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ReactionAntibodiesTube precipitinImmunodiffusionSerumAntigen and antibodyPrecipitateTestLaboratoryLungSubstancesSpecificFormationStudiesBloodActivity

Reaction7

• The precipitin reaction provided the first quantitative assay for antibody. (wikipedia.org)
• The precipitin reaction is based upon the interaction of antigen with antibody leading to the production of antigen-antibody complexes. (wikipedia.org)
• To produce a precipitin reaction, varying amounts of soluble antigen are added to a fixed amount of serum containing antibody. (wikipedia.org)
• In this article, Heidelberger detailed his findings of what happened when he conducted a precipitin reaction between the polysaccharides of type III pneumococcus and its homologous antibody (an antibody formed against a specific antigen, here type III pneumococcal polysaccharide). (nih.gov)
• Heidelberger developed this so-called precipitin reaction into a powerful experimental method in immunochemistry. (nih.gov)
• A positive precipitin reaction after two hourse at room temperature almost always insures a titre of anti-human globulin greater than 1 : 5,000 as obtained by the Coombs method. (karger.com)
• The precipitin reaction has been used to locate serum proteins separated by thin-layer gel filtration on Sephadex G-200, thereby greatly increasing the sensitivity of the method. (bmj.com)

Antibodies5

• Aspergillosis precipitin is a laboratory test to detect antibodies in the blood resulting from exposure to the fungus aspergillus . (medlineplus.gov)
• The sample is sent to a laboratory where it is examined for precipitin bands that form when aspergillus antibodies are present. (medlineplus.gov)
• Aerosol exposure to ECMV also induced the formation of specific precipitin antibodies and lymphocyte activation. (aaem.pl)
• Diagnosis is suspected based on history and imaging tests and confirmed by Aspergillus skin testing and measurement of IgE levels, circulating precipitins, and A. fumigatus -specific antibodies. (msdmanuals.com)
• Antibodies that interact with antigens and result in the formation of precipitates are called precipitins. (biologyease.com)

Tube precipitin1

• Antiserum obtained from a rabbit 30 days after the second of two injections, each of c. 100 µg purified virus emulsified with Freund s complete adjuvant, had a titre of 1/1280 in tube precipitin and microprecipitin tests and 1/2560 in ring interface tests ( Brunt & Stace-Smith, 1978 a ). (dpvweb.net)

Immunodiffusion1

• Calf Thymus Extract forms precipitins with autoantibodies that bind Sm, nRNP, La/SS-B, and Jo-1 when tested by immunodiffusion. (calbiotech.com)

Serum4

• Note: 0.25 mL serum is required for one precipitin. (nationaljewish.org)
• The serum precipitin test assists in the diagnosis of HP. (nationaljewish.org)
• Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. (abcam.com)
• Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Human Transferrin and anti-Human Serum. (creativebiomart.net)

Antigen and antibody1

• When the concentration of antigen and antibody molecules is equal, a precipitin band (white) appears at the junction of antigen-antibody complex. (biologyease.com)

Precipitate1

• A precipitin is an antibody which can precipitate out of a solution upon antigen binding. (wikipedia.org)

Test6

• The gel diffusion precipitin test (GDPT) and restriction endonuclease analysis (REA) have commonly been used in the serotyping and genotyping of Pasteurella multocida. (nih.gov)
• Homologous antiserum had a litre of 1/256 in lube precipitin test. (apsnet.org)
• In the real world, in 1901, Uhlenhuth developed the precipitin test to detect human blood. (frontiersin.org)
• A useful and simple screening technique is the capillary tube (1.5-2.5x100 mm.) precipitin test. (karger.com)
• As little as 4% was readily detectable by the Ouchterlony method or precipitin test. (rupress.org)
• He is famous in the annals of forensic science for developing the species precipitin test, known as the Uhlenhuth test, which could distinguish human blood from animal blood in 1901, a discovery which had tremendous importance in criminal justice in the 20th century. (peoplepill.com)

Laboratory1

• In addition, the DACI Laboratory performs hypersensitivity pneumonitis precipitin panels. (hopkinsmedicine.org)

Lung1

• The role of precipitins in allergic lung disease. (cdc.gov)

Substances1

• These defensive substances were named precipitins. (peoplepill.com)

Specific2

• No precipitin line was detected against goat anti-human IgM, Fc 5μ fragment specific or goat anti-human IgA, a chain specific. (jacksonimmuno.com)
• They found that the precipitins were specific to the antagonist injected. (peoplepill.com)

Formation1

• In the zone of equivalence, the formation of precipitin complexes is optimal. (wikipedia.org)

Studies1

• Since some recent studies have demonstrated that chickens are excellent producers of precipitin [3, 4], it seemed reasonable to attempt the production of Coombs reagent by injecting chickens with human gamma globulin. (karger.com)

Blood1

• Blood avian precipitins were strongly positive. (bmj.com)

Activity1

• 6) Precipitin reactions are present in patients with allergic bronchopulmonary aspergillosis, but presence or absence does not reflect disease activity in most cases. (nih.gov)