Precursor saccharomyces cerevisiae proteins. Medical search. Frequent questions

The mitochondria of yeast Saccharomyces cerevisiae contains more than 1000 proteins, majority of which are imported from the cytosol. (uni-goettingen.de)
Herein, we used the amenable yeast Saccharomyces cerevisiae to study whether ubiquitin facilitates the expression of the fused eL40 (Ubi1 and Ubi2 precursors) and eS31 (Ubi3 precursor) ribosomal proteins. (123dok.net)
The c-Jun-like transcriptional activator Gcn4p controls biosynthesis of translational precursors in the yeast Saccharomyces cerevisiae. (uni-luebeck.de)
Her interests include understanding the genetic basis of stress tolerance and fermentation attributes of wine yeast Saccharomyces cerevisiae, and applying this knowledge in strain improvement strategies strain improvement strategies (evolutionary engineering, classical breeding, CRISPR/Cas9 gene editing). (edu.au)
The simplest form of RNA silencing, widely known as RNA interference (RNAi), is conserved in diverse eukaryotic species, including the fungal kingdom, but has been lost in the model budding yeast, Saccharomyces cerevisiae [ 15 ]. (biomedcentral.com)
Work by other groups has indicated that an increase in oxidative stress might be a common feature of conditions that arrest cell division in the budding yeast Saccharomyces cerevisiae . (plu.edu)
To explore the scope of RBPs across eukaryotic evolution, we determined the in vivo RBP repertoire of the yeast Saccharomyces cerevisiae and identified 678 RBPs from yeast and additionally 729 RBPs from human hepatocytic HuH-7 cells. (nature.com)
Probiotic Yeast · Saccharomyces cerevisiae var. (vdocuments.net)
Introduction The discovery and study of the budding yeast Saccharomyces cerevisiae var. (vdocuments.net)
The discovery and study of the budding yeast Saccharomyces cerevisiae var. (vdocuments.net)

these proteins include cell cycle proteins, signaling proteins, and protein-processing enzymes. (wikipedia.org)
The C-terminal domain of the 990-amino acid Schizosaccharomyces pombe Spt5 protein, composed of repeats of a nonapeptide motif (consensus sequence TPAWNSGSK), is necessary and sufficient for binding to the capping enzymes in vivo (in a two-hybrid assay) and in vitro. (mskcc.org)
Among all sterol metabolizing enzymes and their corresponding substrates ERG1 ERG7 ERG11 ERG24 ERG25 ERG26 ERG27 were conserved between Saccharomyces cerevisiae and humans such that proteins with high levels of sequence homology performed comparable functions in sterol biosynthesis HMN-214 (Fig. 1 S4A S4B and Table S1). (bioinf.org)
This highly complex 'protein biogenesis' process is assisted by a diverse network of folding catalysts and protein-modifying enzymes and is scrutinized by molecular chaperones and other 'quality control' factors which ensure that only correctly folded and assembled proteins exit the ER and proceed to distal compartments of the secretory pathway. (stanford.edu)
Low titer and insufficient biocatalytic rate of most enzymes require systematic optimization of recombinant catalyst by protein engineering and consequent C-flux improvement of the yeast chassis for sufficient precursor (acetyl-CoA), energy (ATP), and NADH delivery. (thecbggurus.com)
Furthermore, the transcriptional responses of B5 at different temperature by using rice straw as sole carbon sources were also analyzed, based on which the mechanism of lignocellulose degradation at high temperature was revealed that B5 could resist the heat by up-regulating the heat shock proteins (HSP) and then secrete various Carbohydrate-Active enzymes (CAZymes) to realize energy balance. (researchsquare.com)
Only 60% of yeast and 73% of the human RBPs have functions assigned to RNA biology or structural motifs known to convey RNA binding, and many intensively studied proteins surprisingly emerge as RBPs (termed 'enigmRBPs'), including almost all glycolytic enzymes, pointing to emerging connections between gene regulation and metabolism. (nature.com)
We show that this conserved RNA interactome harbours many proteins without previously assigned roles in RNA biology (enigmRBPs), including surprisingly many metabolic enzymes. (nature.com)
These auxiliary proteins include RNases, RNA-modification and - remodeling enzymes, transport factors, and many others whose function is unclear at present. (biomedcentral.com)

In most eukaryotes studied, ubiquitin is encoded by two classes of genes: (i) The first comprises genes coding for a single copy of ubiquitin fused to ribosomal proteins (r-proteins), most commonly eL40 and eS31. (123dok.net)
The CYP71BE genes were screened based on their enrichment patterns at the transcriptomic-proteomic level and biochemically characterized in Saccharomyces cerevisiae WAT11. (bvsalud.org)
The majority of ERG genes downstream of zymosterol (ERG6 ERG2 ERG3 ERG5 and ERG4) showed little or no sequence homology with human genes (KEGG pathways (17)) HMN-214 but instead proteins with unrelated sequence performed comparable enzymatic activities. (bioinf.org)
The network generated for ERG25 ERG26 ERG27 ERG28 proteins (Fig. 3A S4) revealed as expected many interactions reflecting their participation in the linear ergosterol biosynthesis pathway (green circles in Fig. 3A) as well as additional interactions with genes annotated for functions in lipid synthesis and metabolism. (bioinf.org)
Several molecular mechanisms with antifungal agents have been reported for C. albicans where insertions, deletions, and point mutations in genes codifying target proteins are frequently related to the antifungal drug resistance. (intechopen.com)
In the past decade, yeast have been frequently employed to study the molecular mechanisms of human neurodegenerative diseases, generally by means of heterologous expression of genes encoding the relevant hallmark proteins. (microbialcell.com)
In this respect, although obviously lacking a brain with a multicellular neuronal network, simple unicellular yeast are of great value to investigate the effects of overexpression and single point-mutations in genes encoding key proteins on cell physiology and on the function of conserved signaling cascades. (microbialcell.com)
The advent of high throughput -omics has made the accumulation of comprehensive data sets possible, consisting of changes in genes, transcripts, proteins and metabolites. (mdpi.com)

Additionally, CYP71BE206 showed epoxidase activity toward strictosamide, the critical precursor for CPT biosynthesis, both in vitro and in Nicotiana benthamiana. (bvsalud.org)
Biosynthesis of glycosylphosphatidylinositol (GPI) is required for anchoring proteins to the plasma membrane, and is essential for the integrity of the fungal cell wall. (nature.com)
GPI biosynthesis is a conserved process, required for anchoring proteins to the plasma membrane of fungi and thus essential for the integrity of the fungal cell wall in yeasts and molds 4 . (nature.com)
cloned into Saccharomyces cerevisiae, the heterologous biosynthesis was fully embedded as a proof of concept. (thecbggurus.com)

It leads to the discovery of Ribosome-associated Quality Control (RQC) that monitors aberrant translations and decomposes and removes abnormal proteins during synthesis. (u-tokyo.ac.jp)
We conclude that ubiquitin might serve as a cis-acting molecular chaperone that assists in the folding and synthesis of the fused eL40 and eS31 ribosomal proteins. (123dok.net)
Nearly all mitochondrial proteins are encoded by the nuclear genome and imported into mitochondria after synthesis on cytosolic ribosomes. (escholarship.org)
Therefore, there is a strict spatial separation of protein synthesis and degradation of Gcn4p in yeast. (uni-luebeck.de)
TOR is definitely an atypical serinethreonine kinase conserved in all eukaryotes and is usually a crucial regulator of energy-demanding processes for example protein synthesis, the cell cycle, metabolism, and autophagy (2). (idhinhibitor.com)
Our goal is to elucidate the functional networks that coordinate protein synthesis and quality control in the early secretory pathway. (stanford.edu)
Optimization of precursor conversion and cyclization of the bulky ferulic acid precursor by host metabolic engineering and protein engineering may afford the synthesis of even more structurally diverse stilbene compounds. (biomedcentral.com)
Specific modifications commonly found at the junction of the stems of the cloverleaf structure (Figure 1), referred to as the core, elbow or hinge in the three-dimensional structure of most tRNAs, are often associated with functional folding, allowing tRNAs to fold into the common L-shape form critical to ribosomal protein synthesis. (datexis.com)
Given the demand for equimolar amounts of rRNA and ribosomal proteins during ribosome synthesis, it is essential that the transcription of rRNAs and of the mRNAs for ribosomal proteins is coordinated [ 1 ]. (biomedcentral.com)
Over the past few years, high-throughput experiments have provided evidence that the transcription of the auxiliary proteins involved in ribosome synthesis are also co-regulated. (biomedcentral.com)
Lipid synthesis requires bulk carbon skeleton acyl-CoAs, the transport of which depends on the acyl-CoA binding protein (ACBP). (biomedcentral.com)
Before entering lipid synthesis pathways, different kinds of acyl-CoA transporters which acts as the major carrier of acyl-CoAs, such as fatty acid binding protein (FABP), sterol carrier protein 2 (SCP2) and acyl-CoA-binding protein (ACBP), would activate and convert fatty acids to fatty acyl-CoA esters via a reaction catalyzed by fatty acyl-CoA synthetase and transported to various metabolic locations [ 11 ]. (biomedcentral.com)

Multiplexed proteomics is a powerful tool to assay cell states in health and disease, but accurate quantification of relative protein changes is impaired by interference from co-isolated peptides. (biorxiv.org)
Grape must contains a variety of nitrogenous compounds including ammonia (AMM), amino acids (AA), peptides and proteins, but not all of them can be assimilated by yeast. (winemakermag.com)
Naturally, proteins produced in eukaryotic cell have signal peptides that direct them to the export machinery so that the proteins can be secreted from the cell [ 11 ]. (springeropen.com)

The majority of more than 1000 proteins present in mitochondria are imported from nuclear-encoded, cytosolically synthesized precursor proteins. (edu.au)

RNA-binding proteins (RBPs) mediate pivotal cellular functions such as RNA transport, degradation or translation and represent key effectors of post-transcriptional gene regulation. (nature.com)

Complementation of the yeast ybr 159Δ mutant demonstrated that the two KCR proteins are divergent and that only AtKCR1 can restore heterologous elongase activity similar to the native yeast KCR gene. (unl.edu)
The recombinant NcACBP protein was expressed to verify the binding activities of NcACBP in vitro , and the heterologous expression of NcACBP in Δacbp yeast in vivo . (biomedcentral.com)

Each mRNA transcript can serve as template for repeated translation into protein by ribosomes. (berkeley.edu)
The vast majority of proteins entering the secretory pathway are synthesized on ribosomes docked at ER translocons and are co-transationally translocated into the ER lumen. (stanford.edu)
In a separate line of research, proteomic studies of mature plant ribosomes have revealed considerable variability in the protein composition of individual ribosomes. (biomedcentral.com)
Although eukaryotic ribosomes are composed of only four ribosomal RNAs (rRNAs) and around 80 ribosomal proteins, many other proteins are recruited to help deliver ribosomal subunits to the cytoplasm - at the rate of 2,000 or so ribosomes each minute in a growing yeast cell, for example [ 2 , 3 ]. (biomedcentral.com)

She has extensive experience (30+ years) in genetics and micro (molecular) biology of laboratory and industrial Saccharomyces cerevisiae yeasts. (edu.au)
More recently, my research has included Botrytris cinerea (Grey Mould), non- Saccharomyces yeasts and lactic acid bacteria (LAB) both at a fundamental and applied level. (edu.au)

Understanding the role of the ubiquitin fused to ribosomal proteins becomes relevant, as these proteins are practically invariably eS31 and eL40 in the different eukaryotes. (123dok.net)
The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes. (lookformedical.com)
In eukaryotes, a polycistronic 35S pre-rRNA is transcribed in the nucleolus and cleaved into precursors (pre-rRNAs) of mature 18S and 5.8S rRNAs, as well as 25S or 28S rRNAs in yeast and higher eukaryotes, respectively [ 3 ]. (biomedcentral.com)
Although most of the work was done on budding yeast, because of the relative ease of genetic and biochemical studies in this organism, it is safe to assume that most of the observations also apply to higher eukaryotes, as nearly all the proteins involved in ribosome assembly are conserved between yeast and human. (biomedcentral.com)

The TIM23 and the TIM22 complexes in the inner mitochondrial membrane are essential transport systems for proper insertion of inner membrane and matrix proteins in mitochondria. (uni-goettingen.de)
The TOM and the TIM23 complexes cooperate for importing presequence-containing proteins into mitochondria. (uni-goettingen.de)
Together, these suggest the dynamic nature of interactions within the subunits of the PAM complex, as well as between TOM and TIM23 subunits, during the process of translocation of a protein into mitochondria. (uni-goettingen.de)
These precursor proteins are translocated into mitochondria by the TOM complex, a protein-conducting channel in the mitochondrial outer membrane. (escholarship.org)
Our study reveals the detailed molecular organization of the TOM complex and provides new insights about the mechanism of protein translocation into mitochondria. (escholarship.org)
Additionally, plant mitochondria share proteins with plastids, i.e. dual-targeted proteins. (edu.au)
To determine if Atco is also a precursor of COX, we pulse-labeled Cox6 in isolated mitochondria of a cox6 nuclear mutant with COX6 in mitochondrial DNA. (life-science-alliance.org)

and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell. (lookformedical.com)
Small chromosomal proteins (approx 12-20 kD) possessing an open, unfolded structure and attached to the DNA in cell nuclei by ionic linkages. (lookformedical.com)

Ubiquitin is a highly conserved small eukaryotic protein. (123dok.net)
Ubiquitin is a small eukaryotic protein of 76 amino acids whose name (i.e., it occurs ubiquitously) results from its remarkable evolutionary conservation [1]. (123dok.net)
Saccharomyces cerevisiae (frequently known as baker's yeast) has been extensively employed as a eukaryotic model organism for in-depth evaluation of proteome (11), phosphoproteome (12), and acetylome (13). (idhinhibitor.com)

Saccharomyces cerevisiae (/ˌsɛrəˈvɪsi.iː/) (brewer's yeast or baker's yeast) is a species of yeast (single-celled fungus microorganisms). (wikipedia.org)
A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. (lookformedical.com)

A proteomics-guided approach was used to identify and annotate the proteins enriched during the vigorous CPT metabolism period in mature C. acuminata and seedlings. (bvsalud.org)

Recombinant form of urate oxidase (derived from Saccharomyces cerevisiae -synthesized Aspergillus flavus ), which oxidizes uric acid to allantoin (soluble and inactive). (medscape.com)

While the core subunits involved in mitochondrial protein import are well conserved across wide phylogenetic gaps, the accessory subunits of these complexes differ in identity and/or function when plants are compared with Saccharomyces cerevisiae (yeast), the model system for mitochondrial protein import. (edu.au)
Recent proteomic studies in Saccharomyces cerevisiae have identified nearly 200 proteins, other than the structural ribosomal proteins, that participate in the assembly of ribosomal subunits and their transport from the nucleus. (biomedcentral.com)
Classic work in the early 1970s identified a large 90S pre-ribosome, which is eventually converted into the precursors of the 40S and 60S subunits. (biomedcentral.com)
Many of the yeast subunits are homologs to proteins in higher organisms that are found associated with specific nuclear receptors such as THYROID HORMONE RECEPTORS and VITAMIN D RECEPTORS. (bvsalud.org)

This observation lead Henri Boulard to the isolation of a tropical strain of yeast named Saccharomyces boulardii ( Sb ) from lychee and mangosteen fruit , which is nowadays the only commercialized probiotic yeast. (vdocuments.net)

This means that plants provide a valuable model system to study the various regulatory processes associated with protein import and mitochondrial biogenesis. (edu.au)
Most of the seminal studies on the regulatory mechanisms of autophagosome biogenesis were conducted on Saccharomyces cerevisiae or culture cells in vitro ( Hamasaki and Yoshimori, 2010 ). (frontiersin.org)

Furthermore, gene overexpression is also frequently associated to antifungal resistance as well as an increase in the activity of proteins that reduce oxidative damage. (intechopen.com)
2005) In children with acute lymphoblastic leukemia who received high-dose methotrexate, oxidative damage to proteins as well as other factors was related to toxic side effects. (pinestreetfoundation.org)

The concept of recombinant protein from recombinant DNA harnessed the world's most miniature manufacturing factories, living cells. (springeropen.com)
The NcACBP recombinant protein was able to specifically bind acyl-CoA esters in vitro . (biomedcentral.com)

Additionally, both complexes recognise different targeting signals on a precursor protein. (uni-goettingen.de)
The mitochondrial protein import apparatus is composed of a number of multi-subunit protein complexes that recognize, translocate, and assemble mitochondrial proteins into functional complexes. (edu.au)
These differences include distinct protein import receptors in plants, different mechanistic operation of the intermembrane protein import system, the location and activity of peptidases, the function of inner-membrane translocases in linking the outer and inner membrane, and the association/regulation of mitochondrial protein import complexes with components of the respiratory chain. (edu.au)
The two proteins interact to form Atco complexes that serve as the source of Atp9 for ATP synthase assembly. (life-science-alliance.org)
For this we used the yeast sterol pathway proteins as seeds to mine data from large-scale yeast genetic arrays (18) affinity purification and mass spectroscopy resolution of protein complexes (19-21) and protein complementation screens (22) to gain further insight into their function (Physique S4 Table S1 and supplemental Cytoscape file). (bioinf.org)
The 48 kDa subunit, RETINOBLASTOMA-BINDING PROTEIN 4, is also a component of several other protein complexes involved in chromatin remodeling. (lookformedical.com)
It is found as a subunit of protein complexes that are in involved in the enzymatic modification of histones including the Mi2 and Sin3 histone deacetylase complexes and the polycomb repressive complex 2. (lookformedical.com)

Furthermore, a strong positive genetic interaction occurs between Gap1 and phospholipase Cgamma (PLCgamma), an enzyme that generates inositol-1,4,5-trisphosphate, a precursor for IP4 and a second messenger for intracellular Ca2+ release. (sdbonline.org)
We generated a global genetic interaction network for Saccharomyces cerevisiae, constructing more than 23 million double mutants, identifying about 550,000 negative and about 350,000 positive genetic interactions. (thebiogrid.org)
Unexpectedly multiple genetic and protein-protein interactions were HMN-214 also detected between and proteins with Gene Ontology (GO) annotations indicating direct involvement HMN-214 in vesicular transport secretory pathway and cellular localization: of 178 ERG25-interacting proteins 53 had such GO annotations representing a highly significant enrichment (e.g. vesicle-mediated transport p=1.4*10?8) (Fig. 3B). (bioinf.org)
A retinoblastoma-binding protein that is involved in CHROMATIN REMODELING, histone deacetylation, and repression of GENETIC TRANSCRIPTION. (lookformedical.com)
Not only does RNA translate the genetic code decoding it into protein, but it also has various catalytic and regulatory functions yet to be completely elucidated. (datexis.com)

With purified protein and soluble substrates, the exoglucanase activity of the GH7 domain embedded in the Fusion 3 construct in L. starkeyi was significantly higher than that of the GH7 domain in TeTr CBHI expressed alone. (biomedcentral.com)
However, so far, only a few proteins have been identified as being substrates of this complex. (uni-goettingen.de)
Numerous proteins belonging to the carrier family, such as Crc1, Odc1, Yhm2 and Hem25, were confirmed as substrates of the TIM22 complex. (uni-goettingen.de)
Moreover, previously uncharacterised proteins YPR011C and YFR045W were also identified as TIM22 substrates. (uni-goettingen.de)
Together, these results expand our knowledge about the molecular interactions between mitochondrial translocase components during active protein import, as well as increase our repertoire of the TIM22 complex substrates. (uni-goettingen.de)
This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS. (lookformedical.com)
Its substrates include transforming growth factor beta-related proteins, proalbumin, and von Willebrand factor. (eu.org)
Furin (PACE, paired basic amino acid cleaving enzyme, membrane associated receptor protein) is serine endoprotease responsible for processing a variety of substrates (proparathyroid hormone, transforming growth factor beta 1 precursor, proalbumin, pro-beta-secretase, membrane type-1 matrix metalloproteinase, beta subunit of pro-nerve growth factor and von Willebrand factor). (eu.org)

malonyl CoA-acyl carrier protein transacylase, putative [Geobacter sulf. (yeastrc.org)

CYP716 microsomal proteins were prepared and their enzymatic activities were evaluated in vitro. (bvsalud.org)

We analyze the molecular mechanism of quality control mechanisms that suppress abnormal proteins and clarify drug discovery's molecular basis. (u-tokyo.ac.jp)
We have been analyzing the molecular mechanism of quality control mechanisms that suppress the production of abnormal proteins to clarify the molecular basis of drug discovery (Figure 1). (u-tokyo.ac.jp)

Fluorescent protein-tagged AtKCR1 and AtKCR2 were localized to the endoplasmic reticulum, the site of fatty acid elongation. (unl.edu)

In this study we expressed plant DCL4 in Saccharomyces cerevisiae , an RNAi-depleted organism, in which we could highlight the role of dicing as neither Argonautes nor RNA-dependent RNA polymerase is present. (biomedcentral.com)

S. cerevisiae is currently the only yeast cell known to have Berkeley bodies present, which are involved in particular secretory pathways. (wikipedia.org)
This research confirmed that deletion of some regions of the secretory signal sequence significantly improved the efficiency of HIP protein expression in P. pastoris . (springeropen.com)

We have analyzed the phenotypic effects of a genomic ubi1∆ub-HA ubi2∆ mutant, which expresses a ubiquitin-free HA-tagged eL40A protein as the sole source of cellular eL40. (123dok.net)
Ubiquitin functions as a reversible post-translational modifier of proteins to regulate many different cellular processes such as DNA repair, chromatin dynamics, cell cycle regulation, membrane and protein trafficking, endocytosis, autophagy, but most notably proteasome-dependent protein degradation [2-4]. (123dok.net)
Autophagy is the main cellular process responsible for degrading defective organelles and long-lived proteins. (frontiersin.org)
A family of cellular proteins that mediate the correct assembly or disassembly of polypeptides and their associated ligands. (lookformedical.com)

Four CYP71BE proteins exhibited in vitro isoliquiritigenin epoxidase activity. (bvsalud.org)

The prohormone-processing yeast KEX2 protease can act as an intracellular membrane protein or a soluble, secreted endopeptidase. (eu.org)
simultaneously increases plasma concentration and urine excretion of more soluble oxypurine precursors. (medscape.com)

It is generated by proteolytic cleavage of precursor proteins in which it is fused either to itself, constituting a polyubiquitin precursor of head-to-tail monomers, or as a single N-terminal moiety to ribosomal proteins. (123dok.net)
The subtilisin-like proprotein convertases are expressed extensively in mammalian neural and endocrine cells and play a major role in the proteolytic processing of both neuropeptide and peptide hormone precursors. (eu.org)

The convergence of multiple proteome-level changes around the Rsp5 technique indicates a key function of this pathway in theFrom the Novo Nordisk Foundation Center for Protein Investigation, Faculty of Overall health and Health-related Sciences, University of Copenhagen, Blegdamsvej 3, 2200 Copenhagen, Denmark Author's Choice-Final version full access. (idhinhibitor.com)
On the other hand addition of T-MAS or especially its instant precursor FF-MAS towards the tradition medium reduced cancers cell viability (Fig. 2E) and improved cancer cells level of sensitivity to erlotinib (Fig. 2F G). Used amount these data support the interpretation that sensitization to erlotinib can be connected with perturbation of swimming pools of the sterol intermediate metabolite proximally upstream of SC4MOL in the metabolic pathway. (bioinf.org)

The L1 protein of HPV is the major capsid protein and self-assembles into viruslike particles (VLPs). (cdc.gov)
Both are composed of type-specific HPV L1 protein, the major capsid protein of HPV. (cdc.gov)

This impressive feat of transport and sorting is achieved by the combined action of targeting signals on mitochondrial proteins and the mitochondrial protein import apparatus. (edu.au)

The results all together confirm the role of sense/antisense RNA-based regulation of gene expression, expanding the sense/antisense atlas of S. cerevisiae . (biomedcentral.com)

Our initial assays with the L. starkeyi secretome expressing differing Tr EGII domains fused to a chimeric Talaromyces emersonii - T. reesei CBHI ( TeTr CBHI) showed that the complete Tr EGII enzyme, including the glycoside hydrolase (GH) 5 domain is required for increased expression level of the fusion protein when linked to CBHI. (biomedcentral.com)
Furthermore, S. cerevisiae expression of Fusion 3 construct was poor and only minimal activity was observed when acting on the substrate, p NP-cellobiose. (biomedcentral.com)
While expression levels of eL40A-HA from ubi1∆ub-HA are low, eL40A is produced practically at normal levels from the Smt3-S-eL40A-HA precursor. (123dok.net)
Expression of NLS-truncated Gcn4p results in an increased cytoplasmic fraction and an overall stabilization of the protein. (uni-luebeck.de)
To introduce altered Drosophila Gap1 transgenes into flies, the sevenless ( sev ) enhancer/promoter cassette, an eye-specific promoter, was used to drive expression in cone cell precursors at appropriate levels. (sdbonline.org)
d ) Validation of the yeast mRNA interactome using western blotting of input samples and eluate after interactome capture with specific antibodies (ADH1, alcohol dehydrogenase 1, PUB1) or against TAP-tagged proteins (PGK1, phosphoglycerate kinase 1, TDH1, triose phosphate dehydrogenase, TRX2, thioredoxine 2, SHE2, Swi5p-dependent HO Expression 2). (nature.com)
Expression of the L1 protein using recombinant DNA technology produces noninfectious virus-like particles (VLPs). (cdc.gov)

The TIM22 complex is required for the import of polytopic inner membrane proteins which lack a presequence but have internal targeting signals. (uni-goettingen.de)
Membrane proteins must be threaded co-translocationally into the lipid bilayer to become membrane-integrated, often with complex topologies and typically form hetero- or homo- oligomers. (stanford.edu)

Characterization of polysulfides in Saccharomyces cerevisiae cells and finished wine from a cysteine-supplemented model grape medium. (edu.au)

We have identified a cytosolic acyl-CoA binding protein, with highly conserved amino acid residues and a typical acyl-CoA binding domain in N. caninum . (biomedcentral.com)

Its six backbone torsion angles and glycosidic bond between base and ribose provide conformational dynamics not afforded by the peptide bond of proteins and more flexibility than the DNA backbone. (datexis.com)

Here, we use a reporter gene-based screen in Saccharomyces cerevisiae for the discovery of antifungal inhibitors of GPI-anchoring of proteins, and identify the oligocyclopropyl-containing natural product jawsamycin (FR-900848) as a potent hit. (nature.com)

One of the unexpected findings of the Human Genome Project was that over 98% of the human genome does not encode for proteins. (pharmaceuticalintelligence.com)

The Cox6 subunit of Saccharomyces cerevisiae cytochrome oxidase (COX) and the Atp9 subunit of the ATP synthase are encoded in nuclear and mitochondrial DNA, respectively. (life-science-alliance.org)
In Saccharomyces cerevisiae , Atp9 is a mitochondrially encoded subunit that oligomerizes into a 10-subunit ring of the F 1 -F o ATP synthase ( Dautant et al, 2010 ), whereas in humans, Atp9 is encoded by the nucleus and forms an 8-subunit ring ( Watt et al, 2010 ). (life-science-alliance.org)
Although initially discovered as a retinoblastoma binding protein it has an affinity for core HISTONES and is a subunit of chromatin assembly factor-1 and polycomb repressive complex 2. (lookformedical.com)

We will also discuss potential approaches to humanize yeast in order to create modification patterns resembling the situation in mammalian cells, which could enhance the value of Saccharomyces cerevisiae and Kluyveromyces lactis as disease models. (microbialcell.com)

A bioinformatics survey of the Arabidopsis ( Arabidopsis thaliana ) genome has revealed two sequences homologous to YBR159 w encoding a Saccharomyces cerevisiae β-ketoacyl reductase (KCR), which catalyzes the first reduction during VLCFA elongation. (unl.edu)

After mRNA processing, most transcripts are exported to the cytoplasm for translation into protein. (berkeley.edu)
The number of protein products produced by any single mRNA can vary widely. (berkeley.edu)
Proteins shown in red (FDR 0.01) represent the mRNA interactome. (nature.com)
c ) Overlap of mRNA interactome proteins in yeast and HuH-7. (nature.com)

The intestine of Polistes dominula, a social wasp, hosts S. cerevisiae strains as well as S. cerevisiae × S. paradoxus hybrids. (wikipedia.org)
2016) showed that the intestine of Polistes dominula favors the mating of S. cerevisiae strains, both among themselves and with S. paradoxus cells by providing environmental conditions prompting cell sporulation and spores germination. (wikipedia.org)

Ras signaling can be thought of in simple terms as occuring downstream of receptor tyrosine kinases, triggered by association of adaptor proteins with the phosphorylated cytoplasmic tails of these receptors. (sdbonline.org)

We demonstrate that when combined with fast micro- or analytical flow-rate chromatography, Zeno SWATH MS increases protein identification with low sample amounts. (elifesciences.org)
For instance, using 20 min micro-flow-rate chromatography, Zeno SWATH MS identified more than 5000 proteins consistently, and with a coefficient of variation of 6%, from a 62.5 ng load of human cell line tryptic digest. (elifesciences.org)
Using 5 min analytical flow-rate chromatography (800 µl/min), Zeno SWATH MS identified 4907 proteins from a triplicate injection of 2 µg of a human cell lysate, or more than 3000 proteins from a 250 ng tryptic digest. (elifesciences.org)

These proteins have predominantly been defined to contain either four or six transmembrane domains. (uni-goettingen.de)
Dimeric TOM consists of two copies each of five proteins arranged in two-fold symmetry: pore-forming β-barrel protein Tom40 and four auxiliary α-helical transmembrane proteins. (escholarship.org)

Translation quality controls eliminate aberrant proteins to maintain protein homeostasis and normal cell function. (u-tokyo.ac.jp)
Accumulation of aberrant proteins in cells causes various cell dysfunctions. (u-tokyo.ac.jp)
Translation quality controls eliminate and prevent aberrant proteins from maintaining protein homeostasis normal cell function. (u-tokyo.ac.jp)
The ER is the 'port of entry' for proteins destined for the cell surface and beyond. (stanford.edu)
Proteins that control the CELL DIVISION CYCLE. (lookformedical.com)
Proteins found in the nucleus of a cell. (lookformedical.com)
Phenylpropanoids are the precursors to a range of important plant metabolites such as the cell wall constituent lignin and the secondary metabolites belonging to the flavonoid/stilbene class of compounds. (biomedcentral.com)
After cell lysis, polyadenylated RNAs were captured on oligo d(T) beads followed by stringent washes to remove non-crosslinked proteins. (nature.com)
High-risk types (e.g., types 16 and 18) can cause low-grade cervical cell abnormalities, high-grade cervical cell abnormalities that are precursors to cancer, and cancers ( 5 - 7 ). (cdc.gov)

Effect of 'loss of function' mutation in SER in wine yeast: fermentation outcomes in co-inoculation with non-Saccharomyces. (edu.au)

Proteins obtained from the species SACCHAROMYCES CEREVISIAE. (lookformedical.com)
Subsequent research revealed that microRNAs are more dynamic than previously thought, with each precursor being able to generate multiple mature microRNA species known as isomiRs. (pharmaceuticalintelligence.com)

While proteomics typically aims to quantify as many proteins as possible, many of the biological questions that are to be addressed also necessitate the processing of large sample series. (elifesciences.org)
To demonstrate the power of TMTProC, we analyzed a human and yeast interference sample and were able to quantify 13,290 proteins in 24 fractions. (biorxiv.org)

These properties became critical in the production of recombinant proteins that secrete the protein of interest into the external culture environment. (springeropen.com)

Anatomy21

Organisms13

Diseases1

Chemicals and Drugs115

Analytical, Diagnostic and Therapeutic Techniques and Equipment24

Phenomena and Processes89

Disciplines and Occupations1

Technology, Industry, Agriculture3

Information Science4

Health Care3

Yeast Saccharomyces cerevisiae10

Enzymes9

Genes8

Biosynthesis4

Synthesis12

Peptides3

1000 proteins1

Post-Transcriptional1

Heterologous2

Ribosomes4

Yeasts2

Eukaryotes4

Mitochondria7

Chromosomal proteins2

Eukaryotic3

Baker's2

Metabolism1

Aspergillus1

Subunits4

Boulardii1

Biogenesis2

Oxidative damage2

Recombinant Protein2

Complexes7

Genetic5

Substrates8

Putative1

Enzymatic1

Molecular2

Fatty1

Organism1

Secretory2

Cellular4

Vitro1

Soluble2

Proteolytic2

Pathway2

Major capsid protein2

Mitochondrial protein-import1

Gene expression1

Expression7

Membrane2

Characterization1

Cytosolic1

Peptide1

Antifungal1

Encode1

Subunit3

Mammalian cells1

Arabidopsis1

MRNA4

Strains2

Adaptor1

Chromatography3

Transmembrane2

Cell9

Fermentation1

Species2

Quantify2

Secrete1