differentiate into a limited number of cell types. (ddw-online.com)
On the same day, the cells in the apical chamber were exposed to air (airlift) to create the ALI and allowed to differentiate for 28 days. (cdc.gov)
A stem cell is commonly defined as a cell that has the ability to continuously divide and produce progeny cells that differentiate (develop) into various other types of cells or tissues. (bvsalud.org)

Results and Conclusions: IGF-I modulated MSC chondrogenesis by stimulating proliferation, regulating cell apoptosis, and inducing expression of chondrocyte markers. (elsevierpure.com)
Single-cell RNA-sequencing (scRNA-seq) analysis of thousands of DIPG tumor cells containing H3K27M mutations showed that most of these tumor cells resemble oligodendrocyte precursor cells (OPC-like)-a cell type that exhibits obviously enhanced proliferation and tumor-propagating potential compared with other cell subsets in tumor samples 9 . (nature.com)
These antibodies are added to live, unfixed cells in a single-step assay that takes only 30 minutes and has no adverse effects on stemness or proliferation. (genengnews.com)
Cell proliferation involves the replication of all cellular contents with the required energy for this to happen. (hindawi.com)
To prevent aberrant cell proliferation, these pathways are tightly regulated. (hindawi.com)
This activation is necessary for both cell proliferation as well as glucose uptake and use. (hindawi.com)
NK cell proliferation, phenotypic expression and cytotoxic activity were evaluated. (springer.com)
A CEA-Jacob team has just published a paper in which it demonstrates the central role of the transcription factor KLF4 in regulating the proliferation of epidermal stem cells and their ability to regenerate this tissue. (cea.fr)

Advertorial: Pluripotent Stem Cell Verification: Is There an Alternative to Teratoma Assays? (genengnews.com)
The GloLIVE™ Human Pluripotent Stem Cell Live Cell Imaging Kit and individual GloLIVE antibodies available from R&D Systems allow you to verify the pluripotency status of your stem cells by monitoring the expression of both positive and negative markers ( Figure 1 ). (genengnews.com)
Malignant transformation usually occurs at the pluripotent stem cell level, although it sometimes involves a committed stem cell with more limited capacity for self-renewal. (msdmanuals.com)

A successful therapy must therefore eliminate these cells known to be highly resistant to apoptosis. (hindawi.com)
The Cancun Riviera facility reflects Dr. Srivastava's belief that stem cell therapy patients will maximize their success rate when they're in a relaxed state of mind. (prnewswire.com)
However, despite having the promising outcome of preclinical studies, the clinical application of stem cell therapy remained elusive due to little or no progress in clinical trials. (hindawi.com)
The objective of this study was to provide a generalized critique for the role of mesenchymal stem cell therapy in ischemic stroke injury, its underlying mechanisms, and constraints on its preclinical and clinical applications. (hindawi.com)
The recent decade has seen encouraging outcomes of mesenchymal stem cell therapy that holds promise to alleviate the burden of neurological disorders Moreover, initial study data of preclinical trials have also indicated the effectiveness, tolerance, and safety of MSC-based therapy [ 10 ]. (hindawi.com)
Herein, we presented an overview of a previously published work regarding the role of stem cell therapy in ischemic stroke and its underlying molecular mechanisms. (hindawi.com)
We aim to provide the basis for establishing a future study to promote the clinical translation of stem cell therapy in ischemic brain diseases. (hindawi.com)
Mora MV, Ibán MAR, Heredia JD, Laakso RB, Cuéllar R, Arranz MG. Stem cell therapy in the management of shoulder rotator cuff disorders. (wjgnet.com)
US-based StemCures is slated to establish a manufacturing lab in Telangana that will focus on stem cell therapy with the vision to create the largest stem cell manufacturing plant in India. (deccanchronicle.com)
Stem cell therapy is a promising new treatment for a variety of conditions, and I am confident that StemCures will provide high-quality care to patients in India. (deccanchronicle.com)
I look forward to working with the clinic to make stem cell therapy more accessible to patients in India. (deccanchronicle.com)
StemCures is a medical clinic in Ohio that specializes in stem cell therapy. (deccanchronicle.com)
The clinic also has a team of experienced and knowledgeable physicians who can help patients make informed decisions about stem cell therapy. (deccanchronicle.com)
Stem cell therapy is a non-surgical, minimally invasive procedure that uses the patient's own stem cells to treat a variety of conditions. (deccanchronicle.com)
Understanding of molecular mechanisms in natural killer cell therapy. (springer.com)
2014). Human NK cells: From surface receptors to the therapy of leukemias and solid tumors. (springer.com)
We used the pancreatic adenocarcinoma cell line PANC-1 as a model to apply different chemotherapeutic protocols (monotherapy and combined therapy) using 5-Fluorouracil, Oxaliplatin, Irinotecan, Gemcitabine and Abraxane. (isciii.es)
This work has been extended to other types of cells of interest for cutaneous cell therapy. (cea.fr)
2. University of Évry, University of Paris-Saclay, INSERM U861, Institute for Stem Cell Therapy and Exploration of Monogenic Diseases (I-Stem), 91100 Corbeil Essonne, France. (cea.fr)
3. Centre for the Study of Stem Cells (CECS), Institute for Stem Cell Therapy and Exploration of Monogenic Diseases (I Stem), 91100 Corbeil Essonne, France. (cea.fr)
In this context, an objective measure is needed, therefore the index of vaccination against coronavirus disease 2019 (COVID-19) in B-cell lymphomas treated with antibody therapy against CD20 (including after the completion of therapy) was examined. (bvsalud.org)
A total of 40 patients with B-cell lymphoma during or after antibody therapy against CD20 were vaccinated twice with the BNT162b2 messenger RNA (mRNA) COVID-19 vaccine (Pfizer, Inc. and BioNTech SE. (bvsalud.org)
SCID is fatal in the first 2 years of life unless the patient is treated with extremely restrictive isolation, hematopoietic stem cell transplant or therapy that replaces the abnormal gene or gene product. (lu.se)

They also showed a marked reduction in the number of neural stem progenitors in DG. (bartleby.com)

SCF may serve as guidance cues that direct hematopoietic stem cells (HSCs) to their stem cell niche (the microenvironment in which a stem cell resides), and it plays an important role in HSC maintenance. (wikipedia.org)
Through them, the cells modify their microenvironment and the behavior of neighboring cells. (frontiersin.org)
In contrast, cancer cells shift their metabolism toward lactate production even in the presence of oxygen [ 4 ], partly through genetic modifications that stabilize the transcription factor Hypoxia Inducible Factor (HIF) involved in the adaptation of the cells to hypoxia, under nonhypoxic conditions as well as generating an adaptive response to the hypoxic microenvironment (Figure 1 ). (hindawi.com)
An ACS-funded researcher learns how cells that typically kill infected and diseased cells switch sides in the tumor microenvironment to help TNBC grow. (cancer.org)
The tumor microenvironment consists of a variety of cells, a meshwork of fibers, and blood vessels that surround a tumor. (cancer.org)
Compared to the microenvironment of other subtypes of breast cancer, the microenvironment around a triple-negative tumor has a large number of immune cells. (cancer.org)
The presence of immune cells in the microenvironment suggests that immunotherapy may be effective against TNBC. (cancer.org)

We used the CliniMACS Plus system located in our Cell and Tissue. (karger.com)
The easy accessibility of hematopoietic cells, which are not tightly embedded in tissue, has supported. (karger.com)
43 Therefore, cell sheets with enhanced thickness and mechanical properties obtained through appropriate cyclic mechanical stimulation possess great potential for bone tissue engineering applications. (researchgate.net)
Cell sheet-based scaffold-free technology holds promise for tissue engineering applications and has been extensively explored during the past decades. (researchgate.net)
Findings from this study reveal that thermo-responsive elastomer, together with mechanical conditioning, can potentially be applied to prepare high-quality cell sheets for bone tissue engineering. (researchgate.net)
Typically, rapidly proliferating tumor cells have glycolytic rates up to 200 times higher than those of their normal tissue of origin, even in the presence of oxygen [ 3 ]. (hindawi.com)
In the rats treated seven days after the injury, myelin tissue formed as the oligodendrocyte cells wrapped around damaged neurons in the spinal cord. (sci-info-pages.com)
Although the oligodendrocyte cells survived in the chronic injury sites, they could not form myelin because the space surrounding neuron cells had been filled with scar tissue. (sci-info-pages.com)
In the adult brain, the Tlx protein is responsible for generating new neurons from tissue stem cells. (dkfz.de)
As a result, cell division activity in the subventricular zone increased, the cells left their habitual environment called stem cell niche, and started forming glioblastoma-like tissue lesions. (dkfz.de)
Previously, we showed that exosomes derived from human adipose tissue-derived mesenchymal stem cells (ASC-exosomes) attenuate AD-like symptoms by reducing multiple inflammatory cytokine levels. (mdpi.com)
Mariano García Arranz has the following conflict of interest: MGA is inventor on 2 patents entitled "Identification and isolation of multipotent cells from non-osteochondral mesenchymal tissue" (10157355957US) and "Use of adipose tissue-derived stromal stem cells in treating fistula" (US11/167061). (wjgnet.com)
When injected into an injured or diseased area, stem cells can help to repair tissue, reduce inflammation, and promote healing. (deccanchronicle.com)
Imagine if you could take living cells, load them into a printer, and squirt out a 3D tissue that could develop into a kidney or a heart. (theglobaldispatch.com)
Human skin completely renews itself every month thanks to the presence of stem cells in the deepest layer, which generate all the upper layers of this tissue. (cea.fr)
The discoveries of a French research team from the CEA, INSERM and the University of Paris, produced in collaboration with I-Stem, the AFM-Téléthon laboratory, and the University of Évry, opens perspectives for regenerative cutaneous medicine, in particular for the bio-engineering of skin grafts for tissue reconstruction. (cea.fr)
In the future, keratinocytes produced from pluripotent stem cells could be an alternative to adult stem cells in certain reconstructed tissue bio-engineering applications. (cea.fr)
The key elements of tissue engineering are stem cells, morphogen, and a scaffold of extracellular matrix. (bvsalud.org)
Postnatal stem cells have been sourced from umbilical cord blood, umbilical cord, bone marrow, peripheral blood, body fat, and almost all body tissues, including the pulp tissue of teeth 8 . (bvsalud.org)

Pluripotent stem cells have great potential for use in regenerative medicine and are an important tool for understanding developmental and disease mechanisms. (genengnews.com)
It is a natural evolution," says S Ramaswamy, former CEO of C-Camp and professor at the Institute for Stem Cell Biology and Regenerative Medicine ( InStem ). (indiatimes.com)
The mesenchymal stromal cells (MSC) have immunomodulatory properties and a high regenerative capacity. (frontiersin.org)
Therefore, research on exosomes produced by MSC has been intensified for use in cell-free regenerative medicine. (frontiersin.org)
The potential of regenerative medicine ranges from allowing pancreatic cells to produce insulin for diabetics to reconnecting the nerves in severed spinal cords. (bartleby.com)
This attribute makes them ideal for use in regenerative medicine - repairing, replacing and regenerating damaged cells, tissues or organs. (theglobaldispatch.com)
The remarkable potential of stem cells to generate several hundred differentiated cell types is driving their use for regenerative medicine and for supporting the traditional drug discovery and development process (Figure 1). (ddw-online.com)
This expansion phase involves a risk: it may be accompanied by a quantitative loss or degradation of stem cells, leading to a loss of regenerative potential. (cea.fr)
Literature review: This review summarizes current knowledge, barriers, and challenges in the clinical use of adult stem cells, scaffolds, and growth factors for the development and evaluation of regenerative endodontic therapies. (bvsalud.org)
Regenerative endodontic procedures can be defined as biologically based procedures designed to replace damaged structures, including dentin and root structures, as well as cells of the pulp-dentin complex. (bvsalud.org)
The major areas of research that might have application in the development of regenerative endodontic techniques are (a) postnatal stem cells, (b) scaffold materials, (c) morphogen/growth factors, (d) implantation. (bvsalud.org)
The most valuable cells for regenerative endodontics are postnatal or adult stem cells. (bvsalud.org)

Mast cells are the only terminally differentiated hematopoietic cells that express the c-Kit receptor. (wikipedia.org)

This is where neural or brain stem cells reside, which are responsible for generating new neurons if needed. (dkfz.de)
When the scientists switched off Tlx, there were no more detectable stem cells in the brain and the formation of new neurons ceased. (dkfz.de)
Here we establish a midbrain organoid culture system to study the developmental trajectory from pluripotent stem cells to mature dopamine neurons. (lu.se)
Using single cell RNA sequencing, we identify the presence of three molecularly distinct subtypes of human dopamine neurons with high similarity to those in developing and adult human midbrain. (lu.se)

For Sofie Mohlin, associate professor at the Department of Clinical Sciences, and researcher at Lund Stem Cell Center, the win in the local competition of the Forskar Grand Prix meant a lot. (lu.se)
Johan Jakobsson, a professor at Lund University and research group leader at MultiPark and Lund Stem Cell Center, explains, "LINE-1 retrotransposons are a rich source of genetic sequences that we suspect have shaped the evolution of the human brain, and we now have the tools to explore their role in brain development. (lu.se)

An insight into the biology of ischemic stroke indicates that a stream of molecular events initiates instantly after the onset of ischemic stroke, such as oxidative stress, increased level of intracellular calcium, excitotoxicity, and inflammation which results in apoptotic or necrotic neuronal cell death [ 12 - 14 ]. (hindawi.com)
International Journal of Biochemistry & Cell Biology, 40 , 2335-2340. (springer.com)
Natural killer cell biology: An update and future directions. (springer.com)
This is an important step in placental biology, because it provides a reproducible cell culture model for the study of human placental development and disease. (ca.gov)
We urgently need more research to better understand the basic biology behind the development of endometriosis, including the role of genetics, stem cells, and immunology in the disease. (medlineplus.gov)

Many malignancy therapies including chemotherapies that destroy the bulk of malignancy cells may ultimately fail as they do not get rid of CSCs that then cause a relapse of tumors [10]. (exposed-skin-care.net)
The findings point to the potential of using stem cell-derived therapies for treatment of spinal cord damage in humans during the very early stages of the injury. (sci-info-pages.com)
Recently, a growing number of studies are focusing on mesenchymal stem cell-based therapies for neurodegenerative disorders. (hindawi.com)
This explains why researchers are now focusing attention on developing stem cell therapies using postnatal stem cells donated by the patients themselves or their close relatives. (bvsalud.org)

There are limited numbers of publications about ES cells in pulp regeneration, due to the restricted policies regarding ES cell research over the past few years. (bvsalud.org)

Lately we have discovered miR-200b as an integral regulator of chemoresistance and repair of miR-200b considerably reverses chemoresistance of docetaxel (DTX)-resistant LAD cells by inducing cell routine arrest and apoptosis improvement [19]. (exposed-skin-care.net)
In this paper, we describe the metabolic changes as well as the mechanisms of resistance to apoptosis occurring in cancer cells and cancer stem cells, underlying the connection between these two processes. (hindawi.com)

MiR-21 regulates EMT phenotype and hypoxia-inducible aspect-1α appearance in third-sphere developing breast SCH58261 cancer SCH58261 tumor stem cell-like cells. (exposed-skin-care.net)
After analyzing different tumor stem cell markers (SOX2, OCT4, CD133, CD44 and CD24) in pancreatic cancer cells treated with different chemotherapeutic protocols by means of RT-qPCR, Oxaliplatin and Gemcitabine in monotherapy were the chemotherapies that selected the most cancer stem cells while the FOLFIRI protocol decreased them. (isciii.es)

The stromal cells that surround HSCs are a component of the stem cell niche, and they release a number of ligands, including SCF. (wikipedia.org)
In the bone marrow, HSCs and hematopoietic progenitor cells are adjacent to stromal cells, such as fibroblasts and osteoblasts (Figure 2). (wikipedia.org)
These HSCs remain in the niche by adhering to ECM proteins and to the stromal cells themselves. (wikipedia.org)

Bone marrow-derived stem cells Hematopoietic stem cells Adipogenesis Fibrin gel culture, 3-dimensional cultures Original Paper Cells Tissues Organs 2008;187:186 198 DOI: 10.1159/000111804 Accepted after revision: August 29, 2007. (karger.com)

Cells for HSCT may be obtained from the patient himself or herself (autologous transplant) or from another person, such as a sibling or unrelated donor (allogeneic transplant) or an identical twin (syngeneic transplant). (medscape.com)
The National Marrow Donor Program (NMDP), founded in 1986, and the World Marrow Donor Association (WMDA), founded in 1988, were established to (1) locate and secure appropriate unrelated-donor HSCT sources for patients by promoting volunteer donation of bone marrow and peripheral blood stem cells in the community and (2) promote ethical practices of sharing stem cell sources by need, rather than by geographic location of the donor. (medscape.com)

Mice with SCF or c-Kit mutations have severe defects in the production of mast cells, having less than 1% of the normal levels of mast cells. (wikipedia.org)
However, cancer cells overcome these controls, in particular by acquiring genetic mutations leading to the activation of oncogenes (pten, myc) or loss of tumor suppressors (p53) [ 1 ]. (hindawi.com)
This team in Hong Kong tested a drug named PTC124 to suppress specific genetic mutations in other genetic diseases into the iPSC transformed heart muscle cells. (emailwire.com)
are a group of clonal hematopoietic stem cell disorders unified by the presence of distinct mutations of hematopoietic stem cells. (msdmanuals.com)
Splicing factor mutations are particularly prevalent in MDS, a group of heterogeneous hematological disorders characterized by defective blood stem cells and a high risk of leukemia development. (lu.se)

Scientists from the German Cancer Research Center (Deutsches Krebsforschungszentrum, DKFZ) have now shown for the first time that malignant brain tumors arise directly from brain stem cells. (dkfz.de)
Overproduction of Tlx in mice stimulates the development of malignant brain tumors from brain stem cells. (dkfz.de)
Leukemia is a malignant condition involving the excess production of immature or abnormal leukocytes, which eventually suppresses the production of normal blood cells and results in symptoms related to cytopenias. (msdmanuals.com)
50 × 10 9 /L) not caused by malignant transformation of a hematopoietic stem cell. (msdmanuals.com)
In a study published in the Molecular Cell journal, they explain what makes hematopoietic stem cells acquire malignant traits in cancer. (lu.se)

Shaping of natural killer cell antitumor activity by ex vivo cultivation. (springer.com)
Massive ex vivo expansion of epidermal cells (called keratinocytes) is needed for the production of grafts. (cea.fr)

To account for this high level of functional variation, verifying the pluripotency of stem cells-from the generation of a new cell line through its expansion for an experiment-is crucial. (genengnews.com)
To address these difficulties, R&D Systems has developed new tools that aid in the verification of stem cell pluripotency during derivation, maintenance, and expansion. (genengnews.com)
Moreover, colony morphology is not truly indicative of pluripotency, since cells that are not fully reprogrammed can have similar morphology. (genengnews.com)
Pluripotency of live human stem cell colonies was verified using a NorthernLights™ (NL) 493-conjugated Mouse Anti-Human SSEA-4 Monoclonal Antibody (green) and a NL557-conjugated Mouse Anti-Human TRA-1-60(R) Monoclonal Antibody (red). (genengnews.com)
Despite its broad use as the most common assay for assessing the pluripotency of stem cells, the teratoma assay has limitations. (genengnews.com)
Automatically expanded hiPSCs exhibit normal growth characteristics, and show sustained expression of the pluripotency associated stem cell marker TRA-1-60 over at least 5 weeks (10 passages). (frontiersin.org)
More than 89 percent of the cells were still alive three days later, and also tested positive for a marker of their pluripotency - their potential to develop into different cell types. (theglobaldispatch.com)

Identifying the right cocktail of media conditions, supplements and growth factors that successfully drive stem cells toward a desired lineage on a reproducible basis is a time-consuming, iterative exercise. (ddw-online.com)

This indicates that presence of a single pro-neural factor as well could promote neurogenesis. (bartleby.com)
During human brain development, neural progenitor cells (NPCs) undergo two modes of cell division. (elifesciences.org)
For example, neural progenitor cells derived from a human ES cell line are easily propagated and require less handling than human ES cells. (ddw-online.com)

However, efficient harvest and handling of cell sheets remain challenging, including insufficient extracellular matrix content and poor mechanical strength. (researchgate.net)
Mechanical loading has been widely used to enhance extracellular matrix production in a variety of cell types. (researchgate.net)
We found that the extracellular matrix content and thickness of cell sheet were markedly elevated upon appropriate mechanical conditioning. (researchgate.net)
The cells secrete extracellular vesicles (EV) that may have an endosomal origin, or from evaginations of the plasma membrane. (frontiersin.org)
On the other hand, the "exosome vesicle " is an extracellular particle released from the endosomal compartment of most eukaryotic cells. (frontiersin.org)
The exosome vesicles are a type of extracellular vesicles (EV), which are defined as lipid-bilayer spheroid structures, without replicating capacity, that are released from cells, including both prokaryotes and eukaryotes. (frontiersin.org)
Alternatively, stem cell cultures can be grown on extracellular matrix extracts and supplemented with conditioned medium from mouse fibroblast cultures. (ddw-online.com)

To date, four types of human dental stem cells have been isolated and characterized: (i) dental pulp stem cells (DPSCs) 12 (ii) stem cells from exfoliated deciduous teeth (SHED) 21 (iii) stem cells from apical papilla (SCAP) 31 (iv) periodontal ligament stem cells (PDLSCs) 28 . (bvsalud.org)

Thus, the data described here use a novel genetic approach to birthdate centrosomes in human cells and identify asymmetric inheritance of centrosomes as a mechanism to maintain self-renewal properties and to ensure proper neurogenesis in human NPCs. (elifesciences.org)
We show that silk organoids reproduce key molecular aspects of dopamine neurogenesis and reduce inter-organoid variability in terms of cell type composition and dopamine neuron formation. (lu.se)

The advent of the human induced pluripotent stem cells (hiPSCs) technology offers unprecedented opportunities for disease modeling, personalized medicine, and the development of new therapeutic interventions. (frontiersin.org)
Healthcare researchers and scientists at the Li Ka Shing Faculty of Medicine of the Hong Kong University have successfully demonstrated the utilization of human induced pluripotent stem cells or hiPSCs from the skin cells of the patient for testing therapeutic drugs. (emailwire.com)
The use of mouse feeder layers and animal serum are particularly problematic in the culturing of stem cells for possible therapeutic applications. (ddw-online.com)

Isolation, expansion, and characterization of natural killer cells and their precursors as a tool to study cancer immunosurveillance. (springer.com)

The main risk still remains the "graft-versus-host reaction", in which donor cells attack the patient's own cells, which are foreign to them. (disabled-world.com)

This type of stem cell has the potential to transform drug discovery by offering physiologically relevant cells for tool discovery, compound identification, and target validation. (emailwire.com)

SCF is expressed along the pathways that the germ cells use to reach their terminal destination in the body. (wikipedia.org)
Lipids, amino acids, and nucleotides necessary for the biosynthesis of the daughter cells are mostly provided by intermediate metabolites of these pathways. (hindawi.com)

In fact, fetal HSCs in cell culture are 6 times more sensitive to SCF than adult HSCs based on the concentration that allows maximum survival. (wikipedia.org)
Inclusion of fetal bovine serum in stem cell media further contributes to an illdefined culture system. (ddw-online.com)

The gene encoding stem cell factor (SCF) is found on the Sl locus in mice and on chromosome 12q22-12q24 in humans. (wikipedia.org)
After implantation into the critical-sized calvarial defects of mice, the mechanically conditioned cell sheets significantly promoted new bone formation. (researchgate.net)
Using a molecular-biological trick, the investigators induced an overproduction of Tlx by the brain stem cells of mice. (dkfz.de)

Cells Tissues Organs (2021) 209 (4-6): 209-214. (karger.com)
Cells Tissues Organs (2019) 207 (1): 15-20. (karger.com)
Cells Tissues Organs (2008) 188 (1-2): 139-149. (karger.com)
Cells Tissues Organs (2008) 187 (3): 186-198. (karger.com)
Cells Tissues Organs (2005) 180 (4): 195-203. (karger.com)
The new printing method could be used to make 3D human tissues for testing new drugs, grow organs , or ultimately print cells directly inside the body. (theglobaldispatch.com)
It is crucial to control the transplanted donor immune cells so that the effect is strong enough to eliminate the leukemia cells but not strong enough to lead to complications in other tissues and organs. (disabled-world.com)

A team at King's College London, led by Professor Rachael Pearson, are using mini retinas (retinal organoids), which are grown from stem cells in the lab, to develop patches that could recreate a functioning macula. (medicalxpress.com)
In both groups, the early-stage cells formed into full-grown oligodendrocyte cells and migrated to appropriate neuronal sites within the spinal cord. (sci-info-pages.com)

For instance miR-34a repression causes digestive tract CSCs to execute asymmetric cell department and promotes little girl cells to stay digestive tract CSCs by regulating Notch signaling. (exposed-skin-care.net)

RUNX1-positive leukemia cells in preclinical models. (lu.se)

Soluble and transmembrane SCF is produced by fibroblasts and endothelial cells. (wikipedia.org)
They include endothelial cells, fibroblasts, and keratinocytes. (frontiersin.org)

When co-cultured with human mesenchymal stem cells (hMSCs), the NMs exhibit excellent bioactivity in encouraging hMSCs adhesion. (jove.com)
When co-cultured with human mesenchymal stem cells, the nano-matrix exhibited excellent bioactivity in encouraging cell adhesion. (jove.com)
Therefore, it has the potential to serve as an injectable scaffold to repair bone fractures by promoting cell adhesion and function at the target location. (jove.com)
Our model, which couples gene expression with mechanics, suggests that differential adhesion between different cell types is a critical determinant in robust endoderm formation. (lu.se)
In addition to differential adhesion, two different testable hypotheses emerge when considering endoderm formation: (1) A directional force acts on certain cells and moves them into forming the endoderm layer, which separates the blastocoel and the cells of the inner cell mass (ICM), where the blastocoel simply acts as a static boundary. (lu.se)
2) The blastocoel dynamically applies pressure upon the cells in contact, such that cell segregation in the presence of differential adhesion leads to endoderm formation. (lu.se)

KLF4 is therefore a new molecular target for preserving the functionality of stem cells and making progress in the bio-engineering of skin grafts. (cea.fr)

One of the difficulties encountered in this area is that fact that the keratinocytes obtained do not have all the functions of adult stem cells. (cea.fr)

EMAILWIRE.COM , April 18, 2022 ) The Induced Pluripotent Stem Cells Market is slated to grow on a stellar note going forward. (emailwire.com)

Each of those cell sources has specific advantages and disadvantages, and each has found particular clinical applications. (medscape.com)
While this technology is being considered as a breakthrough in clinical stem cell research, the team at Hong Kong University is collaborating with drug companies regarding its clinical application. (emailwire.com)
Overall, the presence of mcr-9 was not associated with significant changes in colistin resistance or clinical outcomes, but continued genomic surveillance to monitor for emergence of AMR genes is warranted. (cdc.gov)

The findings will be of interest to developmental neurobiologists, but also more broadly to cell and developmental biologists. (elifesciences.org)

The objective of this article was to evaluate how these chemotherapeutic regimens affect the proportion of cancer stem cells and the expression of stemness markers. (isciii.es)

Anatomy57

Organisms16

Diseases12

Chemicals and Drugs67

Analytical, Diagnostic and Therapeutic Techniques and Equipment53

Phenomena and Processes51

Disciplines and Occupations4

Technology, Industry, Agriculture2

Information Science2

Named Groups1

Health Care1

Embryonic26

Differentiation22

MSCs12

Transplantation12

Progenitor cell3

Vitro9

Differentiate5

Proliferation8

Pluripotent stem3

Therapy23

Progenitors1

Microenvironment7

Tissue19

Regenerative12

Terminally differentiated1

Neurons4

Lund Stem Cell Center2

Biology5

Therapies4

REGENERATION1

Apoptosis2

Tumor stem2

Stromal cells3

Bone marrow derived1

HSCT2

Mutations5

Malignant5

Vivo2

Pluripotency7

Lineage1

Neural3

Extracellular7

Apical1

Neurogenesis2

Therapeutic3

Precursors1

Patient's1

Type of stem cell1

Pathways2

Fetal2

Mice3

Organs7

Grown2

Asymmetric cell1

Preclinical1

Endothelial cells2

Adhesion6

Molecular1

Adult stem1

20221

Clinical3

Findings1

Regimens1